Itpka cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itpka
Entrez Gene ID	81677
Full Name	inositol-trisphosphate 3-kinase A
General protein information	Preferred Names inositol-trisphosphate 3-kinase A Names inositol-trisphosphate 3-kinase A IP3 3-kinase A IP3K A inositol 1,4,5-triphosphate 3-kinase inositol 1,4,5-trisphosphate 3-kinase A inositol 145-triphosphate 3-kinase insP 3-kinase A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35
Summary	an enzyme that catalyses the phosphorylation of InsP3 to inositol 1,3,4,5-tetrakisphosphate (InsP4) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031045.2	NP_112307.2	inositol-trisphosphate 3-kinase A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ITPKA	NP_001076988.1
Xenopus tropicalis (tropical clawed frog)	LOC100486229	XP_002935396.2
	ITPKA	XP_001099176.2
Rattus norvegicus (Norway rat)	Itpka	NP_112307.2
Danio rerio (zebrafish)	LOC564528	XP_692949.1
Homo sapiens (human)	ITPKA	NP_002211.1
Pan troglodytes (chimpanzee)	ITPKA	XP_001149577.1
Canis lupus familiaris (dog)	ITPKA	XP_544631.3
Mus musculus (house mouse)	Itpka	NP_666237.1
Gallus gallus (chicken)	ITPKA	NP_990212.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002194	XP_565625.4

Gene Ontology
Bibliography

Related articles in PubMed

Inositol 1,4,5-trisphosphate 3-kinase A is a novel microtubule-associated protein: PKA-dependent phosphoregulation of microtubule binding affinity.
Lee D, Lee HW, Hong S, Choi BI, Kim HW, Han SB, Kim IH, Bae JY, Bae YC, Rhyu IJ, Sun W, Kim H
The Journal of biological chemistry287(19)15981-95(2012 May)

IP3 3-kinase opposes NGF driven neurite outgrowth.
Eva R, Bouyoucef-Cherchalli D, Patel K, Cullen PJ, Banting G
PloS one7(2)e32386(2012)

Neuronal IP3 3-kinase is an F-actin-bundling protein: role in dendritic targeting and regulation of spine morphology.
Johnson HW, Schell MJ
Molecular biology of the cell20(24)5166-80(2009 Dec)

Inositol 1,4,5-trisphosphate 3-kinase a functions as a scaffold for synaptic Rac signaling.
Kim IH, Park SK, Hong ST, Jo YS, Kim EJ, Park EH, Han SB, Shin HS, Sun W, Kim HT, Soderling SH, Kim H
The Journal of neuroscience : the official journal of the Society for Neuroscience29(44)14039-49(2009 Nov)

Translational mobility of the type 3 inositol 1,4,5-trisphosphate receptor Ca2+ release channel in endoplasmic reticulum membrane.
Ferreri-Jacobia M, Mak DO, Foskett JK
The Journal of biological chemistry280(5)3824-31(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that protein kinse A (PKA) phosphorylated Ser-119 within inositol 1,4,5-trisphosphate 3-kinase A (IP(3)K-A), leading to a significant reduction of microtubule binding affinity.
Title: Inositol 1,4,5-trisphosphate 3-kinase A is a novel microtubule-associated protein: PKA-dependent phosphoregulation of microtubule binding affinity.

IP3 3-kinase opposes NGF driven neurite outgrowth
Title: IP3 3-kinase opposes NGF driven neurite outgrowth.

These results suggest that, in addition to its ability to regulate intracellular Ca(2+) via Ins(1,4,5)P(3) metabolism, ITPKA regulates structural plasticity.
Title: Neuronal IP3 3-kinase is an F-actin-bundling protein: role in dendritic targeting and regulation of spine morphology.

the large majority of InsP3R3 channels free to diffuse throughout the endoplasmic reticulum, at rates that are comparable with those measured for other polytopic ER integral membrane proteins
Title: Translational mobility of the type 3 inositol 1,4,5-trisphosphate receptor Ca2+ release channel in endoplasmic reticulum membrane.

plays a role in the processing of spatial memory, most likely by regulating the calcium signaling in the dendritic spines of hippocampal formation
Title: Spatial learning enhances the expression of inositol 1,4,5-trisphosphate 3-kinase A in the hippocampal formation of rat.

The following Itpka gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itpka cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07453	NM_031045.2 Latest version!	Rattus norvegicus inositol-trisphosphate 3-kinase A (Itpka), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itpka ( NM_031045.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112307.2 Rattus norvegicus inositol-trisphosphate 3-kinase A (Itpka), mRNA.

Price & Availability↑

CloneID	ORa07453
Clone ID Related Accession (Same CDS sequence)	NM_031045.2
Accession Version	NM_031045.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	inositol-trisphosphate 3-kinase A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M29787.1, AC107565.6 and BQ204027.1. On Aug 4, 2012 this sequence version replaced NM_031045.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M29787.1, X56917.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACCCTGC CCGGACACCC GACGGGCATG GCGCGACCAC GGGGCGCGGG GCCCTGCAGC 
CCCGGGTTGG AGCGGGCTCC GCGCCGGAGC GTCGGGGAGC TGCGCCTGCT CTTCGAAGCG 
CGCTGCGCCG CAGTCGCCGC CGCAGCGGCC GCAGGGGAGC CCCGGGCCCG CGGGGCCAAG 
CGGCGTGGGG GACAAGTGCC TAACGGGCTC CCGCGTGCTG CCCCTGCTCC GGTGATCCCG 
CAGCTCACTG TGACAAGCGA GGAGGATGTG GCCCCGGCCA GCCCCGGGCC GCCGGACCGG 
GAGGGGAACT GGCTCCCAGC TGCGGGGTCG CACCTGCAGC AGCCACGCCG CCTCTCCACC 
TCGTCCCTCT CCTCCACCGG CTCCTCGTCG CTGCTCGAGG ACTCGGAGGA CGACCTGCTG 
AGCGACAGCG AGAGCCGGAG CCGCGGCAAC GTGCAGCTGG AAACCAGCGA AGACGTGGGG 
CAGAAAAGCC ATTGGCAGAA GATCCGTACC ATGGTCAACC TGCCTGTCAT GAGTCCTTTC 
AAAAAGCGCT ACTCCTGGGT GCAGTTAGCA GGGCACACAG GGAGTTTCAA AGCTGCGGGC 
ACCAGCGGCC TGATCCTGAA GCGCAGCTCG GAGCCTGAAC ACTACTGCCT GGTGCGGTTG 
ATGGCTGATG TGCTGCGTGG GTGTGTGCCG GCCTTCCACG GTGTGGTCGA GCGGGATGGT 
GAAAGCTACT TACAGTTACA GGACCTGCTT GATGGCTTCG ATGGACCTTG CGTGCTTGAC 
TGCAAGATGG GTGTCAGAAC TTACCTGGAA GAAGAGCTGA CCAAAGCCCG AGAACGGCCC 
AAGCTGCGAA AGGACATGTA TAAGAAAATG CTGGCCGTGG ACCCCGAGGC ACCTACTGAG 
GAGGAGCATG CGCAGCGTGC CGTCACAAAA CCTCGATACA TGCAATGGCG CGAAGGCATC 
AGCTCCAGCA CTACACTCGG CTTCCGCATC GAGGGCATCA AGAAAGCCGA TGGATCTTGC 
AGTACTGATT TCAAAACTAC ACGAAGCAGA GAGCAAGTGA CTCGAGTCTT TGAGGAGTTC 
ATGCAAGGAG ATGCTGAAGT GCTGAAGAGG TATCTGAACC GCCTACAGCA GATCCGGGAT 
ACCCTGGAGA TCTCTGATTT CTTTAGACGG CACGAGGTGA TTGGCAGCTC ACTCCTCTTC 
GTGCATGACC ATTGCCATCG CGCTGGTGTG TGGCTCATCG ATTTTGGCAA GACCACGCCT 
CTCCCCGATG GCCAGATCCT GGATCATCGG AGGCCCTGGG AGGAGGGCAA TCGTGAGGAC 
GGCTATTTGC TGGGGCTGGA CAATCTCATT GGCATCTTGG CCAACCTGGC TGAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112307.2
CDS	46..1425
Translation	MTLPGHPTGMARPRGAGPCSPGLERAPRRSVGELRLLFEARCAAVAAAAAAGEPRARGAKRRGGQVPNGLPRAAPAPVIPQLTVTSEEDVAPASPGPPDREGNWLPAAGSHLQQPRRLSTSSLSSTGSSSLLEDSEDDLLSDSESRSRGNVQLETSEDVGQKSHWQKIRTMVNLPVMSPFKKRYSWVQLAGHTGSFKAAGTSGLILKRSSEPEHYCLVRLMADVLRGCVPAFHGVVERDGESYLQLQDLLDGFDGPCVLDCKMGVRTYLEEELTKARERPKLRKDMYKKMLAVDPEAPTEEEHAQRAVTKPRYMQWREGISSSTTLGFRIEGIKKADGSCSTDFKTTRSREQVTRVFEEFMQGDAEVLKRYLNRLQQIRDTLEISDFFRRHEVIGSSLLFVHDHCHRAGVWLIDFGKTTPLPDGQILDHRRPWEEGNREDGYLLGLDNLIGILANLAER

Target ORF information:

RefSeq Version	NM_031045.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus inositol-trisphosphate 3-kinase A (Itpka), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031045.2

ORF Insert Sequence:

ATGACCCTGC CCGGACACCC GACGGGCATG GCGCGACCAC GGGGCGCGGG GCCCTGCAGC 
CCCGGGTTGG AGCGGGCTCC GCGCCGGAGC GTCGGGGAGC TGCGCCTGCT CTTCGAAGCG 
CGCTGCGCCG CAGTCGCCGC CGCAGCGGCC GCAGGGGAGC CCCGGGCCCG CGGGGCCAAG 
CGGCGTGGGG GACAAGTGCC TAACGGGCTC CCGCGTGCTG CCCCTGCTCC GGTGATCCCG 
CAGCTCACTG TGACAAGCGA GGAGGATGTG GCCCCGGCCA GCCCCGGGCC GCCGGACCGG 
GAGGGGAACT GGCTCCCAGC TGCGGGGTCG CACCTGCAGC AGCCACGCCG CCTCTCCACC 
TCGTCCCTCT CCTCCACCGG CTCCTCGTCG CTGCTCGAGG ACTCGGAGGA CGACCTGCTG 
AGCGACAGCG AGAGCCGGAG CCGCGGCAAC GTGCAGCTGG AAACCAGCGA AGACGTGGGG 
CAGAAAAGCC ATTGGCAGAA GATCCGTACC ATGGTCAACC TGCCTGTCAT GAGTCCTTTC 
AAAAAGCGCT ACTCCTGGGT GCAGTTAGCA GGGCACACAG GGAGTTTCAA AGCTGCGGGC 
ACCAGCGGCC TGATCCTGAA GCGCAGCTCG GAGCCTGAAC ACTACTGCCT GGTGCGGTTG 
ATGGCTGATG TGCTGCGTGG GTGTGTGCCG GCCTTCCACG GTGTGGTCGA GCGGGATGGT 
GAAAGCTACT TACAGTTACA GGACCTGCTT GATGGCTTCG ATGGACCTTG CGTGCTTGAC 
TGCAAGATGG GTGTCAGAAC TTACCTGGAA GAAGAGCTGA CCAAAGCCCG AGAACGGCCC 
AAGCTGCGAA AGGACATGTA TAAGAAAATG CTGGCCGTGG ACCCCGAGGC ACCTACTGAG 
GAGGAGCATG CGCAGCGTGC CGTCACAAAA CCTCGATACA TGCAATGGCG CGAAGGCATC 
AGCTCCAGCA CTACACTCGG CTTCCGCATC GAGGGCATCA AGAAAGCCGA TGGATCTTGC 
AGTACTGATT TCAAAACTAC ACGAAGCAGA GAGCAAGTGA CTCGAGTCTT TGAGGAGTTC 
ATGCAAGGAG ATGCTGAAGT GCTGAAGAGG TATCTGAACC GCCTACAGCA GATCCGGGAT 
ACCCTGGAGA TCTCTGATTT CTTTAGACGG CACGAGGTGA TTGGCAGCTC ACTCCTCTTC 
GTGCATGACC ATTGCCATCG CGCTGGTGTG TGGCTCATCG ATTTTGGCAA GACCACGCCT 
CTCCCCGATG GCCAGATCCT GGATCATCGG AGGCCCTGGG AGGAGGGCAA TCGTGAGGAC 
GGCTATTTGC TGGGGCTGGA CAATCTCATT GGCATCTTGG CCAACCTGGC TGAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inositol 1,4,5-trisphosphate 3-kinase A is a novel microtubule-associated protein: PKA-dependent phosphoregulation of microtubule binding affinity.
The Journal of biological chemistry287(19)15981-95(2012 May)
Lee D,Lee HW,Hong S,Choi BI,Kim HW,Han SB,Kim IH,Bae JY,Bae YC,Rhyu IJ,Sun W,Kim H

IP3 3-kinase opposes NGF driven neurite outgrowth.
PloS one7(2)e32386(2012)
Eva R,Bouyoucef-Cherchalli D,Patel K,Cullen PJ,Banting G

Neuronal IP3 3-kinase is an F-actin-bundling protein: role in dendritic targeting and regulation of spine morphology.
Molecular biology of the cell20(24)5166-80(2009 Dec)
Johnson HW,Schell MJ

Inositol 1,4,5-trisphosphate 3-kinase a functions as a scaffold for synaptic Rac signaling.
The Journal of neuroscience : the official journal of the Society for Neuroscience29(44)14039-49(2009 Nov)
Kim IH,Park SK,Hong ST,Jo YS,Kim EJ,Park EH,Han SB,Shin HS,Sun W,Kim HT,Soderling SH,Kim H

Translational mobility of the type 3 inositol 1,4,5-trisphosphate receptor Ca2+ release channel in endoplasmic reticulum membrane.
The Journal of biological chemistry280(5)3824-31(2005 Feb)
Ferreri-Jacobia M,Mak DO,Foskett JK