Smpd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smpd2
Entrez Gene ID	83537
Full Name	sphingomyelin phosphodiesterase 2
Synonyms	nSMase
General protein information	Preferred Names sphingomyelin phosphodiesterase 2 Names sphingomyelin phosphodiesterase 2 N-SMase lyso-PAF-PLC lyso-platelet-activating factor-phospholipase C neutral sphingomyelinase sphingomyelin phosphodiesterase 2, neutral membrane
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q12
Summary	catalyzes the Mg2+ dependent hydrolysis of sphingomyelin to form N-acylsphingosine and choline phosphate; also has a lower activity for the hydrolysis of lyso-platelet activating factor to 1-alkylglycerol [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031360.2	NP_112650.1	sphingomyelin phosphodiesterase 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	smpd2a	NP_001025390.1
Danio rerio (zebrafish)	LOC797914	XP_001338369.2
Pan troglodytes (chimpanzee)	SMPD2	XP_001152001.1
Rattus norvegicus (Norway rat)	Smpd2	NP_112650.1
Gallus gallus (chicken)	SMPD2	XP_001232516.1
Caenorhabditis elegans (roundworm)	T27F6.6	NP_493169.2
	SMPD2	XP_001090569.1
Drosophila melanogaster (fruit fly)	CG12034	NP_647790.1
Xenopus tropicalis (tropical clawed frog)	smpd2	NP_001120209.1
Homo sapiens (human)	SMPD2	NP_003071.2
Bos taurus (cattle)	SMPD2	NP_001068851.2
Mus musculus (house mouse)	Smpd2	NP_033239.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008053	XP_001689028.1
Canis lupus familiaris (dog)	SMPD2	XP_005627754.1

Gene Ontology
Bibliography

Related articles in PubMed

Neutral sphingomyelinase inhibition alleviates apoptosis, but not ER stress, in liver ischemia-reperfusion injury.
Tuzcu H, Unal B, K?rac E, Konuk E, Ozcan F, Elpek GO, Demir N, Aslan M
Free radical research51(3)253-268(2017 Mar)

Involvement of neutral sphingomyelinase in the angiotensin II signaling pathway.
Bautista-P?rez R, del Valle-Mondrag?n L, Cano-Mart?nez A, P?rez-M?ndez O, Escalante B, Franco M
American journal of physiology. Renal physiology308(10)F1178-87(2015 May)

Trk receptors need neutral sphingomyelinase activity to promote cell viability.
Candalija A, Cub? R, Ortega A, Aguilera J, Gil C
FEBS letters588(1)167-74(2014 Jan)

Endocytosis and intracellular processing of BODIPY-sphingomyelin by murine CATH.a neurons.
Nusshold C, Uellen A, Bernhart E, Hammer A, Damm S, Wintersperger A, Reicher H, Hermetter A, Malle E, Sattler W
Biochimica et biophysica acta1831(12)1665-78(2013 Dec)

Endothelin-1 stimulates small artery VCAM-1 expression through p38MAPK-dependent neutral sphingomyelinase.
Ohanian J, Forman SP, Katzenberg G, Ohanian V
Journal of vascular research49(4)353-62(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data herein confirm the role of ceramide in increased apoptotic cell death and highlight the protective effect of N-SMase inhibition in down-regulation of apoptotic stimuli responses occurring in hepatic ischemia reperfusion injury.
Title: Neutral sphingomyelinase inhibition alleviates apoptosis, but not ER stress, in liver ischemia-reperfusion injury.

ANG II stimulates ceramide formation via the activation of nSMase in the kidney.
Title: Involvement of neutral sphingomyelinase in the angiotensin II signaling pathway.

Data suggest that neurotrophins can promote cell viability through Trk receptors in a manner depending on basal neutral sphingomyelinase (nSMase) but not through SMase activity enhancement.
Title: Trk receptors need neutral sphingomyelinase activity to promote cell viability.

results indicate that endothelin-1 stimulates arterial VCAM-1 expression through p38MAPK-dependent activation of neutral sphingomyelinases
Title: Endothelin-1 stimulates small artery VCAM-1 expression through p38MAPK-dependent neutral sphingomyelinase.

nSMase-derived ceramide production and the activation of PKCzeta are early and necessary events in the signalling cascade of acute hypoxic pulmonary vasoconstriction.
Title: Activation of neutral sphingomyelinase is involved in acute hypoxic pulmonary vasoconstriction.

The following Smpd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smpd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12041	NM_031360.2 Latest version!	Rattus norvegicus sphingomyelin phosphodiesterase 2 (Smpd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smpd2 ( NM_031360.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112650.1 Rattus norvegicus sphingomyelin phosphodiesterase 2 (Smpd2), mRNA.

Price & Availability↑

CloneID	ORa12041
Clone ID Related Accession (Same CDS sequence)	NM_031360.2
Accession Version	NM_031360.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	sphingomyelin phosphodiesterase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC091139.1. On Mar 27, 2005 this sequence version replaced NM_031360.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC091139.1, AB047002.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCACA ACTTTTCTCT GCGGCTGAGG GTTTTCAACC TCAACTGCTG GGACATCCCC 
TACCTAAGCA AGCATAGGGC CGACCGCATG AAGCGCTTGG GAGACTTTCT GAACTTGGAG 
AGCTTTGACC TGGCTCTGCT GGAGGAGGTG TGGAGTGAGC AGGACTTCCA GTACTTAAAG 
CAAAAGCTAT CGCTCACCTA CCCGGATGCA CACTACTTCA GAAGCGGAAT CATTGGCAGT 
GGCCTCTGTG TGTTCTCCAG ACACCCAATC CAGGAAATTG TCCAGCACGT CTATACTCTC 
AATGGTTACC CCTACAAGTT CTATCATGGA GACTGGTTCT GTGGGAAGGC TGTGGGGCTG 
CTGGTGCTCC ATCTAAGCGG ACTGGTGCTC AACGCCTACG TGACCCATCT GCACGCTGAG 
TACAGCCGGC AGAAGGATAT CTACTTCGCA CACCGTGTGG CCCAAGCTTG GGAACTGGCC 
CAGTTCATCC ACCACACATC CAAGAAAGCA AATGTGGTCC TGTTGTGCGG GGATCTCAAC 
ATGCACCCCA AAGACCTGGG CTGCTGCCTA CTGAAAGAGT GGACGGGGCT CCGTGATGCC 
TTTGTTGAGA CCGAGGACTT TAAGGGCTCA GAGGACGGCT GTACCATGGT ACCCAAGAAC 
TGCTACGTCA GCCAGCAGGA CCTGGGGCCG TTTCCCTTTG GCGTCCGTAT CGACTACGTG 
CTTTACAAGG CAGTCTCTGG CTTCCACATC TGCTGTAAGA CTCTGAAAAC CACTACGGGC 
TGTGACCCCC ACAATGGCAC CCCCTTCTCT GACCACGAGG CCCTCATGGC TACTTTGTGT 
GTGAAGCACA GCCCCCCTCA GGAAGACCCT TGCTCTGCCC ATGGATCAGC GGAAAGGTCA 
GCATTGATCA GCGCGCTAAG GGAGGCGAGG ACAGAGCTGG GGCGAGGCAT AGCTCAAGCT 
CGCTGGTGGG CTGCACTCTT TGGCTATGTG ATGATTTTGG GGCTGTCCCT CCTGGTGTTG 
CTGTGTGTCC TGGCTGCAGG AGAAGAGGCC AGGGAAGTGG CCATAATGCT CTGGACACCC 
AGTGTGGGTC TGGTGCTGGG GGCAGGCGCA GTCTACCTGT TCCACAAGCA GGAGGCCAAA 
AGCTTATGTC GGGCCCAGGC TGAGATACAA CATGTTCTGA CAAGGACAAC GGAGACCCAG 
GACCTAGGCT CAGAGCCTCA CCCAACCCAC TGCCGGCAGC AGGAGGCGGA CAGAGCTGAA 
GAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112650.1
CDS	262..1530
Translation	MKHNFSLRLRVFNLNCWDIPYLSKHRADRMKRLGDFLNLESFDLALLEEVWSEQDFQYLKQKLSLTYPDAHYFRSGIIGSGLCVFSRHPIQEIVQHVYTLNGYPYKFYHGDWFCGKAVGLLVLHLSGLVLNAYVTHLHAEYSRQKDIYFAHRVAQAWELAQFIHHTSKKANVVLLCGDLNMHPKDLGCCLLKEWTGLRDAFVETEDFKGSEDGCTMVPKNCYVSQQDLGPFPFGVRIDYVLYKAVSGFHICCKTLKTTTGCDPHNGTPFSDHEALMATLCVKHSPPQEDPCSAHGSAERSALISALREARTELGRGIAQARWWAALFGYVMILGLSLLVLLCVLAAGEEAREVAIMLWTPSVGLVLGAGAVYLFHKQEAKSLCRAQAEIQHVLTRTTETQDLGSEPHPTHCRQQEADRAEEK

Target ORF information:

RefSeq Version	NM_031360.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingomyelin phosphodiesterase 2 (Smpd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031360.2

ORF Insert Sequence:

ATGAAGCACA ACTTTTCTCT GCGGCTGAGG GTTTTCAACC TCAACTGCTG GGACATCCCC 
TACCTAAGCA AGCATAGGGC CGACCGCATG AAGCGCTTGG GAGACTTTCT GAACTTGGAG 
AGCTTTGACC TGGCTCTGCT GGAGGAGGTG TGGAGTGAGC AGGACTTCCA GTACTTAAAG 
CAAAAGCTAT CGCTCACCTA CCCGGATGCA CACTACTTCA GAAGCGGAAT CATTGGCAGT 
GGCCTCTGTG TGTTCTCCAG ACACCCAATC CAGGAAATTG TCCAGCACGT CTATACTCTC 
AATGGTTACC CCTACAAGTT CTATCATGGA GACTGGTTCT GTGGGAAGGC TGTGGGGCTG 
CTGGTGCTCC ATCTAAGCGG ACTGGTGCTC AACGCCTACG TGACCCATCT GCACGCTGAG 
TACAGCCGGC AGAAGGATAT CTACTTCGCA CACCGTGTGG CCCAAGCTTG GGAACTGGCC 
CAGTTCATCC ACCACACATC CAAGAAAGCA AATGTGGTCC TGTTGTGCGG GGATCTCAAC 
ATGCACCCCA AAGACCTGGG CTGCTGCCTA CTGAAAGAGT GGACGGGGCT CCGTGATGCC 
TTTGTTGAGA CCGAGGACTT TAAGGGCTCA GAGGACGGCT GTACCATGGT ACCCAAGAAC 
TGCTACGTCA GCCAGCAGGA CCTGGGGCCG TTTCCCTTTG GCGTCCGTAT CGACTACGTG 
CTTTACAAGG CAGTCTCTGG CTTCCACATC TGCTGTAAGA CTCTGAAAAC CACTACGGGC 
TGTGACCCCC ACAATGGCAC CCCCTTCTCT GACCACGAGG CCCTCATGGC TACTTTGTGT 
GTGAAGCACA GCCCCCCTCA GGAAGACCCT TGCTCTGCCC ATGGATCAGC GGAAAGGTCA 
GCATTGATCA GCGCGCTAAG GGAGGCGAGG ACAGAGCTGG GGCGAGGCAT AGCTCAAGCT 
CGCTGGTGGG CTGCACTCTT TGGCTATGTG ATGATTTTGG GGCTGTCCCT CCTGGTGTTG 
CTGTGTGTCC TGGCTGCAGG AGAAGAGGCC AGGGAAGTGG CCATAATGCT CTGGACACCC 
AGTGTGGGTC TGGTGCTGGG GGCAGGCGCA GTCTACCTGT TCCACAAGCA GGAGGCCAAA 
AGCTTATGTC GGGCCCAGGC TGAGATACAA CATGTTCTGA CAAGGACAAC GGAGACCCAG 
GACCTAGGCT CAGAGCCTCA CCCAACCCAC TGCCGGCAGC AGGAGGCGGA CAGAGCTGAA 
GAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neutral sphingomyelinase inhibition alleviates apoptosis, but not ER stress, in liver ischemia-reperfusion injury.
Free radical research51(3)253-268(2017 Mar)
Tuzcu H,Unal B,K?rac E,Konuk E,Ozcan F,Elpek GO,Demir N,Aslan M

Involvement of neutral sphingomyelinase in the angiotensin II signaling pathway.
American journal of physiology. Renal physiology308(10)F1178-87(2015 May)
Bautista-P?rez R,del Valle-Mondrag?n L,Cano-Mart?nez A,P?rez-M?ndez O,Escalante B,Franco M

Trk receptors need neutral sphingomyelinase activity to promote cell viability.
FEBS letters588(1)167-74(2014 Jan)
Candalija A,Cub? R,Ortega A,Aguilera J,Gil C

Endocytosis and intracellular processing of BODIPY-sphingomyelin by murine CATH.a neurons.
Biochimica et biophysica acta1831(12)1665-78(2013 Dec)
Nusshold C,Uellen A,Bernhart E,Hammer A,Damm S,Wintersperger A,Reicher H,Hermetter A,Malle E,Sattler W

Endothelin-1 stimulates small artery VCAM-1 expression through p38MAPK-dependent neutral sphingomyelinase.
Journal of vascular research49(4)353-62(2012)
Ohanian J,Forman SP,Katzenberg G,Ohanian V

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Smpd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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