Mvk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mvk
Entrez Gene ID	81727
Full Name	mevalonate kinase
Synonyms	Lrbp
General protein information	Preferred Names mevalonate kinase Names mevalonate kinase LHR mRNA binding protein MK
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16
Summary	kinase that catalyzes the ATP-dependent phosphorylation of mevalonic acid to form mevalonate 5-phosphate; involved in the cholesterol biosynthetic pathway [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031063.1	NP_112325.1	mevalonate kinase

Pathways from BioSystems

Homologies

	MVK	XP_001105815.1
Canis lupus familiaris (dog)	MVK	XP_005636378.1
Schizosaccharomyces pombe (fission yeast)	erg12	NP_592837.1
Homo sapiens (human)	MVK	NP_001107657.1
Pan troglodytes (chimpanzee)	MVK	XP_003313983.1
Magnaporthe oryzae (rice blast fungus)	MGG_16219	XP_003709688.1
Xenopus tropicalis (tropical clawed frog)	mvk	XP_002932110.2
Gallus gallus (chicken)	MVK	XP_001231562.3
Danio rerio (zebrafish)	mvk	NP_001007350.1
Neurospora crassa	NCU03633	XP_961360.1
Bos taurus (cattle)	MVK	NP_001015528.1
Mus musculus (house mouse)	Mvk	NP_076045.1
Rattus norvegicus (Norway rat)	Mvk	NP_112325.1
Kluyveromyces lactis	KLLA0C07469g	XP_452532.1
Eremothecium gossypii	AGOS_AER335W	NP_985191.1
Gallus gallus (chicken)	LOC100857437	XP_003643684.2
Caenorhabditis elegans (roundworm)	mvk-1	NP_001022869.1
Saccharomyces cerevisiae (baker's yeast)	ERG12	NP_013935.1
Arabidopsis thaliana (thale cress)	MK	NP_198097.1
Oryza sativa (rice)	Os10g0329300	NP_001064360.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Regulation of LH receptor mRNA binding protein by miR-122 in rat ovaries.
Menon B, Sinden J, Franzo-Romain M, Botta RB, Menon KM
Endocrinology154(12)4826-34(2013 Dec)

Association of luteinizing hormone receptor (LHR) mRNA with its binding protein leads to decapping and degradation of the mRNA in the p bodies.
Menon B, Sinden J, Menon KM
Biochimica et biophysica acta1833(5)1173-9(2013 May)

Identification and characterization of proteins that selectively interact with the LHR mRNA binding protein (LRBP) in rat ovaries.
Wang L, Gulappa T, Menon KM
Biochimica et biophysica acta1803(5)591-7(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that hCG-induced down-regulation of LHR mRNA expression is mediated by activation of cAMP/PKA/ERK pathways to increase miR-122 expression, which then increases LRBP expression through the activation of SREBPs.
Title: Regulation of LH receptor mRNA binding protein by miR-122 in rat ovaries.

These data suggest that the association of LRBP with luteinizing hormone receptor mRNA results in the translocation of the messenger ribonucleoprotein complex to the p bodies leading to decapping and degradation.
Title: Association of luteinizing hormone receptor (LHR) mRNA with its binding protein leads to decapping and degradation of the mRNA in the p bodies.

during ligand-induced downregulation, LRBP translocates to ribosomes and associates with LHR mRNA to form an untranslatable ribonucleoprotein complex and inhibits LHR mRNA translation, paving the way to its degradation.
Title: Evidence for the association of luteinizing hormone receptor mRNA-binding protein with the translating ribosomes during receptor downregulation.

Comparison of animal MK protein alignments and structures with those of a MK protein from Streptococcus pneumoniae indicates that sequence differences between N- and C-terminal domains correlate with differences in interdomain angles.
Title: Biochemical and structural basis for feedback inhibition of mevalonate kinase and isoprenoid metabolism.

Rat mevalonate kinase A141C variant enzyme has a new disulfide bond, which makes the variant protein enhance its thermal activity and resist to urea denaturation.
Title: Effect of a disulfide bond on mevalonate kinase.

The following Mvk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mvk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07497	NM_031063.1 Latest version!	Rattus norvegicus mevalonate kinase (Mvk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mvk ( NM_031063.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112325.1 Rattus norvegicus mevalonate kinase (Mvk), mRNA.

Price & Availability↑

CloneID	ORa07497
Clone ID Related Accession (Same CDS sequence)	NM_031063.1
Accession Version	NM_031063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	mevalonate kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M29472.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M29472.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTTGTCAG AAGTCCTGCT GGTGTCTGCT CCAGGGAAAG TCATTCTCCA TGGAGAACAT 
GCTGTGGTCC ATGGCAAGGT AGCTCTGGCG GTGGCCTTGA ACTTGAGAAC ATTTCTCGTG 
CTGCGACCGC AGAGCAATGG GAAAGTGAGC CTCAATTTAC CAAACGTCGG TATTAAGCAG 
GTCTGGGATG TGGCCACACT TCAGCTGCTG GACACAGGCT TTCTTGAGCA AGGCGATGTC 
CCGGCACCCA CCTTGGAGCA ACTGGAGAAG CTGAAGAAGG TGGCGGGCCT CCCCCGAGAC 
TGTGTAGGCA ACGAAGGCCT GTCTCTGCTT GCCTTTCTGT ACCTGTACCT GGCTATCTGC 
CGGAAACAGA GGACACTCCC AAGCCTGGAC ATCATGGTGT GGTCGGAACT GCCCCCTGGG 
GCGGGCTTGG GCTCCAGTGC AGCCTACTCG GTGTGTGTGG CAGCCGCCCT CCTGACTGCC 
TGTGAGGAGG TCACCAACCC GCTCAAGGAC AGGGGCTCCA TTGGCAGTTG GCCCGAGGAG 
GACCTGAAGT CAATTAACAA GTGGGCCTAC GAGGGGGAGA GAGTGATCCA TGGGAACCCC 
TCTGGCGTGG ACAATTCCGT CAGCACCTGG GGAGGAGCCC TGCGCTACCA GCAAGGGAAG 
ATGTCATCCT TGAAGAGGCT CCCAGCTCTG CAGATCCTGC TCACCAACAC CAAGGTCCCA 
CGAAGCACCA AGGCCCTCGT GGCTGGCGTC AGAAGCAGGC TAATCAAGTT CCCTGAGATC 
ATGGCCCCGC TCCTGACATC AATTGACGCA ATCTCCCTGG AGTGTGAGCG CGTGCTGGGA 
GAGATGGCGG CCGCACCAGT CCCAGAACAG TACCTTGTCC TAGAAGAGCT AATGGACATG 
AACCAGCACC ATCTGAATGC CCTTGGTGTG GGCCACGCCT CCCTGGACCA GCTCTGTCAG 
GTAACAGCAG CACATGGACT GCACAGCAAG CTGACTGGCG CAGGCGGCGG CGGCTGTGGC 
ATCACCCTCC TGAAGCCAGG TCTAGAGCGA GCAAAAGTGG AGGCCGCCAA GCAGGCCCTG 
ACCGGCTGCG GGTTTGACTG CTGGGAGACC AGCATTGGAG CGCCTGGGGT CTCCATGCAC 
TCAGCCACCT CCATAGAGGA CCCTGTCCGA CAAGCCCTGG GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112325.1
CDS	82..1269
Translation	MLSEVLLVSAPGKVILHGEHAVVHGKVALAVALNLRTFLVLRPQSNGKVSLNLPNVGIKQVWDVATLQLLDTGFLEQGDVPAPTLEQLEKLKKVAGLPRDCVGNEGLSLLAFLYLYLAICRKQRTLPSLDIMVWSELPPGAGLGSSAAYSVCVAAALLTACEEVTNPLKDRGSIGSWPEEDLKSINKWAYEGERVIHGNPSGVDNSVSTWGGALRYQQGKMSSLKRLPALQILLTNTKVPRSTKALVAGVRSRLIKFPEIMAPLLTSIDAISLECERVLGEMAAAPVPEQYLVLEELMDMNQHHLNALGVGHASLDQLCQVTAAHGLHSKLTGAGGGGCGITLLKPGLERAKVEAAKQALTGCGFDCWETSIGAPGVSMHSATSIEDPVRQALGL

Target ORF information:

RefSeq Version	NM_031063.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mevalonate kinase (Mvk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031063.1

ORF Insert Sequence:

ATGTTGTCAG AAGTCCTGCT GGTGTCTGCT CCAGGGAAAG TCATTCTCCA TGGAGAACAT 
GCTGTGGTCC ATGGCAAGGT AGCTCTGGCG GTGGCCTTGA ACTTGAGAAC ATTTCTCGTG 
CTGCGACCGC AGAGCAATGG GAAAGTGAGC CTCAATTTAC CAAACGTCGG TATTAAGCAG 
GTCTGGGATG TGGCCACACT TCAGCTGCTG GACACAGGCT TTCTTGAGCA AGGCGATGTC 
CCGGCACCCA CCTTGGAGCA ACTGGAGAAG CTGAAGAAGG TGGCGGGCCT CCCCCGAGAC 
TGTGTAGGCA ACGAAGGCCT GTCTCTGCTT GCCTTTCTGT ACCTGTACCT GGCTATCTGC 
CGGAAACAGA GGACACTCCC AAGCCTGGAC ATCATGGTGT GGTCGGAACT GCCCCCTGGG 
GCGGGCTTGG GCTCCAGTGC AGCCTACTCG GTGTGTGTGG CAGCCGCCCT CCTGACTGCC 
TGTGAGGAGG TCACCAACCC GCTCAAGGAC AGGGGCTCCA TTGGCAGTTG GCCCGAGGAG 
GACCTGAAGT CAATTAACAA GTGGGCCTAC GAGGGGGAGA GAGTGATCCA TGGGAACCCC 
TCTGGCGTGG ACAATTCCGT CAGCACCTGG GGAGGAGCCC TGCGCTACCA GCAAGGGAAG 
ATGTCATCCT TGAAGAGGCT CCCAGCTCTG CAGATCCTGC TCACCAACAC CAAGGTCCCA 
CGAAGCACCA AGGCCCTCGT GGCTGGCGTC AGAAGCAGGC TAATCAAGTT CCCTGAGATC 
ATGGCCCCGC TCCTGACATC AATTGACGCA ATCTCCCTGG AGTGTGAGCG CGTGCTGGGA 
GAGATGGCGG CCGCACCAGT CCCAGAACAG TACCTTGTCC TAGAAGAGCT AATGGACATG 
AACCAGCACC ATCTGAATGC CCTTGGTGTG GGCCACGCCT CCCTGGACCA GCTCTGTCAG 
GTAACAGCAG CACATGGACT GCACAGCAAG CTGACTGGCG CAGGCGGCGG CGGCTGTGGC 
ATCACCCTCC TGAAGCCAGG TCTAGAGCGA GCAAAAGTGG AGGCCGCCAA GCAGGCCCTG 
ACCGGCTGCG GGTTTGACTG CTGGGAGACC AGCATTGGAG CGCCTGGGGT CTCCATGCAC 
TCAGCCACCT CCATAGAGGA CCCTGTCCGA CAAGCCCTGG GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Regulation of LH receptor mRNA binding protein by miR-122 in rat ovaries.
Endocrinology154(12)4826-34(2013 Dec)
Menon B,Sinden J,Franzo-Romain M,Botta RB,Menon KM

Association of luteinizing hormone receptor (LHR) mRNA with its binding protein leads to decapping and degradation of the mRNA in the p bodies.
Biochimica et biophysica acta1833(5)1173-9(2013 May)
Menon B,Sinden J,Menon KM

Identification and characterization of proteins that selectively interact with the LHR mRNA binding protein (LRBP) in rat ovaries.
Biochimica et biophysica acta1803(5)591-7(2010 May)
Wang L,Gulappa T,Menon KM

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Mvk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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