Aco2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aco2
Entrez Gene ID	79250
Full Name	aconitase 2
General protein information	Preferred Names aconitase 2 Names aconitate hydratase, mitochondrial aconitase 2, mitochondrial citrate hydro-lyase mitochondrial aconitase (nuclear aco2 gene)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34

mRNA and Protein(s)

mRNA	Protein	Name
NM_024398.2	NP_077374.2	aconitate hydratase, mitochondrial precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Aco2	NP_542364.1
Caenorhabditis elegans (roundworm)	aco-2	NP_741235.1
Saccharomyces cerevisiae (baker's yeast)	ACO1	NP_013407.1
Canis lupus familiaris (dog)	ACO2	XP_849166.1
Gallus gallus (chicken)	ACO2	NP_989519.1
	ACO2	XP_001105023.1
Drosophila melanogaster (fruit fly)	Acon	NP_001163030.1
Bos taurus (cattle)	ACO2	NP_776402.1
Rattus norvegicus (Norway rat)	Aco2	NP_077374.2
Danio rerio (zebrafish)	aco2	NP_944590.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007852	XP_317642.4
Eremothecium gossypii	AGOS_ADL032W	NP_984065.1
Schizosaccharomyces pombe (fission yeast)	SPAC24C9.06c	NP_594031.2
Magnaporthe oryzae (rice blast fungus)	MGG_03521	XP_003716398.1
Neurospora crassa	NCU02366	XP_959787.1
Xenopus tropicalis (tropical clawed frog)	aco2	NP_001011445.1
Homo sapiens (human)	ACO2	NP_001089.1
Kluyveromyces lactis	KLLA0C17314g	XP_452974.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondria-targeted Ogg1 and aconitase-2 prevent oxidant-induced mitochondrial DNA damage in alveolar epithelial cells.
Kim SJ, Cheresh P, Williams D, Cheng Y, Ridge K, Schumacker PT, Weitzman S, Bohr VA, Kamp DW
The Journal of biological chemistry289(9)6165-76(2014 Feb)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, Le?n IR, Bak S, Mogensen M, Wrzesinski K, H?jlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

Social isolation in rats inhibits oxidative metabolism, decreases the content of mitochondrial K-Ras and activates mitochondrial hexokinase.
Zhuravliova E, Barbakadze T, Zaalishvili E, Chipashvili M, Koshoridze N, Mikeladze D
Behavioural brain research205(2)377-83(2009 Dec)

Regulation of mitochondrial aconitase by phosphorylation in diabetic rat heart.
Lin G, Brownsey RW, MacLeod KM
Cellular and molecular life sciences : CMLS66(5)919-32(2009 Mar)

Proteolipid protein is required for transport of sirtuin 2 into CNS myelin.
Werner HB, Kuhlmann K, Shen S, Uecker M, Schardt A, Dimova K, Orfaniotou F, Dhaunchak A, Brinkmann BG, M?bius W, Guarente L, Casaccia-Bonnefil P, Jahn O, Nave KA
The Journal of neuroscience : the official journal of the Society for Neuroscience27(29)7717-30(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ogg1 chaperoning of Aco-2 in preventing oxidant-mediated mtDNA damage and apoptosis may afford an innovative target for the molecular events underlying oxidant-induced toxicity.
Title: Mitochondria-targeted Ogg1 and aconitase-2 prevent oxidant-induced mitochondrial DNA damage in alveolar epithelial cells.

These results demonstrate the regulation of mitochondrial aconitase activity by protein kinase C-dependent phosphorylation.
Title: Regulation of mitochondrial aconitase by phosphorylation in diabetic rat heart.

Data show that the generation of potent reactive oxygen species (hydroxyl radicals) in cardiac H9c2 cells is attenuated by heat shock treatment.
Title: Heat shock-induced attenuation of hydroxyl radical generation and mitochondrial aconitase activity in cardiac H9c2 cells.

Data suggest that an increase of transglutaminase 2 activity in Huntington disease caudate may contribute to mitochondrial dysfunction by incorporating aconitase into inactive polymers.
Title: Mitochondrial aconitase is a transglutaminase 2 substrate: transglutamination is a probable mechanism contributing to high-molecular-weight aggregates of aconitase and loss of aconitase activity in Huntington disease brain.

frataxin is an iron chaperone protein that protects the aconitase [4Fe-4S]2+ cluster from disassembly and promotes enzyme reactivation
Title: Frataxin acts as an iron chaperone protein to modulate mitochondrial aconitase activity.

The following Aco2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aco2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13126	NM_024398.2 Latest version!	Rattus norvegicus aconitase 2 (Aco2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aco2 ( NM_024398.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077374.2 Rattus norvegicus aconitase 2 (Aco2), mRNA.

Price & Availability↑

CloneID	ORa13126
Clone ID Related Accession (Same CDS sequence)	NM_024398.2
Accession Version	NM_024398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	aconitate hydratase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061999.1. On Jan 1, 2004 this sequence version replaced NM_024398.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061999.1, AJ243266.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGCCTT ACAGCCTACT GGTCACCCGG CTGCAGAAGG CCCTGGGTGT GCGGCAGTAC 
CATGTGGCCT CTGCCCTGTG CCAACGGGCC AAGGTGGCCA TGAGCCACTT TGAGCCCAGT 
GAGTACATCC GATATGACCT GCTAGAGAAG AACATTAACA TTGTCCGTAA ACGGTTGAAC 
CGGCCTCTTA CTCTCTCGGA GAAGATTGTG TATGGACACC TGGATGACCC AGCCAACCAG 
GAGATCGAGC GGGGAAAGAC ATACCTGCGT TTACGGCCCG ACCGGGTGGC CATGCAGGAT 
GCAACAGCCC AGATGGCTAT GCTACAGTTT ATTAGCAGTG GGCTGCCCAA GGTGGCTGTG 
CCATCAACCA TCCACTGTGA TCATCTGATT GAGGCCCAGC TTGGGGGTGA GAAAGACCTA 
CGCCGGGCCA AGGACATAAA CCAGGAAGTA TATAATTTCC TGGCAACTGC CGGTGCCAAG 
TATGGTGTGG GCTTCTGGAG GCCTGGATCT GGAATCATTC ACCAGATCAT TCTAGAAAAC 
TATGCATACC CTGGAGTTCT TCTGATTGGC ACTGACTCCC ACACCCCCAA TGGGGGTGGC 
CTGGGAGGTA TCTGCATTGG AGTAGGGGGT GCTGATGCTG TGGATGTCAT GGCTGGGATC 
CCCTGGGAGC TGAAGTGTCC CAAGGTGATT GGCGTGAAGC TGACGGGCAC CCTCTCTGGT 
TGGACCTCAC CCAAAGATGT GATCCTGAAA GTGGCCGGCA TCCTCACAGT GAAAGGTGGC 
ACAGGTGCCA TTGTGGAATA CCATGGACCT GGTGTCGACT CCATCTCCTG CACTGGCATG 
GCAACAATTT GCAACATGGG TGCAGAAATT GGGGCCACTA CATCAGTGTT CCCCTACAAC 
CACAGGATGA AAAAGTACCT GAGCAAGACA GGCCGAGCAG ACATTGCCAA CCTAGCAGAA 
GAATTCAAGG ATCACTTAGT GCCTGACCCT GGCTGCCAGT ATGACCAAGT GATTGAAATT 
AACCTCAATG AGCTAAAGCC ACATATCAAT GGACCCTTTA CCCCTGACTT GGCTCACCCA 
GTGGCAGATG TGGGCACTGT GGCAGAGAAG GAAGGGTGGC CTCTGGACAT CAGAGTAGGT 
TTGATTGGCA GCTGCACCAA TTCAAGCTAC GAGGACATGG GACGATCAGC AGCTGTGGCC 
AAGCAAGCCC TGGCGCACGG ACTCAAGTGC AAGTCTCAGT TCACCATCAC CCCAGGCTCT 
GAGCAGATCC GAGCCACTAT CGAGCGGGAT GGCTATGCAC AGATCCTAAG GGATGTGGGT 
GGAATCGTTT TGGCCAATGC CTGTGGCCCT TGCATCGGCC AGTGGGACAG GAAAGACATC 
AAGAAGGGGG AGAAGAATAC AATCGTCACC TCCTACAACA GGAACTTCAC AGGCCGTAAT 
GATGCAAATC CTGAGACCCA TGCCTTTGTC ACATCCCCAG AGATTGTCAC AGCCCTGGCC 
ATTGCGGGAA CCCTTAAGTT CAACCCAGAA ACTGACTTCT TAACAGGCAA GGATGGCAAG 
AAGTTCAAGT TGGAGGCTCC AGATGCAGAT GAGCTTCCCC GATCGGACTT TGATCCAGGG 
CAGGACACAT ACCAGCACCC CCCTAAGGAC AGCAGTGGCC AGCGGGTGGA TGTGAGCCCT 
ACCAGCCAGC GCCTGCAGCT CCTGGAACCT TTTGACAAAT GGGATGGCAA AGACCTAGAG 
GACCTGCAAA TCCTTATCAA GGTCAAAGGG AAGTGCACCA CAGACCACAT TTCTGCTGCC 
GGCCCCTGGC TCAAGTTCCG TGGGCATCTG GATAACATCT CTAATAACCT GCTTATCGGT 
GCCATCAACA TCGAAAATGG CAAAGCCAAT TCTGTGCGCA ATGCTGTCAC CCAGGAGTTT 
GGCCCTGTAC CTGACACTGC TCGCTACTAC AAGAAACATG GCATCAGGTG GGTGGTGATT 
GGAGATGAGA ACTACGGTGA AGGCTCAAGC CGGGAGCACG CAGCACTGGA GCCTCGCCAT 
CTGGGGGGCC GAGCCATCAT CACCAAGAGC TTCGCAAGGA TCCATGAAAC CAATCTAAAG 
AAGCAGGGCT TGCTGCCCCT CACCTTTGCT GACCCCTCTG ACTACAACAA GATTCATCCA 
GTGGACAAGC TGACCATTCA AGGCCTGAAG GACTTTGCCC CTGGCAAGCC TCTGAACTGC 
ATCATCAAGC ACCCCAATGG GACCCAGGAG ACCATCCTGC TGAACCACAC TTTCAACGAG 
ACACAGATCG AGTGGTTCCG TGCAGGCAGC GCCCTCAACA GGATGAAGGA GCTGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077374.2
CDS	5..2347
Translation	MAPYSLLVTRLQKALGVRQYHVASALCQRAKVAMSHFEPSEYIRYDLLEKNINIVRKRLNRPLTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGTLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADIANLAEEFKDHLVPDPGCQYDQVIEINLNELKPHINGPFTPDLAHPVADVGTVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRSDFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPSDYNKIHPVDKLTIQGLKDFAPGKPLNCIIKHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQQ

Target ORF information:

RefSeq Version	NM_024398.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aconitase 2 (Aco2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024398.2

ORF Insert Sequence:

ATGGCGCCTT ACAGCCTACT GGTCACCCGG CTGCAGAAGG CCCTGGGTGT GCGGCAGTAC 
CATGTGGCCT CTGCCCTGTG CCAACGGGCC AAGGTGGCCA TGAGCCACTT TGAGCCCAGT 
GAGTACATCC GATATGACCT GCTAGAGAAG AACATTAACA TTGTCCGTAA ACGGTTGAAC 
CGGCCTCTTA CTCTCTCGGA GAAGATTGTG TATGGACACC TGGATGACCC AGCCAACCAG 
GAGATCGAGC GGGGAAAGAC ATACCTGCGT TTACGGCCCG ACCGGGTGGC CATGCAGGAT 
GCAACAGCCC AGATGGCTAT GCTACAGTTT ATTAGCAGTG GGCTGCCCAA GGTGGCTGTG 
CCATCAACCA TCCACTGTGA TCATCTGATT GAGGCCCAGC TTGGGGGTGA GAAAGACCTA 
CGCCGGGCCA AGGACATAAA CCAGGAAGTA TATAATTTCC TGGCAACTGC CGGTGCCAAG 
TATGGTGTGG GCTTCTGGAG GCCTGGATCT GGAATCATTC ACCAGATCAT TCTAGAAAAC 
TATGCATACC CTGGAGTTCT TCTGATTGGC ACTGACTCCC ACACCCCCAA TGGGGGTGGC 
CTGGGAGGTA TCTGCATTGG AGTAGGGGGT GCTGATGCTG TGGATGTCAT GGCTGGGATC 
CCCTGGGAGC TGAAGTGTCC CAAGGTGATT GGCGTGAAGC TGACGGGCAC CCTCTCTGGT 
TGGACCTCAC CCAAAGATGT GATCCTGAAA GTGGCCGGCA TCCTCACAGT GAAAGGTGGC 
ACAGGTGCCA TTGTGGAATA CCATGGACCT GGTGTCGACT CCATCTCCTG CACTGGCATG 
GCAACAATTT GCAACATGGG TGCAGAAATT GGGGCCACTA CATCAGTGTT CCCCTACAAC 
CACAGGATGA AAAAGTACCT GAGCAAGACA GGCCGAGCAG ACATTGCCAA CCTAGCAGAA 
GAATTCAAGG ATCACTTAGT GCCTGACCCT GGCTGCCAGT ATGACCAAGT GATTGAAATT 
AACCTCAATG AGCTAAAGCC ACATATCAAT GGACCCTTTA CCCCTGACTT GGCTCACCCA 
GTGGCAGATG TGGGCACTGT GGCAGAGAAG GAAGGGTGGC CTCTGGACAT CAGAGTAGGT 
TTGATTGGCA GCTGCACCAA TTCAAGCTAC GAGGACATGG GACGATCAGC AGCTGTGGCC 
AAGCAAGCCC TGGCGCACGG ACTCAAGTGC AAGTCTCAGT TCACCATCAC CCCAGGCTCT 
GAGCAGATCC GAGCCACTAT CGAGCGGGAT GGCTATGCAC AGATCCTAAG GGATGTGGGT 
GGAATCGTTT TGGCCAATGC CTGTGGCCCT TGCATCGGCC AGTGGGACAG GAAAGACATC 
AAGAAGGGGG AGAAGAATAC AATCGTCACC TCCTACAACA GGAACTTCAC AGGCCGTAAT 
GATGCAAATC CTGAGACCCA TGCCTTTGTC ACATCCCCAG AGATTGTCAC AGCCCTGGCC 
ATTGCGGGAA CCCTTAAGTT CAACCCAGAA ACTGACTTCT TAACAGGCAA GGATGGCAAG 
AAGTTCAAGT TGGAGGCTCC AGATGCAGAT GAGCTTCCCC GATCGGACTT TGATCCAGGG 
CAGGACACAT ACCAGCACCC CCCTAAGGAC AGCAGTGGCC AGCGGGTGGA TGTGAGCCCT 
ACCAGCCAGC GCCTGCAGCT CCTGGAACCT TTTGACAAAT GGGATGGCAA AGACCTAGAG 
GACCTGCAAA TCCTTATCAA GGTCAAAGGG AAGTGCACCA CAGACCACAT TTCTGCTGCC 
GGCCCCTGGC TCAAGTTCCG TGGGCATCTG GATAACATCT CTAATAACCT GCTTATCGGT 
GCCATCAACA TCGAAAATGG CAAAGCCAAT TCTGTGCGCA ATGCTGTCAC CCAGGAGTTT 
GGCCCTGTAC CTGACACTGC TCGCTACTAC AAGAAACATG GCATCAGGTG GGTGGTGATT 
GGAGATGAGA ACTACGGTGA AGGCTCAAGC CGGGAGCACG CAGCACTGGA GCCTCGCCAT 
CTGGGGGGCC GAGCCATCAT CACCAAGAGC TTCGCAAGGA TCCATGAAAC CAATCTAAAG 
AAGCAGGGCT TGCTGCCCCT CACCTTTGCT GACCCCTCTG ACTACAACAA GATTCATCCA 
GTGGACAAGC TGACCATTCA AGGCCTGAAG GACTTTGCCC CTGGCAAGCC TCTGAACTGC 
ATCATCAAGC ACCCCAATGG GACCCAGGAG ACCATCCTGC TGAACCACAC TTTCAACGAG 
ACACAGATCG AGTGGTTCCG TGCAGGCAGC GCCCTCAACA GGATGAAGGA GCTGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondria-targeted Ogg1 and aconitase-2 prevent oxidant-induced mitochondrial DNA damage in alveolar epithelial cells.
The Journal of biological chemistry289(9)6165-76(2014 Feb)
Kim SJ,Cheresh P,Williams D,Cheng Y,Ridge K,Schumacker PT,Weitzman S,Bohr VA,Kamp DW

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)
Zhao X,Le?n IR,Bak S,Mogensen M,Wrzesinski K,H?jlund K,Jensen ON

Social isolation in rats inhibits oxidative metabolism, decreases the content of mitochondrial K-Ras and activates mitochondrial hexokinase.
Behavioural brain research205(2)377-83(2009 Dec)
Zhuravliova E,Barbakadze T,Zaalishvili E,Chipashvili M,Koshoridze N,Mikeladze D

Regulation of mitochondrial aconitase by phosphorylation in diabetic rat heart.
Cellular and molecular life sciences : CMLS66(5)919-32(2009 Mar)
Lin G,Brownsey RW,MacLeod KM

Proteolipid protein is required for transport of sirtuin 2 into CNS myelin.
The Journal of neuroscience : the official journal of the Society for Neuroscience27(29)7717-30(2007 Jul)
Werner HB,Kuhlmann K,Shen S,Uecker M,Schardt A,Dimova K,Orfaniotou F,Dhaunchak A,Brinkmann BG,M?bius W,Guarente L,Casaccia-Bonnefil P,Jahn O,Nave KA

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Aco2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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