Gpt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpt
Entrez Gene ID	81670
Full Name	glutamic--pyruvic transaminase
Synonyms	ALAT,Gpt1
General protein information	Preferred Names glutamic--pyruvic transaminase Names alanine aminotransferase 1 ALT1 GPT 1 glutamate pyruvate transaminase 1 glutamic pyruvate transaminase glutamic pyruvic transaminase (alanine aminotrasferase) glutamic pyruvic transaminase 1, soluble glutamic--alanine transaminase 1 glutamic--pyruvic transaminase 1 glutamic-pyruvate transaminase (alanine aminotransferase)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	human homolog is a cytosolic alanine aminotransferase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031039.1	NP_112301.1	alanine aminotransferase 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:ch211-217a12.1	XP_001919896.1
Saccharomyces cerevisiae (baker's yeast)	ALT2	NP_010396.1
Arabidopsis thaliana (thale cress)	AOAT2	NP_974122.1
Homo sapiens (human)	GPT	NP_005300.1
	GPT	XP_001093616.1
Bos taurus (cattle)	GPT	NP_001077209.1
Rattus norvegicus (Norway rat)	Gpt	NP_112301.1
Danio rerio (zebrafish)	gpt2l	NP_001136246.1
Arabidopsis thaliana (thale cress)	GGT1	NP_564192.2
Canis lupus familiaris (dog)	GPT	XP_005628374.1
Mus musculus (house mouse)	Gpt	NP_877957.1
Pan troglodytes (chimpanzee)	GPT	XP_003951417.1
Eremothecium gossypii	AGOS_AGR085W	NP_986750.1
Oryza sativa (rice)	Os07g0108300	NP_001058716.1
Xenopus tropicalis (tropical clawed frog)	gpt2	NP_001016805.1

Gene Ontology
Bibliography

Related articles in PubMed

Hepatitis B virus e antigen-negative chronic infection. Treatment based on glutamic pyruvic transaminase and hepatitis B virus deoxyribonucleic acid cut-off values.
Guardiola-Ar?valo A, G?mez Rodr?guez R, Romero Guti?rrez M, G?mez Moreno AZ, Garc?a Vela A, S?nchez Sim?n R, G?mez Hernando C, Andr?s Esteban EM
Gastroenterologia y hepatologia(2017 Dec)

Association between MFN2 gene polymorphisms and the risk and prognosis of acute liver failure: a case-control study in a Chinese population.
Wei YL, Tian Q, Zhao XX, Qiu GZ, Xu Y
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas50(6)e5758(2017 May)

Effects of saikosaponins on hepatic damage induced by halothane and hypoxia in phenobarbital-pretreated rats.
Nishiura T, Marukawa S, Ishida H, Orita M, Abe H
Journal of anesthesia8(1)87-92(1994 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that AZD4619 increased alanine aminotransferase (ALT1) protein expression in a dose-dependent manner in human, but not in rat hepatocytes.
Title: The peroxisome proliferator-activated receptor?? agonist, AZD4619, induces alanine aminotransferase-1 gene and protein expression in human, but not in rat hepatocytes: Correlation with serum ALT levels.

Data suggest that both Alt1 and Alt2 are induced in liver during gluconeogenesis and in diabetes (Goto-Kakizaki rats); in hepatocytes, both Alt1 and Alt2 are induced by glucocorticoid dexamethasone and are suppressed by insulin.
Title: Hepatic ALT isoenzymes are elevated in gluconeogenic conditions including diabetes and suppressed by insulin at the protein level.

Protein levels are significantly elevated and correlate with activity, providing the molecular basis for the elevated total serum enzyme activity in liver toxicity.
Title: Alanine aminotransferase isoenzymes: molecular cloning and quantitative analysis of tissue expression in rats and serum elevation in liver toxicity.

Report the early effects of short-term dexamethasone administration on hepatic and serum alanine aminotransferase in the rat.
Title: The early effects of short-term dexamethasone administration on hepatic and serum alanine aminotransferase in the rat.

Although ALT and GMT activities and TNF-alpha and IL-6 concentrations were higher in male rats histopathological findings have shown slightly more advanced fibrotic and inflammatory changes in female rats.
Title: The differences in selected biochemical markers and histological findings after bile duct ligation in male and female rats.

The following Gpt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07437	NM_031039.1 Latest version!	Rattus norvegicus glutamic--pyruvic transaminase (Gpt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$293.30 $419.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpt ( NM_031039.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112301.1 Rattus norvegicus glutamic--pyruvic transaminase (Gpt), mRNA.

Price & Availability↑

CloneID	ORa07437
Clone ID Related Accession (Same CDS sequence)	NM_031039.1
Accession Version	NM_031039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Rattus norvegicus(Norway rat)
Product	alanine aminotransferase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D10354.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D10354.1, BC097937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCAC GGGTGAATGA TCAAAGCCAG GCTTCAAGGA ATGGGCTGAA GGGAAAGGTG 
CTAACTCTGG ACACTATGAA CCCATGTGTG CGGAGGGTGG AGTATGCAGT TCGAGGACCC 
ATTGTGCAGC GTGCCTTGGA GCTGGAGCAG GAGCTGCGTC AGGGTGTGAA GAAGCCGTTT 
ACTGAGGTCA TCCGTGCCAA CATTGGGGAT GCACAAGCCA TGGGGCAGAG ACCCATCACC 
TTCTTCCGCC AGGTCCTGGC CCTCTGTGTC TACCCCAATC TTCTGAGCAG TCCTGACTTC 
CCAGAGGATG CCAAGAGAAG GGCAGAACGC ATCTTGCAGG CCTGCGGGGG CCACAGCCTG 
GGTGCCTATA GCATTAGCTC TGGAATCCAG CCGATCCGGG AGGATGTGGC GCAATACATT 
GAGAGAAGAG ACGGAGGCAT CCCCGCAGAC CCGAACAACA TATTTCTATC CACAGGGGCC 
AGCGATGCCA TCGTGACAAT GCTCAAGCTG CTGGTATCTG GCGAGGGCCG TGCACGAACA 
GGTGTACTCA TTCCCATTCC TCAGTACCCA CTGTACTCAG CCGCGCTGGC TGAACTGGAC 
GCCGTGCAAG TGGACTACTA CCTGGACGAA GAGCGCGCCT GGGCTCTGGA CATCGCAGAG 
CTGCGGCGCG CTCTGTGCCA GGCACGTGAC CGTTGCTGCC CTCGAGTACT GTGCGTCATC 
AACCCCGGCA ACCCCACTGG GCAGGTGCAG ACCCGTGAGT GCATCGAGGC CGTAATCCGC 
TTTGCTTTCA AAGAAGGACT CTTCTTGATG GCTGATGAGG TATACCAGGA CAACGTGTAT 
GCCGAGGGCT CTCAGTTCCA TTCATTCAAG AAGGTGCTCA TGGAGATGGG GCCACCGTAT 
TCCACGCAGC AGGAGCTTGC TTCTTTCCAC TCAGTCTCTA AGGGCTACAT GGGCGAGTGC 
GGGTTTCGTG GTGGCTATGT GGAGGTGGTA AACATGGATG CTGAGGTGCA GAAACAGATG 
GGGAAGCTGA TGAGTGTGCG GCTGTGTCCA CCAGTGCCAG GCCAGGCCTT GATGGACATG 
GTGGTCAGTC CGCCAACACC CTCCGAGCCG TCCTTCAAGC AGTTTCAAGC AGAGAGACAG 
GAGGTGCTGG CTGAACTGGC AGCCAAGGCT AAGCTCACGG AGCAGGTCTT CAATGAGGCT 
CCCGGGATCC GCTGCAACCC AGTGCAGGGC GCCATGTATT CCTTCCCTCA AGTGCAGCTG 
CCCTTGAAAG CGGTGCAGCG TGCTCAGGAA CTGGGCCTGG CCCCTGACAT GTTCTTCTGC 
CTGTGCCTCC TGGAAGAGAC TGGCATCTGC GTTGTGCCCG GGAGTGGCTT TGGGCAGCAG 
GAGGGCACCT ATCATTTCCG GATGACCATT CTGCCCCCCA TGGAGAAACT GCGGCTGCTG 
CTGGAAAAAC TCAGTCACTT CCATGCCAAG TTCACCCATG AGTACTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112301.1
CDS	114..1604
Translation	MASRVNDQSQASRNGLKGKVLTLDTMNPCVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFFRQVLALCVYPNLLSSPDFPEDAKRRAERILQACGGHSLGAYSISSGIQPIREDVAQYIERRDGGIPADPNNIFLSTGASDAIVTMLKLLVSGEGRARTGVLIPIPQYPLYSAALAELDAVQVDYYLDEERAWALDIAELRRALCQARDRCCPRVLCVINPGNPTGQVQTRECIEAVIRFAFKEGLFLMADEVYQDNVYAEGSQFHSFKKVLMEMGPPYSTQQELASFHSVSKGYMGECGFRGGYVEVVNMDAEVQKQMGKLMSVRLCPPVPGQALMDMVVSPPTPSEPSFKQFQAERQEVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPQVQLPLKAVQRAQELGLAPDMFFCLCLLEETGICVVPGSGFGQQEGTYHFRMTILPPMEKLRLLLEKLSHFHAKFTHEYS

Target ORF information:

RefSeq Version	NM_031039.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamic--pyruvic transaminase (Gpt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031039.1

ORF Insert Sequence:

ATGGCCTCAC GGGTGAATGA TCAAAGCCAG GCTTCAAGGA ATGGGCTGAA GGGAAAGGTG 
CTAACTCTGG ACACTATGAA CCCATGTGTG CGGAGGGTGG AGTATGCAGT TCGAGGACCC 
ATTGTGCAGC GTGCCTTGGA GCTGGAGCAG GAGCTGCGTC AGGGTGTGAA GAAGCCGTTT 
ACTGAGGTCA TCCGTGCCAA CATTGGGGAT GCACAAGCCA TGGGGCAGAG ACCCATCACC 
TTCTTCCGCC AGGTCCTGGC CCTCTGTGTC TACCCCAATC TTCTGAGCAG TCCTGACTTC 
CCAGAGGATG CCAAGAGAAG GGCAGAACGC ATCTTGCAGG CCTGCGGGGG CCACAGCCTG 
GGTGCCTATA GCATTAGCTC TGGAATCCAG CCGATCCGGG AGGATGTGGC GCAATACATT 
GAGAGAAGAG ACGGAGGCAT CCCCGCAGAC CCGAACAACA TATTTCTATC CACAGGGGCC 
AGCGATGCCA TCGTGACAAT GCTCAAGCTG CTGGTATCTG GCGAGGGCCG TGCACGAACA 
GGTGTACTCA TTCCCATTCC TCAGTACCCA CTGTACTCAG CCGCGCTGGC TGAACTGGAC 
GCCGTGCAAG TGGACTACTA CCTGGACGAA GAGCGCGCCT GGGCTCTGGA CATCGCAGAG 
CTGCGGCGCG CTCTGTGCCA GGCACGTGAC CGTTGCTGCC CTCGAGTACT GTGCGTCATC 
AACCCCGGCA ACCCCACTGG GCAGGTGCAG ACCCGTGAGT GCATCGAGGC CGTAATCCGC 
TTTGCTTTCA AAGAAGGACT CTTCTTGATG GCTGATGAGG TATACCAGGA CAACGTGTAT 
GCCGAGGGCT CTCAGTTCCA TTCATTCAAG AAGGTGCTCA TGGAGATGGG GCCACCGTAT 
TCCACGCAGC AGGAGCTTGC TTCTTTCCAC TCAGTCTCTA AGGGCTACAT GGGCGAGTGC 
GGGTTTCGTG GTGGCTATGT GGAGGTGGTA AACATGGATG CTGAGGTGCA GAAACAGATG 
GGGAAGCTGA TGAGTGTGCG GCTGTGTCCA CCAGTGCCAG GCCAGGCCTT GATGGACATG 
GTGGTCAGTC CGCCAACACC CTCCGAGCCG TCCTTCAAGC AGTTTCAAGC AGAGAGACAG 
GAGGTGCTGG CTGAACTGGC AGCCAAGGCT AAGCTCACGG AGCAGGTCTT CAATGAGGCT 
CCCGGGATCC GCTGCAACCC AGTGCAGGGC GCCATGTATT CCTTCCCTCA AGTGCAGCTG 
CCCTTGAAAG CGGTGCAGCG TGCTCAGGAA CTGGGCCTGG CCCCTGACAT GTTCTTCTGC 
CTGTGCCTCC TGGAAGAGAC TGGCATCTGC GTTGTGCCCG GGAGTGGCTT TGGGCAGCAG 
GAGGGCACCT ATCATTTCCG GATGACCATT CTGCCCCCCA TGGAGAAACT GCGGCTGCTG 
CTGGAAAAAC TCAGTCACTT CCATGCCAAG TTCACCCATG AGTACTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hepatitis B virus e antigen-negative chronic infection. Treatment based on glutamic pyruvic transaminase and hepatitis B virus deoxyribonucleic acid cut-off values.
Gastroenterologia y hepatologia(2017 Dec)
Guardiola-Ar?valo A,G?mez Rodr?guez R,Romero Guti?rrez M,G?mez Moreno AZ,Garc?a Vela A,S?nchez Sim?n R,G?mez Hernando C,Andr?s Esteban EM

Association between MFN2 gene polymorphisms and the risk and prognosis of acute liver failure: a case-control study in a Chinese population.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas50(6)e5758(2017 May)
Wei YL,Tian Q,Zhao XX,Qiu GZ,Xu Y

Effects of saikosaponins on hepatic damage induced by halothane and hypoxia in phenobarbital-pretreated rats.
Journal of anesthesia8(1)87-92(1994 Mar)
Nishiura T,Marukawa S,Ishida H,Orita M,Abe H

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Gpt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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