Gnb5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnb5
Entrez Gene ID	83579
Full Name	G protein subunit beta 5
General protein information	Preferred Names G protein subunit beta 5 Names guanine nucleotide-binding protein subunit beta-5 gbeta5 guanine nucleotide binding protein (G protein), beta 5 guanine nucleotide binding protein (G protein), beta polypeptide 5 guanine nucleotide binding protein, beta 5 guanine nucleotide-binding protein, beta-5 subunit transducin beta chain 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24
Summary	beta subunit of a heterotrimeric G-protein that is required for G-protein GTPase activity; involved as a modulator or transducer in a variety of transmembrane signalling systems [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017595933.1	XP_017451422.1	guanine nucleotide-binding protein subunit beta-5 isoform X1
NM_031770.3	NP_113958.1	guanine nucleotide-binding protein subunit beta-5

Pathways from BioSystems

Homologies

Homo sapiens (human)	GNB5	NP_057278.2
Rattus norvegicus (Norway rat)	Gnb5	NP_113958.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012525	XP_312729.4
Drosophila melanogaster (fruit fly)	Gbeta5	NP_572452.1
Caenorhabditis elegans (roundworm)	gpb-2	NP_492072.1
Pan troglodytes (chimpanzee)	GNB5	XP_001151191.3
Canis lupus familiaris (dog)	GNB5	XP_005638744.1
Mus musculus (house mouse)	Gnb5	NP_034443.1
Eremothecium gossypii	AGOS_ACR097W	NP_983499.1
Xenopus tropicalis (tropical clawed frog)	gnb5	NP_001073035.1
Bos taurus (cattle)	GNB5	NP_001092540.1
Gallus gallus (chicken)	GNB5	XP_413801.2
Danio rerio (zebrafish)	gnb5b	NP_957040.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010318	XP_320124.6
Saccharomyces cerevisiae (baker's yeast)	STE4	NP_014855.3
Kluyveromyces lactis	KLLA0D06787g	XP_453363.1

Gene Ontology
Bibliography

Related articles in PubMed

GNB5 mutation causes a novel neuropsychiatric disorder featuring attention deficit hyperactivity disorder, severely impaired language development and normal cognition.
Shamseldin HE, Masuho I, Alenizi A, Alyamani S, Patil DN, Ibrahim N, Martemyanov KA, Alkuraya FS
Genome biology17(1)195(2016 Sep)

Cellular and Subcellular Localization of the RGS7/G?5/R7BP Complex in the Cerebellar Cortex.
Aguado C, Orlandi C, Fajardo-Serrano A, Gil-Minguez M, Martemyanov KA, Luj?n R
Frontiers in neuroanatomy10114(2016)

Light-induced translocation of RGS9-1 and G?5L in mouse rod photoreceptors.
Tian M, Zallocchi M, Wang W, Chen CK, Palczewski K, Delimont D, Cosgrove D, Peng YW
PloS one8(3)e58832(2013)

Photoreceptor signaling: supporting vision across a wide range of light intensities.
Arshavsky VY, Burns ME
The Journal of biological chemistry287(3)1620-6(2012 Jan)

Role of molecular chaperones in G protein beta5/regulator of G protein signaling dimer assembly and G protein betagamma dimer specificity.
Howlett AC, Gray AJ, Hunter JM, Willardson BM
The Journal of biological chemistry284(24)16386-99(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that G protein inhibition of N-type calcium channels is critically dependent on two separate but adjacent approximately 20-amino acid regions of the Gbeta subunit, as examined with Gbetas 1 and 5 and Ggamma2.
Title: A single Gbeta subunit locus controls cross-talk between protein kinase C and G protein regulation of N-type calcium channels.

The following Gnb5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnb5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08692	NM_031770.3 Latest version!	Rattus norvegicus G protein subunit beta 5 (Gnb5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa62563	XM_017595933.1 Latest version!	Rattus norvegicus G protein subunit beta 5 (Gnb5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gnb5 ( NM_031770.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113958.1 Rattus norvegicus G protein subunit beta 5 (Gnb5), mRNA.

Price & Availability↑

CloneID	ORa08692
Clone ID Related Accession (Same CDS sequence)	NM_031770.3
Accession Version	NM_031770.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein subunit beta-5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY552803.1. On Jun 10, 2007 this sequence version replaced NM_031770.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY552803.1, BC089221.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCAACCG ATGGGCTGCA CGAGAACGAG ACGCTGGCGT CGCTGAAGAG CGAGGCCGAG 
AGCCTCAAGG GCAAGCTAGA GGAGGAGCGG GCGAAGCTGC ATGACGTGGA GCTTCACCAG 
GTGGCCGAGC GGGTGGAGGC CCTGGGCCAG TTTGTCATGA AGACCAGAAG GACCCTCAAA 
GGACACGGGA ACAAAGTCCT GTGCATGGAT TGGTGCAAAG ACAAGCGGAG GATCGTGAGC 
TCCTCACAGG ATGGGAAGGT GATTGTCTGG GATTCCTTCA CTACTAACAA GGAGCACGCG 
GTCACCATGC CTTGCACCTG GGTGATGGCG TGTGCTTATG CCCCATCAGG ATGTGCCATT 
GCTTGTGGTG GTCTGGATAA TAAGTGTTCC GTGTACCCAC TGACATTTGA CAAGAACGAG 
AACATGGCTG CCAAGAAGAA GTCTGTCGCT ATGCACACCA ACTACCTGTC TGCCTGCAGC 
TTCACCAACT CAGACATGCA GATCCTGACA GCCAGCGGTG ATGGCACTTG CGCCCTGTGG 
GATGTGGAAA GTGGACAGCT GTTGCAGAGC TTCCATGGCC ATGGGGCTGA CGTCCTCTGC 
TTGGACCTGG CCCCCTCAGA AACTGGAAAC ACCTTCGTGT CTGGGGGTTG TGACAAGAAG 
GCCATGGTGT GGGACATGCG CTCTGGCCAG TGTGTGCAAG CCTTTGAAAC TCATGAATCT 
GATGTCAACA GTGTCCGATA CTACCCCAGT GGAGATGCTT TTGCTTCCGG ATCAGATGAT 
GCCACGTGTC GCCTCTATGA CCTGAGGGCA GATAGAGAGG TTGCCATCTA CTCCAAGGAG 
AGTATTATAT TTGGAGCATC AAGCGTGGAT TTCTCCCTCA GTGGCCGCCT GCTCTTTGCT 
GGGTACAATG ACTATACCAT CAATGTCTGG GATGTTCTCA AGGGCTCCCG GGTCTCCATC 
CTGTTTGGAC ATGAAAACCG TGTTAGCACT CTGCGAGTGT CTCCTGATGG GACAGCATTC 
TGCTCAGGGT CGTGGGATCA CACCCTAAGA GTTTGGGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113958.1
CDS	1..1062
Translation	MATDGLHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRRTLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACGGLDNKCSVYPLTFDKNENMAAKKKSVAMHTNYLSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCVQAFETHESDVNSVRYYPSGDAFASGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRVWA

Target ORF information:

RefSeq Version	NM_031770.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit beta 5 (Gnb5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031770.3

ORF Insert Sequence:

ATGGCAACCG ATGGGCTGCA CGAGAACGAG ACGCTGGCGT CGCTGAAGAG CGAGGCCGAG 
AGCCTCAAGG GCAAGCTAGA GGAGGAGCGG GCGAAGCTGC ATGACGTGGA GCTTCACCAG 
GTGGCCGAGC GGGTGGAGGC CCTGGGCCAG TTTGTCATGA AGACCAGAAG GACCCTCAAA 
GGACACGGGA ACAAAGTCCT GTGCATGGAT TGGTGCAAAG ACAAGCGGAG GATCGTGAGC 
TCCTCACAGG ATGGGAAGGT GATTGTCTGG GATTCCTTCA CTACTAACAA GGAGCACGCG 
GTCACCATGC CTTGCACCTG GGTGATGGCG TGTGCTTATG CCCCATCAGG ATGTGCCATT 
GCTTGTGGTG GTCTGGATAA TAAGTGTTCC GTGTACCCAC TGACATTTGA CAAGAACGAG 
AACATGGCTG CCAAGAAGAA GTCTGTCGCT ATGCACACCA ACTACCTGTC TGCCTGCAGC 
TTCACCAACT CAGACATGCA GATCCTGACA GCCAGCGGTG ATGGCACTTG CGCCCTGTGG 
GATGTGGAAA GTGGACAGCT GTTGCAGAGC TTCCATGGCC ATGGGGCTGA CGTCCTCTGC 
TTGGACCTGG CCCCCTCAGA AACTGGAAAC ACCTTCGTGT CTGGGGGTTG TGACAAGAAG 
GCCATGGTGT GGGACATGCG CTCTGGCCAG TGTGTGCAAG CCTTTGAAAC TCATGAATCT 
GATGTCAACA GTGTCCGATA CTACCCCAGT GGAGATGCTT TTGCTTCCGG ATCAGATGAT 
GCCACGTGTC GCCTCTATGA CCTGAGGGCA GATAGAGAGG TTGCCATCTA CTCCAAGGAG 
AGTATTATAT TTGGAGCATC AAGCGTGGAT TTCTCCCTCA GTGGCCGCCT GCTCTTTGCT 
GGGTACAATG ACTATACCAT CAATGTCTGG GATGTTCTCA AGGGCTCCCG GGTCTCCATC 
CTGTTTGGAC ATGAAAACCG TGTTAGCACT CTGCGAGTGT CTCCTGATGG GACAGCATTC 
TGCTCAGGGT CGTGGGATCA CACCCTAAGA GTTTGGGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnb5 ( XM_017595933.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451422.1 Rattus norvegicus G protein subunit beta 5 (Gnb5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62563
Clone ID Related Accession (Same CDS sequence)	XM_017595933.1
Accession Version	XM_017595933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein subunit beta-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) and transcript sequence (NM_031770.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 95 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGTGTGCA CATACACATC AATAAAAAGC CTCATCTCGA CCAAGATGTG CGATCAGACC 
TTCCTGGTTA ACGTGTTTGG CTCATGTGAC AAATGCTTCA AACAAAGAGC CCTACGGCCC 
GTCTTCAAGA AATCTCAACA ACTCAACTAC TGCTCCCCAT GTGCAGAGAT CATGGCAACC 
GATGGGCTGC ACGAGAACGA GACGCTGGCG TCGCTGAAGA GCGAGGCCGA GAGCCTCAAG 
GGCAAGCTAG AGGAGGAGCG GGCGAAGCTG CATGACGTGG AGCTTCACCA GGTGGCCGAG 
CGGGTGGAGG CCCTGGGCCA GTTTGTCATG AAGACCAGAA GGACCCTCAA AGGACACGGG 
AACAAAGTCC TGTGCATGGA TTGGTGCAAA GACAAGCGGA GGATCGTGAG CTCCTCACAG 
GATGGGAAGG TGATTGTCTG GGATTCCTTC ACTACTAACA AGGAGCACGC GGTCACCATG 
CCTTGCACCT GGGTGATGGC GTGTGCTTAT GCCCCATCAG GATGTGCCAT TGCTTGTGGT 
GGTCTGGATA ATAAGTGTTC CGTGTACCCA CTGACATTTG ACAAGAACGA GAACATGGCT 
GCCAAGAAGA AGTCTGTCGC TATGCACACC AACTACCTGT CTGCCTGCAG CTTCACCAAC 
TCAGACATGC AGATCCTGAC AGCCAGCGGT GATGGCACTT GCGCCCTGTG GGATGTGGAA 
AGTGGACAGC TGTTGCAGAG CTTCCATGGC CATGGGGCTG ACGTCCTCTG CTTGGACCTG 
GCCCCCTCAG AAACTGGAAA CACCTTCGTG TCTGGGGGTT GTGACAAGAA GGCCATGGTG 
TGGGACATGC GCTCTGGCCA GTGTGTGCAA GCCTTTGAAA CTCATGAATC TGATGTCAAC 
AGTGTCCGAT ACTACCCCAG TGGAGATGCT TTTGCTTCCG GATCAGATGA TGCCACGTGT 
CGCCTCTATG ACCTGAGGGC AGATAGAGAG GTTGCCATCT ACTCCAAGGA GAGTATTATA 
TTTGGAGCAT CAAGCGTGGA TTTCTCCCTC AGTGGCCGCC TGCTCTTTGC TGGGTACAAT 
GACTATACCA TCAATGTCTG GGATGTTCTC AAGGGCTCCC GGGTCTCCAT CCTGTTTGGA 
CATGAAAACC GTGTTAGCAC TCTGCGAGTG TCTCCTGATG GGACAGCATT CTGCTCAGGG 
TCGTGGGATC ACACCCTAAG AGTTTGGGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451422.1
CDS	448..1680
Translation	MVCTYTSIKSLISTKMCDQTFLVNVFGSCDKCFKQRALRPVFKKSQQLNYCSPCAEIMATDGLHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRRTLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACGGLDNKCSVYPLTFDKNENMAAKKKSVAMHTNYLSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCVQAFETHESDVNSVRYYPSGDAFASGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRVWA

Target ORF information:

RefSeq Version	XM_017595933.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit beta 5 (Gnb5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595933.1

ORF Insert Sequence:

ATGGTGTGCA CATACACATC AATAAAAAGC CTCATCTCGA CCAAGATGTG CGATCAGACC 
TTCCTGGTTA ACGTGTTTGG CTCATGTGAC AAATGCTTCA AACAAAGAGC CCTACGGCCC 
GTCTTCAAGA AATCTCAACA ACTCAACTAC TGCTCCCCAT GTGCAGAGAT CATGGCAACC 
GATGGGCTGC ACGAGAACGA GACGCTGGCG TCGCTGAAGA GCGAGGCCGA GAGCCTCAAG 
GGCAAGCTAG AGGAGGAGCG GGCGAAGCTG CATGACGTGG AGCTTCACCA GGTGGCCGAG 
CGGGTGGAGG CCCTGGGCCA GTTTGTCATG AAGACCAGAA GGACCCTCAA AGGACACGGG 
AACAAAGTCC TGTGCATGGA TTGGTGCAAA GACAAGCGGA GGATCGTGAG CTCCTCACAG 
GATGGGAAGG TGATTGTCTG GGATTCCTTC ACTACTAACA AGGAGCACGC GGTCACCATG 
CCTTGCACCT GGGTGATGGC GTGTGCTTAT GCCCCATCAG GATGTGCCAT TGCTTGTGGT 
GGTCTGGATA ATAAGTGTTC CGTGTACCCA CTGACATTTG ACAAGAACGA GAACATGGCT 
GCCAAGAAGA AGTCTGTCGC TATGCACACC AACTACCTGT CTGCCTGCAG CTTCACCAAC 
TCAGACATGC AGATCCTGAC AGCCAGCGGT GATGGCACTT GCGCCCTGTG GGATGTGGAA 
AGTGGACAGC TGTTGCAGAG CTTCCATGGC CATGGGGCTG ACGTCCTCTG CTTGGACCTG 
GCCCCCTCAG AAACTGGAAA CACCTTCGTG TCTGGGGGTT GTGACAAGAA GGCCATGGTG 
TGGGACATGC GCTCTGGCCA GTGTGTGCAA GCCTTTGAAA CTCATGAATC TGATGTCAAC 
AGTGTCCGAT ACTACCCCAG TGGAGATGCT TTTGCTTCCG GATCAGATGA TGCCACGTGT 
CGCCTCTATG ACCTGAGGGC AGATAGAGAG GTTGCCATCT ACTCCAAGGA GAGTATTATA 
TTTGGAGCAT CAAGCGTGGA TTTCTCCCTC AGTGGCCGCC TGCTCTTTGC TGGGTACAAT 
GACTATACCA TCAATGTCTG GGATGTTCTC AAGGGCTCCC GGGTCTCCAT CCTGTTTGGA 
CATGAAAACC GTGTTAGCAC TCTGCGAGTG TCTCCTGATG GGACAGCATT CTGCTCAGGG 
TCGTGGGATC ACACCCTAAG AGTTTGGGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GNB5 mutation causes a novel neuropsychiatric disorder featuring attention deficit hyperactivity disorder, severely impaired language development and normal cognition.
Genome biology17(1)195(2016 Sep)
Shamseldin HE,Masuho I,Alenizi A,Alyamani S,Patil DN,Ibrahim N,Martemyanov KA,Alkuraya FS

Cellular and Subcellular Localization of the RGS7/G?5/R7BP Complex in the Cerebellar Cortex.
Frontiers in neuroanatomy10114(2016)
Aguado C,Orlandi C,Fajardo-Serrano A,Gil-Minguez M,Martemyanov KA,Luj?n R

Light-induced translocation of RGS9-1 and G?5L in mouse rod photoreceptors.
PloS one8(3)e58832(2013)
Tian M,Zallocchi M,Wang W,Chen CK,Palczewski K,Delimont D,Cosgrove D,Peng YW

Photoreceptor signaling: supporting vision across a wide range of light intensities.
The Journal of biological chemistry287(3)1620-6(2012 Jan)
Arshavsky VY,Burns ME

Role of molecular chaperones in G protein beta5/regulator of G protein signaling dimer assembly and G protein betagamma dimer specificity.
The Journal of biological chemistry284(24)16386-99(2009 Jun)
Howlett AC,Gray AJ,Hunter JM,Willardson BM

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Gnb5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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