Grb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grb2
Entrez Gene ID	81504
Full Name	growth factor receptor bound protein 2
Synonyms	Ash-psi
General protein information	Preferred Names growth factor receptor bound protein 2 Names growth factor receptor-bound protein 2 SH2/SH3 adapter GRB2 adapter protein GRB2 protein Ash
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	acts as a link between tyrosine kinase receptors and Ras signaling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_030846.2	NP_110473.2	growth factor receptor-bound protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GRB2	NP_001233546.1
Canis lupus familiaris (dog)	GRB2	XP_540431.2
Rattus norvegicus (Norway rat)	Grb2	NP_110473.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011768	XP_320742.3
Caenorhabditis elegans (roundworm)	sem-5	NP_509342.1
Drosophila melanogaster (fruit fly)	drk	NP_725306.1
Danio rerio (zebrafish)	grb2b	NP_998200.1
Mus musculus (house mouse)	Grb2	NP_032189.1
Gallus gallus (chicken)	GRB2	NP_989742.1
Xenopus tropicalis (tropical clawed frog)	grb2	NP_001008130.1
Homo sapiens (human)	GRB2	NP_002077.1
Bos taurus (cattle)	GRB2	NP_001029802.1

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of MUC1 and Genomic Alterations in Its Network Associate with Prostate Cancer Progression.
Lin X, Gu Y, Kapoor A, Wei F, Aziz T, Ojo D, Jiang Y, Bonert M, Shayegan B, Yang H, Al-Nedawi K, Major P, Tang D
Neoplasia (New York, N.Y.)19(11)857-867(2017 Nov)

Role of growth factor receptor-bound 2 in CCl
Ge S, Xiong Y, Wu X, Xie J, Liu F, He J, Xiang T, Cheng N, Lai L, Zhong Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie92942-951(2017 Aug)

Altered expression of IRS2 and GRB2 in demyelination of peripheral neurons: Implications in diabetic neuropathy.
Manu MS, Rachana KS, Advirao GM
Neuropeptides6271-79(2017 Apr)

miR-96 inhibits cardiac hypertrophy by targeting growth factor receptor-bound 2.
Xia Y, Sheng J, Liang GY, Liu DX, Tang Q, Cheng AP
Genetics and molecular research : GMR14(4)18958-64(2015 Dec)

Grb2 monomer-dimer equilibrium determines normal versus oncogenic function.
Ahmed Z, Timsah Z, Suen KM, Cook NP, Lee GR 4th, Lin CC, Gagea M, Marti AA, Ladbury JE
Nature communications67354(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Growth factor receptor-bound 2 is a direct target of miR-96, which is a negative regulator of cardiac hypertrophy.
Title: miR-96 inhibits cardiac hypertrophy by targeting growth factor receptor-bound 2.

Study confirmed that miR-19b provided a novel mechanism for regulating liver stellate cells proliferation through the targeting of GRB2.
Title: MicroRNA-19b reduces hepatic stellate cell proliferation by targeting GRB2 in hepatic fibrosis models in vivo and in vitro as part of the inhibitory effect of estradiol.

Neph1 but not nephrin specifically binds to adaptor protein Grb2 and tyrosine kinase Csk in a phosphorylation-dependent manner.
Title: Neph1, a component of the kidney slit diaphragm, is tyrosine-phosphorylated by the Src family tyrosine kinase and modulates intracellular signaling by binding to Grb2.

These results suggest that DISC1 is required for NT-3-induced axon elongation and ERK activation at the distal part of axons by recruiting Grb2 to axonal tips.
Title: DISC1 regulates neurotrophin-induced axon elongation via interaction with Grb2.

Free fatty acids may inhibit insulin activity in rat islet cells through up-regulating the expression of cPKCalpha or down-regulating the expression of Grb2 and ERK2.
Title: [Effects of free fatty acids on insulin signaling proteins in rat islet cells].

The following Grb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07181	NM_030846.2 Latest version!	Rattus norvegicus growth factor receptor bound protein 2 (Grb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grb2 ( NM_030846.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_110473.2 Rattus norvegicus growth factor receptor bound protein 2 (Grb2), mRNA.

Price & Availability↑

CloneID	ORa07181
Clone ID Related Accession (Same CDS sequence)	NM_030846.2
Accession Version	NM_030846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	growth factor receptor-bound protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA926364.1 and BC091144.1. On or before Dec 15, 2013 this sequence version replaced XM_006247767.1, XM_003750955.1, XM_003750956.1, NM_030846.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01906351, SAMN01906352 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCCA TCGCCAAATA TGACTTCAAA GCTACTGCTG ACGATGAGCT GAGCTTCAAG 
AGGGGGGACA TCCTTAAGGT TTTGAATGAA GAGTGTGATC AGAACTGGTA TAAGGCAGAA 
CTCAATGGGA AAGATGGCTT CATCCCCAAG AATTACATAG AAATGAAACC ACATCCGTGG 
TTTTTTGGCA AAATCCCCAG AGCAAAGGCA GAAGAAATGC TTAGCAAACA GCGGCATGAC 
GGGGCCTTCC TGATCCGAGA GAGCGAGAGC GCTCCTGGGG ATTTCTCCCT GTCAGTCAAG 
TTTGGAAATG ATGTACAGCA CTTCAAGGTG CTTCGTGACG GAGCCGGGAA GTACTTCCTG 
TGGGTGGTGA AGTTTAATTC TTTGAATGAG CTGGTAGATT ACCACAGATC AACATCCGTG 
TCCAGGAACC AGCAGATATT CCTCCGGGAC ATAGAACAGG TGCCACAGCA GCCAACGTAC 
GTCCAGGCAC TCTTTGACTT TGACCCCCAG GAGGATGGTG AGCTGGGCTT TCGCCGAGGA 
GACTTCATTC ATGTCATGGA TAACTCAGAT CCCAATTGGT GGAAAGGGGC CTGCCATGGG 
CAGACCGGCA TGTTTCCCCG CAATTATGTC ACCCCAGTGA ACCGGAACGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_110473.2
CDS	188..841
Translation	MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV

Target ORF information:

RefSeq Version	NM_030846.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor receptor bound protein 2 (Grb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030846.2

ORF Insert Sequence:

ATGGAAGCCA TCGCCAAATA TGACTTCAAA GCTACTGCTG ACGATGAGCT GAGCTTCAAG 
AGGGGGGACA TCCTTAAGGT TTTGAATGAA GAGTGTGATC AGAACTGGTA TAAGGCAGAA 
CTCAATGGGA AAGATGGCTT CATCCCCAAG AATTACATAG AAATGAAACC ACATCCGTGG 
TTTTTTGGCA AAATCCCCAG AGCAAAGGCA GAAGAAATGC TTAGCAAACA GCGGCATGAC 
GGGGCCTTCC TGATCCGAGA GAGCGAGAGC GCTCCTGGGG ATTTCTCCCT GTCAGTCAAG 
TTTGGAAATG ATGTACAGCA CTTCAAGGTG CTTCGTGACG GAGCCGGGAA GTACTTCCTG 
TGGGTGGTGA AGTTTAATTC TTTGAATGAG CTGGTAGATT ACCACAGATC AACATCCGTG 
TCCAGGAACC AGCAGATATT CCTCCGGGAC ATAGAACAGG TGCCACAGCA GCCAACGTAC 
GTCCAGGCAC TCTTTGACTT TGACCCCCAG GAGGATGGTG AGCTGGGCTT TCGCCGAGGA 
GACTTCATTC ATGTCATGGA TAACTCAGAT CCCAATTGGT GGAAAGGGGC CTGCCATGGG 
CAGACCGGCA TGTTTCCCCG CAATTATGTC ACCCCAGTGA ACCGGAACGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression of MUC1 and Genomic Alterations in Its Network Associate with Prostate Cancer Progression.
Neoplasia (New York, N.Y.)19(11)857-867(2017 Nov)
Lin X,Gu Y,Kapoor A,Wei F,Aziz T,Ojo D,Jiang Y,Bonert M,Shayegan B,Yang H,Al-Nedawi K,Major P,Tang D

Role of growth factor receptor-bound 2 in CCl
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie92942-951(2017 Aug)
Ge S,Xiong Y,Wu X,Xie J,Liu F,He J,Xiang T,Cheng N,Lai L,Zhong Y

Altered expression of IRS2 and GRB2 in demyelination of peripheral neurons: Implications in diabetic neuropathy.
Neuropeptides6271-79(2017 Apr)
Manu MS,Rachana KS,Advirao GM

miR-96 inhibits cardiac hypertrophy by targeting growth factor receptor-bound 2.
Genetics and molecular research : GMR14(4)18958-64(2015 Dec)
Xia Y,Sheng J,Liang GY,Liu DX,Tang Q,Cheng AP

Grb2 monomer-dimer equilibrium determines normal versus oncogenic function.
Nature communications67354(2015 Jun)
Ahmed Z,Timsah Z,Suen KM,Cook NP,Lee GR 4th,Lin CC,Gagea M,Marti AA,Ladbury JE

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Grb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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