Dnaja1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnaja1
Entrez Gene ID	65028
Full Name	DnaJ heat shock protein family (Hsp40) member A1
Synonyms	Hsj2
General protein information	Preferred Names DnaJ heat shock protein family (Hsp40) member A1 Names dnaJ homolog subfamily A member 1 DnaJ (Hsp40) homolog, subfamily A, member 1 DnaJ-like protein 2 HSJ-2 dnaJ-like protein 1 heat shock protein J2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22
Summary	interacts with the N-terminal fragment of hsc70; involved in DNA damage repair [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006238099.2	XP_006238161.1	dnaJ homolog subfamily A member 1 isoform X1
NM_022934.1	NP_075223.1	dnaJ homolog subfamily A member 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DNAJA1	NP_001233463.1
Bos taurus (cattle)	DNAJA1	NP_001015637.1
Rattus norvegicus (Norway rat)	Dnaja1	NP_075223.1
Gallus gallus (chicken)	DNAJA1	NP_001012963.1
Danio rerio (zebrafish)	dnaja1	NP_955956.1
Homo sapiens (human)	DNAJA1	NP_001530.1
Canis lupus familiaris (dog)	DNAJA1	NP_001239072.1
Xenopus tropicalis (tropical clawed frog)	dnaja1	NP_001011012.1
	DNAJA1	XP_001101766.2
Mus musculus (house mouse)	Dnaja1	NP_001158143.1

Gene Ontology
Bibliography

Related articles in PubMed

Knock-down of Hdj2/DNAJA1 co-chaperone results in an unexpected burst of tumorigenicity of C6 glioblastoma cells.
Meshalkina DA, Shevtsov MA, Dobrodumov AV, Komarova EY, Voronkina IV, Lazarev VF, Margulis BA, Guzhova IV
Oncotarget7(16)22050-63(2016 Apr)

RING finger protein RNF207, a novel regulator of cardiac excitation.
Roder K, Werdich AA, Li W, Liu M, Kim TY, Organ-Darling LE, Moshal KS, Hwang JM, Lu Y, Choi BR, MacRae CA, Koren G
The Journal of biological chemistry289(49)33730-40(2014 Dec)

Structure and function of human DnaJ homologue subfamily a member 1 (DNAJA1) and its relationship to pancreatic cancer.
Stark JL, Mehla K, Chaika N, Acton TB, Xiao R, Singh PK, Montelione GT, Powers R
Biochemistry53(8)1360-72(2014 Mar)

Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro.
Rauch JN, Gestwicki JE
The Journal of biological chemistry289(3)1402-14(2014 Jan)

CCT chaperonin complex is required for efficient delivery of anthrax toxin into the cytosol of host cells.
Slater LH, Hett EC, Clatworthy AE, Mark KG, Hung DT
Proceedings of the National Academy of Sciences of the United States of America110(24)9932-7(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of this study uncover a completely new role for the Hdj2 co-chaperone in tumorigenicity
Title: Knock-down of Hdj2/DNAJA1 co-chaperone results in an unexpected burst of tumorigenicity of C6 glioblastoma cells.

The following Dnaja1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnaja1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06451	NM_022934.1 Latest version!	Rattus norvegicus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa06451	XM_006238099.2 Latest version!	Rattus norvegicus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnaja1 ( NM_022934.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_075223.1 Rattus norvegicus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), mRNA.

Price & Availability↑

CloneID	ORa06451
Clone ID Related Accession (Same CDS sequence)	XM_006238099.2 , NM_022934.1
Accession Version	NM_022934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	dnaJ homolog subfamily A member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U53922.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U53922.1, FQ216722.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAAAG AAACTACTTA CTATGATGTT CTGGGGGTCA AACCCAATGC CACCCAGGAA 
GAATTGAAAA AGGCATATAG AAAATTGGCC TTGAAGTACC ACCCTGATAA AAATCCAAAT 
GAAGGAGAAA AGTTTAAACA GATTTCTCAA GCTTATGAAG TTCTTGCTGA TTCCAAGAAA 
AGGGAACTAT ATGATAAAGG GGGCGAGCAG GCGATTAAGG AGGGCGGAGC AGGTGGAGGT 
TTTGGCTCGC CCATGGATAT CTTTGATATG TTCTTTGGAG GAGGAGGAAG GATGCAAAGA 
GAAAGGAGAG GTAAAAATGT CGTGCATCAG CTCTCAGTGA CCTTAGAAGA CTTATATAAT 
GGTGCAACAA GGAAACTGGC TCTGCAAAAA AATGTGATTT GTGACAAATG TGAAGGCCGA 
GGTGGTAAGA AAGGAGCAGT AGAGTGCTGT CCCAACTGCC GGGGGACTGG CATGCAGATC 
AGGATTCATC AGATTGGACC AGGAATGGTT CAGCAAATTC AGTCAGTGTG CATGGAGTGC 
CAGGGTCATG GAGAACGCAT CAGTCCTAAA GACAGATGTA AAAGCTGCAA TGGAAGAAAG 
ATAGTTCGAG AGAAGAAAAT TTTAGAAGTT CATATTGATA AAGGCATGAA AGATGGTCAG 
AAGATAACAT TCCACGGTGA AGGAGACCAA GAACCAGGAC TGGAGCCAGG AGATATTATC 
ATTGTGTTAG ATCAGAAGGA CCATGCTGTT TTTACAAGGC GAGGAGAAGA CCTTTTCATG 
TGTATGGACA TACAGCTGGT TGAAGCATTG TGTGGCTTCC AAAAGCCAAT ATCTACTCTT 
GACAACCGAA CCATAGTCAT CACCTCTCAT CCAGGTCAGA TTGTCAAGCA TGGGGATATA 
AAATGTGTGC TAAATGAAGG TATGCCAATA TACCGTCGGC CATATGAAAA GGGACGTCTA 
ATCATTGAGT TTAAGGTAAA CTTTCCTGAA AATGGCTTTC TCTCTCCTGA TAAACTCTCT 
TTGCTGGAAA AACTCCTTCC TGAAAGGAAG GAAGTAGAAG AGACTGATGA AATGGATCAG 
GTAGAACTGG TGGACTTTGA TCCAAATCAG GAAAGACGGC GTCATTATAA TGGAGAAGCG 
TATGAGGATG ATGAGCATCA CCCCAGAGGT GGCGTTCAGT GTCAGACCTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075223.1
CDS	122..1315
Translation	MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLADSKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS

Target ORF information:

RefSeq Version	NM_022934.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022934.1

ORF Insert Sequence:

ATGGTGAAAG AAACTACTTA CTATGATGTT CTGGGGGTCA AACCCAATGC CACCCAGGAA 
GAATTGAAAA AGGCATATAG AAAATTGGCC TTGAAGTACC ACCCTGATAA AAATCCAAAT 
GAAGGAGAAA AGTTTAAACA GATTTCTCAA GCTTATGAAG TTCTTGCTGA TTCCAAGAAA 
AGGGAACTAT ATGATAAAGG GGGCGAGCAG GCGATTAAGG AGGGCGGAGC AGGTGGAGGT 
TTTGGCTCGC CCATGGATAT CTTTGATATG TTCTTTGGAG GAGGAGGAAG GATGCAAAGA 
GAAAGGAGAG GTAAAAATGT CGTGCATCAG CTCTCAGTGA CCTTAGAAGA CTTATATAAT 
GGTGCAACAA GGAAACTGGC TCTGCAAAAA AATGTGATTT GTGACAAATG TGAAGGCCGA 
GGTGGTAAGA AAGGAGCAGT AGAGTGCTGT CCCAACTGCC GGGGGACTGG CATGCAGATC 
AGGATTCATC AGATTGGACC AGGAATGGTT CAGCAAATTC AGTCAGTGTG CATGGAGTGC 
CAGGGTCATG GAGAACGCAT CAGTCCTAAA GACAGATGTA AAAGCTGCAA TGGAAGAAAG 
ATAGTTCGAG AGAAGAAAAT TTTAGAAGTT CATATTGATA AAGGCATGAA AGATGGTCAG 
AAGATAACAT TCCACGGTGA AGGAGACCAA GAACCAGGAC TGGAGCCAGG AGATATTATC 
ATTGTGTTAG ATCAGAAGGA CCATGCTGTT TTTACAAGGC GAGGAGAAGA CCTTTTCATG 
TGTATGGACA TACAGCTGGT TGAAGCATTG TGTGGCTTCC AAAAGCCAAT ATCTACTCTT 
GACAACCGAA CCATAGTCAT CACCTCTCAT CCAGGTCAGA TTGTCAAGCA TGGGGATATA 
AAATGTGTGC TAAATGAAGG TATGCCAATA TACCGTCGGC CATATGAAAA GGGACGTCTA 
ATCATTGAGT TTAAGGTAAA CTTTCCTGAA AATGGCTTTC TCTCTCCTGA TAAACTCTCT 
TTGCTGGAAA AACTCCTTCC TGAAAGGAAG GAAGTAGAAG AGACTGATGA AATGGATCAG 
GTAGAACTGG TGGACTTTGA TCCAAATCAG GAAAGACGGC GTCATTATAA TGGAGAAGCG 
TATGAGGATG ATGAGCATCA CCCCAGAGGT GGCGTTCAGT GTCAGACCTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnaja1 ( XM_006238099.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006238161.1 Rattus norvegicus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa06451
Clone ID Related Accession (Same CDS sequence)	XM_006238099.2 , NM_022934.1
Accession Version	XM_006238099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dnaJ homolog subfamily A member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAG AAACTACTTA CTATGATGTT CTGGGGGTCA AACCCAATGC CACCCAGGAA 
GAATTGAAAA AGGCATATAG AAAATTGGCC TTGAAGTACC ACCCTGATAA AAATCCAAAT 
GAAGGAGAAA AGTTTAAACA GATTTCTCAA GCTTATGAAG TTCTTGCTGA TTCCAAGAAA 
AGGGAACTAT ATGATAAAGG GGGCGAGCAG GCGATTAAGG AGGGCGGAGC AGGTGGAGGT 
TTTGGCTCGC CCATGGATAT CTTTGATATG TTCTTTGGAG GAGGAGGAAG GATGCAAAGA 
GAAAGGAGAG GTAAAAATGT CGTGCATCAG CTCTCAGTGA CCTTAGAAGA CTTATATAAT 
GGTGCAACAA GGAAACTGGC TCTGCAAAAA AATGTGATTT GTGACAAATG TGAAGGCCGA 
GGTGGTAAGA AAGGAGCAGT AGAGTGCTGT CCCAACTGCC GGGGGACTGG CATGCAGATC 
AGGATTCATC AGATTGGACC AGGAATGGTT CAGCAAATTC AGTCAGTGTG CATGGAGTGC 
CAGGGTCATG GAGAACGCAT CAGTCCTAAA GACAGATGTA AAAGCTGCAA TGGAAGAAAG 
ATAGTTCGAG AGAAGAAAAT TTTAGAAGTT CATATTGATA AAGGCATGAA AGATGGTCAG 
AAGATAACAT TCCACGGTGA AGGAGACCAA GAACCAGGAC TGGAGCCAGG AGATATTATC 
ATTGTGTTAG ATCAGAAGGA CCATGCTGTT TTTACAAGGC GAGGAGAAGA CCTTTTCATG 
TGTATGGACA TACAGCTGGT TGAAGCATTG TGTGGCTTCC AAAAGCCAAT ATCTACTCTT 
GACAACCGAA CCATAGTCAT CACCTCTCAT CCAGGTCAGA TTGTCAAGCA TGGGGATATA 
AAATGTGTGC TAAATGAAGG TATGCCAATA TACCGTCGGC CATATGAAAA GGGACGTCTA 
ATCATTGAGT TTAAGGTAAA CTTTCCTGAA AATGGCTTTC TCTCTCCTGA TAAACTCTCT 
TTGCTGGAAA AACTCCTTCC TGAAAGGAAG GAAGTAGAAG AGACTGATGA AATGGATCAG 
GTAGAACTGG TGGACTTTGA TCCAAATCAG GAAAGACGGC GTCATTATAA TGGAGAAGCG 
TATGAGGATG ATGAGCATCA CCCCAGAGGT GGCGTTCAGT GTCAGACCTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006238161.1
CDS	220..1413
Translation	MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLADSKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS

Target ORF information:

RefSeq Version	XM_006238099.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238099.2

ORF Insert Sequence:

ATGGTGAAAG AAACTACTTA CTATGATGTT CTGGGGGTCA AACCCAATGC CACCCAGGAA 
GAATTGAAAA AGGCATATAG AAAATTGGCC TTGAAGTACC ACCCTGATAA AAATCCAAAT 
GAAGGAGAAA AGTTTAAACA GATTTCTCAA GCTTATGAAG TTCTTGCTGA TTCCAAGAAA 
AGGGAACTAT ATGATAAAGG GGGCGAGCAG GCGATTAAGG AGGGCGGAGC AGGTGGAGGT 
TTTGGCTCGC CCATGGATAT CTTTGATATG TTCTTTGGAG GAGGAGGAAG GATGCAAAGA 
GAAAGGAGAG GTAAAAATGT CGTGCATCAG CTCTCAGTGA CCTTAGAAGA CTTATATAAT 
GGTGCAACAA GGAAACTGGC TCTGCAAAAA AATGTGATTT GTGACAAATG TGAAGGCCGA 
GGTGGTAAGA AAGGAGCAGT AGAGTGCTGT CCCAACTGCC GGGGGACTGG CATGCAGATC 
AGGATTCATC AGATTGGACC AGGAATGGTT CAGCAAATTC AGTCAGTGTG CATGGAGTGC 
CAGGGTCATG GAGAACGCAT CAGTCCTAAA GACAGATGTA AAAGCTGCAA TGGAAGAAAG 
ATAGTTCGAG AGAAGAAAAT TTTAGAAGTT CATATTGATA AAGGCATGAA AGATGGTCAG 
AAGATAACAT TCCACGGTGA AGGAGACCAA GAACCAGGAC TGGAGCCAGG AGATATTATC 
ATTGTGTTAG ATCAGAAGGA CCATGCTGTT TTTACAAGGC GAGGAGAAGA CCTTTTCATG 
TGTATGGACA TACAGCTGGT TGAAGCATTG TGTGGCTTCC AAAAGCCAAT ATCTACTCTT 
GACAACCGAA CCATAGTCAT CACCTCTCAT CCAGGTCAGA TTGTCAAGCA TGGGGATATA 
AAATGTGTGC TAAATGAAGG TATGCCAATA TACCGTCGGC CATATGAAAA GGGACGTCTA 
ATCATTGAGT TTAAGGTAAA CTTTCCTGAA AATGGCTTTC TCTCTCCTGA TAAACTCTCT 
TTGCTGGAAA AACTCCTTCC TGAAAGGAAG GAAGTAGAAG AGACTGATGA AATGGATCAG 
GTAGAACTGG TGGACTTTGA TCCAAATCAG GAAAGACGGC GTCATTATAA TGGAGAAGCG 
TATGAGGATG ATGAGCATCA CCCCAGAGGT GGCGTTCAGT GTCAGACCTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Knock-down of Hdj2/DNAJA1 co-chaperone results in an unexpected burst of tumorigenicity of C6 glioblastoma cells.
Oncotarget7(16)22050-63(2016 Apr)
Meshalkina DA,Shevtsov MA,Dobrodumov AV,Komarova EY,Voronkina IV,Lazarev VF,Margulis BA,Guzhova IV

RING finger protein RNF207, a novel regulator of cardiac excitation.
The Journal of biological chemistry289(49)33730-40(2014 Dec)
Roder K,Werdich AA,Li W,Liu M,Kim TY,Organ-Darling LE,Moshal KS,Hwang JM,Lu Y,Choi BR,MacRae CA,Koren G

Structure and function of human DnaJ homologue subfamily a member 1 (DNAJA1) and its relationship to pancreatic cancer.
Biochemistry53(8)1360-72(2014 Mar)
Stark JL,Mehla K,Chaika N,Acton TB,Xiao R,Singh PK,Montelione GT,Powers R

Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro.
The Journal of biological chemistry289(3)1402-14(2014 Jan)
Rauch JN,Gestwicki JE

CCT chaperonin complex is required for efficient delivery of anthrax toxin into the cytosol of host cells.
Proceedings of the National Academy of Sciences of the United States of America110(24)9932-7(2013 Jun)
Slater LH,Hett EC,Clatworthy AE,Mark KG,Hung DT

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Dnaja1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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