Nkx6-1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nkx6-1
Entrez Gene ID	65193
Full Name	NK6 homeobox 1
Synonyms	Nkx6.1,Nkx61,Nkx6a
General protein information	Preferred Names NK6 homeobox 1 Names homeobox protein Nkx-6.1 NK homeobox, family 6, A NK6 transcription factor homolog A NK6 transcription factor related, locus 1 homeobox protein NK-6 homolog A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	pancreatic beta-cell-specific transcription factor important for the formation of the insulin-producing beta-cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031737.1	NP_113925.1	homeobox protein Nkx-6.1

Pathways from BioSystems

Homologies

	NKX6-1	XP_001104458.1
Canis lupus familiaris (dog)	NKX6-1	XP_544960.2
Homo sapiens (human)	NKX6-1	NP_006159.2
Bos taurus (cattle)	NKX6-1	NP_001193167.1
Rattus norvegicus (Norway rat)	Nkx6-1	NP_113925.1
Danio rerio (zebrafish)	nkx6.1	NP_001002475.1
Pan troglodytes (chimpanzee)	NKX6-1	XP_003310328.1
Mus musculus (house mouse)	Nkx6-1	NP_659204.1
Xenopus tropicalis (tropical clawed frog)	nkx6-1	NP_001016583.2

Gene Ontology
Bibliography

Related articles in PubMed

Nkx6.1 decline accompanies mitochondrial DNA reduction but subtle nucleoid size decrease in pancreatic islet ?-cells of diabetic Goto Kakizaki rats.
?pa?ek T, Pavluch V, Al?n L, Capkov? N, Engstov? H, Dlaskov? A, Berkov? Z, Saudek F, Je?ek P
Scientific reports7(1)15674(2017 Nov)

Nkx6.1-mediated insulin secretion and ?-cell proliferation is dependent on upregulation of c-Fos.
Ray JD, Kener KB, Bitner BF, Wright BJ, Ballard MS, Barrett EJ, Hill JT, Moss LG, Tessem JS
FEBS letters590(12)1791-803(2016 06)

Aurora Kinase A is critical for the Nkx6.1 mediated ?-cell proliferation pathway.
Hobson A, Draney C, Stratford A, Becker TC, Lu D, Arlotto M, Tessem JS
Islets7(1)e1027854(2015)

Nkx6.1 regulates islet ?-cell proliferation via Nr4a1 and Nr4a3 nuclear receptors.
Tessem JS, Moss LG, Chao LC, Arlotto M, Lu D, Jensen MV, Stephens SB, Tontonoz P, Hohmeier HE, Newgard CB
Proceedings of the National Academy of Sciences of the United States of America111(14)5242-7(2014 Apr)

Control of astrocyte progenitor specification, migration and maturation by Nkx6.1 homeodomain transcription factor.
Zhao X, Chen Y, Zhu Q, Huang H, Teng P, Zheng K, Hu X, Xie B, Zhang Z, Sander M, Qiu M
PloS one9(10)e109171(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data demonstrate that c-Fos is critical for Nkx6.1-mediated expansion of functional beta-cell mass.
Title: Nkx6.1-mediated insulin secretion and ?-cell proliferation is dependent on upregulation of c-Fos.

The homeobox beta-cell transcription factor Nkx6.1 induces beta-cell proliferation by activating the orphan nuclear receptors Nr4a1 and Nr4a3.
Title: Aurora Kinase A is critical for the Nkx6.1 mediated ?-cell proliferation pathway.

Nkx6.1 and Nr4a receptors induce components of the anaphase-promoting complex, including ubiquitin-conjugating enzyme E2C, resulting in degradation of the cell cycle inhibitor p21
Title: Nkx6.1 regulates islet ?-cell proliferation via Nr4a1 and Nr4a3 nuclear receptors.

Nkx6.1 plays a vital role in astrocyte specification and differentiation in the ventral spinal cord.
Title: Control of astrocyte progenitor specification, migration and maturation by Nkx6.1 homeodomain transcription factor.

K cells could be reprogrammed partially to beta-cells through the combined expression of Nkx6.1 and Neurogenin3, and reaggregation in suspension culture.
Title: Reprogramming of enteroendocrine K cells to pancreatic ?-cells through the combined expression of Nkx6.1 and Neurogenin3, and reaggregation in suspension culture.

The following Nkx6-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nkx6-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12549	NM_031737.1 Latest version!	Rattus norvegicus NK6 homeobox 1 (Nkx6-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nkx6-1 ( NM_031737.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113925.1 Rattus norvegicus NK6 homeobox 1 (Nkx6-1), mRNA.

Price & Availability↑

CloneID	ORa12549
Clone ID Related Accession (Same CDS sequence)	NM_031737.1
Accession Version	NM_031737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	homeobox protein Nkx-6.1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF004431.1. On Dec 17, 2003 this sequence version replaced XM_346455.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF004431.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348, SAMN04563759 [ECO:0000348] ##Evidence-Data-END##

ATGTTAGCTG TGGGGGCGAT GGAGGGCCCC CGGCAGAGCG CGTTCCTGCT CAGCAGCCCG 
CCCTTGGCCG CCCTGCACAG CATGGCCGAG ATGAAGACCC CGCTCTACCC TGCCGCCTAT 
CCCCCGCTGC CCACCGGGCC CCCCTCCTCC TCGTCCTCGT CCTCCTCGTC CTCGTCGCCC 
TCCCCACCTT TGGGCGCACA CAACCCGGGC GGTTTGAAGC CCCCGGCCGC GGGGGGCCTC 
TCGTCCCTGG GCAGCCCCCC GCAGCAGCTT TCGGCGGCCA CTCCGCACGG CATCAACGAC 
ATCCTGAGCC GGCCCTCCAT GCCGGTGGCC TCGGGGGCCG CCCTGCCCTC CGCCTCGCCC 
TCGGGGTCTT CTTCCTCCTC CTCCTCGTCC GCCTCCGCCA CCTCGGCCTC TGCGGCCGCC 
GCTGCTGCTG CCGCTGCCGC CGCCGCTGCC GCCTCATCGC CCGCCGGGCT GTTGGCCGGC 
CTGCCCCGCT TCAGCAGCCT GAGCCCTCCG CCACCGCCGC CCGGGCTCTA CTTTAGCCCC 
AGCGCCGCGG CCGTGGCCGC CGTGGGCCGG TACCCCAAGC CCCTGGCCGA GCTGCCCGGT 
CGGACGCCCA TCTTCTGGCC TGGGGTGATG CAGAGCCCGC CTTGGAGGGA CGCGCGCCTT 
GCCTGTACCC CCCATCAAGG ATCCATTTTG TTGGACAAAG ATGGGAAGAG AAAACACACC 
AGACCCACAT TCTCCGGCCA GCAGATCTTC GCCCTGGAGA AGACTTTCGA ACAAACGAAG 
TACTTGGCAG GACCAGAGAG AGCACGCTTG GCCTATTCTC TGGGGATGAC GGAGAGTCAG 
GTCAAGGTCT GGTTCCAGAA TCGGCGGACC AAGTGGAGAA AGAAGCACGC AGCCGAGATG 
GCCACCGCCA AGAAGAAGCA GGACTCGGAG ACTGAGCGGC TCAAGGGGAC TTCGGAGAAC 
GAGGAGGACG ACGACGATTA CAACAAGCCC CTGGACCCGA ACTCTGACGA CGAGAAAATC 
ACTCAGCTGC TGAAAAAGCA CAAATCCAGC GGAGGCAGCC TCCTGCTGCA CGCGTCGGAG 
GCCGAGGGCT CGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113925.1
CDS	789..1886
Translation	MLAVGAMEGPRQSAFLLSSPPLAALHSMAEMKTPLYPAAYPPLPTGPPSSSSSSSSSSSPSPPLGAHNPGGLKPPAAGGLSSLGSPPQQLSAATPHGINDILSRPSMPVASGAALPSASPSGSSSSSSSSASATSASAAAAAAAAAAAAAASSPAGLLAGLPRFSSLSPPPPPPGLYFSPSAAAVAAVGRYPKPLAELPGRTPIFWPGVMQSPPWRDARLACTPHQGSILLDKDGKRKHTRPTFSGQQIFALEKTFEQTKYLAGPERARLAYSLGMTESQVKVWFQNRRTKWRKKHAAEMATAKKKQDSETERLKGTSENEEDDDDYNKPLDPNSDDEKITQLLKKHKSSGGSLLLHASEAEGSS

Target ORF information:

RefSeq Version	NM_031737.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NK6 homeobox 1 (Nkx6-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031737.1

ORF Insert Sequence:

ATGTTAGCTG TGGGGGCGAT GGAGGGCCCC CGGCAGAGCG CGTTCCTGCT CAGCAGCCCG 
CCCTTGGCCG CCCTGCACAG CATGGCCGAG ATGAAGACCC CGCTCTACCC TGCCGCCTAT 
CCCCCGCTGC CCACCGGGCC CCCCTCCTCC TCGTCCTCGT CCTCCTCGTC CTCGTCGCCC 
TCCCCACCTT TGGGCGCACA CAACCCGGGC GGTTTGAAGC CCCCGGCCGC GGGGGGCCTC 
TCGTCCCTGG GCAGCCCCCC GCAGCAGCTT TCGGCGGCCA CTCCGCACGG CATCAACGAC 
ATCCTGAGCC GGCCCTCCAT GCCGGTGGCC TCGGGGGCCG CCCTGCCCTC CGCCTCGCCC 
TCGGGGTCTT CTTCCTCCTC CTCCTCGTCC GCCTCCGCCA CCTCGGCCTC TGCGGCCGCC 
GCTGCTGCTG CCGCTGCCGC CGCCGCTGCC GCCTCATCGC CCGCCGGGCT GTTGGCCGGC 
CTGCCCCGCT TCAGCAGCCT GAGCCCTCCG CCACCGCCGC CCGGGCTCTA CTTTAGCCCC 
AGCGCCGCGG CCGTGGCCGC CGTGGGCCGG TACCCCAAGC CCCTGGCCGA GCTGCCCGGT 
CGGACGCCCA TCTTCTGGCC TGGGGTGATG CAGAGCCCGC CTTGGAGGGA CGCGCGCCTT 
GCCTGTACCC CCCATCAAGG ATCCATTTTG TTGGACAAAG ATGGGAAGAG AAAACACACC 
AGACCCACAT TCTCCGGCCA GCAGATCTTC GCCCTGGAGA AGACTTTCGA ACAAACGAAG 
TACTTGGCAG GACCAGAGAG AGCACGCTTG GCCTATTCTC TGGGGATGAC GGAGAGTCAG 
GTCAAGGTCT GGTTCCAGAA TCGGCGGACC AAGTGGAGAA AGAAGCACGC AGCCGAGATG 
GCCACCGCCA AGAAGAAGCA GGACTCGGAG ACTGAGCGGC TCAAGGGGAC TTCGGAGAAC 
GAGGAGGACG ACGACGATTA CAACAAGCCC CTGGACCCGA ACTCTGACGA CGAGAAAATC 
ACTCAGCTGC TGAAAAAGCA CAAATCCAGC GGAGGCAGCC TCCTGCTGCA CGCGTCGGAG 
GCCGAGGGCT CGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nkx6.1 decline accompanies mitochondrial DNA reduction but subtle nucleoid size decrease in pancreatic islet ?-cells of diabetic Goto Kakizaki rats.
Scientific reports7(1)15674(2017 Nov)
?pa?ek T,Pavluch V,Al?n L,Capkov? N,Engstov? H,Dlaskov? A,Berkov? Z,Saudek F,Je?ek P

Nkx6.1-mediated insulin secretion and ?-cell proliferation is dependent on upregulation of c-Fos.
FEBS letters590(12)1791-803(2016 06)
Ray JD,Kener KB,Bitner BF,Wright BJ,Ballard MS,Barrett EJ,Hill JT,Moss LG,Tessem JS

Aurora Kinase A is critical for the Nkx6.1 mediated ?-cell proliferation pathway.
Islets7(1)e1027854(2015)
Hobson A,Draney C,Stratford A,Becker TC,Lu D,Arlotto M,Tessem JS

Nkx6.1 regulates islet ?-cell proliferation via Nr4a1 and Nr4a3 nuclear receptors.
Proceedings of the National Academy of Sciences of the United States of America111(14)5242-7(2014 Apr)
Tessem JS,Moss LG,Chao LC,Arlotto M,Lu D,Jensen MV,Stephens SB,Tontonoz P,Hohmeier HE,Newgard CB

Control of astrocyte progenitor specification, migration and maturation by Nkx6.1 homeodomain transcription factor.
PloS one9(10)e109171(2014)
Zhao X,Chen Y,Zhu Q,Huang H,Teng P,Zheng K,Hu X,Xie B,Zhang Z,Sander M,Qiu M

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Nkx6-1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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