Vamp8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vamp8
Entrez Gene ID	83730
Full Name	vesicle-associated membrane protein 8
General protein information	Preferred Names vesicle-associated membrane protein 8 Names vesicle-associated membrane protein 8 EDB VAMP-8 endobrevin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q31
Summary	may mediate exocytosis of synaptic vesicles in neurons [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031827.1	NP_114015.1	vesicle-associated membrane protein 8

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	VAMP8	XP_003309126.1
Canis lupus familiaris (dog)	VAMP8	XP_852312.1
Mus musculus (house mouse)	Vamp8	NP_058074.2
Homo sapiens (human)	VAMP8	NP_003752.2
Rattus norvegicus (Norway rat)	Vamp8	NP_114015.1
	VAMP8	XP_001086599.1
Bos taurus (cattle)	VAMP8	NP_001029462.1
Danio rerio (zebrafish)	vamp8	NP_956053.1
Xenopus tropicalis (tropical clawed frog)	vamp8	NP_001016285.2

Gene Ontology
Bibliography

Related articles in PubMed

Acute acinar pancreatitis blocks vesicle-associated membrane protein 8 (VAMP8)-dependent secretion, resulting in intracellular trypsin accumulation.
Messenger SW, Jones EK, Holthaus CL, Thomas DDH, Cooley MM, Byrne JA, Mareninova OA, Gukovskaya AS, Groblewski GE
The Journal of biological chemistry292(19)7828-7839(2017 05)

LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes.
Hubert V, Peschel A, Langer B, Gr?ger M, Rees A, Kain R
Biology open5(10)1516-1529(2016 Oct)

Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion.
Malmersj? S, Di Palma S, Diao J, Lai Y, Pfuetzner RA, Wang AL, McMahon MA, Hayer A, Porteus M, Bodenmiller B, Brunger AT, Meyer T
The EMBO journal35(16)1810-21(2016 08)

ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes.
Diao J, Liu R, Rong Y, Zhao M, Zhang J, Lai Y, Zhou Q, Wilz LM, Li J, Vivona S, Pfuetzner RA, Brunger AT, Zhong Q
Nature520(7548)563-6(2015 Apr)

UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.
Pirooz SD, He S, Zhang T, Zhang X, Zhao Z, Oh S, O'Connell D, Khalilzadeh P, Amini-Bavil-Olyaee S, Farzan M, Liang C
Proceedings of the National Academy of Sciences of the United States of America111(7)2716-21(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

acute inhibition of VAMP8-mediated secretion during pancreatitis triggers intracellular trypsin accumulation and loss of the early endosomal compartment
Title: Acute acinar pancreatitis blocks vesicle-associated membrane protein 8 (VAMP8)-dependent secretion, resulting in intracellular trypsin accumulation.

The authors took a proteomics approach using a PKCB knockout mast cell model and found that the key mast cell secretory protein VAMP8 becomes phosphorylated by PKC at multiple residues in the SNARE domain.
Title: Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion.

VAMP8, which also mediates fusion of late endosomes and lysosomes, plays a major role in hexosaminidase release
Title: Endobrevin/VAMP8 mediates exocytotic release of hexosaminidase from rat basophilic leukaemia cells.

Individual pancreatic acinar cells express VAMP 8-specific populations of zymogen granules that orchestrate the constitutive and calcium(2+)-regulated secretory pathways.
Title: Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.

Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs
Title: Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs.

The following Vamp8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vamp8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08828	NM_031827.1 Latest version!	Rattus norvegicus vesicle-associated membrane protein 8 (Vamp8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vamp8 ( NM_031827.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_114015.1 Rattus norvegicus vesicle-associated membrane protein 8 (Vamp8), mRNA.

Price & Availability↑

CloneID	ORa08828
Clone ID Related Accession (Same CDS sequence)	NM_031827.1
Accession Version	NM_031827.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	vesicle-associated membrane protein 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF132812.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF132812.1, CF249537.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCCA GTGGGAGTGC CGGAAATGAC CGAGTCAGGA ACCTGCAGAG TGAGGTGGAG 
GGAGTCAAGA ATATTATGAC CCAGAATGTG GAGCGGATCT TGGCCCGAGG GGAGAACCTG 
GACCATCTCC GAAACAAGAC AGAGGACTTG GAAGCCACGT CTGAACACTT CAAGACGACG 
TCCCAGAAGG TGGCCCGGAA GTTCTGGTGG AAGAATGTGA AGATGATTGT CATCATCTGT 
GTGATTGTCC TTATCATTCT CATCCTCATC ATACTCTTTG CTACGGGCAC CATCCCCACT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114015.1
CDS	1..303
Translation	MEASGSAGNDRVRNLQSEVEGVKNIMTQNVERILARGENLDHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVIICVIVLIILILIILFATGTIPT

Target ORF information:

RefSeq Version	NM_031827.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus vesicle-associated membrane protein 8 (Vamp8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031827.1

ORF Insert Sequence:

ATGGAGGCCA GTGGGAGTGC CGGAAATGAC CGAGTCAGGA ACCTGCAGAG TGAGGTGGAG 
GGAGTCAAGA ATATTATGAC CCAGAATGTG GAGCGGATCT TGGCCCGAGG GGAGAACCTG 
GACCATCTCC GAAACAAGAC AGAGGACTTG GAAGCCACGT CTGAACACTT CAAGACGACG 
TCCCAGAAGG TGGCCCGGAA GTTCTGGTGG AAGAATGTGA AGATGATTGT CATCATCTGT 
GTGATTGTCC TTATCATTCT CATCCTCATC ATACTCTTTG CTACGGGCAC CATCCCCACT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Acute acinar pancreatitis blocks vesicle-associated membrane protein 8 (VAMP8)-dependent secretion, resulting in intracellular trypsin accumulation.
The Journal of biological chemistry292(19)7828-7839(2017 05)
Messenger SW,Jones EK,Holthaus CL,Thomas DDH,Cooley MM,Byrne JA,Mareninova OA,Gukovskaya AS,Groblewski GE

LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes.
Biology open5(10)1516-1529(2016 Oct)
Hubert V,Peschel A,Langer B,Gr?ger M,Rees A,Kain R

Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion.
The EMBO journal35(16)1810-21(2016 08)
Malmersj? S,Di Palma S,Diao J,Lai Y,Pfuetzner RA,Wang AL,McMahon MA,Hayer A,Porteus M,Bodenmiller B,Brunger AT,Meyer T

ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes.
Nature520(7548)563-6(2015 Apr)
Diao J,Liu R,Rong Y,Zhao M,Zhang J,Lai Y,Zhou Q,Wilz LM,Li J,Vivona S,Pfuetzner RA,Brunger AT,Zhong Q

UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.
Proceedings of the National Academy of Sciences of the United States of America111(7)2716-21(2014 Feb)
Pirooz SD,He S,Zhang T,Zhang X,Zhao Z,Oh S,O'Connell D,Khalilzadeh P,Amini-Bavil-Olyaee S,Farzan M,Liang C

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Vamp8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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