Ube2d3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2d3
Entrez Gene ID	81920
Full Name	ubiquitin-conjugating enzyme E2D 3
General protein information	Preferred Names ubiquitin-conjugating enzyme E2D 3 Names ubiquitin-conjugating enzyme E2 D3 (E3-independent) E2 ubiquitin-conjugating enzyme D3 E2 ubiquitin-conjugating enzyme D3 E2(17)KB 3 PAPase phosphoarginine phosphatase ubiquitin carrier protein D3 ubiquitin-conjugating enzyme E2(17)KB 3 ubiquitin-conjugating enzyme E2-17 kDa 3 ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5) ubiquitin-protein ligase D3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q43
Summary	enzyme that has an important role in ubiquitin mediated proteolysis by conjugating ubiquitin to protein substrates [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006233333.2	XP_006233395.1	ubiquitin-conjugating enzyme E2 D3 isoform X1
XM_006233338.2	XP_006233400.1	ubiquitin-conjugating enzyme E2 D3 isoform X2
NM_031237.1	NP_112516.1	ubiquitin-conjugating enzyme E2 D3
XM_006233336.3	XP_006233398.1	ubiquitin-conjugating enzyme E2 D3 isoform X2
XM_006233335.2	XP_006233397.1	ubiquitin-conjugating enzyme E2 D3 isoform X1
XM_006233334.2	XP_006233396.1	ubiquitin-conjugating enzyme E2 D3 isoform X1
XM_006233337.2	XP_006233399.1	ubiquitin-conjugating enzyme E2 D3 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE2D3	NP_871616.1
Mus musculus (house mouse)	Ube2d3	NP_079632.1
Gallus gallus (chicken)	UBE2D3	NP_001026324.1
	UBE2D3	XP_001109666.1
Bos taurus (cattle)	UBE2D3	NP_001068603.1
Danio rerio (zebrafish)	ube2d3	NP_955865.1
Canis lupus familiaris (dog)	UBE2D3	XP_005642515.1
Rattus norvegicus (Norway rat)	Ube2d3	NP_112516.1
Oryza sativa (rice)	Os02g0120600	NP_001045711.2

Gene Ontology
Bibliography

Related articles in PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

Inorganic arsenic induces apoptosis through downregulation of Ube2d genes and p53 accumulation in rat proximal tubular cells.
Tokumoto M, Lee JY, Fujiwara Y, Uchiyama M, Satoh M
The Journal of toxicological sciences38(6)815-20(2013)

The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor.
Zhang L, Fairall L, Goult BT, Calkin AC, Hong C, Millard CJ, Tontonoz P, Schwabe JW
Genes & development25(12)1262-74(2011 Jun)

UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids.
Wenzel DM, Lissounov A, Brzovic PS, Klevit RE
Nature474(7349)105-8(2011 Jun)

Cadmium toxicity is caused by accumulation of p53 through the down-regulation of Ube2d family genes in vitro and in vivo.
Tokumoto M, Fujiwara Y, Shimada A, Hasegawa T, Seko Y, Nagase H, Satoh M
The Journal of toxicological sciences36(2)191-200(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ubiquitin-conjugating enzyme E2D 3 (UBE2D3), cAMP-dependent protein kinase C beta subunit (PKCbeta), excitatory amino acid carrier 1 (EAAC1), and ferritin heavy polypeptide 1 (Fth1), that were upregulated after a 48-h fast
Title: Identification of fasting-induced genes in the rat hypothalamus: relationship with neuroprotection.

The following Ube2d3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2d3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07784	NM_031237.1 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa07784	XM_006233336.3 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa07784	XM_006233338.2 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa07784	XM_006233337.2 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa25276	XM_006233333.2 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa25276	XM_006233335.2 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa25276	XM_006233334.2 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2d3 ( NM_031237.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112516.1 Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), mRNA.

Price & Availability↑

CloneID	ORa07784
Clone ID Related Accession (Same CDS sequence)	XM_006233338.2 , NM_031237.1 , XM_006233336.3 , XM_006233337.2
Accession Version	NM_031237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 D3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB006852.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB006852.1, CF110851.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAACAGAAT ATCTCGGGAA 
TGGACTCAGA AGTATGCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112516.1
CDS	274..717
Translation	MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM

Target ORF information:

RefSeq Version	NM_031237.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031237.1

ORF Insert Sequence:

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAACAGAAT ATCTCGGGAA 
TGGACTCAGA AGTATGCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2d3 ( XM_006233338.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233400.1 Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa07784
Clone ID Related Accession (Same CDS sequence)	XM_006233338.2 , NM_031237.1 , XM_006233336.3 , XM_006233337.2
Accession Version	XM_006233338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 D3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAACAGAAT ATCTCGGGAA 
TGGACTCAGA AGTATGCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233400.1
CDS	251..694
Translation	MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM

Target ORF information:

RefSeq Version	XM_006233338.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233338.2

ORF Insert Sequence:

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAACAGAAT ATCTCGGGAA 
TGGACTCAGA AGTATGCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2d3 ( XM_006233336.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233398.1 Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa07784
Clone ID Related Accession (Same CDS sequence)	XM_006233338.2 , NM_031237.1 , XM_006233336.3 , XM_006233337.2
Accession Version	XM_006233336.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 D3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233336.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAACAGAAT ATCTCGGGAA 
TGGACTCAGA AGTATGCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233398.1
CDS	268..711
Translation	MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM

Target ORF information:

RefSeq Version	XM_006233336.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233336.3

ORF Insert Sequence:

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAACAGAAT ATCTCGGGAA 
TGGACTCAGA AGTATGCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2d3 ( XM_006233337.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233399.1 Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa07784
Clone ID Related Accession (Same CDS sequence)	XM_006233338.2 , NM_031237.1 , XM_006233336.3 , XM_006233337.2
Accession Version	XM_006233337.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 D3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233337.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAACAGAAT ATCTCGGGAA 
TGGACTCAGA AGTATGCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233399.1
CDS	413..856
Translation	MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM

Target ORF information:

RefSeq Version	XM_006233337.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233337.2

ORF Insert Sequence:

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAACAGAAT ATCTCGGGAA 
TGGACTCAGA AGTATGCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2d3 ( XM_006233333.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233395.1 Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25276
Clone ID Related Accession (Same CDS sequence)	XM_006233333.2 , XM_006233335.2 , XM_006233334.2
Accession Version	XM_006233333.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 D3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAATAGGTT AGCAAGAGAG 
TGGACAGAGA AATACGCTAT GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233395.1
CDS	270..716
Translation	MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRLAREWTEKYAML

Target ORF information:

RefSeq Version	XM_006233333.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233333.2

ORF Insert Sequence:

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAATAGGTT AGCAAGAGAG 
TGGACAGAGA AATACGCTAT GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2d3 ( XM_006233335.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233397.1 Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa25276
Clone ID Related Accession (Same CDS sequence)	XM_006233333.2 , XM_006233335.2 , XM_006233334.2
Accession Version	XM_006233335.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 D3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233335.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAATAGGTT AGCAAGAGAG 
TGGACAGAGA AATACGCTAT GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233397.1
CDS	238..684
Translation	MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRLAREWTEKYAML

Target ORF information:

RefSeq Version	XM_006233335.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233335.2

ORF Insert Sequence:

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAATAGGTT AGCAAGAGAG 
TGGACAGAGA AATACGCTAT GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2d3 ( XM_006233334.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233396.1 Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa25276
Clone ID Related Accession (Same CDS sequence)	XM_006233333.2 , XM_006233335.2 , XM_006233334.2
Accession Version	XM_006233334.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 D3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233334.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAATAGGTT AGCAAGAGAG 
TGGACAGAGA AATACGCTAT GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233396.1
CDS	413..859
Translation	MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRLAREWTEKYAML

Target ORF information:

RefSeq Version	XM_006233334.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2D 3 (Ube2d3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233334.2

ORF Insert Sequence:

ATGGCGCTGA AACGGATTAA TAAGGAACTT AGTGATTTGG CCCGTGACCC TCCAGCACAA 
TGTTCTGCAG GTCCAGTTGG AGATGACATG TTTCATTGGC AAGCCACAAT TATGGGACCT 
AATGACAGCC CATATCAAGG TGGTGTATTC TTTTTGACAA TTCATTTTCC TACAGACTAC 
CCCTTCAAAC CACCTAAGGT TGCATTTACA ACAAGAATTT ATCATCCAAA TATTAACAGT 
AATGGCAGCA TTTGTCTTGA TATTCTAAGA TCACAGTGGT CTCCTGCTTT AACTATTTCT 
AAAGTTCTTT TATCCATTTG TTCACTGCTA TGTGATCCAA ACCCAGATGA CCCCCTAGTG 
CCAGAGATTG CACGGATCTA TAAAACAGAC AGAGACAAGT ACAATAGGTT AGCAAGAGAG 
TGGACAGAGA AATACGCTAT GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

Inorganic arsenic induces apoptosis through downregulation of Ube2d genes and p53 accumulation in rat proximal tubular cells.
The Journal of toxicological sciences38(6)815-20(2013)
Tokumoto M,Lee JY,Fujiwara Y,Uchiyama M,Satoh M

The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor.
Genes & development25(12)1262-74(2011 Jun)
Zhang L,Fairall L,Goult BT,Calkin AC,Hong C,Millard CJ,Tontonoz P,Schwabe JW

UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids.
Nature474(7349)105-8(2011 Jun)
Wenzel DM,Lissounov A,Brzovic PS,Klevit RE

Cadmium toxicity is caused by accumulation of p53 through the down-regulation of Ube2d family genes in vitro and in vivo.
The Journal of toxicological sciences36(2)191-200(2011 Apr)
Tokumoto M,Fujiwara Y,Shimada A,Hasegawa T,Seko Y,Nagase H,Satoh M

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Ube2d3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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