Bhlhe40 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bhlhe40
Entrez Gene ID	79431
Full Name	basic helix-loop-helix family, member e40
Synonyms	Bhlhb2,Dec1,SHARP-2,Sharp2,Stra13,Stra14
General protein information	Preferred Names basic helix-loop-helix family, member e40 Names class E basic helix-loop-helix protein 40 basic helix-loop-helix domain containing, class B2 class B basic helix-loop-helix protein 2 enhancer-of-split and hairy-related protein 2 stimulated by retinoic acid 14
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q41
Summary	This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with Arntl or compete for E-box binding sites in the promoter of Per1 and repress Clock/Arntl's transactivation of Per1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. [provided by RefSeq, Feb 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053328.1	NP_445780.1	class E basic helix-loop-helix protein 40

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BHLHE40	XP_516248.3
Homo sapiens (human)	BHLHE40	NP_003661.1
Mus musculus (house mouse)	Bhlhe40	NP_035628.1
Rattus norvegicus (Norway rat)	Bhlhe40	NP_445780.1
Canis lupus familiaris (dog)	BHLHE40	XP_541795.2
Bos taurus (cattle)	BHLHE40	NP_001020100.1
Gallus gallus (chicken)	BHLHE40	NP_001264073.1
	BHLHE40	XP_001095506.2
Danio rerio (zebrafish)	bhlhe40	NP_997844.2

Gene Ontology
Bibliography

Related articles in PubMed

Circadian rhythmicity: A functional connection between differentiated embryonic chondrocyte-1 (DEC1) and small heterodimer partner (SHP).
Marczak MM, Yan B
Archives of biochemistry and biophysics63111-18(2017 Oct)

Nr3C1-Bhlhb2 Axis Dysregulation Is Involved in the Development of Attention Deficit Hyperactivity.
Wu LH, Cheng W, Yu M, He BM, Sun H, Chen Q, Dong YW, Shao XT, Cai QQ, Peng M, Wu XZ
Molecular neurobiology54(2)1196-1212(2017 Mar)

Induction of the SHARP-2 mRNA level by insulin is mediated by multiple signaling pathways.
Kanai Y, Asano K, Komatsu Y, Takagi K, Ono M, Tanaka T, Tomita K, Haneishi A, Tsukada A, Yamada K
Bioscience, biotechnology, and biochemistry81(2)256-261(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

insulin induces the expression of the rat SHARP-2 gene at the transcription level via both a PI 3-K/aPKClambda- and a PI 3-K/mTOR- pathways
Title: Induction of the SHARP-2 mRNA level by insulin is mediated by multiple signaling pathways.

5-Aminoimidazole-4-carboxyamide-1-beta-D-ribofuranoside-induction of the SHARP-2 gene is mediated at transcription level by a phosphoinositide 3-kinase/atypical protein kinase C lambda pathway.
Title: 5-Aminoimidazole-4-carboxyamide-1-?-D-ribofranoside stimulates the rat enhancer of split- and hairy-related protein-2 gene via atypical protein kinase C lambda.

Results describe significant dissociation of expression patterns with arrhythmic expression of Dec1 and rhythmic expression of Dec2 in rat kidney under a normal light-dark (LD) cycle.
Title: Significant dissociation of expression patterns of the basic helix-loop-helix transcription factors Dec1 and Dec2 in rat kidney.

A 30 min feeding stimulus was sufficient to significantly induce the expression of Per2 and Dec1 within 1 h and alter the transcript levels and circadian phases of other selected clock genes in the liver clock at longer time intervals.
Title: Feeding-induced rapid resetting of the hepatic circadian clock is associated with acute induction of Per2 and Dec1 transcription in rats.

These results indicate that ALK-SMAD3-Dec1 signalling provides an input pathway in the mammalian molecular clock.
Title: Activation of TGF-beta/activin signalling resets the circadian clock through rapid induction of Dec1 transcripts.

The following Bhlhe40 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bhlhe40 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09290	NM_053328.1 Latest version!	Rattus norvegicus basic helix-loop-helix family, member e40 (Bhlhe40), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bhlhe40 ( NM_053328.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445780.1 Rattus norvegicus basic helix-loop-helix family, member e40 (Bhlhe40), mRNA.

Price & Availability↑

CloneID	ORa09290
Clone ID Related Accession (Same CDS sequence)	NM_053328.1
Accession Version	NM_053328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	class E basic helix-loop-helix protein 40
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF009330.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF009330.1, FQ238383.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGGA TCCCCAGCGC GCAACCACCT CCTACCTGCC TGCCCAAAAC GCCAGGGCTG 
GAGCACGGAG ACCTGTCAGG GATGGATTTT GCCCACATGT ACCAAGTGTA CAAGTCCAGG 
CGGGGAATAA AACGGAGCGA GGACAGCAAG GAAACTTACA AATTGCCGCA CCGGTTGATT 
GAGAAAAAGA GACGTGACCG GATTAACGAG TGCATTGCCC AGCTGAAGGA TCTCCTACCC 
GAACATCTCA AACTTACTAC TTTGGGTCAC TTGGAGAAAG CAGTGGTTCT CGAGCTGACG 
CTGAAGCACG TGAAAGCATT GACAAACCTA ATTGATCAGC AGCAGCAGAA AATCATGGCC 
CTGCAGAGCG GTTTACAAGC TGGTGATCTG TCGGGAAAGA ATATTGAGGC AGGACAAGAA 
ATGTTCTGCT CCGGTTTCCA GACCTGTGCC CGGGAGGTAC TTCAGTACCT GGCCAAGCAT 
GAAAACACTA GGGACCTGAA GTCTTCCCAG CTTGTCACTC ATCTCCACCG TGTGGTCTCT 
GAACTCCTGC AGGGTAGTGC TTCCAGGAAA CCATTGGACT CAGCTCCCAA ACCCGTGGAC 
TTCAAAGAGA AGCCCAGCTT CCTAGCCAAG GGATCAGAAG GCCCTGGGAA AAACTGTGTG 
CCAGTCATCC AGAGGACTTT TGCTCCCTCG GGCGGGGAGC AGAGTGGTAG TGACACGGAC 
ACAGACAGTG GCTACGGAGG CGAATTGGAG AAGGGTGACT TGCGCAGTGA GCAACCCTAC 
TTCAAGAGCG ATCACGGACG CAGGTTCACC GTGGGAGAAC GCGTCAGCAC AATTAAGCAA 
GAATCTGAAG AGCCCCCCAC CAAAAAGAGC CGAATGCAGC TCTCAGATGA GGAAGGCCAC 
TTCGTGGGCA GTGACCTGAT GGGTTCCCCA TTTCTTGGGC CTCACCCACA TCAGCCTCCC 
TTTTGCCTGC CCTTCTATCT CATCCCACCA TCGGCCACTG CCTATCTGCC TATGCTGGAG 
AAATGCTGGT ATCCGACCTC TGTGCCACTG TTATACCCAG GCCTCAACAC CTCAGCAGCA 
GCCCTCTCCA GCTTCATGAA CCCAGACAAG ATCCCAACTC CCTTGCTCCT GCCCCAGAGA 
CTCCCTTCTC CCTTGGCACA TTCGTCCCTT GACTCTTCAG CCTTGCTCCA GGCCCTGAAG 
CAGATCCCTC CTTTAAACTT AGAAACCAAA GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445780.1
CDS	320..1555
Translation	MERIPSAQPPPTCLPKTPGLEHGDLSGMDFAHMYQVYKSRRGIKRSEDSKETYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHVKALTNLIDQQQQKIMALQSGLQAGDLSGKNIEAGQEMFCSGFQTCAREVLQYLAKHENTRDLKSSQLVTHLHRVVSELLQGSASRKPLDSAPKPVDFKEKPSFLAKGSEGPGKNCVPVIQRTFAPSGGEQSGSDTDTDSGYGGELEKGDLRSEQPYFKSDHGRRFTVGERVSTIKQESEEPPTKKSRMQLSDEEGHFVGSDLMGSPFLGPHPHQPPFCLPFYLIPPSATAYLPMLEKCWYPTSVPLLYPGLNTSAAALSSFMNPDKIPTPLLLPQRLPSPLAHSSLDSSALLQALKQIPPLNLETKD

Target ORF information:

RefSeq Version	NM_053328.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus basic helix-loop-helix family, member e40 (Bhlhe40), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053328.1

ORF Insert Sequence:

ATGGAGCGGA TCCCCAGCGC GCAACCACCT CCTACCTGCC TGCCCAAAAC GCCAGGGCTG 
GAGCACGGAG ACCTGTCAGG GATGGATTTT GCCCACATGT ACCAAGTGTA CAAGTCCAGG 
CGGGGAATAA AACGGAGCGA GGACAGCAAG GAAACTTACA AATTGCCGCA CCGGTTGATT 
GAGAAAAAGA GACGTGACCG GATTAACGAG TGCATTGCCC AGCTGAAGGA TCTCCTACCC 
GAACATCTCA AACTTACTAC TTTGGGTCAC TTGGAGAAAG CAGTGGTTCT CGAGCTGACG 
CTGAAGCACG TGAAAGCATT GACAAACCTA ATTGATCAGC AGCAGCAGAA AATCATGGCC 
CTGCAGAGCG GTTTACAAGC TGGTGATCTG TCGGGAAAGA ATATTGAGGC AGGACAAGAA 
ATGTTCTGCT CCGGTTTCCA GACCTGTGCC CGGGAGGTAC TTCAGTACCT GGCCAAGCAT 
GAAAACACTA GGGACCTGAA GTCTTCCCAG CTTGTCACTC ATCTCCACCG TGTGGTCTCT 
GAACTCCTGC AGGGTAGTGC TTCCAGGAAA CCATTGGACT CAGCTCCCAA ACCCGTGGAC 
TTCAAAGAGA AGCCCAGCTT CCTAGCCAAG GGATCAGAAG GCCCTGGGAA AAACTGTGTG 
CCAGTCATCC AGAGGACTTT TGCTCCCTCG GGCGGGGAGC AGAGTGGTAG TGACACGGAC 
ACAGACAGTG GCTACGGAGG CGAATTGGAG AAGGGTGACT TGCGCAGTGA GCAACCCTAC 
TTCAAGAGCG ATCACGGACG CAGGTTCACC GTGGGAGAAC GCGTCAGCAC AATTAAGCAA 
GAATCTGAAG AGCCCCCCAC CAAAAAGAGC CGAATGCAGC TCTCAGATGA GGAAGGCCAC 
TTCGTGGGCA GTGACCTGAT GGGTTCCCCA TTTCTTGGGC CTCACCCACA TCAGCCTCCC 
TTTTGCCTGC CCTTCTATCT CATCCCACCA TCGGCCACTG CCTATCTGCC TATGCTGGAG 
AAATGCTGGT ATCCGACCTC TGTGCCACTG TTATACCCAG GCCTCAACAC CTCAGCAGCA 
GCCCTCTCCA GCTTCATGAA CCCAGACAAG ATCCCAACTC CCTTGCTCCT GCCCCAGAGA 
CTCCCTTCTC CCTTGGCACA TTCGTCCCTT GACTCTTCAG CCTTGCTCCA GGCCCTGAAG 
CAGATCCCTC CTTTAAACTT AGAAACCAAA GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Circadian rhythmicity: A functional connection between differentiated embryonic chondrocyte-1 (DEC1) and small heterodimer partner (SHP).
Archives of biochemistry and biophysics63111-18(2017 Oct)
Marczak MM,Yan B

Nr3C1-Bhlhb2 Axis Dysregulation Is Involved in the Development of Attention Deficit Hyperactivity.
Molecular neurobiology54(2)1196-1212(2017 Mar)
Wu LH,Cheng W,Yu M,He BM,Sun H,Chen Q,Dong YW,Shao XT,Cai QQ,Peng M,Wu XZ

Induction of the SHARP-2 mRNA level by insulin is mediated by multiple signaling pathways.
Bioscience, biotechnology, and biochemistry81(2)256-261(2017 Feb)
Kanai Y,Asano K,Komatsu Y,Takagi K,Ono M,Tanaka T,Tomita K,Haneishi A,Tsukada A,Yamada K

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Bhlhe40 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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