Cspg4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cspg4
Entrez Gene ID	81651
Full Name	chondroitin sulfate proteoglycan 4
Synonyms	Ng2
General protein information	Preferred Names chondroitin sulfate proteoglycan 4 Names chondroitin sulfate proteoglycan 4 HSN tumor-specific antigen chondroitin sulfate proteoglycan NG2 membrane-spanning proteoglycan NG2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24
Summary	extracellular matrix protein secreted by many cell types; member of the large chondroitin sulfate proteoglycan (CSPG) family; may be involved with angiogenesis -dependent processes such as wound healing or tumor growth [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031022.1	NP_112284.1	chondroitin sulfate proteoglycan 4 precursor

Pathways from BioSystems

Homologies

	CSPG4	XP_002804928.1
Canis lupus familiaris (dog)	CSPG4	XP_005638698.1
Bos taurus (cattle)	CSPG4	NP_001179711.1
Pan troglodytes (chimpanzee)	CSPG4	XP_001144835.3
Mus musculus (house mouse)	Cspg4	NP_620570.2
Homo sapiens (human)	CSPG4	NP_001888.2
Rattus norvegicus (Norway rat)	Cspg4	NP_112284.1
Gallus gallus (chicken)	CSPG4	XP_423277.4
Danio rerio (zebrafish)	cspg4	XP_001923457.2
Xenopus tropicalis (tropical clawed frog)	LOC101733782	XP_004916553.1

Gene Ontology
Bibliography

Related articles in PubMed

Distribution of type VI collagen in association with osteoblast lineages in the groove of Ranvier during rat postnatal development.
Kohara Y, Soeta S, Izu Y, Arai K, Amasaki H
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft20858-68(2016 Nov)

Roles of chondroitin sulfate proteoglycan 4 in fibrogenic/adipogenic differentiation in skeletal muscle tissues.
Takeuchi S, Nakano S, Nakamura K, Ozoe A, Chien P, Yoshihara H, Hakuno F, Matsuwaki T, Saeki Y, Takahashi S, Yamanouchi K, Nishihara M
Experimental cell research347(2)367-77(2016 10)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Entrapment via synaptic-like connections between NG2 proteoglycan+ cells and dystrophic axons in the lesion plays a role in regeneration failure after spinal cord injury.
Filous AR, Tran A, Howell CJ, Busch SA, Evans TA, Stallcup WB, Kang SH, Bergles DE, Lee SI, Levine JM, Silver J
The Journal of neuroscience : the official journal of the Society for Neuroscience34(49)16369-84(2014 Dec)

Paired related homeobox protein 1 is a regulator of stemness in adult neural stem/progenitor cells.
Shimozaki K, Clemenson GD Jr, Gage FH
The Journal of neuroscience : the official journal of the Society for Neuroscience33(9)4066-75(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings indicate that Col VI provided a characteristic microenvironment in the GOR and that NG2-Col VI interactions may regulate the differentiation of osteoblast lineages prior to terminal maturation.
Title: Distribution of type VI collagen in association with osteoblast lineages in the groove of Ranvier during rat postnatal development.

chondroitin sulfate proteoglycan 4 (CSPG4) is a potential mesenchymal progenitor cells marker.
Title: Roles of chondroitin sulfate proteoglycan 4 in fibrogenic/adipogenic differentiation in skeletal muscle tissues.

studies suggest that proteoglycan-mediated entrapment upon NG2+ cells is an additional obstacle to CNS axon regeneration.
Title: Entrapment via synaptic-like connections between NG2 proteoglycan+ cells and dystrophic axons in the lesion plays a role in regeneration failure after spinal cord injury.

our data suggested that NG2 proteoglycan expression and secretion hallmarked demarcation as a process that actively separated necrosis from vital tissue and therefore decisively impacts secondary neurodegeneration after ischemic stroke.
Title: NG2 and NG2-positive cells delineate focal cerebral infarct demarcation in rats.

NG2+ cells derived from the spinal cord following injury expressed high levels of laminin and fibronectin which promote neurite outgrowth.
Title: Adult NG2+ cells are permissive to neurite outgrowth and stabilize sensory axons during macrophage-induced axonal dieback after spinal cord injury.

The following Cspg4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cspg4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07405	NM_031022.1 Latest version!	Rattus norvegicus chondroitin sulfate proteoglycan 4 (Cspg4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1984.50 $2835.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cspg4 ( NM_031022.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112284.1 Rattus norvegicus chondroitin sulfate proteoglycan 4 (Cspg4), mRNA.

Price & Availability↑

CloneID	ORa07405
Clone ID Related Accession (Same CDS sequence)	NM_031022.1
Accession Version	NM_031022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	chondroitin sulfate proteoglycan 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X56541.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X56541.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689595, SAMEA2689596 [ECO:0000348] ##Evidence-Data-END##

ATGCTTCTCA GCCCGGGACA CCCGCTGTCA GCTCCAGCCC TGGCCTTGAT TCTTACCTTG 
GCCTTGTTGG TCAGATCTAC AGCTCCTGCC TCCTTCTTCG GGGAGAACCA CCTGGAGGTG 
CCTGTGCCCT CAGCCCTGAC CAGAGTAGAC TTACTGCTCC AGTTCTCCAC ATCGCAGCCC 
GAAGCCCTGC TCCTCTTGGC AGCAGGCCAA ACAGATCATC TCCTGCTGCA GCTCCAGTCT 
GGACACCTAC AGGTCAGACT TGCCCTGGGA CAAAATGAGC TGAGTCTGCA GACACCAGCA 
GACACGGTGC TGAGTGACTC CACAACCCAC ACCGTAGTGC TCACCGTCTC CAACAGCTGG 
GCTGTGCTGT CTGTTGATGG AGTGTTGAAC ACCTCTGCCC CCATCCCAAA AGCATCCCAC 
CTCAAAGTCC CCTATGGGCT CTTTGTGGGC TCCTCTGGAA GCCTTGACCT GCCTTACCTG 
AAGGGAATCA GTCGACCCCT GAGGGGCTGC CTCCACTCAG CCATTCTCAA TGGCCGCAAC 
CTTCTCCGCC CACTAACCCC CGATGTTCAT GAGGGTTGTG CTGAAGAATT CTCTGCTGGT 
GATGAAGTTG GCCTGGGCTT CTCTGGACCC CACTCACTGG CTGCCTTCCC TGCCTGGAGC 
ACGCGGGAGG AAGGCACCCT GGAGTTTACC CTCACCACTC GGAGTCAGCA AGCACCCCTG 
GCCTTCCAGG CCGGGGACAA GCGTGGCAAC TTTATCTACG TGGACATATT TGAGGGCCAC 
TTGCGGGCTG TGGTTGAAAA GGGTCAGGGT ACCATGCTGC TTCGTAACAG CGTGCCTGTG 
GCTGACGGGC AGCCCCATGA GGTCAGCGTA CACATAGATG TTCACCGGCT GGAAATCTCT 
GTAGATCAAT ACCCTACACG TACTTTCAAC CGTGGGGTCC TCAGCTACCT GGAGCCTCGT 
GGCAGTCTCC TCCTTGGGGG GCTCGATACA GAGGCCTCTC GCCACCTCCA AGAACACCGT 
CTGGGCCTGA CACCGGGGGC TGCCAACATC TCCCTGGTAG GCTGCATAGA AGATTTCAGT 
GTTAATGGCA GGAGGCTGGG CCTCCGGGAC GCCTGGCTGA CCCGTGACAT GGCAGCAGGC 
TGCAGGCCTG AGGAGGATGA GTATGAGGAA GAGGTCTATG GCCCGTTTGA AGCTTTCTCC 
ACCCTGGCGC CCGAAGCCTG GCCAGTCATG GATCTGCCAG AGCCGTGTGT TCCTGAGCCG 
GGACTGCCTG CCGTCTTCGC GAACTTCACG CAGCTGCTCA CCATTAGTCC CCTGGTCGTG 
GCCGAGGGTG GCACAGCCTG GCTTGAGTGG CGGCACGTGC AGCCCACGCT GGACCTGACA 
GAGGCGGAGC TGCGCAAGTC CCAGGTACTG TTCAGCGTGA GCCAGGGTGC ACGCCACGGC 
GAGCTGGAGC TAGACATCCC GGGAGCCCAA ACCCGAAAAA TGTTTACACT GTTGGACGTG 
GTGAACCGAA AGGCTCGCTT TGTTCACGAT GGCTCTGAAG ACACCTCTGA CCAGCTGATG 
CTGGAGGTAT CAGTGACTTC TCGGGCACCT GTGCCCTCTT GCCTGCGGAG GGGCCAAATT 
TACATTCTCC CCATCCAGGT AAACCCCGTC AACGACCCAC CTCGCATCGT CTTCCCGCAC 
GGCAGCCTCA TGGTGATCCT GGAGCACACA CAGAAGCCTC TGGGACCCGA GATTTTCCAG 
GCCTATGACC CAGACTCTGC TTGTGAGGGT CTCACCATCC AGCTTCTTGG TGTCTCCGCT 
AGTGTCCCTG TGGAACACCG AGACCAGCCC GGAGAGCCAG TGACTGAGTT TTCCTGTCGA 
GACCTAGAGG CAGGCAACAT AGTCTATGTC CACCGTGGCG GGCCTGCACA GGACCTGACA 
TTCCGGGTCA GTGATGGAAT GCAGGCCAGT GGGCCAGCTA CACTGAAGGT CGTGGCCGTC 
CGGCCAGCCA TACAGATCCT CCACAACACA GGGCTGCGCC TGGCCCAGGG CTCTGCTGCA 
GCCATCTTGC CTGCCAACCT GTCGGTAGAA ACGAATGCAG TAGGACAGGA TGTGAGCGTG 
CTGTTCCGAG TCACTGGCAC CTTGCAGTTT GGGGAGCTGC AGAAGCAGGG GGCCGGAGGG 
GTAGAGGGCA CCGAGTGGTG GGATACACTG GCCTTCCACC AGCGCGATGT GGAGCAAGGC 
CGAGTGAGGT ACCTGAGTAC TGACCCACAA CACCACACCC AAGACACAGT GGAGGACCTG 
ACCTTGGAGG TGCAGGTGGG CCAGGAGACA CTGAGCAATC TGTCTTTCCC AGTGACCATC 
CAGAGAGCCA CAGTATGGAT GCTGCAGCTT GAGCCTCTGC ATACACAGAA CCCTCATCAG 
GAAACCCTCA CCTCAGCCCA CCTAGAGGCT TCCCTGGAGG AGGAGGGAGA AGGAGGACCA 
TACCCTCATA TCTTCCACTA TGAGTTGGTT CAGGCCCCCA GGAGAGGCAA CCTCCTGCTC 
CAGGGTACAA GGCTGTCAGA TGGCCAGAGC TTCAGCCAGA GTGACCTGCA GGCCGGTCGT 
GTGACCTACA GGGCCACAAC GCGGACCTCT GAGGCAGCTG AGGACTCCTT CCGTTTCCGG 
GTCACATCTC CACCACATTT CTCCCCGCTC TACACCTTCC CTATCCACAT TGGCGGTGAC 
CCAAACGCTC CTGTCCTCAC TAACGTCCTG CTCATGGTAC CCGAGGGAGG GGAGGGAGTG 
CTGTCTGCTG ACCACCTCTT TGTCAAGAGT CTCAACAGTG CCAGCTATCT CTATGAGGTT 
ATGGAGCAGC CCCACCATGG CAGCTTGGCT TGGAGGGACC CAAAAGGTAG AGCCACCCCA 
GTAACATCCT TTACTAATGA GGACCTGCTA CACGGCCGAC TGGTCTACCA GCACGATGAC 
TCTGAGACCA TAGAGGATGA TATCCCATTT GTGGCCACAC GCCAGGGCGA GGGCAGCGGT 
GACATGGCCT GGGAGGAGGT GCGTGGTGTC TTCCGAGTGG CCATCCAGCC TGTGAACGAT 
CACGCCCCTG TGCAGACCAT CAGCCGTGTC TTCCACGTGG CCCGGGGCGG ACAGCGGCTG 
TTGACTACAG ATGATGTGGC CTTCAGTGAT GCTGATTCGG GCTTCAGTGA CGCGCAACTG 
GTGCTGACCC GCAAGGACCT CCTCTTTGGC AGCATCGTGG CTATGGAGGA GCCCACGAGG 
CCCATCTACC GTTTCACCCA AGAGGATCTC AGGAAGAAGC AAGTCCTGTT TGTGCACTCA 
GGGGCCGACC ATGGCTGGCT CCAGCTACAG GTGTCTGATG GGCAACACCA GGCTACTGCC 
ATGCTGGAGG TGCAGGCCTC AGAGCCCTAT CTCCACGTAG CCAATAGTTC CAGTCTTGTG 
GTTCCTCAAG GAGGCCAGGG CACCATCGAC ACAGCCGTGC TCCACCTGGA CACCAACCTA 
GATATACGAA GTGGGAATGA GGTCCACTAC CATGTCACAG CTGGCCCTCA CTGGGGACAG 
CTGCTCCGGG ATGGCCAGTC AGTCACCTCC TTCTCGCAAC GGGACTTGCT GGATGGGGCC 
ATTCTCTACA GCCACAATGG CAGCCTCAGC CCGCAAGACA CCCTGGCCCT TTCTGTGGCA 
GCAGGGCCAG TACACACTAG CACCGTCCTA CAAGTGACCA TTGCCCTAGA GGGCCCCCTG 
GCTCCACTAC AACTGGTGCA GCACAAAAGG ATCTATGTCT TCCAAGGGGA GGCAGCTGAG 
ATCAGAAGGG ACCAGCTAGA GGTAGTCCAG GAGGCAGTGC TGCCTGCCGA CATCATGTTC 
TCGTTGAGAA GCCCCCCGAA CGCTGGCTAC TTGGTGATGG TGTCCCACGG TGCTTCAGCA 
GATGGGCCAC CCAGCCTGGA CCCTGTGCAG CGCTTCTCCC AAGAGGCAAT AAATTCAGGC 
CGGGTTCTCT ACTTGCACTC TCGCCCTGGA GCCTGGAGTG ATTCCTTCTC CCTGGATGTG 
GCCTCGGGTC TGGGCGATCC TCTCGAAGGC ATCTCTGTGG AGCTGGAAGT GTTGCCCACC 
GTCATCCCCC TGGATGTTCA AAACTTCAGC GTTCCTGAGG GTGGCACTCG TACGCTGGCC 
CCTCCGCTGA TCCAAATCAC TGGGCCCTAC TTGGGGACAC TGCCAGGCCT TGTCCTACAG 
GTGCTAGAGC CACCGCAGCA CGGGGCCCTG CAGAAGGAGG ACCGTCCTCA AGATGGGACC 
CTCAGCACTT TCTCCTGGAG AGAGGTGGAA GAGCAGCTGA TCCGATACGT GCATGATGGG 
AGTGAGACGC AGACGGACGG CTTCATCCTG CTAGCTAATG CCTCAGAGAT GGATCGCCAG 
AGCCAGCCCA TGGCCTTCAC TATCACCATC CTCCCTGTTA ATGACCAACC CCCTGTCATC 
ACCACAAACA CAGGCTTGCA GATCTGGGAG GGGGCTATTG TGCCCATCCC TCCTGAGGCC 
CTCAGGGGAA TAGACAGTGA TTCAGGGCCG GAAGACTTGG TCTACACCAT CGAGCAGCCC 
AGCAATGGAC GGATAGCCTT GAGGGTAGCA CCAGACGCCG AGGCCCACCG CTTCACACAG 
GCCCAGCTGG ACAGCGGGCT TGTGCTGTTC TCACACAGAG GAGCCCTGGA AGGAGGCTTC 
CACTTCGACC TCTCTGATGG CGTACACACT TCTCCTGGAC ATTTCTTCCG AGTGGTAGCC 
CAGAAGCAGG TACTCCTCTC CTTGGAGGGC AGCCGGAAGC TGACTGTCTG TCCGGAGTCT 
GTGCAGCCAC TCAGCAGCCA GAGCTTGAGT GCCAGCTCCA GTACAGGCTC CGACCCTCGT 
CACCTGCTCT ACCAGGTGGT AAGAGGCCCC CAGCTCGGCC GACTCCTCCA TGCCCAGCAA 
GGAAGTGCAG AGGAGGCTTT GGTGAACTTC ACCCAGGCTG AGGTGAATGC TGGGAATATT 
CTGTATGAGC ATGAGATATC CTCTGAGCCC TTCTGGGAAG CCCACGATAC CATTGGTCTC 
CTGCTGTCTT CATCACCTGC GAGGGATCTG GCTGCCACCC TGGCTGTGAC TGTGTCTTTC 
GACGCTGCCT GTCCCCAGCG GCCTAGCCGT CTCTGGAGGA ACAAAGGTCT TTGGGTCCCT 
GAGGGCCAGC GGGCCAAGAT CACTGTGGCT GCCCTTGATG CCGCCAACCT CCTAGCCAGT 
GTGCCAGCAT CTCAGCGCGG TCGGCATGAT GTACTCTTCC AGATCACACA GTTCCCCACC 
CGGGGTCAGC TCCTGGTGTC CGAGGAGCCA CTCCATGCCA GGAGACCCCA CTTCCTGCAA 
TCTGAGCTGA CTGCAGGACA GCTGGTGTAT GCCCACGGTG GTGGGGGCAC CCAGCAGGAT 
GGCTTCCGCT TCCGTGCCCA CCTCCAGGGA CCAACGGGAG CTTCCGTGGC AGGACCCCAA 
ACCTCTGAGG CTTTTGTCAT CACTGTGAGG GACGTGAATG AGCGACCCCC TCAGCCACAG 
GCCTCCATTC CTCTCCGCAT CACCAGGGGC TCACGAGCCC CTGTCTCTCG AGCCCAGCTG 
AGTGTCGTAG ACCCAGACTC AGCTCCAGGG GAAATTGAGT ACGAAGTACA GAGGGCACCC 
CACAATGGCT TCCTGAGCCT GGCTGGGGAC AACACTGGGC CTGTGACTCA CTTTACACAG 
GCTGATGTGG ATGCAGGGCG ACTGGCCTTT GTGGCAAATG GGAGCAGTGT GGCTGGCGTC 
TTCCAGTTGA GCATGTCTGA TGGAGCCAGC CCCCCAATAC CCATGTCGCT GGCTGTGGAT 
GTCTTGCCAT CCACCATTGA GGTGCAGCTG CGAGCGCCCC TGGAGGTGCC TCAAGCCCTA 
GGACGTTCCT CACTGAGCAG GCAGCAGCTC CAGGTGATTT CAGATCGTGA GGAGCCAGAT 
GTAGCTTACC GCCTCACTCA GGGGCCCCTG TATGGGCAGG TACTAGTGGG GGGGCAGCCC 
GCTTCAGCCT TCAGCCAGCT GCAGGTAGAC CAGGGGGACG TGGTCTTTGC CTTCACCAAC 
TTTTCTTCCT CTCAGGATCA TTTCAAAGTC CTGGCTCTTG CTAGGGGTGT GAATGCATCA 
GCTACTGTAA ATGTCACAGT GCAGGCCCTG TTGCATGTGT GGGCCGGTGG GCCATGGCCT 
CAGGGTACCA CCTTGCGCCT TGACCCCACT GTGCTCGATG CCAGTGAACT GGCCAACCGC 
ACAGGCAGTA TGCCCCGCTT CCGGCTCCTG GAAGGACCCC GGTACGGCCG TGTGGTTCGT 
GTGTCCCAAG GCCGAGCAGA ATCTAGGACC AACCAACTTG TGGAAGATTT CACACAGCAA 
GACCTGGAAG AGGGAAGGCT GGGGCTAGAG GTGGGCAGGC CAGAGGGCAG GTCCACTGGC 
CCAACAGGTG ATAGACTTAC CCTGGAGCTG CAGGCAACGG GTGTCCCACC TGCTGTGGCC 
TTGCTGGACT TTGCCACTGA GCCTTACCAT GCAGCCAAGT TCTATAAGGT GACCCTACTC 
AGTGTCCCTG AGGCTGCTCG TACAGAAACA GAGAAAACAG GAAAAAGCAC CCCCACTGGC 
CAGCCAGGCC AAGCAGCATC TAGCCCCATG CCCACTGTAG CCAAAAGTGG TTTCTTGGGC 
TTCTTAGAGG CCAACATGTT CAGCGTCATC ATCCCCGTGT GCCTGGTCCT CCTGCTCCTG 
GCTCTCATCT TGCCTCTGCT TTTCTACCTC CGCAAACGCA ACAAGACAGG TAAGCATGAT 
GTCCAGGTGT TGACCGCCAA ACCCCGCAAT GGCCTAGCTG GTGACACAGA GACCTTTCGA 
AAGGTGGAGC CAGGCCAAGC CATTCCACTC ACAACTGTAC CTGGCCAGGG GCCCCCACCC 
GGAGGCCAGC CTGACCCAGA ACTATTACAG TTTTGCCGGA CACCCAATCC TGCCCTCAGG 
AATGGCCAGT ACTGGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112284.1
CDS	70..7050
Translation	MLLSPGHPLSAPALALILTLALLVRSTAPASFFGENHLEVPVPSALTRVDLLLQFSTSQPEALLLLAAGQTDHLLLQLQSGHLQVRLALGQNELSLQTPADTVLSDSTTHTVVLTVSNSWAVLSVDGVLNTSAPIPKASHLKVPYGLFVGSSGSLDLPYLKGISRPLRGCLHSAILNGRNLLRPLTPDVHEGCAEEFSAGDEVGLGFSGPHSLAAFPAWSTREEGTLEFTLTTRSQQAPLAFQAGDKRGNFIYVDIFEGHLRAVVEKGQGTMLLRNSVPVADGQPHEVSVHIDVHRLEISVDQYPTRTFNRGVLSYLEPRGSLLLGGLDTEASRHLQEHRLGLTPGAANISLVGCIEDFSVNGRRLGLRDAWLTRDMAAGCRPEEDEYEEEVYGPFEAFSTLAPEAWPVMDLPEPCVPEPGLPAVFANFTQLLTISPLVVAEGGTAWLEWRHVQPTLDLTEAELRKSQVLFSVSQGARHGELELDIPGAQTRKMFTLLDVVNRKARFVHDGSEDTSDQLMLEVSVTSRAPVPSCLRRGQIYILPIQVNPVNDPPRIVFPHGSLMVILEHTQKPLGPEIFQAYDPDSACEGLTIQLLGVSASVPVEHRDQPGEPVTEFSCRDLEAGNIVYVHRGGPAQDLTFRVSDGMQASGPATLKVVAVRPAIQILHNTGLRLAQGSAAAILPANLSVETNAVGQDVSVLFRVTGTLQFGELQKQGAGGVEGTEWWDTLAFHQRDVEQGRVRYLSTDPQHHTQDTVEDLTLEVQVGQETLSNLSFPVTIQRATVWMLQLEPLHTQNPHQETLTSAHLEASLEEEGEGGPYPHIFHYELVQAPRRGNLLLQGTRLSDGQSFSQSDLQAGRVTYRATTRTSEAAEDSFRFRVTSPPHFSPLYTFPIHIGGDPNAPVLTNVLLMVPEGGEGVLSADHLFVKSLNSASYLYEVMEQPHHGSLAWRDPKGRATPVTSFTNEDLLHGRLVYQHDDSETIEDDIPFVATRQGEGSGDMAWEEVRGVFRVAIQPVNDHAPVQTISRVFHVARGGQRLLTTDDVAFSDADSGFSDAQLVLTRKDLLFGSIVAMEEPTRPIYRFTQEDLRKKQVLFVHSGADHGWLQLQVSDGQHQATAMLEVQASEPYLHVANSSSLVVPQGGQGTIDTAVLHLDTNLDIRSGNEVHYHVTAGPHWGQLLRDGQSVTSFSQRDLLDGAILYSHNGSLSPQDTLALSVAAGPVHTSTVLQVTIALEGPLAPLQLVQHKRIYVFQGEAAEIRRDQLEVVQEAVLPADIMFSLRSPPNAGYLVMVSHGASADGPPSLDPVQRFSQEAINSGRVLYLHSRPGAWSDSFSLDVASGLGDPLEGISVELEVLPTVIPLDVQNFSVPEGGTRTLAPPLIQITGPYLGTLPGLVLQVLEPPQHGALQKEDRPQDGTLSTFSWREVEEQLIRYVHDGSETQTDGFILLANASEMDRQSQPMAFTITILPVNDQPPVITTNTGLQIWEGAIVPIPPEALRGIDSDSGPEDLVYTIEQPSNGRIALRVAPDAEAHRFTQAQLDSGLVLFSHRGALEGGFHFDLSDGVHTSPGHFFRVVAQKQVLLSLEGSRKLTVCPESVQPLSSQSLSASSSTGSDPRHLLYQVVRGPQLGRLLHAQQGSAEEALVNFTQAEVNAGNILYEHEISSEPFWEAHDTIGLLLSSSPARDLAATLAVTVSFDAACPQRPSRLWRNKGLWVPEGQRAKITVAALDAANLLASVPASQRGRHDVLFQITQFPTRGQLLVSEEPLHARRPHFLQSELTAGQLVYAHGGGGTQQDGFRFRAHLQGPTGASVAGPQTSEAFVITVRDVNERPPQPQASIPLRITRGSRAPVSRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLAGDNTGPVTHFTQADVDAGRLAFVANGSSVAGVFQLSMSDGASPPIPMSLAVDVLPSTIEVQLRAPLEVPQALGRSSLSRQQLQVISDREEPDVAYRLTQGPLYGQVLVGGQPASAFSQLQVDQGDVVFAFTNFSSSQDHFKVLALARGVNASATVNVTVQALLHVWAGGPWPQGTTLRLDPTVLDASELANRTGSMPRFRLLEGPRYGRVVRVSQGRAESRTNQLVEDFTQQDLEEGRLGLEVGRPEGRSTGPTGDRLTLELQATGVPPAVALLDFATEPYHAAKFYKVTLLSVPEAARTETEKTGKSTPTGQPGQAASSPMPTVAKSGFLGFLEANMFSVIIPVCLVLLLLALILPLLFYLRKRNKTGKHDVQVLTAKPRNGLAGDTETFRKVEPGQAIPLTTVPGQGPPPGGQPDPELLQFCRTPNPALRNGQYWV

Target ORF information:

RefSeq Version	NM_031022.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chondroitin sulfate proteoglycan 4 (Cspg4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031022.1

ORF Insert Sequence:

ATGCTTCTCA GCCCGGGACA CCCGCTGTCA GCTCCAGCCC TGGCCTTGAT TCTTACCTTG 
GCCTTGTTGG TCAGATCTAC AGCTCCTGCC TCCTTCTTCG GGGAGAACCA CCTGGAGGTG 
CCTGTGCCCT CAGCCCTGAC CAGAGTAGAC TTACTGCTCC AGTTCTCCAC ATCGCAGCCC 
GAAGCCCTGC TCCTCTTGGC AGCAGGCCAA ACAGATCATC TCCTGCTGCA GCTCCAGTCT 
GGACACCTAC AGGTCAGACT TGCCCTGGGA CAAAATGAGC TGAGTCTGCA GACACCAGCA 
GACACGGTGC TGAGTGACTC CACAACCCAC ACCGTAGTGC TCACCGTCTC CAACAGCTGG 
GCTGTGCTGT CTGTTGATGG AGTGTTGAAC ACCTCTGCCC CCATCCCAAA AGCATCCCAC 
CTCAAAGTCC CCTATGGGCT CTTTGTGGGC TCCTCTGGAA GCCTTGACCT GCCTTACCTG 
AAGGGAATCA GTCGACCCCT GAGGGGCTGC CTCCACTCAG CCATTCTCAA TGGCCGCAAC 
CTTCTCCGCC CACTAACCCC CGATGTTCAT GAGGGTTGTG CTGAAGAATT CTCTGCTGGT 
GATGAAGTTG GCCTGGGCTT CTCTGGACCC CACTCACTGG CTGCCTTCCC TGCCTGGAGC 
ACGCGGGAGG AAGGCACCCT GGAGTTTACC CTCACCACTC GGAGTCAGCA AGCACCCCTG 
GCCTTCCAGG CCGGGGACAA GCGTGGCAAC TTTATCTACG TGGACATATT TGAGGGCCAC 
TTGCGGGCTG TGGTTGAAAA GGGTCAGGGT ACCATGCTGC TTCGTAACAG CGTGCCTGTG 
GCTGACGGGC AGCCCCATGA GGTCAGCGTA CACATAGATG TTCACCGGCT GGAAATCTCT 
GTAGATCAAT ACCCTACACG TACTTTCAAC CGTGGGGTCC TCAGCTACCT GGAGCCTCGT 
GGCAGTCTCC TCCTTGGGGG GCTCGATACA GAGGCCTCTC GCCACCTCCA AGAACACCGT 
CTGGGCCTGA CACCGGGGGC TGCCAACATC TCCCTGGTAG GCTGCATAGA AGATTTCAGT 
GTTAATGGCA GGAGGCTGGG CCTCCGGGAC GCCTGGCTGA CCCGTGACAT GGCAGCAGGC 
TGCAGGCCTG AGGAGGATGA GTATGAGGAA GAGGTCTATG GCCCGTTTGA AGCTTTCTCC 
ACCCTGGCGC CCGAAGCCTG GCCAGTCATG GATCTGCCAG AGCCGTGTGT TCCTGAGCCG 
GGACTGCCTG CCGTCTTCGC GAACTTCACG CAGCTGCTCA CCATTAGTCC CCTGGTCGTG 
GCCGAGGGTG GCACAGCCTG GCTTGAGTGG CGGCACGTGC AGCCCACGCT GGACCTGACA 
GAGGCGGAGC TGCGCAAGTC CCAGGTACTG TTCAGCGTGA GCCAGGGTGC ACGCCACGGC 
GAGCTGGAGC TAGACATCCC GGGAGCCCAA ACCCGAAAAA TGTTTACACT GTTGGACGTG 
GTGAACCGAA AGGCTCGCTT TGTTCACGAT GGCTCTGAAG ACACCTCTGA CCAGCTGATG 
CTGGAGGTAT CAGTGACTTC TCGGGCACCT GTGCCCTCTT GCCTGCGGAG GGGCCAAATT 
TACATTCTCC CCATCCAGGT AAACCCCGTC AACGACCCAC CTCGCATCGT CTTCCCGCAC 
GGCAGCCTCA TGGTGATCCT GGAGCACACA CAGAAGCCTC TGGGACCCGA GATTTTCCAG 
GCCTATGACC CAGACTCTGC TTGTGAGGGT CTCACCATCC AGCTTCTTGG TGTCTCCGCT 
AGTGTCCCTG TGGAACACCG AGACCAGCCC GGAGAGCCAG TGACTGAGTT TTCCTGTCGA 
GACCTAGAGG CAGGCAACAT AGTCTATGTC CACCGTGGCG GGCCTGCACA GGACCTGACA 
TTCCGGGTCA GTGATGGAAT GCAGGCCAGT GGGCCAGCTA CACTGAAGGT CGTGGCCGTC 
CGGCCAGCCA TACAGATCCT CCACAACACA GGGCTGCGCC TGGCCCAGGG CTCTGCTGCA 
GCCATCTTGC CTGCCAACCT GTCGGTAGAA ACGAATGCAG TAGGACAGGA TGTGAGCGTG 
CTGTTCCGAG TCACTGGCAC CTTGCAGTTT GGGGAGCTGC AGAAGCAGGG GGCCGGAGGG 
GTAGAGGGCA CCGAGTGGTG GGATACACTG GCCTTCCACC AGCGCGATGT GGAGCAAGGC 
CGAGTGAGGT ACCTGAGTAC TGACCCACAA CACCACACCC AAGACACAGT GGAGGACCTG 
ACCTTGGAGG TGCAGGTGGG CCAGGAGACA CTGAGCAATC TGTCTTTCCC AGTGACCATC 
CAGAGAGCCA CAGTATGGAT GCTGCAGCTT GAGCCTCTGC ATACACAGAA CCCTCATCAG 
GAAACCCTCA CCTCAGCCCA CCTAGAGGCT TCCCTGGAGG AGGAGGGAGA AGGAGGACCA 
TACCCTCATA TCTTCCACTA TGAGTTGGTT CAGGCCCCCA GGAGAGGCAA CCTCCTGCTC 
CAGGGTACAA GGCTGTCAGA TGGCCAGAGC TTCAGCCAGA GTGACCTGCA GGCCGGTCGT 
GTGACCTACA GGGCCACAAC GCGGACCTCT GAGGCAGCTG AGGACTCCTT CCGTTTCCGG 
GTCACATCTC CACCACATTT CTCCCCGCTC TACACCTTCC CTATCCACAT TGGCGGTGAC 
CCAAACGCTC CTGTCCTCAC TAACGTCCTG CTCATGGTAC CCGAGGGAGG GGAGGGAGTG 
CTGTCTGCTG ACCACCTCTT TGTCAAGAGT CTCAACAGTG CCAGCTATCT CTATGAGGTT 
ATGGAGCAGC CCCACCATGG CAGCTTGGCT TGGAGGGACC CAAAAGGTAG AGCCACCCCA 
GTAACATCCT TTACTAATGA GGACCTGCTA CACGGCCGAC TGGTCTACCA GCACGATGAC 
TCTGAGACCA TAGAGGATGA TATCCCATTT GTGGCCACAC GCCAGGGCGA GGGCAGCGGT 
GACATGGCCT GGGAGGAGGT GCGTGGTGTC TTCCGAGTGG CCATCCAGCC TGTGAACGAT 
CACGCCCCTG TGCAGACCAT CAGCCGTGTC TTCCACGTGG CCCGGGGCGG ACAGCGGCTG 
TTGACTACAG ATGATGTGGC CTTCAGTGAT GCTGATTCGG GCTTCAGTGA CGCGCAACTG 
GTGCTGACCC GCAAGGACCT CCTCTTTGGC AGCATCGTGG CTATGGAGGA GCCCACGAGG 
CCCATCTACC GTTTCACCCA AGAGGATCTC AGGAAGAAGC AAGTCCTGTT TGTGCACTCA 
GGGGCCGACC ATGGCTGGCT CCAGCTACAG GTGTCTGATG GGCAACACCA GGCTACTGCC 
ATGCTGGAGG TGCAGGCCTC AGAGCCCTAT CTCCACGTAG CCAATAGTTC CAGTCTTGTG 
GTTCCTCAAG GAGGCCAGGG CACCATCGAC ACAGCCGTGC TCCACCTGGA CACCAACCTA 
GATATACGAA GTGGGAATGA GGTCCACTAC CATGTCACAG CTGGCCCTCA CTGGGGACAG 
CTGCTCCGGG ATGGCCAGTC AGTCACCTCC TTCTCGCAAC GGGACTTGCT GGATGGGGCC 
ATTCTCTACA GCCACAATGG CAGCCTCAGC CCGCAAGACA CCCTGGCCCT TTCTGTGGCA 
GCAGGGCCAG TACACACTAG CACCGTCCTA CAAGTGACCA TTGCCCTAGA GGGCCCCCTG 
GCTCCACTAC AACTGGTGCA GCACAAAAGG ATCTATGTCT TCCAAGGGGA GGCAGCTGAG 
ATCAGAAGGG ACCAGCTAGA GGTAGTCCAG GAGGCAGTGC TGCCTGCCGA CATCATGTTC 
TCGTTGAGAA GCCCCCCGAA CGCTGGCTAC TTGGTGATGG TGTCCCACGG TGCTTCAGCA 
GATGGGCCAC CCAGCCTGGA CCCTGTGCAG CGCTTCTCCC AAGAGGCAAT AAATTCAGGC 
CGGGTTCTCT ACTTGCACTC TCGCCCTGGA GCCTGGAGTG ATTCCTTCTC CCTGGATGTG 
GCCTCGGGTC TGGGCGATCC TCTCGAAGGC ATCTCTGTGG AGCTGGAAGT GTTGCCCACC 
GTCATCCCCC TGGATGTTCA AAACTTCAGC GTTCCTGAGG GTGGCACTCG TACGCTGGCC 
CCTCCGCTGA TCCAAATCAC TGGGCCCTAC TTGGGGACAC TGCCAGGCCT TGTCCTACAG 
GTGCTAGAGC CACCGCAGCA CGGGGCCCTG CAGAAGGAGG ACCGTCCTCA AGATGGGACC 
CTCAGCACTT TCTCCTGGAG AGAGGTGGAA GAGCAGCTGA TCCGATACGT GCATGATGGG 
AGTGAGACGC AGACGGACGG CTTCATCCTG CTAGCTAATG CCTCAGAGAT GGATCGCCAG 
AGCCAGCCCA TGGCCTTCAC TATCACCATC CTCCCTGTTA ATGACCAACC CCCTGTCATC 
ACCACAAACA CAGGCTTGCA GATCTGGGAG GGGGCTATTG TGCCCATCCC TCCTGAGGCC 
CTCAGGGGAA TAGACAGTGA TTCAGGGCCG GAAGACTTGG TCTACACCAT CGAGCAGCCC 
AGCAATGGAC GGATAGCCTT GAGGGTAGCA CCAGACGCCG AGGCCCACCG CTTCACACAG 
GCCCAGCTGG ACAGCGGGCT TGTGCTGTTC TCACACAGAG GAGCCCTGGA AGGAGGCTTC 
CACTTCGACC TCTCTGATGG CGTACACACT TCTCCTGGAC ATTTCTTCCG AGTGGTAGCC 
CAGAAGCAGG TACTCCTCTC CTTGGAGGGC AGCCGGAAGC TGACTGTCTG TCCGGAGTCT 
GTGCAGCCAC TCAGCAGCCA GAGCTTGAGT GCCAGCTCCA GTACAGGCTC CGACCCTCGT 
CACCTGCTCT ACCAGGTGGT AAGAGGCCCC CAGCTCGGCC GACTCCTCCA TGCCCAGCAA 
GGAAGTGCAG AGGAGGCTTT GGTGAACTTC ACCCAGGCTG AGGTGAATGC TGGGAATATT 
CTGTATGAGC ATGAGATATC CTCTGAGCCC TTCTGGGAAG CCCACGATAC CATTGGTCTC 
CTGCTGTCTT CATCACCTGC GAGGGATCTG GCTGCCACCC TGGCTGTGAC TGTGTCTTTC 
GACGCTGCCT GTCCCCAGCG GCCTAGCCGT CTCTGGAGGA ACAAAGGTCT TTGGGTCCCT 
GAGGGCCAGC GGGCCAAGAT CACTGTGGCT GCCCTTGATG CCGCCAACCT CCTAGCCAGT 
GTGCCAGCAT CTCAGCGCGG TCGGCATGAT GTACTCTTCC AGATCACACA GTTCCCCACC 
CGGGGTCAGC TCCTGGTGTC CGAGGAGCCA CTCCATGCCA GGAGACCCCA CTTCCTGCAA 
TCTGAGCTGA CTGCAGGACA GCTGGTGTAT GCCCACGGTG GTGGGGGCAC CCAGCAGGAT 
GGCTTCCGCT TCCGTGCCCA CCTCCAGGGA CCAACGGGAG CTTCCGTGGC AGGACCCCAA 
ACCTCTGAGG CTTTTGTCAT CACTGTGAGG GACGTGAATG AGCGACCCCC TCAGCCACAG 
GCCTCCATTC CTCTCCGCAT CACCAGGGGC TCACGAGCCC CTGTCTCTCG AGCCCAGCTG 
AGTGTCGTAG ACCCAGACTC AGCTCCAGGG GAAATTGAGT ACGAAGTACA GAGGGCACCC 
CACAATGGCT TCCTGAGCCT GGCTGGGGAC AACACTGGGC CTGTGACTCA CTTTACACAG 
GCTGATGTGG ATGCAGGGCG ACTGGCCTTT GTGGCAAATG GGAGCAGTGT GGCTGGCGTC 
TTCCAGTTGA GCATGTCTGA TGGAGCCAGC CCCCCAATAC CCATGTCGCT GGCTGTGGAT 
GTCTTGCCAT CCACCATTGA GGTGCAGCTG CGAGCGCCCC TGGAGGTGCC TCAAGCCCTA 
GGACGTTCCT CACTGAGCAG GCAGCAGCTC CAGGTGATTT CAGATCGTGA GGAGCCAGAT 
GTAGCTTACC GCCTCACTCA GGGGCCCCTG TATGGGCAGG TACTAGTGGG GGGGCAGCCC 
GCTTCAGCCT TCAGCCAGCT GCAGGTAGAC CAGGGGGACG TGGTCTTTGC CTTCACCAAC 
TTTTCTTCCT CTCAGGATCA TTTCAAAGTC CTGGCTCTTG CTAGGGGTGT GAATGCATCA 
GCTACTGTAA ATGTCACAGT GCAGGCCCTG TTGCATGTGT GGGCCGGTGG GCCATGGCCT 
CAGGGTACCA CCTTGCGCCT TGACCCCACT GTGCTCGATG CCAGTGAACT GGCCAACCGC 
ACAGGCAGTA TGCCCCGCTT CCGGCTCCTG GAAGGACCCC GGTACGGCCG TGTGGTTCGT 
GTGTCCCAAG GCCGAGCAGA ATCTAGGACC AACCAACTTG TGGAAGATTT CACACAGCAA 
GACCTGGAAG AGGGAAGGCT GGGGCTAGAG GTGGGCAGGC CAGAGGGCAG GTCCACTGGC 
CCAACAGGTG ATAGACTTAC CCTGGAGCTG CAGGCAACGG GTGTCCCACC TGCTGTGGCC 
TTGCTGGACT TTGCCACTGA GCCTTACCAT GCAGCCAAGT TCTATAAGGT GACCCTACTC 
AGTGTCCCTG AGGCTGCTCG TACAGAAACA GAGAAAACAG GAAAAAGCAC CCCCACTGGC 
CAGCCAGGCC AAGCAGCATC TAGCCCCATG CCCACTGTAG CCAAAAGTGG TTTCTTGGGC 
TTCTTAGAGG CCAACATGTT CAGCGTCATC ATCCCCGTGT GCCTGGTCCT CCTGCTCCTG 
GCTCTCATCT TGCCTCTGCT TTTCTACCTC CGCAAACGCA ACAAGACAGG TAAGCATGAT 
GTCCAGGTGT TGACCGCCAA ACCCCGCAAT GGCCTAGCTG GTGACACAGA GACCTTTCGA 
AAGGTGGAGC CAGGCCAAGC CATTCCACTC ACAACTGTAC CTGGCCAGGG GCCCCCACCC 
GGAGGCCAGC CTGACCCAGA ACTATTACAG TTTTGCCGGA CACCCAATCC TGCCCTCAGG 
AATGGCCAGT ACTGGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distribution of type VI collagen in association with osteoblast lineages in the groove of Ranvier during rat postnatal development.
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft20858-68(2016 Nov)
Kohara Y,Soeta S,Izu Y,Arai K,Amasaki H

Roles of chondroitin sulfate proteoglycan 4 in fibrogenic/adipogenic differentiation in skeletal muscle tissues.
Experimental cell research347(2)367-77(2016 10)
Takeuchi S,Nakano S,Nakamura K,Ozoe A,Chien P,Yoshihara H,Hakuno F,Matsuwaki T,Saeki Y,Takahashi S,Yamanouchi K,Nishihara M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Entrapment via synaptic-like connections between NG2 proteoglycan+ cells and dystrophic axons in the lesion plays a role in regeneration failure after spinal cord injury.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(49)16369-84(2014 Dec)
Filous AR,Tran A,Howell CJ,Busch SA,Evans TA,Stallcup WB,Kang SH,Bergles DE,Lee SI,Levine JM,Silver J

Paired related homeobox protein 1 is a regulator of stemness in adult neural stem/progenitor cells.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(9)4066-75(2013 Feb)
Shimozaki K,Clemenson GD Jr,Gage FH

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Cspg4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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