gRNAs
The following Polr1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa61024 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008762998.2 | |
| Accession Version | XM_008762998.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | DNA-directed RNA polymerase I subunit RPA1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGGCCT CCAAGCACAC GCCATGGCGG CGGCTTCAGG GCATTTCCTT CGGGATGTAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008761220.2 |
| CDS | 338..5581 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_008762998.2 |
1 | ATGCTGGCCT CCAAGCACAC GCCATGGCGG CGGCTTCAGG GCATTTCCTT CGGGATGTAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa08695 | |
| Clone ID Related Accession (Same CDS sequence) | NM_031772.1 | |
| Accession Version | NM_031772.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-26 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | DNA-directed RNA polymerase I subunit RPA1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF025425.1. ##Evidence-Data-START## Transcript exon combination :: AF025425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGCTGGCCT CCAAGCACAC GCCATGGCGG CGGCTTCAGG GCATTTCCTT CGGGATGTAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_113960.1 |
| CDS | 57..5207 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_031772.1 |
1 | ATGCTGGCCT CCAAGCACAC GCCATGGCGG CGGCTTCAGG GCATTTCCTT CGGGATGTAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The Warsaw breakage syndrome-related protein DDX11 is required for ribosomal RNA synthesis and embryonic development. |
CRM1 and its ribosome export adaptor NMD3 localize to the nucleolus and affect rRNA synthesis. |
Conservation between the RNA polymerase I, II, and III transcription initiation machineries. |
RNA polymerase I activity is regulated at multiple steps in the transcription cycle: recent insights into factors that influence transcription elongation. |
Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription. |
