Chrm2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrm2
Entrez Gene ID	81645
Full Name	cholinergic receptor, muscarinic 2
Synonyms	Acm2
General protein information	Preferred Names cholinergic receptor, muscarinic 2 Names muscarinic acetylcholine receptor M2 7TM receptor M2 muscarinic acetylcholine receptor cholinergic receptor, muscarinic 2, cardiac muscarinic acetylcholine receptor M2-like muscarinic receptor m2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q22
Summary	may inhibit cholinergic-mediated bronchoconstriction; may regulate bladder contraction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031016.1	NP_112278.1	muscarinic acetylcholine receptor M2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

M2 muscarinic receptor activation inhibits cell proliferation and migration of rat adipose-mesenchymal stem cells.
Piovesana R, Melfi S, Fiore M, Magnaghi V, Tata AM
Journal of cellular physiology(2017 Dec)

Changes in Muscarinic M2 Receptor Levels in the Cortex of Subjects with Bipolar Disorder and Major Depressive Disorder and in Rats after Treatment with Mood Stabilisers and Antidepressants.
Gibbons AS, Jeon WJ, Scarr E, Dean B
The international journal of neuropsychopharmacology19(4)(2016 Apr)

Laser-capture microdissection for analysis of cell type-specific gene expression of muscarinic receptor subtypes in the rat bladder with cyclophosphamide-induced cystitis.
Sugino Y, O'Malley KJ, Wang Z, Tyagi P, Birder LA, Ogawa O, Yoshimura N
International urology and nephrology47(4)637-42(2015 Apr)

Cortical cholinergic dysregulation as a long-term consequence of neonatal hypoglycemia.
Anju TR, Paulose CS
Biochemistry and cell biology = Biochimie et biologie cellulaire93(1)47-53(2015 Feb)

Mitochondrial Ultrastructural Alterations and Declined M2 Receptor Density Were Involved in Cardiac Dysfunction in Rats after Long Term Treatment with Autoantibodies against M2 Muscarinic Receptor.
Zhang S, He Z, Wang J, Wang L, Wu Y, Wang J, Lv T, Liu H
PloS one10(6)e0129563(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that levels of CHRM2 are lower in Brodmann's area 24 from subjects with mood disorders, and it is possible that signalling by that receptor is also less in this cortical region; increasing levels of CHRM2 may be involved in the mechanisms of action of mood stabilisers and tricyclic antidepressants
Title: Changes in Muscarinic M2 Receptor Levels in the Cortex of Subjects with Bipolar Disorder and Major Depressive Disorder and in Rats after Treatment with Mood Stabilisers and Antidepressants.

The expression of alpha-SMA mRNA in detrusor muscle cells was 200 times higher than that in urothelial cells in controls.
Title: Laser-capture microdissection for analysis of cell type-specific gene expression of muscarinic receptor subtypes in the rat bladder with cyclophosphamide-induced cystitis.

Neonatal hypoglycemia decreased total muscarinic receptors (p < 0.001) with reduced muscarinic M1, M2, and M3 receptor genes (p < 0.001) in one month old rats.
Title: Cortical cholinergic dysregulation as a long-term consequence of neonatal hypoglycemia.

Long-term stimulation with autoantibodies against M2 muscarinic receptor leaded to the ventricular dilatation and gradual deterioration of cardiac dysfunction.
Title: Mitochondrial Ultrastructural Alterations and Declined M2 Receptor Density Were Involved in Cardiac Dysfunction in Rats after Long Term Treatment with Autoantibodies against M2 Muscarinic Receptor.

This study demonistrated that brain cholinergic receptors and acetylcholinesterase activity in rats following early life arsenic exposure.
Title: Reversibility of changes in brain cholinergic receptors and acetylcholinesterase activity in rats following early life arsenic exposure.

The following Chrm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12051	NM_031016.1 Latest version!	Rattus norvegicus cholinergic receptor, muscarinic 2 (Chrm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$272.30 $389.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrm2 ( NM_031016.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112278.1 Rattus norvegicus cholinergic receptor, muscarinic 2 (Chrm2), mRNA.

Price & Availability↑

CloneID	ORa12051
Clone ID Related Accession (Same CDS sequence)	NM_031016.1
Accession Version	NM_031016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	muscarinic acetylcholine receptor M2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03025.1. On Sep 13, 2012 this sequence version replaced XM_003749715.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J03025.1 [ECO:0000332] ##Evidence-Data-END##

ATGAATAACT CAACAAACTC CTCGAACAAT GGCTTGGCTA TTACCAGTCC TTACAAGACA 
TTTGAAGTGG TATTTATTGT CCTTGTGGCT GGATCCCTCA GTCTGGTGAC CATCATTGGG 
AACATTCTGG TCATGGTTTC CATTAAAGTC AGCCGCCACC TTCAGACTGT CAACAATTAC 
TTCTTGTTCA GCCTGGCCTG TGCTGACCTC ATCATAGGTG TTTTCTCCAT GAACTTGTAT 
ACCCTCTACA CTGTGATTGG CTACTGGCCT TTGGGACCTG TAGTATGTGA CCTATGGCTA 
GCATTGGACT ATGTTGTCAG CAATGCCTCC GTTATGAATC TCCTCATCAT CAGCTTTGAT 
AGATACTTCT GTGTCACGAA ACCTCTGACC TACCCAGTTA AGCGGACCAC AAAAATGGCA 
GGCATGATGA TTGCAGCTGC GTGGGTCCTT TCCTTCATCC TCTGGGCCCC AGCCATTCTC 
TTCTGGCAGT TCATCGTAGG GGTGAGGACT GTGGAGGATG GGGAATGCTA TATTCAGTTC 
TTTTCCAATG CGGCCGTCAC CTTCGGCACT GCCATTGCAG CGTTCTATCT GCCTGTCATC 
ATCATGACTG TGCTCTATTG GCATATATCC CGGGCAAGCA AGAGTAGAAT AAAGAAGGAA 
AAGAAGGAAC CTGTGGCCAA CCAAGACCCA GTATCTCCAA GTCTGGTGCA AGGAAGAATT 
GTAAAGCCAA ACAATAACAA TATGCCTGGT GGTGATGGCG GCCTGGAACA CAACAAGATC 
CAGAATGGCA AGGCTCCACG GGACGGCGTG ACTGAAACCT GTGTTCAAGG GGAGGAGAAA 
GAGAGCTCCA ATGATTCGAC GTCATCAGCT GCTGTGGCCT CCAATATGAG AGATGATGAG 
ATAACCCAGG ATGAAAACAC AGTTTCCACT TCGCTGGACC ACTCCAGAGA TGACAACTCT 
AAGCAAACAT GCATCAAAAT TGTCACCAAG GCCCAAAAAG GTGATGTGTA CACCCCAACG 
AGTACCACTG TAGAACTAGT TGGGTCGTCG GGTCAGAGTG GGGATGAAAA GCAGAACGTT 
GTAGCCCGCA AAATCGTGAA GATGCCCAAG CAGCCTGCCA AAAAGAAGCC TCCACCATCC 
CGGGAAAAGA AAGTGACCAG GACAATCTTG GCTATCCTGT TGGCTTTCAT CATAACGTGG 
GCGCCATACA ATGTCATGGT GCTCATCAAT ACTTTCTGTG CACCCTGCAT CCCCAATACA 
GTATGGACAA TTGGCTACTG GCTCTGTTAC ATCAATAGCA CCATCAATCC GGCCTGCTAT 
GCGCTTTGTA ATGCCACCTT CAAAAAGACT TTTAAGCACC TCCTCATGTG TCATTACAAG 
AACATAGGCG CTACACGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112278.1
CDS	452..1852
Translation	MNNSTNSSNNGLAITSPYKTFEVVFIVLVAGSLSLVTIIGNILVMVSIKVSRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGVRTVEDGECYIQFFSNAAVTFGTAIAAFYLPVIIMTVLYWHISRASKSRIKKEKKEPVANQDPVSPSLVQGRIVKPNNNNMPGGDGGLEHNKIQNGKAPRDGVTETCVQGEEKESSNDSTSSAAVASNMRDDEITQDENTVSTSLDHSRDDNSKQTCIKIVTKAQKGDVYTPTSTTVELVGSSGQSGDEKQNVVARKIVKMPKQPAKKKPPPSREKKVTRTILAILLAFIITWAPYNVMVLINTFCAPCIPNTVWTIGYWLCYINSTINPACYALCNATFKKTFKHLLMCHYKNIGATR

Target ORF information:

RefSeq Version	NM_031016.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor, muscarinic 2 (Chrm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031016.1

ORF Insert Sequence:

ATGAATAACT CAACAAACTC CTCGAACAAT GGCTTGGCTA TTACCAGTCC TTACAAGACA 
TTTGAAGTGG TATTTATTGT CCTTGTGGCT GGATCCCTCA GTCTGGTGAC CATCATTGGG 
AACATTCTGG TCATGGTTTC CATTAAAGTC AGCCGCCACC TTCAGACTGT CAACAATTAC 
TTCTTGTTCA GCCTGGCCTG TGCTGACCTC ATCATAGGTG TTTTCTCCAT GAACTTGTAT 
ACCCTCTACA CTGTGATTGG CTACTGGCCT TTGGGACCTG TAGTATGTGA CCTATGGCTA 
GCATTGGACT ATGTTGTCAG CAATGCCTCC GTTATGAATC TCCTCATCAT CAGCTTTGAT 
AGATACTTCT GTGTCACGAA ACCTCTGACC TACCCAGTTA AGCGGACCAC AAAAATGGCA 
GGCATGATGA TTGCAGCTGC GTGGGTCCTT TCCTTCATCC TCTGGGCCCC AGCCATTCTC 
TTCTGGCAGT TCATCGTAGG GGTGAGGACT GTGGAGGATG GGGAATGCTA TATTCAGTTC 
TTTTCCAATG CGGCCGTCAC CTTCGGCACT GCCATTGCAG CGTTCTATCT GCCTGTCATC 
ATCATGACTG TGCTCTATTG GCATATATCC CGGGCAAGCA AGAGTAGAAT AAAGAAGGAA 
AAGAAGGAAC CTGTGGCCAA CCAAGACCCA GTATCTCCAA GTCTGGTGCA AGGAAGAATT 
GTAAAGCCAA ACAATAACAA TATGCCTGGT GGTGATGGCG GCCTGGAACA CAACAAGATC 
CAGAATGGCA AGGCTCCACG GGACGGCGTG ACTGAAACCT GTGTTCAAGG GGAGGAGAAA 
GAGAGCTCCA ATGATTCGAC GTCATCAGCT GCTGTGGCCT CCAATATGAG AGATGATGAG 
ATAACCCAGG ATGAAAACAC AGTTTCCACT TCGCTGGACC ACTCCAGAGA TGACAACTCT 
AAGCAAACAT GCATCAAAAT TGTCACCAAG GCCCAAAAAG GTGATGTGTA CACCCCAACG 
AGTACCACTG TAGAACTAGT TGGGTCGTCG GGTCAGAGTG GGGATGAAAA GCAGAACGTT 
GTAGCCCGCA AAATCGTGAA GATGCCCAAG CAGCCTGCCA AAAAGAAGCC TCCACCATCC 
CGGGAAAAGA AAGTGACCAG GACAATCTTG GCTATCCTGT TGGCTTTCAT CATAACGTGG 
GCGCCATACA ATGTCATGGT GCTCATCAAT ACTTTCTGTG CACCCTGCAT CCCCAATACA 
GTATGGACAA TTGGCTACTG GCTCTGTTAC ATCAATAGCA CCATCAATCC GGCCTGCTAT 
GCGCTTTGTA ATGCCACCTT CAAAAAGACT TTTAAGCACC TCCTCATGTG TCATTACAAG 
AACATAGGCG CTACACGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

M2 muscarinic receptor activation inhibits cell proliferation and migration of rat adipose-mesenchymal stem cells.
Journal of cellular physiology(2017 Dec)
Piovesana R,Melfi S,Fiore M,Magnaghi V,Tata AM

Changes in Muscarinic M2 Receptor Levels in the Cortex of Subjects with Bipolar Disorder and Major Depressive Disorder and in Rats after Treatment with Mood Stabilisers and Antidepressants.
The international journal of neuropsychopharmacology19(4)(2016 Apr)
Gibbons AS,Jeon WJ,Scarr E,Dean B

Laser-capture microdissection for analysis of cell type-specific gene expression of muscarinic receptor subtypes in the rat bladder with cyclophosphamide-induced cystitis.
International urology and nephrology47(4)637-42(2015 Apr)
Sugino Y,O'Malley KJ,Wang Z,Tyagi P,Birder LA,Ogawa O,Yoshimura N

Cortical cholinergic dysregulation as a long-term consequence of neonatal hypoglycemia.
Biochemistry and cell biology = Biochimie et biologie cellulaire93(1)47-53(2015 Feb)
Anju TR,Paulose CS

Mitochondrial Ultrastructural Alterations and Declined M2 Receptor Density Were Involved in Cardiac Dysfunction in Rats after Long Term Treatment with Autoantibodies against M2 Muscarinic Receptor.
PloS one10(6)e0129563(2015)
Zhang S,He Z,Wang J,Wang L,Wu Y,Wang J,Lv T,Liu H

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Chrm2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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