Prima1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prima1
Entrez Gene ID	690195
Full Name	proline rich membrane anchor 1
Synonyms	PRiMA,Prima1l
General protein information	Preferred Names proline rich membrane anchor 1 Names proline-rich membrane anchor 1 proline rich membrane anchor 1-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_006240495.3	XP_006240557.1	proline-rich membrane anchor 1 isoform X3
XM_008764849.2	XP_008763071.1	proline-rich membrane anchor 1 isoform X1
NM_001108721.2	NP_001102191.2	proline-rich membrane anchor 1 precursor
XM_017594373.1	XP_017449862.1	proline-rich membrane anchor 1 isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	prima1	XP_002933274.1
Bos taurus (cattle)	PRIMA1	NP_001070955.1
Pan troglodytes (chimpanzee)	PRIMA1	XP_003952611.1
Mus musculus (house mouse)	Prima1	NP_579942.2
Gallus gallus (chicken)	PRIMA1	NP_001138964.1
Homo sapiens (human)	PRIMA1	NP_821092.1
Canis lupus familiaris (dog)	PRIMA1	XP_854468.1
Rattus norvegicus (Norway rat)	Prima1	NP_001102191.2

Gene Ontology
Bibliography

Related articles in PubMed

Targeting acetylcholinesterase to membrane rafts: a function mediated by the proline-rich membrane anchor (PRiMA) in neurons.
Xie HQ, Liang D, Leung KW, Chen VP, Zhu KY, Chan WK, Choi RC, Massouli? J, Tsim KW
The Journal of biological chemistry285(15)11537-46(2010 Apr)

Expression and Localization of PRiMA-linked globular form acetylcholinesterase in vertebrate neuromuscular junctions.
Tsim KW, Leung KW, Mok KW, Chen VP, Zhu KY, Zhu JT, Guo AJ, Bi CW, Zheng KY, Lau DT, Xie HQ, Choi RC
Journal of molecular neuroscience : MN40(1-2)40-6(2010 Jan)

Restricted localization of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase at the neuromuscular junctions--contribution and expression from motor neurons.
Leung KW, Xie HQ, Chen VP, Mok MK, Chu GK, Choi RC, Tsim KW
The FEBS journal276(11)3031-42(2009 Jun)

Transcriptional regulation of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase in neuron: an inductive effect of neuron differentiation.
Xie HQ, Choi RC, Leung KW, Chen VP, Chu GK, Tsim KW
Brain research126513-23(2009 Apr)

Acetylcholinesterase associates differently with its anchoring proteins ColQ and PRiMA.
Noureddine H, Carvalho S, Schmitt C, Massouli? J, Bon S
The Journal of biological chemistry283(30)20722-32(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PRiMA in neurons has a role in targeting acetylcholinesterase to membrane rafts
Title: Targeting acetylcholinesterase to membrane rafts: a function mediated by the proline-rich membrane anchor (PRiMA) in neurons.

These findings suggest that the restricted localization of PRiMA-linked G4 AChE at the nmjs could be contributed by the pre-synaptic motor neuron and/or the post-synaptic muscle fiber.
Title: Expression and Localization of PRiMA-linked globular form acetylcholinesterase in vertebrate neuromuscular junctions.

Data show that after denervation, the expression of PRiMA, AChE(T) and G(4) AChE decreased markedly in the spinal cord, and in fast- and slow-twitch muscles.
Title: Restricted localization of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase at the neuromuscular junctions--contribution and expression from motor neurons.

These results suggested that a MAP kinase signaling pathway served as one of the transcriptional regulators in controlling PRiMA gene expression during the neuronal differentiation process.
Title: Transcriptional regulation of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase in neuron: an inductive effect of neuron differentiation.

Acetylcholinesterase associates differently with its anchoring proteins ColQ and PRiMA
Title: Acetylcholinesterase associates differently with its anchoring proteins ColQ and PRiMA.

The following Prima1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prima1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11830	NM_001108721.2 Latest version!	Rattus norvegicus proline rich membrane anchor 1 (Prima1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa11830	XM_006240495.3 Latest version!	Rattus norvegicus proline rich membrane anchor 1 (Prima1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa50457	XM_008764849.2 Latest version!	Rattus norvegicus proline rich membrane anchor 1 (Prima1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa61766	XM_017594373.1 Latest version!	Rattus norvegicus proline rich membrane anchor 1 (Prima1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prima1 ( NM_001108721.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102191.2 Rattus norvegicus proline rich membrane anchor 1 (Prima1), mRNA.

Price & Availability↑

CloneID	ORa11830
Clone ID Related Accession (Same CDS sequence)	XM_006240495.3 , NM_001108721.2
Accession Version	NM_001108721.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	proline-rich membrane anchor 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133316.3 and AABR07073257.1. On or before Sep 25, 2008 this sequence version replaced XM_001073629.1, XM_001066450.1, NM_001108721.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FM029865.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCTTCTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAT 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACGC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCTCCTCTG 
CCCCCGCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CTGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CCGCGGAGGA CAGCTGGTGG TCTGGTCTGG TAATCATCGT CGCGGTGGTC 
TGTGCCTCTC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAAGATGA AAATGGCGCC AGCGTAGCTG AGTACCCCAT GAGCTCCTCG 
CCGAGTAACA AAGGGGTGGA TGTGAACGCT GCGGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102191.2
CDS	103..564
Translation	MLLRDLVLRHGCCWPSLLLHCALHPLWGLVQVTHAEPQKSCSKVTDSCQHICQCRPPPPLPPPPPPPPPPRLLSAPAPNSTSCPAEDSWWSGLVIIVAVVCASLVFLTVLVIICYKAIKRKPLRKDENGASVAEYPMSSSPSNKGVDVNAAVV

Target ORF information:

RefSeq Version	NM_001108721.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proline rich membrane anchor 1 (Prima1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108721.2

ORF Insert Sequence:

ATGCTTCTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAT 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACGC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCTCCTCTG 
CCCCCGCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CTGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CCGCGGAGGA CAGCTGGTGG TCTGGTCTGG TAATCATCGT CGCGGTGGTC 
TGTGCCTCTC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAAGATGA AAATGGCGCC AGCGTAGCTG AGTACCCCAT GAGCTCCTCG 
CCGAGTAACA AAGGGGTGGA TGTGAACGCT GCGGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prima1 ( XM_006240495.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240557.1 Rattus norvegicus proline rich membrane anchor 1 (Prima1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa11830
Clone ID Related Accession (Same CDS sequence)	XM_006240495.3 , NM_001108721.2
Accession Version	XM_006240495.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	proline-rich membrane anchor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240495.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAT 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACGC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCTCCTCTG 
CCCCCGCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CTGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CCGCGGAGGA CAGCTGGTGG TCTGGTCTGG TAATCATCGT CGCGGTGGTC 
TGTGCCTCTC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAAGATGA AAATGGCGCC AGCGTAGCTG AGTACCCCAT GAGCTCCTCG 
CCGAGTAACA AAGGGGTGGA TGTGAACGCT GCGGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240557.1
CDS	517..978
Translation	MLLRDLVLRHGCCWPSLLLHCALHPLWGLVQVTHAEPQKSCSKVTDSCQHICQCRPPPPLPPPPPPPPPPRLLSAPAPNSTSCPAEDSWWSGLVIIVAVVCASLVFLTVLVIICYKAIKRKPLRKDENGASVAEYPMSSSPSNKGVDVNAAVV

Target ORF information:

RefSeq Version	XM_006240495.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proline rich membrane anchor 1 (Prima1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240495.3

ORF Insert Sequence:

ATGCTTCTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAT 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACGC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCTCCTCTG 
CCCCCGCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CTGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CCGCGGAGGA CAGCTGGTGG TCTGGTCTGG TAATCATCGT CGCGGTGGTC 
TGTGCCTCTC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAAGATGA AAATGGCGCC AGCGTAGCTG AGTACCCCAT GAGCTCCTCG 
CCGAGTAACA AAGGGGTGGA TGTGAACGCT GCGGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prima1 ( XM_008764849.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763071.1 Rattus norvegicus proline rich membrane anchor 1 (Prima1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa50457
Clone ID Related Accession (Same CDS sequence)	XM_008764849.2
Accession Version	XM_008764849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	proline-rich membrane anchor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCTG TCCAGCCAGC CGCTGAGAAC AGAGCTGCCG TGGGGGGAAC CCTGAGCTCC 
CTTCTCCGTG TCGACGATCG GTGGGGGTCG CCACCAGGCA AGTTAGCTCA CCAGTGGGCG 
CCGCGGGCCG AGATGCTTCT CCGGGACCTG GTGCTGCGCC ATGGCTGCTG CTGGCCCTCA 
CTTCTGCTGC ATTGTGCGCT GCACCCACTC TGGGGTTTAG TTCAGGTGAC GCACGCTGAG 
CCCCAGAAGT CTTGCTCTAA GGTGACTGAC AGCTGCCAAC ACATCTGCCA GTGCCGGCCC 
CCACCTCCTC TGCCCCCGCC ACCCCCACCT CCGCCACCTC CCAGACTACT CTCTGCCCCA 
GCTCCCAACT CTACCTCCTG CCCCGCGGAG GACAGCTGGT GGTCTGGTCT GGTAATCATC 
GTCGCGGTGG TCTGTGCCTC TCTGGTGTTT CTGACTGTCC TGGTCATCAT TTGCTACAAA 
GCCATAAAGA GGAAACCACT GAGAAAAGAT GAAAATGGCG CCAGCGTAGC TGAGTACCCC 
ATGAGCTCCT CGCCGAGTAA CAAAGGGGTG GATGTGAACG CTGCGGTGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763071.1
CDS	30..623
Translation	MGAVQPAAENRAAVGGTLSSLLRVDDRWGSPPGKLAHQWAPRAEMLLRDLVLRHGCCWPSLLLHCALHPLWGLVQVTHAEPQKSCSKVTDSCQHICQCRPPPPLPPPPPPPPPPRLLSAPAPNSTSCPAEDSWWSGLVIIVAVVCASLVFLTVLVIICYKAIKRKPLRKDENGASVAEYPMSSSPSNKGVDVNAAVV

Target ORF information:

RefSeq Version	XM_008764849.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proline rich membrane anchor 1 (Prima1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764849.2

ORF Insert Sequence:

ATGGGGGCTG TCCAGCCAGC CGCTGAGAAC AGAGCTGCCG TGGGGGGAAC CCTGAGCTCC 
CTTCTCCGTG TCGACGATCG GTGGGGGTCG CCACCAGGCA AGTTAGCTCA CCAGTGGGCG 
CCGCGGGCCG AGATGCTTCT CCGGGACCTG GTGCTGCGCC ATGGCTGCTG CTGGCCCTCA 
CTTCTGCTGC ATTGTGCGCT GCACCCACTC TGGGGTTTAG TTCAGGTGAC GCACGCTGAG 
CCCCAGAAGT CTTGCTCTAA GGTGACTGAC AGCTGCCAAC ACATCTGCCA GTGCCGGCCC 
CCACCTCCTC TGCCCCCGCC ACCCCCACCT CCGCCACCTC CCAGACTACT CTCTGCCCCA 
GCTCCCAACT CTACCTCCTG CCCCGCGGAG GACAGCTGGT GGTCTGGTCT GGTAATCATC 
GTCGCGGTGG TCTGTGCCTC TCTGGTGTTT CTGACTGTCC TGGTCATCAT TTGCTACAAA 
GCCATAAAGA GGAAACCACT GAGAAAAGAT GAAAATGGCG CCAGCGTAGC TGAGTACCCC 
ATGAGCTCCT CGCCGAGTAA CAAAGGGGTG GATGTGAACG CTGCGGTGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prima1 ( XM_017594373.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449862.1 Rattus norvegicus proline rich membrane anchor 1 (Prima1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa61766
Clone ID Related Accession (Same CDS sequence)	XM_017594373.1
Accession Version	XM_017594373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	proline-rich membrane anchor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCTG TCCAGCCAGC CGCTGAGAAC AGAGCTGCCG TGGGGGGAAC CCTGAGCTCC 
CTTCTCCGTG TCGACGATCG GTGGGGGTCG CCACCAGGCA AGTTAGCTCA CCAGTGGGCG 
CCGCGGGCCG AGATGCTTCT CCGGGACCTG GTGCTGCGCC ATGGCTGCTG CTGGCCCTCA 
CTTCTGCTGC ATTGTGCGCT GCACCCACTC TGGGGTTTAG TTCAGGTGAC GCACGCTGAG 
CCCCAGAAGT CTTGCTCTAA GGTGACTGAC AGCTGCCAAC ACATCTGCCA GTGCCGGCCC 
CCACCTCCTC TGCCCCCGCC ACCCCCACCT CCGCCACCTC CCAGACTACT CTCTGCCCCA 
GCTCCCAACT CTACCTCCTG CCCCGCGGAG GACAGCTGGT GGTCTGGTCT GGTAATCATC 
GTCGCGGTGG TCTGTGCCTC TCTGGTGTTT CTGACTGTCC TGGTCATCAT TTGCTACAAA 
GCCATAAAGA GGAACCAAGC CATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449862.1
CDS	35..541
Translation	MGAVQPAAENRAAVGGTLSSLLRVDDRWGSPPGKLAHQWAPRAEMLLRDLVLRHGCCWPSLLLHCALHPLWGLVQVTHAEPQKSCSKVTDSCQHICQCRPPPPLPPPPPPPPPPRLLSAPAPNSTSCPAEDSWWSGLVIIVAVVCASLVFLTVLVIICYKAIKRNQAI

Target ORF information:

RefSeq Version	XM_017594373.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proline rich membrane anchor 1 (Prima1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594373.1

ORF Insert Sequence:

ATGGGGGCTG TCCAGCCAGC CGCTGAGAAC AGAGCTGCCG TGGGGGGAAC CCTGAGCTCC 
CTTCTCCGTG TCGACGATCG GTGGGGGTCG CCACCAGGCA AGTTAGCTCA CCAGTGGGCG 
CCGCGGGCCG AGATGCTTCT CCGGGACCTG GTGCTGCGCC ATGGCTGCTG CTGGCCCTCA 
CTTCTGCTGC ATTGTGCGCT GCACCCACTC TGGGGTTTAG TTCAGGTGAC GCACGCTGAG 
CCCCAGAAGT CTTGCTCTAA GGTGACTGAC AGCTGCCAAC ACATCTGCCA GTGCCGGCCC 
CCACCTCCTC TGCCCCCGCC ACCCCCACCT CCGCCACCTC CCAGACTACT CTCTGCCCCA 
GCTCCCAACT CTACCTCCTG CCCCGCGGAG GACAGCTGGT GGTCTGGTCT GGTAATCATC 
GTCGCGGTGG TCTGTGCCTC TCTGGTGTTT CTGACTGTCC TGGTCATCAT TTGCTACAAA 
GCCATAAAGA GGAACCAAGC CATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Targeting acetylcholinesterase to membrane rafts: a function mediated by the proline-rich membrane anchor (PRiMA) in neurons.
The Journal of biological chemistry285(15)11537-46(2010 Apr)
Xie HQ,Liang D,Leung KW,Chen VP,Zhu KY,Chan WK,Choi RC,Massouli? J,Tsim KW

Expression and Localization of PRiMA-linked globular form acetylcholinesterase in vertebrate neuromuscular junctions.
Journal of molecular neuroscience : MN40(1-2)40-6(2010 Jan)
Tsim KW,Leung KW,Mok KW,Chen VP,Zhu KY,Zhu JT,Guo AJ,Bi CW,Zheng KY,Lau DT,Xie HQ,Choi RC

Restricted localization of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase at the neuromuscular junctions--contribution and expression from motor neurons.
The FEBS journal276(11)3031-42(2009 Jun)
Leung KW,Xie HQ,Chen VP,Mok MK,Chu GK,Choi RC,Tsim KW

Transcriptional regulation of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase in neuron: an inductive effect of neuron differentiation.
Brain research126513-23(2009 Apr)
Xie HQ,Choi RC,Leung KW,Chen VP,Chu GK,Tsim KW

Acetylcholinesterase associates differently with its anchoring proteins ColQ and PRiMA.
The Journal of biological chemistry283(30)20722-32(2008 Jul)
Noureddine H,Carvalho S,Schmitt C,Massouli? J,Bon S

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Prima1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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