Prkab1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkab1
Entrez Gene ID	83803
Full Name	protein kinase AMP-activated non-catalytic subunit beta 1
General protein information	Preferred Names protein kinase AMP-activated non-catalytic subunit beta 1 Names 5'-AMP-activated protein kinase subunit beta-1 5'-AMP-activated protein kinase 40 kDa subunit 5'-AMP-activated protein kinase, beta subunit 5-AMP-activated protein kinase beta subunit AMPK beta-1 chain AMPK subunit beta-1 AMPKb protein kinase, AMP-activated, beta 1 non-catalytic subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16
Summary	may facilitate the association of the heterotrimeric AMP-activated protein kinase; may phosphorylate and inactivate enzymes involved in lipid metabolism; may play a role in response to metabolic stress [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006249481.3	XP_006249543.1	5'-AMP-activated protein kinase subunit beta-1 isoform X1
NM_031976.1	NP_114182.1	5'-AMP-activated protein kinase subunit beta-1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PRKAB1	NP_001035001.1
Schizosaccharomyces pombe (fission yeast)	amk2	NP_588485.1
	PRKAB1	XP_001085323.1
Bos taurus (cattle)	PRKAB1	NP_001019729.1
Canis lupus familiaris (dog)	PRKAB1	XP_543421.2
Danio rerio (zebrafish)	prkab1a	NP_001002632.1
Drosophila melanogaster (fruit fly)	alc	NP_610460.1
Mus musculus (house mouse)	Prkab1	NP_114075.1
Rattus norvegicus (Norway rat)	Prkab1	NP_114182.1
Magnaporthe oryzae (rice blast fungus)	MGG_06930	XP_003709669.1
Arabidopsis thaliana (thale cress)	AT4G16360	NP_001031650.2
Xenopus tropicalis (tropical clawed frog)	prkab1	XP_002932123.1
Homo sapiens (human)	PRKAB1	NP_006244.2
Pan troglodytes (chimpanzee)	PRKAB1	NP_001233530.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008195	XP_317273.3
Neurospora crassa	NCU03837	XP_960596.1

Gene Ontology
Bibliography

Related articles in PubMed

Lack of Adipocyte AMPK Exacerbates Insulin Resistance and Hepatic Steatosis through Brown and Beige Adipose Tissue Function.
Mottillo EP, Desjardins EM, Crane JD, Smith BK, Green AE, Ducommun S, Henriksen TI, Rebalka IA, Razi A, Sakamoto K, Scheele C, Kemp BE, Hawke TJ, Ortega J, Granneman JG, Steinberg GR
Cell metabolism24(1)118-29(2016 Jul)

Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.
DiTommaso T, Jones LK, Cottle DL, , Gerdin AK, Vancollie VE, Watt FM, Ramirez-Solis R, Bradley A, Steel KP, Sundberg JP, White JK, Smyth IM
PLoS genetics10(10)e1004705(2014 Oct)

Structural insights into the architecture and allostery of full-length AMP-activated protein kinase.
Zhu L, Chen L, Zhou XM, Zhang YY, Zhang YJ, Zhao J, Ji SR, Wu JW, Wu Y
Structure (London, England : 1993)19(4)515-22(2011 Apr)

Modulation of the activities of AMP-activated protein kinase, protein kinase B, and mammalian target of rapamycin by limiting energy availability with 2-deoxyglucose.
Jiang W, Zhu Z, Thompson HJ
Molecular carcinogenesis47(8)616-28(2008 Aug)

Activation of 5'-AMP-activated kinase with diabetes drug metformin induces casein kinase Iepsilon (CKIepsilon)-dependent degradation of clock protein mPer2.
Um JH, Yang S, Yamazaki S, Kang H, Viollet B, Foretz M, Chung JH
The Journal of biological chemistry282(29)20794-8(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies determine the three-dimensional reconstruction and subunit organization of the full-length rat AMPK (alpha1beta1gamma1) through single-particle electron
Title: Structural insights into the architecture and allostery of full-length AMP-activated protein kinase.

the activities of AMP-activated protein kinase, protein kinase B, and mammalian target of rapamycin by limiting energy availability with 2-deoxyglucose
Title: Modulation of the activities of AMP-activated protein kinase, protein kinase B, and mammalian target of rapamycin by limiting energy availability with 2-deoxyglucose.

metformin and AMPK have a previously unrecognized role in regulating the circadian rhythm
Title: Activation of 5'-AMP-activated kinase with diabetes drug metformin induces casein kinase Iepsilon (CKIepsilon)-dependent degradation of clock protein mPer2.

the inhibition of hepatic gluconeogenesis by AMPK may, in part, be mediated by an immediate early gene response involving EGR1 and its target, DUSP4
Title: Inhibition of gluconeogenesis through transcriptional activation of EGR1 and DUSP4 by AMP-activated kinase.

X-ray diffraction data collected from a selenomethionine-substituted protein.X-ray data collected from a selenomethionine-substituted protein.
Title: Crystallization of the glycogen-binding domain of the AMP-activated protein kinase beta subunit and preliminary X-ray analysis.

The following Prkab1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkab1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa35123	XM_006249481.3 Latest version!	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 1 (Prkab1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa08890	NM_031976.1 Latest version!	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 1 (Prkab1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkab1 ( XM_006249481.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249543.1 Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 1 (Prkab1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35123
Clone ID Related Accession (Same CDS sequence)	XM_006249481.3
Accession Version	XM_006249481.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	5'-AMP-activated protein kinase subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249481.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATA CGAGCAGCGA GCGCGCCGCG CTGGAGCGGC AGGCTGGCCA TAAGACGCCG 
CGGAGGGACA GCTCGGGGGG CACCAAGGAT GGGGACAGGC CCAAGATCCT GATGGACAGC 
CCCGAAGACG CCGACATCTT CCACACCGAG GAAATGAAGG CTCCAGAGAA GGAGGAGTTC 
CTGGCGTGGC AGCACGACCT CGAGGTGAAT GAGAAAGCCC CCGCCCAGGC TCGGCCCACC 
GTATTTCGAT GGACAGGGGG TGGAAAGGAG GTCTACTTGT CTGGATCCTT CAACAACTGG 
AGCAAATTGC CCCTCACTAG AAGCCAAAAC AACTTCGTAG CCATCCTGGA CCTGCCGGAA 
GGAGAGCATC AGTACAAGTT CTTTGTGGAT GGCCAGTGGA CCCACGATCC TTCCGAGCCA 
ATAGTAACCA GCCAGCTTGG CACAGTTAAC AACATCATTC AAGTGAAGAA AACTGACTTT 
GAAGTATTTG ATGCTTTAAT GGTGGATTCC CAAAAGTGCT CCGATGTATC TGAGCTGTCC 
AGTTCCCCCC CAGGACCCTA CCACCAGGAG CCTTACATCT CTAAACCAGA GGAGCGGTTC 
AAGGCCCCGC CCATCCTCCC GCCTCACCTG CTGCAGGTCA TCTTGAACAA GGACACGGGC 
ATCTCTTGTG ATCCAGCGCT GCTTCCGGAG CCCAACCACG TCATGCTGAA CCACCTCTAT 
GCACTCTCTA TCAAGGATGG AGTGATGGTG CTCAGTGCGA CCCATCGGTA CAAGAAAAAG 
TACGTCACCA CCCTCCTCTA CAAGCCCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249543.1
CDS	131..943
Translation	MGNTSSERAALERQAGHKTPRRDSSGGTKDGDRPKILMDSPEDADIFHTEEMKAPEKEEFLAWQHDLEVNEKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSQNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYISKPEERFKAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI

Target ORF information:

RefSeq Version	XM_006249481.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 1 (Prkab1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249481.3

ORF Insert Sequence:

ATGGGCAATA CGAGCAGCGA GCGCGCCGCG CTGGAGCGGC AGGCTGGCCA TAAGACGCCG 
CGGAGGGACA GCTCGGGGGG CACCAAGGAT GGGGACAGGC CCAAGATCCT GATGGACAGC 
CCCGAAGACG CCGACATCTT CCACACCGAG GAAATGAAGG CTCCAGAGAA GGAGGAGTTC 
CTGGCGTGGC AGCACGACCT CGAGGTGAAT GAGAAAGCCC CCGCCCAGGC TCGGCCCACC 
GTATTTCGAT GGACAGGGGG TGGAAAGGAG GTCTACTTGT CTGGATCCTT CAACAACTGG 
AGCAAATTGC CCCTCACTAG AAGCCAAAAC AACTTCGTAG CCATCCTGGA CCTGCCGGAA 
GGAGAGCATC AGTACAAGTT CTTTGTGGAT GGCCAGTGGA CCCACGATCC TTCCGAGCCA 
ATAGTAACCA GCCAGCTTGG CACAGTTAAC AACATCATTC AAGTGAAGAA AACTGACTTT 
GAAGTATTTG ATGCTTTAAT GGTGGATTCC CAAAAGTGCT CCGATGTATC TGAGCTGTCC 
AGTTCCCCCC CAGGACCCTA CCACCAGGAG CCTTACATCT CTAAACCAGA GGAGCGGTTC 
AAGGCCCCGC CCATCCTCCC GCCTCACCTG CTGCAGGTCA TCTTGAACAA GGACACGGGC 
ATCTCTTGTG ATCCAGCGCT GCTTCCGGAG CCCAACCACG TCATGCTGAA CCACCTCTAT 
GCACTCTCTA TCAAGGATGG AGTGATGGTG CTCAGTGCGA CCCATCGGTA CAAGAAAAAG 
TACGTCACCA CCCTCCTCTA CAAGCCCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkab1 ( NM_031976.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_114182.1 Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 1 (Prkab1), mRNA.

Price & Availability↑

CloneID	ORa08890
Clone ID Related Accession (Same CDS sequence)	NM_031976.1
Accession Version	NM_031976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	5'-AMP-activated protein kinase subunit beta-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U42411.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U42411.1, BC062008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAATA CGAGCAGCGA GCGCGCCGCG CTGGAGCGGC AGGCTGGCCA TAAGACGCCG 
CGGAGGGACA GCTCGGAGGG CACCAAGGAT GGGGACAGGC CCAAGATCCT GATGGACAGC 
CCCGAAGACG CCGACATCTT CCACACCGAG GAAATGAAGG CTCCAGAGAA GGAGGAGTTC 
CTGGCGTGGC AGCACGACCT CGAGGTGAAT GAGAAAGCCC CCGCCCAGGC TCGGCCCACC 
GTATTTCGAT GGACAGGGGG TGGAAAGGAG GTCTACTTGT CTGGATCCTT CAACAACTGG 
AGCAAATTGC CCCTCACTAG AAGCCAAAAC AACTTCGTAG CCATCCTGGA CCTNCCGGAA 
GGAGAGCATC AGTACAAGTT CTTTGTGGAT GGCCAGTGGA CCCACGATCC TTCCGAGCCA 
ATAGTAACCA GCCAGCTTGG CACAGTTAAC AACATCATTC AAGTGAAGAA AACTGACTTT 
GAAGTATTTG ATGCTTTAAT GGTGGATTCC CAAAAGTGCT CCGATGTATC TGAGCTGTCC 
AGTTCCCCCC CAGGACCCTA CCACCAGGAG CCTTACATCT CTAAACCAGA GGAGCGGTTC 
AAGGCCCCGC CCATCCTCCC GCCTCACCTG CTGCAGGTCA TCTTGAACAA GGACACGGGC 
ATCTCTTGTG ATCCAGCGCT GCTTCCGGAG CCCAACCACG TCATGCTGAA CCACCTCTAT 
GCACTCTCTA TCAAGGATGG AGTGATGGTG CTCAGTGCGA CCCATCGGTA CAAGAAAAAG 
TACGTCACCA CCCTCCTCTA CAAGCCCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114182.1
CDS	128..940
Translation	MGNTSSERAALERQAGHKTPRRDSSEGTKDGDRPKILMDSPEDADIFHTEEMKAPEKEEFLAWQHDLEVNEKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSQNNFVAILDnullPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYISKPEERFKAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI

Target ORF information:

RefSeq Version	NM_031976.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 1 (Prkab1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031976.1

ORF Insert Sequence:

ATGGGCAATA CGAGCAGCGA GCGCGCCGCG CTGGAGCGGC AGGCTGGCCA TAAGACGCCG 
CGGAGGGACA GCTCGGAGGG CACCAAGGAT GGGGACAGGC CCAAGATCCT GATGGACAGC 
CCCGAAGACG CCGACATCTT CCACACCGAG GAAATGAAGG CTCCAGAGAA GGAGGAGTTC 
CTGGCGTGGC AGCACGACCT CGAGGTGAAT GAGAAAGCCC CCGCCCAGGC TCGGCCCACC 
GTATTTCGAT GGACAGGGGG TGGAAAGGAG GTCTACTTGT CTGGATCCTT CAACAACTGG 
AGCAAATTGC CCCTCACTAG AAGCCAAAAC AACTTCGTAG CCATCCTGGA CCTNCCGGAA 
GGAGAGCATC AGTACAAGTT CTTTGTGGAT GGCCAGTGGA CCCACGATCC TTCCGAGCCA 
ATAGTAACCA GCCAGCTTGG CACAGTTAAC AACATCATTC AAGTGAAGAA AACTGACTTT 
GAAGTATTTG ATGCTTTAAT GGTGGATTCC CAAAAGTGCT CCGATGTATC TGAGCTGTCC 
AGTTCCCCCC CAGGACCCTA CCACCAGGAG CCTTACATCT CTAAACCAGA GGAGCGGTTC 
AAGGCCCCGC CCATCCTCCC GCCTCACCTG CTGCAGGTCA TCTTGAACAA GGACACGGGC 
ATCTCTTGTG ATCCAGCGCT GCTTCCGGAG CCCAACCACG TCATGCTGAA CCACCTCTAT 
GCACTCTCTA TCAAGGATGG AGTGATGGTG CTCAGTGCGA CCCATCGGTA CAAGAAAAAG 
TACGTCACCA CCCTCCTCTA CAAGCCCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lack of Adipocyte AMPK Exacerbates Insulin Resistance and Hepatic Steatosis through Brown and Beige Adipose Tissue Function.
Cell metabolism24(1)118-29(2016 Jul)
Mottillo EP,Desjardins EM,Crane JD,Smith BK,Green AE,Ducommun S,Henriksen TI,Rebalka IA,Razi A,Sakamoto K,Scheele C,Kemp BE,Hawke TJ,Ortega J,Granneman JG,Steinberg GR

Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.
PLoS genetics10(10)e1004705(2014 Oct)
DiTommaso T,Jones LK,Cottle DL,,Gerdin AK,Vancollie VE,Watt FM,Ramirez-Solis R,Bradley A,Steel KP,Sundberg JP,White JK,Smyth IM

Structural insights into the architecture and allostery of full-length AMP-activated protein kinase.
Structure (London, England : 1993)19(4)515-22(2011 Apr)
Zhu L,Chen L,Zhou XM,Zhang YY,Zhang YJ,Zhao J,Ji SR,Wu JW,Wu Y

Modulation of the activities of AMP-activated protein kinase, protein kinase B, and mammalian target of rapamycin by limiting energy availability with 2-deoxyglucose.
Molecular carcinogenesis47(8)616-28(2008 Aug)
Jiang W,Zhu Z,Thompson HJ

Activation of 5'-AMP-activated kinase with diabetes drug metformin induces casein kinase Iepsilon (CKIepsilon)-dependent degradation of clock protein mPer2.
The Journal of biological chemistry282(29)20794-8(2007 Jul)
Um JH,Yang S,Yamazaki S,Kang H,Viollet B,Foretz M,Chung JH

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Prkab1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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