Cish cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cish
Entrez Gene ID	83681
Full Name	cytokine inducible SH2-containing protein
Synonyms	Cis
General protein information	Preferred Names cytokine inducible SH2-containing protein Names cytokine-inducible SH2-containing protein CIS-1 SOCS cytokine inducible SH2-containing protein 1 suppressor of cytokine signaling
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	mouse homolog is a negative regulator of cytokine signaling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031804.1	NP_113992.1	cytokine-inducible SH2-containing protein

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CISH	NP_989957.1
Pan troglodytes (chimpanzee)	CISH	XP_003309858.1
Mus musculus (house mouse)	Cish	NP_034025.1
Canis lupus familiaris (dog)	CISH	XP_541873.3
Rattus norvegicus (Norway rat)	Cish	NP_113992.1
Danio rerio (zebrafish)	cishb	NP_001108026.1
Xenopus tropicalis (tropical clawed frog)	cish	NP_001107161.1
Danio rerio (zebrafish)	cish	NP_001070085.1
Homo sapiens (human)	CISH	NP_659508.1
	CISH	XP_001097824.1
Bos taurus (cattle)	CISH	NP_001040051.1

Gene Ontology
Bibliography

Related articles in PubMed

Mechanisms underlying the diminished sensitivity to prolactin negative feedback during lactation: reduced STAT5 signaling and up-regulation of cytokine-inducible SH2 domain-containing protein (CIS) expression in tuberoinfundibular dopaminergic neurons.
Anderson ST, Barclay JL, Fanning KJ, Kusters DH, Waters MJ, Curlewis JD
Endocrinology147(3)1195-202(2006 Mar)

Role of the cytokine-induced SH2 domain-containing protein CIS in growth hormone receptor internalization.
Landsman T, Waxman DJ
The Journal of biological chemistry280(45)37471-80(2005 Nov)

Thrombin induces expression of cytokine-induced SH2 protein (CIS) in rat brain astrocytes: involvement of phospholipase A2, cyclooxygenase, and lipoxygenase.
Ji KA, Yang MS, Jou I, Shong MH, Joe EH
Glia48(2)102-11(2004 Nov)

Human growth hormone induces SOCS3 and CIS mRNA increase in the hypothalamic neurons of hypophysectomized rats.
Kasagi Y, Tokita R, Nakata T, Imaki T, Minami S
Endocrine journal51(2)145-54(2004 Apr)

Functional association of cytokine-induced SH2 protein and protein kinase C in activated T cells.
Chen S, Anderson PO, Li L, Sj?gren HO, Wang P, Li SL
International immunology15(3)403-9(2003 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

during lactation, STAT5 signaling is reduced and CIS is upregulated in tuberoinfundibular dopaminergic neurons
Title: Mechanisms underlying the diminished sensitivity to prolactin negative feedback during lactation: reduced STAT5 signaling and up-regulation of cytokine-inducible SH2 domain-containing protein (CIS) expression in tuberoinfundibular dopaminergic neurons.

The growth hormone(GH)-responsive rat liver cell line CWSV-1 was used to investigate the role of CIS and the proteasome in GH-induced GHR internalization.
Title: Role of the cytokine-induced SH2 domain-containing protein CIS in growth hormone receptor internalization.

Thrombin-stimulation of reactive oxygen species, prostaglandin and leukotriene production in astrocytes results in cytokine-induced SH2 protein [CIS] expression which may be a feedback mechanism preventing prolonged inflammatory responses
Title: Thrombin induces expression of cytokine-induced SH2 protein (CIS) in rat brain astrocytes: involvement of phospholipase A2, cyclooxygenase, and lipoxygenase.

HGH acts directly on the neurons in the hypothalamus, and increases SOCS3 and CIS mRNAs, suggesting that these negative regulators may be involved in the mechanism that turns off the hGH action in the hypothalamic neurons.
Title: Human growth hormone induces SOCS3 and CIS mRNA increase in the hypothalamic neurons of hypophysectomized rats.

inhibition of GH-induced expression in osteoblast-like cells
Title: 1Alpha,25-dihydroxyvitamin D3 inhibits GH-induced expression of SOCS-3 and CIS and prolongs growth hormone signaling via the Janus kinase (JAK2)/signal transducers and activators of transcription (STAT5) system in osteoblast-like cells.

The following Cish gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cish cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08774	NM_031804.1 Latest version!	Rattus norvegicus cytokine inducible SH2-containing protein (Cish), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cish ( NM_031804.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113992.1 Rattus norvegicus cytokine inducible SH2-containing protein (Cish), mRNA.

Price & Availability↑

CloneID	ORa08774
Clone ID Related Accession (Same CDS sequence)	NM_031804.1
Accession Version	NM_031804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	cytokine-inducible SH2-containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161930.1. On or before Sep 7, 2008 this sequence version replaced XM_001074104.1, XM_343472.3. ##Evidence-Data-START## Transcript exon combination :: BC161930.1, AF065161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTCCTCT GCGTACAGGG ATCTTGTCCT TTGCTGGTTG TGGAGCAAAT TGGGCAGCGG 
CCTCTGTGGG CCCAGTCCCT GGAGCTGCCC GGGCCAGCCA TGCAGCCCTT ACCCACTGGG 
GCATTCCCAG AGGAGGTGAC AGAGGAGACC CCTGTCCAGT CAGAGAATGA ACCGAAGGTG 
CTAGACCCTG AGGGAGATCT GCTGTGCATA GCCAAGACGT TCTCCTACCT CCGGGAATCT 
GGTTGGTACT GGGGTTCCAT TACAGCCAGT GAGGCCCGGC AGCATCTACA GAAGATGCCG 
GAGGGCACAT TCCTCGTGCG AGACAGCACC CACCCCAGCT ACCTGTTTAC ATTGTCAGTC 
AAAACCACCC GTGGCCCCAC CAATGTACGC ATCGAGTATG CCGACTCTAG CTTCCGACTG 
GACTCCAATT GCTTGTCGAG ACCTCGAATC CTGGCCTTCC CAGATGTGGT CAGCCTTGTG 
CAGCATTACG TGGCCTCCTG TACGGCTGAC ACCCGGAGTG ACAGCCCAGA TCCTGCTCCC 
ACCCCAGCCC TGCCTGTGCC GAAGCCAGAT GCACCTGGTG ACCCAGTACT GCCTATCCCC 
GTGGCTACTG CTGTGCACCT GAAATTGGTG CAGCCCTTTG TGCGCAGGAG CAGCGCCCGC 
AGCTTACAAC ACCTGTGTCG GCTAGTCATC AACCGACTGG TGACCGATGT GGACTGCCTA 
CCCCTGCCCC GGCGCATGGC CGACTACCTC CGACAGTACC CGTTCCAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113992.1
CDS	116..889
Translation	MVLCVQGSCPLLVVEQIGQRPLWAQSLELPGPAMQPLPTGAFPEEVTEETPVQSENEPKVLDPEGDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRLDSNCLSRPRILAFPDVVSLVQHYVASCTADTRSDSPDPAPTPALPVPKPDAPGDPVLPIPVATAVHLKLVQPFVRRSSARSLQHLCRLVINRLVTDVDCLPLPRRMADYLRQYPFQL

Target ORF information:

RefSeq Version	NM_031804.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytokine inducible SH2-containing protein (Cish), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031804.1

ORF Insert Sequence:

ATGGTCCTCT GCGTACAGGG ATCTTGTCCT TTGCTGGTTG TGGAGCAAAT TGGGCAGCGG 
CCTCTGTGGG CCCAGTCCCT GGAGCTGCCC GGGCCAGCCA TGCAGCCCTT ACCCACTGGG 
GCATTCCCAG AGGAGGTGAC AGAGGAGACC CCTGTCCAGT CAGAGAATGA ACCGAAGGTG 
CTAGACCCTG AGGGAGATCT GCTGTGCATA GCCAAGACGT TCTCCTACCT CCGGGAATCT 
GGTTGGTACT GGGGTTCCAT TACAGCCAGT GAGGCCCGGC AGCATCTACA GAAGATGCCG 
GAGGGCACAT TCCTCGTGCG AGACAGCACC CACCCCAGCT ACCTGTTTAC ATTGTCAGTC 
AAAACCACCC GTGGCCCCAC CAATGTACGC ATCGAGTATG CCGACTCTAG CTTCCGACTG 
GACTCCAATT GCTTGTCGAG ACCTCGAATC CTGGCCTTCC CAGATGTGGT CAGCCTTGTG 
CAGCATTACG TGGCCTCCTG TACGGCTGAC ACCCGGAGTG ACAGCCCAGA TCCTGCTCCC 
ACCCCAGCCC TGCCTGTGCC GAAGCCAGAT GCACCTGGTG ACCCAGTACT GCCTATCCCC 
GTGGCTACTG CTGTGCACCT GAAATTGGTG CAGCCCTTTG TGCGCAGGAG CAGCGCCCGC 
AGCTTACAAC ACCTGTGTCG GCTAGTCATC AACCGACTGG TGACCGATGT GGACTGCCTA 
CCCCTGCCCC GGCGCATGGC CGACTACCTC CGACAGTACC CGTTCCAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanisms underlying the diminished sensitivity to prolactin negative feedback during lactation: reduced STAT5 signaling and up-regulation of cytokine-inducible SH2 domain-containing protein (CIS) expression in tuberoinfundibular dopaminergic neurons.
Endocrinology147(3)1195-202(2006 Mar)
Anderson ST,Barclay JL,Fanning KJ,Kusters DH,Waters MJ,Curlewis JD

Role of the cytokine-induced SH2 domain-containing protein CIS in growth hormone receptor internalization.
The Journal of biological chemistry280(45)37471-80(2005 Nov)
Landsman T,Waxman DJ

Thrombin induces expression of cytokine-induced SH2 protein (CIS) in rat brain astrocytes: involvement of phospholipase A2, cyclooxygenase, and lipoxygenase.
Glia48(2)102-11(2004 Nov)
Ji KA,Yang MS,Jou I,Shong MH,Joe EH

Human growth hormone induces SOCS3 and CIS mRNA increase in the hypothalamic neurons of hypophysectomized rats.
Endocrine journal51(2)145-54(2004 Apr)
Kasagi Y,Tokita R,Nakata T,Imaki T,Minami S

Functional association of cytokine-induced SH2 protein and protein kinase C in activated T cells.
International immunology15(3)403-9(2003 Mar)
Chen S,Anderson PO,Li L,Sj?gren HO,Wang P,Li SL

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Cish cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報