Tpgs1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpgs1
Entrez Gene ID	691093
Full Name	tubulin polyglutamylase complex subunit 1
Synonyms	Gtrgeo22,PGs1
General protein information	Preferred Names tubulin polyglutamylase complex subunit 1 Names tubulin polyglutamylase complex subunit 1 gene trap ROSA b-geo 22
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001109621.1	NP_001103091.1	tubulin polyglutamylase complex subunit 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TPGS1	XP_003642907.1
Rattus norvegicus (Norway rat)	Tpgs1	NP_001103091.1
Canis lupus familiaris (dog)	TPGS1	XP_855240.2
Homo sapiens (human)	TPGS1	NP_277048.2
Bos taurus (cattle)	TPGS1	XP_002689183.2
Mus musculus (house mouse)	Tpgs1	NP_683736.1
Xenopus tropicalis (tropical clawed frog)	tpgs1	XP_002939810.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS
The EMBO journal30(8)1520-35(2011 Apr)

Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function.
Ikegami K, Heier RL, Taruishi M, Takagi H, Mukai M, Shimma S, Taira S, Hatanaka K, Morone N, Yao I, Campbell PK, Yuasa S, Janke C, Macgregor GR, Setou M
Proceedings of the National Academy of Sciences of the United States of America104(9)3213-8(2007 Feb)

Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase.
Regnard C, Fesquet D, Janke C, Boucher D, Desbruy?res E, Koulakoff A, Insina C, Travo P, Edd? B
Journal of cell science116(Pt 20)4181-90(2003 Oct)

Mutation of a novel gene results in abnormal development of spermatid flagella, loss of intermale aggression and reduced body fat in mice.
Campbell PK, Waymire KG, Heier RL, Sharer C, Day DE, Reimann H, Jaje JM, Friedrich GA, Burmeister M, Bartness TJ, Russell LD, Young LJ, Zimmer M, Jenne DE, MacGregor GR
Genetics162(1)307-20(2002 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Isolation and functional characterization of the mouse PGs1 ortholog, and comparison to the rat protein.
Title: Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase.

The following Tpgs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpgs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10548	NM_001109621.1 Latest version!	Rattus norvegicus tubulin polyglutamylase complex subunit 1 (Tpgs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpgs1 ( NM_001109621.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001103091.1 Rattus norvegicus tubulin polyglutamylase complex subunit 1 (Tpgs1), mRNA.

Price & Availability↑

CloneID	ORa10548
Clone ID Related Accession (Same CDS sequence)	NM_001109621.1
Accession Version	NM_001109621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	tubulin polyglutamylase complex subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474029.1. On Oct 4, 2007 this sequence version replaced XM_001076820.1. ##Evidence-Data-START## Transcript exon combination :: FQ212876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGC TTCCGGTCCC GCTGCCCCCA GCAAAGATGG CGGCAGTGGA GAAACGACGG 
CCGGCAGTAG CACCAGCGGC CAATTTCCCA GACAGTGGCC GGACGTCGGT GTCTCAGGCG 
GTGGCGGCGG CTGAGAGCGA GGAGGATTTC CTACGGCAGG TTGGCGTGAC GGAGATGCTG 
CGCGCAGCCC TCCTGAAGGT GCTGGAGACC CGGCCCGAAG AGCCCATCGC CTTCTTGGCG 
CATTACTTCG AGAACATGGG TTTGCGCTCG CCGGCCAACG GAGGCGCCGG GGAGCCCCCG 
GGCCAGCTCC TCCTGCAGCA ACAGCGCCTG GGTCGCGCGC TGTGGCACCT TCGCCTGGCT 
CACCACTCGC AGAGGACGGC CTTCAACAAC AACGTGAGCG TGGCCTATGA GTGCCTGAGT 
GCGAGCGGCC GCAAGAAGAA GCCAGGCCTG GATGGGCGCA CATACAGCGA GCTGCTGAAG 
CGAGTTTGCA GGGACGGCGG GGCGCCTGAG GAAGTGGTGG CACCGCTGCT GCGCAAGATC 
CAGTGCCGAG ACCATGAGGC TGTGCCTCTG GGCATCTTCA GAACCGGCAT GCTGTCCTGC 
TTTGTGCTGC TGGAATTCGT GGCGCGCGCC AGCGCCCTCT TCCAGCTGCT GGAGGACCCT 
GGCCTAGCGG TGGCTGATCG GCGTGTGGGT CAGGCTGTGC TGGACACGCT CGAGGGTGCG 
CTGAGCGCAG GAGACCTGGC AACGGCGCCC ACGCACTACC TGGAGGCCGG TTCCCGCCTG 
GGCCCCGACA ACCTGGCGCG TGCCCTGGAC AGGGCAGCAG CCGGGCGACG ACCCAGTGCA 
CCCATGGCCC GCGAGGAGTT CTTGGAGAAG GCTGCAGCCC TCTTCATTGC CAAGGTCAAG 
CCAGTGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103091.1
CDS	441..1352
Translation	MAALPVPLPPAKMAAVEKRRPAVAPAANFPDSGRTSVSQAVAAAESEEDFLRQVGVTEMLRAALLKVLETRPEEPIAFLAHYFENMGLRSPANGGAGEPPGQLLLQQQRLGRALWHLRLAHHSQRTAFNNNVSVAYECLSASGRKKKPGLDGRTYSELLKRVCRDGGAPEEVVAPLLRKIQCRDHEAVPLGIFRTGMLSCFVLLEFVARASALFQLLEDPGLAVADRRVGQAVLDTLEGALSAGDLATAPTHYLEAGSRLGPDNLARALDRAAAGRRPSAPMAREEFLEKAAALFIAKVKPVG

Target ORF information:

RefSeq Version	NM_001109621.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tubulin polyglutamylase complex subunit 1 (Tpgs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109621.1

ORF Insert Sequence:

ATGGCGGCGC TTCCGGTCCC GCTGCCCCCA GCAAAGATGG CGGCAGTGGA GAAACGACGG 
CCGGCAGTAG CACCAGCGGC CAATTTCCCA GACAGTGGCC GGACGTCGGT GTCTCAGGCG 
GTGGCGGCGG CTGAGAGCGA GGAGGATTTC CTACGGCAGG TTGGCGTGAC GGAGATGCTG 
CGCGCAGCCC TCCTGAAGGT GCTGGAGACC CGGCCCGAAG AGCCCATCGC CTTCTTGGCG 
CATTACTTCG AGAACATGGG TTTGCGCTCG CCGGCCAACG GAGGCGCCGG GGAGCCCCCG 
GGCCAGCTCC TCCTGCAGCA ACAGCGCCTG GGTCGCGCGC TGTGGCACCT TCGCCTGGCT 
CACCACTCGC AGAGGACGGC CTTCAACAAC AACGTGAGCG TGGCCTATGA GTGCCTGAGT 
GCGAGCGGCC GCAAGAAGAA GCCAGGCCTG GATGGGCGCA CATACAGCGA GCTGCTGAAG 
CGAGTTTGCA GGGACGGCGG GGCGCCTGAG GAAGTGGTGG CACCGCTGCT GCGCAAGATC 
CAGTGCCGAG ACCATGAGGC TGTGCCTCTG GGCATCTTCA GAACCGGCAT GCTGTCCTGC 
TTTGTGCTGC TGGAATTCGT GGCGCGCGCC AGCGCCCTCT TCCAGCTGCT GGAGGACCCT 
GGCCTAGCGG TGGCTGATCG GCGTGTGGGT CAGGCTGTGC TGGACACGCT CGAGGGTGCG 
CTGAGCGCAG GAGACCTGGC AACGGCGCCC ACGCACTACC TGGAGGCCGG TTCCCGCCTG 
GGCCCCGACA ACCTGGCGCG TGCCCTGGAC AGGGCAGCAG CCGGGCGACG ACCCAGTGCA 
CCCATGGCCC GCGAGGAGTT CTTGGAGAAG GCTGCAGCCC TCTTCATTGC CAAGGTCAAG 
CCAGTGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
The EMBO journal30(8)1520-35(2011 Apr)
Jakobsen L,Vanselow K,Skogs M,Toyoda Y,Lundberg E,Poser I,Falkenby LG,Bennetzen M,Westendorf J,Nigg EA,Uhlen M,Hyman AA,Andersen JS

Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function.
Proceedings of the National Academy of Sciences of the United States of America104(9)3213-8(2007 Feb)
Ikegami K,Heier RL,Taruishi M,Takagi H,Mukai M,Shimma S,Taira S,Hatanaka K,Morone N,Yao I,Campbell PK,Yuasa S,Janke C,Macgregor GR,Setou M

Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase.
Journal of cell science116(Pt 20)4181-90(2003 Oct)
Regnard C,Fesquet D,Janke C,Boucher D,Desbruy?res E,Koulakoff A,Insina C,Travo P,Edd? B

Mutation of a novel gene results in abnormal development of spermatid flagella, loss of intermale aggression and reduced body fat in mice.
Genetics162(1)307-20(2002 Sep)
Campbell PK,Waymire KG,Heier RL,Sharer C,Day DE,Reimann H,Jaje JM,Friedrich GA,Burmeister M,Bartness TJ,Russell LD,Young LJ,Zimmer M,Jenne DE,MacGregor GR

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Tpgs1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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