Mipep cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mipep
Entrez Gene ID	81684
Full Name	mitochondrial intermediate peptidase
General protein information	Preferred Names mitochondrial intermediate peptidase Names mitochondrial intermediate peptidase MIP
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p12
Summary	enzyme that catalyzes the removal of amino-terminal octapeptides from mitochondrial proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008770780.2	XP_008769002.2	mitochondrial intermediate peptidase isoform X2
XM_008770779.2	XP_008769001.2	mitochondrial intermediate peptidase isoform X1
NM_031052.2	NP_112314.2	mitochondrial intermediate peptidase precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MIPEP	XP_003954496.1
Danio rerio (zebrafish)	mipep	XP_001344847.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007728	XP_308148.3
Schizosaccharomyces pombe (fission yeast)	oct1	NP_593013.1
Homo sapiens (human)	MIPEP	NP_005923.2
	MIPEP	XP_001090198.1
Canis lupus familiaris (dog)	MIPEP	XP_005635601.1
Rattus norvegicus (Norway rat)	Mipep	NP_112314.1
Drosophila melanogaster (fruit fly)	CG7791	NP_610183.1
Caenorhabditis elegans (roundworm)	CELE_Y67H2A.7	NP_502558.2
Kluyveromyces lactis	KLLA0B12397g	XP_452082.1
Gallus gallus (chicken)	MIPEP	XP_417136.2
Saccharomyces cerevisiae (baker's yeast)	OCT1	NP_012788.2
Eremothecium gossypii	AGOS_AFR198W	NP_985745.1
Oryza sativa (rice)	Os06g0686500	NP_001058401.1
Xenopus tropicalis (tropical clawed frog)	mipep	XP_004912119.1
Mus musculus (house mouse)	Mipep	NP_081712.2
Neurospora crassa	NCU02063	XP_964019.1
Bos taurus (cattle)	MIPEP	XP_002691892.1
Magnaporthe oryzae (rice blast fungus)	MGG_00487	XP_003718575.1
Arabidopsis thaliana (thale cress)	AT5G51540	NP_199967.2

Gene Ontology
Bibliography

Related articles in PubMed

Processing of mitochondrial presequences.
Mossmann D, Meisinger C, V?gtle FN
Biochimica et biophysica acta1819(9-10)1098-106(2012 Sep-Oct)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Sequence analysis of rat mitochondrial intermediate peptidase: similarity to zinc metallopeptidases and to a putative yeast homologue.
Isaya G, Kalousek F, Rosenberg LE
Proceedings of the National Academy of Sciences of the United States of America89(17)8317-21(1992 Sep)

Rat liver mitochondrial intermediate peptidase (MIP): purification and initial characterization.
Kalousek F, Isaya G, Rosenberg LE
The EMBO journal11(8)2803-9(1992 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mipep gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mipep cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa43720	NM_031052.2 Latest version!	Rattus norvegicus mitochondrial intermediate peptidase (Mipep), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00
ORa58653	XM_008770779.2 Latest version!	Rattus norvegicus mitochondrial intermediate peptidase (Mipep), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa58654	XM_008770780.2 Latest version!	Rattus norvegicus mitochondrial intermediate peptidase (Mipep), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mipep ( NM_031052.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112314.2 Rattus norvegicus mitochondrial intermediate peptidase (Mipep), mRNA.

Price & Availability↑

CloneID	ORa43720
Clone ID Related Accession (Same CDS sequence)	NM_031052.2
Accession Version	NM_031052.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial intermediate peptidase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07018119.1, FM065705.1, FN806671.1, FM117087.1, AABR07018120.1, FM100741.1, FM061883.1 and CR459947.1. On May 5, 2015 this sequence version replaced NM_031052.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: M96633.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGG CAGCCGGGAC GCGGTATGCC TACCGGCTGT GCGGCCGGCG AGCCGCGGCG 
GCTCTGCAGG GACACGCAGG CCGGAGCTGC GCCCGGAGCG TTAGCACTAG CTGGTCCCCG 
GTGGGCGCTG CCTTTAACGT CAAGCCCCAG GGCCACCTGT GGGACCTGTT GGGAGAGCGA 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAGGAG 
GAAGCCTTGA GGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC TCCAGGGCCA 
CAGACAGTCC TCATTTTCGA TGAGCTCTCG GATTGCCTCT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGAGCAG GCGTTCAGAG AGGCTGCGCA GGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACGAATG TGGAATTGTA TCAGAGTCTG 
CAGAAATTAC TAGATGACAA GAAGCTTATG GATTCCCTTG ATGCAGAAAC CAGGCGAGTA 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCCG TGGACCTCAA TGTTAAGATC TTGGACTTGA GTAGTGCTTT CCTCATGGGA 
ACCAACTTTC CCATCAAGAT CCAGAAGCAT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCTCGTGATG GAAGGCATTT AGTCATCGAT GGTCTACATG CGGAAGCCTC CGATGACCTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CTGATCAGTT GAAGTGTTTG 
GAGGAGTTGC TAAGCAGCCG AGATCTTCTG GCAAACCTAG TAGGCTATTC TACCTTTTCC 
CACAGGGCCC TCCAGGGAAC CATAGCCCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTG CGAAAGAACT AGAAAAGATT TTGAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTGAA CTAATGCCTT GGGACCCTCC CTACTACAGT 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATATTGTCC ATTCTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC CTATTTAACA GACTGTTGGG CGTCACCTTA 
TATGCAGAAC AGCCCTTCAA AGGAGAGGTG TGGTGTATTG ATGTCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGTT GCTGGGCTAC ATTTACTGTG ATTTTTTCCA GCGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGGGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTCC CAGTTGTGGT TCTCATGCTG AATCTCCCTC ATGCTTCCAG GGATTTCCCT 
ACCTTATTAA CTCCTGGGAT GATGGAAAAT CTCTTCCATG AAATGGGGCA TGCCATGCAC 
TCAATGCTCG GACGAACTCG GTACCAGCAT GTCACTGGGA CCAGGTGCCC TACTGACTTT 
GCTGAAGTTC CGTCTATTCT GATGGAGTAT TTTTCAAATG ATTACCGAGT AGTCAGCCAG 
TTTGCCAAGC ATTATCAGAC TGGTCAGCCA CTGCCGAAAG CTATGGTGTC TCGTCTCTGT 
GAATCTAAGA AGGTCTGTGC CGCCGCAGAA ATGCAGCTGC AGGTCTTTTA TGCCGCCCTG 
GATCAAATCT ACCATGGCCA GCATCCCCTG AAGAAGTCAA CCACAGACAT CCTCATGGAA 
ACACAAGAGC AGTTTTATGG CTTACCCTAT GTTCCTGACA CTGCCTGGCA GCTGAGGTTC 
AGCCACCTGG TGGGATATGG CGCCAAGTAT TACTCATACC TCATGTCCAG GGCTGTGGCA 
TCCATGGTCT GGAAGGAGTG CTTCCTACAG GATCCCTTTA ACAGGGCCGC CGGGGAGCGC 
TACCGCAGAG AGATGTTAGC CCACGGTGGG GGCAAGGAAC CTATGCTCAT GATTCAAGGC 
ATGCTGCAGA AATGTCCTTC CATCGATGAT TTTGTGGATG CCCTTGTGTC TGACTTGAAT 
CTGGACTTTG AAACTTTCTT CATGGATTCT AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112314.2
CDS	105..2240
Translation	MLLAAGTRYAYRLCGRRAAAALQGHAGRSCARSVSTSWSPVGAAFNVKPQGHLWDLLGERRGLFGVPELSTPEGFQVAQEEALRKTEWLVERACSTPPGPQTVLIFDELSDCLCRVADLADFVKIGHPEQAFREAAQEACRSIGTMVEKLNTNVELYQSLQKLLDDKKLMDSLDAETRRVAELFMFDFEISGIHLDEEKRRRAVDLNVKILDLSSAFLMGTNFPIKIQKHLLPEHIQHHFARDGRHLVIDGLHAEASDDLVREAAYKIFLYPNADQLKCLEELLSSRDLLANLVGYSTFSHRALQGTIAQTPETVMQFLEKLSEKLCERTRKDFEMMQGMKTKLNPQNSELMPWDPPYYSGVIRAERYNIEPSLYCPFLSLGACMEGLNVLFNRLLGVTLYAEQPFKGEVWCIDVRKLAVVHESEGLLGYIYCDFFQRANKPQQDCHFTIRGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTPGMMENLFHEMGHAMHSMLGRTRYQHVTGTRCPTDFAEVPSILMEYFSNDYRVVSQFAKHYQTGQPLPKAMVSRLCESKKVCAAAEMQLQVFYAALDQIYHGQHPLKKSTTDILMETQEQFYGLPYVPDTAWQLRFSHLVGYGAKYYSYLMSRAVASMVWKECFLQDPFNRAAGERYRREMLAHGGGKEPMLMIQGMLQKCPSIDDFVDALVSDLNLDFETFFMDSK

Target ORF information:

RefSeq Version	NM_031052.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitochondrial intermediate peptidase (Mipep), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031052.2

ORF Insert Sequence:

ATGCTGCTGG CAGCCGGGAC GCGGTATGCC TACCGGCTGT GCGGCCGGCG AGCCGCGGCG 
GCTCTGCAGG GACACGCAGG CCGGAGCTGC GCCCGGAGCG TTAGCACTAG CTGGTCCCCG 
GTGGGCGCTG CCTTTAACGT CAAGCCCCAG GGCCACCTGT GGGACCTGTT GGGAGAGCGA 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAGGAG 
GAAGCCTTGA GGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC TCCAGGGCCA 
CAGACAGTCC TCATTTTCGA TGAGCTCTCG GATTGCCTCT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGAGCAG GCGTTCAGAG AGGCTGCGCA GGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACGAATG TGGAATTGTA TCAGAGTCTG 
CAGAAATTAC TAGATGACAA GAAGCTTATG GATTCCCTTG ATGCAGAAAC CAGGCGAGTA 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCCG TGGACCTCAA TGTTAAGATC TTGGACTTGA GTAGTGCTTT CCTCATGGGA 
ACCAACTTTC CCATCAAGAT CCAGAAGCAT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCTCGTGATG GAAGGCATTT AGTCATCGAT GGTCTACATG CGGAAGCCTC CGATGACCTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CTGATCAGTT GAAGTGTTTG 
GAGGAGTTGC TAAGCAGCCG AGATCTTCTG GCAAACCTAG TAGGCTATTC TACCTTTTCC 
CACAGGGCCC TCCAGGGAAC CATAGCCCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTG CGAAAGAACT AGAAAAGATT TTGAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTGAA CTAATGCCTT GGGACCCTCC CTACTACAGT 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATATTGTCC ATTCTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC CTATTTAACA GACTGTTGGG CGTCACCTTA 
TATGCAGAAC AGCCCTTCAA AGGAGAGGTG TGGTGTATTG ATGTCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGTT GCTGGGCTAC ATTTACTGTG ATTTTTTCCA GCGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGGGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTCC CAGTTGTGGT TCTCATGCTG AATCTCCCTC ATGCTTCCAG GGATTTCCCT 
ACCTTATTAA CTCCTGGGAT GATGGAAAAT CTCTTCCATG AAATGGGGCA TGCCATGCAC 
TCAATGCTCG GACGAACTCG GTACCAGCAT GTCACTGGGA CCAGGTGCCC TACTGACTTT 
GCTGAAGTTC CGTCTATTCT GATGGAGTAT TTTTCAAATG ATTACCGAGT AGTCAGCCAG 
TTTGCCAAGC ATTATCAGAC TGGTCAGCCA CTGCCGAAAG CTATGGTGTC TCGTCTCTGT 
GAATCTAAGA AGGTCTGTGC CGCCGCAGAA ATGCAGCTGC AGGTCTTTTA TGCCGCCCTG 
GATCAAATCT ACCATGGCCA GCATCCCCTG AAGAAGTCAA CCACAGACAT CCTCATGGAA 
ACACAAGAGC AGTTTTATGG CTTACCCTAT GTTCCTGACA CTGCCTGGCA GCTGAGGTTC 
AGCCACCTGG TGGGATATGG CGCCAAGTAT TACTCATACC TCATGTCCAG GGCTGTGGCA 
TCCATGGTCT GGAAGGAGTG CTTCCTACAG GATCCCTTTA ACAGGGCCGC CGGGGAGCGC 
TACCGCAGAG AGATGTTAGC CCACGGTGGG GGCAAGGAAC CTATGCTCAT GATTCAAGGC 
ATGCTGCAGA AATGTCCTTC CATCGATGAT TTTGTGGATG CCCTTGTGTC TGACTTGAAT 
CTGGACTTTG AAACTTTCTT CATGGATTCT AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mipep ( XM_008770779.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769001.2 Rattus norvegicus mitochondrial intermediate peptidase (Mipep), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58653
Clone ID Related Accession (Same CDS sequence)	XM_008770779.2
Accession Version	XM_008770779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial intermediate peptidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGG CAGCCGGGAC GCGGTATGCC TACCGGCTGT GCGGCCGGCG AGCCGCGGCG 
GCTCTGCAGG GACACGCAGG CCGGAGCTGC GCCCGGAGCG TTAGCACTAG CTGGTCCCCG 
GTGGGCGCTG CCTTTAACGT CAAGCCCCAG GGCCACCTGT GGGACCTGTT GGGAGAGCGA 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAGGAG 
GAAGCCTTGA GGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC TCCAGGGCCA 
CAGACAGTCC TCATTTTCGA TGAGCTCTCG GATTGCCTCT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGAGCAG GCGTTCAGAG AGGCTGCGCA GGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACGAATG TGGAATTGTA TCAGAGTCTG 
CAGAAATTAC TAGATGACAA GAAGCTTATG GATTCCCTTG ATGCAGAAAC CAGGCGAGTA 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCCG TGGACCTCAA TGTTAAGATC TTGGACTTGA GTAGTGCTTT CCTCATGGGA 
ACCAACTTTC CCATCAAGAT CCAGAAGCAT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCTCGTGATG GAAGGCATTT AGTCATCGAT GGTCTACATG CGGAAGCCTC CGATGACCTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CTGATCAGTT GAAGTGTTTG 
GAGGAGTTGC TAAGCAGCCG AGATCTTCTG GCAAACCTAG TAGGCTATTC TACCTTTTCC 
CACAGGGCCC TCCAGGGAAC CATAGCCCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTG CGAAAGAACT AGAAAAGATT TTGAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTGAA CTAATGCCTT GGGACCCTCC CTACTACAGT 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATATTGTCC ATTCTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC CTATTTAACA GACTGTTGGG CGTCACCTTA 
TATGCAGAAC AGCCCTTCAA AGGAGAGGTG TGGTGTATTG ATGTCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGTT GCTGGGCTAC ATTTACTGTG ATTTTTTCCA GCGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGGGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTCC CAGTTGTGGT TCTCATGCTG AATCTCCCTC ATGCTTCCAG GGATTTCCCT 
ACCTTATTAA CTCCTGGGAT GATGGAAAAT CTCTTCCATG AAATGGGGCA TGCCATGCAC 
TCAATGCTCG GACGAACTCG GTACCAGCAT GTCACTGGGA CCAGGTGCCC TACTGACTTT 
GCTGAAGTTC CGTCTATTCT GATGGAGTAT TTTTCAAATG ATTACCGAGT AGTCAGCCAG 
TTTGCCAAGC ATTATCAGAC TGGTCAGGTC TTTTATGCCG CCCTGGATCA AATCTACCAT 
GGCCAGCATC CCCTGAAGAA GTCAACCACA GACATCCTCA TGGAAACACA AGAGCAGTTT 
TATGGCTTAC CCTATGTTCC TGACACTGCC TGGCAGCTGA GGTTCAGCCA CCTGGTGGGA 
TATGGCGCCA AGTATTACTC ATACCTCATG TCCAGGGCTG TGGCATCCAT GGTCTGGAAG 
GAGTGCTTCC TACAGGATCC CTTTAACAGG GCCGCCGGGG AGCGCTACCG CAGAGAGATG 
TTAGCCCACG GTGGGGGCAA GGAACCTATG CTCATGATTC AAGGCATGCT GCAGAAATGT 
CCTTCCATCG ATGATTTTGT GGATGCCCTT GTGTCTGACT TGAATCTGGA CTTTGAAACT 
TTCTTCATGG ATTCTAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769001.2
CDS	122..2182
Translation	MLLAAGTRYAYRLCGRRAAAALQGHAGRSCARSVSTSWSPVGAAFNVKPQGHLWDLLGERRGLFGVPELSTPEGFQVAQEEALRKTEWLVERACSTPPGPQTVLIFDELSDCLCRVADLADFVKIGHPEQAFREAAQEACRSIGTMVEKLNTNVELYQSLQKLLDDKKLMDSLDAETRRVAELFMFDFEISGIHLDEEKRRRAVDLNVKILDLSSAFLMGTNFPIKIQKHLLPEHIQHHFARDGRHLVIDGLHAEASDDLVREAAYKIFLYPNADQLKCLEELLSSRDLLANLVGYSTFSHRALQGTIAQTPETVMQFLEKLSEKLCERTRKDFEMMQGMKTKLNPQNSELMPWDPPYYSGVIRAERYNIEPSLYCPFLSLGACMEGLNVLFNRLLGVTLYAEQPFKGEVWCIDVRKLAVVHESEGLLGYIYCDFFQRANKPQQDCHFTIRGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTPGMMENLFHEMGHAMHSMLGRTRYQHVTGTRCPTDFAEVPSILMEYFSNDYRVVSQFAKHYQTGQVFYAALDQIYHGQHPLKKSTTDILMETQEQFYGLPYVPDTAWQLRFSHLVGYGAKYYSYLMSRAVASMVWKECFLQDPFNRAAGERYRREMLAHGGGKEPMLMIQGMLQKCPSIDDFVDALVSDLNLDFETFFMDSK

Target ORF information:

RefSeq Version	XM_008770779.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitochondrial intermediate peptidase (Mipep), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770779.2

ORF Insert Sequence:

ATGCTGCTGG CAGCCGGGAC GCGGTATGCC TACCGGCTGT GCGGCCGGCG AGCCGCGGCG 
GCTCTGCAGG GACACGCAGG CCGGAGCTGC GCCCGGAGCG TTAGCACTAG CTGGTCCCCG 
GTGGGCGCTG CCTTTAACGT CAAGCCCCAG GGCCACCTGT GGGACCTGTT GGGAGAGCGA 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAGGAG 
GAAGCCTTGA GGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC TCCAGGGCCA 
CAGACAGTCC TCATTTTCGA TGAGCTCTCG GATTGCCTCT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGAGCAG GCGTTCAGAG AGGCTGCGCA GGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACGAATG TGGAATTGTA TCAGAGTCTG 
CAGAAATTAC TAGATGACAA GAAGCTTATG GATTCCCTTG ATGCAGAAAC CAGGCGAGTA 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCCG TGGACCTCAA TGTTAAGATC TTGGACTTGA GTAGTGCTTT CCTCATGGGA 
ACCAACTTTC CCATCAAGAT CCAGAAGCAT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCTCGTGATG GAAGGCATTT AGTCATCGAT GGTCTACATG CGGAAGCCTC CGATGACCTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CTGATCAGTT GAAGTGTTTG 
GAGGAGTTGC TAAGCAGCCG AGATCTTCTG GCAAACCTAG TAGGCTATTC TACCTTTTCC 
CACAGGGCCC TCCAGGGAAC CATAGCCCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTG CGAAAGAACT AGAAAAGATT TTGAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTGAA CTAATGCCTT GGGACCCTCC CTACTACAGT 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATATTGTCC ATTCTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC CTATTTAACA GACTGTTGGG CGTCACCTTA 
TATGCAGAAC AGCCCTTCAA AGGAGAGGTG TGGTGTATTG ATGTCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGTT GCTGGGCTAC ATTTACTGTG ATTTTTTCCA GCGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGGGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTCC CAGTTGTGGT TCTCATGCTG AATCTCCCTC ATGCTTCCAG GGATTTCCCT 
ACCTTATTAA CTCCTGGGAT GATGGAAAAT CTCTTCCATG AAATGGGGCA TGCCATGCAC 
TCAATGCTCG GACGAACTCG GTACCAGCAT GTCACTGGGA CCAGGTGCCC TACTGACTTT 
GCTGAAGTTC CGTCTATTCT GATGGAGTAT TTTTCAAATG ATTACCGAGT AGTCAGCCAG 
TTTGCCAAGC ATTATCAGAC TGGTCAGGTC TTTTATGCCG CCCTGGATCA AATCTACCAT 
GGCCAGCATC CCCTGAAGAA GTCAACCACA GACATCCTCA TGGAAACACA AGAGCAGTTT 
TATGGCTTAC CCTATGTTCC TGACACTGCC TGGCAGCTGA GGTTCAGCCA CCTGGTGGGA 
TATGGCGCCA AGTATTACTC ATACCTCATG TCCAGGGCTG TGGCATCCAT GGTCTGGAAG 
GAGTGCTTCC TACAGGATCC CTTTAACAGG GCCGCCGGGG AGCGCTACCG CAGAGAGATG 
TTAGCCCACG GTGGGGGCAA GGAACCTATG CTCATGATTC AAGGCATGCT GCAGAAATGT 
CCTTCCATCG ATGATTTTGT GGATGCCCTT GTGTCTGACT TGAATCTGGA CTTTGAAACT 
TTCTTCATGG ATTCTAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mipep ( XM_008770780.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769002.2 Rattus norvegicus mitochondrial intermediate peptidase (Mipep), transcript variant X2, mRNA.

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CloneID	ORa58654
Clone ID Related Accession (Same CDS sequence)	XM_008770780.2
Accession Version	XM_008770780.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial intermediate peptidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGG CAGCCGGGAC GCGGTATGCC TACCGGCTGT GCGGCCGGCG AGCCGCGGCG 
GCTCTGCAGG GACACGCAGG CCGGAGCTGC GCCCGGAGCG TTAGCACTAG CTGGTCCCCG 
GTGGGCGCTG CCTTTAACGT CAAGCCCCAG GGCCACCTGT GGGACCTGTT GGGAGAGCGA 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAGGAG 
GAAGCCTTGA GGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC TCCAGGGCCA 
CAGACAGTCC TCATTTTCGA TGAGCTCTCG GATTGCCTCT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGAGCAG GCGTTCAGAG AGGCTGCGCA GGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACGAATG TGGAATTGTA TCAGAGTCTG 
CAGAAATTAC TAGATGACAA GAAGCTTATG GATTCCCTTG ATGCAGAAAC CAGGCGAGTA 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCCG TGGACCTCAA TGTTAAGATC TTGGACTTGA GTAGTGCTTT CCTCATGGGA 
ACCAACTTTC CCATCAAGAT CCAGAAGCAT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCTCGTGATG GAAGGCATTT AGTCATCGAT GGTCTACATG CGGAAGCCTC CGATGACCTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CTGATCAGTT GAAGTGTTTG 
GAGGAGTTGC TAAGCAGCCG AGATCTTCTG GCAAACCTAG TAGGCTATTC TACCTTTTCC 
CACAGGGCCC TCCAGGGAAC CATAGCCCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTG CGAAAGAACT AGAAAAGATT TTGAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTGAA CTAATGCCTT GGGACCCTCC CTACTACAGT 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATATTGTCC ATTCTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC CTATTTAACA GACTGTTGGG CGTCACCTTA 
TATGCAGAAC AGCCCTTCAA AGGAGAGGTG TGGTGTATTG ATGTCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGTT GCTGGGCTAC ATTTACTGTG ATTTTTTCCA GCGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGGGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTCC CAGTTGTGGT TCTCATGCTG AATCTCCCTC ATGCTTCCAG GGATTTCCCT 
ACCTTATTAA CTCCTGGGAT GATGGAAAAT CTCTTCCATG AAATGGGGCA TGCCATGCAC 
TCAATGCTCG GACGAACTCG GTACCAGCAT GTCACTGGGA CCAGGTGCCC TACTGACTTT 
GCTGAAGTTC CGTCTATTCT GATGGAGTAT TTTTCAAATG ATTACCGAGT AGTCAGCCAG 
TTTGCCAAGC ATTATCAGAC TGGTCAGCCA CTGCCGAAAG CTATGGTGTC TCGTCTCTGT 
GAATCTAAGA AGGTCTGTGC CGCCGCAGAA ATGCAGCTGC AGGTCTTTTA TGCCGCCCTG 
GATCAAATCT ACCATGGCCA GCATCCCCTG AAGAAGTCAA CCACAGACAT CCTCATGGAA 
ACACAAGAGC AGTTTTATGG CTTACCCTAT GTTCCTGACA CTCAAAACCG ATGGTGGCCC 
GAACCAGGAC AGTGGTTGCA TGTAAAGGAT GGTGCAGAAG CAGAGGTAAG CTCTAGGACT 
GGGCCAGATG TGCAGGTTGG GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769002.2
CDS	122..2068
Translation	MLLAAGTRYAYRLCGRRAAAALQGHAGRSCARSVSTSWSPVGAAFNVKPQGHLWDLLGERRGLFGVPELSTPEGFQVAQEEALRKTEWLVERACSTPPGPQTVLIFDELSDCLCRVADLADFVKIGHPEQAFREAAQEACRSIGTMVEKLNTNVELYQSLQKLLDDKKLMDSLDAETRRVAELFMFDFEISGIHLDEEKRRRAVDLNVKILDLSSAFLMGTNFPIKIQKHLLPEHIQHHFARDGRHLVIDGLHAEASDDLVREAAYKIFLYPNADQLKCLEELLSSRDLLANLVGYSTFSHRALQGTIAQTPETVMQFLEKLSEKLCERTRKDFEMMQGMKTKLNPQNSELMPWDPPYYSGVIRAERYNIEPSLYCPFLSLGACMEGLNVLFNRLLGVTLYAEQPFKGEVWCIDVRKLAVVHESEGLLGYIYCDFFQRANKPQQDCHFTIRGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTPGMMENLFHEMGHAMHSMLGRTRYQHVTGTRCPTDFAEVPSILMEYFSNDYRVVSQFAKHYQTGQPLPKAMVSRLCESKKVCAAAEMQLQVFYAALDQIYHGQHPLKKSTTDILMETQEQFYGLPYVPDTQNRWWPEPGQWLHVKDGAEAEVSSRTGPDVQVGC

Target ORF information:

RefSeq Version	XM_008770780.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitochondrial intermediate peptidase (Mipep), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770780.2

ORF Insert Sequence:

ATGCTGCTGG CAGCCGGGAC GCGGTATGCC TACCGGCTGT GCGGCCGGCG AGCCGCGGCG 
GCTCTGCAGG GACACGCAGG CCGGAGCTGC GCCCGGAGCG TTAGCACTAG CTGGTCCCCG 
GTGGGCGCTG CCTTTAACGT CAAGCCCCAG GGCCACCTGT GGGACCTGTT GGGAGAGCGA 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAGGAG 
GAAGCCTTGA GGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC TCCAGGGCCA 
CAGACAGTCC TCATTTTCGA TGAGCTCTCG GATTGCCTCT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGAGCAG GCGTTCAGAG AGGCTGCGCA GGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACGAATG TGGAATTGTA TCAGAGTCTG 
CAGAAATTAC TAGATGACAA GAAGCTTATG GATTCCCTTG ATGCAGAAAC CAGGCGAGTA 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCCG TGGACCTCAA TGTTAAGATC TTGGACTTGA GTAGTGCTTT CCTCATGGGA 
ACCAACTTTC CCATCAAGAT CCAGAAGCAT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCTCGTGATG GAAGGCATTT AGTCATCGAT GGTCTACATG CGGAAGCCTC CGATGACCTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CTGATCAGTT GAAGTGTTTG 
GAGGAGTTGC TAAGCAGCCG AGATCTTCTG GCAAACCTAG TAGGCTATTC TACCTTTTCC 
CACAGGGCCC TCCAGGGAAC CATAGCCCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTG CGAAAGAACT AGAAAAGATT TTGAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTGAA CTAATGCCTT GGGACCCTCC CTACTACAGT 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATATTGTCC ATTCTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC CTATTTAACA GACTGTTGGG CGTCACCTTA 
TATGCAGAAC AGCCCTTCAA AGGAGAGGTG TGGTGTATTG ATGTCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGTT GCTGGGCTAC ATTTACTGTG ATTTTTTCCA GCGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGGGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTCC CAGTTGTGGT TCTCATGCTG AATCTCCCTC ATGCTTCCAG GGATTTCCCT 
ACCTTATTAA CTCCTGGGAT GATGGAAAAT CTCTTCCATG AAATGGGGCA TGCCATGCAC 
TCAATGCTCG GACGAACTCG GTACCAGCAT GTCACTGGGA CCAGGTGCCC TACTGACTTT 
GCTGAAGTTC CGTCTATTCT GATGGAGTAT TTTTCAAATG ATTACCGAGT AGTCAGCCAG 
TTTGCCAAGC ATTATCAGAC TGGTCAGCCA CTGCCGAAAG CTATGGTGTC TCGTCTCTGT 
GAATCTAAGA AGGTCTGTGC CGCCGCAGAA ATGCAGCTGC AGGTCTTTTA TGCCGCCCTG 
GATCAAATCT ACCATGGCCA GCATCCCCTG AAGAAGTCAA CCACAGACAT CCTCATGGAA 
ACACAAGAGC AGTTTTATGG CTTACCCTAT GTTCCTGACA CTCAAAACCG ATGGTGGCCC 
GAACCAGGAC AGTGGTTGCA TGTAAAGGAT GGTGCAGAAG CAGAGGTAAG CTCTAGGACT 
GGGCCAGATG TGCAGGTTGG GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Processing of mitochondrial presequences.
Biochimica et biophysica acta1819(9-10)1098-106(2012 Sep-Oct)
Mossmann D,Meisinger C,V?gtle FN

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

Sequence analysis of rat mitochondrial intermediate peptidase: similarity to zinc metallopeptidases and to a putative yeast homologue.
Proceedings of the National Academy of Sciences of the United States of America89(17)8317-21(1992 Sep)
Isaya G,Kalousek F,Rosenberg LE

Rat liver mitochondrial intermediate peptidase (MIP): purification and initial characterization.
The EMBO journal11(8)2803-9(1992 Aug)
Kalousek F,Isaya G,Rosenberg LE

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Mipep cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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