Slc20a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc20a1
Entrez Gene ID	81826
Full Name	solute carrier family 20 member 1
Synonyms	PiT-1
General protein information	Preferred Names solute carrier family 20 member 1 Names sodium-dependent phosphate transporter 1 RPHO-1 phosphate transporter 1 solute carrier family 20 (phosphate transporter), member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q36
Summary	a Na+-dependent Pi cotransporter; may be involved in Pi and vitamin D function in the parathyroid [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006234992.3	XP_006235054.1	sodium-dependent phosphate transporter 1 isoform X2
XM_006234991.3	XP_006235053.1	sodium-dependent phosphate transporter 1 isoform X1
NM_031148.1	NP_112410.1	sodium-dependent phosphate transporter 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC20A1	NP_005406.3
	SLC20A1	XP_001089612.1
Canis lupus familiaris (dog)	SLC20A1	XP_540181.2
Bos taurus (cattle)	SLC20A1	NP_001178114.1
Caenorhabditis elegans (roundworm)	C48A7.2	NP_501180.1
Magnaporthe oryzae (rice blast fungus)	MGG_07966	XP_003713212.1
Pan troglodytes (chimpanzee)	SLC20A1	XP_515694.2
Mus musculus (house mouse)	Slc20a1	NP_056562.1
Danio rerio (zebrafish)	slc20a1b	NP_997753.1
Rattus norvegicus (Norway rat)	Slc20a1	NP_112410.1
Gallus gallus (chicken)	SLC20A1	XP_003642605.2
Xenopus tropicalis (tropical clawed frog)	slc20a1	NP_001025568.1

Gene Ontology
Bibliography

Related articles in PubMed

Magnesium prevents phosphate-induced vascular calcification via TRPM7 and Pit-1 in an aortic tissue culture model.
Sonou T, Ohya M, Yashiro M, Masumoto A, Nakashima Y, Ito T, Mima T, Negi S, Kimura-Suda H, Shigematsu T
Hypertension research : official journal of the Japanese Society of Hypertension40(6)562-567(2017 Jun)

Activating transcription factor-4 promotes mineralization in vascular smooth muscle cells.
Masuda M, Miyazaki-Anzai S, Keenan AL, Shiozaki Y, Okamura K, Chick WS, Williams K, Zhao X, Rahman SM, Tintut Y, Adams CM, Miyazaki M
JCI insight1(18)e88646(2016 Nov)

Effect of hyperphosphatemia on gene expression of the Na-Pi cotransporter in rats.
Zhang C, Shi LM, Li Y, Zhu QG, Jin CL, Wang HP, Zhou L, Hu XP, Zhang L, Wang H, Yuan GJ, Xu L, Zhao YK, Chou Y
Genetics and molecular research : GMR14(4)19404-10(2016 Jan)

Apelin: A novel inhibitor of vascular calcification in chronic kidney disease.
Han X, Wang LY, Diao ZL, Liu WH
Atherosclerosis2441-8(2016 Jan)

Phosphate handling: new genes, new molecules.
Pri? D, Torres PU, Friedlander G
Hormone research in paediatrics76 Suppl 171-5(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NaPi-III mRNA expression is affected by hyperphosphatemia.
Title: Effect of hyperphosphatemia on gene expression of the Na-Pi cotransporter in rats.

Apelin ameliorates VC by suppressing osteoblastic differentiation of VSMCs through downregulation of Pit-1.
Title: Apelin: A novel inhibitor of vascular calcification in chronic kidney disease.

Incisor enamel formation is impaired in transgenic rats overexpressing the type III NaPi transporter Slc20a1.
Title: Incisor enamel formation is impaired in transgenic rats overexpressing the type III NaPi transporter Slc20a1.

Dexamethasone and cyclic AMP regulate sodium phosphate cotransporter (NaPi-IIb and Pit-1) mRNA and phosphate uptake in rat alveolar type II epithelial cells.
Title: Dexamethasone and cyclic AMP regulate sodium phosphate cotransporter (NaPi-IIb and Pit-1) mRNA and phosphate uptake in rat alveolar type II epithelial cells.

phosphate uptake through the type III sodium-dependent phosphate cotransporter, Pit-1, leads to induction of apoptosis and subsequent calcification of VSMC.
Title: Mechanism of phosphate-induced calcification in rat aortic tissue culture: possible involvement of Pit-1 and apoptosis.

The following Slc20a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc20a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07753	NM_031148.1 Latest version!	Rattus norvegicus solute carrier family 20 member 1 (Slc20a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa26370	XM_006234992.3 Latest version!	Rattus norvegicus solute carrier family 20 member 1 (Slc20a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa26369	XM_006234991.3 Latest version!	Rattus norvegicus solute carrier family 20 member 1 (Slc20a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc20a1 ( NM_031148.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112410.1 Rattus norvegicus solute carrier family 20 member 1 (Slc20a1), mRNA.

Price & Availability↑

CloneID	ORa07753
Clone ID Related Accession (Same CDS sequence)	NM_031148.1
Accession Version	NM_031148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	sodium-dependent phosphate transporter 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB000489.1. Sequence Note: This sequence has been modified as follows: removed 40 bp suspected to be vector contamination from the 5' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB000489.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCTA CCCTGGCCCC GATTACTAGT ACTCTAGCTG CTGTTACTGC TTCTGCTCCA 
CCGAAGTATG ACAATCTGTG GATGCTCATC CTGGGCTTCA TCATTGCATT TGTCTTGGCA 
TTCTCCGTGG GAGCCAATGA TGTAGCAAAT TCATTTGGTA CAGCTGTAGG CTCAGGTGTA 
GTGACCCTGA AGCAAGCCTG CATCCTAGCT AGCATCTTCG AAACTGTGGG CTCCGCCTTG 
CTGGGGGCCA AAGTGAGTGA AACCATCCGG AAGGGCTTGA TAGATGTGGA GAAGTACAAC 
GCAACTCAAG ATCTGCTCAT GGCTGGCTCT GTCAGTGCTA TGTTTGGTTC TGCTGTGTGG 
CAACTAGTGG CTTCGTTTTT GAAGCTTCCT ATTTCTGGGA CCCATTGTAT TGTCGGTGCA 
ACCATTGGTT TCTCCCTTGT GGCAAAGGGA CAAGAGGGTA TCAAATGGTC CGAACTGATA 
AAAATTGTGA TGTCTTGGTT CGTCTCTCCA CTGCTTTCTG GTATTATGTC TGGAATTTTA 
TTCTTCCTTG TTCGTGCATT CATCCTCCGT AAGGCAGATC CAGTTCCTAA TGGCTTACGA 
GCTTTACCCA TTTTTTATGC CTGCACAATT GGAATCAACC TCTTTTCCAT TATGTATACT 
GGAGCACCAT TGCTGGGCTT TGACAAACTT CCTCTGTGGG GTACCATCCT CATCTCGGTG 
GGATGTGCAG TTTTCTGTGC CCTTATCGTC TGGTTCTTCG TATGTCCCAG GATGAAGAGG 
AAAATTGAAC GAGAAGTAAA GTCTAGTCCC TCTGAAAGTC CCTTAATGGA AAAGAAGAAC 
AACTTAAAAG ACCATGAAGA AACAAAGATG GCTCCTGGCG ATGTTGAGAA TAGGAATCCT 
GTGTCTGAGG TAGTATGTGC CACTGGCCCA CTACGGGCTG TGGTGGAGGA GAGAACGGTG 
TCATTTAAAC TCGGTGACCT GGAGGAAGCT CCGGAGCGAG AGCGGCTTCC CATGGACCTG 
AAGGAGGAGA CCAACATAGA TGGTACTATC AATGGTGCAG TGCAGTTGCC TAATGGGAAC 
CTTGTTCAGT TCAGTCAAAC CGTCAGCAAC CAGATCAACT CCAGTGGCCA CTATCAGTAT 
CACACCGTGC ACAAGGATTC TGGCTTGTAC AAAGAGCTGC TCCATAAGCT ACATCTGGCC 
AAGGTGGGAG ACTGCATGGG AGACTCTGGG GACAAGCCCT TGAGACGCAA CAACAGCTAC 
ACATCCTACA CCATGGCAAT ATGTGGCATG CCCCTGGATT CCTTCCGTGC CAAAGAAGGG 
GAACAAAAGG GAGACGAAAT GGAAACGCTG ACTTGGCCTA ATGCAGATAC CAAGAAGCGG 
ATTCGGATGG ACAGTTACAC CAGTTACTGC AATGCTGTGT CTGACCTTCA CTCAGAGTCT 
GAGATGGACA TGAGTGTTAA GGCAGAGATG GGTCTGGGTG ACAGAAAAGG AAGCAGTGGC 
TCTCTTGAAG AATGGTATGA TCAGGATAAG CCTGAAGTGT CCCTTCTCTT CCAGTTTCTG 
CAGATCCTTA CAGCCTGCTT TGGGTCCTTT GCCCATGGTG GCAATGACGT CAGCAATGCC 
ATCGGCCCTC TGGTTGCTTT GTATCTTGTT TATGAAACGA GAGATGTCAC TACAAAAGAG 
GCAACACCCA TATGGCTTCT GCTTTATGGT GGTGTTGGCA TTTGCATGGG GCTATGGGTT 
TGGGGAAGAA GAGTTATCCA GACTATGGGG AAGGACCTGA CACCAATCAC ACCCTCCAGT 
GGTTTCAGTA TTGAACTGGC ATCTGCCTTC ACTGTGGTCG TCGCATCAAA CATTGGCCTT 
CCCATCAGCA CAACACATTG TAAGGTGGGC TCTGTGGTGT CTGTTGGCTG GCTCCGATCC 
AAGAAGGCTG TTGACTGGCG ACTGTTTCGA AACATTTTTA TGGCCTGGTT TGTCACCGTC 
CCTATTTCTG GGGTTATCAG TGCTGCTATC ATGGCAGTGT TCAAGCACAT CATTCTGCCG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112410.1
CDS	52..2097
Translation	MASTLAPITSTLAAVTASAPPKYDNLWMLILGFIIAFVLAFSVGANDVANSFGTAVGSGVVTLKQACILASIFETVGSALLGAKVSETIRKGLIDVEKYNATQDLLMAGSVSAMFGSAVWQLVASFLKLPISGTHCIVGATIGFSLVAKGQEGIKWSELIKIVMSWFVSPLLSGIMSGILFFLVRAFILRKADPVPNGLRALPIFYACTIGINLFSIMYTGAPLLGFDKLPLWGTILISVGCAVFCALIVWFFVCPRMKRKIEREVKSSPSESPLMEKKNNLKDHEETKMAPGDVENRNPVSEVVCATGPLRAVVEERTVSFKLGDLEEAPERERLPMDLKEETNIDGTINGAVQLPNGNLVQFSQTVSNQINSSGHYQYHTVHKDSGLYKELLHKLHLAKVGDCMGDSGDKPLRRNNSYTSYTMAICGMPLDSFRAKEGEQKGDEMETLTWPNADTKKRIRMDSYTSYCNAVSDLHSESEMDMSVKAEMGLGDRKGSSGSLEEWYDQDKPEVSLLFQFLQILTACFGSFAHGGNDVSNAIGPLVALYLVYETRDVTTKEATPIWLLLYGGVGICMGLWVWGRRVIQTMGKDLTPITPSSGFSIELASAFTVVVASNIGLPISTTHCKVGSVVSVGWLRSKKAVDWRLFRNIFMAWFVTVPISGVISAAIMAVFKHIILPV

Target ORF information:

RefSeq Version	NM_031148.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 20 member 1 (Slc20a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031148.1

ORF Insert Sequence:

ATGGCATCTA CCCTGGCCCC GATTACTAGT ACTCTAGCTG CTGTTACTGC TTCTGCTCCA 
CCGAAGTATG ACAATCTGTG GATGCTCATC CTGGGCTTCA TCATTGCATT TGTCTTGGCA 
TTCTCCGTGG GAGCCAATGA TGTAGCAAAT TCATTTGGTA CAGCTGTAGG CTCAGGTGTA 
GTGACCCTGA AGCAAGCCTG CATCCTAGCT AGCATCTTCG AAACTGTGGG CTCCGCCTTG 
CTGGGGGCCA AAGTGAGTGA AACCATCCGG AAGGGCTTGA TAGATGTGGA GAAGTACAAC 
GCAACTCAAG ATCTGCTCAT GGCTGGCTCT GTCAGTGCTA TGTTTGGTTC TGCTGTGTGG 
CAACTAGTGG CTTCGTTTTT GAAGCTTCCT ATTTCTGGGA CCCATTGTAT TGTCGGTGCA 
ACCATTGGTT TCTCCCTTGT GGCAAAGGGA CAAGAGGGTA TCAAATGGTC CGAACTGATA 
AAAATTGTGA TGTCTTGGTT CGTCTCTCCA CTGCTTTCTG GTATTATGTC TGGAATTTTA 
TTCTTCCTTG TTCGTGCATT CATCCTCCGT AAGGCAGATC CAGTTCCTAA TGGCTTACGA 
GCTTTACCCA TTTTTTATGC CTGCACAATT GGAATCAACC TCTTTTCCAT TATGTATACT 
GGAGCACCAT TGCTGGGCTT TGACAAACTT CCTCTGTGGG GTACCATCCT CATCTCGGTG 
GGATGTGCAG TTTTCTGTGC CCTTATCGTC TGGTTCTTCG TATGTCCCAG GATGAAGAGG 
AAAATTGAAC GAGAAGTAAA GTCTAGTCCC TCTGAAAGTC CCTTAATGGA AAAGAAGAAC 
AACTTAAAAG ACCATGAAGA AACAAAGATG GCTCCTGGCG ATGTTGAGAA TAGGAATCCT 
GTGTCTGAGG TAGTATGTGC CACTGGCCCA CTACGGGCTG TGGTGGAGGA GAGAACGGTG 
TCATTTAAAC TCGGTGACCT GGAGGAAGCT CCGGAGCGAG AGCGGCTTCC CATGGACCTG 
AAGGAGGAGA CCAACATAGA TGGTACTATC AATGGTGCAG TGCAGTTGCC TAATGGGAAC 
CTTGTTCAGT TCAGTCAAAC CGTCAGCAAC CAGATCAACT CCAGTGGCCA CTATCAGTAT 
CACACCGTGC ACAAGGATTC TGGCTTGTAC AAAGAGCTGC TCCATAAGCT ACATCTGGCC 
AAGGTGGGAG ACTGCATGGG AGACTCTGGG GACAAGCCCT TGAGACGCAA CAACAGCTAC 
ACATCCTACA CCATGGCAAT ATGTGGCATG CCCCTGGATT CCTTCCGTGC CAAAGAAGGG 
GAACAAAAGG GAGACGAAAT GGAAACGCTG ACTTGGCCTA ATGCAGATAC CAAGAAGCGG 
ATTCGGATGG ACAGTTACAC CAGTTACTGC AATGCTGTGT CTGACCTTCA CTCAGAGTCT 
GAGATGGACA TGAGTGTTAA GGCAGAGATG GGTCTGGGTG ACAGAAAAGG AAGCAGTGGC 
TCTCTTGAAG AATGGTATGA TCAGGATAAG CCTGAAGTGT CCCTTCTCTT CCAGTTTCTG 
CAGATCCTTA CAGCCTGCTT TGGGTCCTTT GCCCATGGTG GCAATGACGT CAGCAATGCC 
ATCGGCCCTC TGGTTGCTTT GTATCTTGTT TATGAAACGA GAGATGTCAC TACAAAAGAG 
GCAACACCCA TATGGCTTCT GCTTTATGGT GGTGTTGGCA TTTGCATGGG GCTATGGGTT 
TGGGGAAGAA GAGTTATCCA GACTATGGGG AAGGACCTGA CACCAATCAC ACCCTCCAGT 
GGTTTCAGTA TTGAACTGGC ATCTGCCTTC ACTGTGGTCG TCGCATCAAA CATTGGCCTT 
CCCATCAGCA CAACACATTG TAAGGTGGGC TCTGTGGTGT CTGTTGGCTG GCTCCGATCC 
AAGAAGGCTG TTGACTGGCG ACTGTTTCGA AACATTTTTA TGGCCTGGTT TGTCACCGTC 
CCTATTTCTG GGGTTATCAG TGCTGCTATC ATGGCAGTGT TCAAGCACAT CATTCTGCCG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc20a1 ( XM_006234992.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235054.1 Rattus norvegicus solute carrier family 20 member 1 (Slc20a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa26370
Clone ID Related Accession (Same CDS sequence)	XM_006234992.3
Accession Version	XM_006234992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium-dependent phosphate transporter 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTA CCCTGGCCCC GATTACTAGT ACTCTAGCTG CTGTTACTGC TTCTGCTCCA 
CCGAAGTATG ACAATCTGTG GATGCTCATC CTGGGCTTCA TCATTGCATT TGTCTTGGCA 
TTCTCCGTGG GAGCCAATGA TGTAGCAAAT TCATTTGGTA CAGCTGTAGG CTCAGGTGTA 
GTGACCCTGA AGCAAGCCTG CATCCTAGCT AGCATCTTCG AAACTGTGGG CTCCGCCTTG 
CTGGGGGCCA AAGTGAGTGA AACCATCCGG AAGGGCTTGA TAGATGTGGA GAAGTACAAC 
GCAACTCAAG ATCTGCTCAT GGCTGGCTCT GTCAGTGCTA TGTTTGGTTC TGCTGTGTGG 
CAACTAGTGG CTTCGTTTTT GAAGCTTCCT ATTTCTGGGA CCCATTGTAT TGTCGGTGCA 
ACCATTGGTT TCTCCCTTGT GGCAAAGGGA CAAGAGGGTA TCAAATGGTC CGAACTGATA 
AAAATTGTGA TGTCTTGGTT CGTCTCTCCA CTGCTTTCTG GTATTATGTC TGGAATTTTA 
TTCTTCCTTG TTCGTGCATT CATCCTCCGT AAGGCAGATC CAGTTCCTAA TGGCTTACGA 
GCTTTACCCA TTTTTTATGC CTGCACAATT GGAATCAACC TCTTTTCCAT TATGTATACT 
GGAGCACCAT GGCTGAAATC TCCTAGAAGG TGGCTGGCCT GCACCCATTT CAGAAGGCTG 
CAGGCTAGTG TATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235054.1
CDS	471..1208
Translation	MASTLAPITSTLAAVTASAPPKYDNLWMLILGFIIAFVLAFSVGANDVANSFGTAVGSGVVTLKQACILASIFETVGSALLGAKVSETIRKGLIDVEKYNATQDLLMAGSVSAMFGSAVWQLVASFLKLPISGTHCIVGATIGFSLVAKGQEGIKWSELIKIVMSWFVSPLLSGIMSGILFFLVRAFILRKADPVPNGLRALPIFYACTIGINLFSIMYTGAPWLKSPRRWLACTHFRRLQASVC

Target ORF information:

RefSeq Version	XM_006234992.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 20 member 1 (Slc20a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234992.3

ORF Insert Sequence:

ATGGCATCTA CCCTGGCCCC GATTACTAGT ACTCTAGCTG CTGTTACTGC TTCTGCTCCA 
CCGAAGTATG ACAATCTGTG GATGCTCATC CTGGGCTTCA TCATTGCATT TGTCTTGGCA 
TTCTCCGTGG GAGCCAATGA TGTAGCAAAT TCATTTGGTA CAGCTGTAGG CTCAGGTGTA 
GTGACCCTGA AGCAAGCCTG CATCCTAGCT AGCATCTTCG AAACTGTGGG CTCCGCCTTG 
CTGGGGGCCA AAGTGAGTGA AACCATCCGG AAGGGCTTGA TAGATGTGGA GAAGTACAAC 
GCAACTCAAG ATCTGCTCAT GGCTGGCTCT GTCAGTGCTA TGTTTGGTTC TGCTGTGTGG 
CAACTAGTGG CTTCGTTTTT GAAGCTTCCT ATTTCTGGGA CCCATTGTAT TGTCGGTGCA 
ACCATTGGTT TCTCCCTTGT GGCAAAGGGA CAAGAGGGTA TCAAATGGTC CGAACTGATA 
AAAATTGTGA TGTCTTGGTT CGTCTCTCCA CTGCTTTCTG GTATTATGTC TGGAATTTTA 
TTCTTCCTTG TTCGTGCATT CATCCTCCGT AAGGCAGATC CAGTTCCTAA TGGCTTACGA 
GCTTTACCCA TTTTTTATGC CTGCACAATT GGAATCAACC TCTTTTCCAT TATGTATACT 
GGAGCACCAT GGCTGAAATC TCCTAGAAGG TGGCTGGCCT GCACCCATTT CAGAAGGCTG 
CAGGCTAGTG TATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc20a1 ( XM_006234991.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235053.1 Rattus norvegicus solute carrier family 20 member 1 (Slc20a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26369
Clone ID Related Accession (Same CDS sequence)	XM_006234991.3
Accession Version	XM_006234991.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium-dependent phosphate transporter 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234991.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTA CCCTGGCCCC GATTACTAGT ACTCTAGCTG CTGTTACTGC TTCTGCTCCA 
CCGAAGTATG ACAATCTGTG GATGCTCATC CTGGGCTTCA TCATTGCATT TGTCTTGGCA 
TTCTCCGTGG GAGCCAATGA TGTAGCAAAT TCATTTGGTA CAGCTGTAGG CTCAGGTGTA 
GTGACCCTGA AGCAAGCCTG CATCCTAGCT AGCATCTTCG AAACTGTGGG CTCCGCCTTG 
CTGGGGGCCA AAGTGAGTGA AACCATCCGG AAGGGCTTGA TAGATGTGGA GAAGTACAAC 
GCAACTCAAG ATCTGCTCAT GGCTGGCTCT GTCAGTGCTA TGTTTGGTTC TGCTGTGTGG 
CAACTAGTGG CTTCGTTTTT GAAGCTTCCT ATTTCTGGGA CCCATTGTAT TGTCGGTGCA 
ACCATTGGTT TCTCCCTTGT GGCAAAGGGA CAAGAGGGTA TCAAATGGTC CGAACTGATA 
AAAATTGTGA TGTCTTGGTT CGTCTCTCCA CTGCTTTCTG GTATTATGTC TGGAATTTTA 
TTCTTCCTTG TTCGTGCATT CATCCTCCGT AAGGCAGATC CAGTTCCTAA TGGCTTACGA 
GCTTTACCCA TTTTTTATGC CTGCACAATT GGAATCAACC TCTTTTCCAT TATGTATACT 
GGAGCACCAT TGCTGGGCTT TGACAAACTT CCTCTGTGGG GTACCATCCT CATCTCGGTG 
GGATGTGCAG TTTTCTGTGC CCTTATCGTC TGGTTCTTTG TATGTCCCAG GATGAAGAGG 
AAAATTGAAC GAGAAGTAAA GTCTAGTCCC TCTGAAAGTC CCTTAATGGA AAAGAAGAAC 
AACTTAAAAG ACCATGAAGA AACAAAGATG GCTCCTGGCG ATGTTGAGAA TAGGAATCCT 
GTGTCTGAGG TAGTATGTGC CACTGGCCCA CTACGGGCTG TGGTGGAGGA GAGAACGGTG 
TCATTTAAAC TCGGTGACCT GGAGGAAGCT CCGGAGCGAG AGCGGCTTCC CATGGACCTG 
AAGGAGGAGA CCAACATAGA TGGTACTATC AATGGTGCAG TGCAGTTGCC TAATGGGAAC 
CTTGTTCAGT TCAGTCAAAC CGTCAGCAAC CAGATCAACT CCAGTGGCCA CTATCAGTAT 
CACACCGTGC ACAAGGATTC TGGCTTGTAC AAAGAGCTGC TCCATAAGCT ACATCTGGCC 
AAGGTGGGAG ACTGCATGGG AGACTCTGGG GACAAGCCCT TGAGACGCAA CAACAGCTAC 
ACATCCTACA CCATGGCAAT ATGTGGCATG CCCCTGGATT CCTTCCGTGC CAAAGAAGGG 
GAACAAAAGG GAGACGAAAT GGAAACGCTG ACTTGGCCTA ATGCAGATAC CAAGAAGCGG 
ATTCGGATGG ACAGTTACAC CAGTTACTGC AATGCTGTGT CTGACCTTCA CTCAGAGTCT 
GAGATGGACA TGAGTGTTAA GGCAGAGATG GGTCTGGGTG ACAGAAAAGG AAGCAGTGGC 
TCTCTTGAAG AATGGTATGA TCAGGATAAG CCTGAAGTGT CCCTTCTCTT CCAGTTTCTG 
CAGATCCTTA CAGCCTGCTT TGGGTCCTTT GCCCATGGTG GCAATGACGT CAGCAATGCC 
ATCGGCCCTC TGGTTGCTTT GTATCTTGTT TATGAAACGA GAGATGTCAC TACAAAAGAG 
GCAACACCCA TATGGCTTCT GCTTTATGGT GGTGTTGGCA TTTGCATGGG GCTATGGGTT 
TGGGGAAGAA GAGTTATCCA GACTATGGGG AAGGACCTGA CACCAATCAC ACCCTCCAGT 
GGTTTCAGTA TTGAACTGGC ATCTGCCTTC ACTGTGGTCG TCGCATCAAA CATTGGCCTT 
CCCATCAGCA CAACACATTG TAAGGTGGGC TCTGTGGTGT CTGTTGGCTG GCTCCGATCC 
AAGAAGGCTG TTGACTGGCG ACTGTTTCGA AACATTTTTA TGGCCTGGTT TGTCACCGTC 
CCTATTTCTG GGGTTATCAG TGCTGCTATC ATGGCAGTGT TCAAGCACAT CATTCTGCCG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235053.1
CDS	470..2515
Translation	MASTLAPITSTLAAVTASAPPKYDNLWMLILGFIIAFVLAFSVGANDVANSFGTAVGSGVVTLKQACILASIFETVGSALLGAKVSETIRKGLIDVEKYNATQDLLMAGSVSAMFGSAVWQLVASFLKLPISGTHCIVGATIGFSLVAKGQEGIKWSELIKIVMSWFVSPLLSGIMSGILFFLVRAFILRKADPVPNGLRALPIFYACTIGINLFSIMYTGAPLLGFDKLPLWGTILISVGCAVFCALIVWFFVCPRMKRKIEREVKSSPSESPLMEKKNNLKDHEETKMAPGDVENRNPVSEVVCATGPLRAVVEERTVSFKLGDLEEAPERERLPMDLKEETNIDGTINGAVQLPNGNLVQFSQTVSNQINSSGHYQYHTVHKDSGLYKELLHKLHLAKVGDCMGDSGDKPLRRNNSYTSYTMAICGMPLDSFRAKEGEQKGDEMETLTWPNADTKKRIRMDSYTSYCNAVSDLHSESEMDMSVKAEMGLGDRKGSSGSLEEWYDQDKPEVSLLFQFLQILTACFGSFAHGGNDVSNAIGPLVALYLVYETRDVTTKEATPIWLLLYGGVGICMGLWVWGRRVIQTMGKDLTPITPSSGFSIELASAFTVVVASNIGLPISTTHCKVGSVVSVGWLRSKKAVDWRLFRNIFMAWFVTVPISGVISAAIMAVFKHIILPV

Target ORF information:

RefSeq Version	XM_006234991.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 20 member 1 (Slc20a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234991.3

ORF Insert Sequence:

ATGGCATCTA CCCTGGCCCC GATTACTAGT ACTCTAGCTG CTGTTACTGC TTCTGCTCCA 
CCGAAGTATG ACAATCTGTG GATGCTCATC CTGGGCTTCA TCATTGCATT TGTCTTGGCA 
TTCTCCGTGG GAGCCAATGA TGTAGCAAAT TCATTTGGTA CAGCTGTAGG CTCAGGTGTA 
GTGACCCTGA AGCAAGCCTG CATCCTAGCT AGCATCTTCG AAACTGTGGG CTCCGCCTTG 
CTGGGGGCCA AAGTGAGTGA AACCATCCGG AAGGGCTTGA TAGATGTGGA GAAGTACAAC 
GCAACTCAAG ATCTGCTCAT GGCTGGCTCT GTCAGTGCTA TGTTTGGTTC TGCTGTGTGG 
CAACTAGTGG CTTCGTTTTT GAAGCTTCCT ATTTCTGGGA CCCATTGTAT TGTCGGTGCA 
ACCATTGGTT TCTCCCTTGT GGCAAAGGGA CAAGAGGGTA TCAAATGGTC CGAACTGATA 
AAAATTGTGA TGTCTTGGTT CGTCTCTCCA CTGCTTTCTG GTATTATGTC TGGAATTTTA 
TTCTTCCTTG TTCGTGCATT CATCCTCCGT AAGGCAGATC CAGTTCCTAA TGGCTTACGA 
GCTTTACCCA TTTTTTATGC CTGCACAATT GGAATCAACC TCTTTTCCAT TATGTATACT 
GGAGCACCAT TGCTGGGCTT TGACAAACTT CCTCTGTGGG GTACCATCCT CATCTCGGTG 
GGATGTGCAG TTTTCTGTGC CCTTATCGTC TGGTTCTTTG TATGTCCCAG GATGAAGAGG 
AAAATTGAAC GAGAAGTAAA GTCTAGTCCC TCTGAAAGTC CCTTAATGGA AAAGAAGAAC 
AACTTAAAAG ACCATGAAGA AACAAAGATG GCTCCTGGCG ATGTTGAGAA TAGGAATCCT 
GTGTCTGAGG TAGTATGTGC CACTGGCCCA CTACGGGCTG TGGTGGAGGA GAGAACGGTG 
TCATTTAAAC TCGGTGACCT GGAGGAAGCT CCGGAGCGAG AGCGGCTTCC CATGGACCTG 
AAGGAGGAGA CCAACATAGA TGGTACTATC AATGGTGCAG TGCAGTTGCC TAATGGGAAC 
CTTGTTCAGT TCAGTCAAAC CGTCAGCAAC CAGATCAACT CCAGTGGCCA CTATCAGTAT 
CACACCGTGC ACAAGGATTC TGGCTTGTAC AAAGAGCTGC TCCATAAGCT ACATCTGGCC 
AAGGTGGGAG ACTGCATGGG AGACTCTGGG GACAAGCCCT TGAGACGCAA CAACAGCTAC 
ACATCCTACA CCATGGCAAT ATGTGGCATG CCCCTGGATT CCTTCCGTGC CAAAGAAGGG 
GAACAAAAGG GAGACGAAAT GGAAACGCTG ACTTGGCCTA ATGCAGATAC CAAGAAGCGG 
ATTCGGATGG ACAGTTACAC CAGTTACTGC AATGCTGTGT CTGACCTTCA CTCAGAGTCT 
GAGATGGACA TGAGTGTTAA GGCAGAGATG GGTCTGGGTG ACAGAAAAGG AAGCAGTGGC 
TCTCTTGAAG AATGGTATGA TCAGGATAAG CCTGAAGTGT CCCTTCTCTT CCAGTTTCTG 
CAGATCCTTA CAGCCTGCTT TGGGTCCTTT GCCCATGGTG GCAATGACGT CAGCAATGCC 
ATCGGCCCTC TGGTTGCTTT GTATCTTGTT TATGAAACGA GAGATGTCAC TACAAAAGAG 
GCAACACCCA TATGGCTTCT GCTTTATGGT GGTGTTGGCA TTTGCATGGG GCTATGGGTT 
TGGGGAAGAA GAGTTATCCA GACTATGGGG AAGGACCTGA CACCAATCAC ACCCTCCAGT 
GGTTTCAGTA TTGAACTGGC ATCTGCCTTC ACTGTGGTCG TCGCATCAAA CATTGGCCTT 
CCCATCAGCA CAACACATTG TAAGGTGGGC TCTGTGGTGT CTGTTGGCTG GCTCCGATCC 
AAGAAGGCTG TTGACTGGCG ACTGTTTCGA AACATTTTTA TGGCCTGGTT TGTCACCGTC 
CCTATTTCTG GGGTTATCAG TGCTGCTATC ATGGCAGTGT TCAAGCACAT CATTCTGCCG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Magnesium prevents phosphate-induced vascular calcification via TRPM7 and Pit-1 in an aortic tissue culture model.
Hypertension research : official journal of the Japanese Society of Hypertension40(6)562-567(2017 Jun)
Sonou T,Ohya M,Yashiro M,Masumoto A,Nakashima Y,Ito T,Mima T,Negi S,Kimura-Suda H,Shigematsu T

Activating transcription factor-4 promotes mineralization in vascular smooth muscle cells.
JCI insight1(18)e88646(2016 Nov)
Masuda M,Miyazaki-Anzai S,Keenan AL,Shiozaki Y,Okamura K,Chick WS,Williams K,Zhao X,Rahman SM,Tintut Y,Adams CM,Miyazaki M

Effect of hyperphosphatemia on gene expression of the Na-Pi cotransporter in rats.
Genetics and molecular research : GMR14(4)19404-10(2016 Jan)
Zhang C,Shi LM,Li Y,Zhu QG,Jin CL,Wang HP,Zhou L,Hu XP,Zhang L,Wang H,Yuan GJ,Xu L,Zhao YK,Chou Y

Apelin: A novel inhibitor of vascular calcification in chronic kidney disease.
Atherosclerosis2441-8(2016 Jan)
Han X,Wang LY,Diao ZL,Liu WH

Phosphate handling: new genes, new molecules.
Hormone research in paediatrics76 Suppl 171-5(2011)
Pri? D,Torres PU,Friedlander G

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Slc20a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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