Ntf3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ntf3
Entrez Gene ID	81737
Full Name	neurotrophin 3
General protein information	Preferred Names neurotrophin 3 Names neurotrophin-3 HDNF NGF-2 NT-3 nerve growth factor 2 neurotrophic factor neurotrophin-3 (HDNF/NT-3)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q42
Summary	a neuronal survival protein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001270870.1	NP_001257799.1	neurotrophin-3 isoform 3 precursor
XM_008763295.1	XP_008761517.1	neurotrophin-3 isoform X1
NM_001270869.1	NP_001257798.1	neurotrophin-3 isoform 1
NM_031073.3	NP_112335.3	neurotrophin-3 isoform 1
NM_001270868.1	NP_001257797.1	neurotrophin-3 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ntf3	NP_001157507.1
Gallus gallus (chicken)	NTF3	NP_001103232.1
Homo sapiens (human)	NTF3	NP_001096124.1
Pan troglodytes (chimpanzee)	NTF3	XP_522331.2
Rattus norvegicus (Norway rat)	Ntf3	NP_001257798.1
Danio rerio (zebrafish)	ntf3	XP_697445.5
Xenopus tropicalis (tropical clawed frog)	ntf3	NP_001016370.1
	NTF3	XP_001118191.2
Canis lupus familiaris (dog)	NTF3	XP_003433523.1
Bos taurus (cattle)	NTF3	NP_001071456.1

Gene Ontology
Bibliography

Related articles in PubMed

Brain-derived neurotrophic factor/neurotrophin 3 regulate axon initial segment location and affect neuronal excitability in cultured hippocampal neurons.
Guo Y, Su ZJ, Chen YK, Chai Z
Journal of neurochemistry142(2)260-271(2017 Jul)

5-Aza-2'-deoxycytidine in the medial prefrontal cortex regulates alcohol-related behavior and Ntf3-TrkC expression in rats.
Qiao X, Yin F, Ji Y, Li Y, Yan P, Lai J
PloS one12(6)e0179469(2017)

[Effects of ischemia on the expression of neurotrophins and their receptors in rat brain structures outside the lesion site, including on the opposite hemisphere].
Dmitrieva VG, Stavchansky VV, Povarova OV, Skvortsova VI, Limborska SA, Dergunova LV
Molekuliarnaia biologiia50(5)775-784(2016 Sep-Oct)

Effects of Electroacupuncture at Governor Vessel Acupoints on Neurotrophin-3 in Rats with Experimental Spinal Cord Injury.
Mo YP, Yao HJ, Lv W, Song LY, Song HT, Yuan XC, Mao YQ, Jing QK, Shi SH, Li ZG
Neural plasticity20162371875(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate that BDNF and NT3 regulate axon initial segmen location and neuronal excitability
Title: Brain-derived neurotrophic factor/neurotrophin 3 regulate axon initial segment location and affect neuronal excitability in cultured hippocampal neurons.

Data show that 5-Aza-dc reversed the alcohol-induced downregulation of neurotrophin-3 (Ntf3), correspondingly the expression of its receptor-neurotrophic receptor tyrosine kinase 3 (TrkC) was reduced.
Title: 5-Aza-2'-deoxycytidine in the medial prefrontal cortex regulates alcohol-related behavior and Ntf3-TrkC expression in rats.

It has been shown that ischemia influenced the expression of neurotrophins (Bdnf, Ngf, Nt-3) and their receptors (TrkB, TrkA, TrkC, p75) in brain structures outside the lesion focus, including the contralateral hemisphere.
Title: [Effects of ischemia on the expression of neurotrophins and their receptors in rat brain structures outside the lesion site, including on the opposite hemisphere].

the combined delivery of NT-3 and anti-NogoA from the DDS in an impact/compression model of SCI increases axon density and improves locomotor function.
Title: Local Delivery of Neurotrophin-3 and Anti-NogoA Promotes Repair After Spinal Cord Injury.

Compared to the sham group, the expression levels of NT-3 were significantly decreased and electroacupuncture was shown to upregulate the expression of NT-3
Title: Effects of Electroacupuncture at Governor Vessel Acupoints on Neurotrophin-3 in Rats with Experimental Spinal Cord Injury.

The following Ntf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ntf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07520	NM_001270869.1 Latest version!	Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa07520	NM_031073.3 Latest version!	Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa09956	NM_001270870.1 Latest version!	Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa09947	NM_001270868.1 Latest version!	Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa09947	XM_008763295.1 Latest version!	Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ntf3 ( NM_001270869.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257798.1 Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa07520
Clone ID Related Accession (Same CDS sequence)	NM_001270869.1 , NM_031073.3
Accession Version	NM_001270869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	neurotrophin-3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07062046.1, FM101680.1, M34643.1 and BC070504.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FM101680.1, M34643.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTTACTT CTGCCACGAT CTTACAGGTG AACAAGGTGA TGTCCATCTT GTTTTATGTG 
ATATTTCTTG CTTATCTCCG TGGCATCCAA GGCAACAACA TGGATCAAAG GAGTTTGCCA 
GAAGACTCTC TCAATTCCCT CATTATCAAG TTGATCCAGG CGGATATCTT GAAAAACAAG 
CTCTCCAAGC AGATGGTAGA TGTTAAGGAA AATTACCAGA GCACCCTGCC CAAAGCAGAG 
GCACCCAGAG AACCAGAGCA GGGAGAGGCC ACCAGGTCAG AATTCCAGCC GATGATTGCA 
ACAGACACAG AACTACTACG GCAACAGAGA CGCTACAATT CACCCCGGGT CCTGCTGAGT 
GACAGCACCC CTTTGGAGCC CCCTCCCTTA TATCTAATGG AAGATTATGT GGGCAACCCG 
GTGGTAACCA ATAGAACATC ACCACGGAGG AAACGCTATG CAGAGCATAA GAGTCACCGA 
GGAGAGTACT CAGTGTGTGA CAGTGAGAGC CTGTGGGTGA CCGACAAGTC CTCAGCCATT 
GACATTCGGG GACACCAGGT TACAGTGTTG GGAGAGATCA AAACCGGCAA CTCTCCTGTG 
AAACAATATT TTTATGAAAC GAGGTGTAAA GAAGCCAGGC CAGTCAAAAA CGGTTGCAGG 
GGGATTGATG ACAAACACTG GAACTCTCAG TGCAAAACGT CGCAAACCTA CGTCCGAGCA 
CTGACTTCAG AAAACAACAA ACTCGTAGGC TGGCGCTGGA TACGAATAGA CACTTCCTGT 
GTGTGTGCCT TGTCAAGAAA AATCGGAAGA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257798.1
CDS	201..1016
Translation	MVTSATILQVNKVMSILFYVIFLAYLRGIQGNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPEQGEATRSEFQPMIATDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGNPVVTNRTSPRRKRYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

Target ORF information:

RefSeq Version	NM_001270869.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270869.1

ORF Insert Sequence:

ATGGTTACTT CTGCCACGAT CTTACAGGTG AACAAGGTGA TGTCCATCTT GTTTTATGTG 
ATATTTCTTG CTTATCTCCG TGGCATCCAA GGCAACAACA TGGATCAAAG GAGTTTGCCA 
GAAGACTCTC TCAATTCCCT CATTATCAAG TTGATCCAGG CGGATATCTT GAAAAACAAG 
CTCTCCAAGC AGATGGTAGA TGTTAAGGAA AATTACCAGA GCACCCTGCC CAAAGCAGAG 
GCACCCAGAG AACCAGAGCA GGGAGAGGCC ACCAGGTCAG AATTCCAGCC GATGATTGCA 
ACAGACACAG AACTACTACG GCAACAGAGA CGCTACAATT CACCCCGGGT CCTGCTGAGT 
GACAGCACCC CTTTGGAGCC CCCTCCCTTA TATCTAATGG AAGATTATGT GGGCAACCCG 
GTGGTAACCA ATAGAACATC ACCACGGAGG AAACGCTATG CAGAGCATAA GAGTCACCGA 
GGAGAGTACT CAGTGTGTGA CAGTGAGAGC CTGTGGGTGA CCGACAAGTC CTCAGCCATT 
GACATTCGGG GACACCAGGT TACAGTGTTG GGAGAGATCA AAACCGGCAA CTCTCCTGTG 
AAACAATATT TTTATGAAAC GAGGTGTAAA GAAGCCAGGC CAGTCAAAAA CGGTTGCAGG 
GGGATTGATG ACAAACACTG GAACTCTCAG TGCAAAACGT CGCAAACCTA CGTCCGAGCA 
CTGACTTCAG AAAACAACAA ACTCGTAGGC TGGCGCTGGA TACGAATAGA CACTTCCTGT 
GTGTGTGCCT TGTCAAGAAA AATCGGAAGA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ntf3 ( NM_031073.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112335.3 Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa07520
Clone ID Related Accession (Same CDS sequence)	NM_001270869.1 , NM_031073.3
Accession Version	NM_031073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	neurotrophin-3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC070504.1 and M34643.1. On Aug 4, 2012 this sequence version replaced NM_031073.2. Transcript Variant: This variant (1) is the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070504.1, CK365049.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTTACTT CTGCCACGAT CTTACAGGTG AACAAGGTGA TGTCCATCTT GTTTTATGTG 
ATATTTCTTG CTTATCTCCG TGGCATCCAA GGCAACAACA TGGATCAAAG GAGTTTGCCA 
GAAGACTCTC TCAATTCCCT CATTATCAAG TTGATCCAGG CGGATATCTT GAAAAACAAG 
CTCTCCAAGC AGATGGTAGA TGTTAAGGAA AATTACCAGA GCACCCTGCC CAAAGCAGAG 
GCACCCAGAG AACCAGAGCA GGGAGAGGCC ACCAGGTCAG AATTCCAGCC GATGATTGCA 
ACAGACACAG AACTACTACG GCAACAGAGA CGCTACAATT CACCCCGGGT CCTGCTGAGT 
GACAGCACCC CTTTGGAGCC CCCTCCCTTA TATCTAATGG AAGATTATGT GGGCAACCCG 
GTGGTAACCA ATAGAACATC ACCACGGAGG AAACGCTATG CAGAGCATAA GAGTCACCGA 
GGAGAGTACT CAGTGTGTGA CAGTGAGAGC CTGTGGGTGA CCGACAAGTC CTCAGCCATT 
GACATTCGGG GACACCAGGT TACAGTGTTG GGAGAGATCA AAACCGGCAA CTCTCCTGTG 
AAACAATATT TTTATGAAAC GAGGTGTAAA GAAGCCAGGC CAGTCAAAAA CGGTTGCAGG 
GGGATTGATG ACAAACACTG GAACTCTCAG TGCAAAACGT CGCAAACCTA CGTCCGAGCA 
CTGACTTCAG AAAACAACAA ACTCGTAGGC TGGCGCTGGA TACGAATAGA CACTTCCTGT 
GTGTGTGCCT TGTCAAGAAA AATCGGAAGA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112335.3
CDS	283..1098
Translation	MVTSATILQVNKVMSILFYVIFLAYLRGIQGNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPEQGEATRSEFQPMIATDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGNPVVTNRTSPRRKRYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

Target ORF information:

RefSeq Version	NM_031073.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031073.3

ORF Insert Sequence:

ATGGTTACTT CTGCCACGAT CTTACAGGTG AACAAGGTGA TGTCCATCTT GTTTTATGTG 
ATATTTCTTG CTTATCTCCG TGGCATCCAA GGCAACAACA TGGATCAAAG GAGTTTGCCA 
GAAGACTCTC TCAATTCCCT CATTATCAAG TTGATCCAGG CGGATATCTT GAAAAACAAG 
CTCTCCAAGC AGATGGTAGA TGTTAAGGAA AATTACCAGA GCACCCTGCC CAAAGCAGAG 
GCACCCAGAG AACCAGAGCA GGGAGAGGCC ACCAGGTCAG AATTCCAGCC GATGATTGCA 
ACAGACACAG AACTACTACG GCAACAGAGA CGCTACAATT CACCCCGGGT CCTGCTGAGT 
GACAGCACCC CTTTGGAGCC CCCTCCCTTA TATCTAATGG AAGATTATGT GGGCAACCCG 
GTGGTAACCA ATAGAACATC ACCACGGAGG AAACGCTATG CAGAGCATAA GAGTCACCGA 
GGAGAGTACT CAGTGTGTGA CAGTGAGAGC CTGTGGGTGA CCGACAAGTC CTCAGCCATT 
GACATTCGGG GACACCAGGT TACAGTGTTG GGAGAGATCA AAACCGGCAA CTCTCCTGTG 
AAACAATATT TTTATGAAAC GAGGTGTAAA GAAGCCAGGC CAGTCAAAAA CGGTTGCAGG 
GGGATTGATG ACAAACACTG GAACTCTCAG TGCAAAACGT CGCAAACCTA CGTCCGAGCA 
CTGACTTCAG AAAACAACAA ACTCGTAGGC TGGCGCTGGA TACGAATAGA CACTTCCTGT 
GTGTGTGCCT TGTCAAGAAA AATCGGAAGA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ntf3 ( NM_001270870.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257799.1 Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	ORa09956
Clone ID Related Accession (Same CDS sequence)	NM_001270870.1
Accession Version	NM_001270870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	neurotrophin-3 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EV778881.1, BC070504.1 and M34643.1. Transcript Variant: This variant (4) lacks an exon in the 5' region, which causes translation initiation at a downstream AUG, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EV778881.1, EV780605.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGTCCATCT TGTTTTATGT GATATTTCTT GCTTATCTCC GTGGCATCCA AGGCAACAAC 
ATGGATCAAA GGAGTTTGCC AGAAGACTCT CTCAATTCCC TCATTATCAA GTTGATCCAG 
GCGGATATCT TGAAAAACAA GCTCTCCAAG CAGATGGTAG ATGTTAAGGA AAATTACCAG 
AGCACCCTGC CCAAAGCAGA GGCACCCAGA GAACCAGAGC AGGGAGAGGC CACCAGGTCA 
GAATTCCAGC CGATGATTGC AACAGACACA GAACTACTAC GGCAACAGAG ACGCTACAAT 
TCACCCCGGG TCCTGCTGAG TGACAGCACC CCTTTGGAGC CCCCTCCCTT ATATCTAATG 
GAAGATTATG TGGGCAACCC GGTGGTAACC AATAGAACAT CACCACGGAG GAAACGCTAT 
GCAGAGCATA AGAGTCACCG AGGAGAGTAC TCAGTGTGTG ACAGTGAGAG CCTGTGGGTG 
ACCGACAAGT CCTCAGCCAT TGACATTCGG GGACACCAGG TTACAGTGTT GGGAGAGATC 
AAAACCGGCA ACTCTCCTGT GAAACAATAT TTTTATGAAA CGAGGTGTAA AGAAGCCAGG 
CCAGTCAAAA ACGGTTGCAG GGGGATTGAT GACAAACACT GGAACTCTCA GTGCAAAACG 
TCGCAAACCT ACGTCCGAGC ACTGACTTCA GAAAACAACA AACTCGTAGG CTGGCGCTGG 
ATACGAATAG ACACTTCCTG TGTGTGTGCC TTGTCAAGAA AAATCGGAAG AACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257799.1
CDS	212..988
Translation	MSILFYVIFLAYLRGIQGNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPEQGEATRSEFQPMIATDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGNPVVTNRTSPRRKRYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

Target ORF information:

RefSeq Version	NM_001270870.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270870.1

ORF Insert Sequence:

ATGTCCATCT TGTTTTATGT GATATTTCTT GCTTATCTCC GTGGCATCCA AGGCAACAAC 
ATGGATCAAA GGAGTTTGCC AGAAGACTCT CTCAATTCCC TCATTATCAA GTTGATCCAG 
GCGGATATCT TGAAAAACAA GCTCTCCAAG CAGATGGTAG ATGTTAAGGA AAATTACCAG 
AGCACCCTGC CCAAAGCAGA GGCACCCAGA GAACCAGAGC AGGGAGAGGC CACCAGGTCA 
GAATTCCAGC CGATGATTGC AACAGACACA GAACTACTAC GGCAACAGAG ACGCTACAAT 
TCACCCCGGG TCCTGCTGAG TGACAGCACC CCTTTGGAGC CCCCTCCCTT ATATCTAATG 
GAAGATTATG TGGGCAACCC GGTGGTAACC AATAGAACAT CACCACGGAG GAAACGCTAT 
GCAGAGCATA AGAGTCACCG AGGAGAGTAC TCAGTGTGTG ACAGTGAGAG CCTGTGGGTG 
ACCGACAAGT CCTCAGCCAT TGACATTCGG GGACACCAGG TTACAGTGTT GGGAGAGATC 
AAAACCGGCA ACTCTCCTGT GAAACAATAT TTTTATGAAA CGAGGTGTAA AGAAGCCAGG 
CCAGTCAAAA ACGGTTGCAG GGGGATTGAT GACAAACACT GGAACTCTCA GTGCAAAACG 
TCGCAAACCT ACGTCCGAGC ACTGACTTCA GAAAACAACA AACTCGTAGG CTGGCGCTGG 
ATACGAATAG ACACTTCCTG TGTGTGTGCC TTGTCAAGAA AAATCGGAAG AACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ntf3 ( NM_001270868.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257797.1 Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa09947
Clone ID Related Accession (Same CDS sequence)	XM_008763295.1 , NM_001270868.1
Accession Version	NM_001270868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	neurotrophin-3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07062046.1, FM074856.1, M34643.1 and BC070504.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) is of the same size but has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FM074856.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN01906353 [ECO:0000348] ##Evidence-Data-END##

ATGCTGGGCT TCCTGAAGAT CTTACAGGTG AACAAGGTGA TGTCCATCTT GTTTTATGTG 
ATATTTCTTG CTTATCTCCG TGGCATCCAA GGCAACAACA TGGATCAAAG GAGTTTGCCA 
GAAGACTCTC TCAATTCCCT CATTATCAAG TTGATCCAGG CGGATATCTT GAAAAACAAG 
CTCTCCAAGC AGATGGTAGA TGTTAAGGAA AATTACCAGA GCACCCTGCC CAAAGCAGAG 
GCACCCAGAG AACCAGAGCA GGGAGAGGCC ACCAGGTCAG AATTCCAGCC GATGATTGCA 
ACAGACACAG AACTACTACG GCAACAGAGA CGCTACAATT CACCCCGGGT CCTGCTGAGT 
GACAGCACCC CTTTGGAGCC CCCTCCCTTA TATCTAATGG AAGATTATGT GGGCAACCCG 
GTGGTAACCA ATAGAACATC ACCACGGAGG AAACGCTATG CAGAGCATAA GAGTCACCGA 
GGAGAGTACT CAGTGTGTGA CAGTGAGAGC CTGTGGGTGA CCGACAAGTC CTCAGCCATT 
GACATTCGGG GACACCAGGT TACAGTGTTG GGAGAGATCA AAACCGGCAA CTCTCCTGTG 
AAACAATATT TTTATGAAAC GAGGTGTAAA GAAGCCAGGC CAGTCAAAAA CGGTTGCAGG 
GGGATTGATG ACAAACACTG GAACTCTCAG TGCAAAACGT CGCAAACCTA CGTCCGAGCA 
CTGACTTCAG AAAACAACAA ACTCGTAGGC TGGCGCTGGA TACGAATAGA CACTTCCTGT 
GTGTGTGCCT TGTCAAGAAA AATCGGAAGA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257797.1
CDS	239..1054
Translation	MLGFLKILQVNKVMSILFYVIFLAYLRGIQGNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPEQGEATRSEFQPMIATDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGNPVVTNRTSPRRKRYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

Target ORF information:

RefSeq Version	NM_001270868.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270868.1

ORF Insert Sequence:

ATGCTGGGCT TCCTGAAGAT CTTACAGGTG AACAAGGTGA TGTCCATCTT GTTTTATGTG 
ATATTTCTTG CTTATCTCCG TGGCATCCAA GGCAACAACA TGGATCAAAG GAGTTTGCCA 
GAAGACTCTC TCAATTCCCT CATTATCAAG TTGATCCAGG CGGATATCTT GAAAAACAAG 
CTCTCCAAGC AGATGGTAGA TGTTAAGGAA AATTACCAGA GCACCCTGCC CAAAGCAGAG 
GCACCCAGAG AACCAGAGCA GGGAGAGGCC ACCAGGTCAG AATTCCAGCC GATGATTGCA 
ACAGACACAG AACTACTACG GCAACAGAGA CGCTACAATT CACCCCGGGT CCTGCTGAGT 
GACAGCACCC CTTTGGAGCC CCCTCCCTTA TATCTAATGG AAGATTATGT GGGCAACCCG 
GTGGTAACCA ATAGAACATC ACCACGGAGG AAACGCTATG CAGAGCATAA GAGTCACCGA 
GGAGAGTACT CAGTGTGTGA CAGTGAGAGC CTGTGGGTGA CCGACAAGTC CTCAGCCATT 
GACATTCGGG GACACCAGGT TACAGTGTTG GGAGAGATCA AAACCGGCAA CTCTCCTGTG 
AAACAATATT TTTATGAAAC GAGGTGTAAA GAAGCCAGGC CAGTCAAAAA CGGTTGCAGG 
GGGATTGATG ACAAACACTG GAACTCTCAG TGCAAAACGT CGCAAACCTA CGTCCGAGCA 
CTGACTTCAG AAAACAACAA ACTCGTAGGC TGGCGCTGGA TACGAATAGA CACTTCCTGT 
GTGTGTGCCT TGTCAAGAAA AATCGGAAGA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ntf3 ( XM_008763295.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761517.1 Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant X1, mRNA.

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CloneID	ORa09947
Clone ID Related Accession (Same CDS sequence)	XM_008763295.1 , NM_001270868.1
Accession Version	XM_008763295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurotrophin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGCT TCCTGAAGAT CTTACAGGTG AACAAGGTGA TGTCCATCTT GTTTTATGTG 
ATATTTCTTG CTTATCTCCG TGGCATCCAA GGCAACAACA TGGATCAAAG GAGTTTGCCA 
GAAGACTCTC TCAATTCCCT CATTATCAAG TTGATCCAGG CGGATATCTT GAAAAACAAG 
CTCTCCAAGC AGATGGTAGA TGTTAAGGAA AATTACCAGA GCACCCTGCC CAAAGCAGAG 
GCACCCAGAG AACCAGAGCA GGGAGAGGCC ACCAGGTCAG AATTCCAGCC GATGATTGCA 
ACAGACACAG AACTACTACG GCAACAGAGA CGCTACAATT CACCCCGGGT CCTGCTGAGT 
GACAGCACCC CTTTGGAGCC CCCTCCCTTA TATCTAATGG AAGATTATGT GGGCAACCCG 
GTGGTAACCA ATAGAACATC ACCACGGAGG AAACGCTATG CAGAGCATAA GAGTCACCGA 
GGAGAGTACT CAGTGTGTGA CAGTGAGAGC CTGTGGGTGA CCGACAAGTC CTCAGCCATT 
GACATTCGGG GACACCAGGT TACAGTGTTG GGAGAGATCA AAACCGGCAA CTCTCCTGTG 
AAACAATATT TTTATGAAAC GAGGTGTAAA GAAGCCAGGC CAGTCAAAAA CGGTTGCAGG 
GGGATTGATG ACAAACACTG GAACTCTCAG TGCAAAACGT CGCAAACCTA CGTCCGAGCA 
CTGACTTCAG AAAACAACAA ACTCGTAGGC TGGCGCTGGA TACGAATAGA CACTTCCTGT 
GTGTGTGCCT TGTCAAGAAA AATCGGAAGA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761517.1
CDS	235..1050
Translation	MLGFLKILQVNKVMSILFYVIFLAYLRGIQGNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPEQGEATRSEFQPMIATDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGNPVVTNRTSPRRKRYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

Target ORF information:

RefSeq Version	XM_008763295.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurotrophin 3 (Ntf3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763295.1

ORF Insert Sequence:

ATGCTGGGCT TCCTGAAGAT CTTACAGGTG AACAAGGTGA TGTCCATCTT GTTTTATGTG 
ATATTTCTTG CTTATCTCCG TGGCATCCAA GGCAACAACA TGGATCAAAG GAGTTTGCCA 
GAAGACTCTC TCAATTCCCT CATTATCAAG TTGATCCAGG CGGATATCTT GAAAAACAAG 
CTCTCCAAGC AGATGGTAGA TGTTAAGGAA AATTACCAGA GCACCCTGCC CAAAGCAGAG 
GCACCCAGAG AACCAGAGCA GGGAGAGGCC ACCAGGTCAG AATTCCAGCC GATGATTGCA 
ACAGACACAG AACTACTACG GCAACAGAGA CGCTACAATT CACCCCGGGT CCTGCTGAGT 
GACAGCACCC CTTTGGAGCC CCCTCCCTTA TATCTAATGG AAGATTATGT GGGCAACCCG 
GTGGTAACCA ATAGAACATC ACCACGGAGG AAACGCTATG CAGAGCATAA GAGTCACCGA 
GGAGAGTACT CAGTGTGTGA CAGTGAGAGC CTGTGGGTGA CCGACAAGTC CTCAGCCATT 
GACATTCGGG GACACCAGGT TACAGTGTTG GGAGAGATCA AAACCGGCAA CTCTCCTGTG 
AAACAATATT TTTATGAAAC GAGGTGTAAA GAAGCCAGGC CAGTCAAAAA CGGTTGCAGG 
GGGATTGATG ACAAACACTG GAACTCTCAG TGCAAAACGT CGCAAACCTA CGTCCGAGCA 
CTGACTTCAG AAAACAACAA ACTCGTAGGC TGGCGCTGGA TACGAATAGA CACTTCCTGT 
GTGTGTGCCT TGTCAAGAAA AATCGGAAGA ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Brain-derived neurotrophic factor/neurotrophin 3 regulate axon initial segment location and affect neuronal excitability in cultured hippocampal neurons.
Journal of neurochemistry142(2)260-271(2017 Jul)
Guo Y,Su ZJ,Chen YK,Chai Z

5-Aza-2'-deoxycytidine in the medial prefrontal cortex regulates alcohol-related behavior and Ntf3-TrkC expression in rats.
PloS one12(6)e0179469(2017)
Qiao X,Yin F,Ji Y,Li Y,Yan P,Lai J

[Effects of ischemia on the expression of neurotrophins and their receptors in rat brain structures outside the lesion site, including on the opposite hemisphere].
Molekuliarnaia biologiia50(5)775-784(2016 Sep-Oct)
Dmitrieva VG,Stavchansky VV,Povarova OV,Skvortsova VI,Limborska SA,Dergunova LV

Effects of Electroacupuncture at Governor Vessel Acupoints on Neurotrophin-3 in Rats with Experimental Spinal Cord Injury.
Neural plasticity20162371875(2016)
Mo YP,Yao HJ,Lv W,Song LY,Song HT,Yuan XC,Mao YQ,Jing QK,Shi SH,Li ZG

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Ntf3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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