gRNAs
The following Pcca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa04437 | |
| Clone ID Related Accession (Same CDS sequence) | NM_019330.1 | |
| Accession Version | NM_019330.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-31 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | propionyl-CoA carboxylase alpha chain, mitochondrial | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CK228512.1, M22631.1, CK601565.1, CO574538.1, CX570803.1 and BQ191052.1. On Oct 17, 2007 this sequence version replaced XM_001075496.1. Sequence Note: The sequence M22631.1 is from a chimeric clone. Only the propionyl-coenzyme A carboxylase sequence region was propagated into this RefSeq record. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCGGGGC TGTGGGTCAG GACAGTGGCA CTATTGGCGG CCAGGCGGCA TTGGCGGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_062203.1 |
| CDS | 33..2204 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_019330.1 |
1 | ATGGCGGGGC TGTGGGTCAG GACAGTGGCA CTATTGGCGG CCAGGCGGCA TTGGCGGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa58649 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017599802.1 | |
| Accession Version | XM_017599802.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | propionyl-CoA carboxylase alpha chain, mitochondrial isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 25% of CDS bases ##RefSeq-Attributes-END## | |
1 | ATGGCGGGGC TGTGGGTCAG GACAGTGGCA CTATTGGCGG CCAGGCGGCA TTGGCGGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017455291.1 |
| CDS | 54..2840 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017599802.1 |
1 | ATGGCGGGGC TGTGGGTCAG GACAGTGGCA CTATTGGCGG CCAGGCGGCA TTGGCGGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Evidence for catabolic pathway of propionate metabolism in CNS: expression pattern of methylmalonyl-CoA mutase and propionyl-CoA carboxylase alpha-subunit in developing and adult rat brain. |
Identification of candidate disease genes by EST alignments, synteny, and expression and verification of Ensembl genes on rat chromosome 1q43-54. |
High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence. |
Three novel splice mutations in the PCCA gene causing identical exon skipping in propionic acidemia patients. |
Sequence analysis, biogenesis, and mitochondrial import of the alpha-subunit of rat liver propionyl-CoA carboxylase. |
