Akap4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akap4
Entrez Gene ID	79254
Full Name	A-kinase anchoring protein 4
General protein information	Preferred Names A-kinase anchoring protein 4 Names A-kinase anchor protein 4 75 kDa fibrous sheath protein A kinase (PRKA) anchor protein 4 AKAP-4 PRKA4 major sperm fibrous sheath protein protein kinase A-anchoring protein 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq12

mRNA and Protein(s)

mRNA	Protein	Name
NM_024402.1	NP_077378.1	A-kinase anchor protein 4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AKAP4	XP_851545.1
Mus musculus (house mouse)	Akap4	NP_033781.2
Rattus norvegicus (Norway rat)	Akap4	NP_077378.1
	LOC694888	XP_002808575.1
Homo sapiens (human)	AKAP4	NP_003877.2
Pan troglodytes (chimpanzee)	AKAP4	XP_521367.3
Bos taurus (cattle)	AKAP4	NP_776660.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of SPEF2 function in mice results in spermatogenesis defects and primary ciliary dyskinesia.
Sironen A, Kotaja N, Mulhern H, Wyatt TA, Sisson JH, Pavlik JA, Miiluniemi M, Fleming MD, Lee L
Biology of reproduction85(4)690-701(2011 Oct)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

A-kinase anchoring proteins: from protein complexes to physiology and disease.
Carnegie GK, Means CK, Scott JD
IUBMB life61(4)394-406(2009 Apr)

Protein kinase A, Ca2+/calmodulin-dependent kinase II, and calcineurin regulate the intracellular trafficking of myopodin between the Z-disc and the nucleus of cardiac myocytes.
Faul C, Dhume A, Schecter AD, Mundel P
Molecular and cellular biology27(23)8215-27(2007 Dec)

Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa.
Krisfalusi M, Miki K, Magyar PL, O'Brien DA
Biology of reproduction75(2)270-8(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Akap4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akap4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42504	NM_024402.1 Latest version!	Rattus norvegicus A-kinase anchoring protein 4 (Akap4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akap4 ( NM_024402.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077378.1 Rattus norvegicus A-kinase anchoring protein 4 (Akap4), mRNA.

Price & Availability↑

CloneID	ORa42504
Clone ID Related Accession (Same CDS sequence)	NM_024402.1
Accession Version	NM_024402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF008114.1. On Dec 17, 2003 this sequence version replaced XM_346866.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF008114.1 [ECO:0000332] ##Evidence-Data-END##

ATGATTGCTT ACTGTGGCAC TACAAAGATG TCTGATGACA TTGACTGGTT ACACAGCCGC 
AGAGGCGTGT GCAAGGTAGA TCTTTACAGC CCAGAAGGAC AACAAGATCA GGACCGAAAA 
GTGATATGCT TTGTGGATGT GTCCACCTTG AATGTGGAAG ATGATTCCAA GGGTGCTGCT 
GGTCCCAGGT CAGACGGCGA ATTAAACCTG GAGAATCTGG AAGAAAAAGA GATTATCGTG 
ATCAAGGACA CAGAAAAACA AGACCAGCCT AAGACGGAGG GATCTGTATG CCTTTTCAAA 
CAAGCTCCCT CTGACCCCAT AAGTGTCCTC AACTGGCTCC TCAATGATCT CCAGAAGTAC 
GCCTTGGGTT TCCAACATGC ATTAAGTCCC TCAGCCTCTA GCTGTAAACA TAAAGTAGGA 
GACCTAGAGG GTGATTATCA CAAAATTCCC TCAGAGAACT GCTACAGTGT GTATGCTGAT 
CAAGTAAACT TAGATTATTT GAACAAAGGA CCTCAAAACC TTCGCCTAGA AATGGCGGCA 
TCCAAAAATA CCAACAATAA CCAAAGTCCC TCAAATCCTG CAACTAAATC TCCTAGCAAT 
CAGAGGTCAG TTGCCACTCC TGATGGAGAA TGTTCGATGG ACGACCTCTC CTACTATGTC 
AACCGATTGT CATCTCTAGT AATCCAAATG GCCCGTAAGG AAATCAAGGA CAAATTGGAA 
GGTGGAAACA AATGCCTCCA TCATTCCATG TATACATCAG GAGAAAAGGG GAAAACCAGC 
CCCCGGAGTG CTGTCAGCAA AATTGCTTCT GAGATGGCCC ATGAAGCTGT TGAACTGACC 
TCATCAGAAA TGCGTGGCAA TGGAGAAGAA GGCAGGGATG GCCGGAAAAC CTTTTTGTAT 
AGTGAGTTAT CTAATAAGAA CAAGTGTGGA GAGAAGCAGC AGATGTGCCC AAAAGACAGC 
AAAGAATTTG CAGATTCCAT CAGCAAGGGG CTTATGGTTT ATGCAAATCA GGTAGCATCT 
GACATGATGG TCTCTGTTAT GAAAACCTTG AAAGTGCACA GCTGTGGGAA GCCAATTCCA 
GCTTGTGTGG TCCTGAAGAG GGTACTATTA AAGCACACCA AGGAAATTGT ATCTGATCTG 
ATTGACTCAT GCATGAAGAA CTTGCATAAT ATAACAGGAG TCCTGATGAC AGACTCAGAC 
TTCGTTTCTG CTGTCAAGAG GAATCTTTTC AATCATGGTA AACAAAATGC AGCAGACATC 
ATGGAGGCCA TGCTAAAGCG TCTGGTCAGT GCTCTTCTTG GTGAGAAGAA GGAGACTAAA 
TCTCAAAGTT TGGCATATGC AACCTTAAAA GCTGGAACTC ATGACCCGAA ATGCAAGAAT 
CAGAGCCTTG AGTTCTCAGC TATGAAAGCT GAAATGAAAG GAAAGGACAA AGGCAAAACT 
AAAGGAGATC CATGCTGTAA ATCACTGACA AGTGCTGAGA GAGTCAGCGA ACATATCCTC 
AAAGAGAGCC TTACTATGTG GAACAACCAG AAGCAAGGAA CCCAAGGCAG GGTGCCTAAC 
AAAGTATGCC CTAGTAAAGA TGAAAAGAGA GAGAAGATCA GTCCTTCCAC AGACTCACTG 
GCCAAGGATC TAATTGTCTC TGCCCTTATG CTCATTCAAT ACCATCTGAC CCAACAAGCG 
AAGGGCAAAG ATCCATGTGA AGAGGAGTGC CCTGGTTCCT CCATGGGCTA TATGTCGCAA 
AGTGCACAAT ATGAAAAGAG TGGAGGTGGC CAAAGTTCTA AATCACTTTC AATGAAGCAT 
TTTGAATCTC GTGGAGCTCC TGGACCATCT ACATGTGCGA AGGAAAATCA ACTTGAGTCC 
CAGAAGATGG ATATGTCAAA CATGGTTCTG TCCCTGATTC AGAAACTTCT GAGTGAGAGC 
CCTTTCAGCT GTGATGAACT ATCTGAAAGT GAGAATAAGC GTTGTTGTGA TTCTCGATCA 
AAGCAAGCAG CTCCCGTGGC CAAGAGACCT GAAGATCAAA GCCAAGACAG CACAGAAATG 
GACTTCATCA GCGGGATGAA GCAAATGAAC CGGCAGTTTA TAGACCAACT AGTAGAATCG 
GTGATGAAAC TGTGCCTGAT CATGGCTAAG TACAGCAACA ATGGAGCAGC ACTAGCTGAG 
CTAGAAGAGC AAGCAGCCCT AGCAAGCAAT GGCCCCAGAT GTGGCCGTGA GGCTGTGATG 
TCACAAAGTT ATCTGGAAAC TCCTGGCCCT GAAGTTATCG TCAACAATCA GTGCTCTACA 
AGCAACTTGC AGAAGCAGCT GCAGGCTGTC CTGCAATGGA TTGCAGCGTC TCAGTTTAAT 
GTGCCCATGC TCTACTTCAT GGGAGATGAT GATGGACAAC TAGAGAAGCT CCCTGAAGTT 
TCAGCTAAGG CAGCAGAGAA AGGTTACAGT GTAGGAGATC TTCTTCAGGA GGTCATGAAG 
TTTGCCAAAG AACGACAACT GGATGAAGCC GTGGGCAACA TGGCTAGAAA GCAACTGCTG 
GACTGGCTTC TCGCTAACCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077378.1
CDS	55..2598
Translation	MIAYCGTTKMSDDIDWLHSRRGVCKVDLYSPEGQQDQDRKVICFVDVSTLNVEDDSKGAAGPRSDGELNLENLEEKEIIVIKDTEKQDQPKTEGSVCLFKQAPSDPISVLNWLLNDLQKYALGFQHALSPSASSCKHKVGDLEGDYHKIPSENCYSVYADQVNLDYLNKGPQNLRLEMAASKNTNNNQSPSNPATKSPSNQRSVATPDGECSMDDLSYYVNRLSSLVIQMARKEIKDKLEGGNKCLHHSMYTSGEKGKTSPRSAVSKIASEMAHEAVELTSSEMRGNGEEGRDGRKTFLYSELSNKNKCGEKQQMCPKDSKEFADSISKGLMVYANQVASDMMVSVMKTLKVHSCGKPIPACVVLKRVLLKHTKEIVSDLIDSCMKNLHNITGVLMTDSDFVSAVKRNLFNHGKQNAADIMEAMLKRLVSALLGEKKETKSQSLAYATLKAGTHDPKCKNQSLEFSAMKAEMKGKDKGKTKGDPCCKSLTSAERVSEHILKESLTMWNNQKQGTQGRVPNKVCPSKDEKREKISPSTDSLAKDLIVSALMLIQYHLTQQAKGKDPCEEECPGSSMGYMSQSAQYEKSGGGQSSKSLSMKHFESRGAPGPSTCAKENQLESQKMDMSNMVLSLIQKLLSESPFSCDELSESENKRCCDSRSKQAAPVAKRPEDQSQDSTEMDFISGMKQMNRQFIDQLVESVMKLCLIMAKYSNNGAALAELEEQAALASNGPRCGREAVMSQSYLETPGPEVIVNNQCSTSNLQKQLQAVLQWIAASQFNVPMLYFMGDDDGQLEKLPEVSAKAAEKGYSVGDLLQEVMKFAKERQLDEAVGNMARKQLLDWLLANL

Target ORF information:

RefSeq Version	NM_024402.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus A-kinase anchoring protein 4 (Akap4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024402.1

ORF Insert Sequence:

ATGATTGCTT ACTGTGGCAC TACAAAGATG TCTGATGACA TTGACTGGTT ACACAGCCGC 
AGAGGCGTGT GCAAGGTAGA TCTTTACAGC CCAGAAGGAC AACAAGATCA GGACCGAAAA 
GTGATATGCT TTGTGGATGT GTCCACCTTG AATGTGGAAG ATGATTCCAA GGGTGCTGCT 
GGTCCCAGGT CAGACGGCGA ATTAAACCTG GAGAATCTGG AAGAAAAAGA GATTATCGTG 
ATCAAGGACA CAGAAAAACA AGACCAGCCT AAGACGGAGG GATCTGTATG CCTTTTCAAA 
CAAGCTCCCT CTGACCCCAT AAGTGTCCTC AACTGGCTCC TCAATGATCT CCAGAAGTAC 
GCCTTGGGTT TCCAACATGC ATTAAGTCCC TCAGCCTCTA GCTGTAAACA TAAAGTAGGA 
GACCTAGAGG GTGATTATCA CAAAATTCCC TCAGAGAACT GCTACAGTGT GTATGCTGAT 
CAAGTAAACT TAGATTATTT GAACAAAGGA CCTCAAAACC TTCGCCTAGA AATGGCGGCA 
TCCAAAAATA CCAACAATAA CCAAAGTCCC TCAAATCCTG CAACTAAATC TCCTAGCAAT 
CAGAGGTCAG TTGCCACTCC TGATGGAGAA TGTTCGATGG ACGACCTCTC CTACTATGTC 
AACCGATTGT CATCTCTAGT AATCCAAATG GCCCGTAAGG AAATCAAGGA CAAATTGGAA 
GGTGGAAACA AATGCCTCCA TCATTCCATG TATACATCAG GAGAAAAGGG GAAAACCAGC 
CCCCGGAGTG CTGTCAGCAA AATTGCTTCT GAGATGGCCC ATGAAGCTGT TGAACTGACC 
TCATCAGAAA TGCGTGGCAA TGGAGAAGAA GGCAGGGATG GCCGGAAAAC CTTTTTGTAT 
AGTGAGTTAT CTAATAAGAA CAAGTGTGGA GAGAAGCAGC AGATGTGCCC AAAAGACAGC 
AAAGAATTTG CAGATTCCAT CAGCAAGGGG CTTATGGTTT ATGCAAATCA GGTAGCATCT 
GACATGATGG TCTCTGTTAT GAAAACCTTG AAAGTGCACA GCTGTGGGAA GCCAATTCCA 
GCTTGTGTGG TCCTGAAGAG GGTACTATTA AAGCACACCA AGGAAATTGT ATCTGATCTG 
ATTGACTCAT GCATGAAGAA CTTGCATAAT ATAACAGGAG TCCTGATGAC AGACTCAGAC 
TTCGTTTCTG CTGTCAAGAG GAATCTTTTC AATCATGGTA AACAAAATGC AGCAGACATC 
ATGGAGGCCA TGCTAAAGCG TCTGGTCAGT GCTCTTCTTG GTGAGAAGAA GGAGACTAAA 
TCTCAAAGTT TGGCATATGC AACCTTAAAA GCTGGAACTC ATGACCCGAA ATGCAAGAAT 
CAGAGCCTTG AGTTCTCAGC TATGAAAGCT GAAATGAAAG GAAAGGACAA AGGCAAAACT 
AAAGGAGATC CATGCTGTAA ATCACTGACA AGTGCTGAGA GAGTCAGCGA ACATATCCTC 
AAAGAGAGCC TTACTATGTG GAACAACCAG AAGCAAGGAA CCCAAGGCAG GGTGCCTAAC 
AAAGTATGCC CTAGTAAAGA TGAAAAGAGA GAGAAGATCA GTCCTTCCAC AGACTCACTG 
GCCAAGGATC TAATTGTCTC TGCCCTTATG CTCATTCAAT ACCATCTGAC CCAACAAGCG 
AAGGGCAAAG ATCCATGTGA AGAGGAGTGC CCTGGTTCCT CCATGGGCTA TATGTCGCAA 
AGTGCACAAT ATGAAAAGAG TGGAGGTGGC CAAAGTTCTA AATCACTTTC AATGAAGCAT 
TTTGAATCTC GTGGAGCTCC TGGACCATCT ACATGTGCGA AGGAAAATCA ACTTGAGTCC 
CAGAAGATGG ATATGTCAAA CATGGTTCTG TCCCTGATTC AGAAACTTCT GAGTGAGAGC 
CCTTTCAGCT GTGATGAACT ATCTGAAAGT GAGAATAAGC GTTGTTGTGA TTCTCGATCA 
AAGCAAGCAG CTCCCGTGGC CAAGAGACCT GAAGATCAAA GCCAAGACAG CACAGAAATG 
GACTTCATCA GCGGGATGAA GCAAATGAAC CGGCAGTTTA TAGACCAACT AGTAGAATCG 
GTGATGAAAC TGTGCCTGAT CATGGCTAAG TACAGCAACA ATGGAGCAGC ACTAGCTGAG 
CTAGAAGAGC AAGCAGCCCT AGCAAGCAAT GGCCCCAGAT GTGGCCGTGA GGCTGTGATG 
TCACAAAGTT ATCTGGAAAC TCCTGGCCCT GAAGTTATCG TCAACAATCA GTGCTCTACA 
AGCAACTTGC AGAAGCAGCT GCAGGCTGTC CTGCAATGGA TTGCAGCGTC TCAGTTTAAT 
GTGCCCATGC TCTACTTCAT GGGAGATGAT GATGGACAAC TAGAGAAGCT CCCTGAAGTT 
TCAGCTAAGG CAGCAGAGAA AGGTTACAGT GTAGGAGATC TTCTTCAGGA GGTCATGAAG 
TTTGCCAAAG AACGACAACT GGATGAAGCC GTGGGCAACA TGGCTAGAAA GCAACTGCTG 
GACTGGCTTC TCGCTAACCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of SPEF2 function in mice results in spermatogenesis defects and primary ciliary dyskinesia.
Biology of reproduction85(4)690-701(2011 Oct)
Sironen A,Kotaja N,Mulhern H,Wyatt TA,Sisson JH,Pavlik JA,Miiluniemi M,Fleming MD,Lee L

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

A-kinase anchoring proteins: from protein complexes to physiology and disease.
IUBMB life61(4)394-406(2009 Apr)
Carnegie GK,Means CK,Scott JD

Protein kinase A, Ca2+/calmodulin-dependent kinase II, and calcineurin regulate the intracellular trafficking of myopodin between the Z-disc and the nucleus of cardiac myocytes.
Molecular and cellular biology27(23)8215-27(2007 Dec)
Faul C,Dhume A,Schecter AD,Mundel P

Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa.
Biology of reproduction75(2)270-8(2006 Aug)
Krisfalusi M,Miki K,Magyar PL,O'Brien DA

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Akap4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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