Arhgef9 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arhgef9
Entrez Gene ID	66013
Full Name	Cdc42 guanine nucleotide exchange factor 9
General protein information	Preferred Names Cdc42 guanine nucleotide exchange factor 9 Names rho guanine nucleotide exchange factor 9 Cdc42 guanine nucleotide exchange factor (GEF) 9 collybistin I rac/Cdc42 guanine nucleotide exchange factor 9
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq22

mRNA and Protein(s)

mRNA	Protein	Name
NM_023957.1	NP_076447.1	rho guanine nucleotide exchange factor 9

Pathways from BioSystems

Homologies

	LOC707387	XP_002808582.1
Danio rerio (zebrafish)	LOC559868	XP_005165263.1
Danio rerio (zebrafish)	arhgef9b	NP_001155135.1
Bos taurus (cattle)	LOC537718	NP_001030495.1
Gallus gallus (chicken)	ARHGEF9	XP_004940839.1
Pan troglodytes (chimpanzee)	ARHGEF9	XP_003954058.1
Rattus norvegicus (Norway rat)	Arhgef9	NP_076447.1
Danio rerio (zebrafish)	LOC100334379	XP_005157227.1
Xenopus tropicalis (tropical clawed frog)	arhgef9	NP_001096426.2
Homo sapiens (human)	ARHGEF9	NP_056000.1
Canis lupus familiaris (dog)	ARHGEF9	XP_005641467.1
Mus musculus (house mouse)	Arhgef9	NP_001028501.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of an elaborate complex mediating postsynaptic inhibition.
Uezu A, Kanak DJ, Bradshaw TW, Soderblom EJ, Catavero CM, Burette AC, Weinberg RJ, Soderling SH
Science (New York, N.Y.)353(6304)1123-9(2016 09)

Collybistin activation by GTP-TC10 enhances postsynaptic gephyrin clustering and hippocampal GABAergic neurotransmission.
Mayer S, Kumar R, Jaiswal M, Soykan T, Ahmadian MR, Brose N, Betz H, Rhee JS, Papadopoulos T
Proceedings of the National Academy of Sciences of the United States of America110(51)20795-800(2013 Dec)

Phosphorylation of gephyrin in hippocampal neurons by cyclin-dependent kinase CDK5 at Ser-270 is dependent on collybistin.
Kuhse J, Kalbouneh H, Schlicksupp A, M?kusch S, Nawrotzki R, Kirsch J
The Journal of biological chemistry287(37)30952-66(2012 Sep)

Effects of distinct collybistin isoforms on the formation of GABAergic synapses in hippocampal neurons.
K?rber C, Richter A, Kaiser M, Schlicksupp A, M?kusch S, Kuner T, Kirsch J, Kuhse J
Molecular and cellular neurosciences50(3-4)250-9(2012 Jul)

Selective localization of collybistin at a subset of inhibitory synapses in brain circuits.
Patrizi A, Viltono L, Frola E, Harvey K, Harvey RJ, Sasso?-Pognetto M
The Journal of comparative neurology520(1)130-41(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The enhancement of Cb-induced gephyrin clustering by GTP-TC10 does not depend on the guanine nucleotide exchange activity of Cb but involves an interaction that resembles reported interactions of other small GTPases with their effectors
Title: Collybistin activation by GTP-TC10 enhances postsynaptic gephyrin clustering and hippocampal GABAergic neurotransmission.

Phosphorylation of gephyrin in hippocampal neurons by cyclin-dependent kinase CDK5 at Ser-270 is dependent on collybistin.
Title: Phosphorylation of gephyrin in hippocampal neurons by cyclin-dependent kinase CDK5 at Ser-270 is dependent on collybistin.

Collybistin (Cb) knock-down induced impairment in GABAergic neurotransmission could be rescued by the expression of any of the Cb isoforms, independent of their C-termini or the presence of the SH3-domain in the N-terminal region.
Title: Effects of distinct collybistin isoforms on the formation of GABAergic synapses in hippocampal neurons.

data show that collybistin co-clusters with gephyrin and GABA(A) Rs in synaptic puncta and is recruited to postsynaptic specializations early during synapse development; it co-localizes with GABA(A) Rs containing the alpha1, alpha2, or alpha3 subunits
Title: Selective localization of collybistin at a subset of inhibitory synapses in brain circuits.

in transfected hippocampal neurons, all collybistin isoforms (CB2(SH3+), CB2(SH3-), CB3(SH3+), and CB3(SH3-)) target to and concentrate at GABAergic postsynapses
Title: Differential regulation of the postsynaptic clustering of ?-aminobutyric acid type A (GABAA) receptors by collybistin isoforms.

The following Arhgef9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgef9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12104	NM_023957.1 Latest version!	Rattus norvegicus Cdc42 guanine nucleotide exchange factor 9 (Arhgef9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arhgef9 ( NM_023957.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_076447.1 Rattus norvegicus Cdc42 guanine nucleotide exchange factor 9 (Arhgef9), mRNA.

Price & Availability↑

CloneID	ORa12104
Clone ID Related Accession (Same CDS sequence)	NM_023957.1
Accession Version	NM_023957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	rho guanine nucleotide exchange factor 9
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ250425.1. On or before Jun 25, 2006 this sequence version replaced XM_001057140.1, XM_001057197.1, XM_001057076.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ250425.1 [ECO:0000332] ##Evidence-Data-END##

ATGCAGTGGA TTAGAGGCGG ATCGGGAATG CTGATCACTG GAGATTCCAT CGTTAGTGCT 
GAGGCAGTAT GGGATCACGT CACCATGGCC AACCGGGGAG TTGCATTTAA GGCTGGCGAC 
GTCATCAAAG TCTTGGATGC TTCCAACAAG GATTGGTGGT GGGGCCAGAT CGACGATGAG 
GAGGGATGGT TTCCTGCCAG CTTTGTGAGG CTCTGGGTGA ACCAGGAGGA TGGGGTGGAG 
GAAGGGCCCA GTGACGTGCA GAATGGGCAC CTGGACCCCA ACTCAGACTG CCTCTGTCTG 
GGCCGGCCAC TACAGAACCG GGACCAGATG CGGGCCAATG TCATCAATGA GATCATGAGC 
ACGGAACGTC ATTACATCAA GCATCTCAAG GACATTTGCG AGGGGTACCT GAAGCAGTGC 
CGAAAGAGAA GGGACATGTT CAGTGATGAG CAGCTGAAAG TCATCTTTGG AAACATTGAA 
GACATCTACA GATTTCAGAT GGGCTTCGTG AGAGACTTGG AGAAGCAGTA CAACAATGAT 
GACCCCCACC TCAGCGAGAT AGGACCCTGC TTCTTGGAGC ATCAAGATGG CTTCTGGATA 
TACTCTGAGT ATTGTAACAA CCACCTGGAT GCCTGCATGG AGCTCTCCAA ACTGATGAAG 
GACAGCCGCT ATCAACACTT CTTTGAGGCC TGCCGCCTCT TGCAGCAGAT GATCGACATC 
GCTATCGATG GTTTTCTTTT GACTCCAGTA CAGAAGATCT GCAAATACCC CTTACAATTG 
GCCGAGCTCC TAAAGTATAC TGCCCAAGAC CACAGTGATT ACAGATATGT CGCAGCTGCT 
TTGGCTGTCA TGAGAAATGT GACTCAGCAG ATCAATGAAC GCAAGCGACG TCTGGAGAAT 
ATTGACAAGA TTGCTCAATG GCAGGCCTCT GTCCTAGACT GGGAGGGAGA TGACATCTTA 
GACAGGAGCT CGGAACTGAT CTACACTGGA GAGATGGCCT GGATCTACCA GCCCTATGGC 
CGCAACCAGC AGAGAGTCTT CTTCCTGTTT GACCACCAGA TGGTCCTCTG CAAGAAGGAC 
CTAATCCGGA GAGACATCCT ATACTACAAG GGGCGCATTG ACATGGATAA ATATGAGGTC 
ATTGACATTG AAGATGGCAG AGATGATGAC TTTAATGTCA GCATGAAAAA TGCTTTCAAG 
CTGCACAACA AGGAAACCGA AGAAGTACAT CTGTTCTTTG CCAAGAAACT GGAGGAGAAA 
ATACGTTGGC TTAGAGCTTT CAGAGAAGAG AGGAAAATGG TACAAGAAGA TGAAAAAATT 
GGCTTTGAGA TTTCTGAAAA TCAGAAGAGA CAGGCTGCAA TGACTGTGAG AAAAGCGTCT 
AAACAGAAAG GTAGGGTTGG AGAGGAAGAG AACCAAAGTC TAGAACTGAA AAGAGCTTGT 
GAGGTTCTTC AGAGATTATG GTCTCCTGGA AAGAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076447.1
CDS	383..1864
Translation	MQWIRGGSGMLITGDSIVSAEAVWDHVTMANRGVAFKAGDVIKVLDASNKDWWWGQIDDEEGWFPASFVRLWVNQEDGVEEGPSDVQNGHLDPNSDCLCLGRPLQNRDQMRANVINEIMSTERHYIKHLKDICEGYLKQCRKRRDMFSDEQLKVIFGNIEDIYRFQMGFVRDLEKQYNNDDPHLSEIGPCFLEHQDGFWIYSEYCNNHLDACMELSKLMKDSRYQHFFEACRLLQQMIDIAIDGFLLTPVQKICKYPLQLAELLKYTAQDHSDYRYVAAALAVMRNVTQQINERKRRLENIDKIAQWQASVLDWEGDDILDRSSELIYTGEMAWIYQPYGRNQQRVFFLFDHQMVLCKKDLIRRDILYYKGRIDMDKYEVIDIEDGRDDDFNVSMKNAFKLHNKETEEVHLFFAKKLEEKIRWLRAFREERKMVQEDEKIGFEISENQKRQAAMTVRKASKQKGRVGEEENQSLELKRACEVLQRLWSPGKKS

Target ORF information:

RefSeq Version	NM_023957.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Cdc42 guanine nucleotide exchange factor 9 (Arhgef9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023957.1

ORF Insert Sequence:

ATGCAGTGGA TTAGAGGCGG ATCGGGAATG CTGATCACTG GAGATTCCAT CGTTAGTGCT 
GAGGCAGTAT GGGATCACGT CACCATGGCC AACCGGGGAG TTGCATTTAA GGCTGGCGAC 
GTCATCAAAG TCTTGGATGC TTCCAACAAG GATTGGTGGT GGGGCCAGAT CGACGATGAG 
GAGGGATGGT TTCCTGCCAG CTTTGTGAGG CTCTGGGTGA ACCAGGAGGA TGGGGTGGAG 
GAAGGGCCCA GTGACGTGCA GAATGGGCAC CTGGACCCCA ACTCAGACTG CCTCTGTCTG 
GGCCGGCCAC TACAGAACCG GGACCAGATG CGGGCCAATG TCATCAATGA GATCATGAGC 
ACGGAACGTC ATTACATCAA GCATCTCAAG GACATTTGCG AGGGGTACCT GAAGCAGTGC 
CGAAAGAGAA GGGACATGTT CAGTGATGAG CAGCTGAAAG TCATCTTTGG AAACATTGAA 
GACATCTACA GATTTCAGAT GGGCTTCGTG AGAGACTTGG AGAAGCAGTA CAACAATGAT 
GACCCCCACC TCAGCGAGAT AGGACCCTGC TTCTTGGAGC ATCAAGATGG CTTCTGGATA 
TACTCTGAGT ATTGTAACAA CCACCTGGAT GCCTGCATGG AGCTCTCCAA ACTGATGAAG 
GACAGCCGCT ATCAACACTT CTTTGAGGCC TGCCGCCTCT TGCAGCAGAT GATCGACATC 
GCTATCGATG GTTTTCTTTT GACTCCAGTA CAGAAGATCT GCAAATACCC CTTACAATTG 
GCCGAGCTCC TAAAGTATAC TGCCCAAGAC CACAGTGATT ACAGATATGT CGCAGCTGCT 
TTGGCTGTCA TGAGAAATGT GACTCAGCAG ATCAATGAAC GCAAGCGACG TCTGGAGAAT 
ATTGACAAGA TTGCTCAATG GCAGGCCTCT GTCCTAGACT GGGAGGGAGA TGACATCTTA 
GACAGGAGCT CGGAACTGAT CTACACTGGA GAGATGGCCT GGATCTACCA GCCCTATGGC 
CGCAACCAGC AGAGAGTCTT CTTCCTGTTT GACCACCAGA TGGTCCTCTG CAAGAAGGAC 
CTAATCCGGA GAGACATCCT ATACTACAAG GGGCGCATTG ACATGGATAA ATATGAGGTC 
ATTGACATTG AAGATGGCAG AGATGATGAC TTTAATGTCA GCATGAAAAA TGCTTTCAAG 
CTGCACAACA AGGAAACCGA AGAAGTACAT CTGTTCTTTG CCAAGAAACT GGAGGAGAAA 
ATACGTTGGC TTAGAGCTTT CAGAGAAGAG AGGAAAATGG TACAAGAAGA TGAAAAAATT 
GGCTTTGAGA TTTCTGAAAA TCAGAAGAGA CAGGCTGCAA TGACTGTGAG AAAAGCGTCT 
AAACAGAAAG GTAGGGTTGG AGAGGAAGAG AACCAAAGTC TAGAACTGAA AAGAGCTTGT 
GAGGTTCTTC AGAGATTATG GTCTCCTGGA AAGAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of an elaborate complex mediating postsynaptic inhibition.
Science (New York, N.Y.)353(6304)1123-9(2016 09)
Uezu A,Kanak DJ,Bradshaw TW,Soderblom EJ,Catavero CM,Burette AC,Weinberg RJ,Soderling SH

Collybistin activation by GTP-TC10 enhances postsynaptic gephyrin clustering and hippocampal GABAergic neurotransmission.
Proceedings of the National Academy of Sciences of the United States of America110(51)20795-800(2013 Dec)
Mayer S,Kumar R,Jaiswal M,Soykan T,Ahmadian MR,Brose N,Betz H,Rhee JS,Papadopoulos T

Phosphorylation of gephyrin in hippocampal neurons by cyclin-dependent kinase CDK5 at Ser-270 is dependent on collybistin.
The Journal of biological chemistry287(37)30952-66(2012 Sep)
Kuhse J,Kalbouneh H,Schlicksupp A,M?kusch S,Nawrotzki R,Kirsch J

Effects of distinct collybistin isoforms on the formation of GABAergic synapses in hippocampal neurons.
Molecular and cellular neurosciences50(3-4)250-9(2012 Jul)
K?rber C,Richter A,Kaiser M,Schlicksupp A,M?kusch S,Kuner T,Kirsch J,Kuhse J

Selective localization of collybistin at a subset of inhibitory synapses in brain circuits.
The Journal of comparative neurology520(1)130-41(2012 Jan)
Patrizi A,Viltono L,Frola E,Harvey K,Harvey RJ,Sasso?-Pognetto M

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Arhgef9 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報