Cyp8b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp8b1
Entrez Gene ID	81924
Full Name	cytochrome P450 family 8 subfamily B member 1
General protein information	Preferred Names cytochrome P450 family 8 subfamily B member 1 Names 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase cytochrome P450, 8b1, sterol 12 alpha-hydrolase cytochrome P450, family 8, subfamily b, polypeptide 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	member of the cytochrome P450 enzyme superfamily; endoplasmic reticulum membrane protein; plays a role in sterol metabolism; human homolog is unique among cytochrome P450 genes in that it is intronless [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031241.1	NP_112520.1	7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC736962	XP_001140682.1
	LOC717247	XP_001108578.1
Gallus gallus (chicken)	CYP8B1	NP_001005571.1
Danio rerio (zebrafish)	cyp8b1	NP_001003736.1
Danio rerio (zebrafish)	cyp8b3	NP_001004587.1
Mus musculus (house mouse)	Cyp8b1	NP_034142.3
Canis lupus familiaris (dog)	LOC485619	XP_005634370.1
Rattus norvegicus (Norway rat)	Cyp8b1	NP_112520.1
Xenopus tropicalis (tropical clawed frog)	LOC100488168	XP_004915639.1
Homo sapiens (human)	CYP8B1	NP_004382.2
Danio rerio (zebrafish)	cyp8b2	NP_001103758.1
Xenopus tropicalis (tropical clawed frog)	LOC100488015	XP_002939350.1

Gene Ontology
Bibliography

Related articles in PubMed

Modulation of transport and metabolism of bile acids and bilirubin by chlorogenic acid against hepatotoxity and cholestasis in bile duct ligation rats: involvement of SIRT1-mediated deacetylation of FXR and PGC-1?.
Zhu L, Wang L, Cao F, Liu P, Bao H, Yan Y, Dong X, Wang D, Wang Z, Gong P
Journal of hepato-biliary-pancreatic sciences(2018 Jan)

Complex interaction between circadian rhythm and diet on bile acid homeostasis in male rats.
Eggink HM, Oosterman JE, de Goede P, de Vries EM, Foppen E, Koehorst M, Groen AK, Boelen A, Romijn JA, la Fleur SE, Soeters MR, Kalsbeek A
Chronobiology international34(10)1339-1353(2017)

Mechanisms of cholesterol and sterol regulatory element binding protein regulation of the sterol 12alpha-hydroxylase gene (CYP8B1).
Yang Y, Eggertsen G, G?fvels M, Andersson U, Einarsson C, Bj?rkhem I, Chiang JY
Biochemical and biophysical research communications320(4)1204-10(2004 Aug)

Structure, evolution, and liver-specific expression of sterol 12alpha-hydroxylase P450 (CYP8B).
Ishida H, Kuruta Y, Gotoh O, Yamashita C, Yoshida Y, Noshiro M
Journal of biochemistry126(1)19-25(1999 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cholesterol loading reduces SREBP-1 mRNA expression in addition to reducing functional SREBP-1 protein, and results in decreasing CYP8B1 gene transcription.
Title: Mechanisms of cholesterol and sterol regulatory element binding protein regulation of the sterol 12alpha-hydroxylase gene (CYP8B1).

The following Cyp8b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp8b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42213	NM_031241.1 Latest version!	Rattus norvegicus cytochrome P450 family 8 subfamily B member 1 (Cyp8b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp8b1 ( NM_031241.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112520.1 Rattus norvegicus cytochrome P450 family 8 subfamily B member 1 (Cyp8b1), mRNA.

Price & Availability↑

CloneID	ORa42213
Clone ID Related Accession (Same CDS sequence)	NM_031241.1
Accession Version	NM_031241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Rattus norvegicus(Norway rat)
Product	7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB009686.1. ##Evidence-Data-START## Transcript exon combination :: AB009686.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTGTGGG GTTCAGTGCT AGGGGCCCTG CTCATGGCTG TCGGTTGCCT CTGCCTCTCC 
TTGCTGCCCA GGCACCGCAG GCCCTGGGAG CCACCTCTGG ACAAGGGGTT TGTGCCCTGG 
CTGGGTCACA CCATGGCCTT CCGGAAGAAT ATGTTTGAAT TCCTGAAGGG AATGCGGGCC 
AAGCACGGGG ACGTGTTCAC ACTGCAGCTG GGAGGCCAGT ACTTCACCTT TGTCATGGAT 
CCCCTCTCCT TTGGCCCCAT CATTAAGAGC ACGCAGAAAG TGCTAGACTT CGTGACGTAC 
GCTCGGGAAC TGGTGTTCAA GGTGTTTGGG TACCAGTCCA TGGATGAGGA CCACCAGATG 
CTGCACGTAG CCAGTACCAA GCATCTGATG GGACAAGGCT TGGAGGACCT CAACCGGGCC 
ATGCTGGACA GCCTGTCCTT GGTGATGCTA GGACCCAAAG GCCGGAGTCT GGGTGCCAGG 
TCCTGGTGTG AGGACGGCCT CTTCCACTTC TGCTACAGCA TCTTGTTTAA GGCTGGCTTC 
CTGAGCTTGT TTGGCTGCAC CAAGGACAAG GAGCAGGACC TGGATGAGGC CGATGAGCTG 
TTCAGGAAGT TCCGCAGATT TGACCTACTT TTCCCCAGGT TTGTCTACTC CCTGCTGGGG 
CCCCTGGAGT GGGTAGAAGT GAGCCAGCTG CAGCGTCTCT TCCACCAGAG ACTCTCTGTG 
GAGCAAAACC TGGAGAAAGA CGGCATAAGT AACTGGTTGG GCTTCATGCT TCGGTTTCTG 
AGGGAGCGAG GGATGGCTTC ATCCATGCAG GACAAATTTA ACTTTATGAT GCTCTGGGCC 
TCCCAGGGGA ACACGGGGCC CACCTGTTTC TGGGCCCTCC TGTTCCTGCT GAAGCATCAG 
GACGCGATGA AGGCTGTGCG AGAGGAAGCC ACTCGGGTCC TGGGTGAGGC CAGGTTGGAA 
GCCGAGACAT CCTTCGCTTT CACTCTCAGT GCCCTGAAAT GCACTCCAGT GCTGGACAGC 
GTGATGGAAG AGACTCTTCG TCTCTGTGCC ACCCCCACCC TCCTCGGGGT GGTGCAGGAG 
GATTATGTCC TAAAGATGGC CAGTGGGCAA GAGTACCAGA TCCGCCGTGG AGATAAGGTG 
GCTCTCTTCC CCTATCTCTC AGTACACATG GACCCCGACA TCCATCCCGA GCCCACGACC 
TTCAAGTACA ATCGGTTCCT CAACCCTGAT GGCACCCGGA AAGTGGACTT CTACAAGTCA 
GGCAAGAAGA TCCACCACTA CAACATGCCC TGGGGGTCGG GTGTCTCCAT ATGTCCCGGC 
AGGTTCTTTG CCCCCAGTGA GATGAAGACC TTTGTCCTCC TTATGGTCAT GTACTTTGAC 
TTTGAACTGG TGGACCCTGA CATGCCTGTG CCCCCTATCG ACCCAAGGCG CTGGGGCTTC 
GGCACCTCCC AGCCCAGCCA TGAGGTACGC TTTCGCTACC GCCTGAAGCC CATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112520.1
CDS	34..1533
Translation	MLWGSVLGALLMAVGCLCLSLLPRHRRPWEPPLDKGFVPWLGHTMAFRKNMFEFLKGMRAKHGDVFTLQLGGQYFTFVMDPLSFGPIIKSTQKVLDFVTYARELVFKVFGYQSMDEDHQMLHVASTKHLMGQGLEDLNRAMLDSLSLVMLGPKGRSLGARSWCEDGLFHFCYSILFKAGFLSLFGCTKDKEQDLDEADELFRKFRRFDLLFPRFVYSLLGPLEWVEVSQLQRLFHQRLSVEQNLEKDGISNWLGFMLRFLRERGMASSMQDKFNFMMLWASQGNTGPTCFWALLFLLKHQDAMKAVREEATRVLGEARLEAETSFAFTLSALKCTPVLDSVMEETLRLCATPTLLGVVQEDYVLKMASGQEYQIRRGDKVALFPYLSVHMDPDIHPEPTTFKYNRFLNPDGTRKVDFYKSGKKIHHYNMPWGSGVSICPGRFFAPSEMKTFVLLMVMYFDFELVDPDMPVPPIDPRRWGFGTSQPSHEVRFRYRLKPMQ

Target ORF information:

RefSeq Version	NM_031241.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450 family 8 subfamily B member 1 (Cyp8b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031241.1

ORF Insert Sequence:

ATGCTGTGGG GTTCAGTGCT AGGGGCCCTG CTCATGGCTG TCGGTTGCCT CTGCCTCTCC 
TTGCTGCCCA GGCACCGCAG GCCCTGGGAG CCACCTCTGG ACAAGGGGTT TGTGCCCTGG 
CTGGGTCACA CCATGGCCTT CCGGAAGAAT ATGTTTGAAT TCCTGAAGGG AATGCGGGCC 
AAGCACGGGG ACGTGTTCAC ACTGCAGCTG GGAGGCCAGT ACTTCACCTT TGTCATGGAT 
CCCCTCTCCT TTGGCCCCAT CATTAAGAGC ACGCAGAAAG TGCTAGACTT CGTGACGTAC 
GCTCGGGAAC TGGTGTTCAA GGTGTTTGGG TACCAGTCCA TGGATGAGGA CCACCAGATG 
CTGCACGTAG CCAGTACCAA GCATCTGATG GGACAAGGCT TGGAGGACCT CAACCGGGCC 
ATGCTGGACA GCCTGTCCTT GGTGATGCTA GGACCCAAAG GCCGGAGTCT GGGTGCCAGG 
TCCTGGTGTG AGGACGGCCT CTTCCACTTC TGCTACAGCA TCTTGTTTAA GGCTGGCTTC 
CTGAGCTTGT TTGGCTGCAC CAAGGACAAG GAGCAGGACC TGGATGAGGC CGATGAGCTG 
TTCAGGAAGT TCCGCAGATT TGACCTACTT TTCCCCAGGT TTGTCTACTC CCTGCTGGGG 
CCCCTGGAGT GGGTAGAAGT GAGCCAGCTG CAGCGTCTCT TCCACCAGAG ACTCTCTGTG 
GAGCAAAACC TGGAGAAAGA CGGCATAAGT AACTGGTTGG GCTTCATGCT TCGGTTTCTG 
AGGGAGCGAG GGATGGCTTC ATCCATGCAG GACAAATTTA ACTTTATGAT GCTCTGGGCC 
TCCCAGGGGA ACACGGGGCC CACCTGTTTC TGGGCCCTCC TGTTCCTGCT GAAGCATCAG 
GACGCGATGA AGGCTGTGCG AGAGGAAGCC ACTCGGGTCC TGGGTGAGGC CAGGTTGGAA 
GCCGAGACAT CCTTCGCTTT CACTCTCAGT GCCCTGAAAT GCACTCCAGT GCTGGACAGC 
GTGATGGAAG AGACTCTTCG TCTCTGTGCC ACCCCCACCC TCCTCGGGGT GGTGCAGGAG 
GATTATGTCC TAAAGATGGC CAGTGGGCAA GAGTACCAGA TCCGCCGTGG AGATAAGGTG 
GCTCTCTTCC CCTATCTCTC AGTACACATG GACCCCGACA TCCATCCCGA GCCCACGACC 
TTCAAGTACA ATCGGTTCCT CAACCCTGAT GGCACCCGGA AAGTGGACTT CTACAAGTCA 
GGCAAGAAGA TCCACCACTA CAACATGCCC TGGGGGTCGG GTGTCTCCAT ATGTCCCGGC 
AGGTTCTTTG CCCCCAGTGA GATGAAGACC TTTGTCCTCC TTATGGTCAT GTACTTTGAC 
TTTGAACTGG TGGACCCTGA CATGCCTGTG CCCCCTATCG ACCCAAGGCG CTGGGGCTTC 
GGCACCTCCC AGCCCAGCCA TGAGGTACGC TTTCGCTACC GCCTGAAGCC CATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modulation of transport and metabolism of bile acids and bilirubin by chlorogenic acid against hepatotoxity and cholestasis in bile duct ligation rats: involvement of SIRT1-mediated deacetylation of FXR and PGC-1?.
Journal of hepato-biliary-pancreatic sciences(2018 Jan)
Zhu L,Wang L,Cao F,Liu P,Bao H,Yan Y,Dong X,Wang D,Wang Z,Gong P

Complex interaction between circadian rhythm and diet on bile acid homeostasis in male rats.
Chronobiology international34(10)1339-1353(2017)
Eggink HM,Oosterman JE,de Goede P,de Vries EM,Foppen E,Koehorst M,Groen AK,Boelen A,Romijn JA,la Fleur SE,Soeters MR,Kalsbeek A

Mechanisms of cholesterol and sterol regulatory element binding protein regulation of the sterol 12alpha-hydroxylase gene (CYP8B1).
Biochemical and biophysical research communications320(4)1204-10(2004 Aug)
Yang Y,Eggertsen G,G?fvels M,Andersson U,Einarsson C,Bj?rkhem I,Chiang JY

Structure, evolution, and liver-specific expression of sterol 12alpha-hydroxylase P450 (CYP8B).
Journal of biochemistry126(1)19-25(1999 Jul)
Ishida H,Kuruta Y,Gotoh O,Yamashita C,Yoshida Y,Noshiro M

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Cyp8b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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