Nell2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nell2
Entrez Gene ID	81734
Full Name	neural EGFL like 2
General protein information	Preferred Names neural EGFL like 2 Names protein kinase C-binding protein NELL2 NEL-like 2 NEL-like protein 2 rCG50753-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q35
Summary	homotrimeric glycoprotein that is involved in the growth and differentiation of neural cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008765797.2	XP_008764019.1	protein kinase C-binding protein NELL2 isoform X1
NM_031070.2	NP_112332.2	protein kinase C-binding protein NELL2 precursor
XM_008765798.2	XP_008764020.1	protein kinase C-binding protein NELL2 isoform X1

Pathways from BioSystems

Homologies

	NELL2	XP_001091444.1
Bos taurus (cattle)	NELL2	NP_001095554.1
Homo sapiens (human)	NELL2	NP_001138579.1
Pan troglodytes (chimpanzee)	NELL2	XP_003313795.1
Canis lupus familiaris (dog)	NELL2	XP_005637011.1
Xenopus tropicalis (tropical clawed frog)	nell2	NP_001090754.1
Mus musculus (house mouse)	Nell2	NP_058023.2
Rattus norvegicus (Norway rat)	Nell2	NP_112332.2
Gallus gallus (chicken)	NELL2	NP_001025911.1
Danio rerio (zebrafish)	nell2b	XP_002661425.1

Gene Ontology
Bibliography

Related articles in PubMed

A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats.
Jeong JK, Kim JG, Kim HR, Lee TH, Park JW, Lee BJ
Molecules and cells40(3)186-194(2017 Mar)

The molecular mechanism of NELL2 movement and secretion in hippocampal progenitor HiB5 cells.
Ha CM, Hwang EM, Kim E, Lee DY, Chang S, Lee BJ, Hong SG, Park JY
Molecules and cells36(6)527-33(2013 Dec)

The neuronal EGF-related gene Nell2 interacts with Macf1 and supports survival of retinal ganglion cells after optic nerve injury.
Munemasa Y, Chang CS, Kwong JM, Kyung H, Kitaoka Y, Caprioli J, Piri N
PloS one7(4)e34810(2012)

Regulation of the female rat estrous cycle by a neural cell-specific epidermal growth factor-like repeat domain containing protein, NELL2.
Ryu BJ, Kim HR, Jeong JK, Lee BJ
Molecules and cells32(2)203-7(2011 Aug)

A neuron-specific EGF family protein, NELL2, promotes survival of neurons through mitogen-activated protein kinases.
Aihara K, Kuroda S, Kanayama N, Matsuyama S, Tanizawa K, Horie M
Brain research. Molecular brain research116(1-2)86-93(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report shows that neural tissue-enriched multifunctional protein, NELL2 is a regulatory component of feeding behavior, possibly through a modulation of neuronal activity in the hypothalamus.
Title: A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats.

Data indicate that RNA interference of NEL-like protein 2 (NELL2) reduced NELL2 and gonadotropin-releasing hormone (GnRH) expression at multiple stages of sexual development.
Title: Effects of NELL2 on the regulation of GnRH expression and puberty in female rats.

Results suggest that the N-terminal region of NELL2 determines both the pattern of its intracellular expression and transport and may affect the cellular activity of cells in a paracrine or autocrine manner.
Title: The molecular mechanism of NELL2 movement and secretion in hippocampal progenitor HiB5 cells.

It was shown that Nell2 expression at both mRNA and protein levels is localized to retinal ganglion cells. Nell2 had a molecular weight of ~ 140 kDa and 90 kDa, corresponding to the glycosylated and non-glycosylated form, respectively.
Title: The neuronal EGF-related gene Nell2 interacts with Macf1 and supports survival of retinal ganglion cells after optic nerve injury.

involved in the maintenance of the normal female reproductive cycle
Title: Regulation of the female rat estrous cycle by a neural cell-specific epidermal growth factor-like repeat domain containing protein, NELL2.

The following Nell2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nell2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07515	NM_031070.2 Latest version!	Rattus norvegicus neural EGFL like 2 (Nell2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa07515	XM_008765797.2 Latest version!	Rattus norvegicus neural EGFL like 2 (Nell2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa07515	XM_008765798.2 Latest version!	Rattus norvegicus neural EGFL like 2 (Nell2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nell2 ( NM_031070.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112332.2 Rattus norvegicus neural EGFL like 2 (Nell2), mRNA.

Price & Availability↑

CloneID	ORa07515
Clone ID Related Accession (Same CDS sequence)	XM_008765797.2 , NM_031070.2 , XM_008765798.2
Accession Version	NM_031070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C-binding protein NELL2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY089719.1 and BC093617.1. On Oct 14, 2005 this sequence version replaced NM_031070.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY089719.1, U48245.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCACGCCA TGGAATCCCG GGTATTACTG AGAACGTTCT GCGTGATCCT CGGGCTCGAA 
GCGGTTTGGG GACTTGGTGT GGACCCCTCC CTACAGATTG ACGTCTTATC AGAGTTAGAA 
CTTGGGGAGT CCACAGCTGG AGTGCGCCAA GTCCCAGGAC TGCATAATGG GACGAAAGCC 
TTCCTCTTCC AAGATTCTCC CAGAAGCATA AAAGCACCCA TTGCTACAGC TGAGCGGTTT 
TTCCAGAAGC TGAGGAATAA ACACGAGTTC ACAATTCTGG TGACCCTGAA ACAGATCCAC 
TTAAATTCGG GAGTCATTCT CTCCATCCAC CACTTGGATC ACAGGTACCT GGAACTGGAA 
AGCAGCGGCC ACCGGAATGA GATCAGACTG CATTACCGCT CTGGAACTCA CCGCCCGCAC 
ACGGAAGTGT TTCCTTACAT TTTGGCTGAT GCCAAGTGGC ACAAGCTCTC CTTAGCCTTC 
AGTGCCTCCC ACTTAATTTT ACACATCGAC TGCAACAAGA TCTATGAACG AGTGGTGGAA 
ATGCCTTCTA CAGACTTGCC TCTGGGCACC ACATTTTGGT TGGGACAGAG AAATAACGCA 
CACGGGTATT TTAAGGGAAT AATGCAAGAT GTGCAATTAC TTGTCATGCC CCAGGGGTTC 
ATCGCTCAGT GCCCGGATCT TAATCGAACC TGTCCAACAT GCAACGACTT CCATGGGCTT 
GTGCAGAAAA TCATGGAGCT GCAGGACATT TTATCGAAGA CGTCAGCCAA GTTGTCTAGA 
GCTGAACAAC GAATGAACAG GCTGGATCAG TGCTACTGTG AGCGGACGTG CACCATGAAG 
GGAACCACCT ACCGGGAGTT CGAGTCCTGG ACAGACGGCT GCAAGAACTG CACATGCTTG 
AATGGGACCA TCCAGTGCGA GACTCTGGTC TGCCCTGCTC CCGACTGCCC GGCTAAATCG 
GCTCCAGCGT ACGTGGATGG CAAGTGCTGT AAGGAGTGCA AGTCCACCTG CCAGTTCCAG 
GGGCGGAGCT ACTTTGAGGG AGAAAGGAGC ACAGTCTTCT CAGCTTCCGG AATGTGCGTC 
TTGTATGAAT GCAAGGATCA GACCATGAAG CTTGTTGAGA ACGCCGGCTG CCCGGCTTTA 
GATTGCCCCG AGTCTCATCA GATCGCCTTG TCTCACAGCT GCTGCAAGGT TTGCAAAGGT 
TATGACTTCT GTTCTGAGAA GCATACATGC ATGGAGAACT CAGTCTGCAG GAACCTGAAC 
GACAGGGCAG TGTGCAGCTG CCGGGATGGT TTCCGGGCCC TCCGGGAGGA CAATGCCTAC 
TGTGAAGACA TTGACGAGTG TGCAGAGGGG CGCCATTACT GCCGTGAGAA CACCATGTGT 
GTGAACACAC CGGGCTCTTT CCTGTGTATC TGCCAAACAG GGTACATCAG AATCGACGAT 
TACTCGTGTA CGGAACATGA CGAGTGCCTC ACAAACCAGC ACAATTGTGA CGAGAACGCT 
TTGTGCTTTA ACACCGTTGG AGGTCACAAC TGCGTCTGCA AGCCTGGCTA CACTGGGAAT 
GGAACCACGT GCAAAGCTTT CTGCAAAGAC GGCTGCAGAA ACGGAGGTGC CTGCATTGCT 
GCCAATGTCT GTGCTTGCCC ACAAGGCTTC ACCGGACCCA GCTGTGAGAC AGACATTGAT 
GAGTGCTCTG AGGGCTTTGT TCAGTGTGAC AGCCGTGCCA ACTGCATTAA CCTGCCTGGG 
TGGTACCACT GTGAGTGCAG AGATGGCTAC CATGACAATG GGATGTTTGC ACCAGGTGGA 
GAATCCTGTG AAGATATTGA TGAATGTGGG ACTGGGAGGC ACAGCTGTGC CAATGACACC 
ATTTGCTTCA ACTTGGACGG TGGCTACGAT TGCCGGTGTC CCCATGGAAA GAACTGCACA 
GGGGACTGCG TGCACGACGG GAAAGTCAAA CACAACGGCC AGATCTGGGT GCTGGAGAAC 
GACAGGTGCT CTGTGTGTTC CTGCCAGACT GGATTTGTTA TGTGTCGACG GATGGTCTGT 
GACTGCGAAA ACCCCACAGT TGACCTCTCC TGCTGCCCTG AGTGCGACCC AAGGCTGAGC 
AGCCAGTGCC TGCATCAAAA CGGGGAAACC GTGTACAACA GCGGTGACAC CTGGGTCCAG 
GATTGCCGTC AGTGCCGCTG CTTGCAAGGA GAAGTTGACT GCTGGCCCCT GGCTTGCCCA 
GAGGTAGAGT GTGAATTTAG TGTCCTTCCT GAGAACGAGT GCTGCCCACG CTGTGTCACC 
GATCCTTGTC AGGCTGACAC CATCCGCAAT GACATCACCA AAACCTGCCT GGACGAGATG 
AACGTGGTTC GCTTCACTGG GTCTTCCTGG ATCAAGCACG GCACAGAGTG CACCCTCTGC 
CAGTGCAAGA ACGGCCACGT GTGCTGCTCA GTGGACCCAC AGTGCCTCCA GGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112332.2
CDS	70..2529
Translation	MHAMESRVLLRTFCVILGLEAVWGLGVDPSLQIDVLSELELGESTAGVRQVPGLHNGTKAFLFQDSPRSIKAPIATAERFFQKLRNKHEFTILVTLKQIHLNSGVILSIHHLDHRYLELESSGHRNEIRLHYRSGTHRPHTEVFPYILADAKWHKLSLAFSASHLILHIDCNKIYERVVEMPSTDLPLGTTFWLGQRNNAHGYFKGIMQDVQLLVMPQGFIAQCPDLNRTCPTCNDFHGLVQKIMELQDILSKTSAKLSRAEQRMNRLDQCYCERTCTMKGTTYREFESWTDGCKNCTCLNGTIQCETLVCPAPDCPAKSAPAYVDGKCCKECKSTCQFQGRSYFEGERSTVFSASGMCVLYECKDQTMKLVENAGCPALDCPESHQIALSHSCCKVCKGYDFCSEKHTCMENSVCRNLNDRAVCSCRDGFRALREDNAYCEDIDECAEGRHYCRENTMCVNTPGSFLCICQTGYIRIDDYSCTEHDECLTNQHNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSEGFVQCDSRANCINLPGWYHCECRDGYHDNGMFAPGGESCEDIDECGTGRHSCANDTICFNLDGGYDCRCPHGKNCTGDCVHDGKVKHNGQIWVLENDRCSVCSCQTGFVMCRRMVCDCENPTVDLSCCPECDPRLSSQCLHQNGETVYNSGDTWVQDCRQCRCLQGEVDCWPLACPEVECEFSVLPENECCPRCVTDPCQADTIRNDITKTCLDEMNVVRFTGSSWIKHGTECTLCQCKNGHVCCSVDPQCLQEL

Target ORF information:

RefSeq Version	NM_031070.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neural EGFL like 2 (Nell2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031070.2

ORF Insert Sequence:

ATGCACGCCA TGGAATCCCG GGTATTACTG AGAACGTTCT GCGTGATCCT CGGGCTCGAA 
GCGGTTTGGG GACTTGGTGT GGACCCCTCC CTACAGATTG ACGTCTTATC AGAGTTAGAA 
CTTGGGGAGT CCACAGCTGG AGTGCGCCAA GTCCCAGGAC TGCATAATGG GACGAAAGCC 
TTCCTCTTCC AAGATTCTCC CAGAAGCATA AAAGCACCCA TTGCTACAGC TGAGCGGTTT 
TTCCAGAAGC TGAGGAATAA ACACGAGTTC ACAATTCTGG TGACCCTGAA ACAGATCCAC 
TTAAATTCGG GAGTCATTCT CTCCATCCAC CACTTGGATC ACAGGTACCT GGAACTGGAA 
AGCAGCGGCC ACCGGAATGA GATCAGACTG CATTACCGCT CTGGAACTCA CCGCCCGCAC 
ACGGAAGTGT TTCCTTACAT TTTGGCTGAT GCCAAGTGGC ACAAGCTCTC CTTAGCCTTC 
AGTGCCTCCC ACTTAATTTT ACACATCGAC TGCAACAAGA TCTATGAACG AGTGGTGGAA 
ATGCCTTCTA CAGACTTGCC TCTGGGCACC ACATTTTGGT TGGGACAGAG AAATAACGCA 
CACGGGTATT TTAAGGGAAT AATGCAAGAT GTGCAATTAC TTGTCATGCC CCAGGGGTTC 
ATCGCTCAGT GCCCGGATCT TAATCGAACC TGTCCAACAT GCAACGACTT CCATGGGCTT 
GTGCAGAAAA TCATGGAGCT GCAGGACATT TTATCGAAGA CGTCAGCCAA GTTGTCTAGA 
GCTGAACAAC GAATGAACAG GCTGGATCAG TGCTACTGTG AGCGGACGTG CACCATGAAG 
GGAACCACCT ACCGGGAGTT CGAGTCCTGG ACAGACGGCT GCAAGAACTG CACATGCTTG 
AATGGGACCA TCCAGTGCGA GACTCTGGTC TGCCCTGCTC CCGACTGCCC GGCTAAATCG 
GCTCCAGCGT ACGTGGATGG CAAGTGCTGT AAGGAGTGCA AGTCCACCTG CCAGTTCCAG 
GGGCGGAGCT ACTTTGAGGG AGAAAGGAGC ACAGTCTTCT CAGCTTCCGG AATGTGCGTC 
TTGTATGAAT GCAAGGATCA GACCATGAAG CTTGTTGAGA ACGCCGGCTG CCCGGCTTTA 
GATTGCCCCG AGTCTCATCA GATCGCCTTG TCTCACAGCT GCTGCAAGGT TTGCAAAGGT 
TATGACTTCT GTTCTGAGAA GCATACATGC ATGGAGAACT CAGTCTGCAG GAACCTGAAC 
GACAGGGCAG TGTGCAGCTG CCGGGATGGT TTCCGGGCCC TCCGGGAGGA CAATGCCTAC 
TGTGAAGACA TTGACGAGTG TGCAGAGGGG CGCCATTACT GCCGTGAGAA CACCATGTGT 
GTGAACACAC CGGGCTCTTT CCTGTGTATC TGCCAAACAG GGTACATCAG AATCGACGAT 
TACTCGTGTA CGGAACATGA CGAGTGCCTC ACAAACCAGC ACAATTGTGA CGAGAACGCT 
TTGTGCTTTA ACACCGTTGG AGGTCACAAC TGCGTCTGCA AGCCTGGCTA CACTGGGAAT 
GGAACCACGT GCAAAGCTTT CTGCAAAGAC GGCTGCAGAA ACGGAGGTGC CTGCATTGCT 
GCCAATGTCT GTGCTTGCCC ACAAGGCTTC ACCGGACCCA GCTGTGAGAC AGACATTGAT 
GAGTGCTCTG AGGGCTTTGT TCAGTGTGAC AGCCGTGCCA ACTGCATTAA CCTGCCTGGG 
TGGTACCACT GTGAGTGCAG AGATGGCTAC CATGACAATG GGATGTTTGC ACCAGGTGGA 
GAATCCTGTG AAGATATTGA TGAATGTGGG ACTGGGAGGC ACAGCTGTGC CAATGACACC 
ATTTGCTTCA ACTTGGACGG TGGCTACGAT TGCCGGTGTC CCCATGGAAA GAACTGCACA 
GGGGACTGCG TGCACGACGG GAAAGTCAAA CACAACGGCC AGATCTGGGT GCTGGAGAAC 
GACAGGTGCT CTGTGTGTTC CTGCCAGACT GGATTTGTTA TGTGTCGACG GATGGTCTGT 
GACTGCGAAA ACCCCACAGT TGACCTCTCC TGCTGCCCTG AGTGCGACCC AAGGCTGAGC 
AGCCAGTGCC TGCATCAAAA CGGGGAAACC GTGTACAACA GCGGTGACAC CTGGGTCCAG 
GATTGCCGTC AGTGCCGCTG CTTGCAAGGA GAAGTTGACT GCTGGCCCCT GGCTTGCCCA 
GAGGTAGAGT GTGAATTTAG TGTCCTTCCT GAGAACGAGT GCTGCCCACG CTGTGTCACC 
GATCCTTGTC AGGCTGACAC CATCCGCAAT GACATCACCA AAACCTGCCT GGACGAGATG 
AACGTGGTTC GCTTCACTGG GTCTTCCTGG ATCAAGCACG GCACAGAGTG CACCCTCTGC 
CAGTGCAAGA ACGGCCACGT GTGCTGCTCA GTGGACCCAC AGTGCCTCCA GGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nell2 ( XM_008765797.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764019.1 Rattus norvegicus neural EGFL like 2 (Nell2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa07515
Clone ID Related Accession (Same CDS sequence)	XM_008765797.2 , NM_031070.2 , XM_008765798.2
Accession Version	XM_008765797.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C-binding protein NELL2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGCCA TGGAATCCCG GGTATTACTG AGAACGTTCT GCGTGATCCT CGGGCTCGAA 
GCGGTTTGGG GACTTGGTGT GGACCCCTCC CTACAGATTG ACGTCTTATC AGAGTTAGAA 
CTTGGGGAGT CCACAGCTGG AGTGCGCCAA GTCCCAGGAC TGCATAATGG GACGAAAGCC 
TTCCTCTTCC AAGATTCTCC CAGAAGCATA AAAGCACCCA TTGCTACAGC TGAGCGGTTT 
TTCCAGAAGC TGAGGAATAA ACACGAGTTC ACAATTCTGG TGACCCTGAA ACAGATCCAC 
TTAAATTCGG GAGTCATTCT CTCCATCCAC CACTTGGATC ACAGGTACCT GGAACTGGAA 
AGCAGCGGCC ACCGGAATGA GATCAGACTG CATTACCGCT CTGGAACTCA CCGCCCGCAC 
ACGGAAGTGT TTCCTTACAT TTTGGCTGAT GCCAAGTGGC ACAAGCTCTC CTTAGCCTTC 
AGTGCCTCCC ACTTAATTTT ACACATCGAC TGCAACAAGA TCTATGAACG AGTGGTGGAA 
ATGCCTTCTA CAGACTTGCC TCTGGGCACC ACATTTTGGT TGGGACAGAG AAATAACGCA 
CACGGGTATT TTAAGGGAAT AATGCAAGAT GTGCAATTAC TTGTCATGCC CCAGGGGTTC 
ATCGCTCAGT GCCCGGATCT TAATCGAACC TGTCCAACAT GCAACGACTT CCATGGGCTT 
GTGCAGAAAA TCATGGAGCT GCAGGACATT TTATCGAAGA CGTCAGCCAA GTTGTCTAGA 
GCTGAACAAC GAATGAACAG GCTGGATCAG TGCTACTGTG AGCGGACGTG CACCATGAAG 
GGAACCACCT ACCGGGAGTT CGAGTCCTGG ACAGACGGCT GCAAGAACTG CACATGCTTG 
AATGGGACCA TCCAGTGCGA GACTCTGGTC TGCCCTGCTC CCGACTGCCC GGCTAAATCG 
GCTCCAGCGT ACGTGGATGG CAAGTGCTGT AAGGAGTGCA AGTCCACCTG CCAGTTCCAG 
GGGCGGAGCT ACTTTGAGGG AGAAAGGAGC ACAGTCTTCT CAGCTTCCGG AATGTGCGTC 
TTGTATGAAT GCAAGGATCA GACCATGAAG CTTGTTGAGA ACGCCGGCTG CCCGGCTTTA 
GATTGCCCCG AGTCTCATCA GATCGCCTTG TCTCACAGCT GCTGCAAGGT TTGCAAAGGT 
TATGACTTCT GTTCTGAGAA GCATACATGC ATGGAGAACT CAGTCTGCAG GAACCTGAAC 
GACAGGGCAG TGTGCAGCTG CCGGGATGGT TTCCGGGCCC TCCGGGAGGA CAATGCCTAC 
TGTGAAGACA TTGACGAGTG TGCAGAGGGG CGCCATTACT GCCGTGAGAA CACCATGTGT 
GTGAACACAC CGGGCTCTTT CCTGTGTATC TGCCAAACAG GGTACATCAG AATCGACGAT 
TACTCGTGTA CGGAACATGA CGAGTGCCTC ACAAACCAGC ACAATTGTGA CGAGAACGCT 
TTGTGCTTTA ACACCGTTGG AGGTCACAAC TGCGTCTGCA AGCCTGGCTA CACTGGGAAT 
GGAACCACGT GCAAAGCTTT CTGCAAAGAC GGCTGCAGAA ACGGAGGTGC CTGCATTGCT 
GCCAATGTCT GTGCTTGCCC ACAAGGCTTC ACCGGACCCA GCTGTGAGAC AGACATTGAT 
GAGTGCTCTG AGGGCTTTGT TCAGTGTGAC AGCCGTGCCA ACTGCATTAA CCTGCCTGGG 
TGGTACCACT GTGAGTGCAG AGATGGCTAC CATGACAATG GGATGTTTGC ACCAGGTGGA 
GAATCCTGTG AAGATATTGA TGAATGTGGG ACTGGGAGGC ACAGCTGTGC CAATGACACC 
ATTTGCTTCA ACTTGGACGG TGGCTACGAT TGCCGGTGTC CCCATGGAAA GAACTGCACA 
GGGGACTGCG TGCACGACGG GAAAGTCAAA CACAACGGCC AGATCTGGGT GCTGGAGAAC 
GACAGGTGCT CTGTGTGTTC CTGCCAGACT GGATTTGTTA TGTGTCGACG GATGGTCTGT 
GACTGCGAAA ACCCCACAGT TGACCTCTCC TGCTGCCCTG AGTGCGACCC AAGGCTGAGC 
AGCCAGTGCC TGCATCAAAA CGGGGAAACC GTGTACAACA GCGGTGACAC CTGGGTCCAG 
GATTGCCGTC AGTGCCGCTG CTTGCAAGGA GAAGTTGACT GCTGGCCCCT GGCTTGCCCA 
GAGGTAGAGT GTGAATTTAG TGTCCTTCCT GAGAACGAGT GCTGCCCACG CTGTGTCACC 
GATCCTTGTC AGGCTGACAC CATCCGCAAT GACATCACCA AAACCTGCCT GGACGAGATG 
AACGTGGTTC GCTTCACTGG GTCTTCCTGG ATCAAGCACG GCACAGAGTG CACCCTCTGC 
CAGTGCAAGA ACGGCCACGT GTGCTGCTCA GTGGACCCAC AGTGCCTCCA GGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764019.1
CDS	426..2885
Translation	MHAMESRVLLRTFCVILGLEAVWGLGVDPSLQIDVLSELELGESTAGVRQVPGLHNGTKAFLFQDSPRSIKAPIATAERFFQKLRNKHEFTILVTLKQIHLNSGVILSIHHLDHRYLELESSGHRNEIRLHYRSGTHRPHTEVFPYILADAKWHKLSLAFSASHLILHIDCNKIYERVVEMPSTDLPLGTTFWLGQRNNAHGYFKGIMQDVQLLVMPQGFIAQCPDLNRTCPTCNDFHGLVQKIMELQDILSKTSAKLSRAEQRMNRLDQCYCERTCTMKGTTYREFESWTDGCKNCTCLNGTIQCETLVCPAPDCPAKSAPAYVDGKCCKECKSTCQFQGRSYFEGERSTVFSASGMCVLYECKDQTMKLVENAGCPALDCPESHQIALSHSCCKVCKGYDFCSEKHTCMENSVCRNLNDRAVCSCRDGFRALREDNAYCEDIDECAEGRHYCRENTMCVNTPGSFLCICQTGYIRIDDYSCTEHDECLTNQHNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSEGFVQCDSRANCINLPGWYHCECRDGYHDNGMFAPGGESCEDIDECGTGRHSCANDTICFNLDGGYDCRCPHGKNCTGDCVHDGKVKHNGQIWVLENDRCSVCSCQTGFVMCRRMVCDCENPTVDLSCCPECDPRLSSQCLHQNGETVYNSGDTWVQDCRQCRCLQGEVDCWPLACPEVECEFSVLPENECCPRCVTDPCQADTIRNDITKTCLDEMNVVRFTGSSWIKHGTECTLCQCKNGHVCCSVDPQCLQEL

Target ORF information:

RefSeq Version	XM_008765797.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neural EGFL like 2 (Nell2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765797.2

ORF Insert Sequence:

ATGCACGCCA TGGAATCCCG GGTATTACTG AGAACGTTCT GCGTGATCCT CGGGCTCGAA 
GCGGTTTGGG GACTTGGTGT GGACCCCTCC CTACAGATTG ACGTCTTATC AGAGTTAGAA 
CTTGGGGAGT CCACAGCTGG AGTGCGCCAA GTCCCAGGAC TGCATAATGG GACGAAAGCC 
TTCCTCTTCC AAGATTCTCC CAGAAGCATA AAAGCACCCA TTGCTACAGC TGAGCGGTTT 
TTCCAGAAGC TGAGGAATAA ACACGAGTTC ACAATTCTGG TGACCCTGAA ACAGATCCAC 
TTAAATTCGG GAGTCATTCT CTCCATCCAC CACTTGGATC ACAGGTACCT GGAACTGGAA 
AGCAGCGGCC ACCGGAATGA GATCAGACTG CATTACCGCT CTGGAACTCA CCGCCCGCAC 
ACGGAAGTGT TTCCTTACAT TTTGGCTGAT GCCAAGTGGC ACAAGCTCTC CTTAGCCTTC 
AGTGCCTCCC ACTTAATTTT ACACATCGAC TGCAACAAGA TCTATGAACG AGTGGTGGAA 
ATGCCTTCTA CAGACTTGCC TCTGGGCACC ACATTTTGGT TGGGACAGAG AAATAACGCA 
CACGGGTATT TTAAGGGAAT AATGCAAGAT GTGCAATTAC TTGTCATGCC CCAGGGGTTC 
ATCGCTCAGT GCCCGGATCT TAATCGAACC TGTCCAACAT GCAACGACTT CCATGGGCTT 
GTGCAGAAAA TCATGGAGCT GCAGGACATT TTATCGAAGA CGTCAGCCAA GTTGTCTAGA 
GCTGAACAAC GAATGAACAG GCTGGATCAG TGCTACTGTG AGCGGACGTG CACCATGAAG 
GGAACCACCT ACCGGGAGTT CGAGTCCTGG ACAGACGGCT GCAAGAACTG CACATGCTTG 
AATGGGACCA TCCAGTGCGA GACTCTGGTC TGCCCTGCTC CCGACTGCCC GGCTAAATCG 
GCTCCAGCGT ACGTGGATGG CAAGTGCTGT AAGGAGTGCA AGTCCACCTG CCAGTTCCAG 
GGGCGGAGCT ACTTTGAGGG AGAAAGGAGC ACAGTCTTCT CAGCTTCCGG AATGTGCGTC 
TTGTATGAAT GCAAGGATCA GACCATGAAG CTTGTTGAGA ACGCCGGCTG CCCGGCTTTA 
GATTGCCCCG AGTCTCATCA GATCGCCTTG TCTCACAGCT GCTGCAAGGT TTGCAAAGGT 
TATGACTTCT GTTCTGAGAA GCATACATGC ATGGAGAACT CAGTCTGCAG GAACCTGAAC 
GACAGGGCAG TGTGCAGCTG CCGGGATGGT TTCCGGGCCC TCCGGGAGGA CAATGCCTAC 
TGTGAAGACA TTGACGAGTG TGCAGAGGGG CGCCATTACT GCCGTGAGAA CACCATGTGT 
GTGAACACAC CGGGCTCTTT CCTGTGTATC TGCCAAACAG GGTACATCAG AATCGACGAT 
TACTCGTGTA CGGAACATGA CGAGTGCCTC ACAAACCAGC ACAATTGTGA CGAGAACGCT 
TTGTGCTTTA ACACCGTTGG AGGTCACAAC TGCGTCTGCA AGCCTGGCTA CACTGGGAAT 
GGAACCACGT GCAAAGCTTT CTGCAAAGAC GGCTGCAGAA ACGGAGGTGC CTGCATTGCT 
GCCAATGTCT GTGCTTGCCC ACAAGGCTTC ACCGGACCCA GCTGTGAGAC AGACATTGAT 
GAGTGCTCTG AGGGCTTTGT TCAGTGTGAC AGCCGTGCCA ACTGCATTAA CCTGCCTGGG 
TGGTACCACT GTGAGTGCAG AGATGGCTAC CATGACAATG GGATGTTTGC ACCAGGTGGA 
GAATCCTGTG AAGATATTGA TGAATGTGGG ACTGGGAGGC ACAGCTGTGC CAATGACACC 
ATTTGCTTCA ACTTGGACGG TGGCTACGAT TGCCGGTGTC CCCATGGAAA GAACTGCACA 
GGGGACTGCG TGCACGACGG GAAAGTCAAA CACAACGGCC AGATCTGGGT GCTGGAGAAC 
GACAGGTGCT CTGTGTGTTC CTGCCAGACT GGATTTGTTA TGTGTCGACG GATGGTCTGT 
GACTGCGAAA ACCCCACAGT TGACCTCTCC TGCTGCCCTG AGTGCGACCC AAGGCTGAGC 
AGCCAGTGCC TGCATCAAAA CGGGGAAACC GTGTACAACA GCGGTGACAC CTGGGTCCAG 
GATTGCCGTC AGTGCCGCTG CTTGCAAGGA GAAGTTGACT GCTGGCCCCT GGCTTGCCCA 
GAGGTAGAGT GTGAATTTAG TGTCCTTCCT GAGAACGAGT GCTGCCCACG CTGTGTCACC 
GATCCTTGTC AGGCTGACAC CATCCGCAAT GACATCACCA AAACCTGCCT GGACGAGATG 
AACGTGGTTC GCTTCACTGG GTCTTCCTGG ATCAAGCACG GCACAGAGTG CACCCTCTGC 
CAGTGCAAGA ACGGCCACGT GTGCTGCTCA GTGGACCCAC AGTGCCTCCA GGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nell2 ( XM_008765798.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764020.1 Rattus norvegicus neural EGFL like 2 (Nell2), transcript variant X2, mRNA.

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CloneID	ORa07515
Clone ID Related Accession (Same CDS sequence)	XM_008765797.2 , NM_031070.2 , XM_008765798.2
Accession Version	XM_008765798.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C-binding protein NELL2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765798.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGCCA TGGAATCCCG GGTATTACTG AGAACGTTCT GCGTGATCCT CGGGCTCGAA 
GCGGTTTGGG GACTTGGTGT GGACCCCTCC CTACAGATTG ACGTCTTATC AGAGTTAGAA 
CTTGGGGAGT CCACAGCTGG AGTGCGCCAA GTCCCAGGAC TGCATAATGG GACGAAAGCC 
TTCCTCTTCC AAGATTCTCC CAGAAGCATA AAAGCACCCA TTGCTACAGC TGAGCGGTTT 
TTCCAGAAGC TGAGGAATAA ACACGAGTTC ACAATTCTGG TGACCCTGAA ACAGATCCAC 
TTAAATTCGG GAGTCATTCT CTCCATCCAC CACTTGGATC ACAGGTACCT GGAACTGGAA 
AGCAGCGGCC ACCGGAATGA GATCAGACTG CATTACCGCT CTGGAACTCA CCGCCCGCAC 
ACGGAAGTGT TTCCTTACAT TTTGGCTGAT GCCAAGTGGC ACAAGCTCTC CTTAGCCTTC 
AGTGCCTCCC ACTTAATTTT ACACATCGAC TGCAACAAGA TCTATGAACG AGTGGTGGAA 
ATGCCTTCTA CAGACTTGCC TCTGGGCACC ACATTTTGGT TGGGACAGAG AAATAACGCA 
CACGGGTATT TTAAGGGAAT AATGCAAGAT GTGCAATTAC TTGTCATGCC CCAGGGGTTC 
ATCGCTCAGT GCCCGGATCT TAATCGAACC TGTCCAACAT GCAACGACTT CCATGGGCTT 
GTGCAGAAAA TCATGGAGCT GCAGGACATT TTATCGAAGA CGTCAGCCAA GTTGTCTAGA 
GCTGAACAAC GAATGAACAG GCTGGATCAG TGCTACTGTG AGCGGACGTG CACCATGAAG 
GGAACCACCT ACCGGGAGTT CGAGTCCTGG ACAGACGGCT GCAAGAACTG CACATGCTTG 
AATGGGACCA TCCAGTGCGA GACTCTGGTC TGCCCTGCTC CCGACTGCCC GGCTAAATCG 
GCTCCAGCGT ACGTGGATGG CAAGTGCTGT AAGGAGTGCA AGTCCACCTG CCAGTTCCAG 
GGGCGGAGCT ACTTTGAGGG AGAAAGGAGC ACAGTCTTCT CAGCTTCCGG AATGTGCGTC 
TTGTATGAAT GCAAGGATCA GACCATGAAG CTTGTTGAGA ACGCCGGCTG CCCGGCTTTA 
GATTGCCCCG AGTCTCATCA GATCGCCTTG TCTCACAGCT GCTGCAAGGT TTGCAAAGGT 
TATGACTTCT GTTCTGAGAA GCATACATGC ATGGAGAACT CAGTCTGCAG GAACCTGAAC 
GACAGGGCAG TGTGCAGCTG CCGGGATGGT TTCCGGGCCC TCCGGGAGGA CAATGCCTAC 
TGTGAAGACA TTGACGAGTG TGCAGAGGGG CGCCATTACT GCCGTGAGAA CACCATGTGT 
GTGAACACAC CGGGCTCTTT CCTGTGTATC TGCCAAACAG GGTACATCAG AATCGACGAT 
TACTCGTGTA CGGAACATGA CGAGTGCCTC ACAAACCAGC ACAATTGTGA CGAGAACGCT 
TTGTGCTTTA ACACCGTTGG AGGTCACAAC TGCGTCTGCA AGCCTGGCTA CACTGGGAAT 
GGAACCACGT GCAAAGCTTT CTGCAAAGAC GGCTGCAGAA ACGGAGGTGC CTGCATTGCT 
GCCAATGTCT GTGCTTGCCC ACAAGGCTTC ACCGGACCCA GCTGTGAGAC AGACATTGAT 
GAGTGCTCTG AGGGCTTTGT TCAGTGTGAC AGCCGTGCCA ACTGCATTAA CCTGCCTGGG 
TGGTACCACT GTGAGTGCAG AGATGGCTAC CATGACAATG GGATGTTTGC ACCAGGTGGA 
GAATCCTGTG AAGATATTGA TGAATGTGGG ACTGGGAGGC ACAGCTGTGC CAATGACACC 
ATTTGCTTCA ACTTGGACGG TGGCTACGAT TGCCGGTGTC CCCATGGAAA GAACTGCACA 
GGGGACTGCG TGCACGACGG GAAAGTCAAA CACAACGGCC AGATCTGGGT GCTGGAGAAC 
GACAGGTGCT CTGTGTGTTC CTGCCAGACT GGATTTGTTA TGTGTCGACG GATGGTCTGT 
GACTGCGAAA ACCCCACAGT TGACCTCTCC TGCTGCCCTG AGTGCGACCC AAGGCTGAGC 
AGCCAGTGCC TGCATCAAAA CGGGGAAACC GTGTACAACA GCGGTGACAC CTGGGTCCAG 
GATTGCCGTC AGTGCCGCTG CTTGCAAGGA GAAGTTGACT GCTGGCCCCT GGCTTGCCCA 
GAGGTAGAGT GTGAATTTAG TGTCCTTCCT GAGAACGAGT GCTGCCCACG CTGTGTCACC 
GATCCTTGTC AGGCTGACAC CATCCGCAAT GACATCACCA AAACCTGCCT GGACGAGATG 
AACGTGGTTC GCTTCACTGG GTCTTCCTGG ATCAAGCACG GCACAGAGTG CACCCTCTGC 
CAGTGCAAGA ACGGCCACGT GTGCTGCTCA GTGGACCCAC AGTGCCTCCA GGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764020.1
CDS	42..2501
Translation	MHAMESRVLLRTFCVILGLEAVWGLGVDPSLQIDVLSELELGESTAGVRQVPGLHNGTKAFLFQDSPRSIKAPIATAERFFQKLRNKHEFTILVTLKQIHLNSGVILSIHHLDHRYLELESSGHRNEIRLHYRSGTHRPHTEVFPYILADAKWHKLSLAFSASHLILHIDCNKIYERVVEMPSTDLPLGTTFWLGQRNNAHGYFKGIMQDVQLLVMPQGFIAQCPDLNRTCPTCNDFHGLVQKIMELQDILSKTSAKLSRAEQRMNRLDQCYCERTCTMKGTTYREFESWTDGCKNCTCLNGTIQCETLVCPAPDCPAKSAPAYVDGKCCKECKSTCQFQGRSYFEGERSTVFSASGMCVLYECKDQTMKLVENAGCPALDCPESHQIALSHSCCKVCKGYDFCSEKHTCMENSVCRNLNDRAVCSCRDGFRALREDNAYCEDIDECAEGRHYCRENTMCVNTPGSFLCICQTGYIRIDDYSCTEHDECLTNQHNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSEGFVQCDSRANCINLPGWYHCECRDGYHDNGMFAPGGESCEDIDECGTGRHSCANDTICFNLDGGYDCRCPHGKNCTGDCVHDGKVKHNGQIWVLENDRCSVCSCQTGFVMCRRMVCDCENPTVDLSCCPECDPRLSSQCLHQNGETVYNSGDTWVQDCRQCRCLQGEVDCWPLACPEVECEFSVLPENECCPRCVTDPCQADTIRNDITKTCLDEMNVVRFTGSSWIKHGTECTLCQCKNGHVCCSVDPQCLQEL

Target ORF information:

RefSeq Version	XM_008765798.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neural EGFL like 2 (Nell2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765798.2

ORF Insert Sequence:

ATGCACGCCA TGGAATCCCG GGTATTACTG AGAACGTTCT GCGTGATCCT CGGGCTCGAA 
GCGGTTTGGG GACTTGGTGT GGACCCCTCC CTACAGATTG ACGTCTTATC AGAGTTAGAA 
CTTGGGGAGT CCACAGCTGG AGTGCGCCAA GTCCCAGGAC TGCATAATGG GACGAAAGCC 
TTCCTCTTCC AAGATTCTCC CAGAAGCATA AAAGCACCCA TTGCTACAGC TGAGCGGTTT 
TTCCAGAAGC TGAGGAATAA ACACGAGTTC ACAATTCTGG TGACCCTGAA ACAGATCCAC 
TTAAATTCGG GAGTCATTCT CTCCATCCAC CACTTGGATC ACAGGTACCT GGAACTGGAA 
AGCAGCGGCC ACCGGAATGA GATCAGACTG CATTACCGCT CTGGAACTCA CCGCCCGCAC 
ACGGAAGTGT TTCCTTACAT TTTGGCTGAT GCCAAGTGGC ACAAGCTCTC CTTAGCCTTC 
AGTGCCTCCC ACTTAATTTT ACACATCGAC TGCAACAAGA TCTATGAACG AGTGGTGGAA 
ATGCCTTCTA CAGACTTGCC TCTGGGCACC ACATTTTGGT TGGGACAGAG AAATAACGCA 
CACGGGTATT TTAAGGGAAT AATGCAAGAT GTGCAATTAC TTGTCATGCC CCAGGGGTTC 
ATCGCTCAGT GCCCGGATCT TAATCGAACC TGTCCAACAT GCAACGACTT CCATGGGCTT 
GTGCAGAAAA TCATGGAGCT GCAGGACATT TTATCGAAGA CGTCAGCCAA GTTGTCTAGA 
GCTGAACAAC GAATGAACAG GCTGGATCAG TGCTACTGTG AGCGGACGTG CACCATGAAG 
GGAACCACCT ACCGGGAGTT CGAGTCCTGG ACAGACGGCT GCAAGAACTG CACATGCTTG 
AATGGGACCA TCCAGTGCGA GACTCTGGTC TGCCCTGCTC CCGACTGCCC GGCTAAATCG 
GCTCCAGCGT ACGTGGATGG CAAGTGCTGT AAGGAGTGCA AGTCCACCTG CCAGTTCCAG 
GGGCGGAGCT ACTTTGAGGG AGAAAGGAGC ACAGTCTTCT CAGCTTCCGG AATGTGCGTC 
TTGTATGAAT GCAAGGATCA GACCATGAAG CTTGTTGAGA ACGCCGGCTG CCCGGCTTTA 
GATTGCCCCG AGTCTCATCA GATCGCCTTG TCTCACAGCT GCTGCAAGGT TTGCAAAGGT 
TATGACTTCT GTTCTGAGAA GCATACATGC ATGGAGAACT CAGTCTGCAG GAACCTGAAC 
GACAGGGCAG TGTGCAGCTG CCGGGATGGT TTCCGGGCCC TCCGGGAGGA CAATGCCTAC 
TGTGAAGACA TTGACGAGTG TGCAGAGGGG CGCCATTACT GCCGTGAGAA CACCATGTGT 
GTGAACACAC CGGGCTCTTT CCTGTGTATC TGCCAAACAG GGTACATCAG AATCGACGAT 
TACTCGTGTA CGGAACATGA CGAGTGCCTC ACAAACCAGC ACAATTGTGA CGAGAACGCT 
TTGTGCTTTA ACACCGTTGG AGGTCACAAC TGCGTCTGCA AGCCTGGCTA CACTGGGAAT 
GGAACCACGT GCAAAGCTTT CTGCAAAGAC GGCTGCAGAA ACGGAGGTGC CTGCATTGCT 
GCCAATGTCT GTGCTTGCCC ACAAGGCTTC ACCGGACCCA GCTGTGAGAC AGACATTGAT 
GAGTGCTCTG AGGGCTTTGT TCAGTGTGAC AGCCGTGCCA ACTGCATTAA CCTGCCTGGG 
TGGTACCACT GTGAGTGCAG AGATGGCTAC CATGACAATG GGATGTTTGC ACCAGGTGGA 
GAATCCTGTG AAGATATTGA TGAATGTGGG ACTGGGAGGC ACAGCTGTGC CAATGACACC 
ATTTGCTTCA ACTTGGACGG TGGCTACGAT TGCCGGTGTC CCCATGGAAA GAACTGCACA 
GGGGACTGCG TGCACGACGG GAAAGTCAAA CACAACGGCC AGATCTGGGT GCTGGAGAAC 
GACAGGTGCT CTGTGTGTTC CTGCCAGACT GGATTTGTTA TGTGTCGACG GATGGTCTGT 
GACTGCGAAA ACCCCACAGT TGACCTCTCC TGCTGCCCTG AGTGCGACCC AAGGCTGAGC 
AGCCAGTGCC TGCATCAAAA CGGGGAAACC GTGTACAACA GCGGTGACAC CTGGGTCCAG 
GATTGCCGTC AGTGCCGCTG CTTGCAAGGA GAAGTTGACT GCTGGCCCCT GGCTTGCCCA 
GAGGTAGAGT GTGAATTTAG TGTCCTTCCT GAGAACGAGT GCTGCCCACG CTGTGTCACC 
GATCCTTGTC AGGCTGACAC CATCCGCAAT GACATCACCA AAACCTGCCT GGACGAGATG 
AACGTGGTTC GCTTCACTGG GTCTTCCTGG ATCAAGCACG GCACAGAGTG CACCCTCTGC 
CAGTGCAAGA ACGGCCACGT GTGCTGCTCA GTGGACCCAC AGTGCCTCCA GGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats.
Molecules and cells40(3)186-194(2017 Mar)
Jeong JK,Kim JG,Kim HR,Lee TH,Park JW,Lee BJ

The molecular mechanism of NELL2 movement and secretion in hippocampal progenitor HiB5 cells.
Molecules and cells36(6)527-33(2013 Dec)
Ha CM,Hwang EM,Kim E,Lee DY,Chang S,Lee BJ,Hong SG,Park JY

The neuronal EGF-related gene Nell2 interacts with Macf1 and supports survival of retinal ganglion cells after optic nerve injury.
PloS one7(4)e34810(2012)
Munemasa Y,Chang CS,Kwong JM,Kyung H,Kitaoka Y,Caprioli J,Piri N

Regulation of the female rat estrous cycle by a neural cell-specific epidermal growth factor-like repeat domain containing protein, NELL2.
Molecules and cells32(2)203-7(2011 Aug)
Ryu BJ,Kim HR,Jeong JK,Lee BJ

A neuron-specific EGF family protein, NELL2, promotes survival of neurons through mitogen-activated protein kinases.
Brain research. Molecular brain research116(1-2)86-93(2003 Aug)
Aihara K,Kuroda S,Kanayama N,Matsuyama S,Tanizawa K,Horie M

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Nell2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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