Usf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usf1
Entrez Gene ID	83586
Full Name	upstream transcription factor 1
General protein information	Preferred Names upstream transcription factor 1 Names upstream stimulatory factor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q24
Summary	regulates the expression of matrix cytokine osteopontin (OPN); may play a role in cellular glucose metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006250266.2	XP_006250328.1	upstream stimulatory factor 1 isoform X1
XM_008769765.2	XP_008767987.1	upstream stimulatory factor 1 isoform X2
NM_031777.2	NP_113965.1	upstream stimulatory factor 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	usf1	NP_956590.1
Danio rerio (zebrafish)	usf1l	NP_998359.1
Bos taurus (cattle)	USF1	NP_001001161.1
Gallus gallus (chicken)	USF1	NP_001007486.1
Homo sapiens (human)	USF1	NP_009053.1
Mus musculus (house mouse)	Usf1	NP_033506.1
Rattus norvegicus (Norway rat)	Usf1	NP_113965.1
Pan troglodytes (chimpanzee)	USF1	XP_003949666.1
	USF1	XP_001117703.1
Canis lupus familiaris (dog)	USF1	XP_545763.2

Gene Ontology
Bibliography

Related articles in PubMed

Setd1a and NURF mediate chromatin dynamics and gene regulation during erythroid lineage commitment and differentiation.
Li Y, Schulz VP, Deng C, Li G, Shen Y, Tusi BK, Ma G, Stees J, Qiu Y, Steiner LA, Zhou L, Zhao K, Bungert J, Gallagher PG, Huang S
Nucleic acids research44(15)7173-88(2016 Sep)

Upstream stimulatory factor induces Nr5a1 and Shbg gene expression during the onset of rat Sertoli cell differentiation.
Wood MA, Mukherjee P, Toocheck CA, Walker WH
Biology of reproduction85(5)965-76(2011 Nov)

Hepatocyte growth factor family negatively regulates hepatic gluconeogenesis via induction of orphan nuclear receptor small heterodimer partner in primary hepatocytes.
Chanda D, Li T, Song KH, Kim YH, Sim J, Lee CH, Chiang JY, Choi HS
The Journal of biological chemistry284(42)28510-21(2009 Oct)

A role of DNA-PK for the metabolic gene regulation in response to insulin.
Wong RH, Chang I, Hudak CS, Hyun S, Kwan HY, Sul HS
Cell136(6)1056-72(2009 Mar)

Overexpression of USF increases TGF-beta1 protein levels, but G1 phase arrest was not induced in FRTL-5 cells.
Kim KS, Jung HS, Chung YJ, Jung TS, Jang HW, Lee MS, Kim KW, Chung JH
Journal of Korean medical science23(5)870-6(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Upstream stimulatory factors (USF1 and USF2) induce Nr5a1 and Shbg gene expression during the onset of rat Sertoli cell differentiation.
Title: Upstream stimulatory factor induces Nr5a1 and Shbg gene expression during the onset of rat Sertoli cell differentiation.

a novel signaling pathway inhibits hepatic gluconeogenesis through HGF/AMPK/USF-1/SHP
Title: Hepatocyte growth factor family negatively regulates hepatic gluconeogenesis via induction of orphan nuclear receptor small heterodimer partner in primary hepatocytes.

USF has a role in increasing TGF-beta1 protein levels, but not in indusing G1 phase arrest
Title: Overexpression of USF increases TGF-beta1 protein levels, but G1 phase arrest was not induced in FRTL-5 cells.

USF1 and USF2 are important contributors to neuronal-specific and mitogen-activated protein kinase regulation of the CGRP gene in trigeminal ganglion neurons
Title: Control of the calcitonin gene-related peptide enhancer by upstream stimulatory factor in trigeminal ganglion neurons.

Overexpression of upstream stimulatory factor 1 increases cyclic GMP-dependent protein kinase I promoter activity in human vascular smooth muscle cells.
Title: Upstream stimulatory factors (USF-1/USF-2) regulate human cGMP-dependent protein kinase I gene expression in vascular smooth muscle cells.

The following Usf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08710	NM_031777.2 Latest version!	Rattus norvegicus upstream transcription factor 1 (Usf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa08710	XM_006250266.2 Latest version!	Rattus norvegicus upstream transcription factor 1 (Usf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa35626	XM_008769765.2 Latest version!	Rattus norvegicus upstream transcription factor 1 (Usf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usf1 ( NM_031777.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113965.1 Rattus norvegicus upstream transcription factor 1 (Usf1), mRNA.

Price & Availability↑

CloneID	ORa08710
Clone ID Related Accession (Same CDS sequence)	XM_006250266.2 , NM_031777.2
Accession Version	NM_031777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	upstream stimulatory factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088849.1. On Jan 12, 2005 this sequence version replaced NM_031777.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC088849.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGGGC AGCAGAAAAC AGCCGAAACC GAAGAGGGGA CAGTGCAGAT TCAGGAAGGT 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA ATCAGCTGCC 
ACTTTCCCCG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC TGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCT 
ATCAGTGGCT ACCCTGCCAC TCAGTCTATG ACTCAGGCAG TAATCCAGGG GGCTTTCACC 
AGTGATGATG CTGTTGACGC GGAGGGAACA GCTGCCGAGA CCCACTACAC TTACTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACGACATCTG GGAGTACAAC CGCCGTGGTC 
ACCACCCAGG GCTCAGAGGC ACTGCTGGGG CAGGCAACCC CGCCCAGCAC AGGCCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTGTTA CAGGGAGGGA GCCAGCGGTC AATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACGCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAG 
GGTGGAATCC TGTCCAAAGC CTGTGACTAT ATCCAGGAAC TACGTCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTCG ACAATGACGT GCTTCGACAG 
CAGGTGGAAG ATCTTAAAAA CAAGAACCTG CTACTACGAG CTCAGTTACG GCACCACGGA 
TTAGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113965.1
CDS	132..1064
Translation	MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDAEGTAAETHYTYFPSTAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

Target ORF information:

RefSeq Version	NM_031777.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus upstream transcription factor 1 (Usf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031777.2

ORF Insert Sequence:

ATGAAGGGGC AGCAGAAAAC AGCCGAAACC GAAGAGGGGA CAGTGCAGAT TCAGGAAGGT 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA ATCAGCTGCC 
ACTTTCCCCG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC TGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCT 
ATCAGTGGCT ACCCTGCCAC TCAGTCTATG ACTCAGGCAG TAATCCAGGG GGCTTTCACC 
AGTGATGATG CTGTTGACGC GGAGGGAACA GCTGCCGAGA CCCACTACAC TTACTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACGACATCTG GGAGTACAAC CGCCGTGGTC 
ACCACCCAGG GCTCAGAGGC ACTGCTGGGG CAGGCAACCC CGCCCAGCAC AGGCCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTGTTA CAGGGAGGGA GCCAGCGGTC AATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACGCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAG 
GGTGGAATCC TGTCCAAAGC CTGTGACTAT ATCCAGGAAC TACGTCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTCG ACAATGACGT GCTTCGACAG 
CAGGTGGAAG ATCTTAAAAA CAAGAACCTG CTACTACGAG CTCAGTTACG GCACCACGGA 
TTAGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf1 ( XM_006250266.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250328.1 Rattus norvegicus upstream transcription factor 1 (Usf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa08710
Clone ID Related Accession (Same CDS sequence)	XM_006250266.2 , NM_031777.2
Accession Version	XM_006250266.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	upstream stimulatory factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006250266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGGC AGCAGAAAAC AGCCGAAACC GAAGAGGGGA CAGTGCAGAT TCAGGAAGGT 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA ATCAGCTGCC 
ACTTTCCCCG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC TGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCT 
ATCAGTGGCT ACCCTGCCAC TCAGTCTATG ACTCAGGCAG TAATCCAGGG GGCTTTCACC 
AGTGATGATG CTGTTGACGC GGAGGGAACA GCTGCCGAGA CCCACTACAC TTACTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACGACATCTG GGAGTACAAC CGCCGTGGTC 
ACCACCCAGG GCTCAGAGGC ACTGCTGGGG CAGGCAACCC CGCCCAGCAC AGGCCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTGTTA CAGGGAGGGA GCCAGCGGTC AATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACGCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAG 
GGTGGAATCC TGTCCAAAGC CTGTGACTAT ATCCAGGAAC TACGTCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTCG ACAATGACGT GCTTCGACAG 
CAGGTGGAAG ATCTTAAAAA CAAGAACCTG CTACTACGAG CTCAGTTACG GCACCACGGA 
TTAGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250328.1
CDS	317..1249
Translation	MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDAEGTAAETHYTYFPSTAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

Target ORF information:

RefSeq Version	XM_006250266.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus upstream transcription factor 1 (Usf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250266.2

ORF Insert Sequence:

ATGAAGGGGC AGCAGAAAAC AGCCGAAACC GAAGAGGGGA CAGTGCAGAT TCAGGAAGGT 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA ATCAGCTGCC 
ACTTTCCCCG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC TGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCT 
ATCAGTGGCT ACCCTGCCAC TCAGTCTATG ACTCAGGCAG TAATCCAGGG GGCTTTCACC 
AGTGATGATG CTGTTGACGC GGAGGGAACA GCTGCCGAGA CCCACTACAC TTACTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACGACATCTG GGAGTACAAC CGCCGTGGTC 
ACCACCCAGG GCTCAGAGGC ACTGCTGGGG CAGGCAACCC CGCCCAGCAC AGGCCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTGTTA CAGGGAGGGA GCCAGCGGTC AATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACGCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAG 
GGTGGAATCC TGTCCAAAGC CTGTGACTAT ATCCAGGAAC TACGTCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTCG ACAATGACGT GCTTCGACAG 
CAGGTGGAAG ATCTTAAAAA CAAGAACCTG CTACTACGAG CTCAGTTACG GCACCACGGA 
TTAGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf1 ( XM_008769765.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767987.1 Rattus norvegicus upstream transcription factor 1 (Usf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa35626
Clone ID Related Accession (Same CDS sequence)	XM_008769765.2
Accession Version	XM_008769765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	upstream stimulatory factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGG TGATCCAGGT GTCTGAGGGG CAGCTGGATG GCCAGACAGA GGGCTCTGGC 
GCTATCAGTG GCTACCCTGC CACTCAGTCT ATGACTCAGG CAGTAATCCA GGGGGCTTTC 
ACCAGTGATG ATGCTGTTGA CGCGGAGGGA ACAGCTGCCG AGACCCACTA CACTTACTTC 
CCCAGCACCG CAGTGGGAGA TGGGTCAGGG GGTACGACAT CTGGGAGTAC AACCGCCGTG 
GTCACCACCC AGGGCTCAGA GGCACTGCTG GGGCAGGCAA CCCCGCCCAG CACAGGCCAA 
TTCTTTGTGA TGATGTCACC ACAAGAAGTG TTACAGGGAG GGAGCCAGCG GTCAATTGCC 
CCCAGGACCC ACCCTTATTC CCCGAAGTCA GAGGCTCCCA GGACAACGCG AGATGAGAAA 
CGGAGGGCTC AACATAACGA AGTGGAGCGC CGCCGCCGGG ACAAGATCAA CAACTGGATT 
GTACAGCTGT CCAAAATCAT CCCAGACTGC TCTATGGAGA GCACCAAGTC TGGCCAGAGT 
AAGGGTGGAA TCCTGTCCAA AGCCTGTGAC TATATCCAGG AACTACGTCA GAGCAACCAC 
CGGCTGTCTG AAGAGCTGCA GGGGTTAGAT CAGTTGCAGC TCGACAATGA CGTGCTTCGA 
CAGCAGGTGG AAGATCTTAA AAACAAGAAC CTGCTACTAC GAGCTCAGTT ACGGCACCAC 
GGATTAGAGG TCGTCATCAA GAATGACAGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767987.1
CDS	229..984
Translation	MYRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDAEGTAAETHYTYFPSTAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

Target ORF information:

RefSeq Version	XM_008769765.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus upstream transcription factor 1 (Usf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769765.2

ORF Insert Sequence:

ATGTACAGGG TGATCCAGGT GTCTGAGGGG CAGCTGGATG GCCAGACAGA GGGCTCTGGC 
GCTATCAGTG GCTACCCTGC CACTCAGTCT ATGACTCAGG CAGTAATCCA GGGGGCTTTC 
ACCAGTGATG ATGCTGTTGA CGCGGAGGGA ACAGCTGCCG AGACCCACTA CACTTACTTC 
CCCAGCACCG CAGTGGGAGA TGGGTCAGGG GGTACGACAT CTGGGAGTAC AACCGCCGTG 
GTCACCACCC AGGGCTCAGA GGCACTGCTG GGGCAGGCAA CCCCGCCCAG CACAGGCCAA 
TTCTTTGTGA TGATGTCACC ACAAGAAGTG TTACAGGGAG GGAGCCAGCG GTCAATTGCC 
CCCAGGACCC ACCCTTATTC CCCGAAGTCA GAGGCTCCCA GGACAACGCG AGATGAGAAA 
CGGAGGGCTC AACATAACGA AGTGGAGCGC CGCCGCCGGG ACAAGATCAA CAACTGGATT 
GTACAGCTGT CCAAAATCAT CCCAGACTGC TCTATGGAGA GCACCAAGTC TGGCCAGAGT 
AAGGGTGGAA TCCTGTCCAA AGCCTGTGAC TATATCCAGG AACTACGTCA GAGCAACCAC 
CGGCTGTCTG AAGAGCTGCA GGGGTTAGAT CAGTTGCAGC TCGACAATGA CGTGCTTCGA 
CAGCAGGTGG AAGATCTTAA AAACAAGAAC CTGCTACTAC GAGCTCAGTT ACGGCACCAC 
GGATTAGAGG TCGTCATCAA GAATGACAGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Setd1a and NURF mediate chromatin dynamics and gene regulation during erythroid lineage commitment and differentiation.
Nucleic acids research44(15)7173-88(2016 Sep)
Li Y,Schulz VP,Deng C,Li G,Shen Y,Tusi BK,Ma G,Stees J,Qiu Y,Steiner LA,Zhou L,Zhao K,Bungert J,Gallagher PG,Huang S

Upstream stimulatory factor induces Nr5a1 and Shbg gene expression during the onset of rat Sertoli cell differentiation.
Biology of reproduction85(5)965-76(2011 Nov)
Wood MA,Mukherjee P,Toocheck CA,Walker WH

Hepatocyte growth factor family negatively regulates hepatic gluconeogenesis via induction of orphan nuclear receptor small heterodimer partner in primary hepatocytes.
The Journal of biological chemistry284(42)28510-21(2009 Oct)
Chanda D,Li T,Song KH,Kim YH,Sim J,Lee CH,Chiang JY,Choi HS

A role of DNA-PK for the metabolic gene regulation in response to insulin.
Cell136(6)1056-72(2009 Mar)
Wong RH,Chang I,Hudak CS,Hyun S,Kwan HY,Sul HS

Overexpression of USF increases TGF-beta1 protein levels, but G1 phase arrest was not induced in FRTL-5 cells.
Journal of Korean medical science23(5)870-6(2008 Oct)
Kim KS,Jung HS,Chung YJ,Jung TS,Jang HW,Lee MS,Kim KW,Chung JH

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Usf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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