Shank1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shank1
Entrez Gene ID	78957
Full Name	SH3 and multiple ankyrin repeat domains 1
Synonyms	Shank1a,Spank1,Sstrip
General protein information	Preferred Names SH3 and multiple ankyrin repeat domains 1 Names SH3 and multiple ankyrin repeat domains protein 1 GKAP/SAPAP-interacting protein SAPAP-interacting protein synamon SH3/ankyrin domain gene 1 SPANK-1 SSTR-interacting protein somatostatin receptor-interacting protein synamon
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	a scaffold protein in the post synaptic density that forms a complex with GKAP and PSD-95 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017589758.1	XP_017445247.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
XM_017589759.1	XP_017445248.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X2
XM_008759416.2	XP_008757638.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X4
XM_017589761.1	XP_017445250.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X5
XM_017589760.1	XP_017445249.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X3
NM_031751.3	NP_113939.2	SH3 and multiple ankyrin repeat domains protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SHANK1	XP_005616915.1
Xenopus tropicalis (tropical clawed frog)	shank1	XP_002938582.2
Homo sapiens (human)	SHANK1	NP_057232.2
	LOC719673	XP_002808253.1
Bos taurus (cattle)	SHANK1	XP_002695260.2
Mus musculus (house mouse)	Shank1	NP_001029287.1
Rattus norvegicus (Norway rat)	Shank1	NP_113939.2

Gene Ontology
Bibliography

Related articles in PubMed

Shank Proteins Differentially Regulate Synaptic Transmission.
Shi R, Redman P, Ghose D, Liu Y, Ren X, Ding LJ, Liu M, Jones KJ, Xu W
eNeuro4(6)(2017 Nov-Dec)

SHANK proteins limit integrin activation by directly interacting with Rap1 and?R-Ras.
Lilja J, Zacharchenko T, Georgiadou M, Jacquemet G, De Franceschi N, Peuhu E, Hamidi H, Pouwels J, Martens V, Nia FH, Beifuss M, Boeckers T, Kreienkamp HJ, Barsukov IL, Ivaska J
Nature cell biology19(4)292-305(2017 Apr)

Differential distribution of Shank and GKAP at the postsynaptic density.
Tao-Cheng JH, Yang Y, Reese TS, Dosemeci A
PloS one10(3)e0118750(2015)

Alternative polyadenylation and differential expression of Shank mRNAs in the synaptic neuropil.
Epstein I, Tushev G, Will TJ, Vlatkovic I, Cajigas IJ, Schuman EM
Philosophical transactions of the Royal Society of London. Series B, Biological sciences369(1633)20130137(2014 Jan)

SHANK1 Deletions in Males with Autism Spectrum Disorder.
Sato D, Lionel AC, Leblond CS, Prasad A, Pinto D, Walker S, O'Connor I, Russell C, Drmic IE, Hamdan FF, Michaud JL, Endris V, Roeth R, Delorme R, Huguet G, Leboyer M, Rastam M, Gillberg C, Lathrop M, Stavropoulos DJ, Anagnostou E, Weksberg R, Fombonne E, Zwaigenbaum L, Fernandez BA, Roberts W, Rappold GA, Marshall CR, Bourgeron T, Szatmari P, Scherer SW
American journal of human genetics90(5)879-87(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SHANK1 and SHANK3 act as integrin activation inhibitors by sequestering active Rap1 and R-Ras via the SPN domain and thus limiting their bioavailability at the plasma membrane.
Title: SHANK proteins limit integrin activation by directly interacting with Rap1 and?R-Ras.

Upon depolarization with high K+, neither the intensity nor distribution of label for GKAP changed, but labeling intensity for Shank at the postsynaptic density increased to ~150% of controls.
Title: Differential distribution of Shank and GKAP at the postsynaptic density.

All three Shank mRNA isoforms are differentially expressed in the synaptic neuropil.
Title: Alternative polyadenylation and differential expression of Shank mRNAs in the synaptic neuropil.

this study provides a new level of understanding of the specificity and structural plasticity of the shank PDZ domain.
Title: The structural flexibility of the shank1 PDZ domain is important for its binding to different ligands.

Despite the fact that the betaPIX coiled-coil domain exposes three PDZ binding motifs in the C-termini, the betaPIX trimer associates with a single Shank PDZ.
Title: Structural basis for asymmetric association of the betaPIX coiled coil and shank PDZ.

The following Shank1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shank1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa22785	XM_008759416.2 Latest version!	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa22786	XM_017589761.1 Latest version!	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa56860	XM_017589758.1 Latest version!	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa08651	NM_031751.3 Latest version!	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa22781	XM_017589759.1 Latest version!	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa22782	XM_017589760.1 Latest version!	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shank1 ( XM_008759416.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757638.1 Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa22785
Clone ID Related Accession (Same CDS sequence)	XM_008759416.2
Accession Version	XM_008759416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759416.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTCT GATCGATGGG ATTGACTCAG GGAGTGATTA CATCATCAAG 
GAGAAGACAG TCTTACTGCA GAAGAAGGAC AGTGAGGGGT TCGGGTTCGT GCTCCGGGGG 
GCCAAGGCGC AGACCCCCAT CGAGGAATTC ACCCCCACCC CGGCCTTCCC GGCGCTGCAG 
TACCTCGAGT CTGTGGACGA GGGTGGCGTG GCATGGCGGG CTGGACTACG GATGGGAGAC 
TTCCTCATCG AGGTGAATGG ACAGAATGTA GTGAAGGTTG GCCACCGCCA GGTGGTCAAC 
ATGATCCGAC AGGGAGGCAA CACACTGATG GTCAAGGTGG TGATGGTGAC CAGACACCCA 
GACATGGACG AGGCTGTCCA CAAGAAAGCA TCCCAGCAGG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCC TGCGTTCCAA GTCTATGACC TCAGAGCTGG AGGAAATGGT CTCCCCCTGG 
AAGAAGAAAA TTGAATACGA GCAACAGCCC GCGGCAGTGC CCAGCATGGA GAAAAAGAGG 
ACTGTGTATC AGATGGCACT CAACAAACTG GATGAGATCT TGGCCGCTGC TCAGCAGACC 
ATCAGCGCAA GTGAGAGTCC TGGGCCTGGT GGCCTGGCAT CCCTGGGCAA GCACCGACCC 
AAAGGATTCT TTGCCACTGA GTCGAGCTTT GATCCTCACC ACCGCTCCCA GCCAAGTTAT 
GACCGGCCTT CTTTCCTGCC TCCTGGACCC GGCCTTATGC TCCGGCAGAA ATCTATCGGG 
GCTGCGGAAG ACGACAGACC CTACCTAGCA CCCCCAGCCA TGAAATTCAG TCGTAGCCTG 
TCAGTCCCCG GTTCAGAGGA CATACCCCCA CCACCCACTA CATCACCACC AGAGCCGCCC 
TATAGCACAC CTCCGGCCCC CTCCTCCTCC GGCCGCCTCA CTCCCTCGCC TCGGGGAGGG 
CCCTTTAACC CCAGTTCTGG GGGTCCCCTT CCCGCCTCCT CCCCTTCATC TTTCGATGGG 
CCATCTCCTC CTGACACCCG TGGTGGGGGC CGAGAAAAGA GCCTATATCA CAGCGCAGCC 
CTGCCCCCTG CCCACCACCA TCCACCTCAT CACCACCATC ATCACGCGCC ACCTCCCCAG 
CCCCACCATC ACCATGCACA TCCTCCTCAC CCACCGGAGA TGGAGACAGG AGGTTCCCCC 
GACGATCCCC CACCTCGATT GGCCCTGGGA CCCCAGCCCA GCCTTCGAGG CTGGCGAGGT 
GGAGGACCCA GCCCGACCTC GGGGGCCCCA TCACCTTCCC ACCACAGTAG CAGCGGAGGC 
AGCAGCGGCC CTACTCAGGC TCCCGCCCTG CGCTACTTCC AACTGCCCCC GAGGGCGGCC 
AGTGCGGCTA TGTATGTACC AGCACGTTCC GGCCGCGGTC GCAAGGGCCC ACTGGTCAAG 
CAGACCAAAG TGGAAGGCGA GCCCCAAAAG GGCAGCATCC CTTCCGCGTC CTCTCCAACA 
TCCCCGGCGT TGCCTCGGTC TGAACCACCT CCGGCCGGGC CGTCGGAGAA AAATTCCATC 
CCTATCCCGA CCATCATCAT CAAAGCCCCA TCTACCAGTA GCAGCGGCCG CAGCAGTCAG 
GGAAGCAGCA CAGAGGCTGA GCCCCCCACC CAGCCCGATG GCGCGGGCGG CGGCGGATCC 
TCGCCTAGCC CTGCACCTGC CACGTCCCCT GTGCCGCCAT CCCCGTCGCC CGTGCCCACT 
CCCGCGTCAC CCAGCGGCCC GGCCACCCTG GATTTCACTA GCCAGTTTGG AGCAGCCCTA 
GTAGGCGCGG CGCGGAGGGA GGGAGGCTGG CAGAACGAAG CCCGGCGGCG GTCCACGCTG 
TTCCTGTCCA CCGACGCAGG AGATGAGGAT GGAGGCGACA GCGGGCTGGG CCCGGGGGGG 
CCCCCGGGTC CCCGGCTGCG CCATTCCAAA TCCATTGACG AAGGCATGTT CTCCGCAGAG 
CCGTACCTCC GGCTGGAGTC AGGGGGCAGC AGTGGGGGCT ACGGGGCCTA CGCAGCGGGC 
AGCCGAGCCT ACGGGGGCAG CGGGAGCAGC AGCGCCTTCA CCAGCTTCCT CCCCCCGCGG 
CCCCTGGTGC ATCCGCTGAC CGGCAAAGCC TTGGATCCCG CCTCTCCGCT GGGGTTGGCC 
TTGGCCGCCC GGGAGCGGGC CTTGAAGGAG TCCTCGGAGG GTGGGGGCAC CCCTCAGCCG 
CCCCCGAGAC CTCCATCACC ACGCTACGAC GCCCCACCGC CTACCCTTCA CCACCACTCG 
CCCCATTCAC CCCATTCGCC ACATGCGCGT CACGAGCCAG TGTTGCGCCT CTGGGGGGAC 
CCCGCGCGCC GGGAGCTGGG GTACCGGGCA GGCCTAGGGA GTCAGGAGAA GGCCCTTACC 
GCCAGTCCAC CTGCAGCTCG CCGCTCCCTA CTGCACCGTC TGCCCCCGAC GGCTCCCGGG 
GTCGGGCCGC TCCTGCTGCA GCTGGGGCCG GAGCCCCCAA CTCCCCATCC TGGGGTGAGC 
AAGGCCTGGC GCACAGCAGC CCCGGAGGAG CCGGAGCGGC TGCCGCTGCA CGTGCGATTC 
CTTGAGAATT GCCAAGCCCG GCCTCCCCCA GCTGGCACGA GAGGGTCCTC TACTGAAGAT 
GGGCCTGGGG TCCCGCCTCC CAGCCCTCGC AGAGTCTTGC CAACCTCTCC CACCTCCCCA 
CGAGGCAACG AGGAGAACGG CCTTCCGCTA CTGGTCCTGC CACCGCCAGC CCCTTCGGTG 
GATGTGGACG ACGGCGAGTT CTTGTTTGCG GAGCCGCTGC CTCCACCCCT GGAGTTTTCC 
AATAGTTTCG AAAAGCCAGA GTCGCCTCTC ACACCCGGGC CTCCTCACCC ACTACCTGAC 
CCTCCCTCCC CGGCTACCCC TCTACCCGCA GCCCCACCTC CCGCTGTAGC CGCAGCCCCT 
CCCACCTTGG ACTCCACAGC ATCCAGCCTG ACCTCTTACG ACAGCGAAGT GGCCACACTG 
ACCCAGGGGG CTCCCGCGGC TCCTGGGGAT CCTCCAGCTC CAGGCCCGCC GGCCCCGGCA 
GCCCCAGCTC CCCCAGCACC GCAGCCAGGC CCTGATCCTC CACCTGGCAC AGATTCTGGA 
ATTGAGGAGG TGGACAGTCG GAGTAGCAGT GATCACCCGC TGGAGACCAT CAGCAGCGCA 
TCCACACTCA GCAGCTTGTC CGCCGAAGGA GGAGGCAATA CAGGGGGCGT GGCTGGGGGC 
GGGGCCGGGG TGGCCAGTGG GACGGAGCTC CTGGACACCT ACGTTGCCTA CCTGGATGGC 
CAGGCCTTCG GGGGCAGTGG AACTCCTGGC CCACCATATC CTCCCCAGCT CATGACTCCT 
TCTAAGCTCC GGGGCAGGGC GCTTGGGACT AGTGGAAATT TAAGACCTGG TCCCAGTGGG 
GGACTCCGAG ATCCTGTCAC CCCCACCAGC CCCACTGTCT CTGTGACCGG AGCTGGAACA 
GATGGGCTAC TGGCCTTGAG TGCTTGCCCT GGACCCTCAA CGGCAGGTGT GGCAGGGGGT 
CCCGTGGCAG TTGAGCCAGA AGTCCCGCCT GTACCTTTGC CCGCTGCCTC ATCCCTGCCT 
CGGAAGCTAT TACCGTGGGA GGAGGGTCCG GGACCACCAC CACCACCCCT GCCAGGGCCC 
CTGTCCCAGC CTCAGGCCTC TGCCTTGGCC ACGGTCAAAG CCAGCATCAT CAGCGAACTC 
AGCTCCAAGC TCCAGCAGTT TGGTGGGTCC TCTACCGCAG GAGGAGCTCT GCCTTGGGCT 
CGGGGAGGCA GCGGGGGCAG CACAGACAGC CACCACGGAG GAGCCAGTTA CATCCCAGAG 
AGGACGTCCT CCCTGCAGCG GCAGAGGAAA CGGACCCCTA AGAAGCACAC TTTCAGCTCC 
ATGGTCACCA TAACCCATGG CCCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757638.1
CDS	102..5951
Translation	MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPQGTRGRGSGAPGNLASTRGLQGRSMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKETCARILLYRGANKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARRRGPPGAGLTVPPALLRANSDTSMALPDWMVFSAPGASSSGTPGPTSGPQGQSQPSAPSTKLSSGTLRSASSPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPRDGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQAQGEGEISLSKGEKIKVLSIGEGGFWEGQVKGRVGWFPSDCLEEVANRSQEGRQESRSDKAKRLFRHYTVGSYDSFDAPSLIDGIDSGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKASQQAKRLPPPAISLRSKSMTSELEEMVSPWKKKIEYEQQPAAVPSMEKKRTVYQMALNKLDEILAAAQQTISASESPGPGGLASLGKHRPKGFFATESSFDPHHRSQPSYDRPSFLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPSSGGPLPASSPSSFDGPSPPDTRGGGREKSLYHSAALPPAHHHPPHHHHHHAPPPQPHHHHAHPPHPPEMETGGSPDDPPPRLALGPQPSLRGWRGGGPSPTSGAPSPSHHSSSGGSSGPTQAPALRYFQLPPRAASAAMYVPARSGRGRKGPLVKQTKVEGEPQKGSIPSASSPTSPALPRSEPPPAGPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQPDGAGGGGSSPSPAPATSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDAGDEDGGDSGLGPGGPPGPRLRHSKSIDEGMFSAEPYLRLESGGSSGGYGAYAAGSRAYGGSGSSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGTPQPPPRPPSPRYDAPPPTLHHHSPHSPHSPHARHEPVLRLWGDPARRELGYRAGLGSQEKALTASPPAARRSLLHRLPPTAPGVGPLLLQLGPEPPTPHPGVSKAWRTAAPEEPERLPLHVRFLENCQARPPPAGTRGSSTEDGPGVPPPSPRRVLPTSPTSPRGNEENGLPLLVLPPPAPSVDVDDGEFLFAEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPSPATPLPAAPPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPAPGPPAPAAPAPPAPQPGPDPPPGTDSGIEEVDSRSSSDHPLETISSASTLSSLSAEGGGNTGGVAGGGAGVASGTELLDTYVAYLDGQAFGGSGTPGPPYPPQLMTPSKLRGRALGTSGNLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLALSACPGPSTAGVAGGPVAVEPEVPPVPLPAASSLPRKLLPWEEGPGPPPPPLPGPLSQPQASALATVKASIISELSSKLQQFGGSSTAGGALPWARGGSGGSTDSHHGGASYIPERTSSLQRQRKRTPKKHTFSSMVTITHGPR

Target ORF information:

RefSeq Version	XM_008759416.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759416.2

ORF Insert Sequence:

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTCT GATCGATGGG ATTGACTCAG GGAGTGATTA CATCATCAAG 
GAGAAGACAG TCTTACTGCA GAAGAAGGAC AGTGAGGGGT TCGGGTTCGT GCTCCGGGGG 
GCCAAGGCGC AGACCCCCAT CGAGGAATTC ACCCCCACCC CGGCCTTCCC GGCGCTGCAG 
TACCTCGAGT CTGTGGACGA GGGTGGCGTG GCATGGCGGG CTGGACTACG GATGGGAGAC 
TTCCTCATCG AGGTGAATGG ACAGAATGTA GTGAAGGTTG GCCACCGCCA GGTGGTCAAC 
ATGATCCGAC AGGGAGGCAA CACACTGATG GTCAAGGTGG TGATGGTGAC CAGACACCCA 
GACATGGACG AGGCTGTCCA CAAGAAAGCA TCCCAGCAGG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCC TGCGTTCCAA GTCTATGACC TCAGAGCTGG AGGAAATGGT CTCCCCCTGG 
AAGAAGAAAA TTGAATACGA GCAACAGCCC GCGGCAGTGC CCAGCATGGA GAAAAAGAGG 
ACTGTGTATC AGATGGCACT CAACAAACTG GATGAGATCT TGGCCGCTGC TCAGCAGACC 
ATCAGCGCAA GTGAGAGTCC TGGGCCTGGT GGCCTGGCAT CCCTGGGCAA GCACCGACCC 
AAAGGATTCT TTGCCACTGA GTCGAGCTTT GATCCTCACC ACCGCTCCCA GCCAAGTTAT 
GACCGGCCTT CTTTCCTGCC TCCTGGACCC GGCCTTATGC TCCGGCAGAA ATCTATCGGG 
GCTGCGGAAG ACGACAGACC CTACCTAGCA CCCCCAGCCA TGAAATTCAG TCGTAGCCTG 
TCAGTCCCCG GTTCAGAGGA CATACCCCCA CCACCCACTA CATCACCACC AGAGCCGCCC 
TATAGCACAC CTCCGGCCCC CTCCTCCTCC GGCCGCCTCA CTCCCTCGCC TCGGGGAGGG 
CCCTTTAACC CCAGTTCTGG GGGTCCCCTT CCCGCCTCCT CCCCTTCATC TTTCGATGGG 
CCATCTCCTC CTGACACCCG TGGTGGGGGC CGAGAAAAGA GCCTATATCA CAGCGCAGCC 
CTGCCCCCTG CCCACCACCA TCCACCTCAT CACCACCATC ATCACGCGCC ACCTCCCCAG 
CCCCACCATC ACCATGCACA TCCTCCTCAC CCACCGGAGA TGGAGACAGG AGGTTCCCCC 
GACGATCCCC CACCTCGATT GGCCCTGGGA CCCCAGCCCA GCCTTCGAGG CTGGCGAGGT 
GGAGGACCCA GCCCGACCTC GGGGGCCCCA TCACCTTCCC ACCACAGTAG CAGCGGAGGC 
AGCAGCGGCC CTACTCAGGC TCCCGCCCTG CGCTACTTCC AACTGCCCCC GAGGGCGGCC 
AGTGCGGCTA TGTATGTACC AGCACGTTCC GGCCGCGGTC GCAAGGGCCC ACTGGTCAAG 
CAGACCAAAG TGGAAGGCGA GCCCCAAAAG GGCAGCATCC CTTCCGCGTC CTCTCCAACA 
TCCCCGGCGT TGCCTCGGTC TGAACCACCT CCGGCCGGGC CGTCGGAGAA AAATTCCATC 
CCTATCCCGA CCATCATCAT CAAAGCCCCA TCTACCAGTA GCAGCGGCCG CAGCAGTCAG 
GGAAGCAGCA CAGAGGCTGA GCCCCCCACC CAGCCCGATG GCGCGGGCGG CGGCGGATCC 
TCGCCTAGCC CTGCACCTGC CACGTCCCCT GTGCCGCCAT CCCCGTCGCC CGTGCCCACT 
CCCGCGTCAC CCAGCGGCCC GGCCACCCTG GATTTCACTA GCCAGTTTGG AGCAGCCCTA 
GTAGGCGCGG CGCGGAGGGA GGGAGGCTGG CAGAACGAAG CCCGGCGGCG GTCCACGCTG 
TTCCTGTCCA CCGACGCAGG AGATGAGGAT GGAGGCGACA GCGGGCTGGG CCCGGGGGGG 
CCCCCGGGTC CCCGGCTGCG CCATTCCAAA TCCATTGACG AAGGCATGTT CTCCGCAGAG 
CCGTACCTCC GGCTGGAGTC AGGGGGCAGC AGTGGGGGCT ACGGGGCCTA CGCAGCGGGC 
AGCCGAGCCT ACGGGGGCAG CGGGAGCAGC AGCGCCTTCA CCAGCTTCCT CCCCCCGCGG 
CCCCTGGTGC ATCCGCTGAC CGGCAAAGCC TTGGATCCCG CCTCTCCGCT GGGGTTGGCC 
TTGGCCGCCC GGGAGCGGGC CTTGAAGGAG TCCTCGGAGG GTGGGGGCAC CCCTCAGCCG 
CCCCCGAGAC CTCCATCACC ACGCTACGAC GCCCCACCGC CTACCCTTCA CCACCACTCG 
CCCCATTCAC CCCATTCGCC ACATGCGCGT CACGAGCCAG TGTTGCGCCT CTGGGGGGAC 
CCCGCGCGCC GGGAGCTGGG GTACCGGGCA GGCCTAGGGA GTCAGGAGAA GGCCCTTACC 
GCCAGTCCAC CTGCAGCTCG CCGCTCCCTA CTGCACCGTC TGCCCCCGAC GGCTCCCGGG 
GTCGGGCCGC TCCTGCTGCA GCTGGGGCCG GAGCCCCCAA CTCCCCATCC TGGGGTGAGC 
AAGGCCTGGC GCACAGCAGC CCCGGAGGAG CCGGAGCGGC TGCCGCTGCA CGTGCGATTC 
CTTGAGAATT GCCAAGCCCG GCCTCCCCCA GCTGGCACGA GAGGGTCCTC TACTGAAGAT 
GGGCCTGGGG TCCCGCCTCC CAGCCCTCGC AGAGTCTTGC CAACCTCTCC CACCTCCCCA 
CGAGGCAACG AGGAGAACGG CCTTCCGCTA CTGGTCCTGC CACCGCCAGC CCCTTCGGTG 
GATGTGGACG ACGGCGAGTT CTTGTTTGCG GAGCCGCTGC CTCCACCCCT GGAGTTTTCC 
AATAGTTTCG AAAAGCCAGA GTCGCCTCTC ACACCCGGGC CTCCTCACCC ACTACCTGAC 
CCTCCCTCCC CGGCTACCCC TCTACCCGCA GCCCCACCTC CCGCTGTAGC CGCAGCCCCT 
CCCACCTTGG ACTCCACAGC ATCCAGCCTG ACCTCTTACG ACAGCGAAGT GGCCACACTG 
ACCCAGGGGG CTCCCGCGGC TCCTGGGGAT CCTCCAGCTC CAGGCCCGCC GGCCCCGGCA 
GCCCCAGCTC CCCCAGCACC GCAGCCAGGC CCTGATCCTC CACCTGGCAC AGATTCTGGA 
ATTGAGGAGG TGGACAGTCG GAGTAGCAGT GATCACCCGC TGGAGACCAT CAGCAGCGCA 
TCCACACTCA GCAGCTTGTC CGCCGAAGGA GGAGGCAATA CAGGGGGCGT GGCTGGGGGC 
GGGGCCGGGG TGGCCAGTGG GACGGAGCTC CTGGACACCT ACGTTGCCTA CCTGGATGGC 
CAGGCCTTCG GGGGCAGTGG AACTCCTGGC CCACCATATC CTCCCCAGCT CATGACTCCT 
TCTAAGCTCC GGGGCAGGGC GCTTGGGACT AGTGGAAATT TAAGACCTGG TCCCAGTGGG 
GGACTCCGAG ATCCTGTCAC CCCCACCAGC CCCACTGTCT CTGTGACCGG AGCTGGAACA 
GATGGGCTAC TGGCCTTGAG TGCTTGCCCT GGACCCTCAA CGGCAGGTGT GGCAGGGGGT 
CCCGTGGCAG TTGAGCCAGA AGTCCCGCCT GTACCTTTGC CCGCTGCCTC ATCCCTGCCT 
CGGAAGCTAT TACCGTGGGA GGAGGGTCCG GGACCACCAC CACCACCCCT GCCAGGGCCC 
CTGTCCCAGC CTCAGGCCTC TGCCTTGGCC ACGGTCAAAG CCAGCATCAT CAGCGAACTC 
AGCTCCAAGC TCCAGCAGTT TGGTGGGTCC TCTACCGCAG GAGGAGCTCT GCCTTGGGCT 
CGGGGAGGCA GCGGGGGCAG CACAGACAGC CACCACGGAG GAGCCAGTTA CATCCCAGAG 
AGGACGTCCT CCCTGCAGCG GCAGAGGAAA CGGACCCCTA AGAAGCACAC TTTCAGCTCC 
ATGGTCACCA TAACCCATGG CCCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shank1 ( XM_017589761.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445250.1 Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X5, mRNA.

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CloneID	ORa22786
Clone ID Related Accession (Same CDS sequence)	XM_017589761.1
Accession Version	XM_017589761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTCT GATCGATGGG ATTGACTCAG GGAGTGATTA CATCATCAAG 
GAGAAGACAG TCTTACTGCA GAAGAAGGAC AGTGAGGGGT TCGGGTTCGT GCTCCGGGGG 
GCCAAGGCGC AGACCCCCAT CGAGGAATTC ACCCCCACCC CGGCCTTCCC GGCGCTGCAG 
TACCTCGAGT CTGTGGACGA GGGTGGCGTG GCATGGCGGG CTGGACTACG GATGGGAGAC 
TTCCTCATCG AGGTGAATGG ACAGAATGTA GTGAAGGTTG GCCACCGCCA GGTGGTCAAC 
ATGATCCGAC AGGGAGGCAA CACACTGATG GTCAAGGTGG TGATGGTGAC CAGACACCCA 
GACATGGACG AGGCTGTCCA CAAGAAAGCA TCCCAGCAGG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCC TGCGTTCCAA GTCTATGACC TCAGAGCTGG AGGAAATGGT CTCCCCCTGG 
AAGAAGAAAA TTGAATACGA GCAACAGCCC GCGGCAGTGC CCAGCATGGA GAAAAAGAGG 
ACTGTGTATC AGATGGCACT CAACAAACTG GATGAGATCT TGGCCGCTGC TCAGCAGACC 
ATCAGCGCAA GTGAGAGTCC TGGGCCTGGT GGCCTGGCAT CCCTGGGCAA GCACCGACCC 
AAAGGATTCT TTGCCACTGA GTCGAGCTTT GATCCTCACC ACCGCTCCCA GCCAAGTTAT 
GACCGGCCTT CTTTCCTGCC TCCTGGACCC GGCCTTATGC TCCGGCAGAA ATCTATCGGG 
GCTGCGGAAG ACGACAGACC CTACCTAGCA CCCCCAGCCA TGAAATTCAG TCGTAGCCTG 
TCAGTCCCCG GTTCAGAGGA CATACCCCCA CCACCCACTA CATCACCACC AGAGCCGCCC 
TATAGCACAC CTCCGGCCCC CTCCTCCTCC GGCCGCCTCA CTCCCTCGCC TCGGGGAGGG 
CCCTTTAACC CCAGTTCTGG GGGTCCCCTT CCCGCCTCCT CCCCTTCATC TTTCGATGGG 
CCATCTCCTC CTGACACCCG TGGTGGGGGC CGAGAAAAGA GCCTATATCA CAGCGCAGCC 
CTGCCCCCTG CCCACCACCA TCCACCTCAT CACCACCATC ATCACGCGCC ACCTCCCCAG 
CCCCACCATC ACCATGCACA TCCTCCTCAC CCACCGGAGA TGGAGACAGG AGGTTCCCCC 
GACGATCCCC CACCTCGATT GGCCCTGGGA CCCCAGCCCA GCCTTCGAGG CTGGCGAGGT 
GGAGGACCCA GCCCGACCTC GGGGGCCCCA TCACCTTCCC ACCACAGTAG CAGCGGAGGC 
AGCAGCGGCC CTACTCAGGC TCCCGCCCTG CGCTACTTCC AACTGCCCCC GAGGGCGGCC 
AGTGCGGCTA TGTATGTACC AGCACGTTCC GGCCGCGGTC GCAAGGGCCC ACTGGTCAAG 
CAGACCAAAG TGGAAGGCGA GCCCCAAAAG GGCAGCATCC CTTCCGCGTC CTCTCCAACA 
TCCCCGGCGT TGCCTCGGTC TGAACCACCT CCGGCCGGGC CGTCGGAGAA AAATTCCATC 
CCTATCCCGA CCATCATCAT CAAAGCCCCA TCTACCAGTA GCAGCGGCCG CAGCAGTCAG 
GGAAGCAGCA CAGAGGCTGA GCCCCCCACC CAGCCCGATG GCGCGGGCGG CGGCGGATCC 
TCGCCTAGCC CTGCACCTGC CACGTCCCCT GTGCCGCCAT CCCCGTCGCC CGTGCCCACT 
CCCGCGTCAC CCAGCGGCCC GGCCACCCTG GATTTCACTA GCCAGTTTGG AGCAGCCCTA 
GTAGGCGCGG CGCGGAGGGA GGGAGGCTGG CAGAACGAAG CCCGGCGGCG GTCCACGCTG 
TTCCTGTCCA CCGACGCAGG AGATGAGGAT GGAGGCGACA GCGGGCTGGG CCCGGGGGGG 
CCCCCGGGTC CCCGGCTGCG CCATTCCAAA TCCATTGACG AAGGCATGTT CTCCGCAGAG 
CCGTACCTCC GGCTGGAGTC AGGGGGCAGC AGTGGGGGCT ACGGGGCCTA CGCAGCGGGC 
AGCCGAGCCT ACGGGGGCAG CGGGAGCAGC AGCGCCTTCA CCAGCTTCCT CCCCCCGCGG 
CCCCTGGTGC ATCCGCTGAC CGGCAAAGCC TTGGATCCCG CCTCTCCGCT GGGGTTGGCC 
TTGGCCGCCC GGGAGCGGGC CTTGAAGGAG TCCTCGGAGG GTGGGGGCAC CCCTCAGCCG 
CCCCCGAGAC CTCCATCACC ACGCTACGAC GCCCCACCGC CTACCCTTCA CCACCACTCG 
CCCCATTCAC CCCATTCGCC ACATGCGCGT CACGAGCCAG TGTTGCGCCT CTGGGGGGAC 
CCCGCGCGCC GGGAGCTGGG GTACCGGGCA GGCCTAGGGA GTCAGGAGAA GGCCCTTACC 
GCCAGTCCAC CTGCAGCTCG CCGCTCCCTA CTGCACCGTC TGCCCCCGAC GGCTCCCGGG 
GTCGGGCCGC TCCTGCTGCA GCTGGGGCCG GAGCCCCCAA CTCCCCATCC TGGGGTGAGC 
AAGGCCTGGC GCACAGCAGC CCCGGAGGAG CCGGAGCGGC TGCCGCTGCA CGTGCGATTC 
CTTGAGAATT GCCAAGCCCG GCCTCCCCCA GCTGGCACGA GAGGGTCCTC TACTGAAGAT 
GGGCCTGGGG TCCCGCCTCC CAGCCCTCGC AGAGTCTTGC CAACCTCTCC CACCTCCCCA 
CGAGGCAACG AGGAGAACGG CCTTCCGCTA CTGGTCCTGC CACCGCCAGC CCCTTCGGTG 
GATGTGGACG ACGGCGAGTT CTTGTTTGCG GAGCCGCTGC CTCCACCCCT GGAGTTTTCC 
AATAGTTTCG AAAAGCCAGA GTCGCCTCTC ACACCCGGGC CTCCTCACCC ACTACCTGAC 
CCTCCCTCCC CGGCTACCCC TCTACCCGCA GCCCCACCTC CCGCTGTAGC CGCAGCCCCT 
CCCACCTTGG ACTCCACAGC ATCCAGCCTG ACCTCTTACG ACAGCGAAGT GGCCACACTG 
ACCCAGGGGG CTCCCGCGGC TCCTGGGGAT CCTCCAGCTC CAGGCCCGCC GGCCCCGGCA 
GCCCCAGCTC CCCCAGCACC GCAGCCAGGC CCTGATCCTC CACCTGGCAC AGATTCTGGA 
ATTGAGGAGG TGGACAGTCG GAGTAGCAGT GATCACCCGC TGGAGACCAT CAGCAGCGCA 
TCCACACTCA GCAGCTTGTC CGCCGAAGGA GGAGGCAATA CAGGGGGCGT GGCTGGGGGC 
GGGGCCGGGG TGGCCAGTGG GACGGAGCTC CTGGACACCT ACGTTGCCTA CCTGGATGGC 
CAGGCCTTCG GGGGCAGTGG AACTCCTGGC CCACCATATC CTCCCCAGCT CATGACTCCT 
TCTAAGCTCC GGGGCAGGGC GCTTGGGACT AGTGGAAATT TAAGACCTGG TCCCAGTGGG 
GGACTCCGAG ATCCTGTCAC CCCCACCAGC CCCACTGTCT CTGTGACCGG AGCTGGAACA 
GATGGGCTAC TGGCCTTGAG TGCTTGCCCT GGACCCTCAA CGGCAGGTGT GGCAGGGGGT 
CCCGTGGCAG TTGAGCCAGA AGTCCCGCCT GTACCTTTGC CCGCTGCCTC ATCCCTGCCT 
CGGAAGCTAT TACCGTGGGA GGAGGGTCCG GGACCACCAC CACCACCCCT GCCAGGGCCC 
CTGTCCCAGC CTCAGGCCTC TGCCTTGGCC ACGGTCAAAG CCAGCATCAT CAGCGAACTC 
AGCTCCAAGC TCCAGCAGTT TGGTGGGTCC TCTACCGCAG GAGGAGCTCT GCCTTGGGCT 
CGGGGAGGCA GCGGGGGCAG CACAGACAGC CACCACGGAG GAGCCAGTTA CATCCCAGAG 
AGGACGTCCT CCCTGCAGCG GCAGAGGCAG TATAGAATAG TGGTTAAGAG CTCAGACTTT 
GGTGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445250.1
CDS	102..5933
Translation	MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPQGTRGRGSGAPGNLASTRGLQGRSMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKETCARILLYRGANKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARRRGPPGAGLTVPPALLRANSDTSMALPDWMVFSAPGASSSGTPGPTSGPQGQSQPSAPSTKLSSGTLRSASSPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPRDGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQAQGEGEISLSKGEKIKVLSIGEGGFWEGQVKGRVGWFPSDCLEEVANRSQEGRQESRSDKAKRLFRHYTVGSYDSFDAPSLIDGIDSGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKASQQAKRLPPPAISLRSKSMTSELEEMVSPWKKKIEYEQQPAAVPSMEKKRTVYQMALNKLDEILAAAQQTISASESPGPGGLASLGKHRPKGFFATESSFDPHHRSQPSYDRPSFLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPSSGGPLPASSPSSFDGPSPPDTRGGGREKSLYHSAALPPAHHHPPHHHHHHAPPPQPHHHHAHPPHPPEMETGGSPDDPPPRLALGPQPSLRGWRGGGPSPTSGAPSPSHHSSSGGSSGPTQAPALRYFQLPPRAASAAMYVPARSGRGRKGPLVKQTKVEGEPQKGSIPSASSPTSPALPRSEPPPAGPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQPDGAGGGGSSPSPAPATSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDAGDEDGGDSGLGPGGPPGPRLRHSKSIDEGMFSAEPYLRLESGGSSGGYGAYAAGSRAYGGSGSSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGTPQPPPRPPSPRYDAPPPTLHHHSPHSPHSPHARHEPVLRLWGDPARRELGYRAGLGSQEKALTASPPAARRSLLHRLPPTAPGVGPLLLQLGPEPPTPHPGVSKAWRTAAPEEPERLPLHVRFLENCQARPPPAGTRGSSTEDGPGVPPPSPRRVLPTSPTSPRGNEENGLPLLVLPPPAPSVDVDDGEFLFAEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPSPATPLPAAPPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPAPGPPAPAAPAPPAPQPGPDPPPGTDSGIEEVDSRSSSDHPLETISSASTLSSLSAEGGGNTGGVAGGGAGVASGTELLDTYVAYLDGQAFGGSGTPGPPYPPQLMTPSKLRGRALGTSGNLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLALSACPGPSTAGVAGGPVAVEPEVPPVPLPAASSLPRKLLPWEEGPGPPPPPLPGPLSQPQASALATVKASIISELSSKLQQFGGSSTAGGALPWARGGSGGSTDSHHGGASYIPERTSSLQRQRQYRIVVKSSDFGDF

Target ORF information:

RefSeq Version	XM_017589761.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589761.1

ORF Insert Sequence:

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTCT GATCGATGGG ATTGACTCAG GGAGTGATTA CATCATCAAG 
GAGAAGACAG TCTTACTGCA GAAGAAGGAC AGTGAGGGGT TCGGGTTCGT GCTCCGGGGG 
GCCAAGGCGC AGACCCCCAT CGAGGAATTC ACCCCCACCC CGGCCTTCCC GGCGCTGCAG 
TACCTCGAGT CTGTGGACGA GGGTGGCGTG GCATGGCGGG CTGGACTACG GATGGGAGAC 
TTCCTCATCG AGGTGAATGG ACAGAATGTA GTGAAGGTTG GCCACCGCCA GGTGGTCAAC 
ATGATCCGAC AGGGAGGCAA CACACTGATG GTCAAGGTGG TGATGGTGAC CAGACACCCA 
GACATGGACG AGGCTGTCCA CAAGAAAGCA TCCCAGCAGG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCC TGCGTTCCAA GTCTATGACC TCAGAGCTGG AGGAAATGGT CTCCCCCTGG 
AAGAAGAAAA TTGAATACGA GCAACAGCCC GCGGCAGTGC CCAGCATGGA GAAAAAGAGG 
ACTGTGTATC AGATGGCACT CAACAAACTG GATGAGATCT TGGCCGCTGC TCAGCAGACC 
ATCAGCGCAA GTGAGAGTCC TGGGCCTGGT GGCCTGGCAT CCCTGGGCAA GCACCGACCC 
AAAGGATTCT TTGCCACTGA GTCGAGCTTT GATCCTCACC ACCGCTCCCA GCCAAGTTAT 
GACCGGCCTT CTTTCCTGCC TCCTGGACCC GGCCTTATGC TCCGGCAGAA ATCTATCGGG 
GCTGCGGAAG ACGACAGACC CTACCTAGCA CCCCCAGCCA TGAAATTCAG TCGTAGCCTG 
TCAGTCCCCG GTTCAGAGGA CATACCCCCA CCACCCACTA CATCACCACC AGAGCCGCCC 
TATAGCACAC CTCCGGCCCC CTCCTCCTCC GGCCGCCTCA CTCCCTCGCC TCGGGGAGGG 
CCCTTTAACC CCAGTTCTGG GGGTCCCCTT CCCGCCTCCT CCCCTTCATC TTTCGATGGG 
CCATCTCCTC CTGACACCCG TGGTGGGGGC CGAGAAAAGA GCCTATATCA CAGCGCAGCC 
CTGCCCCCTG CCCACCACCA TCCACCTCAT CACCACCATC ATCACGCGCC ACCTCCCCAG 
CCCCACCATC ACCATGCACA TCCTCCTCAC CCACCGGAGA TGGAGACAGG AGGTTCCCCC 
GACGATCCCC CACCTCGATT GGCCCTGGGA CCCCAGCCCA GCCTTCGAGG CTGGCGAGGT 
GGAGGACCCA GCCCGACCTC GGGGGCCCCA TCACCTTCCC ACCACAGTAG CAGCGGAGGC 
AGCAGCGGCC CTACTCAGGC TCCCGCCCTG CGCTACTTCC AACTGCCCCC GAGGGCGGCC 
AGTGCGGCTA TGTATGTACC AGCACGTTCC GGCCGCGGTC GCAAGGGCCC ACTGGTCAAG 
CAGACCAAAG TGGAAGGCGA GCCCCAAAAG GGCAGCATCC CTTCCGCGTC CTCTCCAACA 
TCCCCGGCGT TGCCTCGGTC TGAACCACCT CCGGCCGGGC CGTCGGAGAA AAATTCCATC 
CCTATCCCGA CCATCATCAT CAAAGCCCCA TCTACCAGTA GCAGCGGCCG CAGCAGTCAG 
GGAAGCAGCA CAGAGGCTGA GCCCCCCACC CAGCCCGATG GCGCGGGCGG CGGCGGATCC 
TCGCCTAGCC CTGCACCTGC CACGTCCCCT GTGCCGCCAT CCCCGTCGCC CGTGCCCACT 
CCCGCGTCAC CCAGCGGCCC GGCCACCCTG GATTTCACTA GCCAGTTTGG AGCAGCCCTA 
GTAGGCGCGG CGCGGAGGGA GGGAGGCTGG CAGAACGAAG CCCGGCGGCG GTCCACGCTG 
TTCCTGTCCA CCGACGCAGG AGATGAGGAT GGAGGCGACA GCGGGCTGGG CCCGGGGGGG 
CCCCCGGGTC CCCGGCTGCG CCATTCCAAA TCCATTGACG AAGGCATGTT CTCCGCAGAG 
CCGTACCTCC GGCTGGAGTC AGGGGGCAGC AGTGGGGGCT ACGGGGCCTA CGCAGCGGGC 
AGCCGAGCCT ACGGGGGCAG CGGGAGCAGC AGCGCCTTCA CCAGCTTCCT CCCCCCGCGG 
CCCCTGGTGC ATCCGCTGAC CGGCAAAGCC TTGGATCCCG CCTCTCCGCT GGGGTTGGCC 
TTGGCCGCCC GGGAGCGGGC CTTGAAGGAG TCCTCGGAGG GTGGGGGCAC CCCTCAGCCG 
CCCCCGAGAC CTCCATCACC ACGCTACGAC GCCCCACCGC CTACCCTTCA CCACCACTCG 
CCCCATTCAC CCCATTCGCC ACATGCGCGT CACGAGCCAG TGTTGCGCCT CTGGGGGGAC 
CCCGCGCGCC GGGAGCTGGG GTACCGGGCA GGCCTAGGGA GTCAGGAGAA GGCCCTTACC 
GCCAGTCCAC CTGCAGCTCG CCGCTCCCTA CTGCACCGTC TGCCCCCGAC GGCTCCCGGG 
GTCGGGCCGC TCCTGCTGCA GCTGGGGCCG GAGCCCCCAA CTCCCCATCC TGGGGTGAGC 
AAGGCCTGGC GCACAGCAGC CCCGGAGGAG CCGGAGCGGC TGCCGCTGCA CGTGCGATTC 
CTTGAGAATT GCCAAGCCCG GCCTCCCCCA GCTGGCACGA GAGGGTCCTC TACTGAAGAT 
GGGCCTGGGG TCCCGCCTCC CAGCCCTCGC AGAGTCTTGC CAACCTCTCC CACCTCCCCA 
CGAGGCAACG AGGAGAACGG CCTTCCGCTA CTGGTCCTGC CACCGCCAGC CCCTTCGGTG 
GATGTGGACG ACGGCGAGTT CTTGTTTGCG GAGCCGCTGC CTCCACCCCT GGAGTTTTCC 
AATAGTTTCG AAAAGCCAGA GTCGCCTCTC ACACCCGGGC CTCCTCACCC ACTACCTGAC 
CCTCCCTCCC CGGCTACCCC TCTACCCGCA GCCCCACCTC CCGCTGTAGC CGCAGCCCCT 
CCCACCTTGG ACTCCACAGC ATCCAGCCTG ACCTCTTACG ACAGCGAAGT GGCCACACTG 
ACCCAGGGGG CTCCCGCGGC TCCTGGGGAT CCTCCAGCTC CAGGCCCGCC GGCCCCGGCA 
GCCCCAGCTC CCCCAGCACC GCAGCCAGGC CCTGATCCTC CACCTGGCAC AGATTCTGGA 
ATTGAGGAGG TGGACAGTCG GAGTAGCAGT GATCACCCGC TGGAGACCAT CAGCAGCGCA 
TCCACACTCA GCAGCTTGTC CGCCGAAGGA GGAGGCAATA CAGGGGGCGT GGCTGGGGGC 
GGGGCCGGGG TGGCCAGTGG GACGGAGCTC CTGGACACCT ACGTTGCCTA CCTGGATGGC 
CAGGCCTTCG GGGGCAGTGG AACTCCTGGC CCACCATATC CTCCCCAGCT CATGACTCCT 
TCTAAGCTCC GGGGCAGGGC GCTTGGGACT AGTGGAAATT TAAGACCTGG TCCCAGTGGG 
GGACTCCGAG ATCCTGTCAC CCCCACCAGC CCCACTGTCT CTGTGACCGG AGCTGGAACA 
GATGGGCTAC TGGCCTTGAG TGCTTGCCCT GGACCCTCAA CGGCAGGTGT GGCAGGGGGT 
CCCGTGGCAG TTGAGCCAGA AGTCCCGCCT GTACCTTTGC CCGCTGCCTC ATCCCTGCCT 
CGGAAGCTAT TACCGTGGGA GGAGGGTCCG GGACCACCAC CACCACCCCT GCCAGGGCCC 
CTGTCCCAGC CTCAGGCCTC TGCCTTGGCC ACGGTCAAAG CCAGCATCAT CAGCGAACTC 
AGCTCCAAGC TCCAGCAGTT TGGTGGGTCC TCTACCGCAG GAGGAGCTCT GCCTTGGGCT 
CGGGGAGGCA GCGGGGGCAG CACAGACAGC CACCACGGAG GAGCCAGTTA CATCCCAGAG 
AGGACGTCCT CCCTGCAGCG GCAGAGGCAG TATAGAATAG TGGTTAAGAG CTCAGACTTT 
GGTGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shank1 ( XM_017589758.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445247.1 Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X1, mRNA.

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CloneID	ORa56860
Clone ID Related Accession (Same CDS sequence)	XM_017589758.1
Accession Version	XM_017589758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTCT GATCGATGGG ATTGACTCAG GGAGTGATTA CATCATCAAG 
GAGAAGACAG TCTTACTGCA GAAGAAGGAC AGTGAGGGGT TCGGGTTCGT GCTCCGGGGG 
GCCAAGGCGC AGACCCCCAT CGAGGAATTC ACCCCCACCC CGGCCTTCCC GGCGCTGCAG 
TACCTCGAGT CTGTGGACGA GGGTGGCGTG GCATGGCGGG CTGGACTACG GATGGGAGAC 
TTCCTCATCG AGGTGAATGG ACAGAATGTA GTGAAGGTTG GCCACCGCCA GGTGGTCAAC 
ATGATCCGAC AGGGAGGCAA CACACTGATG GTCAAGGTGG TGATGGTGAC CAGACACCCA 
GACATGGACG AGGCTGTCCA CAAGAAAGCA TCCCAGCAGG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCC TGCGTTCCAA GTCTATGACC TCAGAGCTGG AGGAAATGGT CTCCCCCTGG 
AAGAAGAAAA TTGAATACGA GCAACAGCCC GCGGCAGTGC CCAGCATGGA GAAAAAGAGG 
ACTGTGTATC AGATGGCACT CAACAAACTG GATGAGATCT TGGCCGCTGC TCAGCAGACC 
ATCAGCGCAA GTGAGAGTCC TGGGCCTGGT GGCCTGGCAT CCCTGGGCAA GCACCGACCC 
AAAGGATTCT TTGCCACTGA GTCGAGCTTT GATCCTCACC ACCGCTCCCA GCCAAGTTAT 
GACCGGCCTT CTTTCCTGCC TCCTGGACCC GGCCTTATGC TCCGGCAGAA ATCTATCGGG 
GCTGCGGAAG ACGACAGACC CTACCTAGCA CCCCCAGCCA TGAAATTCAG TCGTAGCCTG 
TCAGTCCCCG GTTCAGAGGA CATACCCCCA CCACCCACTA CATCACCACC AGAGCCGCCC 
TATAGCACAC CTCCGGCCCC CTCCTCCTCC GGCCGCCTCA CTCCCTCGCC TCGGGGAGGG 
CCCTTTAACC CCAGTTCTGG GGGTCCCCTT CCCGCCTCCT CCCCTTCATC TTTCGATGGG 
CCATCTCCTC CTGACACCCG TGGTGGGGGC CGAGAAAAGA GCCTATATCA CAGCGCAGCC 
CTGCCCCCTG CCCACCACCA TCCACCTCAT CACCACCATC ATCACGCGCC ACCTCCCCAG 
CCCCACCATC ACCATGCACA TCCTCCTCAC CCACCGGAGA TGGAGACAGG AGGTTCCCCC 
GACGATCCCC CACCTCGATT GGCCCTGGGA CCCCAGCCCA GCCTTCGAGG CTGGCGAGGT 
GGAGGACCCA GCCCGACCTC GGGGGCCCCA TCACCTTCCC ACCACAGTAG CAGCGGAGGC 
AGCAGCGGCC CTACTCAGGC TCCCGCCCTG CGCTACTTCC AACTGCCCCC GAGGGCGGCC 
AGTGCGGCTA TGTATGTACC AGCACGTTCC GGCCGCGGTC GCAAGGGCCC ACTGGTCAAG 
CAGACCAAAG TGGAAGGCGA GCCCCAAAAG GGCAGCATCC CTTCCGCGTC CTCTCCAACA 
TCCCCGGCGT TGCCTCGGTC TGAACCACCT CCGGCCGGGC CGTCGGAGAA AAATTCCATC 
CCTATCCCGA CCATCATCAT CAAAGCCCCA TCTACCAGTA GCAGCGGCCG CAGCAGTCAG 
GGAAGCAGCA CAGAGGCTGA GCCCCCCACC CAGCCCGATG GCGCGGGCGG CGGCGGATCC 
TCGCCTAGCC CTGCACCTGC CACGTCCCCT GTGCCGCCAT CCCCGTCGCC CGTGCCCACT 
CCCGCGTCAC CCAGCGGCCC GGCCACCCTG GATTTCACTA GCCAGTTTGG AGCAGCCCTA 
GTAGGCGCGG CGCGGAGGGA GGGAGGCTGG CAGAACGAAG CCCGGCGGCG GTCCACGCTG 
TTCCTGTCCA CCGACGCAGG AGATGAGGAT GGAGGCGACA GCGGGCTGGG CCCGGGGGGG 
CCCCCGGGTC CCCGGCTGCG CCATTCCAAA TCCATTGACG AAGGCATGTT CTCCGCAGAG 
CCGTACCTCC GGCTGGAGTC AGGGGGCAGC AGTGGGGGCT ACGGGGCCTA CGCAGCGGGC 
AGCCGAGCCT ACGGGGGCAG CGGGAGCAGC AGCGCCTTCA CCAGCTTCCT CCCCCCGCGG 
CCCCTGGTGC ATCCGCTGAC CGGCAAAGCC TTGGATCCCG CCTCTCCGCT GGGGTTGGCC 
TTGGCCGCCC GGGAGCGGGC CTTGAAGGAG TCCTCGGAGG GTGGGGGCAC CCCTCAGCCG 
CCCCCGAGAC CTCCATCACC ACGCTACGAC GCCCCACCGC CTACCCTTCA CCACCACTCG 
CCCCATTCAC CCCATTCGCC ACATGCGCGT CACGAGCCAG TGTTGCGCCT CTGGGGGGAC 
CCCGCGCGCC GGGAGCTGGG GTACCGGGCA GGCCTAGGGA GTCAGGAGAA GGCCCTTACC 
GCCAGTCCAC CTGCAGCTCG CCGCTCCCTA CTGCACCGTC TGCCCCCGAC GGCTCCCGGG 
GTCGGGCCGC TCCTGCTGCA GCTGGGGCCG GAGCCCCCAA CTCCCCATCC TGGGGTGAGC 
AAGGCCTGGC GCACAGCAGC CCCGGAGGAG CCGGAGCGGC TGCCGCTGCA CGTGCGATTC 
CTTGAGAATT GCCAAGCCCG GCCTCCCCCA GCTGGCACGA GAGGGTCCTC TACTGAAGAT 
GGGCCTGGGG TCCCGCCTCC CAGCCCTCGC AGAGTCTTGC CAACCTCTCC CACCTCCCCA 
CGAGGCAACG AGGAGAACGG CCTTCCGCTA CTGGTCCTGC CACCGCCAGC CCCTTCGGTG 
GATGTGGACG ACGGCGAGTT CTTGTTTGCG GAGCCGCTGC CTCCACCCCT GGAGTTTTCC 
AATAGTTTCG AAAAGCCAGA GTCGCCTCTC ACACCCGGGC CTCCTCACCC ACTACCTGAC 
CCTCCCTCCC CGGCTACCCC TCTACCCGCA GCCCCACCTC CCGCTGTAGC CGCAGCCCCT 
CCCACCTTGG ACTCCACAGC ATCCAGCCTG ACCTCTTACG ACAGCGAAGT GGCCACACTG 
ACCCAGGGGG CTCCCGCGGC TCCTGGGGAT CCTCCAGCTC CAGGCCCGCC GGCCCCGGCA 
GCCCCAGCTC CCCCAGCACC GCAGCCAGGC CCTGATCCTC CACCTGGCAC AGATTCTGGA 
ATTGAGGAGG TGGACAGTCG GAGTAGCAGT GATCACCCGC TGGAGACCAT CAGCAGCGCA 
TCCACACTCA GCAGCTTGTC CGCCGAAGGA GGAGGCAATA CAGGGGGCGT GGCTGGGGGC 
GGGGCCGGGG TGGCCAGTGG GACGGAGCTC CTGGACACCT ACGTTGCCTA CCTGGATGGC 
CAGGCCTTCG GGGGCAGTGG AACTCCTGGC CCACCATATC CTCCCCAGCT CATGACTCCT 
TCTAAGCTCC GGGGCAGGGC GCTTGGGACT AGTGGAAATT TAAGACCTGG TCCCAGTGGG 
GGACTCCGAG ATCCTGTCAC CCCCACCAGC CCCACTGTCT CTGTGACCGG AGCTGGAACA 
GATGGGCTAC TGGCCTTGAG TGCTTGCCCT GGACCCTCAA CGGCAGGTGT GGCAGGGGGT 
CCCGTGGCAG TTGAGCCAGA AGTCCCGCCT GTACCTTTGC CCGCTGCCTC ATCCCTGCCT 
CGGAAGCTAT TACCGTGGGA GGAGGGTCCG GGACCACCAC CACCACCCCT GCCAGGGCCC 
CTGTCCCAGC CTCAGGCCTC TGCCTTGGCC ACGGTCAAAG CCAGCATCAT CAGCGAACTC 
AGCTCCAAGC TCCAGCAGTT TGGTGGGTCC TCTACCGCAG GAGGAGCTCT GCCTTGGGCT 
CGGGGAGGCA GCGGGGGCAG CACAGACAGC CACCACGGAG GAGCCAGTTA CATCCCAGAG 
AGGACGTCCT CCCTGCAGCG GCAGAGGCTC TCTGAAGACT CCCAGACCTC TCTCCTCTCC 
AAACCCAGCA GCAGCATCTT TCAGAACTGG CCTAAACCCC CTCTGCCACC ACTCCCCACT 
GGGTCTGGGG TCTCCTCTTC AACAGCTGCA GCCCCGGGAG CTACATCCCC TTCAGCCTCA 
TCTGCCTCAG CTTCCACCAG ACACCTGCAG GGTGTGGAAT TTGAGATGCG GCCTCCGCTG 
CTCCGCCGGG CACCCAGCCC CTCGCTGCTG CCTGCCTCGG ATCACAAGGT CAGCCCAGCG 
CCCAGGCCTT CCTCTTTGCC CATCCTGCCT TCAGGACCCA TCTACCCGGG CCTCTTCGAC 
ATCCGTAGCT CTCCAACCGG AGGGGCGGGA GGCTCGACTG ACCCCTTCGC TCCCGTCTTT 
GTGCCACCAC ACCCCGGGAT ATCTGGGGGT CTTGGAGGAG CCTTGTCTGG GGCCTCTCGC 
TCCCTATCAC CCACCCGCCT GCTCTCGCTG CCCCCGGACA AGCCGTTCGG CGCCAAGCCT 
CTAGGGTTCT GGACCAAGTT TGACGTCGCC GATTGGCTAG AGTGGCTGGG CTTGTCGGAG 
CACCGAGCCC AGTTCCTGGA CCACGAGATT GATGGCTCCC ATCTGCCTGC CTTGACCAAG 
GAGGACTATG TGGACCTGGG TGTAACCAGG GTGGGCCACC GTATGAACAT TGACCGGGCT 
CTCAAATTTT TCCTGGAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445247.1
CDS	206..6709
Translation	MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPQGTRGRGSGAPGNLASTRGLQGRSMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKETCARILLYRGANKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARRRGPPGAGLTVPPALLRANSDTSMALPDWMVFSAPGASSSGTPGPTSGPQGQSQPSAPSTKLSSGTLRSASSPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPRDGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQAQGEGEISLSKGEKIKVLSIGEGGFWEGQVKGRVGWFPSDCLEEVANRSQEGRQESRSDKAKRLFRHYTVGSYDSFDAPSLIDGIDSGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKASQQAKRLPPPAISLRSKSMTSELEEMVSPWKKKIEYEQQPAAVPSMEKKRTVYQMALNKLDEILAAAQQTISASESPGPGGLASLGKHRPKGFFATESSFDPHHRSQPSYDRPSFLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPSSGGPLPASSPSSFDGPSPPDTRGGGREKSLYHSAALPPAHHHPPHHHHHHAPPPQPHHHHAHPPHPPEMETGGSPDDPPPRLALGPQPSLRGWRGGGPSPTSGAPSPSHHSSSGGSSGPTQAPALRYFQLPPRAASAAMYVPARSGRGRKGPLVKQTKVEGEPQKGSIPSASSPTSPALPRSEPPPAGPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQPDGAGGGGSSPSPAPATSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDAGDEDGGDSGLGPGGPPGPRLRHSKSIDEGMFSAEPYLRLESGGSSGGYGAYAAGSRAYGGSGSSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGTPQPPPRPPSPRYDAPPPTLHHHSPHSPHSPHARHEPVLRLWGDPARRELGYRAGLGSQEKALTASPPAARRSLLHRLPPTAPGVGPLLLQLGPEPPTPHPGVSKAWRTAAPEEPERLPLHVRFLENCQARPPPAGTRGSSTEDGPGVPPPSPRRVLPTSPTSPRGNEENGLPLLVLPPPAPSVDVDDGEFLFAEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPSPATPLPAAPPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPAPGPPAPAAPAPPAPQPGPDPPPGTDSGIEEVDSRSSSDHPLETISSASTLSSLSAEGGGNTGGVAGGGAGVASGTELLDTYVAYLDGQAFGGSGTPGPPYPPQLMTPSKLRGRALGTSGNLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLALSACPGPSTAGVAGGPVAVEPEVPPVPLPAASSLPRKLLPWEEGPGPPPPPLPGPLSQPQASALATVKASIISELSSKLQQFGGSSTAGGALPWARGGSGGSTDSHHGGASYIPERTSSLQRQRLSEDSQTSLLSKPSSSIFQNWPKPPLPPLPTGSGVSSSTAAAPGATSPSASSASASTRHLQGVEFEMRPPLLRRAPSPSLLPASDHKVSPAPRPSSLPILPSGPIYPGLFDIRSSPTGGAGGSTDPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKPLGFWTKFDVADWLEWLGLSEHRAQFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLER

Target ORF information:

RefSeq Version	XM_017589758.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589758.1

ORF Insert Sequence:

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTCT GATCGATGGG ATTGACTCAG GGAGTGATTA CATCATCAAG 
GAGAAGACAG TCTTACTGCA GAAGAAGGAC AGTGAGGGGT TCGGGTTCGT GCTCCGGGGG 
GCCAAGGCGC AGACCCCCAT CGAGGAATTC ACCCCCACCC CGGCCTTCCC GGCGCTGCAG 
TACCTCGAGT CTGTGGACGA GGGTGGCGTG GCATGGCGGG CTGGACTACG GATGGGAGAC 
TTCCTCATCG AGGTGAATGG ACAGAATGTA GTGAAGGTTG GCCACCGCCA GGTGGTCAAC 
ATGATCCGAC AGGGAGGCAA CACACTGATG GTCAAGGTGG TGATGGTGAC CAGACACCCA 
GACATGGACG AGGCTGTCCA CAAGAAAGCA TCCCAGCAGG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCC TGCGTTCCAA GTCTATGACC TCAGAGCTGG AGGAAATGGT CTCCCCCTGG 
AAGAAGAAAA TTGAATACGA GCAACAGCCC GCGGCAGTGC CCAGCATGGA GAAAAAGAGG 
ACTGTGTATC AGATGGCACT CAACAAACTG GATGAGATCT TGGCCGCTGC TCAGCAGACC 
ATCAGCGCAA GTGAGAGTCC TGGGCCTGGT GGCCTGGCAT CCCTGGGCAA GCACCGACCC 
AAAGGATTCT TTGCCACTGA GTCGAGCTTT GATCCTCACC ACCGCTCCCA GCCAAGTTAT 
GACCGGCCTT CTTTCCTGCC TCCTGGACCC GGCCTTATGC TCCGGCAGAA ATCTATCGGG 
GCTGCGGAAG ACGACAGACC CTACCTAGCA CCCCCAGCCA TGAAATTCAG TCGTAGCCTG 
TCAGTCCCCG GTTCAGAGGA CATACCCCCA CCACCCACTA CATCACCACC AGAGCCGCCC 
TATAGCACAC CTCCGGCCCC CTCCTCCTCC GGCCGCCTCA CTCCCTCGCC TCGGGGAGGG 
CCCTTTAACC CCAGTTCTGG GGGTCCCCTT CCCGCCTCCT CCCCTTCATC TTTCGATGGG 
CCATCTCCTC CTGACACCCG TGGTGGGGGC CGAGAAAAGA GCCTATATCA CAGCGCAGCC 
CTGCCCCCTG CCCACCACCA TCCACCTCAT CACCACCATC ATCACGCGCC ACCTCCCCAG 
CCCCACCATC ACCATGCACA TCCTCCTCAC CCACCGGAGA TGGAGACAGG AGGTTCCCCC 
GACGATCCCC CACCTCGATT GGCCCTGGGA CCCCAGCCCA GCCTTCGAGG CTGGCGAGGT 
GGAGGACCCA GCCCGACCTC GGGGGCCCCA TCACCTTCCC ACCACAGTAG CAGCGGAGGC 
AGCAGCGGCC CTACTCAGGC TCCCGCCCTG CGCTACTTCC AACTGCCCCC GAGGGCGGCC 
AGTGCGGCTA TGTATGTACC AGCACGTTCC GGCCGCGGTC GCAAGGGCCC ACTGGTCAAG 
CAGACCAAAG TGGAAGGCGA GCCCCAAAAG GGCAGCATCC CTTCCGCGTC CTCTCCAACA 
TCCCCGGCGT TGCCTCGGTC TGAACCACCT CCGGCCGGGC CGTCGGAGAA AAATTCCATC 
CCTATCCCGA CCATCATCAT CAAAGCCCCA TCTACCAGTA GCAGCGGCCG CAGCAGTCAG 
GGAAGCAGCA CAGAGGCTGA GCCCCCCACC CAGCCCGATG GCGCGGGCGG CGGCGGATCC 
TCGCCTAGCC CTGCACCTGC CACGTCCCCT GTGCCGCCAT CCCCGTCGCC CGTGCCCACT 
CCCGCGTCAC CCAGCGGCCC GGCCACCCTG GATTTCACTA GCCAGTTTGG AGCAGCCCTA 
GTAGGCGCGG CGCGGAGGGA GGGAGGCTGG CAGAACGAAG CCCGGCGGCG GTCCACGCTG 
TTCCTGTCCA CCGACGCAGG AGATGAGGAT GGAGGCGACA GCGGGCTGGG CCCGGGGGGG 
CCCCCGGGTC CCCGGCTGCG CCATTCCAAA TCCATTGACG AAGGCATGTT CTCCGCAGAG 
CCGTACCTCC GGCTGGAGTC AGGGGGCAGC AGTGGGGGCT ACGGGGCCTA CGCAGCGGGC 
AGCCGAGCCT ACGGGGGCAG CGGGAGCAGC AGCGCCTTCA CCAGCTTCCT CCCCCCGCGG 
CCCCTGGTGC ATCCGCTGAC CGGCAAAGCC TTGGATCCCG CCTCTCCGCT GGGGTTGGCC 
TTGGCCGCCC GGGAGCGGGC CTTGAAGGAG TCCTCGGAGG GTGGGGGCAC CCCTCAGCCG 
CCCCCGAGAC CTCCATCACC ACGCTACGAC GCCCCACCGC CTACCCTTCA CCACCACTCG 
CCCCATTCAC CCCATTCGCC ACATGCGCGT CACGAGCCAG TGTTGCGCCT CTGGGGGGAC 
CCCGCGCGCC GGGAGCTGGG GTACCGGGCA GGCCTAGGGA GTCAGGAGAA GGCCCTTACC 
GCCAGTCCAC CTGCAGCTCG CCGCTCCCTA CTGCACCGTC TGCCCCCGAC GGCTCCCGGG 
GTCGGGCCGC TCCTGCTGCA GCTGGGGCCG GAGCCCCCAA CTCCCCATCC TGGGGTGAGC 
AAGGCCTGGC GCACAGCAGC CCCGGAGGAG CCGGAGCGGC TGCCGCTGCA CGTGCGATTC 
CTTGAGAATT GCCAAGCCCG GCCTCCCCCA GCTGGCACGA GAGGGTCCTC TACTGAAGAT 
GGGCCTGGGG TCCCGCCTCC CAGCCCTCGC AGAGTCTTGC CAACCTCTCC CACCTCCCCA 
CGAGGCAACG AGGAGAACGG CCTTCCGCTA CTGGTCCTGC CACCGCCAGC CCCTTCGGTG 
GATGTGGACG ACGGCGAGTT CTTGTTTGCG GAGCCGCTGC CTCCACCCCT GGAGTTTTCC 
AATAGTTTCG AAAAGCCAGA GTCGCCTCTC ACACCCGGGC CTCCTCACCC ACTACCTGAC 
CCTCCCTCCC CGGCTACCCC TCTACCCGCA GCCCCACCTC CCGCTGTAGC CGCAGCCCCT 
CCCACCTTGG ACTCCACAGC ATCCAGCCTG ACCTCTTACG ACAGCGAAGT GGCCACACTG 
ACCCAGGGGG CTCCCGCGGC TCCTGGGGAT CCTCCAGCTC CAGGCCCGCC GGCCCCGGCA 
GCCCCAGCTC CCCCAGCACC GCAGCCAGGC CCTGATCCTC CACCTGGCAC AGATTCTGGA 
ATTGAGGAGG TGGACAGTCG GAGTAGCAGT GATCACCCGC TGGAGACCAT CAGCAGCGCA 
TCCACACTCA GCAGCTTGTC CGCCGAAGGA GGAGGCAATA CAGGGGGCGT GGCTGGGGGC 
GGGGCCGGGG TGGCCAGTGG GACGGAGCTC CTGGACACCT ACGTTGCCTA CCTGGATGGC 
CAGGCCTTCG GGGGCAGTGG AACTCCTGGC CCACCATATC CTCCCCAGCT CATGACTCCT 
TCTAAGCTCC GGGGCAGGGC GCTTGGGACT AGTGGAAATT TAAGACCTGG TCCCAGTGGG 
GGACTCCGAG ATCCTGTCAC CCCCACCAGC CCCACTGTCT CTGTGACCGG AGCTGGAACA 
GATGGGCTAC TGGCCTTGAG TGCTTGCCCT GGACCCTCAA CGGCAGGTGT GGCAGGGGGT 
CCCGTGGCAG TTGAGCCAGA AGTCCCGCCT GTACCTTTGC CCGCTGCCTC ATCCCTGCCT 
CGGAAGCTAT TACCGTGGGA GGAGGGTCCG GGACCACCAC CACCACCCCT GCCAGGGCCC 
CTGTCCCAGC CTCAGGCCTC TGCCTTGGCC ACGGTCAAAG CCAGCATCAT CAGCGAACTC 
AGCTCCAAGC TCCAGCAGTT TGGTGGGTCC TCTACCGCAG GAGGAGCTCT GCCTTGGGCT 
CGGGGAGGCA GCGGGGGCAG CACAGACAGC CACCACGGAG GAGCCAGTTA CATCCCAGAG 
AGGACGTCCT CCCTGCAGCG GCAGAGGCTC TCTGAAGACT CCCAGACCTC TCTCCTCTCC 
AAACCCAGCA GCAGCATCTT TCAGAACTGG CCTAAACCCC CTCTGCCACC ACTCCCCACT 
GGGTCTGGGG TCTCCTCTTC AACAGCTGCA GCCCCGGGAG CTACATCCCC TTCAGCCTCA 
TCTGCCTCAG CTTCCACCAG ACACCTGCAG GGTGTGGAAT TTGAGATGCG GCCTCCGCTG 
CTCCGCCGGG CACCCAGCCC CTCGCTGCTG CCTGCCTCGG ATCACAAGGT CAGCCCAGCG 
CCCAGGCCTT CCTCTTTGCC CATCCTGCCT TCAGGACCCA TCTACCCGGG CCTCTTCGAC 
ATCCGTAGCT CTCCAACCGG AGGGGCGGGA GGCTCGACTG ACCCCTTCGC TCCCGTCTTT 
GTGCCACCAC ACCCCGGGAT ATCTGGGGGT CTTGGAGGAG CCTTGTCTGG GGCCTCTCGC 
TCCCTATCAC CCACCCGCCT GCTCTCGCTG CCCCCGGACA AGCCGTTCGG CGCCAAGCCT 
CTAGGGTTCT GGACCAAGTT TGACGTCGCC GATTGGCTAG AGTGGCTGGG CTTGTCGGAG 
CACCGAGCCC AGTTCCTGGA CCACGAGATT GATGGCTCCC ATCTGCCTGC CTTGACCAAG 
GAGGACTATG TGGACCTGGG TGTAACCAGG GTGGGCCACC GTATGAACAT TGACCGGGCT 
CTCAAATTTT TCCTGGAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shank1 ( NM_031751.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113939.2 Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), mRNA.

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CloneID	ORa08651
Clone ID Related Accession (Same CDS sequence)	NM_031751.3
Accession Version	NM_031751.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	SH3 and multiple ankyrin repeat domains protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF159046.1. On Sep 1, 2016 this sequence version replaced NM_031751.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF159046.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTCT GATCGATGGG ATTGACTCAG GGAGTGATTA CATCATCAAG 
GAGAAGACAG TCTTACTGCA GAAGAAGGAC AGTGAGGGGT TCGGGTTCGT GCTCCGGGGG 
GCCAAGGCGC AGACCCCCAT CGAGGAATTC ACCCCCACCC CGGCCTTCCC GGCGCTGCAG 
TACCTCGAGT CTGTGGACGA GGGTGGCGTG GCATGGCGGG CTGGACTACG GATGGGAGAC 
TTCCTCATCG AGGTGAATGG ACAGAATGTA GTGAAGGTTG GCCACCGCCA GGTGGTCAAC 
ATGATCCGAC AGGGAGGCAA CACACTGATG GTCAAGGTGG TGATGGTGAC CAGACACCCA 
GACATGGACG AGGCTGTCCA CAAGAAAGCA TCCCAGCAGG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCC TGCGTTCCAA GTCTATGACC TCAGAGCTGG AGGAAATGGT CTCCCCCTGG 
AAGAAGAAAA TTGAATACGA GCAACAGCCC GCGGCAGTGC CCAGCATGGA GAAAAAGAGG 
ACTGTGTATC AGATGGCACT CAACAAACTG GATGAGATCT TGGCCGCTGC TCAGCAGACC 
ATCAGCGCAA GTGAGAGTCC TGGGCCTGGT GGCCTGGCAT CCCTGGGCAA GCACCGACCC 
AAAGGATTCT TTGCCACTGA GTCGAGCTTT GATCCTCACC ACCGCTCCCA GCCAAGTTAT 
GACCGGCCTT CTTTCCTGCC TCCTGGACCC GGCCTTATGC TCCGGCAGAA ATCTATCGGG 
GCTGCGGAAG ACGACAGACC CTACCTAGCA CCCCCAGCCA TGAAATTCAG TCGTAGCCTG 
TCAGTCCCCG GTTCAGAGGA CATACCCCCA CCACCCACTA CATCACCACC AGAGCCGCCC 
TATAGCACAC CTCCGGCCCC CTCCTCCTCC GGCCGCCTCA CTCCCTCGCC TCGGGGAGGG 
CCCTTTAACC CCAGTTCTGG GGGTCCCCTT CCCGCCTCCT CCCCTTCATC TTTCGATGGG 
CCATCTCCTC CTGACACCCG TGGTGGGGGC CGAGAAAAGA GCCTATATCA CAGCGCAGCC 
CTGCCCCCTG CCCACCACCA TCCACCTCAT CACCACCATC ATCACGCGCC ACCTCCCCAG 
CCCCACCATC ACCATGCACA TCCTCCTCAC CCACCGGAGA TGGAGACAGG AGGTTCCCCC 
GACGATCCCC CACCTCGATT GGCCCTGGGA CCCCAGCCCA GCCTTCGAGG CTGGCGAGGT 
GGAGGACCCA GCCCGACCTC GGGGGCCCCA TCACCTTCCC ACCACAGTAG CAGCGGAGGC 
AGCAGCGGCC CTACTCAGGC TCCCGCCCTG CGCTACTTCC AACTGCCCCC GAGGGCGGCC 
AGTGCGGCTA TGTATGTACC AGCACGTTCC GGCCGCGGTC GCAAGGGCCC ACTGGTCAAG 
CAGACCAAAG TGGAAGGCGA GCCCCAAAAG GGCAGCATCC CTTCCGCGTC CTCTCCAACA 
TCCCCGGCGT TGCCTCGGTC TGAACCACCT CCGGCCGGGC CGTCGGAGAA AAATTCCATC 
CCTATCCCGA CCATCATCAT CAAAGCCCCA TCTACCAGTA GCAGCGGCCG CAGCAGTCAG 
GGAAGCAGCA CAGAGGCTGA GCCCCCCACC CAGCCCGATG GCGCGGGCGG CGGCGGATCC 
TCGCCTAGCC CTGCACCTGC CACGTCCCCT GTGCCGCCAT CCCCGTCGCC CGTGCCCACT 
CCCGCGTCAC CCAGCGGCCC GGCCACCCTG GATTTCACTA GCCAGTTTGG AGCAGCCCTA 
GTAGGCGCGG CGCGGAGGGA GGGAGGCTGG CAGAACGAAG CCCGGCGGCG GTCCACGCTG 
TTCCTGTCCA CCGACGCAGG AGATGAGGAT GGAGGCGACA GCGGGCTGGG CCCGGGGGGG 
CCCCCGGGTC CCCGGCTGCG CCATTCCAAA TCCATTGACG AAGGCATGTT CTCCGCAGAG 
CCGTACCTCC GGCTGGAGTC GGGGGGCAGC AGTGGGGGCT ACGGGGCCTA CGCAGCGGGC 
AGCCGAGCCT ACGGGGGCAG CGGGAGCAGC AGCGCCTTCA CCAGCTTCCT CCCCCCGCGG 
CCCCTGGTGC ATCCGCTGAC CGGCAAAGCC TTGGATCCCG CCTCTCCGCT GGGGTTGGCC 
TTGGCCGCCC GGGAGCGGGC CTTGAAGGAG TCCTCGGAGG GTGGGGGCAC CCCTCAGCCG 
CCCCCGAGAC CTCCATCACC ACGCTACGAC GCCCCACCGC CTACCCTTCA CCACCACTCG 
CCCCATTCAC CCCATTCGCC ACATGCGCGT CACGAGCCAG TGTTGCGCCT CTGGGGGGAC 
CCCGCGCGCC GGGAGCTGGG GTACCGGGCA GGCCTAGGGA GTCAGGAGAA GGCCCTTACC 
GCCAGTCCAC CTGCAGCTCG CCGCTCCCTA CTGCACCGTC TGCCCCCGAC GGCTCCCGGG 
GTCGGGCCGC TCCTGCTGCA GCTGGGGCCG GAGCCCCCAA CTCCCCATCC TGGGGTGAGC 
AAGGCCTGGC GCACAGCAGC CCCGGAGGAG CCGGAGCGGC TGCCGCTGCA CGTGCGATTC 
CTTGAGAATT GCCAAGCCCG GCCTCCCCCA GCTGGCACGA GAGGGTCCTC TACTGAAGAT 
GGGCCTGGGG TCCCGCCTCC CAGCCCTCGC AGAGTCTTGC CAACCTCTCC CACCTCCCCA 
CGAGGCAACG AGGAGAACGG CCTTCCGCTA CTGGTCCTGC CACCGCCAGC CCCTTCGGTG 
GATGTGGACG ACGGCGAGTT CTTGTTTGCG GAGCCGCTGC CTCCACCCCT GGAGTTTTCC 
AATAGTTTCG AAAAGCCAGA GTCGCCTCTC ACACCCGGGC CTCCTCACCC ACTCCCTGAC 
CCTCCCTCCC CGGCTACCCC TCTACCCGCA GCCCCACCTC CCGCTGTAGC CGCAGCCCCT 
CCCACCTTGG ACTCCACAGC ATCCAGCCTG ACCTCTTACG ACAGCGAAGT GGCCACACTG 
ACCCAGGGGG CTCCCGCGGC TCCTGGGGAT CCTCCAGCTC CAGGCCCGCC GGCCCCGGCA 
GCCCCAGCTC CCCCAGCACC GCAGCCAGGC CCTGATCCTC CACCTGGCAC AGATTCTGGA 
ATTGAGGAGG TGGACAGTCG GAGTAGCAGT GATCACCCGC TGGAGACCAT CAGCAGCGCA 
TCCACACTCA GCAGCTTGTC CGCCGAAGGA GGAGGCAATA CAGGGGGCGT GGCTGGGGGC 
GGGGCCGGGG TGGCCAGTGG GACGGAGCTC CTGGACACCT ACGTTGCCTA CCTGGATGGC 
CAGGCCTTCG GGGGCAGTGG AACTCCTGGC CCACCATATC CTCCCCAGCT CATGACTCCT 
TCTAAGCTCC GGGGCAGGGC GCTTGGGACT AGTGGAAATT TAAGACCTGG TCCCAGTGGG 
GGACTCCGAG ATCCTGTCAC CCCCACCAGC CCCACTGTCT CTGTGACCGG AGCTGGAACA 
GATGGGCTAC TGGCCTTGAG TGCTTGCCCT GGACCCTCAA CGGCAGGTGT GGCAGGGGGT 
CCCGTGGCAG TTGAGCCAGA AGTCCCGCCT GTACCTTTGC CCGCTGCCTC ATCCCTGCCT 
CGGAAGCTAT TACCGTGGGA GGAGGGTCCG GGACCACCAC CACCACCCCT GCCAGGGCCC 
CTGTCCCAGC CTCAGGCCTC TGCCTTGGCC ACGGTCAAAG CCAGCATCAT CAGCGAACTC 
AGCTCCAAGC TCCAGCAGTT TGGTGGGTCC TCTACCGCAG GAGGAGCTCT GCCTTGGGCT 
CGGGGAGGCA GCGGGGGCAG CACAGACAGC CACCACGGAG GAGCCAGTTA CATCCCAGAG 
AGGACGTCCT CCCTGCAGCG GCAGAGGCTC TCTGAAGACT CCCAGACCTC TCTCCTCTCC 
AAACCCAGCA GCAGCATCTT TCAGAACTGG CCTAAACCCC CTCTGCCACC ACTCCCCACT 
GGGTCTGGGG TCTCCTCTTC AACAGCTGCA GCCCCGGGAG CTACATCCCC TTCAGCCTCA 
TCTGCCTCAG CTTCCACCAG ACACCTGCAA GGTGTGGAAT TTGAGATGCG GCCTCCGCTG 
CTCCGCCGGG CACCCAGCCC TTCGTTGCTG CCTGCTTCGG ATCACAAGGT CAGCCCAGCG 
CCCAGGCCTT CCTCTTTGCC CATCCTGCCT TCAGGACCCA TCTACCCGGG CCTCTTCGAC 
ATCCGTAGCT CTCCAACCGG AGGGGCGGGA GGCTCGACTG ACCCCTTCGC TCCCGTCTTT 
GTGCCACCAC ACCCCGGGAT ATCTGGGGGT CTTGGAGGAG CCTTGTCTGG GGCCTCTCGC 
TCCCTATCAC CCACCCGCCT GCTCTCGCTG CCCCCGGACA AGCCGTTCGG CGCCAAGCCT 
CTAGGGTTCT GGACCAAGTT TGACGTCGCC GATTGGCTAG AGTGGCTGGG CTTGTCGGAG 
CACCGAGCCC AGTTCCTGGA CCACGAGATT GATGGCTCCC ATCTGCCTGC CTTGACCAAG 
GAGGACTATG TGGACCTGGG TGTAACCAGG GTGGGCCACC GTATGAACAT TGACCGGGCT 
CTCAAATTTT TCCTGGAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113939.2
CDS	89..6592
Translation	MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPQGTRGRGSGAPGNLASTRGLQGRSMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKETCARILLYRGANKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARRRGPPGAGLTVPPALLRANSDTSMALPDWMVFSAPGASSSGTPGPTSGPQGQSQPSAPSTKLSSGTLRSASSPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPRDGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQAQGEGEISLSKGEKIKVLSIGEGGFWEGQVKGRVGWFPSDCLEEVANRSQEGRQESRSDKAKRLFRHYTVGSYDSFDAPSLIDGIDSGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKASQQAKRLPPPAISLRSKSMTSELEEMVSPWKKKIEYEQQPAAVPSMEKKRTVYQMALNKLDEILAAAQQTISASESPGPGGLASLGKHRPKGFFATESSFDPHHRSQPSYDRPSFLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPSSGGPLPASSPSSFDGPSPPDTRGGGREKSLYHSAALPPAHHHPPHHHHHHAPPPQPHHHHAHPPHPPEMETGGSPDDPPPRLALGPQPSLRGWRGGGPSPTSGAPSPSHHSSSGGSSGPTQAPALRYFQLPPRAASAAMYVPARSGRGRKGPLVKQTKVEGEPQKGSIPSASSPTSPALPRSEPPPAGPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQPDGAGGGGSSPSPAPATSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDAGDEDGGDSGLGPGGPPGPRLRHSKSIDEGMFSAEPYLRLESGGSSGGYGAYAAGSRAYGGSGSSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGTPQPPPRPPSPRYDAPPPTLHHHSPHSPHSPHARHEPVLRLWGDPARRELGYRAGLGSQEKALTASPPAARRSLLHRLPPTAPGVGPLLLQLGPEPPTPHPGVSKAWRTAAPEEPERLPLHVRFLENCQARPPPAGTRGSSTEDGPGVPPPSPRRVLPTSPTSPRGNEENGLPLLVLPPPAPSVDVDDGEFLFAEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPSPATPLPAAPPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPAPGPPAPAAPAPPAPQPGPDPPPGTDSGIEEVDSRSSSDHPLETISSASTLSSLSAEGGGNTGGVAGGGAGVASGTELLDTYVAYLDGQAFGGSGTPGPPYPPQLMTPSKLRGRALGTSGNLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLALSACPGPSTAGVAGGPVAVEPEVPPVPLPAASSLPRKLLPWEEGPGPPPPPLPGPLSQPQASALATVKASIISELSSKLQQFGGSSTAGGALPWARGGSGGSTDSHHGGASYIPERTSSLQRQRLSEDSQTSLLSKPSSSIFQNWPKPPLPPLPTGSGVSSSTAAAPGATSPSASSASASTRHLQGVEFEMRPPLLRRAPSPSLLPASDHKVSPAPRPSSLPILPSGPIYPGLFDIRSSPTGGAGGSTDPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKPLGFWTKFDVADWLEWLGLSEHRAQFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLER

Target ORF information:

RefSeq Version	NM_031751.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031751.3

ORF Insert Sequence:

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTCT GATCGATGGG ATTGACTCAG GGAGTGATTA CATCATCAAG 
GAGAAGACAG TCTTACTGCA GAAGAAGGAC AGTGAGGGGT TCGGGTTCGT GCTCCGGGGG 
GCCAAGGCGC AGACCCCCAT CGAGGAATTC ACCCCCACCC CGGCCTTCCC GGCGCTGCAG 
TACCTCGAGT CTGTGGACGA GGGTGGCGTG GCATGGCGGG CTGGACTACG GATGGGAGAC 
TTCCTCATCG AGGTGAATGG ACAGAATGTA GTGAAGGTTG GCCACCGCCA GGTGGTCAAC 
ATGATCCGAC AGGGAGGCAA CACACTGATG GTCAAGGTGG TGATGGTGAC CAGACACCCA 
GACATGGACG AGGCTGTCCA CAAGAAAGCA TCCCAGCAGG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCC TGCGTTCCAA GTCTATGACC TCAGAGCTGG AGGAAATGGT CTCCCCCTGG 
AAGAAGAAAA TTGAATACGA GCAACAGCCC GCGGCAGTGC CCAGCATGGA GAAAAAGAGG 
ACTGTGTATC AGATGGCACT CAACAAACTG GATGAGATCT TGGCCGCTGC TCAGCAGACC 
ATCAGCGCAA GTGAGAGTCC TGGGCCTGGT GGCCTGGCAT CCCTGGGCAA GCACCGACCC 
AAAGGATTCT TTGCCACTGA GTCGAGCTTT GATCCTCACC ACCGCTCCCA GCCAAGTTAT 
GACCGGCCTT CTTTCCTGCC TCCTGGACCC GGCCTTATGC TCCGGCAGAA ATCTATCGGG 
GCTGCGGAAG ACGACAGACC CTACCTAGCA CCCCCAGCCA TGAAATTCAG TCGTAGCCTG 
TCAGTCCCCG GTTCAGAGGA CATACCCCCA CCACCCACTA CATCACCACC AGAGCCGCCC 
TATAGCACAC CTCCGGCCCC CTCCTCCTCC GGCCGCCTCA CTCCCTCGCC TCGGGGAGGG 
CCCTTTAACC CCAGTTCTGG GGGTCCCCTT CCCGCCTCCT CCCCTTCATC TTTCGATGGG 
CCATCTCCTC CTGACACCCG TGGTGGGGGC CGAGAAAAGA GCCTATATCA CAGCGCAGCC 
CTGCCCCCTG CCCACCACCA TCCACCTCAT CACCACCATC ATCACGCGCC ACCTCCCCAG 
CCCCACCATC ACCATGCACA TCCTCCTCAC CCACCGGAGA TGGAGACAGG AGGTTCCCCC 
GACGATCCCC CACCTCGATT GGCCCTGGGA CCCCAGCCCA GCCTTCGAGG CTGGCGAGGT 
GGAGGACCCA GCCCGACCTC GGGGGCCCCA TCACCTTCCC ACCACAGTAG CAGCGGAGGC 
AGCAGCGGCC CTACTCAGGC TCCCGCCCTG CGCTACTTCC AACTGCCCCC GAGGGCGGCC 
AGTGCGGCTA TGTATGTACC AGCACGTTCC GGCCGCGGTC GCAAGGGCCC ACTGGTCAAG 
CAGACCAAAG TGGAAGGCGA GCCCCAAAAG GGCAGCATCC CTTCCGCGTC CTCTCCAACA 
TCCCCGGCGT TGCCTCGGTC TGAACCACCT CCGGCCGGGC CGTCGGAGAA AAATTCCATC 
CCTATCCCGA CCATCATCAT CAAAGCCCCA TCTACCAGTA GCAGCGGCCG CAGCAGTCAG 
GGAAGCAGCA CAGAGGCTGA GCCCCCCACC CAGCCCGATG GCGCGGGCGG CGGCGGATCC 
TCGCCTAGCC CTGCACCTGC CACGTCCCCT GTGCCGCCAT CCCCGTCGCC CGTGCCCACT 
CCCGCGTCAC CCAGCGGCCC GGCCACCCTG GATTTCACTA GCCAGTTTGG AGCAGCCCTA 
GTAGGCGCGG CGCGGAGGGA GGGAGGCTGG CAGAACGAAG CCCGGCGGCG GTCCACGCTG 
TTCCTGTCCA CCGACGCAGG AGATGAGGAT GGAGGCGACA GCGGGCTGGG CCCGGGGGGG 
CCCCCGGGTC CCCGGCTGCG CCATTCCAAA TCCATTGACG AAGGCATGTT CTCCGCAGAG 
CCGTACCTCC GGCTGGAGTC GGGGGGCAGC AGTGGGGGCT ACGGGGCCTA CGCAGCGGGC 
AGCCGAGCCT ACGGGGGCAG CGGGAGCAGC AGCGCCTTCA CCAGCTTCCT CCCCCCGCGG 
CCCCTGGTGC ATCCGCTGAC CGGCAAAGCC TTGGATCCCG CCTCTCCGCT GGGGTTGGCC 
TTGGCCGCCC GGGAGCGGGC CTTGAAGGAG TCCTCGGAGG GTGGGGGCAC CCCTCAGCCG 
CCCCCGAGAC CTCCATCACC ACGCTACGAC GCCCCACCGC CTACCCTTCA CCACCACTCG 
CCCCATTCAC CCCATTCGCC ACATGCGCGT CACGAGCCAG TGTTGCGCCT CTGGGGGGAC 
CCCGCGCGCC GGGAGCTGGG GTACCGGGCA GGCCTAGGGA GTCAGGAGAA GGCCCTTACC 
GCCAGTCCAC CTGCAGCTCG CCGCTCCCTA CTGCACCGTC TGCCCCCGAC GGCTCCCGGG 
GTCGGGCCGC TCCTGCTGCA GCTGGGGCCG GAGCCCCCAA CTCCCCATCC TGGGGTGAGC 
AAGGCCTGGC GCACAGCAGC CCCGGAGGAG CCGGAGCGGC TGCCGCTGCA CGTGCGATTC 
CTTGAGAATT GCCAAGCCCG GCCTCCCCCA GCTGGCACGA GAGGGTCCTC TACTGAAGAT 
GGGCCTGGGG TCCCGCCTCC CAGCCCTCGC AGAGTCTTGC CAACCTCTCC CACCTCCCCA 
CGAGGCAACG AGGAGAACGG CCTTCCGCTA CTGGTCCTGC CACCGCCAGC CCCTTCGGTG 
GATGTGGACG ACGGCGAGTT CTTGTTTGCG GAGCCGCTGC CTCCACCCCT GGAGTTTTCC 
AATAGTTTCG AAAAGCCAGA GTCGCCTCTC ACACCCGGGC CTCCTCACCC ACTCCCTGAC 
CCTCCCTCCC CGGCTACCCC TCTACCCGCA GCCCCACCTC CCGCTGTAGC CGCAGCCCCT 
CCCACCTTGG ACTCCACAGC ATCCAGCCTG ACCTCTTACG ACAGCGAAGT GGCCACACTG 
ACCCAGGGGG CTCCCGCGGC TCCTGGGGAT CCTCCAGCTC CAGGCCCGCC GGCCCCGGCA 
GCCCCAGCTC CCCCAGCACC GCAGCCAGGC CCTGATCCTC CACCTGGCAC AGATTCTGGA 
ATTGAGGAGG TGGACAGTCG GAGTAGCAGT GATCACCCGC TGGAGACCAT CAGCAGCGCA 
TCCACACTCA GCAGCTTGTC CGCCGAAGGA GGAGGCAATA CAGGGGGCGT GGCTGGGGGC 
GGGGCCGGGG TGGCCAGTGG GACGGAGCTC CTGGACACCT ACGTTGCCTA CCTGGATGGC 
CAGGCCTTCG GGGGCAGTGG AACTCCTGGC CCACCATATC CTCCCCAGCT CATGACTCCT 
TCTAAGCTCC GGGGCAGGGC GCTTGGGACT AGTGGAAATT TAAGACCTGG TCCCAGTGGG 
GGACTCCGAG ATCCTGTCAC CCCCACCAGC CCCACTGTCT CTGTGACCGG AGCTGGAACA 
GATGGGCTAC TGGCCTTGAG TGCTTGCCCT GGACCCTCAA CGGCAGGTGT GGCAGGGGGT 
CCCGTGGCAG TTGAGCCAGA AGTCCCGCCT GTACCTTTGC CCGCTGCCTC ATCCCTGCCT 
CGGAAGCTAT TACCGTGGGA GGAGGGTCCG GGACCACCAC CACCACCCCT GCCAGGGCCC 
CTGTCCCAGC CTCAGGCCTC TGCCTTGGCC ACGGTCAAAG CCAGCATCAT CAGCGAACTC 
AGCTCCAAGC TCCAGCAGTT TGGTGGGTCC TCTACCGCAG GAGGAGCTCT GCCTTGGGCT 
CGGGGAGGCA GCGGGGGCAG CACAGACAGC CACCACGGAG GAGCCAGTTA CATCCCAGAG 
AGGACGTCCT CCCTGCAGCG GCAGAGGCTC TCTGAAGACT CCCAGACCTC TCTCCTCTCC 
AAACCCAGCA GCAGCATCTT TCAGAACTGG CCTAAACCCC CTCTGCCACC ACTCCCCACT 
GGGTCTGGGG TCTCCTCTTC AACAGCTGCA GCCCCGGGAG CTACATCCCC TTCAGCCTCA 
TCTGCCTCAG CTTCCACCAG ACACCTGCAA GGTGTGGAAT TTGAGATGCG GCCTCCGCTG 
CTCCGCCGGG CACCCAGCCC TTCGTTGCTG CCTGCTTCGG ATCACAAGGT CAGCCCAGCG 
CCCAGGCCTT CCTCTTTGCC CATCCTGCCT TCAGGACCCA TCTACCCGGG CCTCTTCGAC 
ATCCGTAGCT CTCCAACCGG AGGGGCGGGA GGCTCGACTG ACCCCTTCGC TCCCGTCTTT 
GTGCCACCAC ACCCCGGGAT ATCTGGGGGT CTTGGAGGAG CCTTGTCTGG GGCCTCTCGC 
TCCCTATCAC CCACCCGCCT GCTCTCGCTG CCCCCGGACA AGCCGTTCGG CGCCAAGCCT 
CTAGGGTTCT GGACCAAGTT TGACGTCGCC GATTGGCTAG AGTGGCTGGG CTTGTCGGAG 
CACCGAGCCC AGTTCCTGGA CCACGAGATT GATGGCTCCC ATCTGCCTGC CTTGACCAAG 
GAGGACTATG TGGACCTGGG TGTAACCAGG GTGGGCCACC GTATGAACAT TGACCGGGCT 
CTCAAATTTT TCCTGGAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shank1 ( XM_017589759.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445248.1 Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X2, mRNA.

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CloneID	ORa22781
Clone ID Related Accession (Same CDS sequence)	XM_017589759.1
Accession Version	XM_017589759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTCT GATCGATGGG ATTGACTCAG GGAGTGATTA CATCATCAAG 
GAGAAGACAG TCTTACTGCA GAAGAAGGAC AGTGAGGGGT TCGGGTTCGT GCTCCGGGGG 
GCCAAGGCGC AGACCCCCAT CGAGGAATTC ACCCCCACCC CGGCCTTCCC GGCGCTGCAG 
TACCTCGAGT CTGTGGACGA GGGTGGCGTG GCATGGCGGG CTGGACTACG GATGGGAGAC 
TTCCTCATCG AGGTGAATGG ACAGAATGTA GTGAAGGTTG GCCACCGCCA GGTGGTCAAC 
ATGATCCGAC AGGGAGGCAA CACACTGATG GTCAAGGTGG TGATGGTGAC CAGACACCCA 
GACATGGACG AGGCTGTCCA CAAGAAAGCA TCCCAGCAGG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCC TGCGTTCCAA GTCTATGACC TCAGAGCTGG AGGAAATGGA ATACGAGCAA 
CAGCCCGCGG CAGTGCCCAG CATGGAGAAA AAGAGGACTG TGTATCAGAT GGCACTCAAC 
AAACTGGATG AGATCTTGGC CGCTGCTCAG CAGACCATCA GCGCAAGTGA GAGTCCTGGG 
CCTGGTGGCC TGGCATCCCT GGGCAAGCAC CGACCCAAAG GATTCTTTGC CACTGAGTCG 
AGCTTTGATC CTCACCACCG CTCCCAGCCA AGTTATGACC GGCCTTCTTT CCTGCCTCCT 
GGACCCGGCC TTATGCTCCG GCAGAAATCT ATCGGGGCTG CGGAAGACGA CAGACCCTAC 
CTAGCACCCC CAGCCATGAA ATTCAGTCGT AGCCTGTCAG TCCCCGGTTC AGAGGACATA 
CCCCCACCAC CCACTACATC ACCACCAGAG CCGCCCTATA GCACACCTCC GGCCCCCTCC 
TCCTCCGGCC GCCTCACTCC CTCGCCTCGG GGAGGGCCCT TTAACCCCAG TTCTGGGGGT 
CCCCTTCCCG CCTCCTCCCC TTCATCTTTC GATGGGCCAT CTCCTCCTGA CACCCGTGGT 
GGGGGCCGAG AAAAGAGCCT ATATCACAGC GCAGCCCTGC CCCCTGCCCA CCACCATCCA 
CCTCATCACC ACCATCATCA CGCGCCACCT CCCCAGCCCC ACCATCACCA TGCACATCCT 
CCTCACCCAC CGGAGATGGA GACAGGAGGT TCCCCCGACG ATCCCCCACC TCGATTGGCC 
CTGGGACCCC AGCCCAGCCT TCGAGGCTGG CGAGGTGGAG GACCCAGCCC GACCTCGGGG 
GCCCCATCAC CTTCCCACCA CAGTAGCAGC GGAGGCAGCA GCGGCCCTAC TCAGGCTCCC 
GCCCTGCGCT ACTTCCAACT GCCCCCGAGG GCGGCCAGTG CGGCTATGTA TGTACCAGCA 
CGTTCCGGCC GCGGTCGCAA GGGCCCACTG GTCAAGCAGA CCAAAGTGGA AGGCGAGCCC 
CAAAAGGGCA GCATCCCTTC CGCGTCCTCT CCAACATCCC CGGCGTTGCC TCGGTCTGAA 
CCACCTCCGG CCGGGCCGTC GGAGAAAAAT TCCATCCCTA TCCCGACCAT CATCATCAAA 
GCCCCATCTA CCAGTAGCAG CGGCCGCAGC AGTCAGGGAA GCAGCACAGA GGCTGAGCCC 
CCCACCCAGC CCGATGGCGC GGGCGGCGGC GGATCCTCGC CTAGCCCTGC ACCTGCCACG 
TCCCCTGTGC CGCCATCCCC GTCGCCCGTG CCCACTCCCG CGTCACCCAG CGGCCCGGCC 
ACCCTGGATT TCACTAGCCA GTTTGGAGCA GCCCTAGTAG GCGCGGCGCG GAGGGAGGGA 
GGCTGGCAGA ACGAAGCCCG GCGGCGGTCC ACGCTGTTCC TGTCCACCGA CGCAGGAGAT 
GAGGATGGAG GCGACAGCGG GCTGGGCCCG GGGGGGCCCC CGGGTCCCCG GCTGCGCCAT 
TCCAAATCCA TTGACGAAGG CATGTTCTCC GCAGAGCCGT ACCTCCGGCT GGAGTCAGGG 
GGCAGCAGTG GGGGCTACGG GGCCTACGCA GCGGGCAGCC GAGCCTACGG GGGCAGCGGG 
AGCAGCAGCG CCTTCACCAG CTTCCTCCCC CCGCGGCCCC TGGTGCATCC GCTGACCGGC 
AAAGCCTTGG ATCCCGCCTC TCCGCTGGGG TTGGCCTTGG CCGCCCGGGA GCGGGCCTTG 
AAGGAGTCCT CGGAGGGTGG GGGCACCCCT CAGCCGCCCC CGAGACCTCC ATCACCACGC 
TACGACGCCC CACCGCCTAC CCTTCACCAC CACTCGCCCC ATTCACCCCA TTCGCCACAT 
GCGCGTCACG AGCCAGTGTT GCGCCTCTGG GGGGACCCCG CGCGCCGGGA GCTGGGGTAC 
CGGGCAGGCC TAGGGAGTCA GGAGAAGGCC CTTACCGCCA GTCCACCTGC AGCTCGCCGC 
TCCCTACTGC ACCGTCTGCC CCCGACGGCT CCCGGGGTCG GGCCGCTCCT GCTGCAGCTG 
GGGCCGGAGC CCCCAACTCC CCATCCTGGG GTGAGCAAGG CCTGGCGCAC AGCAGCCCCG 
GAGGAGCCGG AGCGGCTGCC GCTGCACGTG CGATTCCTTG AGAATTGCCA AGCCCGGCCT 
CCCCCAGCTG GCACGAGAGG GTCCTCTACT GAAGATGGGC CTGGGGTCCC GCCTCCCAGC 
CCTCGCAGAG TCTTGCCAAC CTCTCCCACC TCCCCACGAG GCAACGAGGA GAACGGCCTT 
CCGCTACTGG TCCTGCCACC GCCAGCCCCT TCGGTGGATG TGGACGACGG CGAGTTCTTG 
TTTGCGGAGC CGCTGCCTCC ACCCCTGGAG TTTTCCAATA GTTTCGAAAA GCCAGAGTCG 
CCTCTCACAC CCGGGCCTCC TCACCCACTA CCTGACCCTC CCTCCCCGGC TACCCCTCTA 
CCCGCAGCCC CACCTCCCGC TGTAGCCGCA GCCCCTCCCA CCTTGGACTC CACAGCATCC 
AGCCTGACCT CTTACGACAG CGAAGTGGCC ACACTGACCC AGGGGGCTCC CGCGGCTCCT 
GGGGATCCTC CAGCTCCAGG CCCGCCGGCC CCGGCAGCCC CAGCTCCCCC AGCACCGCAG 
CCAGGCCCTG ATCCTCCACC TGGCACAGAT TCTGGAATTG AGGAGGTGGA CAGTCGGAGT 
AGCAGTGATC ACCCGCTGGA GACCATCAGC AGCGCATCCA CACTCAGCAG CTTGTCCGCC 
GAAGGAGGAG GCAATACAGG GGGCGTGGCT GGGGGCGGGG CCGGGGTGGC CAGTGGGACG 
GAGCTCCTGG ACACCTACGT TGCCTACCTG GATGGCCAGG CCTTCGGGGG CAGTGGAACT 
CCTGGCCCAC CATATCCTCC CCAGCTCATG ACTCCTTCTA AGCTCCGGGG CAGGGCGCTT 
GGGACTAGTG GAAATTTAAG ACCTGGTCCC AGTGGGGGAC TCCGAGATCC TGTCACCCCC 
ACCAGCCCCA CTGTCTCTGT GACCGGAGCT GGAACAGATG GGCTACTGGC CTTGAGTGCT 
TGCCCTGGAC CCTCAACGGC AGGTGTGGCA GGGGGTCCCG TGGCAGTTGA GCCAGAAGTC 
CCGCCTGTAC CTTTGCCCGC TGCCTCATCC CTGCCTCGGA AGCTATTACC GTGGGAGGAG 
GGTCCGGGAC CACCACCACC ACCCCTGCCA GGGCCCCTGT CCCAGCCTCA GGCCTCTGCC 
TTGGCCACGG TCAAAGCCAG CATCATCAGC GAACTCAGCT CCAAGCTCCA GCAGTTTGGT 
GGGTCCTCTA CCGCAGGAGG AGCTCTGCCT TGGGCTCGGG GAGGCAGCGG GGGCAGCACA 
GACAGCCACC ACGGAGGAGC CAGTTACATC CCAGAGAGGA CGTCCTCCCT GCAGCGGCAG 
AGGCTCTCTG AAGACTCCCA GACCTCTCTC CTCTCCAAAC CCAGCAGCAG CATCTTTCAG 
AACTGGCCTA AACCCCCTCT GCCACCACTC CCCACTGGGT CTGGGGTCTC CTCTTCAACA 
GCTGCAGCCC CGGGAGCTAC ATCCCCTTCA GCCTCATCTG CCTCAGCTTC CACCAGACAC 
CTGCAGGGTG TGGAATTTGA GATGCGGCCT CCGCTGCTCC GCCGGGCACC CAGCCCCTCG 
CTGCTGCCTG CCTCGGATCA CAAGGTCAGC CCAGCGCCCA GGCCTTCCTC TTTGCCCATC 
CTGCCTTCAG GACCCATCTA CCCGGGCCTC TTCGACATCC GTAGCTCTCC AACCGGAGGG 
GCGGGAGGCT CGACTGACCC CTTCGCTCCC GTCTTTGTGC CACCACACCC CGGGATATCT 
GGGGGTCTTG GAGGAGCCTT GTCTGGGGCC TCTCGCTCCC TATCACCCAC CCGCCTGCTC 
TCGCTGCCCC CGGACAAGCC GTTCGGCGCC AAGCCTCTAG GGTTCTGGAC CAAGTTTGAC 
GTCGCCGATT GGCTAGAGTG GCTGGGCTTG TCGGAGCACC GAGCCCAGTT CCTGGACCAC 
GAGATTGATG GCTCCCATCT GCCTGCCTTG ACCAAGGAGG ACTATGTGGA CCTGGGTGTA 
ACCAGGGTGG GCCACCGTAT GAACATTGAC CGGGCTCTCA AATTTTTCCT GGAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445248.1
CDS	102..6581
Translation	MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPQGTRGRGSGAPGNLASTRGLQGRSMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKETCARILLYRGANKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARRRGPPGAGLTVPPALLRANSDTSMALPDWMVFSAPGASSSGTPGPTSGPQGQSQPSAPSTKLSSGTLRSASSPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPRDGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQAQGEGEISLSKGEKIKVLSIGEGGFWEGQVKGRVGWFPSDCLEEVANRSQEGRQESRSDKAKRLFRHYTVGSYDSFDAPSLIDGIDSGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKASQQAKRLPPPAISLRSKSMTSELEEMEYEQQPAAVPSMEKKRTVYQMALNKLDEILAAAQQTISASESPGPGGLASLGKHRPKGFFATESSFDPHHRSQPSYDRPSFLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPSSGGPLPASSPSSFDGPSPPDTRGGGREKSLYHSAALPPAHHHPPHHHHHHAPPPQPHHHHAHPPHPPEMETGGSPDDPPPRLALGPQPSLRGWRGGGPSPTSGAPSPSHHSSSGGSSGPTQAPALRYFQLPPRAASAAMYVPARSGRGRKGPLVKQTKVEGEPQKGSIPSASSPTSPALPRSEPPPAGPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQPDGAGGGGSSPSPAPATSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDAGDEDGGDSGLGPGGPPGPRLRHSKSIDEGMFSAEPYLRLESGGSSGGYGAYAAGSRAYGGSGSSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGTPQPPPRPPSPRYDAPPPTLHHHSPHSPHSPHARHEPVLRLWGDPARRELGYRAGLGSQEKALTASPPAARRSLLHRLPPTAPGVGPLLLQLGPEPPTPHPGVSKAWRTAAPEEPERLPLHVRFLENCQARPPPAGTRGSSTEDGPGVPPPSPRRVLPTSPTSPRGNEENGLPLLVLPPPAPSVDVDDGEFLFAEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPSPATPLPAAPPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPAPGPPAPAAPAPPAPQPGPDPPPGTDSGIEEVDSRSSSDHPLETISSASTLSSLSAEGGGNTGGVAGGGAGVASGTELLDTYVAYLDGQAFGGSGTPGPPYPPQLMTPSKLRGRALGTSGNLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLALSACPGPSTAGVAGGPVAVEPEVPPVPLPAASSLPRKLLPWEEGPGPPPPPLPGPLSQPQASALATVKASIISELSSKLQQFGGSSTAGGALPWARGGSGGSTDSHHGGASYIPERTSSLQRQRLSEDSQTSLLSKPSSSIFQNWPKPPLPPLPTGSGVSSSTAAAPGATSPSASSASASTRHLQGVEFEMRPPLLRRAPSPSLLPASDHKVSPAPRPSSLPILPSGPIYPGLFDIRSSPTGGAGGSTDPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKPLGFWTKFDVADWLEWLGLSEHRAQFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLER

Target ORF information:

RefSeq Version	XM_017589759.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589759.1

ORF Insert Sequence:

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTCT GATCGATGGG ATTGACTCAG GGAGTGATTA CATCATCAAG 
GAGAAGACAG TCTTACTGCA GAAGAAGGAC AGTGAGGGGT TCGGGTTCGT GCTCCGGGGG 
GCCAAGGCGC AGACCCCCAT CGAGGAATTC ACCCCCACCC CGGCCTTCCC GGCGCTGCAG 
TACCTCGAGT CTGTGGACGA GGGTGGCGTG GCATGGCGGG CTGGACTACG GATGGGAGAC 
TTCCTCATCG AGGTGAATGG ACAGAATGTA GTGAAGGTTG GCCACCGCCA GGTGGTCAAC 
ATGATCCGAC AGGGAGGCAA CACACTGATG GTCAAGGTGG TGATGGTGAC CAGACACCCA 
GACATGGACG AGGCTGTCCA CAAGAAAGCA TCCCAGCAGG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCC TGCGTTCCAA GTCTATGACC TCAGAGCTGG AGGAAATGGA ATACGAGCAA 
CAGCCCGCGG CAGTGCCCAG CATGGAGAAA AAGAGGACTG TGTATCAGAT GGCACTCAAC 
AAACTGGATG AGATCTTGGC CGCTGCTCAG CAGACCATCA GCGCAAGTGA GAGTCCTGGG 
CCTGGTGGCC TGGCATCCCT GGGCAAGCAC CGACCCAAAG GATTCTTTGC CACTGAGTCG 
AGCTTTGATC CTCACCACCG CTCCCAGCCA AGTTATGACC GGCCTTCTTT CCTGCCTCCT 
GGACCCGGCC TTATGCTCCG GCAGAAATCT ATCGGGGCTG CGGAAGACGA CAGACCCTAC 
CTAGCACCCC CAGCCATGAA ATTCAGTCGT AGCCTGTCAG TCCCCGGTTC AGAGGACATA 
CCCCCACCAC CCACTACATC ACCACCAGAG CCGCCCTATA GCACACCTCC GGCCCCCTCC 
TCCTCCGGCC GCCTCACTCC CTCGCCTCGG GGAGGGCCCT TTAACCCCAG TTCTGGGGGT 
CCCCTTCCCG CCTCCTCCCC TTCATCTTTC GATGGGCCAT CTCCTCCTGA CACCCGTGGT 
GGGGGCCGAG AAAAGAGCCT ATATCACAGC GCAGCCCTGC CCCCTGCCCA CCACCATCCA 
CCTCATCACC ACCATCATCA CGCGCCACCT CCCCAGCCCC ACCATCACCA TGCACATCCT 
CCTCACCCAC CGGAGATGGA GACAGGAGGT TCCCCCGACG ATCCCCCACC TCGATTGGCC 
CTGGGACCCC AGCCCAGCCT TCGAGGCTGG CGAGGTGGAG GACCCAGCCC GACCTCGGGG 
GCCCCATCAC CTTCCCACCA CAGTAGCAGC GGAGGCAGCA GCGGCCCTAC TCAGGCTCCC 
GCCCTGCGCT ACTTCCAACT GCCCCCGAGG GCGGCCAGTG CGGCTATGTA TGTACCAGCA 
CGTTCCGGCC GCGGTCGCAA GGGCCCACTG GTCAAGCAGA CCAAAGTGGA AGGCGAGCCC 
CAAAAGGGCA GCATCCCTTC CGCGTCCTCT CCAACATCCC CGGCGTTGCC TCGGTCTGAA 
CCACCTCCGG CCGGGCCGTC GGAGAAAAAT TCCATCCCTA TCCCGACCAT CATCATCAAA 
GCCCCATCTA CCAGTAGCAG CGGCCGCAGC AGTCAGGGAA GCAGCACAGA GGCTGAGCCC 
CCCACCCAGC CCGATGGCGC GGGCGGCGGC GGATCCTCGC CTAGCCCTGC ACCTGCCACG 
TCCCCTGTGC CGCCATCCCC GTCGCCCGTG CCCACTCCCG CGTCACCCAG CGGCCCGGCC 
ACCCTGGATT TCACTAGCCA GTTTGGAGCA GCCCTAGTAG GCGCGGCGCG GAGGGAGGGA 
GGCTGGCAGA ACGAAGCCCG GCGGCGGTCC ACGCTGTTCC TGTCCACCGA CGCAGGAGAT 
GAGGATGGAG GCGACAGCGG GCTGGGCCCG GGGGGGCCCC CGGGTCCCCG GCTGCGCCAT 
TCCAAATCCA TTGACGAAGG CATGTTCTCC GCAGAGCCGT ACCTCCGGCT GGAGTCAGGG 
GGCAGCAGTG GGGGCTACGG GGCCTACGCA GCGGGCAGCC GAGCCTACGG GGGCAGCGGG 
AGCAGCAGCG CCTTCACCAG CTTCCTCCCC CCGCGGCCCC TGGTGCATCC GCTGACCGGC 
AAAGCCTTGG ATCCCGCCTC TCCGCTGGGG TTGGCCTTGG CCGCCCGGGA GCGGGCCTTG 
AAGGAGTCCT CGGAGGGTGG GGGCACCCCT CAGCCGCCCC CGAGACCTCC ATCACCACGC 
TACGACGCCC CACCGCCTAC CCTTCACCAC CACTCGCCCC ATTCACCCCA TTCGCCACAT 
GCGCGTCACG AGCCAGTGTT GCGCCTCTGG GGGGACCCCG CGCGCCGGGA GCTGGGGTAC 
CGGGCAGGCC TAGGGAGTCA GGAGAAGGCC CTTACCGCCA GTCCACCTGC AGCTCGCCGC 
TCCCTACTGC ACCGTCTGCC CCCGACGGCT CCCGGGGTCG GGCCGCTCCT GCTGCAGCTG 
GGGCCGGAGC CCCCAACTCC CCATCCTGGG GTGAGCAAGG CCTGGCGCAC AGCAGCCCCG 
GAGGAGCCGG AGCGGCTGCC GCTGCACGTG CGATTCCTTG AGAATTGCCA AGCCCGGCCT 
CCCCCAGCTG GCACGAGAGG GTCCTCTACT GAAGATGGGC CTGGGGTCCC GCCTCCCAGC 
CCTCGCAGAG TCTTGCCAAC CTCTCCCACC TCCCCACGAG GCAACGAGGA GAACGGCCTT 
CCGCTACTGG TCCTGCCACC GCCAGCCCCT TCGGTGGATG TGGACGACGG CGAGTTCTTG 
TTTGCGGAGC CGCTGCCTCC ACCCCTGGAG TTTTCCAATA GTTTCGAAAA GCCAGAGTCG 
CCTCTCACAC CCGGGCCTCC TCACCCACTA CCTGACCCTC CCTCCCCGGC TACCCCTCTA 
CCCGCAGCCC CACCTCCCGC TGTAGCCGCA GCCCCTCCCA CCTTGGACTC CACAGCATCC 
AGCCTGACCT CTTACGACAG CGAAGTGGCC ACACTGACCC AGGGGGCTCC CGCGGCTCCT 
GGGGATCCTC CAGCTCCAGG CCCGCCGGCC CCGGCAGCCC CAGCTCCCCC AGCACCGCAG 
CCAGGCCCTG ATCCTCCACC TGGCACAGAT TCTGGAATTG AGGAGGTGGA CAGTCGGAGT 
AGCAGTGATC ACCCGCTGGA GACCATCAGC AGCGCATCCA CACTCAGCAG CTTGTCCGCC 
GAAGGAGGAG GCAATACAGG GGGCGTGGCT GGGGGCGGGG CCGGGGTGGC CAGTGGGACG 
GAGCTCCTGG ACACCTACGT TGCCTACCTG GATGGCCAGG CCTTCGGGGG CAGTGGAACT 
CCTGGCCCAC CATATCCTCC CCAGCTCATG ACTCCTTCTA AGCTCCGGGG CAGGGCGCTT 
GGGACTAGTG GAAATTTAAG ACCTGGTCCC AGTGGGGGAC TCCGAGATCC TGTCACCCCC 
ACCAGCCCCA CTGTCTCTGT GACCGGAGCT GGAACAGATG GGCTACTGGC CTTGAGTGCT 
TGCCCTGGAC CCTCAACGGC AGGTGTGGCA GGGGGTCCCG TGGCAGTTGA GCCAGAAGTC 
CCGCCTGTAC CTTTGCCCGC TGCCTCATCC CTGCCTCGGA AGCTATTACC GTGGGAGGAG 
GGTCCGGGAC CACCACCACC ACCCCTGCCA GGGCCCCTGT CCCAGCCTCA GGCCTCTGCC 
TTGGCCACGG TCAAAGCCAG CATCATCAGC GAACTCAGCT CCAAGCTCCA GCAGTTTGGT 
GGGTCCTCTA CCGCAGGAGG AGCTCTGCCT TGGGCTCGGG GAGGCAGCGG GGGCAGCACA 
GACAGCCACC ACGGAGGAGC CAGTTACATC CCAGAGAGGA CGTCCTCCCT GCAGCGGCAG 
AGGCTCTCTG AAGACTCCCA GACCTCTCTC CTCTCCAAAC CCAGCAGCAG CATCTTTCAG 
AACTGGCCTA AACCCCCTCT GCCACCACTC CCCACTGGGT CTGGGGTCTC CTCTTCAACA 
GCTGCAGCCC CGGGAGCTAC ATCCCCTTCA GCCTCATCTG CCTCAGCTTC CACCAGACAC 
CTGCAGGGTG TGGAATTTGA GATGCGGCCT CCGCTGCTCC GCCGGGCACC CAGCCCCTCG 
CTGCTGCCTG CCTCGGATCA CAAGGTCAGC CCAGCGCCCA GGCCTTCCTC TTTGCCCATC 
CTGCCTTCAG GACCCATCTA CCCGGGCCTC TTCGACATCC GTAGCTCTCC AACCGGAGGG 
GCGGGAGGCT CGACTGACCC CTTCGCTCCC GTCTTTGTGC CACCACACCC CGGGATATCT 
GGGGGTCTTG GAGGAGCCTT GTCTGGGGCC TCTCGCTCCC TATCACCCAC CCGCCTGCTC 
TCGCTGCCCC CGGACAAGCC GTTCGGCGCC AAGCCTCTAG GGTTCTGGAC CAAGTTTGAC 
GTCGCCGATT GGCTAGAGTG GCTGGGCTTG TCGGAGCACC GAGCCCAGTT CCTGGACCAC 
GAGATTGATG GCTCCCATCT GCCTGCCTTG ACCAAGGAGG ACTATGTGGA CCTGGGTGTA 
ACCAGGGTGG GCCACCGTAT GAACATTGAC CGGGCTCTCA AATTTTTCCT GGAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shank1 ( XM_017589760.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445249.1 Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X3, mRNA.

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CloneID	ORa22782
Clone ID Related Accession (Same CDS sequence)	XM_017589760.1
Accession Version	XM_017589760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTGA TTACATCATC AAGGAGAAGA CAGTCTTACT GCAGAAGAAG 
GACAGTGAGG GGTTCGGGTT CGTGCTCCGG GGGGCCAAGG CGCAGACCCC CATCGAGGAA 
TTCACCCCCA CCCCGGCCTT CCCGGCGCTG CAGTACCTCG AGTCTGTGGA CGAGGGTGGC 
GTGGCATGGC GGGCTGGACT ACGGATGGGA GACTTCCTCA TCGAGGTGAA TGGACAGAAT 
GTAGTGAAGG TTGGCCACCG CCAGGTGGTC AACATGATCC GACAGGGAGG CAACACACTG 
ATGGTCAAGG TGGTGATGGT GACCAGACAC CCAGACATGG ACGAGGCTGT CCACAAGAAA 
GCATCCCAGC AGGCTAAGCG GCTCCCGCCC CCAGCCATCT CCCTGCGTTC CAAGTCTATG 
ACCTCAGAGC TGGAGGAAAT GGTCTCCCCC TGGAAGAAGA AAATTGAATA CGAGCAACAG 
CCCGCGGCAG TGCCCAGCAT GGAGAAAAAG AGGACTGTGT ATCAGATGGC ACTCAACAAA 
CTGGATGAGA TCTTGGCCGC TGCTCAGCAG ACCATCAGCG CAAGTGAGAG TCCTGGGCCT 
GGTGGCCTGG CATCCCTGGG CAAGCACCGA CCCAAAGGAT TCTTTGCCAC TGAGTCGAGC 
TTTGATCCTC ACCACCGCTC CCAGCCAAGT TATGACCGGC CTTCTTTCCT GCCTCCTGGA 
CCCGGCCTTA TGCTCCGGCA GAAATCTATC GGGGCTGCGG AAGACGACAG ACCCTACCTA 
GCACCCCCAG CCATGAAATT CAGTCGTAGC CTGTCAGTCC CCGGTTCAGA GGACATACCC 
CCACCACCCA CTACATCACC ACCAGAGCCG CCCTATAGCA CACCTCCGGC CCCCTCCTCC 
TCCGGCCGCC TCACTCCCTC GCCTCGGGGA GGGCCCTTTA ACCCCAGTTC TGGGGGTCCC 
CTTCCCGCCT CCTCCCCTTC ATCTTTCGAT GGGCCATCTC CTCCTGACAC CCGTGGTGGG 
GGCCGAGAAA AGAGCCTATA TCACAGCGCA GCCCTGCCCC CTGCCCACCA CCATCCACCT 
CATCACCACC ATCATCACGC GCCACCTCCC CAGCCCCACC ATCACCATGC ACATCCTCCT 
CACCCACCGG AGATGGAGAC AGGAGGTTCC CCCGACGATC CCCCACCTCG ATTGGCCCTG 
GGACCCCAGC CCAGCCTTCG AGGCTGGCGA GGTGGAGGAC CCAGCCCGAC CTCGGGGGCC 
CCATCACCTT CCCACCACAG TAGCAGCGGA GGCAGCAGCG GCCCTACTCA GGCTCCCGCC 
CTGCGCTACT TCCAACTGCC CCCGAGGGCG GCCAGTGCGG CTATGTATGT ACCAGCACGT 
TCCGGCCGCG GTCGCAAGGG CCCACTGGTC AAGCAGACCA AAGTGGAAGG CGAGCCCCAA 
AAGGGCAGCA TCCCTTCCGC GTCCTCTCCA ACATCCCCGG CGTTGCCTCG GTCTGAACCA 
CCTCCGGCCG GGCCGTCGGA GAAAAATTCC ATCCCTATCC CGACCATCAT CATCAAAGCC 
CCATCTACCA GTAGCAGCGG CCGCAGCAGT CAGGGAAGCA GCACAGAGGC TGAGCCCCCC 
ACCCAGCCCG ATGGCGCGGG CGGCGGCGGA TCCTCGCCTA GCCCTGCACC TGCCACGTCC 
CCTGTGCCGC CATCCCCGTC GCCCGTGCCC ACTCCCGCGT CACCCAGCGG CCCGGCCACC 
CTGGATTTCA CTAGCCAGTT TGGAGCAGCC CTAGTAGGCG CGGCGCGGAG GGAGGGAGGC 
TGGCAGAACG AAGCCCGGCG GCGGTCCACG CTGTTCCTGT CCACCGACGC AGGAGATGAG 
GATGGAGGCG ACAGCGGGCT GGGCCCGGGG GGGCCCCCGG GTCCCCGGCT GCGCCATTCC 
AAATCCATTG ACGAAGGCAT GTTCTCCGCA GAGCCGTACC TCCGGCTGGA GTCAGGGGGC 
AGCAGTGGGG GCTACGGGGC CTACGCAGCG GGCAGCCGAG CCTACGGGGG CAGCGGGAGC 
AGCAGCGCCT TCACCAGCTT CCTCCCCCCG CGGCCCCTGG TGCATCCGCT GACCGGCAAA 
GCCTTGGATC CCGCCTCTCC GCTGGGGTTG GCCTTGGCCG CCCGGGAGCG GGCCTTGAAG 
GAGTCCTCGG AGGGTGGGGG CACCCCTCAG CCGCCCCCGA GACCTCCATC ACCACGCTAC 
GACGCCCCAC CGCCTACCCT TCACCACCAC TCGCCCCATT CACCCCATTC GCCACATGCG 
CGTCACGAGC CAGTGTTGCG CCTCTGGGGG GACCCCGCGC GCCGGGAGCT GGGGTACCGG 
GCAGGCCTAG GGAGTCAGGA GAAGGCCCTT ACCGCCAGTC CACCTGCAGC TCGCCGCTCC 
CTACTGCACC GTCTGCCCCC GACGGCTCCC GGGGTCGGGC CGCTCCTGCT GCAGCTGGGG 
CCGGAGCCCC CAACTCCCCA TCCTGGGGTG AGCAAGGCCT GGCGCACAGC AGCCCCGGAG 
GAGCCGGAGC GGCTGCCGCT GCACGTGCGA TTCCTTGAGA ATTGCCAAGC CCGGCCTCCC 
CCAGCTGGCA CGAGAGGGTC CTCTACTGAA GATGGGCCTG GGGTCCCGCC TCCCAGCCCT 
CGCAGAGTCT TGCCAACCTC TCCCACCTCC CCACGAGGCA ACGAGGAGAA CGGCCTTCCG 
CTACTGGTCC TGCCACCGCC AGCCCCTTCG GTGGATGTGG ACGACGGCGA GTTCTTGTTT 
GCGGAGCCGC TGCCTCCACC CCTGGAGTTT TCCAATAGTT TCGAAAAGCC AGAGTCGCCT 
CTCACACCCG GGCCTCCTCA CCCACTACCT GACCCTCCCT CCCCGGCTAC CCCTCTACCC 
GCAGCCCCAC CTCCCGCTGT AGCCGCAGCC CCTCCCACCT TGGACTCCAC AGCATCCAGC 
CTGACCTCTT ACGACAGCGA AGTGGCCACA CTGACCCAGG GGGCTCCCGC GGCTCCTGGG 
GATCCTCCAG CTCCAGGCCC GCCGGCCCCG GCAGCCCCAG CTCCCCCAGC ACCGCAGCCA 
GGCCCTGATC CTCCACCTGG CACAGATTCT GGAATTGAGG AGGTGGACAG TCGGAGTAGC 
AGTGATCACC CGCTGGAGAC CATCAGCAGC GCATCCACAC TCAGCAGCTT GTCCGCCGAA 
GGAGGAGGCA ATACAGGGGG CGTGGCTGGG GGCGGGGCCG GGGTGGCCAG TGGGACGGAG 
CTCCTGGACA CCTACGTTGC CTACCTGGAT GGCCAGGCCT TCGGGGGCAG TGGAACTCCT 
GGCCCACCAT ATCCTCCCCA GCTCATGACT CCTTCTAAGC TCCGGGGCAG GGCGCTTGGG 
ACTAGTGGAA ATTTAAGACC TGGTCCCAGT GGGGGACTCC GAGATCCTGT CACCCCCACC 
AGCCCCACTG TCTCTGTGAC CGGAGCTGGA ACAGATGGGC TACTGGCCTT GAGTGCTTGC 
CCTGGACCCT CAACGGCAGG TGTGGCAGGG GGTCCCGTGG CAGTTGAGCC AGAAGTCCCG 
CCTGTACCTT TGCCCGCTGC CTCATCCCTG CCTCGGAAGC TATTACCGTG GGAGGAGGGT 
CCGGGACCAC CACCACCACC CCTGCCAGGG CCCCTGTCCC AGCCTCAGGC CTCTGCCTTG 
GCCACGGTCA AAGCCAGCAT CATCAGCGAA CTCAGCTCCA AGCTCCAGCA GTTTGGTGGG 
TCCTCTACCG CAGGAGGAGC TCTGCCTTGG GCTCGGGGAG GCAGCGGGGG CAGCACAGAC 
AGCCACCACG GAGGAGCCAG TTACATCCCA GAGAGGACGT CCTCCCTGCA GCGGCAGAGG 
CTCTCTGAAG ACTCCCAGAC CTCTCTCCTC TCCAAACCCA GCAGCAGCAT CTTTCAGAAC 
TGGCCTAAAC CCCCTCTGCC ACCACTCCCC ACTGGGTCTG GGGTCTCCTC TTCAACAGCT 
GCAGCCCCGG GAGCTACATC CCCTTCAGCC TCATCTGCCT CAGCTTCCAC CAGACACCTG 
CAGGGTGTGG AATTTGAGAT GCGGCCTCCG CTGCTCCGCC GGGCACCCAG CCCCTCGCTG 
CTGCCTGCCT CGGATCACAA GGTCAGCCCA GCGCCCAGGC CTTCCTCTTT GCCCATCCTG 
CCTTCAGGAC CCATCTACCC GGGCCTCTTC GACATCCGTA GCTCTCCAAC CGGAGGGGCG 
GGAGGCTCGA CTGACCCCTT CGCTCCCGTC TTTGTGCCAC CACACCCCGG GATATCTGGG 
GGTCTTGGAG GAGCCTTGTC TGGGGCCTCT CGCTCCCTAT CACCCACCCG CCTGCTCTCG 
CTGCCCCCGG ACAAGCCGTT CGGCGCCAAG CCTCTAGGGT TCTGGACCAA GTTTGACGTC 
GCCGATTGGC TAGAGTGGCT GGGCTTGTCG GAGCACCGAG CCCAGTTCCT GGACCACGAG 
ATTGATGGCT CCCATCTGCC TGCCTTGACC AAGGAGGACT ATGTGGACCT GGGTGTAACC 
AGGGTGGGCC ACCGTATGAA CATTGACCGG GCTCTCAAAT TTTTCCTGGA GAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445249.1
CDS	102..6578
Translation	MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPQGTRGRGSGAPGNLASTRGLQGRSMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKETCARILLYRGANKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARRRGPPGAGLTVPPALLRANSDTSMALPDWMVFSAPGASSSGTPGPTSGPQGQSQPSAPSTKLSSGTLRSASSPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPRDGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQAQGEGEISLSKGEKIKVLSIGEGGFWEGQVKGRVGWFPSDCLEEVANRSQEGRQESRSDKAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKASQQAKRLPPPAISLRSKSMTSELEEMVSPWKKKIEYEQQPAAVPSMEKKRTVYQMALNKLDEILAAAQQTISASESPGPGGLASLGKHRPKGFFATESSFDPHHRSQPSYDRPSFLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPSSGGPLPASSPSSFDGPSPPDTRGGGREKSLYHSAALPPAHHHPPHHHHHHAPPPQPHHHHAHPPHPPEMETGGSPDDPPPRLALGPQPSLRGWRGGGPSPTSGAPSPSHHSSSGGSSGPTQAPALRYFQLPPRAASAAMYVPARSGRGRKGPLVKQTKVEGEPQKGSIPSASSPTSPALPRSEPPPAGPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQPDGAGGGGSSPSPAPATSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDAGDEDGGDSGLGPGGPPGPRLRHSKSIDEGMFSAEPYLRLESGGSSGGYGAYAAGSRAYGGSGSSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGTPQPPPRPPSPRYDAPPPTLHHHSPHSPHSPHARHEPVLRLWGDPARRELGYRAGLGSQEKALTASPPAARRSLLHRLPPTAPGVGPLLLQLGPEPPTPHPGVSKAWRTAAPEEPERLPLHVRFLENCQARPPPAGTRGSSTEDGPGVPPPSPRRVLPTSPTSPRGNEENGLPLLVLPPPAPSVDVDDGEFLFAEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPSPATPLPAAPPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPAPGPPAPAAPAPPAPQPGPDPPPGTDSGIEEVDSRSSSDHPLETISSASTLSSLSAEGGGNTGGVAGGGAGVASGTELLDTYVAYLDGQAFGGSGTPGPPYPPQLMTPSKLRGRALGTSGNLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLALSACPGPSTAGVAGGPVAVEPEVPPVPLPAASSLPRKLLPWEEGPGPPPPPLPGPLSQPQASALATVKASIISELSSKLQQFGGSSTAGGALPWARGGSGGSTDSHHGGASYIPERTSSLQRQRLSEDSQTSLLSKPSSSIFQNWPKPPLPPLPTGSGVSSSTAAAPGATSPSASSASASTRHLQGVEFEMRPPLLRRAPSPSLLPASDHKVSPAPRPSSLPILPSGPIYPGLFDIRSSPTGGAGGSTDPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKPLGFWTKFDVADWLEWLGLSEHRAQFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLER

Target ORF information:

RefSeq Version	XM_017589760.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SH3 and multiple ankyrin repeat domains 1 (Shank1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589760.1

ORF Insert Sequence:

ATGACCCACA GCCCCGCGAC AAGCGAGGAC GAGGAACGCC ACAGTGCCAG CGAGTGTCCC 
GAGGGGGGCT CAGAATCGGA CAGCTCCCCA GACGGACCAG GAAGAGGCCC CCAGGGTACT 
CGGGGCCGGG GCAGCGGAGC ACCTGGTAAC CTGGCCTCTA CCCGAGGCCT CCAGGGACGC 
TCCATGTCTG TCCCTGACGA CGCCCACTTC AGCATGATGG TTTTCAGGAT TGGCATCCCT 
GACTTGCACC AGACGAAATG CCTACGCTTC AACCCGGATG CTACCATCTG GACAGCCAAA 
CAGCAAGTAC TCTGTGCCCT GAGCGAGAGT CTACAGGATG TGCTAAACTA TGGACTGTTC 
CAGCCAGCCA CCTCAGGCCG GGATGCCAAC TTCCTGGAGG AAGAGAGGCT TCTAAGAGAG 
TACCCTCAGT CCTTTGAGAA AGGGGTACCC TACCTGGAGT TCCGCTACAA GACCCGAGTC 
TACAAACAGA CCAACCTGGA TGAGAAACAG CTGGCCAAAC TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TTGAATATGT GCAGCTCGGG ACGTCTGACA AGGTGGCGAG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA CCACGACTCA GATTCAGGAG AGACACCTTT GACCCTGGCG 
GCCCAGACTG AAGGCTCAGT AGAGGTGATT CGGACGCTGT GCCTGGGCGG GGCGCACATA 
GACTTCAGGG CCCGGGATGG CATGACAGCG CTGCACAAAG CTGCATGTGC TAGGCACTGC 
CTGGCACTCA CGGCACTTCT GGACCTTGGG GGCTCCCCCA ACTACAAGGA TCGACGAGGG 
CTGACCCCCC TGTTCCACAC AGCTATGGTT GGGGGAGACC CCCGCTGTTG TGAGCTACTT 
CTGTACAACA GGGCCCAGCT GGGCATTGCA GATGAGAACG GCTGGCAGGA AATCCACCAG 
GCCTGCCAAC GAGGGCACTC TCAGCACCTG GAACACCTGC TCTTCTATGG GGCTGAGCCT 
GGAGCCCAGA ATGCCTCAGG AAACACGGCC CTGCACATCT GTGCTCTCTA CAACAAGGAG 
ACCTGTGCCA GGATCCTCCT TTATCGCGGA GCAAACAAGG ATGTGAAAAA TAACAACGGA 
CAGACACCCT TCCAGGTGGC TGTGATTGCT GGGAATTTTG AGCTGGGGGA ACTGATTCGT 
AACCATCGGG AACAGGATGT GGTACCCTTC CAGGAGTCCC CCAAGTATGC AGCCCGGCGT 
CGGGGACCCC CAGGCGCAGG GCTGACTGTA CCCCCAGCGC TGCTGCGGGC TAACAGCGAC 
ACGAGCATGG CGCTGCCTGA TTGGATGGTG TTTTCGGCCC CAGGGGCCTC ATCCTCTGGG 
ACCCCTGGCC CTACCTCAGG ACCCCAGGGC CAGTCACAGC CCTCGGCCCC CAGCACCAAG 
CTCAGTAGTG GGACCCTCAG AAGTGCCAGC AGCCCCCGAG GTGCCCGGGC ACGCTCCCCA 
TCCCGGGGGA GACATCCTGA AGATGCCAAG AGACAACCTC GAGGCCGACC CAGCTCCAGC 
GGGACACCCC GAGATGGGCC GGCTGGGGGA ACTGGGGGCT CAGGGGGCCC TGGGGGCTCC 
CTGGGTAGCA GAGGCAGACG TAGGAAGCTC TATTCAGCGG TACCTGGACG CTCCTTCATG 
GCGGTGAAGT CATACCAGGC GCAAGGCGAG GGGGAGATCT CTCTGAGTAA AGGCGAAAAG 
ATCAAAGTGC TCAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGAGTT 
GGCTGGTTCC CTTCTGACTG CCTAGAAGAG GTGGCAAACC GTTCCCAGGA GGGAAGACAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCAGGCATT ACACAGTGGG CTCTTACGAC 
AGCTTCGATG CCCCAAGTGA TTACATCATC AAGGAGAAGA CAGTCTTACT GCAGAAGAAG 
GACAGTGAGG GGTTCGGGTT CGTGCTCCGG GGGGCCAAGG CGCAGACCCC CATCGAGGAA 
TTCACCCCCA CCCCGGCCTT CCCGGCGCTG CAGTACCTCG AGTCTGTGGA CGAGGGTGGC 
GTGGCATGGC GGGCTGGACT ACGGATGGGA GACTTCCTCA TCGAGGTGAA TGGACAGAAT 
GTAGTGAAGG TTGGCCACCG CCAGGTGGTC AACATGATCC GACAGGGAGG CAACACACTG 
ATGGTCAAGG TGGTGATGGT GACCAGACAC CCAGACATGG ACGAGGCTGT CCACAAGAAA 
GCATCCCAGC AGGCTAAGCG GCTCCCGCCC CCAGCCATCT CCCTGCGTTC CAAGTCTATG 
ACCTCAGAGC TGGAGGAAAT GGTCTCCCCC TGGAAGAAGA AAATTGAATA CGAGCAACAG 
CCCGCGGCAG TGCCCAGCAT GGAGAAAAAG AGGACTGTGT ATCAGATGGC ACTCAACAAA 
CTGGATGAGA TCTTGGCCGC TGCTCAGCAG ACCATCAGCG CAAGTGAGAG TCCTGGGCCT 
GGTGGCCTGG CATCCCTGGG CAAGCACCGA CCCAAAGGAT TCTTTGCCAC TGAGTCGAGC 
TTTGATCCTC ACCACCGCTC CCAGCCAAGT TATGACCGGC CTTCTTTCCT GCCTCCTGGA 
CCCGGCCTTA TGCTCCGGCA GAAATCTATC GGGGCTGCGG AAGACGACAG ACCCTACCTA 
GCACCCCCAG CCATGAAATT CAGTCGTAGC CTGTCAGTCC CCGGTTCAGA GGACATACCC 
CCACCACCCA CTACATCACC ACCAGAGCCG CCCTATAGCA CACCTCCGGC CCCCTCCTCC 
TCCGGCCGCC TCACTCCCTC GCCTCGGGGA GGGCCCTTTA ACCCCAGTTC TGGGGGTCCC 
CTTCCCGCCT CCTCCCCTTC ATCTTTCGAT GGGCCATCTC CTCCTGACAC CCGTGGTGGG 
GGCCGAGAAA AGAGCCTATA TCACAGCGCA GCCCTGCCCC CTGCCCACCA CCATCCACCT 
CATCACCACC ATCATCACGC GCCACCTCCC CAGCCCCACC ATCACCATGC ACATCCTCCT 
CACCCACCGG AGATGGAGAC AGGAGGTTCC CCCGACGATC CCCCACCTCG ATTGGCCCTG 
GGACCCCAGC CCAGCCTTCG AGGCTGGCGA GGTGGAGGAC CCAGCCCGAC CTCGGGGGCC 
CCATCACCTT CCCACCACAG TAGCAGCGGA GGCAGCAGCG GCCCTACTCA GGCTCCCGCC 
CTGCGCTACT TCCAACTGCC CCCGAGGGCG GCCAGTGCGG CTATGTATGT ACCAGCACGT 
TCCGGCCGCG GTCGCAAGGG CCCACTGGTC AAGCAGACCA AAGTGGAAGG CGAGCCCCAA 
AAGGGCAGCA TCCCTTCCGC GTCCTCTCCA ACATCCCCGG CGTTGCCTCG GTCTGAACCA 
CCTCCGGCCG GGCCGTCGGA GAAAAATTCC ATCCCTATCC CGACCATCAT CATCAAAGCC 
CCATCTACCA GTAGCAGCGG CCGCAGCAGT CAGGGAAGCA GCACAGAGGC TGAGCCCCCC 
ACCCAGCCCG ATGGCGCGGG CGGCGGCGGA TCCTCGCCTA GCCCTGCACC TGCCACGTCC 
CCTGTGCCGC CATCCCCGTC GCCCGTGCCC ACTCCCGCGT CACCCAGCGG CCCGGCCACC 
CTGGATTTCA CTAGCCAGTT TGGAGCAGCC CTAGTAGGCG CGGCGCGGAG GGAGGGAGGC 
TGGCAGAACG AAGCCCGGCG GCGGTCCACG CTGTTCCTGT CCACCGACGC AGGAGATGAG 
GATGGAGGCG ACAGCGGGCT GGGCCCGGGG GGGCCCCCGG GTCCCCGGCT GCGCCATTCC 
AAATCCATTG ACGAAGGCAT GTTCTCCGCA GAGCCGTACC TCCGGCTGGA GTCAGGGGGC 
AGCAGTGGGG GCTACGGGGC CTACGCAGCG GGCAGCCGAG CCTACGGGGG CAGCGGGAGC 
AGCAGCGCCT TCACCAGCTT CCTCCCCCCG CGGCCCCTGG TGCATCCGCT GACCGGCAAA 
GCCTTGGATC CCGCCTCTCC GCTGGGGTTG GCCTTGGCCG CCCGGGAGCG GGCCTTGAAG 
GAGTCCTCGG AGGGTGGGGG CACCCCTCAG CCGCCCCCGA GACCTCCATC ACCACGCTAC 
GACGCCCCAC CGCCTACCCT TCACCACCAC TCGCCCCATT CACCCCATTC GCCACATGCG 
CGTCACGAGC CAGTGTTGCG CCTCTGGGGG GACCCCGCGC GCCGGGAGCT GGGGTACCGG 
GCAGGCCTAG GGAGTCAGGA GAAGGCCCTT ACCGCCAGTC CACCTGCAGC TCGCCGCTCC 
CTACTGCACC GTCTGCCCCC GACGGCTCCC GGGGTCGGGC CGCTCCTGCT GCAGCTGGGG 
CCGGAGCCCC CAACTCCCCA TCCTGGGGTG AGCAAGGCCT GGCGCACAGC AGCCCCGGAG 
GAGCCGGAGC GGCTGCCGCT GCACGTGCGA TTCCTTGAGA ATTGCCAAGC CCGGCCTCCC 
CCAGCTGGCA CGAGAGGGTC CTCTACTGAA GATGGGCCTG GGGTCCCGCC TCCCAGCCCT 
CGCAGAGTCT TGCCAACCTC TCCCACCTCC CCACGAGGCA ACGAGGAGAA CGGCCTTCCG 
CTACTGGTCC TGCCACCGCC AGCCCCTTCG GTGGATGTGG ACGACGGCGA GTTCTTGTTT 
GCGGAGCCGC TGCCTCCACC CCTGGAGTTT TCCAATAGTT TCGAAAAGCC AGAGTCGCCT 
CTCACACCCG GGCCTCCTCA CCCACTACCT GACCCTCCCT CCCCGGCTAC CCCTCTACCC 
GCAGCCCCAC CTCCCGCTGT AGCCGCAGCC CCTCCCACCT TGGACTCCAC AGCATCCAGC 
CTGACCTCTT ACGACAGCGA AGTGGCCACA CTGACCCAGG GGGCTCCCGC GGCTCCTGGG 
GATCCTCCAG CTCCAGGCCC GCCGGCCCCG GCAGCCCCAG CTCCCCCAGC ACCGCAGCCA 
GGCCCTGATC CTCCACCTGG CACAGATTCT GGAATTGAGG AGGTGGACAG TCGGAGTAGC 
AGTGATCACC CGCTGGAGAC CATCAGCAGC GCATCCACAC TCAGCAGCTT GTCCGCCGAA 
GGAGGAGGCA ATACAGGGGG CGTGGCTGGG GGCGGGGCCG GGGTGGCCAG TGGGACGGAG 
CTCCTGGACA CCTACGTTGC CTACCTGGAT GGCCAGGCCT TCGGGGGCAG TGGAACTCCT 
GGCCCACCAT ATCCTCCCCA GCTCATGACT CCTTCTAAGC TCCGGGGCAG GGCGCTTGGG 
ACTAGTGGAA ATTTAAGACC TGGTCCCAGT GGGGGACTCC GAGATCCTGT CACCCCCACC 
AGCCCCACTG TCTCTGTGAC CGGAGCTGGA ACAGATGGGC TACTGGCCTT GAGTGCTTGC 
CCTGGACCCT CAACGGCAGG TGTGGCAGGG GGTCCCGTGG CAGTTGAGCC AGAAGTCCCG 
CCTGTACCTT TGCCCGCTGC CTCATCCCTG CCTCGGAAGC TATTACCGTG GGAGGAGGGT 
CCGGGACCAC CACCACCACC CCTGCCAGGG CCCCTGTCCC AGCCTCAGGC CTCTGCCTTG 
GCCACGGTCA AAGCCAGCAT CATCAGCGAA CTCAGCTCCA AGCTCCAGCA GTTTGGTGGG 
TCCTCTACCG CAGGAGGAGC TCTGCCTTGG GCTCGGGGAG GCAGCGGGGG CAGCACAGAC 
AGCCACCACG GAGGAGCCAG TTACATCCCA GAGAGGACGT CCTCCCTGCA GCGGCAGAGG 
CTCTCTGAAG ACTCCCAGAC CTCTCTCCTC TCCAAACCCA GCAGCAGCAT CTTTCAGAAC 
TGGCCTAAAC CCCCTCTGCC ACCACTCCCC ACTGGGTCTG GGGTCTCCTC TTCAACAGCT 
GCAGCCCCGG GAGCTACATC CCCTTCAGCC TCATCTGCCT CAGCTTCCAC CAGACACCTG 
CAGGGTGTGG AATTTGAGAT GCGGCCTCCG CTGCTCCGCC GGGCACCCAG CCCCTCGCTG 
CTGCCTGCCT CGGATCACAA GGTCAGCCCA GCGCCCAGGC CTTCCTCTTT GCCCATCCTG 
CCTTCAGGAC CCATCTACCC GGGCCTCTTC GACATCCGTA GCTCTCCAAC CGGAGGGGCG 
GGAGGCTCGA CTGACCCCTT CGCTCCCGTC TTTGTGCCAC CACACCCCGG GATATCTGGG 
GGTCTTGGAG GAGCCTTGTC TGGGGCCTCT CGCTCCCTAT CACCCACCCG CCTGCTCTCG 
CTGCCCCCGG ACAAGCCGTT CGGCGCCAAG CCTCTAGGGT TCTGGACCAA GTTTGACGTC 
GCCGATTGGC TAGAGTGGCT GGGCTTGTCG GAGCACCGAG CCCAGTTCCT GGACCACGAG 
ATTGATGGCT CCCATCTGCC TGCCTTGACC AAGGAGGACT ATGTGGACCT GGGTGTAACC 
AGGGTGGGCC ACCGTATGAA CATTGACCGG GCTCTCAAAT TTTTCCTGGA GAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Shank Proteins Differentially Regulate Synaptic Transmission.
eNeuro4(6)(2017 Nov-Dec)
Shi R,Redman P,Ghose D,Liu Y,Ren X,Ding LJ,Liu M,Jones KJ,Xu W

SHANK proteins limit integrin activation by directly interacting with Rap1 and?R-Ras.
Nature cell biology19(4)292-305(2017 Apr)
Lilja J,Zacharchenko T,Georgiadou M,Jacquemet G,De Franceschi N,Peuhu E,Hamidi H,Pouwels J,Martens V,Nia FH,Beifuss M,Boeckers T,Kreienkamp HJ,Barsukov IL,Ivaska J

Differential distribution of Shank and GKAP at the postsynaptic density.
PloS one10(3)e0118750(2015)
Tao-Cheng JH,Yang Y,Reese TS,Dosemeci A

Alternative polyadenylation and differential expression of Shank mRNAs in the synaptic neuropil.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences369(1633)20130137(2014 Jan)
Epstein I,Tushev G,Will TJ,Vlatkovic I,Cajigas IJ,Schuman EM

SHANK1 Deletions in Males with Autism Spectrum Disorder.
American journal of human genetics90(5)879-87(2012 May)
Sato D,Lionel AC,Leblond CS,Prasad A,Pinto D,Walker S,O'Connor I,Russell C,Drmic IE,Hamdan FF,Michaud JL,Endris V,Roeth R,Delorme R,Huguet G,Leboyer M,Rastam M,Gillberg C,Lathrop M,Stavropoulos DJ,Anagnostou E,Weksberg R,Fombonne E,Zwaigenbaum L,Fernandez BA,Roberts W,Rappold GA,Marshall CR,Bourgeron T,Szatmari P,Scherer SW

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Shank1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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