Trim3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim3
Entrez Gene ID	83616
Full Name	tripartite motif-containing 3
Synonyms	Berp,Rnf22
General protein information	Preferred Names tripartite motif-containing 3 Names tripartite motif-containing protein 3 brain-expressed RING finger protein ring finger protein 22 tripartite motif protein 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q32
Summary	interacts with myosin V; may be involved in myosin V-mediated cargo transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031786.1	NP_113974.1	tripartite motif-containing protein 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRIM3	NP_001234935.1
Pan troglodytes (chimpanzee)	TRIM3	XP_001164502.2
	TRIM3	XP_001109605.1
Bos taurus (cattle)	TRIM3	NP_001094627.1
Danio rerio (zebrafish)	trim3b	NP_001073637.1
Danio rerio (zebrafish)	trim3a	XP_686295.4
Canis lupus familiaris (dog)	TRIM3	XP_534038.2
Mus musculus (house mouse)	Trim3	NP_001272799.1
Gallus gallus (chicken)	TRIM3	XP_004939021.1
Rattus norvegicus (Norway rat)	Trim3	NP_113974.1

Gene Ontology
Bibliography

Related articles in PubMed

The ability of TRIM3 to induce growth arrest depends on RING-dependent E3 ligase activity.
Raheja R, Liu Y, Hukkelhoven E, Yeh N, Koff A
The Biochemical journal458(3)537-45(2014 Mar)

Degradation of postsynaptic scaffold GKAP and regulation of dendritic spine morphology by the TRIM3 ubiquitin ligase in rat hippocampal neurons.
Hung AY, Sung CC, Brito IL, Sheng M
PloS one5(3)e9842(2010 Mar)

Involvement of myosin Vb in glutamate receptor trafficking.
Lis? MF, Wong TP, Trinh A, Hines RM, Liu L, Kang R, Hines DJ, Lu J, Goldenring JR, Wang YT, El-Husseini A
The Journal of biological chemistry281(6)3669-78(2006 Feb)

The tripartite motif family identifies cell compartments.
Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A
The EMBO journal20(9)2140-51(2001 May)

BERP, a novel ring finger protein, binds to alpha-actinin-4.
El-Husseini AE, Kwasnicka D, Yamada T, Hirohashi S, Vincent SR
Biochemical and biophysical research communications267(3)906-11(2000 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

postsynaptic scaffold GKAP is degraded and dendritic spine morphology is regulated by the TRIM3 ubiquitin ligase in rat hippocampal neurons
Title: Degradation of postsynaptic scaffold GKAP and regulation of dendritic spine morphology by the TRIM3 ubiquitin ligase in rat hippocampal neurons.

The following Trim3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08738	NM_031786.1 Latest version!	Rattus norvegicus tripartite motif-containing 3 (Trim3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim3 ( NM_031786.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113974.1 Rattus norvegicus tripartite motif-containing 3 (Trim3), mRNA.

Price & Availability↑

CloneID	ORa08738
Clone ID Related Accession (Same CDS sequence)	NM_031786.1
Accession Version	NM_031786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	tripartite motif-containing protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF036255.1. ##Evidence-Data-START## Transcript exon combination :: AF036255.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAAAGA GGGAGGACAG CCCTGGCCCG GAGGTGCAGC CAATGGACAA GCAGTTTCTG 
GTATGCAGTA TCTGCCTGGA TCGGTACCGG TGCCCCAAAG TTCTGCCTTG TCTACACACC 
TTCTGCGAAA GATGTCTCCA GAACTACATC CCTCCTCAGA GCCTGACACT CTCGTGTCCA 
GTATGCCGGC AGACATCCAT CCTCCCAGAA CAGGGTGTCT CAGCCCTACA AAACAACTTC 
TTCATCAGCA GCCTCATGGA GGCCATGCAG CAGGCACCTG ATGGGGCCCA CGACCCTGAA 
GACCCCCACC CCCTCAGCGC AGTGGCTGGC CGCCCTCTCT CCTGCCCCAA CCATGAAGGC 
AAGACGATGG AGTTTTACTG TGAGGCCTGT GAGACTGCCA TGTGTGGTGA GTGCCGCGCA 
GGGGAGCACC GTGAACACGG CACAGTGCTG TTGCGGGACG TGGTGGAGCA GCACAAGGCA 
GCTCTACAGC GCCAGCTTGA AGCTGTGCGT GGCCGATTGC CACAGCTGTC TGCAGCTATC 
GCCTTGGTTG GGGGCATCAG CCAGCAGCTG CAAGAGCGCA AGGCAGAGGC CCTGGCCCAG 
ATAAGTGCAG CCTTTGAGGA CCTGGAGCAA GCTCTGCAGC AGCGCAAGCA GGCTCTGGTC 
AGCGACTTGG AGAGCATTTG TGGGGCCAAG CAGAAGGTGT TGCAGACACA GTTAGACACA 
CTGCGCCAGG GTCAGGAACA CATTGGCAGT AGCTGCAGCT TTGCAGAGCA GGCACTGAGA 
CTGGGCTCTG CCCCTGAGGT TTTGCTAGTA AGAAAGCACA TGCGAGAGAG GCTGGCTGCT 
CTGGCAGCTC AGGCCTTCCC AGAAAGGCCC CATGAGAATG CACAGCTGGA ACTGGTCCTT 
GAAGTAGATG GGTTGCGGAG ATCGGTGCTC AATCTGGGTG CACTGCTCAC CACCAGTGCT 
GCTGCACACG AGACAGTGGC CACCGGCGAG GGCCTGCGCC AGGCACTGGT TGGCCAGCCT 
GCCTCACTCA CTGTCACCAC CAAAGACAAA GATGGGCGGC TGGTGCGCAC GGGCAGCGCA 
GAGCTGTGTG CAGAGATCAC TGGCCCCGAT GGCATGCGCC TTGCGGTACC GGTGGTGGAC 
CACAAAAATG GCACATATGA GCTGGTGTAC ACAGCACGTA CAGAAGGCGA CCTGCTCCTC 
TCAGTGCTGC TCTATGGACA GCCGGTGCGT GGCAGCCCTT TCCGTGTGCG TGCCCTGAGA 
CCCGGAGACT TGCCACCTTC CCCAGATGAT GTGAAGCGCC GGGTCAAGTC TCCCGGTGGT 
CCTGGCAGTC ATGTGCGCCA GAAGGCAGTG CGTAGGCCGA GCTCCATGTA CAGCACCGGT 
GGCAAACGGA AAGACAATCC AATTGTGGAT GAACTCGTCT TTCGTGTTGG CAGTCGTGGA 
AGGGAGAAAG GTGAATTCAC CAATTTACAC CCGCTGTCCG CTGCTAGCAG TGGCCGAATA 
GTGGTAGCAG ACAGCAACAA CCAATGTATT CAGGTTTTCT CCAATGAGGG CCAGTTCAAG 
TTCCGATTTG GAGTCCGTGG GCGCTCGCCT GGGCAACTAC AGCGTCCTAC GGGTGTGGCA 
GTGGACACCA ATGGAGACAT TATTGTGGCA GACTATGACA ACCGTTGGGT CAGCATCTTC 
TCTCCTGAGG GCAAGTTCAA GACCAAAATT GGAGCTGGCC GCCTCATGGG GCCCAAGGGA 
GTGGCTGTGG ACCGGAATGG GCATATCATT GTGGTGGATA ACAAGTCTTG CTGTGTCTTC 
ACCTTCCAGC CCAATGGCAA GCTTGTTGGG CGTTTTGGAG GCCGTGGGGC CACTGACCGC 
CACTTCGCAG GGCCCCACTT TGTGGCTGTG AACAACAAGA ATGAGATTGT AGTAACGGAT 
TTCCACAACC ATTCAGTGAA GGTGTACAGT GCTGATGGAG AGTTCCTCTT CAAATTTGGC 
TCACATGGCG AGGGCAATGG ACAGTTCAAT GCCCCCACGG GAGTAGCTGT GGATTCCAAT 
GGAAACATCA TCGTGGCTGA CTGGGGCAAT AGTCGCATAC AGGTATTCGA CAGCTCTGGT 
TCCTTCCTGT CCTATATCAA CACGTCTGCA GAGCCACTGT ATGGCCCACA GGGCTTGGCA 
TTGACCTCGG ATGGCCACGT AGTAGTGGCT GATGCTGGCA ACCACTGCTT TAAGGCCTAT 
CGATACCTCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113974.1
CDS	221..2455
Translation	MAKREDSPGPEVQPMDKQFLVCSICLDRYRCPKVLPCLHTFCERCLQNYIPPQSLTLSCPVCRQTSILPEQGVSALQNNFFISSLMEAMQQAPDGAHDPEDPHPLSAVAGRPLSCPNHEGKTMEFYCEACETAMCGECRAGEHREHGTVLLRDVVEQHKAALQRQLEAVRGRLPQLSAAIALVGGISQQLQERKAEALAQISAAFEDLEQALQQRKQALVSDLESICGAKQKVLQTQLDTLRQGQEHIGSSCSFAEQALRLGSAPEVLLVRKHMRERLAALAAQAFPERPHENAQLELVLEVDGLRRSVLNLGALLTTSAAAHETVATGEGLRQALVGQPASLTVTTKDKDGRLVRTGSAELCAEITGPDGMRLAVPVVDHKNGTYELVYTARTEGDLLLSVLLYGQPVRGSPFRVRALRPGDLPPSPDDVKRRVKSPGGPGSHVRQKAVRRPSSMYSTGGKRKDNPIVDELVFRVGSRGREKGEFTNLHPLSAASSGRIVVADSNNQCIQVFSNEGQFKFRFGVRGRSPGQLQRPTGVAVDTNGDIIVADYDNRWVSIFSPEGKFKTKIGAGRLMGPKGVAVDRNGHIIVVDNKSCCVFTFQPNGKLVGRFGGRGATDRHFAGPHFVAVNNKNEIVVTDFHNHSVKVYSADGEFLFKFGSHGEGNGQFNAPTGVAVDSNGNIIVADWGNSRIQVFDSSGSFLSYINTSAEPLYGPQGLALTSDGHVVVADAGNHCFKAYRYLQ

Target ORF information:

RefSeq Version	NM_031786.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripartite motif-containing 3 (Trim3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031786.1

ORF Insert Sequence:

ATGGCAAAGA GGGAGGACAG CCCTGGCCCG GAGGTGCAGC CAATGGACAA GCAGTTTCTG 
GTATGCAGTA TCTGCCTGGA TCGGTACCGG TGCCCCAAAG TTCTGCCTTG TCTACACACC 
TTCTGCGAAA GATGTCTCCA GAACTACATC CCTCCTCAGA GCCTGACACT CTCGTGTCCA 
GTATGCCGGC AGACATCCAT CCTCCCAGAA CAGGGTGTCT CAGCCCTACA AAACAACTTC 
TTCATCAGCA GCCTCATGGA GGCCATGCAG CAGGCACCTG ATGGGGCCCA CGACCCTGAA 
GACCCCCACC CCCTCAGCGC AGTGGCTGGC CGCCCTCTCT CCTGCCCCAA CCATGAAGGC 
AAGACGATGG AGTTTTACTG TGAGGCCTGT GAGACTGCCA TGTGTGGTGA GTGCCGCGCA 
GGGGAGCACC GTGAACACGG CACAGTGCTG TTGCGGGACG TGGTGGAGCA GCACAAGGCA 
GCTCTACAGC GCCAGCTTGA AGCTGTGCGT GGCCGATTGC CACAGCTGTC TGCAGCTATC 
GCCTTGGTTG GGGGCATCAG CCAGCAGCTG CAAGAGCGCA AGGCAGAGGC CCTGGCCCAG 
ATAAGTGCAG CCTTTGAGGA CCTGGAGCAA GCTCTGCAGC AGCGCAAGCA GGCTCTGGTC 
AGCGACTTGG AGAGCATTTG TGGGGCCAAG CAGAAGGTGT TGCAGACACA GTTAGACACA 
CTGCGCCAGG GTCAGGAACA CATTGGCAGT AGCTGCAGCT TTGCAGAGCA GGCACTGAGA 
CTGGGCTCTG CCCCTGAGGT TTTGCTAGTA AGAAAGCACA TGCGAGAGAG GCTGGCTGCT 
CTGGCAGCTC AGGCCTTCCC AGAAAGGCCC CATGAGAATG CACAGCTGGA ACTGGTCCTT 
GAAGTAGATG GGTTGCGGAG ATCGGTGCTC AATCTGGGTG CACTGCTCAC CACCAGTGCT 
GCTGCACACG AGACAGTGGC CACCGGCGAG GGCCTGCGCC AGGCACTGGT TGGCCAGCCT 
GCCTCACTCA CTGTCACCAC CAAAGACAAA GATGGGCGGC TGGTGCGCAC GGGCAGCGCA 
GAGCTGTGTG CAGAGATCAC TGGCCCCGAT GGCATGCGCC TTGCGGTACC GGTGGTGGAC 
CACAAAAATG GCACATATGA GCTGGTGTAC ACAGCACGTA CAGAAGGCGA CCTGCTCCTC 
TCAGTGCTGC TCTATGGACA GCCGGTGCGT GGCAGCCCTT TCCGTGTGCG TGCCCTGAGA 
CCCGGAGACT TGCCACCTTC CCCAGATGAT GTGAAGCGCC GGGTCAAGTC TCCCGGTGGT 
CCTGGCAGTC ATGTGCGCCA GAAGGCAGTG CGTAGGCCGA GCTCCATGTA CAGCACCGGT 
GGCAAACGGA AAGACAATCC AATTGTGGAT GAACTCGTCT TTCGTGTTGG CAGTCGTGGA 
AGGGAGAAAG GTGAATTCAC CAATTTACAC CCGCTGTCCG CTGCTAGCAG TGGCCGAATA 
GTGGTAGCAG ACAGCAACAA CCAATGTATT CAGGTTTTCT CCAATGAGGG CCAGTTCAAG 
TTCCGATTTG GAGTCCGTGG GCGCTCGCCT GGGCAACTAC AGCGTCCTAC GGGTGTGGCA 
GTGGACACCA ATGGAGACAT TATTGTGGCA GACTATGACA ACCGTTGGGT CAGCATCTTC 
TCTCCTGAGG GCAAGTTCAA GACCAAAATT GGAGCTGGCC GCCTCATGGG GCCCAAGGGA 
GTGGCTGTGG ACCGGAATGG GCATATCATT GTGGTGGATA ACAAGTCTTG CTGTGTCTTC 
ACCTTCCAGC CCAATGGCAA GCTTGTTGGG CGTTTTGGAG GCCGTGGGGC CACTGACCGC 
CACTTCGCAG GGCCCCACTT TGTGGCTGTG AACAACAAGA ATGAGATTGT AGTAACGGAT 
TTCCACAACC ATTCAGTGAA GGTGTACAGT GCTGATGGAG AGTTCCTCTT CAAATTTGGC 
TCACATGGCG AGGGCAATGG ACAGTTCAAT GCCCCCACGG GAGTAGCTGT GGATTCCAAT 
GGAAACATCA TCGTGGCTGA CTGGGGCAAT AGTCGCATAC AGGTATTCGA CAGCTCTGGT 
TCCTTCCTGT CCTATATCAA CACGTCTGCA GAGCCACTGT ATGGCCCACA GGGCTTGGCA 
TTGACCTCGG ATGGCCACGT AGTAGTGGCT GATGCTGGCA ACCACTGCTT TAAGGCCTAT 
CGATACCTCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The ability of TRIM3 to induce growth arrest depends on RING-dependent E3 ligase activity.
The Biochemical journal458(3)537-45(2014 Mar)
Raheja R,Liu Y,Hukkelhoven E,Yeh N,Koff A

Degradation of postsynaptic scaffold GKAP and regulation of dendritic spine morphology by the TRIM3 ubiquitin ligase in rat hippocampal neurons.
PloS one5(3)e9842(2010 Mar)
Hung AY,Sung CC,Brito IL,Sheng M

Involvement of myosin Vb in glutamate receptor trafficking.
The Journal of biological chemistry281(6)3669-78(2006 Feb)
Lis? MF,Wong TP,Trinh A,Hines RM,Liu L,Kang R,Hines DJ,Lu J,Goldenring JR,Wang YT,El-Husseini A

The tripartite motif family identifies cell compartments.
The EMBO journal20(9)2140-51(2001 May)
Reymond A,Meroni G,Fantozzi A,Merla G,Cairo S,Luzi L,Riganelli D,Zanaria E,Messali S,Cainarca S,Guffanti A,Minucci S,Pelicci PG,Ballabio A

BERP, a novel ring finger protein, binds to alpha-actinin-4.
Biochemical and biophysical research communications267(3)906-11(2000 Jan)
El-Husseini AE,Kwasnicka D,Yamada T,Hirohashi S,Vincent SR

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Trim3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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