Thbs3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Thbs3
Entrez Gene ID	681309
Full Name	thrombospondin 3
General protein information	Preferred Names thrombospondin 3 Names thrombospondin-3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305458.1	NP_001292387.1	thrombospondin-3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THBS3	XP_001154764.1
Bos taurus (cattle)	THBS3	NP_001095309.1
Danio rerio (zebrafish)	thbs3a	NP_775332.1
	THBS3	XP_001115770.1
Mus musculus (house mouse)	Thbs3	NP_038719.2
Xenopus tropicalis (tropical clawed frog)	thbs3	NP_001011401.1
Homo sapiens (human)	THBS3	NP_009043.1
Canis lupus familiaris (dog)	THBS3	XP_003434941.1
Rattus norvegicus (Norway rat)	LOC681309	XP_003749368.1

Gene Ontology
Bibliography

Related articles in PubMed

Gabapentin receptor alpha2delta-1 is a neuronal thrombospondin receptor responsible for excitatory CNS synaptogenesis.
Eroglu C, Allen NJ, Susman MW, O'Rourke NA, Park CY, Ozkan E, Chakraborty C, Mulinyawe SB, Annis DS, Huberman AD, Green EM, Lawler J, Dolmetsch R, Garcia KC, Smith SJ, Luo ZD, Rosenthal A, Mosher DF, Barres BA
Cell139(2)380-92(2009 Oct)

Skeletal abnormalities in mice lacking extracellular matrix proteins, thrombospondin-1, thrombospondin-3, thrombospondin-5, and type IX collagen.
Posey KL, Hankenson K, Veerisetty AC, Bornstein P, Lawler J, Hecht JT
The American journal of pathology172(6)1664-74(2008 Jun)

Mice with a disruption of the thrombospondin 3 gene differ in geometric and biomechanical properties of bone and have accelerated development of the femoral head.
Hankenson KD, Hormuzdi SG, Meganck JA, Bornstein P
Molecular and cellular biology25(13)5599-606(2005 Jul)

Corneal stromal cells (keratocytes) express thrombospondins 2 and 3 in wound repair phenotype.
Armstrong DJ, Hiscott P, Batterbury M, Kaye S
The international journal of biochemistry & cell biology34(6)588-93(2002 Jun)

Thrombospondin 3 is a developmentally regulated heparin binding protein.
Qabar AN, Lin Z, Wolf FW, O'Shea KS, Lawler J, Dixit VM
The Journal of biological chemistry269(2)1262-9(1994 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Thbs3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Thbs3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa15287	NM_001305458.1 Latest version!	Rattus norvegicus thrombospondin 3 (Thbs3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Thbs3 ( NM_001305458.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001292387.1 Rattus norvegicus thrombospondin 3 (Thbs3), mRNA.

Price & Availability↑

CloneID	ORa15287
Clone ID Related Accession (Same CDS sequence)	NM_001305458.1
Accession Version	NM_001305458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Rattus norvegicus(Norway rat)
Product	thrombospondin-3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC098750.6. On or before Mar 15, 2015 this sequence version replaced XM_008761238.1, XM_003753605.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689601 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAGC CGGAACTTTG GGTTGCCCTG GCTCTCCTCC TTTGCTCTTA CACATGTGGC 
AGTCAGGACC TTCAGGTAAT TGACCTGCTG ACGGTGAGTG AGTCCAGGCA GATGGCAGCT 
GTAGCAGAGA AGGTCCGGAC AGCTTTACTC ACTGCTGGGG ACATCTACCT CTTGTCCACT 
TTCCGCCTCC CTCCAAAGCA AGGTGGTGTC CTCTTTGGCC TCTACTCTCG CCAAGACAAC 
ACACGATGGC TGGAGGCCTC TGTTGTTGGC AAGATCAACA AAGTGCTGGT GCGGTACCAG 
CGGGAAGATG GCAAAGTGCA CGCAGTGAAC CTTCAGCAAG CAGGCTTGGC TGACGGGCGC 
ACGCACACCG CTCTCCTGCG ACTCCGAGGA CCTTCTCGAC CCAGCCCTGG CCTGCAACTG 
TATGTGGACT GTAAACTGGG TGACCAACAC GCTGGCCTCC CAGCCCTAGC GCCCATTCCT 
CCAGCGGAGG TCAGCGGACT GGAGATTAGA ACAGGACAAA AGGCTTACCT GAGGATGCAG 
GGCTTTGTGG AATCGATGAA GATTATTTTG GGTGGATCGA TGGCTCGGGT GGGAGCCCTG 
AGCGAATGTC CGTTCCAAGG AGATGAATCC ATTCACAATG CAGTGACCAG CGCGCTGCAG 
TCCATTCTAG GAGAGCAGAC CAAGGCACTG GTCACTCAGC TCACACTCTT CAACCAGATC 
CTAGTGGAGC TCCGGGATGA CATCCGGGAC CAGGTGAAGG AAATGTCCCT CATCCGGAAC 
ACCATCATGG AGTGTCAGGT GTGCGGTTTC CACGAGCAGC GTTCACACTG CAGCCCCAGC 
CCCTGCTTCA GAGGTGTGGA CTGCATGGAA GTGTATGAGT ACCCAGGCTA CCGCTGTGGG 
CCCTGCCCAC CTGGCCTGCG CGGCAATGGC ACCCACTGTG AAGACATCGA CGAGTGTGCT 
CACGCTGACC CCTGTTTCCT GGGGTCCAGC TGCATCAACA CCATGCCTGG CTTCCACTGC 
GAGGCCTGTC CTCAAGGGTA CAAGGGCACC CGAGTGTCTG GAGTGGGCAT TGACTATGCT 
AGAGCCAGCA AACAGGTCTG CAGTGACATT GACGAATGTA ACGACGGTAA CAATGGAGGC 
TGTGACCCAA ATTCCATCTG CACCAACACT GTGGGGTCTT TCAAATGTGG TCCCTGCCGC 
CTGGGTTTCC TGGGGAACCA GAGCCAGGGC TGCACTCCAG CCAGGACGTG CCACAGCCCC 
GCCCACAGCC CCTGCCACAT CCACGCTCAT TGTCTTTTTG AACGCAACGG TGCAGTGTCA 
TGCCAGTGTA ACGTGGGCTG GGCAGGGAAC GGGAACGTGT GTGGACCTGA CACGGACATA 
GACGGCTATC CAGACCAGGC GCTTCCCTGC ATGGACAACA ACAAACACTG CAAGCAGGAC 
AATTGCCTTT TGACACCCAA CTCTGGGCAA GAAGATGCTG ATAATGATGG TGTCGGGGAC 
CAGTGTGATG ACGACGCTGA TGGGGATGGT ATCAAGAATG TTGAGGACAA CTGCCGACTG 
TTCCCCAACA AGGACCAGCA GAATTCAGAC ACAGATTCAT TTGGTGACGC CTGTGACAAC 
TGTCCCAATG TTCCCAACAA TGACCAGAAG GACACGGATG GCAATGGAGA AGGAGACGCC 
TGTGACAACG ATGTGGACGG GGACGGCATC CCCAATGGCT TGGACAATTG CCCTAAAGTA 
CCCAACCCAC TGCAGACAGA CCGGGATGAA GATGGAGTCG GAGACACTTG CGACAGCTGC 
CCTGAGATGA GCAATCCTAC CCAGACAGAT GCAGACAGTG ACCTGGTAGG GGATGTCTGT 
GACACCAACG AAGACAGCGA TGGAGACGGG CACCAGGACA CCAAGGACAA CTGTCCTCAG 
CTGCCCAACA GCTCTCAGCT GGACTCGGAC AACGACGGGC TTGGAGATGA GTGTGACAGC 
GACGATGACA ATGACGGGGT GCCCGATTAC GTGCCTCCCG GGCCTGATAA CTGTCGCCTG 
GTACCCAATC CTAACCAGAA GGACTCAGAC GGCAACGGTG TTGGTGATGT CTGTGAGGAT 
GACTTTGACA ATGATGCAGT GGTTGACCCT CTGGATGTGT GCCCAGAAAG TGCAGAAGTT 
ACCCTCACGG ACTTCCGGGC CTATCAGACC GTGATTCTGG ATCCGGAGGG TGACGCTCAG 
ATCGATCCAA ACTGGGTAGT GCTCAACCAG GGCATGGAAA TTGTTCAGAC TATGAACAGT 
GACCCAGGTC TTGCAGTTGG ATACACAGCC TTCAATGGTG TAGACTTTGA AGGCACTTTC 
CACGTGAACA CAGTCACTGA TGATGACTAT GCAGGCTTCC TCTTCAGTTA CCAGGACAGT 
GGCCGCTTCT ATGTGGTCAT GTGGAAACAG ACGGAGCAGA CCTACTGGCA GGCCACGCCT 
TTCCGAGCAG TGGCCCAACC AGGGCTGCAG CTCAAGGCAG TGACATCAAT ATCTGGCCCA 
GGTGAGCACC TCCGGAATGC TCTGTGGCAC ACTGGCCACA CCCCTGATCA GGTACGACTG 
CTGTGGACTG ATCCACGGAA TGTGGGTTGG CGAGACAAGA CATCCTATAG ATGGCGGCTG 
CTCCACCGGC CCCAAGTCGG CTACATTCGG GTGAAGCTCT ATGAGGGTCC CCAGCTAGTG 
GCGGACTCTG GGGTGATTAT TGATACGTCC ATGCGAGGGG GGCGTCTTGG TGTATTCTGC 
TTCTCCCAAG AAAACATCAT TTGGTCTAAT CTCCAGTACC GATGCAATGA CACAGTGCCT 
GAGGACTTTG AGCCATTCAG GAGGCAACTG CTCCAGGGAA GGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292387.1
CDS	35..2902
Translation	MEKPELWVALALLLCSYTCGSQDLQVIDLLTVSESRQMAAVAEKVRTALLTAGDIYLLSTFRLPPKQGGVLFGLYSRQDNTRWLEASVVGKINKVLVRYQREDGKVHAVNLQQAGLADGRTHTALLRLRGPSRPSPGLQLYVDCKLGDQHAGLPALAPIPPAEVSGLEIRTGQKAYLRMQGFVESMKIILGGSMARVGALSECPFQGDESIHNAVTSALQSILGEQTKALVTQLTLFNQILVELRDDIRDQVKEMSLIRNTIMECQVCGFHEQRSHCSPSPCFRGVDCMEVYEYPGYRCGPCPPGLRGNGTHCEDIDECAHADPCFLGSSCINTMPGFHCEACPQGYKGTRVSGVGIDYARASKQVCSDIDECNDGNNGGCDPNSICTNTVGSFKCGPCRLGFLGNQSQGCTPARTCHSPAHSPCHIHAHCLFERNGAVSCQCNVGWAGNGNVCGPDTDIDGYPDQALPCMDNNKHCKQDNCLLTPNSGQEDADNDGVGDQCDDDADGDGIKNVEDNCRLFPNKDQQNSDTDSFGDACDNCPNVPNNDQKDTDGNGEGDACDNDVDGDGIPNGLDNCPKVPNPLQTDRDEDGVGDTCDSCPEMSNPTQTDADSDLVGDVCDTNEDSDGDGHQDTKDNCPQLPNSSQLDSDNDGLGDECDSDDDNDGVPDYVPPGPDNCRLVPNPNQKDSDGNGVGDVCEDDFDNDAVVDPLDVCPESAEVTLTDFRAYQTVILDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTVTDDDYAGFLFSYQDSGRFYVVMWKQTEQTYWQATPFRAVAQPGLQLKAVTSISGPGEHLRNALWHTGHTPDQVRLLWTDPRNVGWRDKTSYRWRLLHRPQVGYIRVKLYEGPQLVADSGVIIDTSMRGGRLGVFCFSQENIIWSNLQYRCNDTVPEDFEPFRRQLLQGRV

Target ORF information:

RefSeq Version	NM_001305458.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thrombospondin 3 (Thbs3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305458.1

ORF Insert Sequence:

ATGGAGAAGC CGGAACTTTG GGTTGCCCTG GCTCTCCTCC TTTGCTCTTA CACATGTGGC 
AGTCAGGACC TTCAGGTAAT TGACCTGCTG ACGGTGAGTG AGTCCAGGCA GATGGCAGCT 
GTAGCAGAGA AGGTCCGGAC AGCTTTACTC ACTGCTGGGG ACATCTACCT CTTGTCCACT 
TTCCGCCTCC CTCCAAAGCA AGGTGGTGTC CTCTTTGGCC TCTACTCTCG CCAAGACAAC 
ACACGATGGC TGGAGGCCTC TGTTGTTGGC AAGATCAACA AAGTGCTGGT GCGGTACCAG 
CGGGAAGATG GCAAAGTGCA CGCAGTGAAC CTTCAGCAAG CAGGCTTGGC TGACGGGCGC 
ACGCACACCG CTCTCCTGCG ACTCCGAGGA CCTTCTCGAC CCAGCCCTGG CCTGCAACTG 
TATGTGGACT GTAAACTGGG TGACCAACAC GCTGGCCTCC CAGCCCTAGC GCCCATTCCT 
CCAGCGGAGG TCAGCGGACT GGAGATTAGA ACAGGACAAA AGGCTTACCT GAGGATGCAG 
GGCTTTGTGG AATCGATGAA GATTATTTTG GGTGGATCGA TGGCTCGGGT GGGAGCCCTG 
AGCGAATGTC CGTTCCAAGG AGATGAATCC ATTCACAATG CAGTGACCAG CGCGCTGCAG 
TCCATTCTAG GAGAGCAGAC CAAGGCACTG GTCACTCAGC TCACACTCTT CAACCAGATC 
CTAGTGGAGC TCCGGGATGA CATCCGGGAC CAGGTGAAGG AAATGTCCCT CATCCGGAAC 
ACCATCATGG AGTGTCAGGT GTGCGGTTTC CACGAGCAGC GTTCACACTG CAGCCCCAGC 
CCCTGCTTCA GAGGTGTGGA CTGCATGGAA GTGTATGAGT ACCCAGGCTA CCGCTGTGGG 
CCCTGCCCAC CTGGCCTGCG CGGCAATGGC ACCCACTGTG AAGACATCGA CGAGTGTGCT 
CACGCTGACC CCTGTTTCCT GGGGTCCAGC TGCATCAACA CCATGCCTGG CTTCCACTGC 
GAGGCCTGTC CTCAAGGGTA CAAGGGCACC CGAGTGTCTG GAGTGGGCAT TGACTATGCT 
AGAGCCAGCA AACAGGTCTG CAGTGACATT GACGAATGTA ACGACGGTAA CAATGGAGGC 
TGTGACCCAA ATTCCATCTG CACCAACACT GTGGGGTCTT TCAAATGTGG TCCCTGCCGC 
CTGGGTTTCC TGGGGAACCA GAGCCAGGGC TGCACTCCAG CCAGGACGTG CCACAGCCCC 
GCCCACAGCC CCTGCCACAT CCACGCTCAT TGTCTTTTTG AACGCAACGG TGCAGTGTCA 
TGCCAGTGTA ACGTGGGCTG GGCAGGGAAC GGGAACGTGT GTGGACCTGA CACGGACATA 
GACGGCTATC CAGACCAGGC GCTTCCCTGC ATGGACAACA ACAAACACTG CAAGCAGGAC 
AATTGCCTTT TGACACCCAA CTCTGGGCAA GAAGATGCTG ATAATGATGG TGTCGGGGAC 
CAGTGTGATG ACGACGCTGA TGGGGATGGT ATCAAGAATG TTGAGGACAA CTGCCGACTG 
TTCCCCAACA AGGACCAGCA GAATTCAGAC ACAGATTCAT TTGGTGACGC CTGTGACAAC 
TGTCCCAATG TTCCCAACAA TGACCAGAAG GACACGGATG GCAATGGAGA AGGAGACGCC 
TGTGACAACG ATGTGGACGG GGACGGCATC CCCAATGGCT TGGACAATTG CCCTAAAGTA 
CCCAACCCAC TGCAGACAGA CCGGGATGAA GATGGAGTCG GAGACACTTG CGACAGCTGC 
CCTGAGATGA GCAATCCTAC CCAGACAGAT GCAGACAGTG ACCTGGTAGG GGATGTCTGT 
GACACCAACG AAGACAGCGA TGGAGACGGG CACCAGGACA CCAAGGACAA CTGTCCTCAG 
CTGCCCAACA GCTCTCAGCT GGACTCGGAC AACGACGGGC TTGGAGATGA GTGTGACAGC 
GACGATGACA ATGACGGGGT GCCCGATTAC GTGCCTCCCG GGCCTGATAA CTGTCGCCTG 
GTACCCAATC CTAACCAGAA GGACTCAGAC GGCAACGGTG TTGGTGATGT CTGTGAGGAT 
GACTTTGACA ATGATGCAGT GGTTGACCCT CTGGATGTGT GCCCAGAAAG TGCAGAAGTT 
ACCCTCACGG ACTTCCGGGC CTATCAGACC GTGATTCTGG ATCCGGAGGG TGACGCTCAG 
ATCGATCCAA ACTGGGTAGT GCTCAACCAG GGCATGGAAA TTGTTCAGAC TATGAACAGT 
GACCCAGGTC TTGCAGTTGG ATACACAGCC TTCAATGGTG TAGACTTTGA AGGCACTTTC 
CACGTGAACA CAGTCACTGA TGATGACTAT GCAGGCTTCC TCTTCAGTTA CCAGGACAGT 
GGCCGCTTCT ATGTGGTCAT GTGGAAACAG ACGGAGCAGA CCTACTGGCA GGCCACGCCT 
TTCCGAGCAG TGGCCCAACC AGGGCTGCAG CTCAAGGCAG TGACATCAAT ATCTGGCCCA 
GGTGAGCACC TCCGGAATGC TCTGTGGCAC ACTGGCCACA CCCCTGATCA GGTACGACTG 
CTGTGGACTG ATCCACGGAA TGTGGGTTGG CGAGACAAGA CATCCTATAG ATGGCGGCTG 
CTCCACCGGC CCCAAGTCGG CTACATTCGG GTGAAGCTCT ATGAGGGTCC CCAGCTAGTG 
GCGGACTCTG GGGTGATTAT TGATACGTCC ATGCGAGGGG GGCGTCTTGG TGTATTCTGC 
TTCTCCCAAG AAAACATCAT TTGGTCTAAT CTCCAGTACC GATGCAATGA CACAGTGCCT 
GAGGACTTTG AGCCATTCAG GAGGCAACTG CTCCAGGGAA GGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gabapentin receptor alpha2delta-1 is a neuronal thrombospondin receptor responsible for excitatory CNS synaptogenesis.
Cell139(2)380-92(2009 Oct)
Eroglu C,Allen NJ,Susman MW,O'Rourke NA,Park CY,Ozkan E,Chakraborty C,Mulinyawe SB,Annis DS,Huberman AD,Green EM,Lawler J,Dolmetsch R,Garcia KC,Smith SJ,Luo ZD,Rosenthal A,Mosher DF,Barres BA

Skeletal abnormalities in mice lacking extracellular matrix proteins, thrombospondin-1, thrombospondin-3, thrombospondin-5, and type IX collagen.
The American journal of pathology172(6)1664-74(2008 Jun)
Posey KL,Hankenson K,Veerisetty AC,Bornstein P,Lawler J,Hecht JT

Mice with a disruption of the thrombospondin 3 gene differ in geometric and biomechanical properties of bone and have accelerated development of the femoral head.
Molecular and cellular biology25(13)5599-606(2005 Jul)
Hankenson KD,Hormuzdi SG,Meganck JA,Bornstein P

Corneal stromal cells (keratocytes) express thrombospondins 2 and 3 in wound repair phenotype.
The international journal of biochemistry & cell biology34(6)588-93(2002 Jun)
Armstrong DJ,Hiscott P,Batterbury M,Kaye S

Thrombospondin 3 is a developmentally regulated heparin binding protein.
The Journal of biological chemistry269(2)1262-9(1994 Jan)
Qabar AN,Lin Z,Wolf FW,O'Shea KS,Lawler J,Dixit VM

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Thbs3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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