Trpm7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trpm7
Entrez Gene ID	679906
Full Name	transient receptor potential cation channel, subfamily M, member 7
Synonyms	Chak,LTrpC-7,Ltrpc7,Trp-plik
General protein information	Preferred Names transient receptor potential cation channel, subfamily M, member 7 Names transient receptor potential cation channel subfamily M member 7 long transient receptor potential channel 7 transient receptor potential-phospholipase C-interacting kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q36
Summary	dual function transmembrane protein; functions as both a calcium-permeant ion channel and serine/threonine protein kinase; implicated in the control of cell division [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053705.2	NP_446157.2	transient receptor potential cation channel subfamily M member 7

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRPM7	NP_060142.3
Pan troglodytes (chimpanzee)	TRPM7	XP_510400.2
Gallus gallus (chicken)	TRPM7	NP_001171026.1
Danio rerio (zebrafish)	trpm7	NP_001025232.1
Canis lupus familiaris (dog)	TRPM7	XP_005638461.1
Mus musculus (house mouse)	Trpm7	NP_001157797.1
Rattus norvegicus (Norway rat)	Trpm7	NP_446157.2
Xenopus tropicalis (tropical clawed frog)	trpm7	XP_002939674.2
Bos taurus (cattle)	TRPM7	NP_001193095.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of ROS-TRPM7-ERK1/2 axis in high concentration glucose-mediated proliferation and phenotype switching of rat aortic vascular smooth muscle cells.
Yang M, Fang J, Liu Q, Wang Y, Zhang Z
Biochemical and biophysical research communications494(3-4)526-533(2017 12)

A TRPM7 variant shows altered sensitivity to magnesium that may contribute to the pathogenesis of two Guamanian neurodegenerative disorders.
Hermosura MC, Nayakanti H, Dorovkov MV, Calderon FR, Ryazanov AG, Haymer DS, Garruto RM
Proceedings of the National Academy of Sciences of the United States of America102(32)11510-5(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These observations suggest that reactive oxygen species-TRPM7-ERK1/2 axis plays an important role in hyperglycemia-induced development of the proliferative phenotype in RAoSMC.
Title: Role of ROS-TRPM7-ERK1/2 axis in high concentration glucose-mediated proliferation and phenotype switching of rat aortic vascular smooth muscle cells.

The role of TRPM7 channel in hypoxia-induced cardiac fibrosis
Title: Astragaloside? against cardiac fibrosis by inhibiting TRPM7 channel.

expression of TRPM7 increased significantly in ASMCs from smokeexposed rats and the upregulation of TRPM7 led to augmented cell proliferation and IL-8 release in ASMCs from rats exposed to cigarette smoke.
Title: Upregulation of TRPM7 augments cell proliferation and interleukin-8 release in airway smooth muscle cells of rats exposed to cigarette smoke.

Data demonstrated that ox-LDL significantly increased TRPM7 activity, and induced VSMC proliferation and migration. VSMC proliferation and migration were inhibited by nonspecific TRPM7 blocker 2-APB or synthetic siRNA targeting TRPM7.
Title: TRPM7 channel regulates ox-LDL-induced proliferation and migration of vascular smooth muscle cells via MEK-ERK pathways.

IL-6 inhibits the inward TRPM7 current via the JAK2-STAT3 signaling pathway
Title: Regulation of TRPM7 Function by IL-6 through the JAK2-STAT3 Signaling Pathway.

The following Trpm7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trpm7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10364	NM_053705.2 Latest version!	Rattus norvegicus transient receptor potential cation channel, subfamily M, member 7 (Trpm7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trpm7 ( NM_053705.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446157.2 Rattus norvegicus transient receptor potential cation channel, subfamily M, member 7 (Trpm7), mRNA.

Price & Availability↑

CloneID	ORa10364
Clone ID Related Accession (Same CDS sequence)	NM_053705.2
Accession Version	NM_053705.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Rattus norvegicus(Norway rat)
Product	transient receptor potential cation channel subfamily M member 7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01024912.1, AAHX01024911.1, AAHX01024910.1 and AAHX01024909.1. On or before Sep 7, 2012 this sequence version replaced XM_001056331.4, XM_002726172.1, NM_053705.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCCAGA AATCCTGGAT AGAGAGCACC TTGACCAAGA GGGAGTGTGT GTATATTATA 
CCAAGCTCCA AAGACCCTCA CAGATGTCTT CCAGGATGTC AGATTTGTCA GCAACTTGTC 
AGATGTTTCT GTGGTCGTTT GGTCAAGCAG CATGCATGCT TTACTGCAAG TCTTGCCACA 
AAATACTCAG ATGTGAAATT GGGTGAACAC TTTAACCAGG CAATAGAAGA ATGGTCTGTG 
GAAAAGCACA CGGAGCAGAG CCCAACAGAT GCTTATGGAG TCATCAATTT TCAAGGGGGT 
TCTCATTCCT ACAGAGCTAA GTATGTGAGA CTATCATATG ATACCAAACC TGAAATCATT 
CTGCAACTTC TGCTTAAAGA ATGGCAAATG GAGTTACCCA AACTTGTTAT TTCTGTACAT 
GGAGGCATGC AGAAATTTGA ACTTCATCCA AGAATCAAGC AGTTGCTTGG AAAGGGTCTT 
ATTAAAGCTG CAGTTACAAC CGGAGCTTGG ATTTTAACTG GAGGAGTAAA TACAGGTGTG 
GCAAAACATG TTGGTGATGC CCTCAAAGAA CATGCTTCCA GATCATCTCG AAAAATTTGC 
ACTATTGGAA TAGCTCCATG GGGAGTGATA GAAAACAGGA ATGATCTTGT TGGGAGAGAT 
GTGGTTGCCC CCTATCAAAC CCTATTAAAT CCCTTGAGCA AATTGAATGT TCTGAATAAT 
CTACACTCCC ATTTCATCTT GGTGGATGAC GGCACGGTTG GAAAGTATGG GGCAGAAGTC 
AGACTGAGAA GAGAACTTGA AAAAACCATT AATCAGCAAA GAATTCATGC TAGAATTGGG 
CAAGGTGTTC CTGTGGTGGC TTTGATATTT GAAGGCGGGC CAAATGTCAT CCTTACAGTA 
CTGGAGTACC TCCAGGAAAG CCCCCCCGTT CCAGTTGTTG TGTGTGAAGG GACAGGCAGA 
GCTGCAGATT TACTAGCATA TATCCATAAG CAGACAGAGG AAGGAGGGAA TCTTCCTGAT 
GCAGCAGAGC CTGATATTAT ATCAACTATC AAAAAAACAT TTAACTTTGG CCAGAGTGAA 
GCAGTTCATT TATTTCAAAC AATGATGGAG TGTATGAAAA AAAAAGAGCT TATCACTGTT 
TTCCATATTG GTTCAGAGGA CCATCAAGAT ATAGATGTGG CCATACTTAC TGCACTGCTA 
AAAGGTACTA ATGCATCAGC GTTTGACCAG CTTATCCTTA CACTGGCATG GGACAGAGTT 
GATATTGCCA AAAATCATGT ATTTGTTTAT GGACAACAGT GGTTGGTTGG ATCCTTGGAA 
CAAGCTATGC TTGATGCTCT TGTAATGGAT AGAGTTTCAT TTGTAAAACT TCTTATTGAA 
AATGGAGTAA GCATGCATAA ATTCCTTACC ATTCCTAGAC TGGAAGAACT TTATAACACT 
AAACAGGGTC CGACTAATCC AATGTTGTTC CACCTCATTC GGGATGTCAA ACAGGGTAAT 
CTTCCTCCAG GGTACAAGAT CACTCTAATT GATATAGGAC TTGTGATTGA ATATCTCATG 
GGAGGAACCT ACAGATGCAC ATACACACGA AAACGTTTTC GCTTAATATA TAATAGTCTT 
GGTGGAAATA ACCGGAGGTC AGGTCGAAAT GCTTCCAGCA GCACCCCTCA GTTGCGAAAG 
AGTCATGAAA CTTTTGGCAA TAGAGCTGAT AAAAAGGAAA AAATGAGACA CAATCATTTC 
ATTAAAACAG CCCAACCCTA CAGACCAAAG ATGGATGCAT CTATGGAAGA AGGGAAGAAG 
AAACGAACCA AAGATGAAAT TGTAGATATA GATGATCCAG AAACCAAGCG CTTTCCTTAT 
CCTCTTAATG AATTATTGAT CTGGGCTTGC CTTATGAAGA GGCAAGTCAT GGCCCGCTTT 
TTATGGCAGC ATGGTGAGGA ATCAATGGCT AAAGCATTAG TTGCCTGTAA AATCTATCGT 
TCAATGGCTT ATGAGGCAAA ACAGAGTGAC CTGGTAGATG ATACTTCAGA GGAACTGAAA 
CAGTATTCCA ATGATTTTGG TCAACTCGCC GTTGAATTAC TGGAACAGTC CTTCAGACAA 
GATGAAACAA TGGCTATGAA ATTACTCACT TATGAACTGA AAAACTGGAG TAATTCAACC 
TGCCTCAAGT TAGCAGTTTC TTCAAGGCTT AGGCCTTTTG TAGCTCACAC TTGTACACAG 
ATGCTGTTGT CTGATATGTG GATGGGACGG CTGAATATGA GAAAAAATTC CTGGTATAAG 
GTTATATTAA GCATTTTAGT TCCACCTGCC ATATTAATGC TAGAGTATAA AACCAAGGCT 
GAAATGTCCC ATATCCCACA ATCTCAAGAT GCTCATCAAA TGACCATGGA GGATAGTGAA 
AACAATTTTC ACAACATAAC AGAAGAGATA CCCATGGAAG TATTTAAAGA AGTAAAGATT 
TTGGACAGCA GTGAAGGAAA GAATGAAATG GAGATACATA TTAAATCAAA AAAGCTTCCA 
ATCACACGAA AATTTTATGC CTTTTATCAC GCGCCAATTG TAAAGTTCTG GTTTAACACA 
TTGGCATACT TAGGATTTCT GATGCTTTAT ACATTTGTAG TTCTTGTACA AATGGAACAG 
TTACCTTCAG TTCAAGAGTG GATTGTTATC GCTTATATTT TTACCTATGC TATTGAAAAA 
ATTCGTGAGG TCTTTATGTC TGAAGCTGGG AAAATCAGCC AGAAGATCAA AGTGTGGTTT 
AGTGACTACT TCAATGTCAG TGACACAATT GCCATAATTT CTTTCTTTGT TGGATTTGGA 
CTAAGATTTG GAGCAAAATG GAACTATATT AATGCATATG ATAATCATGT TTTTGTTGCT 
GGAAGATTAA TTTACTGTCT TAATATAATA TTTTGGTATG TGCGCTTGCT AGATTTTCTA 
GCTGTAAATC AACAGGCAGG ACCTTATGTA ATGATGATTG GAAAAATGGT GGCCAATATG 
TTCTACATTG TAGTGATAAT GGCTCTTGTA TTACTTAGTT TTGGTGTTCC CAGAAAAGCA 
ATACTTTATC CACATGAAGA ACCGTCTTGG TCTCTTGCTA AAGATATAGT TTTTCATCCA 
TACTGGATGA TTTTTGGTGA AGTTTATGCG TATGAAATTG ATGTGTGTGC AAATGATTCC 
GCTCTCCCGA CAATCTGTGG TCCTGGAACT TGGTTGACTC CATTTCTTCA AGCAGTCTAC 
CTCTTTGTAC AGTATATCAT TATGGTTAAT CTTCTTATTG CATTTTTCAA TAATGTATAT 
TTACAAGTGA AGGCAATTTC CAATATTGTA TGGAAGTATC AGCGATATCA TTTCATTATG 
GCTTATCATG AAAAACCAGT CCTGCCTCCT CCACTTATCA TTCTCAGCCA TATAGTTTCA 
CTGTTTTGCT GTATCTGTAA AAGAAGAAAG AAAGATAAGA CTTCTGATGG GCCAAAACTT 
TTCTTAACAG AAGAAGATCA AAAGAAACTT CATGACTTTG AAGAACAGTG TGTTGAGATG 
TATTTTGATG AGAAAGATGA CAAATTCAAT TCTGGAAGTG AAGAGAGAAT CCGTGTCACT 
TTTGAAAGAG TGGAGCAGAT GAGCATTCAG ATTAAAGAAG TTGGAGATCG TGTCAACTAC 
ATAAAAAGAT CGTTACAGTC TTTAGATTCT CAAATTGGTC ACCTGCAAGA TCTTTCAGCC 
CTAACAGTAG ATACATTAAA AACACTTACA GCCCAGAAAG CTTCAGAAGC TAGCAAAGTG 
CACAATGAGA TTACACGAGA ATTGAGTATT TCCAAACACT TGGCTCAGAA TCTTATTGAT 
GATGTTCCTG TAAGGCCTAT GTGGAAGAAG CCTAGTGTTG TAAATACACT GAGTTCCTCT 
CTTCCTCAAG GTGATCGGGA AAGTAATAAT CCTTTTCTTT GTAATATTTT TATGAAAGAT 
GAAAAAGACC CCCAGTATAA CCTGTTTGGA CAGGATTTGC CTGTGATACC CCAGAGAAAA 
GAATTCAACA TTCCAGAGGC TGGCTCCTCC TGTGGTGCCT TATTCCCAAG TGCTGTTTCT 
CCCCCAGAAT TACGACAAAG ACGACATGGG GTAGAAATGT TAAAAATATT TAATAAAAAT 
CAAAAATTAG GCAGTTCACC TAATAGTTCA CCCCATATGT CCTCCCCACC AACCAAATTT 
TCTGTGAGTA CCCCATCCCA GCCAAGTTGC AAAAGCCACT TGGAATCCAC AACCAAAGAT 
CCAGAACCCA TTTTCTATAA AGCTGCAGAA GGGGATAACA TAGAATTTGG AGCATTTGTG 
GGACACAGAG ATAGTATGGA TTTACAGAGG TTTAAAGAAA CATCAAACAA AATAAGAGAA 
CTGTTATCTA ATGATACTCC TGAAAACACT TTGAAACATG TGGGTGCTGC TGGATATAAT 
GAATGTCATA AGACTCCTAC TTCTCTTCAT TCAGAACAAG AGAGCTGTAG TAGAAGAGCG 
TCGACAGAAG ACTCTCCGGA CGTAGATTCC AGAGCAGCTT TGTTGCCGGA TTGGTTACGA 
GATAGACCAA CAAACAGAGA AATGCCATCT GAAGGAGGAA CATTAAATGG TCTTGCTTCT 
CCATTTAAAC CTGTTTTGGA TACAAATTAC TATTATTCAG CTGTGGAAAG AAATAACCTG 
ATGAGGCTGT CGCAGAGTAT TCCCTTCGTT CCTGTACCTC CACGAGGTGA GCCTGTCACA 
GTATATCGTC TGGAGGAGAG TTCTCCCAGT ATACTGAATA ACAGCATGTC TTCTTGGTCT 
CAGCTAGGCC TCTGTGCCAA AATTGAGTTT TTAAGTAAAG AGGAAATGGG AGGCGGTTTA 
CGAAGAGCAG TCAAAGTGCT GTGTACCTGG TCAGAGCACG ATGTCCTGAG ATCAGGGCAC 
CTTTATATCA TTAAGTCGTT TCTTCCAGAG GTGATAAACA CATGGTCAAG CATTTATAAA 
GAAGACACAG TTCTGCATCT CTGTCTCAGA GAAATACAAC AACAGAGAGC AGCACAGAAG 
CTCACATTTG CCTTTAATCA GATGAAACCC AAATCCATAC CATATTCTCC AAGGTTCCTG 
GAAGTTTTCC TGCTATACTG CCATTCAGCA GGGCAGTGGT TTGCTGTAGA AGAATGCATG 
ACTGGGGAAT TTAGGAAATA CAACAATAAT AATGGTGATG AAATCATTCC AACCAACACT 
CTGGAAGAGA TCATGCTAGC CTTCAGCCAC TGGACCTATG AGTACACCAG AGGGGAGTTG 
CTGGTACTTG ACTTACAAGG TGTGGGAGAA AACTTGACTG ACCCATCTGT GATAAAAGCT 
GAAGAGAAAA GATCCTGCGA CATGGTTTTT GGCCCTGCCA ATTTAGGAGA AGATGCAATA 
AAAAACTTCA GAGCCAAACA TCACTGTAAT TCTTGCTGTC GAAAGCTTAA ACTTCCAGAT 
CTGAAGAGGA ATGACTACAC ACCTGATAAA ATTATATTTC CTCAGGATGA GTCATCAGAT 
CTGAATCTTC AAGCTGGAAA TTCTACCAAA GAATCAGAAG CAACAAATTC TGTTCGTCTG 
ATGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446157.2
CDS	252..5840
Translation	MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLATKYSDVKLGEHFNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEIILQLLLKEWQMELPKLVISVHGGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVIENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTIKKTFNFGQSEAVHLFQTMMECMKKKELITVFHIGSEDHQDIDVAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVSFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLFHLIRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNASSSTPQLRKSHETFGNRADKKEKMRHNHFIKTAQPYRPKMDASMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWACLMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPAILMLEYKTKAEMSHIPQSQDAHQMTMEDSENNFHNITEEIPMEVFKEVKILDSSEGKNEMEIHIKSKKLPITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKIREVFMSEAGKISQKIKVWFSDYFNVSDTIAIISFFVGFGLRFGAKWNYINAYDNHVFVAGRLIYCLNIIFWYVRLLDFLAVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEEPSWSLAKDIVFHPYWMIFGEVYAYEIDVCANDSALPTICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIMAYHEKPVLPPPLIILSHIVSLFCCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFDEKDDKFNSGSEERIRVTFERVEQMSIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKVHNEITRELSISKHLAQNLIDDVPVRPMWKKPSVVNTLSSSLPQGDRESNNPFLCNIFMKDEKDPQYNLFGQDLPVIPQRKEFNIPEAGSSCGALFPSAVSPPELRQRRHGVEMLKIFNKNQKLGSSPNSSPHMSSPPTKFSVSTPSQPSCKSHLESTTKDPEPIFYKAAEGDNIEFGAFVGHRDSMDLQRFKETSNKIRELLSNDTPENTLKHVGAAGYNECHKTPTSLHSEQESCSRRASTEDSPDVDSRAALLPDWLRDRPTNREMPSEGGTLNGLASPFKPVLDTNYYYSAVERNNLMRLSQSIPFVPVPPRGEPVTVYRLEESSPSILNNSMSSWSQLGLCAKIEFLSKEEMGGGLRRAVKVLCTWSEHDVLRSGHLYIIKSFLPEVINTWSSIYKEDTVLHLCLREIQQQRAAQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLKRNDYTPDKIIFPQDESSDLNLQAGNSTKESEATNSVRLML

Target ORF information:

RefSeq Version	NM_053705.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transient receptor potential cation channel, subfamily M, member 7 (Trpm7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053705.2

ORF Insert Sequence:

ATGTCCCAGA AATCCTGGAT AGAGAGCACC TTGACCAAGA GGGAGTGTGT GTATATTATA 
CCAAGCTCCA AAGACCCTCA CAGATGTCTT CCAGGATGTC AGATTTGTCA GCAACTTGTC 
AGATGTTTCT GTGGTCGTTT GGTCAAGCAG CATGCATGCT TTACTGCAAG TCTTGCCACA 
AAATACTCAG ATGTGAAATT GGGTGAACAC TTTAACCAGG CAATAGAAGA ATGGTCTGTG 
GAAAAGCACA CGGAGCAGAG CCCAACAGAT GCTTATGGAG TCATCAATTT TCAAGGGGGT 
TCTCATTCCT ACAGAGCTAA GTATGTGAGA CTATCATATG ATACCAAACC TGAAATCATT 
CTGCAACTTC TGCTTAAAGA ATGGCAAATG GAGTTACCCA AACTTGTTAT TTCTGTACAT 
GGAGGCATGC AGAAATTTGA ACTTCATCCA AGAATCAAGC AGTTGCTTGG AAAGGGTCTT 
ATTAAAGCTG CAGTTACAAC CGGAGCTTGG ATTTTAACTG GAGGAGTAAA TACAGGTGTG 
GCAAAACATG TTGGTGATGC CCTCAAAGAA CATGCTTCCA GATCATCTCG AAAAATTTGC 
ACTATTGGAA TAGCTCCATG GGGAGTGATA GAAAACAGGA ATGATCTTGT TGGGAGAGAT 
GTGGTTGCCC CCTATCAAAC CCTATTAAAT CCCTTGAGCA AATTGAATGT TCTGAATAAT 
CTACACTCCC ATTTCATCTT GGTGGATGAC GGCACGGTTG GAAAGTATGG GGCAGAAGTC 
AGACTGAGAA GAGAACTTGA AAAAACCATT AATCAGCAAA GAATTCATGC TAGAATTGGG 
CAAGGTGTTC CTGTGGTGGC TTTGATATTT GAAGGCGGGC CAAATGTCAT CCTTACAGTA 
CTGGAGTACC TCCAGGAAAG CCCCCCCGTT CCAGTTGTTG TGTGTGAAGG GACAGGCAGA 
GCTGCAGATT TACTAGCATA TATCCATAAG CAGACAGAGG AAGGAGGGAA TCTTCCTGAT 
GCAGCAGAGC CTGATATTAT ATCAACTATC AAAAAAACAT TTAACTTTGG CCAGAGTGAA 
GCAGTTCATT TATTTCAAAC AATGATGGAG TGTATGAAAA AAAAAGAGCT TATCACTGTT 
TTCCATATTG GTTCAGAGGA CCATCAAGAT ATAGATGTGG CCATACTTAC TGCACTGCTA 
AAAGGTACTA ATGCATCAGC GTTTGACCAG CTTATCCTTA CACTGGCATG GGACAGAGTT 
GATATTGCCA AAAATCATGT ATTTGTTTAT GGACAACAGT GGTTGGTTGG ATCCTTGGAA 
CAAGCTATGC TTGATGCTCT TGTAATGGAT AGAGTTTCAT TTGTAAAACT TCTTATTGAA 
AATGGAGTAA GCATGCATAA ATTCCTTACC ATTCCTAGAC TGGAAGAACT TTATAACACT 
AAACAGGGTC CGACTAATCC AATGTTGTTC CACCTCATTC GGGATGTCAA ACAGGGTAAT 
CTTCCTCCAG GGTACAAGAT CACTCTAATT GATATAGGAC TTGTGATTGA ATATCTCATG 
GGAGGAACCT ACAGATGCAC ATACACACGA AAACGTTTTC GCTTAATATA TAATAGTCTT 
GGTGGAAATA ACCGGAGGTC AGGTCGAAAT GCTTCCAGCA GCACCCCTCA GTTGCGAAAG 
AGTCATGAAA CTTTTGGCAA TAGAGCTGAT AAAAAGGAAA AAATGAGACA CAATCATTTC 
ATTAAAACAG CCCAACCCTA CAGACCAAAG ATGGATGCAT CTATGGAAGA AGGGAAGAAG 
AAACGAACCA AAGATGAAAT TGTAGATATA GATGATCCAG AAACCAAGCG CTTTCCTTAT 
CCTCTTAATG AATTATTGAT CTGGGCTTGC CTTATGAAGA GGCAAGTCAT GGCCCGCTTT 
TTATGGCAGC ATGGTGAGGA ATCAATGGCT AAAGCATTAG TTGCCTGTAA AATCTATCGT 
TCAATGGCTT ATGAGGCAAA ACAGAGTGAC CTGGTAGATG ATACTTCAGA GGAACTGAAA 
CAGTATTCCA ATGATTTTGG TCAACTCGCC GTTGAATTAC TGGAACAGTC CTTCAGACAA 
GATGAAACAA TGGCTATGAA ATTACTCACT TATGAACTGA AAAACTGGAG TAATTCAACC 
TGCCTCAAGT TAGCAGTTTC TTCAAGGCTT AGGCCTTTTG TAGCTCACAC TTGTACACAG 
ATGCTGTTGT CTGATATGTG GATGGGACGG CTGAATATGA GAAAAAATTC CTGGTATAAG 
GTTATATTAA GCATTTTAGT TCCACCTGCC ATATTAATGC TAGAGTATAA AACCAAGGCT 
GAAATGTCCC ATATCCCACA ATCTCAAGAT GCTCATCAAA TGACCATGGA GGATAGTGAA 
AACAATTTTC ACAACATAAC AGAAGAGATA CCCATGGAAG TATTTAAAGA AGTAAAGATT 
TTGGACAGCA GTGAAGGAAA GAATGAAATG GAGATACATA TTAAATCAAA AAAGCTTCCA 
ATCACACGAA AATTTTATGC CTTTTATCAC GCGCCAATTG TAAAGTTCTG GTTTAACACA 
TTGGCATACT TAGGATTTCT GATGCTTTAT ACATTTGTAG TTCTTGTACA AATGGAACAG 
TTACCTTCAG TTCAAGAGTG GATTGTTATC GCTTATATTT TTACCTATGC TATTGAAAAA 
ATTCGTGAGG TCTTTATGTC TGAAGCTGGG AAAATCAGCC AGAAGATCAA AGTGTGGTTT 
AGTGACTACT TCAATGTCAG TGACACAATT GCCATAATTT CTTTCTTTGT TGGATTTGGA 
CTAAGATTTG GAGCAAAATG GAACTATATT AATGCATATG ATAATCATGT TTTTGTTGCT 
GGAAGATTAA TTTACTGTCT TAATATAATA TTTTGGTATG TGCGCTTGCT AGATTTTCTA 
GCTGTAAATC AACAGGCAGG ACCTTATGTA ATGATGATTG GAAAAATGGT GGCCAATATG 
TTCTACATTG TAGTGATAAT GGCTCTTGTA TTACTTAGTT TTGGTGTTCC CAGAAAAGCA 
ATACTTTATC CACATGAAGA ACCGTCTTGG TCTCTTGCTA AAGATATAGT TTTTCATCCA 
TACTGGATGA TTTTTGGTGA AGTTTATGCG TATGAAATTG ATGTGTGTGC AAATGATTCC 
GCTCTCCCGA CAATCTGTGG TCCTGGAACT TGGTTGACTC CATTTCTTCA AGCAGTCTAC 
CTCTTTGTAC AGTATATCAT TATGGTTAAT CTTCTTATTG CATTTTTCAA TAATGTATAT 
TTACAAGTGA AGGCAATTTC CAATATTGTA TGGAAGTATC AGCGATATCA TTTCATTATG 
GCTTATCATG AAAAACCAGT CCTGCCTCCT CCACTTATCA TTCTCAGCCA TATAGTTTCA 
CTGTTTTGCT GTATCTGTAA AAGAAGAAAG AAAGATAAGA CTTCTGATGG GCCAAAACTT 
TTCTTAACAG AAGAAGATCA AAAGAAACTT CATGACTTTG AAGAACAGTG TGTTGAGATG 
TATTTTGATG AGAAAGATGA CAAATTCAAT TCTGGAAGTG AAGAGAGAAT CCGTGTCACT 
TTTGAAAGAG TGGAGCAGAT GAGCATTCAG ATTAAAGAAG TTGGAGATCG TGTCAACTAC 
ATAAAAAGAT CGTTACAGTC TTTAGATTCT CAAATTGGTC ACCTGCAAGA TCTTTCAGCC 
CTAACAGTAG ATACATTAAA AACACTTACA GCCCAGAAAG CTTCAGAAGC TAGCAAAGTG 
CACAATGAGA TTACACGAGA ATTGAGTATT TCCAAACACT TGGCTCAGAA TCTTATTGAT 
GATGTTCCTG TAAGGCCTAT GTGGAAGAAG CCTAGTGTTG TAAATACACT GAGTTCCTCT 
CTTCCTCAAG GTGATCGGGA AAGTAATAAT CCTTTTCTTT GTAATATTTT TATGAAAGAT 
GAAAAAGACC CCCAGTATAA CCTGTTTGGA CAGGATTTGC CTGTGATACC CCAGAGAAAA 
GAATTCAACA TTCCAGAGGC TGGCTCCTCC TGTGGTGCCT TATTCCCAAG TGCTGTTTCT 
CCCCCAGAAT TACGACAAAG ACGACATGGG GTAGAAATGT TAAAAATATT TAATAAAAAT 
CAAAAATTAG GCAGTTCACC TAATAGTTCA CCCCATATGT CCTCCCCACC AACCAAATTT 
TCTGTGAGTA CCCCATCCCA GCCAAGTTGC AAAAGCCACT TGGAATCCAC AACCAAAGAT 
CCAGAACCCA TTTTCTATAA AGCTGCAGAA GGGGATAACA TAGAATTTGG AGCATTTGTG 
GGACACAGAG ATAGTATGGA TTTACAGAGG TTTAAAGAAA CATCAAACAA AATAAGAGAA 
CTGTTATCTA ATGATACTCC TGAAAACACT TTGAAACATG TGGGTGCTGC TGGATATAAT 
GAATGTCATA AGACTCCTAC TTCTCTTCAT TCAGAACAAG AGAGCTGTAG TAGAAGAGCG 
TCGACAGAAG ACTCTCCGGA CGTAGATTCC AGAGCAGCTT TGTTGCCGGA TTGGTTACGA 
GATAGACCAA CAAACAGAGA AATGCCATCT GAAGGAGGAA CATTAAATGG TCTTGCTTCT 
CCATTTAAAC CTGTTTTGGA TACAAATTAC TATTATTCAG CTGTGGAAAG AAATAACCTG 
ATGAGGCTGT CGCAGAGTAT TCCCTTCGTT CCTGTACCTC CACGAGGTGA GCCTGTCACA 
GTATATCGTC TGGAGGAGAG TTCTCCCAGT ATACTGAATA ACAGCATGTC TTCTTGGTCT 
CAGCTAGGCC TCTGTGCCAA AATTGAGTTT TTAAGTAAAG AGGAAATGGG AGGCGGTTTA 
CGAAGAGCAG TCAAAGTGCT GTGTACCTGG TCAGAGCACG ATGTCCTGAG ATCAGGGCAC 
CTTTATATCA TTAAGTCGTT TCTTCCAGAG GTGATAAACA CATGGTCAAG CATTTATAAA 
GAAGACACAG TTCTGCATCT CTGTCTCAGA GAAATACAAC AACAGAGAGC AGCACAGAAG 
CTCACATTTG CCTTTAATCA GATGAAACCC AAATCCATAC CATATTCTCC AAGGTTCCTG 
GAAGTTTTCC TGCTATACTG CCATTCAGCA GGGCAGTGGT TTGCTGTAGA AGAATGCATG 
ACTGGGGAAT TTAGGAAATA CAACAATAAT AATGGTGATG AAATCATTCC AACCAACACT 
CTGGAAGAGA TCATGCTAGC CTTCAGCCAC TGGACCTATG AGTACACCAG AGGGGAGTTG 
CTGGTACTTG ACTTACAAGG TGTGGGAGAA AACTTGACTG ACCCATCTGT GATAAAAGCT 
GAAGAGAAAA GATCCTGCGA CATGGTTTTT GGCCCTGCCA ATTTAGGAGA AGATGCAATA 
AAAAACTTCA GAGCCAAACA TCACTGTAAT TCTTGCTGTC GAAAGCTTAA ACTTCCAGAT 
CTGAAGAGGA ATGACTACAC ACCTGATAAA ATTATATTTC CTCAGGATGA GTCATCAGAT 
CTGAATCTTC AAGCTGGAAA TTCTACCAAA GAATCAGAAG CAACAAATTC TGTTCGTCTG 
ATGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of ROS-TRPM7-ERK1/2 axis in high concentration glucose-mediated proliferation and phenotype switching of rat aortic vascular smooth muscle cells.
Biochemical and biophysical research communications494(3-4)526-533(2017 12)
Yang M,Fang J,Liu Q,Wang Y,Zhang Z

A TRPM7 variant shows altered sensitivity to magnesium that may contribute to the pathogenesis of two Guamanian neurodegenerative disorders.
Proceedings of the National Academy of Sciences of the United States of America102(32)11510-5(2005 Aug)
Hermosura MC,Nayakanti H,Dorovkov MV,Calderon FR,Ryazanov AG,Haymer DS,Garruto RM

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Trpm7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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