Npy2r cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npy2r
Entrez Gene ID	66024
Full Name	neuropeptide Y receptor Y2
General protein information	Preferred Names neuropeptide Y receptor Y2 Names neuropeptide Y receptor type 2 neuropeptide Y receptor Y2-like neuropeptide Y-Y2 receptor neuropeptide Y/peptide YY-Y2 receptor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	plays a role in inhibition of fluid secretion in small intestine epithelial crypt cells; mediates inhibition of cAMP production [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008761113.2	XP_008759335.1	neuropeptide Y receptor type 2 isoform X1
NM_023968.1	NP_076458.1	neuropeptide Y receptor type 2
XM_006232534.3	XP_006232596.1	neuropeptide Y receptor type 2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NPY2R	NP_001012655.1
Anopheles gambiae (African malaria mosquito)	GPRNPY3	XP_320180.1
Homo sapiens (human)	NPY2R	NP_000901.1
Xenopus tropicalis (tropical clawed frog)	npy2r	XP_004911210.1
Bos taurus (cattle)	NPY2R	NP_776826.1
Danio rerio (zebrafish)	npy2rl	XP_001332759.1
Danio rerio (zebrafish)	LOC100003852	XP_001343301.2
Canis lupus familiaris (dog)	NPY2R	XP_005629430.1
Mus musculus (house mouse)	Npy2r	NP_032757.2
Gallus gallus (chicken)	NPY2R	NP_001026299.1
Danio rerio (zebrafish)	si:ch73-24a15.1	XP_003197663.1
Caenorhabditis elegans (roundworm)	npr-6	NP_509725.2
	NPY2R	NP_001028004.1
Rattus norvegicus (Norway rat)	Npy2r	NP_076458.1

Gene Ontology
Bibliography

Related articles in PubMed

Non-neuronal neuropeptide Y and its receptors during acute rejection of rat pulmonary allografts.
Schmitz J, Zakrzewicz A, Wilker S, Kuncov? J, Hecker A, Grau V, Padberg W, Holler JPN
Transplant immunology43-4449-53(2017 Aug)

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
Badgandi HB, Hwang SH, Shimada IS, Loriot E, Mukhopadhyay S
The Journal of cell biology216(3)743-760(2017 Mar)

Neuropeptide Y Y2 and Y5 receptors as promising targets for neuroprotection in primary neurons exposed to oxygen-glucose deprivation and in transient focal cerebral ischemia in rats.
Domin H, Przykaza ?, Jantas D, Kozniewska E, Boguszewski PM, ?mia?owska M
Neuroscience344305-325(2017 Mar)

Effective G-protein coupling of Y2 receptors along axonal fiber tracts and its relevance for epilepsy.
Dum E, F?rtinger S, Gasser E, Bukovac A, Drexel M, Tasan R, Sperk G
Neuropeptides6149-55(2017 Feb)

Limbic but not non-limbic kindling impairs conditioned fear and promotes plasticity of NPY and its Y2 receptor.
Botterill JJ, Guskjolen AJ, Marks WN, Caruncho HJ, Kalynchuk LE
Brain structure & function220(6)3641-55(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NPY and agonists of Y2R and Y5R may be neuroprotective against oxygen-glucose deprivation-induced neuronal cell death in primary cortical cell cultures after delayed treatment. A Y2R agonist not only diminished transient cerebral ischemia-induced neuronal injury, but also improved functional outcome after delayed treatment. Y5 and especially Y2 receptors may be promising targets for neuroprotection against ischemic damage
Title: Neuropeptide Y Y2 and Y5 receptors as promising targets for neuroprotection in primary neurons exposed to oxygen-glucose deprivation and in transient focal cerebral ischemia in rats.

Our data indicate that Y2 receptors are not only located at nerve terminals but also along fiber tracts and are there functionally coupled to G-proteins.
Title: Effective G-protein coupling of Y2 receptors along axonal fiber tracts and its relevance for epilepsy.

In the hippocampus and mossy fiber pathway, limbic kindling-induced de novo expression of the NPY Y2 receptor.
Title: Limbic but not non-limbic kindling impairs conditioned fear and promotes plasticity of NPY and its Y2 receptor.

The blockade of peripheral NPY Y2 receptor by specific antagonist had no effect on baroreceptor reflex, baseline Systolic blood pressure and heart rate in STZ-induced diabetic rats with hyperlipidemia.
Title: Blockage of peripheral NPY Y1 and Y2 receptors modulates barorefex sensitivity of diabetic rats.

Data suggest that effects of neuropeptide Y (NPY) on hepatic VLDL-triglyceride secretion (i.e., occur via NPY receptor 2) can be dissociated from effects on feeding behavior (i.e., occur via NPY receptor 1); studies used selective agonists.
Title: Central nervous system neuropeptide Y signaling via the Y1 receptor partially dissociates feeding behavior from lipoprotein metabolism in lean rats.

The following Npy2r gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npy2r cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42256	NM_023968.1 Latest version!	Rattus norvegicus neuropeptide Y receptor Y2 (Npy2r), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa24808	XM_006232534.3 Latest version!	Rattus norvegicus neuropeptide Y receptor Y2 (Npy2r), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa24808	XM_008761113.2 Latest version!	Rattus norvegicus neuropeptide Y receptor Y2 (Npy2r), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npy2r ( NM_023968.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_076458.1 Rattus norvegicus neuropeptide Y receptor Y2 (Npy2r), mRNA.

Price & Availability↑

CloneID	ORa42256
Clone ID Related Accession (Same CDS sequence)	NM_023968.1
Accession Version	NM_023968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	neuropeptide Y receptor type 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY004257.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY004257.1 [ECO:0000332] ##Evidence-Data-END##

ATGGGCCCAT TAGGTGCAGA GGCAGATGAG AATCAAACTG TAGAAGTGAA AGTGGAACTC 
TATGGGTCGG GGCCCACCAC TCCTAGAGGT GAGTTGCCCC CTGATCCAGA GCCGGAGCTC 
ATAGACAGCA CCAAACTGGT TGAGGTGCAG GTGGTCCTTA TACTGGCCTA TTGTTCCATC 
ATCTTGCTGG GCGTAGTTGG CAACTCTCTG GTAATCCATG TGGTGATCAA ATTCAAGAGC 
ATGCGCACAG TAACCAACTT TTTTATTGCC AACCTGGCTG TGGCGGATCT TTTGGTGAAC 
ACCCTGTGCC TGCCATTCAC TCTTACCTAT ACCTTGATGG GGGAGTGGAA AATGGGTCCA 
GTTTTGTGCC ATTTGGTGCC CTATGCCCAG GGTCTGGCAG TACAAGTGTC CACAATAACT 
TTGACAGTCA TTGCTTTGGA CCGACATCGT TGCATTGTCT ACCACCTGGA GAGCAAGATC 
TCCAAGCAAA TCAGCTTCCT GATTATTGGC CTGGCGTGGG GTGTCAGCGC TCTGCTGGCA 
AGTCCCCTTG CCATCTTCCG GGAGTACTCA CTGATTGAGA TTATTCCTGA CTTTGAGATT 
GTAGCCTGTA CTGAGAAATG GCCCGGGGAG GAGAAGAGTG TGTACGGTAC AGTCTACAGC 
CTTTCCACCC TGCTAATCCT CTACGTTTTG CCTCTGGGCA TCATATCTTT CTCCTACACC 
CGGATCTGGA GTAAGCTAAA GAACCACGTT AGTCCTGGAG CTGCAAGTGA CCATTACCAT 
CAGCGAAGGC ACAAAACGAC CAAAATGCTC GTGTGCGTGG TAGTGGTGTT TGCAGTCAGC 
TGGCTGCCCC TCCATGCCTT CCAACTTGCT GTGGACATCG ACAGCCATGT CCTGGACCTG 
AAGGAGTACA AACTCATCTT CACCGTGTTC CACATTATTG CGATGTGCTC CACCTTCGCC 
AACCCCCTTC TCTATGGCTG GATGAACAGC AACTACAGAA AAGCTTTCCT CTCAGCCTTC 
CGCTGTGAGC AGAGGTTGGA TGCCATTCAC TCGGAGGTGT CCATGACCTT CAAGGCTAAA 
AAGAACCTGG AAGTCAAAAA GAACAATGGC CTCACTGACT CTTTTTCAGA GGCCACCAAC 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076458.1
CDS	1..1146
Translation	MGPLGAEADENQTVEVKVELYGSGPTTPRGELPPDPEPELIDSTKLVEVQVVLILAYCSIILLGVVGNSLVIHVVIKFKSMRTVTNFFIANLAVADLLVNTLCLPFTLTYTLMGEWKMGPVLCHLVPYAQGLAVQVSTITLTVIALDRHRCIVYHLESKISKQISFLIIGLAWGVSALLASPLAIFREYSLIEIIPDFEIVACTEKWPGEEKSVYGTVYSLSTLLILYVLPLGIISFSYTRIWSKLKNHVSPGAASDHYHQRRHKTTKMLVCVVVVFAVSWLPLHAFQLAVDIDSHVLDLKEYKLIFTVFHIIAMCSTFANPLLYGWMNSNYRKAFLSAFRCEQRLDAIHSEVSMTFKAKKNLEVKKNNGLTDSFSEATNV

Target ORF information:

RefSeq Version	NM_023968.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuropeptide Y receptor Y2 (Npy2r), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023968.1

ORF Insert Sequence:

ATGGGCCCAT TAGGTGCAGA GGCAGATGAG AATCAAACTG TAGAAGTGAA AGTGGAACTC 
TATGGGTCGG GGCCCACCAC TCCTAGAGGT GAGTTGCCCC CTGATCCAGA GCCGGAGCTC 
ATAGACAGCA CCAAACTGGT TGAGGTGCAG GTGGTCCTTA TACTGGCCTA TTGTTCCATC 
ATCTTGCTGG GCGTAGTTGG CAACTCTCTG GTAATCCATG TGGTGATCAA ATTCAAGAGC 
ATGCGCACAG TAACCAACTT TTTTATTGCC AACCTGGCTG TGGCGGATCT TTTGGTGAAC 
ACCCTGTGCC TGCCATTCAC TCTTACCTAT ACCTTGATGG GGGAGTGGAA AATGGGTCCA 
GTTTTGTGCC ATTTGGTGCC CTATGCCCAG GGTCTGGCAG TACAAGTGTC CACAATAACT 
TTGACAGTCA TTGCTTTGGA CCGACATCGT TGCATTGTCT ACCACCTGGA GAGCAAGATC 
TCCAAGCAAA TCAGCTTCCT GATTATTGGC CTGGCGTGGG GTGTCAGCGC TCTGCTGGCA 
AGTCCCCTTG CCATCTTCCG GGAGTACTCA CTGATTGAGA TTATTCCTGA CTTTGAGATT 
GTAGCCTGTA CTGAGAAATG GCCCGGGGAG GAGAAGAGTG TGTACGGTAC AGTCTACAGC 
CTTTCCACCC TGCTAATCCT CTACGTTTTG CCTCTGGGCA TCATATCTTT CTCCTACACC 
CGGATCTGGA GTAAGCTAAA GAACCACGTT AGTCCTGGAG CTGCAAGTGA CCATTACCAT 
CAGCGAAGGC ACAAAACGAC CAAAATGCTC GTGTGCGTGG TAGTGGTGTT TGCAGTCAGC 
TGGCTGCCCC TCCATGCCTT CCAACTTGCT GTGGACATCG ACAGCCATGT CCTGGACCTG 
AAGGAGTACA AACTCATCTT CACCGTGTTC CACATTATTG CGATGTGCTC CACCTTCGCC 
AACCCCCTTC TCTATGGCTG GATGAACAGC AACTACAGAA AAGCTTTCCT CTCAGCCTTC 
CGCTGTGAGC AGAGGTTGGA TGCCATTCAC TCGGAGGTGT CCATGACCTT CAAGGCTAAA 
AAGAACCTGG AAGTCAAAAA GAACAATGGC CTCACTGACT CTTTTTCAGA GGCCACCAAC 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npy2r ( XM_008761113.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759335.1 Rattus norvegicus neuropeptide Y receptor Y2 (Npy2r), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24808
Clone ID Related Accession (Same CDS sequence)	XM_008761113.2 , XM_006232534.3
Accession Version	XM_008761113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuropeptide Y receptor type 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCAT TAGGTGCAGA GGCAGATGAG AATCAAACTG TAGAAGTGAA AGTGGAACTC 
TATGGGTCGG GGCCCACCAC TCCTAGAGGT GAGTTGCCCC CTGATCCAGA GCCGGAGCTC 
ATAGACAGCA CCAAACTGGT TGAGGTGCAG GTGGTCCTTA TATTGGCCTA TTGTTCCATC 
ATCTTGCTGG GCGTAGTTGG CAACTCTCTG GTAATCCATG TGGTGATCAA ATTCAAGAGC 
ATGCGCACAG TAACCAACTT TTTTATTGCC AACCTGGCTG TGGCGGATCT TTTGGTGAAC 
ACCCTGTGCC TGCCATTCAC TCTTACCTAT ACCTTGATGG GGGAGTGGAA AATGGGTCCA 
GTTTTGTGCC ATTTGGTGCC CTATGCCCAG GGTCTGGCAG TACAAGTGTC CACAATAACT 
TTGACAGTCA TTGCTTTGGA CCGACATCGT TGCATTGTCT ACCACCTGGA GAGCAAGATC 
TCCAAGCAAA TCAGCTTCCT GATTATTGGC CTGGCGTGGG GTGTCAGCGC TCTGCTGGCA 
AGTCCCCTTG CCATCTTCCG GGAGTACTCA CTGATTGAGA TTATTCCTGA CTTTGAGATT 
GTAGCCTGTA CTGAGAAATG GCCCGGGGAG GAGAAGAGTG TGTACGGTAC AGTCTACAGC 
CTTTCCACCC TGCTAATCCT CTACGTTTTG CCTCTGGGCA TCATATCTTT CTCCTACACC 
CGGATCTGGA GTAAGCTAAA GAACCACGTT AGTCCTGGAG CTGCAAGTGA CCATTACCAT 
CAGCGAAGGC ACAAAACGAC CAAAATGCTC GTGTGCGTGG TAGTGGTGTT TGCAGTCAGC 
TGGCTGCCCC TCCATGCCTT CCAACTTGCT GTGGACATCG ACAGCCATGT CCTGGACCTG 
AAGGAGTACA AACTCATCTT CACCGTGTTC CACATTATTG CGATGTGCTC CACCTTCGCC 
AACCCCCTTC TCTATGGCTG GATGAACAGC AACTACAGAA AAGCTTTCCT CTCAGCCTTC 
CGCTGTGAGC AGAGGTTGGA TGCCATTCAC TCGGAGGTGT CCATGACCTT CAAGGCTAAA 
AAGAACCTGG AAGTCAAAAA GAACAATGGC CTCACTGACT CTTTTTCAGA GGCCACCAAC 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759335.1
CDS	114..1259
Translation	MGPLGAEADENQTVEVKVELYGSGPTTPRGELPPDPEPELIDSTKLVEVQVVLILAYCSIILLGVVGNSLVIHVVIKFKSMRTVTNFFIANLAVADLLVNTLCLPFTLTYTLMGEWKMGPVLCHLVPYAQGLAVQVSTITLTVIALDRHRCIVYHLESKISKQISFLIIGLAWGVSALLASPLAIFREYSLIEIIPDFEIVACTEKWPGEEKSVYGTVYSLSTLLILYVLPLGIISFSYTRIWSKLKNHVSPGAASDHYHQRRHKTTKMLVCVVVVFAVSWLPLHAFQLAVDIDSHVLDLKEYKLIFTVFHIIAMCSTFANPLLYGWMNSNYRKAFLSAFRCEQRLDAIHSEVSMTFKAKKNLEVKKNNGLTDSFSEATNV

Target ORF information:

RefSeq Version	XM_008761113.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuropeptide Y receptor Y2 (Npy2r), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761113.2

ORF Insert Sequence:

ATGGGCCCAT TAGGTGCAGA GGCAGATGAG AATCAAACTG TAGAAGTGAA AGTGGAACTC 
TATGGGTCGG GGCCCACCAC TCCTAGAGGT GAGTTGCCCC CTGATCCAGA GCCGGAGCTC 
ATAGACAGCA CCAAACTGGT TGAGGTGCAG GTGGTCCTTA TATTGGCCTA TTGTTCCATC 
ATCTTGCTGG GCGTAGTTGG CAACTCTCTG GTAATCCATG TGGTGATCAA ATTCAAGAGC 
ATGCGCACAG TAACCAACTT TTTTATTGCC AACCTGGCTG TGGCGGATCT TTTGGTGAAC 
ACCCTGTGCC TGCCATTCAC TCTTACCTAT ACCTTGATGG GGGAGTGGAA AATGGGTCCA 
GTTTTGTGCC ATTTGGTGCC CTATGCCCAG GGTCTGGCAG TACAAGTGTC CACAATAACT 
TTGACAGTCA TTGCTTTGGA CCGACATCGT TGCATTGTCT ACCACCTGGA GAGCAAGATC 
TCCAAGCAAA TCAGCTTCCT GATTATTGGC CTGGCGTGGG GTGTCAGCGC TCTGCTGGCA 
AGTCCCCTTG CCATCTTCCG GGAGTACTCA CTGATTGAGA TTATTCCTGA CTTTGAGATT 
GTAGCCTGTA CTGAGAAATG GCCCGGGGAG GAGAAGAGTG TGTACGGTAC AGTCTACAGC 
CTTTCCACCC TGCTAATCCT CTACGTTTTG CCTCTGGGCA TCATATCTTT CTCCTACACC 
CGGATCTGGA GTAAGCTAAA GAACCACGTT AGTCCTGGAG CTGCAAGTGA CCATTACCAT 
CAGCGAAGGC ACAAAACGAC CAAAATGCTC GTGTGCGTGG TAGTGGTGTT TGCAGTCAGC 
TGGCTGCCCC TCCATGCCTT CCAACTTGCT GTGGACATCG ACAGCCATGT CCTGGACCTG 
AAGGAGTACA AACTCATCTT CACCGTGTTC CACATTATTG CGATGTGCTC CACCTTCGCC 
AACCCCCTTC TCTATGGCTG GATGAACAGC AACTACAGAA AAGCTTTCCT CTCAGCCTTC 
CGCTGTGAGC AGAGGTTGGA TGCCATTCAC TCGGAGGTGT CCATGACCTT CAAGGCTAAA 
AAGAACCTGG AAGTCAAAAA GAACAATGGC CTCACTGACT CTTTTTCAGA GGCCACCAAC 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npy2r ( XM_006232534.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232596.1 Rattus norvegicus neuropeptide Y receptor Y2 (Npy2r), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa24808
Clone ID Related Accession (Same CDS sequence)	XM_008761113.2 , XM_006232534.3
Accession Version	XM_006232534.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuropeptide Y receptor type 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232534.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCAT TAGGTGCAGA GGCAGATGAG AATCAAACTG TAGAAGTGAA AGTGGAACTC 
TATGGGTCGG GGCCCACCAC TCCTAGAGGT GAGTTGCCCC CTGATCCAGA GCCGGAGCTC 
ATAGACAGCA CCAAACTGGT TGAGGTGCAG GTGGTCCTTA TATTGGCCTA TTGTTCCATC 
ATCTTGCTGG GCGTAGTTGG CAACTCTCTG GTAATCCATG TGGTGATCAA ATTCAAGAGC 
ATGCGCACAG TAACCAACTT TTTTATTGCC AACCTGGCTG TGGCGGATCT TTTGGTGAAC 
ACCCTGTGCC TGCCATTCAC TCTTACCTAT ACCTTGATGG GGGAGTGGAA AATGGGTCCA 
GTTTTGTGCC ATTTGGTGCC CTATGCCCAG GGTCTGGCAG TACAAGTGTC CACAATAACT 
TTGACAGTCA TTGCTTTGGA CCGACATCGT TGCATTGTCT ACCACCTGGA GAGCAAGATC 
TCCAAGCAAA TCAGCTTCCT GATTATTGGC CTGGCGTGGG GTGTCAGCGC TCTGCTGGCA 
AGTCCCCTTG CCATCTTCCG GGAGTACTCA CTGATTGAGA TTATTCCTGA CTTTGAGATT 
GTAGCCTGTA CTGAGAAATG GCCCGGGGAG GAGAAGAGTG TGTACGGTAC AGTCTACAGC 
CTTTCCACCC TGCTAATCCT CTACGTTTTG CCTCTGGGCA TCATATCTTT CTCCTACACC 
CGGATCTGGA GTAAGCTAAA GAACCACGTT AGTCCTGGAG CTGCAAGTGA CCATTACCAT 
CAGCGAAGGC ACAAAACGAC CAAAATGCTC GTGTGCGTGG TAGTGGTGTT TGCAGTCAGC 
TGGCTGCCCC TCCATGCCTT CCAACTTGCT GTGGACATCG ACAGCCATGT CCTGGACCTG 
AAGGAGTACA AACTCATCTT CACCGTGTTC CACATTATTG CGATGTGCTC CACCTTCGCC 
AACCCCCTTC TCTATGGCTG GATGAACAGC AACTACAGAA AAGCTTTCCT CTCAGCCTTC 
CGCTGTGAGC AGAGGTTGGA TGCCATTCAC TCGGAGGTGT CCATGACCTT CAAGGCTAAA 
AAGAACCTGG AAGTCAAAAA GAACAATGGC CTCACTGACT CTTTTTCAGA GGCCACCAAC 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232596.1
CDS	265..1410
Translation	MGPLGAEADENQTVEVKVELYGSGPTTPRGELPPDPEPELIDSTKLVEVQVVLILAYCSIILLGVVGNSLVIHVVIKFKSMRTVTNFFIANLAVADLLVNTLCLPFTLTYTLMGEWKMGPVLCHLVPYAQGLAVQVSTITLTVIALDRHRCIVYHLESKISKQISFLIIGLAWGVSALLASPLAIFREYSLIEIIPDFEIVACTEKWPGEEKSVYGTVYSLSTLLILYVLPLGIISFSYTRIWSKLKNHVSPGAASDHYHQRRHKTTKMLVCVVVVFAVSWLPLHAFQLAVDIDSHVLDLKEYKLIFTVFHIIAMCSTFANPLLYGWMNSNYRKAFLSAFRCEQRLDAIHSEVSMTFKAKKNLEVKKNNGLTDSFSEATNV

Target ORF information:

RefSeq Version	XM_006232534.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuropeptide Y receptor Y2 (Npy2r), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232534.3

ORF Insert Sequence:

ATGGGCCCAT TAGGTGCAGA GGCAGATGAG AATCAAACTG TAGAAGTGAA AGTGGAACTC 
TATGGGTCGG GGCCCACCAC TCCTAGAGGT GAGTTGCCCC CTGATCCAGA GCCGGAGCTC 
ATAGACAGCA CCAAACTGGT TGAGGTGCAG GTGGTCCTTA TATTGGCCTA TTGTTCCATC 
ATCTTGCTGG GCGTAGTTGG CAACTCTCTG GTAATCCATG TGGTGATCAA ATTCAAGAGC 
ATGCGCACAG TAACCAACTT TTTTATTGCC AACCTGGCTG TGGCGGATCT TTTGGTGAAC 
ACCCTGTGCC TGCCATTCAC TCTTACCTAT ACCTTGATGG GGGAGTGGAA AATGGGTCCA 
GTTTTGTGCC ATTTGGTGCC CTATGCCCAG GGTCTGGCAG TACAAGTGTC CACAATAACT 
TTGACAGTCA TTGCTTTGGA CCGACATCGT TGCATTGTCT ACCACCTGGA GAGCAAGATC 
TCCAAGCAAA TCAGCTTCCT GATTATTGGC CTGGCGTGGG GTGTCAGCGC TCTGCTGGCA 
AGTCCCCTTG CCATCTTCCG GGAGTACTCA CTGATTGAGA TTATTCCTGA CTTTGAGATT 
GTAGCCTGTA CTGAGAAATG GCCCGGGGAG GAGAAGAGTG TGTACGGTAC AGTCTACAGC 
CTTTCCACCC TGCTAATCCT CTACGTTTTG CCTCTGGGCA TCATATCTTT CTCCTACACC 
CGGATCTGGA GTAAGCTAAA GAACCACGTT AGTCCTGGAG CTGCAAGTGA CCATTACCAT 
CAGCGAAGGC ACAAAACGAC CAAAATGCTC GTGTGCGTGG TAGTGGTGTT TGCAGTCAGC 
TGGCTGCCCC TCCATGCCTT CCAACTTGCT GTGGACATCG ACAGCCATGT CCTGGACCTG 
AAGGAGTACA AACTCATCTT CACCGTGTTC CACATTATTG CGATGTGCTC CACCTTCGCC 
AACCCCCTTC TCTATGGCTG GATGAACAGC AACTACAGAA AAGCTTTCCT CTCAGCCTTC 
CGCTGTGAGC AGAGGTTGGA TGCCATTCAC TCGGAGGTGT CCATGACCTT CAAGGCTAAA 
AAGAACCTGG AAGTCAAAAA GAACAATGGC CTCACTGACT CTTTTTCAGA GGCCACCAAC 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Non-neuronal neuropeptide Y and its receptors during acute rejection of rat pulmonary allografts.
Transplant immunology43-4449-53(2017 Aug)
Schmitz J,Zakrzewicz A,Wilker S,Kuncov? J,Hecker A,Grau V,Padberg W,Holler JPN

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
The Journal of cell biology216(3)743-760(2017 Mar)
Badgandi HB,Hwang SH,Shimada IS,Loriot E,Mukhopadhyay S

Neuropeptide Y Y2 and Y5 receptors as promising targets for neuroprotection in primary neurons exposed to oxygen-glucose deprivation and in transient focal cerebral ischemia in rats.
Neuroscience344305-325(2017 Mar)
Domin H,Przykaza ?,Jantas D,Kozniewska E,Boguszewski PM,?mia?owska M

Effective G-protein coupling of Y2 receptors along axonal fiber tracts and its relevance for epilepsy.
Neuropeptides6149-55(2017 Feb)
Dum E,F?rtinger S,Gasser E,Bukovac A,Drexel M,Tasan R,Sperk G

Limbic but not non-limbic kindling impairs conditioned fear and promotes plasticity of NPY and its Y2 receptor.
Brain structure & function220(6)3641-55(2015 Nov)
Botterill JJ,Guskjolen AJ,Marks WN,Caruncho HJ,Kalynchuk LE

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Npy2r cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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