Aspa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aspa
Entrez Gene ID	79251
Full Name	aspartoacylase
General protein information	Preferred Names aspartoacylase Names aspartoacylase ACY-2 aminoacylase-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24
Summary	catalyzes the deacetylation of N-acetylaspartate to free acetate; may play a role in central nervous system myelination [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246815.3	XP_006246877.1	aspartoacylase isoform X1
XM_006246817.3	XP_006246879.1	aspartoacylase isoform X3
XM_017597543.1	XP_017453032.1	aspartoacylase isoform X2
NM_024399.1	NP_077375.1	aspartoacylase
XM_006246818.3	XP_006246880.1	aspartoacylase isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ASPA	XP_001158167.1
	ASPA	XP_001089160.1
Mus musculus (house mouse)	Aspa	NP_075602.2
Rattus norvegicus (Norway rat)	Aspa	NP_077375.1
Danio rerio (zebrafish)	aspa	NP_001103573.1
Homo sapiens (human)	ASPA	NP_001121557.1
Canis lupus familiaris (dog)	ASPA	XP_868535.2
Bos taurus (cattle)	ASPA	NP_001039498.1
Gallus gallus (chicken)	ASPA	XP_415853.3
Xenopus tropicalis (tropical clawed frog)	aspa	NP_001039114.1

Gene Ontology
Bibliography

Related articles in PubMed

Involvement of aspartoacylase in tremor expression in rats.
Nishitani A, Tanaka M, Shimizu S, Kunisawa N, Yokoe M, Yoshida Y, Suzuki T, Sakuma T, Yamamoto T, Kuwamura M, Takenaka S, Ohno Y, Kuramoto T
Experimental animals65(3)293-301(2016 Jul)

The molecular mechanisms affecting N-acetylaspartate homeostasis following experimental graded traumatic brain injury.
Di Pietro V, Amorini AM, Tavazzi B, Vagnozzi R, Logan A, Lazzarino G, Signoretti S, Lazzarino G, Belli A
Molecular medicine (Cambridge, Mass.)20147-57(2014 Mar)

Expression and localization of myosin-1d in the developing nervous system.
Benesh AE, Fleming JT, Chiang C, Carter BD, Tyska MJ
Brain research14409-22(2012 Feb)

Extensive aspartoacylase expression in the rat central nervous system.
Moffett JR, Arun P, Ariyannur PS, Garbern JY, Jacobowitz DM, Namboodiri AM
Glia59(10)1414-34(2011 Oct)

Structure of aspartoacylase, the brain enzyme impaired in Canavan disease.
Bitto E, Bingman CA, Wesenberg GE, McCoy JG, Phillips GN Jr
Proceedings of the National Academy of Sciences of the United States of America104(2)456-61(2007 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Interaction of N-acetyl-L-aspartate accumulation due to ASPA deficiency with an unstable neuronal membrane potential caused by HCN1 deficiency was involved in tremor development.
Title: Involvement of aspartoacylase in tremor expression in rats.

The decrease in N-acetylaspartate following severe traumatic brain injury was characterized by profound mitochondrial malfunctioning, dramatic repression of Nat8l gene and concomitant increase in the ASPA gene and protein levels.
Title: The molecular mechanisms affecting N-acetylaspartate homeostasis following experimental graded traumatic brain injury.

This study demonistrated that aspartoacylase expression in the rat white matter central nervous system.
Title: Extensive aspartoacylase expression in the rat central nervous system.

the N-terminal domain of aspartoacylase adopts a protein fold similar to that of zinc-dependent hydrolases related to carboxypeptidases A
Title: Structure of aspartoacylase, the brain enzyme impaired in Canavan disease.

describe the occurrence of ASPA within nuclei of rat brain and kidney, and in cultured rodent oligodendrocytes.
Title: Aspartoacylase is a regulated nuclear-cytoplasmic enzyme.

The following Aspa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aspa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa33389	XM_006246815.3 Latest version!	Rattus norvegicus aspartoacylase (Aspa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa07045	NM_024399.1 Latest version!	Rattus norvegicus aspartoacylase (Aspa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa33391	XM_006246817.3 Latest version!	Rattus norvegicus aspartoacylase (Aspa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa33392	XM_006246818.3 Latest version!	Rattus norvegicus aspartoacylase (Aspa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa57491	XM_017597543.1 Latest version!	Rattus norvegicus aspartoacylase (Aspa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aspa ( XM_006246815.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246877.1 Rattus norvegicus aspartoacylase (Aspa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33389
Clone ID Related Accession (Same CDS sequence)	XM_006246815.3
Accession Version	XM_006246815.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	aspartoacylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246815.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTT GTGTTGCTGA AGAACCTATT AAAAAGATTG CCATCTTCGG AGGGACTCAT 
GGAAATGAAC TGACTGGAGT GTTTCTAGTT ACTCACTGGC TAAAGAATGG CGCTGAAGTT 
CACAGAGCAG GGCTGGAAGT GAAGCCATTC ATTACCAACC CAAGAGCGGT GGAGAAGTGC 
ACCAGATACA TTGACTGTGA CCTGAACCGT GTTTTTGACC TTGAAAATCT TAGCAAAGAG 
ATGTCTGAAG ATTTGCCGTA TGAAGTGAGA AGGGCTCAAG AAATAAATCA CTTATTTGGT 
CCAAAAAATA GTGATGATGC CTACGATGTC GTTTTTGACC TTCATAACAC TACTTCTAAC 
ATGGGTTGCA CTCTTATTCT TGAGGATTCC AGGAATGACT TTTTAATCCA GATGTTTCAC 
TATATTAAGA CCTGCATGGC TCCATTACCC TGCTCTGTTT ACCTCATCGA GCATCCTTCC 
CTCAAATATG CAACCACTCG TTCCATTGCC AAGTATCCTG TTGGTATAGA AGTTGGTCCT 
CAGCCTCACG GTGTTCTGAG AGCTGATATT TTAGACCAAA TGAGAAGAAT GCTCAAGCAC 
GCTCTTGATT TTATACAGCG TTTCAATGAA GGTAAAGAAT TTCCCCCCTG TGCTATTGAT 
GTCTATAAAA TAATGGAGAA AGTTGATTAT CCAAGGAACG AAAGTGGAGA CGTGGCTGCT 
GTTATCCATC CTAATCTGCA GGATCAAGAC TGGAAACCAT TGCACCCTGG AGATCCTGTG 
TTTGTGTCTC TTGATGGAAA AGTTATTCCA CTGGGTGGAG ACTGTACCGT GTACCCAGTG 
TTTGTGAATG AAGCTGCATA TTATGAAAAA AAAGAAGCAT TTGCAAAGAC AACAAAACTA 
ACACTCAACG CAAAAAGCAT CCGCTCCACT TTGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246877.1
CDS	180..1118
Translation	MTSCVAEEPIKKIAIFGGTHGNELTGVFLVTHWLKNGAEVHRAGLEVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEMSEDLPYEVRRAQEINHLFGPKNSDDAYDVVFDLHNTTSNMGCTLILEDSRNDFLIQMFHYIKTCMAPLPCSVYLIEHPSLKYATTRSIAKYPVGIEVGPQPHGVLRADILDQMRRMLKHALDFIQRFNEGKEFPPCAIDVYKIMEKVDYPRNESGDVAAVIHPNLQDQDWKPLHPGDPVFVSLDGKVIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRSTLH

Target ORF information:

RefSeq Version	XM_006246815.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aspartoacylase (Aspa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246815.3

ORF Insert Sequence:

ATGACTTCTT GTGTTGCTGA AGAACCTATT AAAAAGATTG CCATCTTCGG AGGGACTCAT 
GGAAATGAAC TGACTGGAGT GTTTCTAGTT ACTCACTGGC TAAAGAATGG CGCTGAAGTT 
CACAGAGCAG GGCTGGAAGT GAAGCCATTC ATTACCAACC CAAGAGCGGT GGAGAAGTGC 
ACCAGATACA TTGACTGTGA CCTGAACCGT GTTTTTGACC TTGAAAATCT TAGCAAAGAG 
ATGTCTGAAG ATTTGCCGTA TGAAGTGAGA AGGGCTCAAG AAATAAATCA CTTATTTGGT 
CCAAAAAATA GTGATGATGC CTACGATGTC GTTTTTGACC TTCATAACAC TACTTCTAAC 
ATGGGTTGCA CTCTTATTCT TGAGGATTCC AGGAATGACT TTTTAATCCA GATGTTTCAC 
TATATTAAGA CCTGCATGGC TCCATTACCC TGCTCTGTTT ACCTCATCGA GCATCCTTCC 
CTCAAATATG CAACCACTCG TTCCATTGCC AAGTATCCTG TTGGTATAGA AGTTGGTCCT 
CAGCCTCACG GTGTTCTGAG AGCTGATATT TTAGACCAAA TGAGAAGAAT GCTCAAGCAC 
GCTCTTGATT TTATACAGCG TTTCAATGAA GGTAAAGAAT TTCCCCCCTG TGCTATTGAT 
GTCTATAAAA TAATGGAGAA AGTTGATTAT CCAAGGAACG AAAGTGGAGA CGTGGCTGCT 
GTTATCCATC CTAATCTGCA GGATCAAGAC TGGAAACCAT TGCACCCTGG AGATCCTGTG 
TTTGTGTCTC TTGATGGAAA AGTTATTCCA CTGGGTGGAG ACTGTACCGT GTACCCAGTG 
TTTGTGAATG AAGCTGCATA TTATGAAAAA AAAGAAGCAT TTGCAAAGAC AACAAAACTA 
ACACTCAACG CAAAAAGCAT CCGCTCCACT TTGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aspa ( NM_024399.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077375.1 Rattus norvegicus aspartoacylase (Aspa), mRNA.

Price & Availability↑

CloneID	ORa07045
Clone ID Related Accession (Same CDS sequence)	NM_024399.1
Accession Version	NM_024399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	aspartoacylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB023432.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB023432.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGACTTCTT GTGTTGCTGA AGAACCTATT AAAAAGATTG CCATCTTCGG AGGGACTCAT 
GGAAATGAAC TGACTGGAGT GTTTCTAGTT ACTCACTGGC TAAAGAATGG CGCTGAAGTT 
CACAGAGCAG GGCTGGAAGT GAAGCCATTC ATTACCAACC CAAGAGCGGT GGAGAAGTGC 
ACCAGATACA TTGACTGTGA CCTGAACCGT GTTTTTGACC TTGAAAATCT TAGCAAAGAG 
ATGTCTGAAG ATTTGCCGTA TGAAGTTAGA AGGGCTCAAG AAATAAATCA CTTATTTGGT 
CCAAAAGATA GTGATGATGC CTACGATGTC GTTTTTGTCC TTCATAACAC TACTTCTAAC 
ATGGGTTGCA CTCTTATTCT TGAGGATTCC AGGAATGACT TTTTAATCCA GATGTTTCAC 
TATATTAAGA CCTGCATGGC TCCATTACCC TGCTCTGTTT ACCTCATCGA GCATCCTTCC 
CTCAAATATG CAACCACTCG TTCCATTGCC AAGTATCCTG TTGGTATAGA AGTTGGTCCT 
CAGCATCACG GTGTTCTGAG AGCTGATATT TTAGACCAAA TGAGAAGAAT GCTCAAGCAC 
GCTCTTGATT TTATACAGCG TTTCAATGAA GGTAAAGAAT TTCCCCCCTG TGCTATTGAT 
GTCTATAAAA TAATGGAGAA AGTTGATTAT CCAAGGAACG AAAGTGGAGA CGTGGCTGCT 
GTTATCCATC CTAATCTGCA GCTGCAGGAT CAAGACTGGA AACCATTGCA CCCTGGAGAT 
CCTGTGTTTG TGTCTCTTGA TGGAAAAGTT ATTCCACTGG GTGGAGACTG TACCGTGTAC 
CCAGTGTTTG TGAATGAAGC TGCATATTAT GAAAAAAAAG AAGCATTTGC AAAGACAACA 
AAACTAACAC TCAACGCAAA AAGCATCCGC TCCACTTTGC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077375.1
CDS	137..1081
Translation	MTSCVAEEPIKKIAIFGGTHGNELTGVFLVTHWLKNGAEVHRAGLEVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEMSEDLPYEVRRAQEINHLFGPKDSDDAYDVVFVLHNTTSNMGCTLILEDSRNDFLIQMFHYIKTCMAPLPCSVYLIEHPSLKYATTRSIAKYPVGIEVGPQHHGVLRADILDQMRRMLKHALDFIQRFNEGKEFPPCAIDVYKIMEKVDYPRNESGDVAAVIHPNLQLQDQDWKPLHPGDPVFVSLDGKVIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRSTLH

Target ORF information:

RefSeq Version	NM_024399.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aspartoacylase (Aspa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024399.1

ORF Insert Sequence:

ATGACTTCTT GTGTTGCTGA AGAACCTATT AAAAAGATTG CCATCTTCGG AGGGACTCAT 
GGAAATGAAC TGACTGGAGT GTTTCTAGTT ACTCACTGGC TAAAGAATGG CGCTGAAGTT 
CACAGAGCAG GGCTGGAAGT GAAGCCATTC ATTACCAACC CAAGAGCGGT GGAGAAGTGC 
ACCAGATACA TTGACTGTGA CCTGAACCGT GTTTTTGACC TTGAAAATCT TAGCAAAGAG 
ATGTCTGAAG ATTTGCCGTA TGAAGTTAGA AGGGCTCAAG AAATAAATCA CTTATTTGGT 
CCAAAAGATA GTGATGATGC CTACGATGTC GTTTTTGTCC TTCATAACAC TACTTCTAAC 
ATGGGTTGCA CTCTTATTCT TGAGGATTCC AGGAATGACT TTTTAATCCA GATGTTTCAC 
TATATTAAGA CCTGCATGGC TCCATTACCC TGCTCTGTTT ACCTCATCGA GCATCCTTCC 
CTCAAATATG CAACCACTCG TTCCATTGCC AAGTATCCTG TTGGTATAGA AGTTGGTCCT 
CAGCATCACG GTGTTCTGAG AGCTGATATT TTAGACCAAA TGAGAAGAAT GCTCAAGCAC 
GCTCTTGATT TTATACAGCG TTTCAATGAA GGTAAAGAAT TTCCCCCCTG TGCTATTGAT 
GTCTATAAAA TAATGGAGAA AGTTGATTAT CCAAGGAACG AAAGTGGAGA CGTGGCTGCT 
GTTATCCATC CTAATCTGCA GCTGCAGGAT CAAGACTGGA AACCATTGCA CCCTGGAGAT 
CCTGTGTTTG TGTCTCTTGA TGGAAAAGTT ATTCCACTGG GTGGAGACTG TACCGTGTAC 
CCAGTGTTTG TGAATGAAGC TGCATATTAT GAAAAAAAAG AAGCATTTGC AAAGACAACA 
AAACTAACAC TCAACGCAAA AAGCATCCGC TCCACTTTGC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aspa ( XM_006246817.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246879.1 Rattus norvegicus aspartoacylase (Aspa), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa33391
Clone ID Related Accession (Same CDS sequence)	XM_006246817.3
Accession Version	XM_006246817.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	aspartoacylase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246817.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTT GTGTTGCTGA AGAACCTATT AAAAAGATTG CCATCTTCGG AGGGACTCAT 
GGAAATGAAC TGACTGGAGT GTTTCTAGTT ACTCACTGGC TAAAGAATGG CGCTGAAGTT 
CACAGAGCAG GGCTGGAAGT GAAGCCATTC ATTACCAACC CAAGAGCGGT GGAGAAGTGC 
ACCAGATACA TTGACTGTGA CCTGAACCGT GTTTTTGACC TTGAAAATCT TAGCAAAGAG 
ATGTCTGAAG ATTTGCCGTA TGAAGTGAGA AGGGCTCAAG AAATAAATCA CTTATTTGGT 
CCAAAAAATA GTGATGATGC CTACGATGTC GTTTTTGACC TTCATAACAC TACTTCTAAC 
ATGGGTTGCA CTCTTATTCT TGAGGATTCC AGGAATGACT TTTTAATCCA GATGTTTCAC 
TATATTAAGA CCTGCATGGC TCCATTACCC TGCTCTGTTT ACCTCATCGA GCATCCTTCC 
CTCAAATATG CAACCACTCG TTCCATTGCC AAGTATCCTG TTGGTATAGA AGTTGGTCCT 
CAGCCTCACG GTGTTCTGAG AGCTGATATT TTAGACCAAA TGAGAAGAAT GCTCAAGCAC 
GCTCTTGATT TTATACAGCG TTTCAATGAA GGTAAAGAAT TTCCCCCCTG TGCTATTGAT 
GTCTATAAAA TAATGGAGAA AGTTGATTAT CCAAGGAACG AAAGTGGAGA CGTGGCTGCT 
GTTATCCATC CTAATCTGCA GGAGGAAGTC ACAAGGGTTA GGGATGGACC TTCAAGGACT 
GAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246879.1
CDS	182..970
Translation	MTSCVAEEPIKKIAIFGGTHGNELTGVFLVTHWLKNGAEVHRAGLEVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEMSEDLPYEVRRAQEINHLFGPKNSDDAYDVVFDLHNTTSNMGCTLILEDSRNDFLIQMFHYIKTCMAPLPCSVYLIEHPSLKYATTRSIAKYPVGIEVGPQPHGVLRADILDQMRRMLKHALDFIQRFNEGKEFPPCAIDVYKIMEKVDYPRNESGDVAAVIHPNLQEEVTRVRDGPSRTER

Target ORF information:

RefSeq Version	XM_006246817.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aspartoacylase (Aspa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246817.3

ORF Insert Sequence:

ATGACTTCTT GTGTTGCTGA AGAACCTATT AAAAAGATTG CCATCTTCGG AGGGACTCAT 
GGAAATGAAC TGACTGGAGT GTTTCTAGTT ACTCACTGGC TAAAGAATGG CGCTGAAGTT 
CACAGAGCAG GGCTGGAAGT GAAGCCATTC ATTACCAACC CAAGAGCGGT GGAGAAGTGC 
ACCAGATACA TTGACTGTGA CCTGAACCGT GTTTTTGACC TTGAAAATCT TAGCAAAGAG 
ATGTCTGAAG ATTTGCCGTA TGAAGTGAGA AGGGCTCAAG AAATAAATCA CTTATTTGGT 
CCAAAAAATA GTGATGATGC CTACGATGTC GTTTTTGACC TTCATAACAC TACTTCTAAC 
ATGGGTTGCA CTCTTATTCT TGAGGATTCC AGGAATGACT TTTTAATCCA GATGTTTCAC 
TATATTAAGA CCTGCATGGC TCCATTACCC TGCTCTGTTT ACCTCATCGA GCATCCTTCC 
CTCAAATATG CAACCACTCG TTCCATTGCC AAGTATCCTG TTGGTATAGA AGTTGGTCCT 
CAGCCTCACG GTGTTCTGAG AGCTGATATT TTAGACCAAA TGAGAAGAAT GCTCAAGCAC 
GCTCTTGATT TTATACAGCG TTTCAATGAA GGTAAAGAAT TTCCCCCCTG TGCTATTGAT 
GTCTATAAAA TAATGGAGAA AGTTGATTAT CCAAGGAACG AAAGTGGAGA CGTGGCTGCT 
GTTATCCATC CTAATCTGCA GGAGGAAGTC ACAAGGGTTA GGGATGGACC TTCAAGGACT 
GAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aspa ( XM_006246818.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246880.1 Rattus norvegicus aspartoacylase (Aspa), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa33392
Clone ID Related Accession (Same CDS sequence)	XM_006246818.3
Accession Version	XM_006246818.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	aspartoacylase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246818.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTT GTGTTGCTGA AGAACCTATT AAAAAGATTG CCATCTTCGG AGGGACTCAT 
GGAAATGAAC TGACTGGAGT GTTTCTAGTT ACTCACTGGC TAAAGAATGG CGCTGAAGTT 
CACAGAGCAG GGCTGGAAGT GAAGCCATTC ATTACCAACC CAAGAGCGGT GGAGAAGTGC 
ACCAGATACA TTGACTGTGA CCTGAACCGT GTTTTTGACC TTGAAAATCT TAGCAAAGAG 
ATGTCTGAAG ATTTGCCGTA TGAAGTGAGA AGGGCTCAAG AAATAAATCA CTTATTTGGT 
CCAAAAAATA GTGATGATGC CTACGATGTC GTTTTTGACC TTCATAACAC TACTTCTAAC 
ATGGGTTGCA CTCTTATTCT TGAGGATTCC AGGAATGACT TTTTAATCCA GATGTTTCAC 
TATATTAAGA CCTGCATGGC TCCATTACCC TGCTCTGTTT ACCTCATCGA GCATCCTTCC 
CTCAAATATG CAACCACTCG TTCCATTGCC AAGTATCCTG TTGGTATAGA AGTTGGTCCT 
CAGCCTCACG GTGTTCTGAG AGCTGATATT TTAGACCAAA TGAGAAGAAT GCTCAAGCAC 
GCTCTTGATT TTATACAGCG TTTCAATGAA GGTAAAGAAT TTCCCCCCTG TGCTATTGAT 
GTCTATAAAA TAATGGAGAA AGTTGATTAT CCAAGGAACG AAAGTGGAGA CGTGGCTGCT 
GTTATCCATC CTAATCTGCA GGATGCTCAT ACCCCTTCCC TCCTCTCTAA AGCCACAGCA 
GTCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246880.1
CDS	182..970
Translation	MTSCVAEEPIKKIAIFGGTHGNELTGVFLVTHWLKNGAEVHRAGLEVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEMSEDLPYEVRRAQEINHLFGPKNSDDAYDVVFDLHNTTSNMGCTLILEDSRNDFLIQMFHYIKTCMAPLPCSVYLIEHPSLKYATTRSIAKYPVGIEVGPQPHGVLRADILDQMRRMLKHALDFIQRFNEGKEFPPCAIDVYKIMEKVDYPRNESGDVAAVIHPNLQDAHTPSLLSKATAVM

Target ORF information:

RefSeq Version	XM_006246818.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aspartoacylase (Aspa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246818.3

ORF Insert Sequence:

ATGACTTCTT GTGTTGCTGA AGAACCTATT AAAAAGATTG CCATCTTCGG AGGGACTCAT 
GGAAATGAAC TGACTGGAGT GTTTCTAGTT ACTCACTGGC TAAAGAATGG CGCTGAAGTT 
CACAGAGCAG GGCTGGAAGT GAAGCCATTC ATTACCAACC CAAGAGCGGT GGAGAAGTGC 
ACCAGATACA TTGACTGTGA CCTGAACCGT GTTTTTGACC TTGAAAATCT TAGCAAAGAG 
ATGTCTGAAG ATTTGCCGTA TGAAGTGAGA AGGGCTCAAG AAATAAATCA CTTATTTGGT 
CCAAAAAATA GTGATGATGC CTACGATGTC GTTTTTGACC TTCATAACAC TACTTCTAAC 
ATGGGTTGCA CTCTTATTCT TGAGGATTCC AGGAATGACT TTTTAATCCA GATGTTTCAC 
TATATTAAGA CCTGCATGGC TCCATTACCC TGCTCTGTTT ACCTCATCGA GCATCCTTCC 
CTCAAATATG CAACCACTCG TTCCATTGCC AAGTATCCTG TTGGTATAGA AGTTGGTCCT 
CAGCCTCACG GTGTTCTGAG AGCTGATATT TTAGACCAAA TGAGAAGAAT GCTCAAGCAC 
GCTCTTGATT TTATACAGCG TTTCAATGAA GGTAAAGAAT TTCCCCCCTG TGCTATTGAT 
GTCTATAAAA TAATGGAGAA AGTTGATTAT CCAAGGAACG AAAGTGGAGA CGTGGCTGCT 
GTTATCCATC CTAATCTGCA GGATGCTCAT ACCCCTTCCC TCCTCTCTAA AGCCACAGCA 
GTCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aspa ( XM_017597543.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453032.1 Rattus norvegicus aspartoacylase (Aspa), transcript variant X2, mRNA.

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CloneID	ORa57491
Clone ID Related Accession (Same CDS sequence)	XM_017597543.1
Accession Version	XM_017597543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	aspartoacylase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTT GTGTTGCTGA AGAACCTATT AAAAAGATTG CCATCTTCGG AGGGACTCAT 
GGAAATGAAC TGACTGGAGT GTTTCTAGTT ACTCACTGGC TAAAGAATGG CGCTGAAGTT 
CACAGAGCAG GGCTGGAAGT GAAGCCATTC ATTACCAACC CAAGAGCGGT GGAGAAGTGC 
ACCAGATACA TTGACTGTGA CCTGAACCGT GTTTTTGACC TTGAAAATCT TAGCAAAGAG 
ATGTCTGAAG ATTTGCCGTA TGAAGTGAGA AGGGCTCAAG AAATAAATCA CTTATTTGGT 
CCAAAAAATA GTGATGATGC CTACGATGTC GTTTTTGACC TTCATAACAC TACTTCTAAC 
ATGGGTTGCA CTCTTATTCT TGAGGATTCC AGGAATGACT TTTTAATCCA GATGTTTCAC 
TATATTAAGA CCTGCATGGC TCCATTACCC TGCTCTGTTT ACCTCATCGA GCATCCTTCC 
CTCAAATATG CAACCACTCG TTCCATTGCC AAGTATCCTG TTGGTAAAGA ATTTCCCCCC 
TGTGCTATTG ATGTCTATAA AATAATGGAG AAAGTTGATT ATCCAAGGAA CGAAAGTGGA 
GACGTGGCTG CTGTTATCCA TCCTAATCTG CAGGATCAAG ACTGGAAACC ATTGCACCCT 
GGAGATCCTG TGTTTGTGTC TCTTGATGGA AAAGTTATTC CACTGGGTGG AGACTGTACC 
GTGTACCCAG TGTTTGTGAA TGAAGCTGCA TATTATGAAA AAAAAGAAGC ATTTGCAAAG 
ACAACAAAAC TAACACTCAA CGCAAAAAGC ATCCGCTCCA CTTTGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453032.1
CDS	183..1013
Translation	MTSCVAEEPIKKIAIFGGTHGNELTGVFLVTHWLKNGAEVHRAGLEVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEMSEDLPYEVRRAQEINHLFGPKNSDDAYDVVFDLHNTTSNMGCTLILEDSRNDFLIQMFHYIKTCMAPLPCSVYLIEHPSLKYATTRSIAKYPVGKEFPPCAIDVYKIMEKVDYPRNESGDVAAVIHPNLQDQDWKPLHPGDPVFVSLDGKVIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRSTLH

Target ORF information:

RefSeq Version	XM_017597543.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aspartoacylase (Aspa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597543.1

ORF Insert Sequence:

ATGACTTCTT GTGTTGCTGA AGAACCTATT AAAAAGATTG CCATCTTCGG AGGGACTCAT 
GGAAATGAAC TGACTGGAGT GTTTCTAGTT ACTCACTGGC TAAAGAATGG CGCTGAAGTT 
CACAGAGCAG GGCTGGAAGT GAAGCCATTC ATTACCAACC CAAGAGCGGT GGAGAAGTGC 
ACCAGATACA TTGACTGTGA CCTGAACCGT GTTTTTGACC TTGAAAATCT TAGCAAAGAG 
ATGTCTGAAG ATTTGCCGTA TGAAGTGAGA AGGGCTCAAG AAATAAATCA CTTATTTGGT 
CCAAAAAATA GTGATGATGC CTACGATGTC GTTTTTGACC TTCATAACAC TACTTCTAAC 
ATGGGTTGCA CTCTTATTCT TGAGGATTCC AGGAATGACT TTTTAATCCA GATGTTTCAC 
TATATTAAGA CCTGCATGGC TCCATTACCC TGCTCTGTTT ACCTCATCGA GCATCCTTCC 
CTCAAATATG CAACCACTCG TTCCATTGCC AAGTATCCTG TTGGTAAAGA ATTTCCCCCC 
TGTGCTATTG ATGTCTATAA AATAATGGAG AAAGTTGATT ATCCAAGGAA CGAAAGTGGA 
GACGTGGCTG CTGTTATCCA TCCTAATCTG CAGGATCAAG ACTGGAAACC ATTGCACCCT 
GGAGATCCTG TGTTTGTGTC TCTTGATGGA AAAGTTATTC CACTGGGTGG AGACTGTACC 
GTGTACCCAG TGTTTGTGAA TGAAGCTGCA TATTATGAAA AAAAAGAAGC ATTTGCAAAG 
ACAACAAAAC TAACACTCAA CGCAAAAAGC ATCCGCTCCA CTTTGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Involvement of aspartoacylase in tremor expression in rats.
Experimental animals65(3)293-301(2016 Jul)
Nishitani A,Tanaka M,Shimizu S,Kunisawa N,Yokoe M,Yoshida Y,Suzuki T,Sakuma T,Yamamoto T,Kuwamura M,Takenaka S,Ohno Y,Kuramoto T

The molecular mechanisms affecting N-acetylaspartate homeostasis following experimental graded traumatic brain injury.
Molecular medicine (Cambridge, Mass.)20147-57(2014 Mar)
Di Pietro V,Amorini AM,Tavazzi B,Vagnozzi R,Logan A,Lazzarino G,Signoretti S,Lazzarino G,Belli A

Expression and localization of myosin-1d in the developing nervous system.
Brain research14409-22(2012 Feb)
Benesh AE,Fleming JT,Chiang C,Carter BD,Tyska MJ

Extensive aspartoacylase expression in the rat central nervous system.
Glia59(10)1414-34(2011 Oct)
Moffett JR,Arun P,Ariyannur PS,Garbern JY,Jacobowitz DM,Namboodiri AM

Structure of aspartoacylase, the brain enzyme impaired in Canavan disease.
Proceedings of the National Academy of Sciences of the United States of America104(2)456-61(2007 Jan)
Bitto E,Bingman CA,Wesenberg GE,McCoy JG,Phillips GN Jr

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Aspa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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