Deaf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Deaf1
Entrez Gene ID	83632
Full Name	DEAF1 transcription factor
Synonyms	NUDR
General protein information	Preferred Names DEAF1 transcription factor Names deformed epidermal autoregulatory factor 1 homolog DEAF-1 related transcriptional regulator (NUDR) nuclear DEAF-1-related transcriptional regulator suppressin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q41
Summary	acts as a transcriptional activator; may regulate transcription during fetal development or testicular cell differentiation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008760014.2	XP_008758236.1	deformed epidermal autoregulatory factor 1 homolog isoform X4
XM_006230582.3	XP_006230644.1	deformed epidermal autoregulatory factor 1 homolog isoform X5
XM_006230583.3	XP_006230645.1	deformed epidermal autoregulatory factor 1 homolog isoform X6
XM_008760012.2	XP_008758234.1	deformed epidermal autoregulatory factor 1 homolog isoform X2
NM_031801.2	NP_113989.2	deformed epidermal autoregulatory factor 1 homolog
XM_008760011.2	XP_008758233.1	deformed epidermal autoregulatory factor 1 homolog isoform X1
XM_008760013.2	XP_008758235.1	deformed epidermal autoregulatory factor 1 homolog isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	DEAF1	NP_066288.2
Canis lupus familiaris (dog)	DEAF1	XP_540529.2
Rattus norvegicus (Norway rat)	Deaf1	NP_113989.1
Gallus gallus (chicken)	DEAF1	NP_001131121.1
Danio rerio (zebrafish)	zgc:194895	NP_001124251.1
Bos taurus (cattle)	DEAF1	XP_002699457.2
Mus musculus (house mouse)	Deaf1	NP_058570.1
	DEAF1	XP_001116691.2
Pan troglodytes (chimpanzee)	DEAF1	XP_003951841.1
Xenopus tropicalis (tropical clawed frog)	deaf1	XP_004913722.1

Gene Ontology
Bibliography

Related articles in PubMed

Stress-induced alterations in 5-HT1A receptor transcriptional modulators NUDR and Freud-1.
Szewczyk B, Kotarska K, Daigle M, Misztak P, Sowa-Kucma M, Rafalo A, Curzytek K, Kubera M, Basta-Kaim A, Nowak G, Albert PR
The international journal of neuropsychopharmacology17(11)1763-75(2014 Nov)

Mutations affecting the SAND domain of DEAF1 cause intellectual disability with severe speech impairment and behavioral problems.
Vulto-van Silfhout AT, Rajamanickam S, Jensik PJ, Vergult S, de Rocker N, Newhall KJ, Raghavan R, Reardon SN, Jarrett K, McIntyre T, Bulinski J, Ownby SL, Huggenvik JI, McKnight GS, Rose GM, Cai X, Willaert A, Zweier C, Endele S, de Ligt J, van Bon BW, Lugtenberg D, de Vries PF, Veltman JA, van Bokhoven H, Brunner HG, Rauch A, de Brouwer AP, Carvill GL, Hoischen A, Mefford HC, Eichler EE, Vissers LE, Menten B, Collard MW, de Vries BB
American journal of human genetics94(5)649-61(2014 May)

Chronic social defeat downregulates the 5-HT1A receptor but not Freud-1 or NUDR in the rat prefrontal cortex.
Kieran N, Ou XM, Iyo AH
Neuroscience letters469(3)380-4(2010 Jan)

Differential regulation of the serotonin 1 A transcriptional modulators five prime repressor element under dual repression-1 and nuclear-deformed epidermal autoregulatory factor by chronic stress.
Iyo AH, Kieran N, Chandran A, Albert PR, Wicks I, Bissette G, Austin MC
Neuroscience163(4)1119-27(2009 Nov)

Deaf1 isoforms control the expression of genes encoding peripheral tissue antigens in the pancreatic lymph nodes during type 1 diabetes.
Yip L, Su L, Sheng D, Chang P, Atkinson M, Czesak M, Albert PR, Collier AR, Turley SJ, Fathman CG, Creusot RJ
Nature immunology10(9)1026-33(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In pregnancy stress, prefrontal cortex Deaf1 levels were reduced in rats.
Title: Stress-induced alterations in 5-HT1A receptor transcriptional modulators NUDR and Freud-1.

chronic social defeat stress downregulates the 5-HT1A receptor mRNA but does not significantly alter the levels of Freud-1 and NUDR in PFC; this stress paradigm could offer a useful rodent model for depression and other stress related disorders
Title: Chronic social defeat downregulates the 5-HT1A receptor but not Freud-1 or NUDR in the rat prefrontal cortex.

results point to the importance of understanding the mechanism for the differential regulation of 5-HT1AR, Freud-1 and NUDR in the prefrontal cortex as a basis for understanding the related effects of chronic stress and stress-related disorders
Title: Differential regulation of the serotonin 1 A transcriptional modulators five prime repressor element under dual repression-1 and nuclear-deformed epidermal autoregulatory factor by chronic stress.

The following Deaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Deaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa23625	XM_006230582.3 Latest version!	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa23626	XM_006230583.3 Latest version!	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa43774	XM_008760014.2 Latest version!	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa43777	NM_031801.2 Latest version!	Rattus norvegicus DEAF1 transcription factor (Deaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa43771	XM_008760011.2 Latest version!	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa43772	XM_008760012.2 Latest version!	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa43773	XM_008760013.2 Latest version!	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Deaf1 ( XM_006230582.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230644.1 Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa23625
Clone ID Related Accession (Same CDS sequence)	XM_006230582.3
Accession Version	XM_006230582.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	deformed epidermal autoregulatory factor 1 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG CAGCTTCTTT GCAAAGGGAG AAAGAAGTGA CCACAGTGAC TGTGGCCAAC 
GTGGGGTCCT CTGCAGATAA CGTCTTCACA ACATCAGTGG CAAATGCAGC CTCGATATCA 
GGACATGTCC TGTCTGGTAG GACAGCCCTG CAGATCGGGG ACAGCCTGAA CACTGAGAAA 
GCCACACTAA TAGTTGTACA CACAGATGGG AGCATTGTGG AAACCACCGG GCTGAAGGGC 
CCAGCAGCAC CTCTCACCCC AGGTCCTCAG TCTCCTCCTA CCCCACTGGC ACCTGGCCAG 
GAGAAAGGTG GTACCAAGTA CAACTGGGAC CCTTCGGTGT ATGACAGCGA GCTGCCTGTG 
CGCTGTCGGA ACATCAGTGG CACGCTCTAC AAGAGTCGGC TCGGCTCAGG TGGCCGGGGG 
CGGTGTATCA AGCAGGGAGA AAACTGGTAC AGCCCAACCG AGTTTGAAGC CATGGCAGGA 
AGAGCCAGCA GCAAGGACTG GAAGAGAAGC ATCCGCTATG CGGGCAGACC CCTGCAGTGC 
CTCATCCAGG ATGGTATTTT GAACCCTCAC GCTGCCTCCT GCACCTGTGC TGCCTGTTGC 
GATGACATGA CTCTAAGTGG CCCTGTCAGG CTCTTCGTCC CCTATAAAAG GCGCAAGAAA 
GAGAATGAGC TGCCCACGAC TCCAGTTAAG AAGGATTCCC CCAAGAACAT CACGCTGCTC 
CCTGCCACGG CGGCCACCAC CTTCACTGTG ACACCCTCAG GACAGATCAC TACCTCTGGA 
GCACTAACCT TTGACCGAGC ATCCACTGTG GAGGCCACTG CTGTCATCTC TGAGAGCCCA 
GCCCAAGGGG ATGTCTTTGC AGGAGCCACA GTGCAAGAGG CAGGCGTGCA GCCCCCCTGC 
AGGGTTGGCC ACCCTGAACC CCACTACCCT GGCTATCAGG ACAGCTGCCA GATCGCCCCG 
TTTCCAGAAG CTGCATTGCC AACATCACAT CCCAAAATTG TCCTGACATC GCTGCCCGCC 
TTGGCCGTGC CGCCCTCCAC CCCCACCAAA GCTGTCTCTC CCACCGTGGT CAGTGGGCTG 
GAGATGTCAG AGCATCGGAG CTGGCTGTAC CTGGAAGAGA TGGTCAACTC CCTCCTCAAC 
ACAGCCCAGC AGCTGAAGAC GTTGTTTGAA CAAGCCAAGC AGGCGAGCTC TTGCAGGGAA 
GCTGCTGTGA CCCAGGCGAG AATGCAGGTT GATGCAGAGA GGAAGGAGCA GTCATGTGTC 
AACTGCGGCC GGGAGGCCAT GAGTGAGTGC ACCGGCTGCC ACAAGGTTAA CTACTGCTCT 
ACGTTCTGCC AGCGCAAGGA CTGGAAAGAC CATCAGCATG TATGTGGCCA GTCAGCATCT 
GTCACTGTCC AGGCTGATGA CGTCCATGTT GAAGAAAGTG TGATAGAAAA AGTTACTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230644.1
CDS	1229..2671
Translation	MGLAASLQREKEVTTVTVANVGSSADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKSRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGATVQEAGVQPPCRVGHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPSTPTKAVSPTVVSGLEMSEHRSWLYLEEMVNSLLNTAQQLKTLFEQAKQASSCREAAVTQARMQVDAERKEQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHVCGQSASVTVQADDVHVEESVIEKVTV

Target ORF information:

RefSeq Version	XM_006230582.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230582.3

ORF Insert Sequence:

ATGGGACTTG CAGCTTCTTT GCAAAGGGAG AAAGAAGTGA CCACAGTGAC TGTGGCCAAC 
GTGGGGTCCT CTGCAGATAA CGTCTTCACA ACATCAGTGG CAAATGCAGC CTCGATATCA 
GGACATGTCC TGTCTGGTAG GACAGCCCTG CAGATCGGGG ACAGCCTGAA CACTGAGAAA 
GCCACACTAA TAGTTGTACA CACAGATGGG AGCATTGTGG AAACCACCGG GCTGAAGGGC 
CCAGCAGCAC CTCTCACCCC AGGTCCTCAG TCTCCTCCTA CCCCACTGGC ACCTGGCCAG 
GAGAAAGGTG GTACCAAGTA CAACTGGGAC CCTTCGGTGT ATGACAGCGA GCTGCCTGTG 
CGCTGTCGGA ACATCAGTGG CACGCTCTAC AAGAGTCGGC TCGGCTCAGG TGGCCGGGGG 
CGGTGTATCA AGCAGGGAGA AAACTGGTAC AGCCCAACCG AGTTTGAAGC CATGGCAGGA 
AGAGCCAGCA GCAAGGACTG GAAGAGAAGC ATCCGCTATG CGGGCAGACC CCTGCAGTGC 
CTCATCCAGG ATGGTATTTT GAACCCTCAC GCTGCCTCCT GCACCTGTGC TGCCTGTTGC 
GATGACATGA CTCTAAGTGG CCCTGTCAGG CTCTTCGTCC CCTATAAAAG GCGCAAGAAA 
GAGAATGAGC TGCCCACGAC TCCAGTTAAG AAGGATTCCC CCAAGAACAT CACGCTGCTC 
CCTGCCACGG CGGCCACCAC CTTCACTGTG ACACCCTCAG GACAGATCAC TACCTCTGGA 
GCACTAACCT TTGACCGAGC ATCCACTGTG GAGGCCACTG CTGTCATCTC TGAGAGCCCA 
GCCCAAGGGG ATGTCTTTGC AGGAGCCACA GTGCAAGAGG CAGGCGTGCA GCCCCCCTGC 
AGGGTTGGCC ACCCTGAACC CCACTACCCT GGCTATCAGG ACAGCTGCCA GATCGCCCCG 
TTTCCAGAAG CTGCATTGCC AACATCACAT CCCAAAATTG TCCTGACATC GCTGCCCGCC 
TTGGCCGTGC CGCCCTCCAC CCCCACCAAA GCTGTCTCTC CCACCGTGGT CAGTGGGCTG 
GAGATGTCAG AGCATCGGAG CTGGCTGTAC CTGGAAGAGA TGGTCAACTC CCTCCTCAAC 
ACAGCCCAGC AGCTGAAGAC GTTGTTTGAA CAAGCCAAGC AGGCGAGCTC TTGCAGGGAA 
GCTGCTGTGA CCCAGGCGAG AATGCAGGTT GATGCAGAGA GGAAGGAGCA GTCATGTGTC 
AACTGCGGCC GGGAGGCCAT GAGTGAGTGC ACCGGCTGCC ACAAGGTTAA CTACTGCTCT 
ACGTTCTGCC AGCGCAAGGA CTGGAAAGAC CATCAGCATG TATGTGGCCA GTCAGCATCT 
GTCACTGTCC AGGCTGATGA CGTCCATGTT GAAGAAAGTG TGATAGAAAA AGTTACTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Deaf1 ( XM_006230583.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230645.1 Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa23626
Clone ID Related Accession (Same CDS sequence)	XM_006230583.3
Accession Version	XM_006230583.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	deformed epidermal autoregulatory factor 1 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG CAGCTTCTTT GCAAAGGGAG AAAGAAGTGA CCACAGTGAC TGTGGCCAAC 
GTGGGGTCCT CTGCAGATAA CGTCTTCACA ACATCAGTGG CAAATGCAGC CTCGATATCA 
GGACATGTCC TGTCTGGTAG GACAGCCCTG CAGATCGGGG ACAGCCTGAA CACTGAGAAA 
GCCACACTAA TAGTTGTACA CACAGATGGG AGCATTGTGG AAACCACCGG GCTGAAGGGC 
CCAGCAGCAC CTCTCACCCC AGGTCCTCAG TCTCCTCCTA CCCCACTGGC ACCTGGCCAG 
GAGAAAGGTG GTACCAAGTA CAACTGGGAC CCTTCGGTGT ATGACAGCGA GCTGCCTGTG 
CGCTGTCGGA ACATCAGTGG CACGCTCTAC AAGAGTCGGC TCGGCTCAGG TGGCCGGGGG 
CGGTGTATCA AGCAGGGAGA AAACTGGTAC AGCCCAACCG AGTTTGAAGC CATGGCAGGA 
AGAGCCAGCA GCAAGGACTG GAAGAGAAGC ATCCGCTATG CGGGCAGACC CCTGCAGTGC 
CTCATCCAGG ATGGTATTTT GAACCCTCAC GCTGCCTCCT GCACCTGTGC TGCCTGTTGC 
GATGACATGA CTCTAAGTGG CCCTGTCAGG CTCTTCGTCC CCTATAAAAG GCGCAAGAAA 
GAGAATGAGC TGCCCACGAC TCCAGTTAAG AAGGATTCCC CCAAGAACAT CACGCTGCTC 
CCTGCCACGG CGGCCACCAC CTTCACTGTG ACACCCTCAG GACAGATCAC TACCTCTGGA 
GCACTAACCT TTGACCGAGC ATCCACTGTG GAGGCCACTG CTGTCATCTC TGAGAGCCCA 
GCCCAAGGGG ATGTCTTTGC AGGAGCCACA GTGCAAGAGG CAGGCGTGCA GCCCCCCTGC 
AGGGTTGGCC ACCCTGAACC CCACTACCCT GGCTATCAGG ACAGCTGCCA GATCGCCCCG 
TTTCCAGAAG CTGCATTGCC AACATCACAT CCCAAAATTG TCCTGACATC GCTGCCCGCC 
TTGGCCGTGC CGCCCTCCAC CCCCACCAAA GCTGTCTCTC CCACCGTGGT CAGTGGGCTG 
GAGATGTCAG AGCATCGGAG CTGGCTGTAC CTGGAAGAGA TGGTCAACTC CCTCCTCAAC 
ACAGCCCAGC AGCTGAAGAC GTTGTTTGAA CAAGCCAAGC AGGCGAGCTC TTGCAGGGAA 
GCTGCTGTGA CCCAGGCGAG AATGCAGGTT GATGCAGAGA GGAAGGAGGA CTGGAAAGAC 
CATCAGCATG TATGTGGCCA GTCAGCATCT GTCACTGTCC AGGCTGATGA CGTCCATGTT 
GAAGAAAGTG TGATAGAAAA AGTTACTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230645.1
CDS	1229..2581
Translation	MGLAASLQREKEVTTVTVANVGSSADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKSRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGATVQEAGVQPPCRVGHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPSTPTKAVSPTVVSGLEMSEHRSWLYLEEMVNSLLNTAQQLKTLFEQAKQASSCREAAVTQARMQVDAERKEDWKDHQHVCGQSASVTVQADDVHVEESVIEKVTV

Target ORF information:

RefSeq Version	XM_006230583.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230583.3

ORF Insert Sequence:

ATGGGACTTG CAGCTTCTTT GCAAAGGGAG AAAGAAGTGA CCACAGTGAC TGTGGCCAAC 
GTGGGGTCCT CTGCAGATAA CGTCTTCACA ACATCAGTGG CAAATGCAGC CTCGATATCA 
GGACATGTCC TGTCTGGTAG GACAGCCCTG CAGATCGGGG ACAGCCTGAA CACTGAGAAA 
GCCACACTAA TAGTTGTACA CACAGATGGG AGCATTGTGG AAACCACCGG GCTGAAGGGC 
CCAGCAGCAC CTCTCACCCC AGGTCCTCAG TCTCCTCCTA CCCCACTGGC ACCTGGCCAG 
GAGAAAGGTG GTACCAAGTA CAACTGGGAC CCTTCGGTGT ATGACAGCGA GCTGCCTGTG 
CGCTGTCGGA ACATCAGTGG CACGCTCTAC AAGAGTCGGC TCGGCTCAGG TGGCCGGGGG 
CGGTGTATCA AGCAGGGAGA AAACTGGTAC AGCCCAACCG AGTTTGAAGC CATGGCAGGA 
AGAGCCAGCA GCAAGGACTG GAAGAGAAGC ATCCGCTATG CGGGCAGACC CCTGCAGTGC 
CTCATCCAGG ATGGTATTTT GAACCCTCAC GCTGCCTCCT GCACCTGTGC TGCCTGTTGC 
GATGACATGA CTCTAAGTGG CCCTGTCAGG CTCTTCGTCC CCTATAAAAG GCGCAAGAAA 
GAGAATGAGC TGCCCACGAC TCCAGTTAAG AAGGATTCCC CCAAGAACAT CACGCTGCTC 
CCTGCCACGG CGGCCACCAC CTTCACTGTG ACACCCTCAG GACAGATCAC TACCTCTGGA 
GCACTAACCT TTGACCGAGC ATCCACTGTG GAGGCCACTG CTGTCATCTC TGAGAGCCCA 
GCCCAAGGGG ATGTCTTTGC AGGAGCCACA GTGCAAGAGG CAGGCGTGCA GCCCCCCTGC 
AGGGTTGGCC ACCCTGAACC CCACTACCCT GGCTATCAGG ACAGCTGCCA GATCGCCCCG 
TTTCCAGAAG CTGCATTGCC AACATCACAT CCCAAAATTG TCCTGACATC GCTGCCCGCC 
TTGGCCGTGC CGCCCTCCAC CCCCACCAAA GCTGTCTCTC CCACCGTGGT CAGTGGGCTG 
GAGATGTCAG AGCATCGGAG CTGGCTGTAC CTGGAAGAGA TGGTCAACTC CCTCCTCAAC 
ACAGCCCAGC AGCTGAAGAC GTTGTTTGAA CAAGCCAAGC AGGCGAGCTC TTGCAGGGAA 
GCTGCTGTGA CCCAGGCGAG AATGCAGGTT GATGCAGAGA GGAAGGAGGA CTGGAAAGAC 
CATCAGCATG TATGTGGCCA GTCAGCATCT GTCACTGTCC AGGCTGATGA CGTCCATGTT 
GAAGAAAGTG TGATAGAAAA AGTTACTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Deaf1 ( XM_008760014.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758236.1 Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa43774
Clone ID Related Accession (Same CDS sequence)	XM_008760014.2
Accession Version	XM_008760014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	deformed epidermal autoregulatory factor 1 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG CAGCTTCTTT GCAAAGGGAG AAAGAAGTGA CCACAGTGAC TGTGGCCAAC 
GTGGGGTCCT CTGCAGATAA CGTCTTCACA ACATCAGTGG CAAATGCAGC CTCGATATCA 
GGACATGTCC TGTCTGGTAG GACAGCCCTG CAGATCGGGG ACAGCCTGAA CACTGAGAAA 
GCCACACTAA TAGTTGTACA CACAGATGGG AGCATTGTGG AAACCACCGG GCTGAAGGGC 
CCAGCAGCAC CTCTCACCCC AGCCTCTCTT TGCCCAGGTC CTCAGTCTCC TCCTACCCCA 
CTGGCACCTG GCCAGGAGAA AGGTGGTACC AAGTACAACT GGGACCCTTC GGTGTATGAC 
AGCGAGCTGC CTGTGCGCTG TCGGAACATC AGTGGCACGC TCTACAAGAG TCGGCTCGGC 
TCAGGTGGCC GGGGGCGGTG TATCAAGCAG GGAGAAAACT GGTACAGCCC AACCGAGTTT 
GAAGCCATGG CAGGAAGAGC CAGCAGCAAG GACTGGAAGA GAAGCATCCG CTATGCGGGC 
AGACCCCTGC AGTGCCTCAT CCAGGATGGT ATTTTGAACC CTCACGCTGC CTCCTGCACC 
TGTGCTGCCT GTTGCGATGA CATGACTCTA AGTGGCCCTG TCAGGCTCTT CGTCCCCTAT 
AAAAGGCGCA AGAAAGAGAA TGAGCTGCCC ACGACTCCAG TTAAGAAGGA TTCCCCCAAG 
AACATCACGC TGCTCCCTGC CACGGCGGCC ACCACCTTCA CTGTGACACC CTCAGGACAG 
ATCACTACCT CTGGAGCACT AACCTTTGAC CGAGCATCCA CTGTGGAGGC CACTGCTGTC 
ATCTCTGAGA GCCCAGCCCA AGGGGATGTC TTTGCAGGAG CCACAGTGCA AGAGGCAGGC 
GTGCAGCCCC CCTGCAGGGT TGGCCACCCT GAACCCCACT ACCCTGGCTA TCAGGACAGC 
TGCCAGATCG CCCCGTTTCC AGAAGCTGCA TTGCCAACAT CACATCCCAA AATTGTCCTG 
ACATCGCTGC CCGCCTTGGC CGTGCCGCCC TCCACCCCCA CCAAAGCTGT CTCTCCCACC 
GTGGTCAGTG GGCTGGAGAT GTCAGAGCAT CGGAGCTGGC TGTACCTGGA AGAGATGGTC 
AACTCCCTCC TCAACACAGC CCAGCAGCTG AAGACGTTGT TTGAACAAGC CAAGCAGGCG 
AGCTCTTGCA GGGAAGCTGC TGTGACCCAG GCGAGAATGC AGGTTGATGC AGAGAGGAAG 
GAGCAGTCAT GTGTCAACTG CGGCCGGGAG GCCATGAGTG AGTGCACCGG CTGCCACAAG 
GTTAACTACT GCTCTACGTT CTGCCAGCGC AAGGACTGGA AAGACCATCA GCATGTATGT 
GGCCAGTCAG CATCTGTCAC TGTCCAGGCT GATGACGTCC ATGTTGAAGA AAGTGTGATA 
GAAAAAGTTA CTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758236.1
CDS	1230..2687
Translation	MGLAASLQREKEVTTVTVANVGSSADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPASLCPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKSRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGATVQEAGVQPPCRVGHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPSTPTKAVSPTVVSGLEMSEHRSWLYLEEMVNSLLNTAQQLKTLFEQAKQASSCREAAVTQARMQVDAERKEQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHVCGQSASVTVQADDVHVEESVIEKVTV

Target ORF information:

RefSeq Version	XM_008760014.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760014.2

ORF Insert Sequence:

ATGGGACTTG CAGCTTCTTT GCAAAGGGAG AAAGAAGTGA CCACAGTGAC TGTGGCCAAC 
GTGGGGTCCT CTGCAGATAA CGTCTTCACA ACATCAGTGG CAAATGCAGC CTCGATATCA 
GGACATGTCC TGTCTGGTAG GACAGCCCTG CAGATCGGGG ACAGCCTGAA CACTGAGAAA 
GCCACACTAA TAGTTGTACA CACAGATGGG AGCATTGTGG AAACCACCGG GCTGAAGGGC 
CCAGCAGCAC CTCTCACCCC AGCCTCTCTT TGCCCAGGTC CTCAGTCTCC TCCTACCCCA 
CTGGCACCTG GCCAGGAGAA AGGTGGTACC AAGTACAACT GGGACCCTTC GGTGTATGAC 
AGCGAGCTGC CTGTGCGCTG TCGGAACATC AGTGGCACGC TCTACAAGAG TCGGCTCGGC 
TCAGGTGGCC GGGGGCGGTG TATCAAGCAG GGAGAAAACT GGTACAGCCC AACCGAGTTT 
GAAGCCATGG CAGGAAGAGC CAGCAGCAAG GACTGGAAGA GAAGCATCCG CTATGCGGGC 
AGACCCCTGC AGTGCCTCAT CCAGGATGGT ATTTTGAACC CTCACGCTGC CTCCTGCACC 
TGTGCTGCCT GTTGCGATGA CATGACTCTA AGTGGCCCTG TCAGGCTCTT CGTCCCCTAT 
AAAAGGCGCA AGAAAGAGAA TGAGCTGCCC ACGACTCCAG TTAAGAAGGA TTCCCCCAAG 
AACATCACGC TGCTCCCTGC CACGGCGGCC ACCACCTTCA CTGTGACACC CTCAGGACAG 
ATCACTACCT CTGGAGCACT AACCTTTGAC CGAGCATCCA CTGTGGAGGC CACTGCTGTC 
ATCTCTGAGA GCCCAGCCCA AGGGGATGTC TTTGCAGGAG CCACAGTGCA AGAGGCAGGC 
GTGCAGCCCC CCTGCAGGGT TGGCCACCCT GAACCCCACT ACCCTGGCTA TCAGGACAGC 
TGCCAGATCG CCCCGTTTCC AGAAGCTGCA TTGCCAACAT CACATCCCAA AATTGTCCTG 
ACATCGCTGC CCGCCTTGGC CGTGCCGCCC TCCACCCCCA CCAAAGCTGT CTCTCCCACC 
GTGGTCAGTG GGCTGGAGAT GTCAGAGCAT CGGAGCTGGC TGTACCTGGA AGAGATGGTC 
AACTCCCTCC TCAACACAGC CCAGCAGCTG AAGACGTTGT TTGAACAAGC CAAGCAGGCG 
AGCTCTTGCA GGGAAGCTGC TGTGACCCAG GCGAGAATGC AGGTTGATGC AGAGAGGAAG 
GAGCAGTCAT GTGTCAACTG CGGCCGGGAG GCCATGAGTG AGTGCACCGG CTGCCACAAG 
GTTAACTACT GCTCTACGTT CTGCCAGCGC AAGGACTGGA AAGACCATCA GCATGTATGT 
GGCCAGTCAG CATCTGTCAC TGTCCAGGCT GATGACGTCC ATGTTGAAGA AAGTGTGATA 
GAAAAAGTTA CTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Deaf1 ( NM_031801.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113989.2 Rattus norvegicus DEAF1 transcription factor (Deaf1), mRNA.

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CloneID	ORa43777
Clone ID Related Accession (Same CDS sequence)	NM_031801.2
Accession Version	NM_031801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Rattus norvegicus(Norway rat)
Product	deformed epidermal autoregulatory factor 1 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC094507.9 and AC118351.14. On May 28, 2015 this sequence version replaced NM_031801.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF055884.1, U59659.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGACT CGGACTCGGC GGCAAAACAG CTGGGCCTGG CCGAGGCGGC AGCGGTGGCG 
GCCGCTGCCG CTGTGGCGGC GGCCGCCGCG GCCGCAGCGG AAAGCGAGGC GGAGGAGCCG 
GTGTTAAGCA GAGACGAGGA CTCGGAGGAG GACGCAGACT CCGAGGCGGA GCGCGAGACG 
CGGCGGGTCA CGGCTGTGGC GGTGATGGCG GCCGAATCTG GGCACATGGA TATGGGTACC 
GAGGCTCTGC CCAGCCCTGA TGAGGCCGCC GCCGCCGCCG CCTTTGCAGA AGTGACCACA 
GTGACTGTGG CCAACGTGGG GTCCTCTGCA GATAACGTCT TCACAACATC AGTGGCAAAT 
GCAGCCTCGA TATCAGGACA TGTCCTGTCT GGTAGGACAG CCCTGCAGAT CGGGGACAGC 
CTGAACACTG AGAAAGCCAC ACTAATAGTT GTACACACAG ATGGGAGCAT TGTGGAAACC 
ACCGGGCTGA AGGGCCCAGC AGCACCTCTC ACCCCAGGTC CTCAGTCTCC TCCTACCCCA 
CTGGCACCTG GCCAGGAGAA AGGTGGTACC AAGTACAACT GGGACCCTTC GGTGTATGAC 
AGCGAGCTGC CTGTGCGCTG TCGGAACATC AGTGGCACGC TCTACAAGAG TCGGCTCGGC 
TCAGGTGGCC GGGGGCGGTG TATCAAGCAG GGAGAAAACT GGTACAGCCC AACCGAGTTT 
GAAGCCATGG CAGGAAGAGC CAGCAGCAAG GACTGGAAGA GAAGCATCCG CTATGCGGGC 
AGACCCCTGC AGTGCCTCAT CCAGGATGGT ATTTTGAACC CTCACGCTGC CTCCTGCACC 
TGTGCTGCCT GTTGCGATGA CATGACTCTA AGTGGCCCTG TCAGGCTCTT CGTCCCCTAT 
AAAAGGCGCA AGAAAGAGAA TGAGCTGCCC ACGACTCCAG TTAAGAAGGA TTCCCCCAAG 
AACATCACGC TGCTCCCTGC CACGGCGGCC ACCACCTTCA CTGTGACACC CTCAGGACAG 
ATCACTACCT CTGGAGCACT AACCTTTGAC CGAGCATCCA CTGTGGAGGC CACTGCTGTC 
ATCTCTGAGA GCCCAGCCCA AGGGGATGTC TTTGCAGGAG CCACAGTGCA AGAGGCAGGC 
GTGCAGCCCC CCTGCAGGGT TGGCCACCCT GAACCCCACT ACCCTGGCTA TCAGGACAGC 
TGCCAGATCG CCCCGTTTCC AGAAGCTGCA TTGCCAACAT CACATCCCAA AATTGTCCTG 
ACATCGCTGC CCGCCTTGGC CGTGCCGCCC TCCACCCCCA CCAAAGCTGT CTCTCCCACC 
GTGGTCAGTG GGCTGGAGAT GTCAGAGCAT CGGAGCTGGC TGTACCTGGA AGAGATGGTC 
AACTCCCTCC TCAACACAGC CCAGCAGCTG AAGACGTTGT TTGAACAAGC CAAGCAGGCG 
AGCTCTTGCA GGGAAGCTGC TGTGACCCAG GCGAGAATGC AGGTTGATGC AGAGAGGAAG 
GAGCAGTCAT GTGTCAACTG CGGCCGGGAG GCCATGAGTG AGTGCACCGG CTGCCACAAG 
GTTAACTACT GCTCTACGTT CTGCCAGCGC AAGGACTGGA AAGACCATCA GCATGTATGT 
GGCCAGTCAG CATCTGTCAC TGTCCAGGCT GATGACGTCC ATGTTGAAGA AAGTGTGATA 
GAAAAAGTTA CTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113989.2
CDS	103..1800
Translation	MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAESEAEEPVLSRDEDSEEDADSEAERETRRVTAVAVMAAESGHMDMGTEALPSPDEAAAAAAFAEVTTVTVANVGSSADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKSRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGATVQEAGVQPPCRVGHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPSTPTKAVSPTVVSGLEMSEHRSWLYLEEMVNSLLNTAQQLKTLFEQAKQASSCREAAVTQARMQVDAERKEQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHVCGQSASVTVQADDVHVEESVIEKVTV

Target ORF information:

RefSeq Version	NM_031801.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAF1 transcription factor (Deaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031801.2

ORF Insert Sequence:

ATGGAGGACT CGGACTCGGC GGCAAAACAG CTGGGCCTGG CCGAGGCGGC AGCGGTGGCG 
GCCGCTGCCG CTGTGGCGGC GGCCGCCGCG GCCGCAGCGG AAAGCGAGGC GGAGGAGCCG 
GTGTTAAGCA GAGACGAGGA CTCGGAGGAG GACGCAGACT CCGAGGCGGA GCGCGAGACG 
CGGCGGGTCA CGGCTGTGGC GGTGATGGCG GCCGAATCTG GGCACATGGA TATGGGTACC 
GAGGCTCTGC CCAGCCCTGA TGAGGCCGCC GCCGCCGCCG CCTTTGCAGA AGTGACCACA 
GTGACTGTGG CCAACGTGGG GTCCTCTGCA GATAACGTCT TCACAACATC AGTGGCAAAT 
GCAGCCTCGA TATCAGGACA TGTCCTGTCT GGTAGGACAG CCCTGCAGAT CGGGGACAGC 
CTGAACACTG AGAAAGCCAC ACTAATAGTT GTACACACAG ATGGGAGCAT TGTGGAAACC 
ACCGGGCTGA AGGGCCCAGC AGCACCTCTC ACCCCAGGTC CTCAGTCTCC TCCTACCCCA 
CTGGCACCTG GCCAGGAGAA AGGTGGTACC AAGTACAACT GGGACCCTTC GGTGTATGAC 
AGCGAGCTGC CTGTGCGCTG TCGGAACATC AGTGGCACGC TCTACAAGAG TCGGCTCGGC 
TCAGGTGGCC GGGGGCGGTG TATCAAGCAG GGAGAAAACT GGTACAGCCC AACCGAGTTT 
GAAGCCATGG CAGGAAGAGC CAGCAGCAAG GACTGGAAGA GAAGCATCCG CTATGCGGGC 
AGACCCCTGC AGTGCCTCAT CCAGGATGGT ATTTTGAACC CTCACGCTGC CTCCTGCACC 
TGTGCTGCCT GTTGCGATGA CATGACTCTA AGTGGCCCTG TCAGGCTCTT CGTCCCCTAT 
AAAAGGCGCA AGAAAGAGAA TGAGCTGCCC ACGACTCCAG TTAAGAAGGA TTCCCCCAAG 
AACATCACGC TGCTCCCTGC CACGGCGGCC ACCACCTTCA CTGTGACACC CTCAGGACAG 
ATCACTACCT CTGGAGCACT AACCTTTGAC CGAGCATCCA CTGTGGAGGC CACTGCTGTC 
ATCTCTGAGA GCCCAGCCCA AGGGGATGTC TTTGCAGGAG CCACAGTGCA AGAGGCAGGC 
GTGCAGCCCC CCTGCAGGGT TGGCCACCCT GAACCCCACT ACCCTGGCTA TCAGGACAGC 
TGCCAGATCG CCCCGTTTCC AGAAGCTGCA TTGCCAACAT CACATCCCAA AATTGTCCTG 
ACATCGCTGC CCGCCTTGGC CGTGCCGCCC TCCACCCCCA CCAAAGCTGT CTCTCCCACC 
GTGGTCAGTG GGCTGGAGAT GTCAGAGCAT CGGAGCTGGC TGTACCTGGA AGAGATGGTC 
AACTCCCTCC TCAACACAGC CCAGCAGCTG AAGACGTTGT TTGAACAAGC CAAGCAGGCG 
AGCTCTTGCA GGGAAGCTGC TGTGACCCAG GCGAGAATGC AGGTTGATGC AGAGAGGAAG 
GAGCAGTCAT GTGTCAACTG CGGCCGGGAG GCCATGAGTG AGTGCACCGG CTGCCACAAG 
GTTAACTACT GCTCTACGTT CTGCCAGCGC AAGGACTGGA AAGACCATCA GCATGTATGT 
GGCCAGTCAG CATCTGTCAC TGTCCAGGCT GATGACGTCC ATGTTGAAGA AAGTGTGATA 
GAAAAAGTTA CTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Deaf1 ( XM_008760011.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758233.1 Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X1, mRNA.

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CloneID	ORa43771
Clone ID Related Accession (Same CDS sequence)	XM_008760011.2
Accession Version	XM_008760011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	deformed epidermal autoregulatory factor 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT CGGACTCGGC GGCAAAACAG CTGGGCCTGG CCGAGGCGGC AGCGGTGGCG 
GCCGCTGCCG CTGTGGCGGC GGCCGCCGCG GCCGCAGCGG AAAGCGAGGC GGAGGAGCCG 
GTGTTAAGCA GAGACGAGGA CTCGGAGGAG GACGCAGACT CCGAGGCGGA GCGCGAGACG 
CGGCGGGTCA CGGCTGTGGC GGTGATGGCG GCCGAATCTG GGCACATGGA TATGGGTACC 
GAGGCTCTGC CCAGCCCTGA TGAGGCCGCC GCCGCCGCCG CCTTTGCAGA AGTGACCACA 
GTGACTGTGG CCAACGTGGG GTCCTCTGCA GATAACGTCT TCACAACATC AGTGGCAAAT 
GCAGCCTCGA TATCAGGACA TGTCCTGTCT GGTAGGACAG CCCTGCAGAT CGGGGACAGC 
CTGAACACTG AGAAAGCCAC ACTAATAGTT GTACACACAG ATGGGAGCAT TGTGGAAACC 
ACCGGGCTGA AGGGCCCAGC AGCACCTCTC ACCCCAGCCT CTCTTTGCCC AGGTCCTCAG 
TCTCCTCCTA CCCCACTGGC ACCTGGCCAG GAGAAAGGTG GTACCAAGTA CAACTGGGAC 
CCTTCGGTGT ATGACAGCGA GCTGCCTGTG CGCTGTCGGA ACATCAGTGG CACGCTCTAC 
AAGAGTCGGC TCGGCTCAGG TGGCCGGGGG CGGTGTATCA AGCAGGGAGA AAACTGGTAC 
AGCCCAACCG AGTTTGAAGC CATGGCAGGA AGAGCCAGCA GCAAGGACTG GAAGAGAAGC 
ATCCGCTATG CGGGCAGACC CCTGCAGTGC CTCATCCAGG ATGGTATTTT GAACCCTCAC 
GCTGCCTCCT GCACCTGTGC TGCCTGTTGC GATGACATGA CTCTAAGTGG CCCTGTCAGG 
CTCTTCGTCC CCTATAAAAG GCGCAAGAAA GAGAATGAGC TGCCCACGAC TCCAGTTAAG 
AAGGATTCCC CCAAGAACAT CACGCTGCTC CCTGCCACGG CGGCCACCAC CTTCACTGTG 
ACACCCTCAG GACAGATCAC TACCTCTGGA GCACTAACCT TTGACCGAGC ATCCACTGTG 
GAGGCCACTG CTGTCATCTC TGAGAGCCCA GCCCAAGGGG ATGTCTTTGC AGGAGCCACA 
GTGCAAGAGG CAGGCGTGCA GCCCCCCTGC AGGGTTGGCC ACCCTGAACC CCACTACCCT 
GGCTATCAGG ACAGCTGCCA GATCGCCCCG TTTCCAGAAG CTGCATTGCC AACATCACAT 
CCCAAAATTG TCCTGACATC GCTGCCCGCC TTGGCCGTGC CGCCCTCCAC CCCCACCAAA 
GCTGTCTCTC CCACCGTGGT CAGTGGGCTG GAGATGTCAG AGCATCGGAG CTGGCTGTAC 
CTGGAAGAGA TGGTCAACTC CCTCCTCAAC ACAGCCCAGC AGCTGAAGAC GTTGTTTGAA 
CAAGCCAAGC AGGCGAGCTC TTGCAGGGAA GCTGCTGTGA CCCAGGCGAG AATGCAGGTT 
GATGCAGAGA GGAAGGAGCA GTCATGTGTC AACTGCGGCC GGGAGGCCAT GAGTGAGTGC 
ACCGGCTGCC ACAAGGTTAA CTACTGCTCT ACGTTCTGCC AGCGCAAGGA CTGGAAAGAC 
CATCAGCATG TATGTGGCCA GTCAGCATCT GTCACTGTCC AGGCTGATGA CGTCCATGTT 
GAAGAAAGTG TGATAGAAAA AGTTACTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758233.1
CDS	21..1733
Translation	MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAESEAEEPVLSRDEDSEEDADSEAERETRRVTAVAVMAAESGHMDMGTEALPSPDEAAAAAAFAEVTTVTVANVGSSADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPASLCPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKSRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGATVQEAGVQPPCRVGHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPSTPTKAVSPTVVSGLEMSEHRSWLYLEEMVNSLLNTAQQLKTLFEQAKQASSCREAAVTQARMQVDAERKEQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHVCGQSASVTVQADDVHVEESVIEKVTV

Target ORF information:

RefSeq Version	XM_008760011.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760011.2

ORF Insert Sequence:

ATGGAGGACT CGGACTCGGC GGCAAAACAG CTGGGCCTGG CCGAGGCGGC AGCGGTGGCG 
GCCGCTGCCG CTGTGGCGGC GGCCGCCGCG GCCGCAGCGG AAAGCGAGGC GGAGGAGCCG 
GTGTTAAGCA GAGACGAGGA CTCGGAGGAG GACGCAGACT CCGAGGCGGA GCGCGAGACG 
CGGCGGGTCA CGGCTGTGGC GGTGATGGCG GCCGAATCTG GGCACATGGA TATGGGTACC 
GAGGCTCTGC CCAGCCCTGA TGAGGCCGCC GCCGCCGCCG CCTTTGCAGA AGTGACCACA 
GTGACTGTGG CCAACGTGGG GTCCTCTGCA GATAACGTCT TCACAACATC AGTGGCAAAT 
GCAGCCTCGA TATCAGGACA TGTCCTGTCT GGTAGGACAG CCCTGCAGAT CGGGGACAGC 
CTGAACACTG AGAAAGCCAC ACTAATAGTT GTACACACAG ATGGGAGCAT TGTGGAAACC 
ACCGGGCTGA AGGGCCCAGC AGCACCTCTC ACCCCAGCCT CTCTTTGCCC AGGTCCTCAG 
TCTCCTCCTA CCCCACTGGC ACCTGGCCAG GAGAAAGGTG GTACCAAGTA CAACTGGGAC 
CCTTCGGTGT ATGACAGCGA GCTGCCTGTG CGCTGTCGGA ACATCAGTGG CACGCTCTAC 
AAGAGTCGGC TCGGCTCAGG TGGCCGGGGG CGGTGTATCA AGCAGGGAGA AAACTGGTAC 
AGCCCAACCG AGTTTGAAGC CATGGCAGGA AGAGCCAGCA GCAAGGACTG GAAGAGAAGC 
ATCCGCTATG CGGGCAGACC CCTGCAGTGC CTCATCCAGG ATGGTATTTT GAACCCTCAC 
GCTGCCTCCT GCACCTGTGC TGCCTGTTGC GATGACATGA CTCTAAGTGG CCCTGTCAGG 
CTCTTCGTCC CCTATAAAAG GCGCAAGAAA GAGAATGAGC TGCCCACGAC TCCAGTTAAG 
AAGGATTCCC CCAAGAACAT CACGCTGCTC CCTGCCACGG CGGCCACCAC CTTCACTGTG 
ACACCCTCAG GACAGATCAC TACCTCTGGA GCACTAACCT TTGACCGAGC ATCCACTGTG 
GAGGCCACTG CTGTCATCTC TGAGAGCCCA GCCCAAGGGG ATGTCTTTGC AGGAGCCACA 
GTGCAAGAGG CAGGCGTGCA GCCCCCCTGC AGGGTTGGCC ACCCTGAACC CCACTACCCT 
GGCTATCAGG ACAGCTGCCA GATCGCCCCG TTTCCAGAAG CTGCATTGCC AACATCACAT 
CCCAAAATTG TCCTGACATC GCTGCCCGCC TTGGCCGTGC CGCCCTCCAC CCCCACCAAA 
GCTGTCTCTC CCACCGTGGT CAGTGGGCTG GAGATGTCAG AGCATCGGAG CTGGCTGTAC 
CTGGAAGAGA TGGTCAACTC CCTCCTCAAC ACAGCCCAGC AGCTGAAGAC GTTGTTTGAA 
CAAGCCAAGC AGGCGAGCTC TTGCAGGGAA GCTGCTGTGA CCCAGGCGAG AATGCAGGTT 
GATGCAGAGA GGAAGGAGCA GTCATGTGTC AACTGCGGCC GGGAGGCCAT GAGTGAGTGC 
ACCGGCTGCC ACAAGGTTAA CTACTGCTCT ACGTTCTGCC AGCGCAAGGA CTGGAAAGAC 
CATCAGCATG TATGTGGCCA GTCAGCATCT GTCACTGTCC AGGCTGATGA CGTCCATGTT 
GAAGAAAGTG TGATAGAAAA AGTTACTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Deaf1 ( XM_008760012.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758234.1 Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X2, mRNA.

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CloneID	ORa43772
Clone ID Related Accession (Same CDS sequence)	XM_008760012.2
Accession Version	XM_008760012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	deformed epidermal autoregulatory factor 1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT CGGACTCGGC GGCAAAACAG CTGGGCCTGG CCGAGGCGGC AGCGGTGGCG 
GCCGCTGCCG CTGTGGCGGC GGCCGCCGCG GCCGCAGCGG AAAGCGAGGC GGAGGAGCCG 
GTGTTAAGCA GAGACGAGGA CTCGGAGGAG GACGCAGACT CCGAGGCGGA GCGCGAGACG 
CGGCGGGTCA CGGCTGTGGC GGTGATGGCG GCCGAATCTG GGCACATGGA TATGGGTACC 
GAGGCTCTGC CCAGCCCTGA TGAGGCCGCC GCCGCCGCCG CCTTTGCAGA AGTGACCACA 
GTGACTGTGG CCAACGTGGG GTCCTCTGCA GATAACGTCT TCACAACATC AGTGGCAAAT 
GCAGCCTCGA TATCAGGACA TGTCCTGTCT GGTAGGACAG CCCTGCAGAT CGGGGACAGC 
CTGAACACTG AGAAAGCCAC ACTAATAGTT GTACACACAG ATGGGAGCAT TGTGGAAACC 
ACCGGGCTGA AGGGCCCAGC AGCACCTCTC ACCCCAGCCT CTCTTTGCCC AGGTCCTCAG 
TCTCCTCCTA CCCCACTGGC ACCTGGCCAG GAGAAAGGTG GTACCAAGTA CAACTGGGAC 
CCTTCGGTGT ATGACAGCGA GCTGCCTGTG CGCTGTCGGA ACATCAGTGG CACGCTCTAC 
AAGAGTCGGC TCGGCTCAGG TGGCCGGGGG CGGTGTATCA AGCAGGGAGA AAACTGGTAC 
AGCCCAACCG AGTTTGAAGC CATGGCAGGA AGAGCCAGCA GCAAGGACTG GAAGAGAAGC 
ATCCGCTATG CGGGCAGACC CCTGCAGTGC CTCATCCAGG ATGGTATTTT GAACCCTCAC 
GCTGCCTCCT GCACCTGTGC TGCCTGTTGC GATGACATGA CTCTAAGTGG CCCTGTCAGG 
CTCTTCGTCC CCTATAAAAG GCGCAAGAAA GAGAATGAGC TGCCCACGAC TCCAGTTAAG 
AAGGATTCCC CCAAGAACAT CACGCTGCTC CCTGCCACGG CGGCCACCAC CTTCACTGTG 
ACACCCTCAG GACAGATCAC TACCTCTGGA GCACTAACCT TTGACCGAGC ATCCACTGTG 
GAGGCCACTG CTGTCATCTC TGAGAGCCCA GCCCAAGGGG ATGTCTTTGC AGGAGCCACA 
GTGCAAGAGG CAGGCGTGCA GCCCCCCTGC AGGGTTGGCC ACCCTGAACC CCACTACCCT 
GGCTATCAGG ACAGCTGCCA GATCGCCCCG TTTCCAGAAG CTGCATTGCC AACATCACAT 
CCCAAAATTG TCCTGACATC GCTGCCCGCC TTGGCCGTGC CGCCCTCCAC CCCCACCAAA 
GCTGTCTCTC CCACCGTGGT CAGTGGGCTG GAGATGTCAG AGCATCGGAG CTGGCTGTAC 
CTGGAAGAGA TGGTCAACTC CCTCCTCAAC ACAGCCCAGC AGCTGAAGAC GTTGTTTGAA 
CAAGCCAAGC AGGCGAGCTC TTGCAGGGAA GCTGCTGTGA CCCAGGCGAG AATGCAGGTT 
GATGCAGAGA GGAAGGAGTC ATGTGTCAAC TGCGGCCGGG AGGCCATGAG TGAGTGCACC 
GGCTGCCACA AGGTTAACTA CTGCTCTACG TTCTGCCAGC GCAAGGACTG GAAAGACCAT 
CAGCATGTAT GTGGCCAGTC AGCATCTGTC ACTGTCCAGG CTGATGACGT CCATGTTGAA 
GAAAGTGTGA TAGAAAAAGT TACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758234.1
CDS	21..1730
Translation	MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAESEAEEPVLSRDEDSEEDADSEAERETRRVTAVAVMAAESGHMDMGTEALPSPDEAAAAAAFAEVTTVTVANVGSSADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPASLCPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKSRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGATVQEAGVQPPCRVGHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPSTPTKAVSPTVVSGLEMSEHRSWLYLEEMVNSLLNTAQQLKTLFEQAKQASSCREAAVTQARMQVDAERKESCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHVCGQSASVTVQADDVHVEESVIEKVTV

Target ORF information:

RefSeq Version	XM_008760012.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760012.2

ORF Insert Sequence:

ATGGAGGACT CGGACTCGGC GGCAAAACAG CTGGGCCTGG CCGAGGCGGC AGCGGTGGCG 
GCCGCTGCCG CTGTGGCGGC GGCCGCCGCG GCCGCAGCGG AAAGCGAGGC GGAGGAGCCG 
GTGTTAAGCA GAGACGAGGA CTCGGAGGAG GACGCAGACT CCGAGGCGGA GCGCGAGACG 
CGGCGGGTCA CGGCTGTGGC GGTGATGGCG GCCGAATCTG GGCACATGGA TATGGGTACC 
GAGGCTCTGC CCAGCCCTGA TGAGGCCGCC GCCGCCGCCG CCTTTGCAGA AGTGACCACA 
GTGACTGTGG CCAACGTGGG GTCCTCTGCA GATAACGTCT TCACAACATC AGTGGCAAAT 
GCAGCCTCGA TATCAGGACA TGTCCTGTCT GGTAGGACAG CCCTGCAGAT CGGGGACAGC 
CTGAACACTG AGAAAGCCAC ACTAATAGTT GTACACACAG ATGGGAGCAT TGTGGAAACC 
ACCGGGCTGA AGGGCCCAGC AGCACCTCTC ACCCCAGCCT CTCTTTGCCC AGGTCCTCAG 
TCTCCTCCTA CCCCACTGGC ACCTGGCCAG GAGAAAGGTG GTACCAAGTA CAACTGGGAC 
CCTTCGGTGT ATGACAGCGA GCTGCCTGTG CGCTGTCGGA ACATCAGTGG CACGCTCTAC 
AAGAGTCGGC TCGGCTCAGG TGGCCGGGGG CGGTGTATCA AGCAGGGAGA AAACTGGTAC 
AGCCCAACCG AGTTTGAAGC CATGGCAGGA AGAGCCAGCA GCAAGGACTG GAAGAGAAGC 
ATCCGCTATG CGGGCAGACC CCTGCAGTGC CTCATCCAGG ATGGTATTTT GAACCCTCAC 
GCTGCCTCCT GCACCTGTGC TGCCTGTTGC GATGACATGA CTCTAAGTGG CCCTGTCAGG 
CTCTTCGTCC CCTATAAAAG GCGCAAGAAA GAGAATGAGC TGCCCACGAC TCCAGTTAAG 
AAGGATTCCC CCAAGAACAT CACGCTGCTC CCTGCCACGG CGGCCACCAC CTTCACTGTG 
ACACCCTCAG GACAGATCAC TACCTCTGGA GCACTAACCT TTGACCGAGC ATCCACTGTG 
GAGGCCACTG CTGTCATCTC TGAGAGCCCA GCCCAAGGGG ATGTCTTTGC AGGAGCCACA 
GTGCAAGAGG CAGGCGTGCA GCCCCCCTGC AGGGTTGGCC ACCCTGAACC CCACTACCCT 
GGCTATCAGG ACAGCTGCCA GATCGCCCCG TTTCCAGAAG CTGCATTGCC AACATCACAT 
CCCAAAATTG TCCTGACATC GCTGCCCGCC TTGGCCGTGC CGCCCTCCAC CCCCACCAAA 
GCTGTCTCTC CCACCGTGGT CAGTGGGCTG GAGATGTCAG AGCATCGGAG CTGGCTGTAC 
CTGGAAGAGA TGGTCAACTC CCTCCTCAAC ACAGCCCAGC AGCTGAAGAC GTTGTTTGAA 
CAAGCCAAGC AGGCGAGCTC TTGCAGGGAA GCTGCTGTGA CCCAGGCGAG AATGCAGGTT 
GATGCAGAGA GGAAGGAGTC ATGTGTCAAC TGCGGCCGGG AGGCCATGAG TGAGTGCACC 
GGCTGCCACA AGGTTAACTA CTGCTCTACG TTCTGCCAGC GCAAGGACTG GAAAGACCAT 
CAGCATGTAT GTGGCCAGTC AGCATCTGTC ACTGTCCAGG CTGATGACGT CCATGTTGAA 
GAAAGTGTGA TAGAAAAAGT TACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Deaf1 ( XM_008760013.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758235.1 Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X3, mRNA.

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CloneID	ORa43773
Clone ID Related Accession (Same CDS sequence)	XM_008760013.2
Accession Version	XM_008760013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	deformed epidermal autoregulatory factor 1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT CGGACTCGGC GGCAAAACAG CTGGGCCTGG CCGAGGCGGC AGCGGTGGCG 
GCCGCTGCCG CTGTGGCGGC GGCCGCCGCG GCCGCAGCGG AAAGCGAGGC GGAGGAGCCG 
GTGTTAAGCA GAGACGAGGA CTCGGAGGAG GACGCAGACT CCGAGGCGGA GCGCGAGACG 
CGGCGGGTCA CGGCTGTGGC GGTGATGGCG GCCGAATCTG GGCACATGGA TATGGGTACC 
GAGGCTCTGC CCAGCCCTGA TGAGGCCGCC GCCGCCGCCG CCTTTGCAGA AGTGACCACA 
GTGACTGTGG CCAACGTGGG GTCCTCTGCA GATAACGTCT TCACAACATC AGTGGCAAAT 
GCAGCCTCGA TATCAGGACA TGTCCTGTCT GGTAGGACAG CCCTGCAGAT CGGGGACAGC 
CTGAACACTG AGAAAGCCAC ACTAATAGTT GTACACACAG ATGGGAGCAT TGTGGAAACC 
ACCGGGCTGA AGGGCCCAGC AGCACCTCTC ACCCCAGCCT CTCTTTGCCC AGGTCCTCAG 
TCTCCTCCTA CCCCACTGGC ACCTGGCCAG GAGAAAGGTG GTACCAAGTA CAACTGGGAC 
CCTTCGGTGT ATGACAGCGA GCTGCCTGTG CGCTGTCGGA ACATCAGTGG CACGCTCTAC 
AAGAGTCGGC TCGGCTCAGG TGGCCGGGGG CGGTGTATCA AGCAGGGAGA AAACTGGTAC 
AGCCCAACCG AGTTTGAAGC CATGGCAGGA AGAGCCAGCA GCAAGGACTG GAAGAGAAGC 
ATCCGCTATG CGGGCAGACC CCTGCAGTGC CTCATCCAGG ATGGTATTTT GAACCCTCAC 
GCTGCCTCCT GCACCTGTGC TGCCTGTTGC GATGACATGA CTCTAAGTGG CCCTGTCAGG 
CTCTTCGTCC CCTATAAAAG GCGCAAGAAA GAGAATGAGC TGCCCACGAC TCCAGTTAAG 
AAGGATTCCC CCAAGAACAT CACGCTGCTC CCTGCCACGG CGGCCACCAC CTTCACTGTG 
ACACCCTCAG GACAGATCAC TACCTCTGGA GCACTAACCT TTGACCGAGC ATCCACTGTG 
GAGGCCACTG CTGTCATCTC TGAGAGCCCA GCCCAAGGGG ATGTCTTTGC AGGAGCCACA 
GTGCAAGAGG CAGGCGTGCA GCCCCCCTGC AGGGTTGGCC ACCCTGAACC CCACTACCCT 
GGCTATCAGG ACAGCTGCCA GATCGCCCCG TTTCCAGAAG CTGCATTGCC AACATCACAT 
CCCAAAATTG TCCTGACATC GCTGCCCGCC TTGGCCGTGC CGCCCTCCAC CCCCACCAAA 
GCTGTCTCTC CCACCGTGGT CAGTGGGCTG GAGATGTCAG AGCATCGGAG CTGGCTGTAC 
CTGGAAGAGA TGGTCAACTC CCTCCTCAAC ACAGCCCAGC AGCTGAAGAC GTTGTTTGAA 
CAAGCCAAGC AGGCGAGCTC TTGCAGGGAA GCTGCTGTGA CCCAGGCGAG AATGCAGGTT 
GATGCAGAGA GGAAGGAGGA CTGGAAAGAC CATCAGCATG TATGTGGCCA GTCAGCATCT 
GTCACTGTCC AGGCTGATGA CGTCCATGTT GAAGAAAGTG TGATAGAAAA AGTTACTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758235.1
CDS	21..1643
Translation	MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAESEAEEPVLSRDEDSEEDADSEAERETRRVTAVAVMAAESGHMDMGTEALPSPDEAAAAAAFAEVTTVTVANVGSSADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPASLCPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKSRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGATVQEAGVQPPCRVGHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPSTPTKAVSPTVVSGLEMSEHRSWLYLEEMVNSLLNTAQQLKTLFEQAKQASSCREAAVTQARMQVDAERKEDWKDHQHVCGQSASVTVQADDVHVEESVIEKVTV

Target ORF information:

RefSeq Version	XM_008760013.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAF1 transcription factor (Deaf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760013.2

ORF Insert Sequence:

ATGGAGGACT CGGACTCGGC GGCAAAACAG CTGGGCCTGG CCGAGGCGGC AGCGGTGGCG 
GCCGCTGCCG CTGTGGCGGC GGCCGCCGCG GCCGCAGCGG AAAGCGAGGC GGAGGAGCCG 
GTGTTAAGCA GAGACGAGGA CTCGGAGGAG GACGCAGACT CCGAGGCGGA GCGCGAGACG 
CGGCGGGTCA CGGCTGTGGC GGTGATGGCG GCCGAATCTG GGCACATGGA TATGGGTACC 
GAGGCTCTGC CCAGCCCTGA TGAGGCCGCC GCCGCCGCCG CCTTTGCAGA AGTGACCACA 
GTGACTGTGG CCAACGTGGG GTCCTCTGCA GATAACGTCT TCACAACATC AGTGGCAAAT 
GCAGCCTCGA TATCAGGACA TGTCCTGTCT GGTAGGACAG CCCTGCAGAT CGGGGACAGC 
CTGAACACTG AGAAAGCCAC ACTAATAGTT GTACACACAG ATGGGAGCAT TGTGGAAACC 
ACCGGGCTGA AGGGCCCAGC AGCACCTCTC ACCCCAGCCT CTCTTTGCCC AGGTCCTCAG 
TCTCCTCCTA CCCCACTGGC ACCTGGCCAG GAGAAAGGTG GTACCAAGTA CAACTGGGAC 
CCTTCGGTGT ATGACAGCGA GCTGCCTGTG CGCTGTCGGA ACATCAGTGG CACGCTCTAC 
AAGAGTCGGC TCGGCTCAGG TGGCCGGGGG CGGTGTATCA AGCAGGGAGA AAACTGGTAC 
AGCCCAACCG AGTTTGAAGC CATGGCAGGA AGAGCCAGCA GCAAGGACTG GAAGAGAAGC 
ATCCGCTATG CGGGCAGACC CCTGCAGTGC CTCATCCAGG ATGGTATTTT GAACCCTCAC 
GCTGCCTCCT GCACCTGTGC TGCCTGTTGC GATGACATGA CTCTAAGTGG CCCTGTCAGG 
CTCTTCGTCC CCTATAAAAG GCGCAAGAAA GAGAATGAGC TGCCCACGAC TCCAGTTAAG 
AAGGATTCCC CCAAGAACAT CACGCTGCTC CCTGCCACGG CGGCCACCAC CTTCACTGTG 
ACACCCTCAG GACAGATCAC TACCTCTGGA GCACTAACCT TTGACCGAGC ATCCACTGTG 
GAGGCCACTG CTGTCATCTC TGAGAGCCCA GCCCAAGGGG ATGTCTTTGC AGGAGCCACA 
GTGCAAGAGG CAGGCGTGCA GCCCCCCTGC AGGGTTGGCC ACCCTGAACC CCACTACCCT 
GGCTATCAGG ACAGCTGCCA GATCGCCCCG TTTCCAGAAG CTGCATTGCC AACATCACAT 
CCCAAAATTG TCCTGACATC GCTGCCCGCC TTGGCCGTGC CGCCCTCCAC CCCCACCAAA 
GCTGTCTCTC CCACCGTGGT CAGTGGGCTG GAGATGTCAG AGCATCGGAG CTGGCTGTAC 
CTGGAAGAGA TGGTCAACTC CCTCCTCAAC ACAGCCCAGC AGCTGAAGAC GTTGTTTGAA 
CAAGCCAAGC AGGCGAGCTC TTGCAGGGAA GCTGCTGTGA CCCAGGCGAG AATGCAGGTT 
GATGCAGAGA GGAAGGAGGA CTGGAAAGAC CATCAGCATG TATGTGGCCA GTCAGCATCT 
GTCACTGTCC AGGCTGATGA CGTCCATGTT GAAGAAAGTG TGATAGAAAA AGTTACTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stress-induced alterations in 5-HT1A receptor transcriptional modulators NUDR and Freud-1.
The international journal of neuropsychopharmacology17(11)1763-75(2014 Nov)
Szewczyk B,Kotarska K,Daigle M,Misztak P,Sowa-Kucma M,Rafalo A,Curzytek K,Kubera M,Basta-Kaim A,Nowak G,Albert PR

Mutations affecting the SAND domain of DEAF1 cause intellectual disability with severe speech impairment and behavioral problems.
American journal of human genetics94(5)649-61(2014 May)
Vulto-van Silfhout AT,Rajamanickam S,Jensik PJ,Vergult S,de Rocker N,Newhall KJ,Raghavan R,Reardon SN,Jarrett K,McIntyre T,Bulinski J,Ownby SL,Huggenvik JI,McKnight GS,Rose GM,Cai X,Willaert A,Zweier C,Endele S,de Ligt J,van Bon BW,Lugtenberg D,de Vries PF,Veltman JA,van Bokhoven H,Brunner HG,Rauch A,de Brouwer AP,Carvill GL,Hoischen A,Mefford HC,Eichler EE,Vissers LE,Menten B,Collard MW,de Vries BB

Chronic social defeat downregulates the 5-HT1A receptor but not Freud-1 or NUDR in the rat prefrontal cortex.
Neuroscience letters469(3)380-4(2010 Jan)
Kieran N,Ou XM,Iyo AH

Differential regulation of the serotonin 1 A transcriptional modulators five prime repressor element under dual repression-1 and nuclear-deformed epidermal autoregulatory factor by chronic stress.
Neuroscience163(4)1119-27(2009 Nov)
Iyo AH,Kieran N,Chandran A,Albert PR,Wicks I,Bissette G,Austin MC

Deaf1 isoforms control the expression of genes encoding peripheral tissue antigens in the pancreatic lymph nodes during type 1 diabetes.
Nature immunology10(9)1026-33(2009 Sep)
Yip L,Su L,Sheng D,Chang P,Atkinson M,Czesak M,Albert PR,Collier AR,Turley SJ,Fathman CG,Creusot RJ

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Deaf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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