Tlr8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tlr8
Entrez Gene ID	684440
Full Name	toll-like receptor 8
General protein information	Preferred Names toll-like receptor 8 Names toll-like receptor 8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001101009.1	NP_001094479.1	toll-like receptor 8 precursor

Pathways from BioSystems

Homologies

	TLR8	NP_001123899.1
Mus musculus (house mouse)	Tlr8	NP_573475.2
Xenopus tropicalis (tropical clawed frog)	LOC100495962	XP_004912402.1
Homo sapiens (human)	TLR8	NP_619542.1
Rattus norvegicus (Norway rat)	Tlr8	NP_001094479.1
Bos taurus (cattle)	TLR8	NP_001029109.1
Danio rerio (zebrafish)	LOC100332583	XP_002665954.3
Xenopus tropicalis (tropical clawed frog)	tlr8	XP_002933859.1
Pan troglodytes (chimpanzee)	TLR8	NP_001123944.1
Canis lupus familiaris (dog)	TLR8	XP_005641176.1
Danio rerio (zebrafish)	tlr8a	XP_001920594.3

Gene Ontology
Bibliography

Related articles in PubMed

Toll-like receptor 8 senses degradation products of single-stranded RNA.
Tanji H, Ohto U, Shibata T, Taoka M, Yamauchi Y, Isobe T, Miyake K, Shimizu T
Nature structural & molecular biology22(2)109-15(2015 Feb)

CD4 ligation on human blood monocytes triggers macrophage differentiation and enhances HIV infection.
Zhen A, Krutzik SR, Levin BR, Kasparian S, Zack JA, Kitchen SG
Journal of virology88(17)9934-46(2014 Sep)

Regulation of angiogenesis, mural cell recruitment and adventitial macrophage behavior by Toll-like receptors.
Aplin AC, Ligresti G, Fogel E, Zorzi P, Smith K, Nicosia RF
Angiogenesis17(1)147-61(2014 Jan)

Myeloid-specific Rictor deletion induces M1 macrophage polarization and potentiates in vivo pro-inflammatory response to lipopolysaccharide.
Festuccia WT, Pouliot P, Bakan I, Sabatini DM, Laplante M
PloS one9(4)e95432(2014)

Structural reorganization of the Toll-like receptor 8 dimer induced by agonistic ligands.
Tanji H, Ohto U, Shibata T, Miyake K, Shimizu T
Science (New York, N.Y.)339(6126)1426-9(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

aortic angiogenesis is preceded by an immune reaction with overexpression of Toll-like receptors (TLRs) and TLR-inducible genes.
Title: Regulation of angiogenesis, mural cell recruitment and adventitial macrophage behavior by Toll-like receptors.

A five-amino-acid motif in the undefined region of the TLR8 ectodomain is required for species-specific ligand recognition.
Title: A five-amino-acid motif in the undefined region of the TLR8 ectodomain is required for species-specific ligand recognition.

toll-like receptors and nucleotide-binding oligomerization domain 2 combinations are contributors to the signaling cascades resulting in purinergic microglial activation
Title: Combinations of TLR and NOD2 ligands stimulate rat microglial P2X4R expression.

The following Tlr8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tlr8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00527	NM_001101009.1 Latest version!	Rattus norvegicus toll-like receptor 8 (Tlr8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tlr8 ( NM_001101009.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094479.1 Rattus norvegicus toll-like receptor 8 (Tlr8), mRNA.

Price & Availability↑

CloneID	ORa00527
Clone ID Related Accession (Same CDS sequence)	NM_001101009.1
Accession Version	NM_001101009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	toll-like receptor 8 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF032638.1. On Aug 13, 2007 this sequence version replaced XM_001070405.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF032638.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGTCCCCTC AGTCGTGGAT TCTGACCTGC TTTTGCCTGC TCTCCTCTGG AACCAGTGCA 
GTCTTCCTCA AAGGGAACTT TTCCAGAAGC TATCCCTGTG ATGAGAAAAG GCACAATGCC 
CTTGTGACTG CAGAATGCAA TCATCGTCAA CTGCATGAAG TTCCCCAAAC TATCGGCAAA 
TATGTGACAG ATGTAGACTT GTCAGACAAT ACTATTATGC ACATAACAAA TGAGTCCTTT 
CAGAAGTTTC GAAACCTCAC TAAAATCAAT CTGAACCACA ATGCCAAACA ACAGCACCCA 
AATGAAAATA AAAATGGTAT GAATATTACA GAAGGGGCTT TCCTCAGCCT AAGAAATCTA 
ACTGAATTAC TGTTGGAAGA CAACCAGTTA TATACTATAC CTGCTGGGTT GCCTGAGTCT 
TTGAAAGAAC TTAGCCTAAT TCAAAACAAT ATATTTCAGG TAACTAAAAA CAACACTTTC 
GGGCTCAGGA ACTTGGAAAG ACTCTATTTG GGCTGGAACT GCTATTTTAA GTGTAATCAA 
ATCTTTAAAG TAGAAGATGG GGCATTTAAC AATCTTATAA ACTTGAAGCT TCTCTCATTA 
TCTTTCAATA ACCTTTTCTC CGTGCCTCCC AAACTACCAA GCTCTCTAAG CAAACTTTTT 
CTGAGCAATG CCAAGATCTC AACCATCACT CAGGAAGACT TCAAAGGACT GGAACATTTA 
ATATTACTAG ATTTGAGTGG AAACTGCCCA AGGTGTTTCA ATGCCCCATT TCCTTGTGAA 
TCTTGCAACT TAAGTGCGTC CATCAGAATA CATCCTCTTG CTTTTCAAAA TCTCACTCAA 
CTTCGCTTTC TTAACCTCTC TAGCACTTCC CTCAGGACGA TTCCTTCTAC CTGGTTCGAC 
AATCTGACAA ATTTGAAGGA ACTCCATCTT GAATTCAATT ATTTAGTTCA AGAAATTGCC 
TCAGGAGCAT TTTTAACCAA GCTACCCAGT TTACAAACCC TTGACCTGTC CTTCAACTTT 
ATACATAAAG AATATTTACA ATATATTACT ATCTCCCCGA ACTTCTCTAT GCTTCGTTCT 
CTCAGGAAGT TGCACTTAAA AGGTTATGTG TTCCGAGAAC TTAAAAAGGA ACATTTCAAG 
CCCCTCCAGA ATCTTCCAAA CTTAACAACC ATTAACTTGG GCATTAACTT TATTGAGAAA 
ATTGATTTCA AAGCTTTCCA GGATTTTCCC AACCTCAAAG TTATTTACTT ATCGGGAAAT 
CGCATAGCAT CCGTAATAGA TGGTACAGAC CACTCCTCTT GGCGAAACCG TCTTAGGAAG 
CCTCTCTCAA CAGACTATGA CGAGTTTGAT CCACACATGA ATTTTTACCA TAGCACTGAA 
CCTTTAATAA AGCCACAGTG TACTACTTAT GGCAAGGCCT TGGATTTAAG TTTGAACAAT 
ATTTTCGTTA TTGGGAAAAG TCAATTTGAA GGTTTTCAGG ATATCGCCTG CTTAAATCTG 
TCTTTCAATG CCAATGGTCA AGTGTTGAAT GGCACAGAAT TCTCATCCAT GCCCCACATT 
AAATACTTGG ATTTAACCAA CAACAGACTA GACTTTGATG ATAACCAGAC CTTCAGTGAC 
CTTCATGATC TAGAAGTGTT GGACCTGAGC CACAATGCAC ACTATTTCAG TATAGCAGGG 
GTAACACACC GTCTAGGATT TATCCAGAAC TTAATAAAGC TCAAGGTGTT AAACCTGAGC 
CACAATGGCA TTTACACGCT CACAGATGAA TACAAGCTGC AAAGCAAATC TCTGAAAGAG 
TTGGTTTTTA GTGGAAATCG CCTTGACCGT CTGTGGAATG CAAATGATGG CAAATACTGG 
TCCATTTTTA CAAGTCTCGA GACTCTGACA CGCTTGGACT TATCGTACAA TAACCTTCAA 
CAAATCCCAA ATGAAGCATT CCTCAACTTG CCTCAGAGCC TCCAAGAGCT ACATATCAAT 
GATAACAGAT TACGTTTCTT TAATTGGACA TTACTCCAGT ATTTTCCTCA CCTTCATGTG 
CTGGACTTGG GGAGAAATGA GCTGTATTTT CTAACCAATT GCCTATCGAA GTTTACACAT 
TCCCTGAAGA CACTGCTGCT GAACCACAAT CATTTCTCTC ACCTACCTGC TGGCTTCCTC 
TCCGAAGCCA GGAATCTGGT GTACCTGGAT CTAAGTTTCA ACACAATAAA GATGATCAAT 
AAATCCTCCC TGCAAACTGA GACAAAAACA AACTTGTCTG TTCTGGATCT ACAAGGGAAC 
CATTTTGACT GCACGTGTGA CATAAGTGAT TTCCGAAGCT GGCTAGAAGA AAATCCGCAT 
GTCAGAATTC CTAGATTGGT AGATGTCATT TGTTCCAATC CTGGGGATCA GAGATGGAAG 
AGTGTCATGA GCCTAGATCT CACGACTTGT GTGTCAGATA CCACTGCAGC TATCCTGTTC 
TTCTTCACAT TCCTTACCAC CTCCACAGTG CTGTTGGCTG CTCTGGTTCA TCATCTGTTT 
TACTGGGATG TTTGGTTTAT CTATCACATG TGCTCTGCTA AGTTAAGAGG CTACAGGTCT 
TCATCCACAT CCCAAACTTT CTATGATGCT TACATTTCTT ATGACACCAA AGACGCATCC 
GTCACTGACT GGGTGATCAA TGAGCTGCGC TACCACCTTG AAGAGAGTGA GGACAAAAGT 
GTCCTCCTTT GCTTAGAGGA GAGGGATTGG GATCCGGGAT TACCTATCAT TGATAACCTC 
ATGCAGAGCA TAAACCAGAG CAAGAAGACA ATCTTTGTTT TAACCAAGAA ATATGCCAAG 
AGCTGGAACT TTAAAACCGC TTTCTACTTG GCCTTGCAGA GGCTAATGGA TGAGAACATG 
GATGTGATTA TCTTCATCCT CCTGGAACCA GTGTTACAGT ATTCACAGTA CCTGAGGCTT 
CGGCAGAGGA TCTGTAAGAG CTCCATCCTC CAGTGGCCCA ACAACCCCAA AGCGGAAAAC 
TTGTTTTGGC AAAGTCTTAA AAATGTGGTC TTGACCGAAA ATGATTCACG GTATGACAAT 
TTGTACATCG ATTCCATTAG GCAATACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094479.1
CDS	142..3231
Translation	MSPQSWILTCFCLLSSGTSAVFLKGNFSRSYPCDEKRHNALVTAECNHRQLHEVPQTIGKYVTDVDLSDNTIMHITNESFQKFRNLTKINLNHNAKQQHPNENKNGMNITEGAFLSLRNLTELLLEDNQLYTIPAGLPESLKELSLIQNNIFQVTKNNTFGLRNLERLYLGWNCYFKCNQIFKVEDGAFNNLINLKLLSLSFNNLFSVPPKLPSSLSKLFLSNAKISTITQEDFKGLEHLILLDLSGNCPRCFNAPFPCESCNLSASIRIHPLAFQNLTQLRFLNLSSTSLRTIPSTWFDNLTNLKELHLEFNYLVQEIASGAFLTKLPSLQTLDLSFNFIHKEYLQYITISPNFSMLRSLRKLHLKGYVFRELKKEHFKPLQNLPNLTTINLGINFIEKIDFKAFQDFPNLKVIYLSGNRIASVIDGTDHSSWRNRLRKPLSTDYDEFDPHMNFYHSTEPLIKPQCTTYGKALDLSLNNIFVIGKSQFEGFQDIACLNLSFNANGQVLNGTEFSSMPHIKYLDLTNNRLDFDDNQTFSDLHDLEVLDLSHNAHYFSIAGVTHRLGFIQNLIKLKVLNLSHNGIYTLTDEYKLQSKSLKELVFSGNRLDRLWNANDGKYWSIFTSLETLTRLDLSYNNLQQIPNEAFLNLPQSLQELHINDNRLRFFNWTLLQYFPHLHVLDLGRNELYFLTNCLSKFTHSLKTLLLNHNHFSHLPAGFLSEARNLVYLDLSFNTIKMINKSSLQTETKTNLSVLDLQGNHFDCTCDISDFRSWLEENPHVRIPRLVDVICSNPGDQRWKSVMSLDLTTCVSDTTAAILFFFTFLTTSTVLLAALVHHLFYWDVWFIYHMCSAKLRGYRSSSTSQTFYDAYISYDTKDASVTDWVINELRYHLEESEDKSVLLCLEERDWDPGLPIIDNLMQSINQSKKTIFVLTKKYAKSWNFKTAFYLALQRLMDENMDVIIFILLEPVLQYSQYLRLRQRICKSSILQWPNNPKAENLFWQSLKNVVLTENDSRYDNLYIDSIRQY

Target ORF information:

RefSeq Version	NM_001101009.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus toll-like receptor 8 (Tlr8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101009.1

ORF Insert Sequence:

ATGTCCCCTC AGTCGTGGAT TCTGACCTGC TTTTGCCTGC TCTCCTCTGG AACCAGTGCA 
GTCTTCCTCA AAGGGAACTT TTCCAGAAGC TATCCCTGTG ATGAGAAAAG GCACAATGCC 
CTTGTGACTG CAGAATGCAA TCATCGTCAA CTGCATGAAG TTCCCCAAAC TATCGGCAAA 
TATGTGACAG ATGTAGACTT GTCAGACAAT ACTATTATGC ACATAACAAA TGAGTCCTTT 
CAGAAGTTTC GAAACCTCAC TAAAATCAAT CTGAACCACA ATGCCAAACA ACAGCACCCA 
AATGAAAATA AAAATGGTAT GAATATTACA GAAGGGGCTT TCCTCAGCCT AAGAAATCTA 
ACTGAATTAC TGTTGGAAGA CAACCAGTTA TATACTATAC CTGCTGGGTT GCCTGAGTCT 
TTGAAAGAAC TTAGCCTAAT TCAAAACAAT ATATTTCAGG TAACTAAAAA CAACACTTTC 
GGGCTCAGGA ACTTGGAAAG ACTCTATTTG GGCTGGAACT GCTATTTTAA GTGTAATCAA 
ATCTTTAAAG TAGAAGATGG GGCATTTAAC AATCTTATAA ACTTGAAGCT TCTCTCATTA 
TCTTTCAATA ACCTTTTCTC CGTGCCTCCC AAACTACCAA GCTCTCTAAG CAAACTTTTT 
CTGAGCAATG CCAAGATCTC AACCATCACT CAGGAAGACT TCAAAGGACT GGAACATTTA 
ATATTACTAG ATTTGAGTGG AAACTGCCCA AGGTGTTTCA ATGCCCCATT TCCTTGTGAA 
TCTTGCAACT TAAGTGCGTC CATCAGAATA CATCCTCTTG CTTTTCAAAA TCTCACTCAA 
CTTCGCTTTC TTAACCTCTC TAGCACTTCC CTCAGGACGA TTCCTTCTAC CTGGTTCGAC 
AATCTGACAA ATTTGAAGGA ACTCCATCTT GAATTCAATT ATTTAGTTCA AGAAATTGCC 
TCAGGAGCAT TTTTAACCAA GCTACCCAGT TTACAAACCC TTGACCTGTC CTTCAACTTT 
ATACATAAAG AATATTTACA ATATATTACT ATCTCCCCGA ACTTCTCTAT GCTTCGTTCT 
CTCAGGAAGT TGCACTTAAA AGGTTATGTG TTCCGAGAAC TTAAAAAGGA ACATTTCAAG 
CCCCTCCAGA ATCTTCCAAA CTTAACAACC ATTAACTTGG GCATTAACTT TATTGAGAAA 
ATTGATTTCA AAGCTTTCCA GGATTTTCCC AACCTCAAAG TTATTTACTT ATCGGGAAAT 
CGCATAGCAT CCGTAATAGA TGGTACAGAC CACTCCTCTT GGCGAAACCG TCTTAGGAAG 
CCTCTCTCAA CAGACTATGA CGAGTTTGAT CCACACATGA ATTTTTACCA TAGCACTGAA 
CCTTTAATAA AGCCACAGTG TACTACTTAT GGCAAGGCCT TGGATTTAAG TTTGAACAAT 
ATTTTCGTTA TTGGGAAAAG TCAATTTGAA GGTTTTCAGG ATATCGCCTG CTTAAATCTG 
TCTTTCAATG CCAATGGTCA AGTGTTGAAT GGCACAGAAT TCTCATCCAT GCCCCACATT 
AAATACTTGG ATTTAACCAA CAACAGACTA GACTTTGATG ATAACCAGAC CTTCAGTGAC 
CTTCATGATC TAGAAGTGTT GGACCTGAGC CACAATGCAC ACTATTTCAG TATAGCAGGG 
GTAACACACC GTCTAGGATT TATCCAGAAC TTAATAAAGC TCAAGGTGTT AAACCTGAGC 
CACAATGGCA TTTACACGCT CACAGATGAA TACAAGCTGC AAAGCAAATC TCTGAAAGAG 
TTGGTTTTTA GTGGAAATCG CCTTGACCGT CTGTGGAATG CAAATGATGG CAAATACTGG 
TCCATTTTTA CAAGTCTCGA GACTCTGACA CGCTTGGACT TATCGTACAA TAACCTTCAA 
CAAATCCCAA ATGAAGCATT CCTCAACTTG CCTCAGAGCC TCCAAGAGCT ACATATCAAT 
GATAACAGAT TACGTTTCTT TAATTGGACA TTACTCCAGT ATTTTCCTCA CCTTCATGTG 
CTGGACTTGG GGAGAAATGA GCTGTATTTT CTAACCAATT GCCTATCGAA GTTTACACAT 
TCCCTGAAGA CACTGCTGCT GAACCACAAT CATTTCTCTC ACCTACCTGC TGGCTTCCTC 
TCCGAAGCCA GGAATCTGGT GTACCTGGAT CTAAGTTTCA ACACAATAAA GATGATCAAT 
AAATCCTCCC TGCAAACTGA GACAAAAACA AACTTGTCTG TTCTGGATCT ACAAGGGAAC 
CATTTTGACT GCACGTGTGA CATAAGTGAT TTCCGAAGCT GGCTAGAAGA AAATCCGCAT 
GTCAGAATTC CTAGATTGGT AGATGTCATT TGTTCCAATC CTGGGGATCA GAGATGGAAG 
AGTGTCATGA GCCTAGATCT CACGACTTGT GTGTCAGATA CCACTGCAGC TATCCTGTTC 
TTCTTCACAT TCCTTACCAC CTCCACAGTG CTGTTGGCTG CTCTGGTTCA TCATCTGTTT 
TACTGGGATG TTTGGTTTAT CTATCACATG TGCTCTGCTA AGTTAAGAGG CTACAGGTCT 
TCATCCACAT CCCAAACTTT CTATGATGCT TACATTTCTT ATGACACCAA AGACGCATCC 
GTCACTGACT GGGTGATCAA TGAGCTGCGC TACCACCTTG AAGAGAGTGA GGACAAAAGT 
GTCCTCCTTT GCTTAGAGGA GAGGGATTGG GATCCGGGAT TACCTATCAT TGATAACCTC 
ATGCAGAGCA TAAACCAGAG CAAGAAGACA ATCTTTGTTT TAACCAAGAA ATATGCCAAG 
AGCTGGAACT TTAAAACCGC TTTCTACTTG GCCTTGCAGA GGCTAATGGA TGAGAACATG 
GATGTGATTA TCTTCATCCT CCTGGAACCA GTGTTACAGT ATTCACAGTA CCTGAGGCTT 
CGGCAGAGGA TCTGTAAGAG CTCCATCCTC CAGTGGCCCA ACAACCCCAA AGCGGAAAAC 
TTGTTTTGGC AAAGTCTTAA AAATGTGGTC TTGACCGAAA ATGATTCACG GTATGACAAT 
TTGTACATCG ATTCCATTAG GCAATACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Toll-like receptor 8 senses degradation products of single-stranded RNA.
Nature structural & molecular biology22(2)109-15(2015 Feb)
Tanji H,Ohto U,Shibata T,Taoka M,Yamauchi Y,Isobe T,Miyake K,Shimizu T

CD4 ligation on human blood monocytes triggers macrophage differentiation and enhances HIV infection.
Journal of virology88(17)9934-46(2014 Sep)
Zhen A,Krutzik SR,Levin BR,Kasparian S,Zack JA,Kitchen SG

Regulation of angiogenesis, mural cell recruitment and adventitial macrophage behavior by Toll-like receptors.
Angiogenesis17(1)147-61(2014 Jan)
Aplin AC,Ligresti G,Fogel E,Zorzi P,Smith K,Nicosia RF

Myeloid-specific Rictor deletion induces M1 macrophage polarization and potentiates in vivo pro-inflammatory response to lipopolysaccharide.
PloS one9(4)e95432(2014)
Festuccia WT,Pouliot P,Bakan I,Sabatini DM,Laplante M

Structural reorganization of the Toll-like receptor 8 dimer induced by agonistic ligands.
Science (New York, N.Y.)339(6126)1426-9(2013 Mar)
Tanji H,Ohto U,Shibata T,Miyake K,Shimizu T

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Tlr8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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