Ccn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccn1
Entrez Gene ID	83476
Full Name	cysteine-rich, angiogenic inducer, 61
General protein information	Preferred Names cysteine-rich, angiogenic inducer, 61 Names protein CYR61 CCN family member 1 IBP-10 IGF-binding protein 10 IGFBP-10 cysteine rich protein 61 insulin-like growth factor-binding protein 10
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q44
Summary	an extracellular heparin binding protein involved in supporting smooth muscle cell adhesion, promoting cell migration, and enhancing growth factor-stimulated mitogenesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031327.2	NP_112617.2	CCN family member 1 precursor

Pathways from BioSystems

Homologies

	CYR61	XP_001104992.2
Danio rerio (zebrafish)	zgc:85866	NP_001001826.1
Xenopus tropicalis (tropical clawed frog)	cyr61	NP_001098984.1
Canis lupus familiaris (dog)	CYR61	XP_537091.2
Rattus norvegicus (Norway rat)	Cyr61	NP_112617.2
Homo sapiens (human)	CYR61	NP_001545.2
Pan troglodytes (chimpanzee)	CYR61	NP_001092023.1
Mus musculus (house mouse)	Cyr61	NP_034646.1
Bos taurus (cattle)	CYR61	NP_001029512.1
Gallus gallus (chicken)	CYR61	NP_001026734.1

Gene Ontology
Bibliography

Related articles in PubMed

Interleukin 17 regulates SHP-2 and IL-17RA/STAT-3 dependent Cyr61, IL-23 and GM-CSF expression and RANKL mediated osteoclastogenesis by fibroblast-like synoviocytes in rheumatoid arthritis.
Ganesan R, Rasool M
Molecular immunology91134-144(2017 11)

Cysteine-rich 61 (Cyr61) upregulated in pulmonary arterial hypertension promotes the proliferation of pulmonary artery smooth muscle cells.
Gao L, Fan Y, Hao Y, Yuan P, Liu D, Jing Z, Zhang Z
International journal of medical sciences14(9)820-828(2017)

CCN1/CYR61 overexpression in hepatic stellate cells induces ER stress-related apoptosis.
Borkham-Kamphorst E, Steffen BT, Van de Leur E, Haas U, Tihaa L, Friedman SL, Weiskirchen R
Cellular signalling28(1)34-42(2016 Jan)

cAMP-induced actin cytoskeleton remodelling inhibits MKL1-dependent expression of the chemotactic and pro-proliferative factor, CCN1.
Duggirala A, Kimura TE, Sala-Newby GB, Johnson JL, Wu YJ, Newby AC, Bond M
Journal of molecular and cellular cardiology79157-68(2015 Feb)

Induction of the matricellular protein CCN1 through RhoA and MRTF-A contributes to ischemic cardioprotection.
Zhao X, Ding EY, Yu OM, Xiang SY, Tan-Sah VP, Yung BS, Hedgpeth J, Neubig RR, Lau LF, Brown JH, Miyamoto S
Journal of molecular and cellular cardiology75152-61(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings demonstrate that IL-17 regulates SHP-2 expression and IL-17RA/STAT-3 dependent production of Cyr61, IL-23, GM-CSF and RANKL in AA-FLS and may reveal a new insight into the pathogenesis of RA.
Title: Interleukin 17 regulates SHP-2 and IL-17RA/STAT-3 dependent Cyr61, IL-23 and GM-CSF expression and RANKL mediated osteoclastogenesis by fibroblast-like synoviocytes in rheumatoid arthritis.

the matricellular protein CCN1 gene transfer induces hepatic stellate cell apoptosis through endoplasmic reticulum stress and unfolded protein response
Title: CCN1/CYR61 overexpression in hepatic stellate cells induces ER stress-related apoptosis.

Cyclic AMP-induced actin-cytoskeleton remodelling regulates expression of CCN1 through MKL1.
Title: cAMP-induced actin cytoskeleton remodelling inhibits MKL1-dependent expression of the chemotactic and pro-proliferative factor, CCN1.

These findings implicate CCN1 as a mediator of cardioprotection induced by G-protein coupled receptor agonists that activate RhoA/MRTF-A signaling.
Title: Induction of the matricellular protein CCN1 through RhoA and MRTF-A contributes to ischemic cardioprotection.

CCN1, often co-expressed with FasL in a stressed heart, sensitizes cardiomyoblasts to FasL-induced apoptosis.
Title: CCN1 enables Fas ligand-induced apoptosis in cardiomyoblast H9c2 cells by disrupting caspase inhibitor XIAP.

The following Ccn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13382	NM_031327.2 Latest version!	Rattus norvegicus cellular communication network factor 1 (Ccn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccn1 ( NM_031327.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112617.2 Rattus norvegicus cellular communication network factor 1 (Ccn1), mRNA.

Price & Availability↑

CloneID	ORa13382
Clone ID Related Accession (Same CDS sequence)	NM_031327.2
Accession Version	NM_031327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	CCN family member 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF218568.1. On Jun 9, 2003 this sequence version replaced NM_031327.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF218568.1, BC081689.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTCCA GCACCATCAA GACGCTCGCT GTCGCCGTCA CCCTTCTCCA CTTGACCAGG 
CTGGCACTCT CCACCTGCCC TGCCGCCTGC CACTGCCCTC TGGAGGCGCC CAAGTGCGCC 
CCGGGAGTCG GCTTGGTCCG GGACGGCTGC GGCTGCTGTA AGGTCTGCGC GAAGCAACTC 
AACGAGGACT GCAGCAAAAC GCAGCCCTGC GACCACACCA AGGGGCTGGA ATGCAATTTC 
GGCGCCAGTT CCACCGCTCT GAAAGGGATC TGCAGAGCTC AGTCAGAAGG CAGACCCTGT 
GAATATAACT CCAGGATCTA CCAGAACGGG GAGAGCTTCC AACCCAACTG TAAACATCAG 
TGCACATGTA TTGACGGTGC TGTGGGCTGC ATTCCTCTGT GTCCCCAAGA ACTGTCTCTC 
CCCAATCTGG GCTGTCCCAA CCCCCGGCTG GTGAAAGTCA GCGGGCAGTG CTGTGAGGAA 
TGGGTCTGTG ATGAAGACAG CATTAAGGAC TCCCTGGACG ACCAGGACGA CCTCCTTGGA 
TTCGATGCCT CGGAGGTGGA GTTAACAAGA AACAATGAGT TAATCGCAAT TGGCAAAGGC 
AGCTCACTGA AGAGGCTTCC TGTCTTTGGC ACGGAACCTC GAGTCCTTTA CAACCCCCTG 
CATGCCCATG GCCAGAAATG CATCGTTCAG ACTACGTCCT GGTCCCAGTG CTCCAAGAGC 
TGCGGAACTG GCATCTCCAC ACGAGTTACC AATGACAACT CGGAGTGCCG CCTGGTGAAA 
GAGACCCGGA TCTGTGAAGT GCGTCCTTGT GGACAACCAG TGTACAGCAG CCTAAAAAAG 
GGCAAGAAAT GCAGCAAGAC CAAGAAATCC CCAGAACCAG TCCGATTTAC TTATGCAGGA 
TGCTCCAGTG TGAAGAAATA CCGGCCCAAA TACTGCGGCT CCTGCGTGGA CGGCCGGTGC 
TGCACACCTC TGCAGACCAG GACCGTGAAG ATGCGGTTCC GGTGCGAAGA TGGCGAGATG 
TTCTCCAAGA ACGTCATGAT GATTCAGTCC TGCAAGTGTA ACTACAACTG CCCGCATCCC 
AACGAGGCGT CGTTTCGCCT CTACAGTCTG TTCAACGATA TCCACAAGTT CAGGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112617.2
CDS	186..1325
Translation	MSSSTIKTLAVAVTLLHLTRLALSTCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVSGQCCEEWVCDEDSIKDSLDDQDDLLGFDASEVELTRNNELIAIGKGSSLKRLPVFGTEPRVLYNPLHAHGQKCIVQTTSWSQCSKSCGTGISTRVTNDNSECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCSSVKKYRPKYCGSCVDGRCCTPLQTRTVKMRFRCEDGEMFSKNVMMIQSCKCNYNCPHPNEASFRLYSLFNDIHKFRD

Target ORF information:

RefSeq Version	NM_031327.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cellular communication network factor 1 (Ccn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031327.2

ORF Insert Sequence:

ATGAGCTCCA GCACCATCAA GACGCTCGCT GTCGCCGTCA CCCTTCTCCA CTTGACCAGG 
CTGGCACTCT CCACCTGCCC TGCCGCCTGC CACTGCCCTC TGGAGGCGCC CAAGTGCGCC 
CCGGGAGTCG GCTTGGTCCG GGACGGCTGC GGCTGCTGTA AGGTCTGCGC GAAGCAACTC 
AACGAGGACT GCAGCAAAAC GCAGCCCTGC GACCACACCA AGGGGCTGGA ATGCAATTTC 
GGCGCCAGTT CCACCGCTCT GAAAGGGATC TGCAGAGCTC AGTCAGAAGG CAGACCCTGT 
GAATATAACT CCAGGATCTA CCAGAACGGG GAGAGCTTCC AACCCAACTG TAAACATCAG 
TGCACATGTA TTGACGGTGC TGTGGGCTGC ATTCCTCTGT GTCCCCAAGA ACTGTCTCTC 
CCCAATCTGG GCTGTCCCAA CCCCCGGCTG GTGAAAGTCA GCGGGCAGTG CTGTGAGGAA 
TGGGTCTGTG ATGAAGACAG CATTAAGGAC TCCCTGGACG ACCAGGACGA CCTCCTTGGA 
TTCGATGCCT CGGAGGTGGA GTTAACAAGA AACAATGAGT TAATCGCAAT TGGCAAAGGC 
AGCTCACTGA AGAGGCTTCC TGTCTTTGGC ACGGAACCTC GAGTCCTTTA CAACCCCCTG 
CATGCCCATG GCCAGAAATG CATCGTTCAG ACTACGTCCT GGTCCCAGTG CTCCAAGAGC 
TGCGGAACTG GCATCTCCAC ACGAGTTACC AATGACAACT CGGAGTGCCG CCTGGTGAAA 
GAGACCCGGA TCTGTGAAGT GCGTCCTTGT GGACAACCAG TGTACAGCAG CCTAAAAAAG 
GGCAAGAAAT GCAGCAAGAC CAAGAAATCC CCAGAACCAG TCCGATTTAC TTATGCAGGA 
TGCTCCAGTG TGAAGAAATA CCGGCCCAAA TACTGCGGCT CCTGCGTGGA CGGCCGGTGC 
TGCACACCTC TGCAGACCAG GACCGTGAAG ATGCGGTTCC GGTGCGAAGA TGGCGAGATG 
TTCTCCAAGA ACGTCATGAT GATTCAGTCC TGCAAGTGTA ACTACAACTG CCCGCATCCC 
AACGAGGCGT CGTTTCGCCT CTACAGTCTG TTCAACGATA TCCACAAGTT CAGGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interleukin 17 regulates SHP-2 and IL-17RA/STAT-3 dependent Cyr61, IL-23 and GM-CSF expression and RANKL mediated osteoclastogenesis by fibroblast-like synoviocytes in rheumatoid arthritis.
Molecular immunology91134-144(2017 11)
Ganesan R,Rasool M

Cysteine-rich 61 (Cyr61) upregulated in pulmonary arterial hypertension promotes the proliferation of pulmonary artery smooth muscle cells.
International journal of medical sciences14(9)820-828(2017)
Gao L,Fan Y,Hao Y,Yuan P,Liu D,Jing Z,Zhang Z

CCN1/CYR61 overexpression in hepatic stellate cells induces ER stress-related apoptosis.
Cellular signalling28(1)34-42(2016 Jan)
Borkham-Kamphorst E,Steffen BT,Van de Leur E,Haas U,Tihaa L,Friedman SL,Weiskirchen R

cAMP-induced actin cytoskeleton remodelling inhibits MKL1-dependent expression of the chemotactic and pro-proliferative factor, CCN1.
Journal of molecular and cellular cardiology79157-68(2015 Feb)
Duggirala A,Kimura TE,Sala-Newby GB,Johnson JL,Wu YJ,Newby AC,Bond M

Induction of the matricellular protein CCN1 through RhoA and MRTF-A contributes to ischemic cardioprotection.
Journal of molecular and cellular cardiology75152-61(2014 Oct)
Zhao X,Ding EY,Yu OM,Xiang SY,Tan-Sah VP,Yung BS,Hedgpeth J,Neubig RR,Lau LF,Brown JH,Miyamoto S

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Ccn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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