Cplx1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cplx1
Entrez Gene ID	64832
Full Name	complexin 1
General protein information	Preferred Names complexin 1 Names complexin-1 CPX I complexin I synaphin-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	binds the assembled SNARE (soluble N-ethylmaleimide-sensitive factor attached protein receptor) complex; modulates synaptic vesicle exocytosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022864.3	NP_074055.1	complexin-1
XM_006250571.3	XP_006250633.1	complexin-1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cplx1	NP_074055.1
	CPLX1	XP_001094482.1
Bos taurus (cattle)	CPLX1	NP_001071571.1
Mus musculus (house mouse)	Cplx1	NP_031782.3
Gallus gallus (chicken)	CPLX2	XP_424869.1
Xenopus tropicalis (tropical clawed frog)	cplx1	NP_001096263.1
Homo sapiens (human)	CPLX1	NP_006642.1
Canis lupus familiaris (dog)	CPLX1	XP_005618765.1
Danio rerio (zebrafish)	LOC563082	XP_005173398.1

Gene Ontology
Bibliography

Related articles in PubMed

C-terminal domain of mammalian complexin-1 localizes to highly curved membranes.
Gong J, Lai Y, Li X, Wang M, Leitz J, Hu Y, Zhang Y, Choi UB, Cipriano D, Pfuetzner RA, S?dhof TC, Yang X, Brunger AT, Diao J
Proceedings of the National Academy of Sciences of the United States of America113(47)E7590-E7599(2016 Nov)

Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse.
Chang S, Reim K, Pedersen M, Neher E, Brose N, Taschenberger H
The Journal of neuroscience : the official journal of the Society for Neuroscience35(21)8272-90(2015 May)

Munc18a does not alter fusion rates mediated by neuronal SNAREs, synaptotagmin, and complexin.
Zhang Y, Diao J, Colbert KN, Lai Y, Pfuetzner RA, Padolina MS, Vivona S, Ressl S, Cipriano DJ, Choi UB, Shah N, Weis WI, Brunger AT
The Journal of biological chemistry290(16)10518-34(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Munc18a does not alter synaptic fusion rates mediated by neuronal SNAREs, synaptotagmin-1, and complexin-1.
Title: Munc18a does not alter fusion rates mediated by neuronal SNAREs, synaptotagmin, and complexin.

Complexin-1 inhibits spontaneous release and synchronizes Ca2+-triggered synaptic vesicle fusion by distinct mechanisms.
Title: Complexin inhibits spontaneous release and synchronizes Ca2+-triggered synaptic vesicle fusion by distinct mechanisms.

Data show direct interaction of SNARE complex binding protein synaphin/complexin with calcium sensor synaptotagmin 1.
Title: Direct interaction of SNARE complex binding protein synaphin/complexin with calcium sensor synaptotagmin 1.

Results indicate that distinct functional domains of copmplexin I differentially regulate synaptic exocytosis, and that CplxI carries out a crucial role in fine-tuning Ca2+-triggered fast neurotransmitter release.
Title: Distinct domains of complexin I differentially regulate neurotransmitter release.

Transient increases in the levels of complexin I and complexin II proteins were detected in the injured cerebral cortex 6 h following TBI. This increase was followed by a decrease of complexin I in the injured cortex and hippocampus.
Title: Early, transient increase in complexin I and complexin II in the cerebral cortex following traumatic brain injury is attenuated by N-acetylcysteine.

The following Cplx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cplx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06397	NM_022864.3 Latest version!	Rattus norvegicus complexin 1 (Cplx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa06397	XM_006250571.3 Latest version!	Rattus norvegicus complexin 1 (Cplx1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cplx1 ( NM_022864.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_074055.1 Rattus norvegicus complexin 1 (Cplx1), mRNA.

Price & Availability↑

CloneID	ORa06397
Clone ID Related Accession (Same CDS sequence)	NM_022864.3 , XM_006250571.3
Accession Version	NM_022864.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	complexin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC093605.1. On Aug 30, 2012 this sequence version replaced NM_022864.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC093605.1, CB719168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTTCG TGATGAAACA AGCCCTGGGA GGGGCCACCA AGGATATGGG GAAGATGCTT 
GGGGGTGATG AGGAGAAGGA CCCCGATGCC GCCAAGAAGG AGGAGGAGCG GCAGGAGGCA 
CTGCGGCAGG CAGAGGAGGA GCGCAAAGCA AAGTATGCCA AGATGGAGGC AGAACGTGAG 
GTCATGCGGC AGGGCATACG AGATAAGTAT GGCATCAAGA AGAAGGAGGA GCGTGAGGCT 
GAGGCTCAGG CAGCCATGGA GGCCAACTCA GAAGGCAGCC TGACTCGACC CAAGAAGGCT 
ATCCCACCAG GCTGTGGGGA CGAGCCCGAG GAGGAAGACG AGAGCATCCT GGACACTGTC 
ATCAAGTACC TGCCTGGGCC ACTGCAGGAC ATGTTCAAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_074055.1
CDS	75..479
Translation	MEFVMKQALGGATKDMGKMLGGDEEKDPDAAKKEEERQEALRQAEEERKAKYAKMEAEREVMRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEPEEEDESILDTVIKYLPGPLQDMFKK

Target ORF information:

RefSeq Version	NM_022864.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus complexin 1 (Cplx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022864.3

ORF Insert Sequence:

ATGGAGTTCG TGATGAAACA AGCCCTGGGA GGGGCCACCA AGGATATGGG GAAGATGCTT 
GGGGGTGATG AGGAGAAGGA CCCCGATGCC GCCAAGAAGG AGGAGGAGCG GCAGGAGGCA 
CTGCGGCAGG CAGAGGAGGA GCGCAAAGCA AAGTATGCCA AGATGGAGGC AGAACGTGAG 
GTCATGCGGC AGGGCATACG AGATAAGTAT GGCATCAAGA AGAAGGAGGA GCGTGAGGCT 
GAGGCTCAGG CAGCCATGGA GGCCAACTCA GAAGGCAGCC TGACTCGACC CAAGAAGGCT 
ATCCCACCAG GCTGTGGGGA CGAGCCCGAG GAGGAAGACG AGAGCATCCT GGACACTGTC 
ATCAAGTACC TGCCTGGGCC ACTGCAGGAC ATGTTCAAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cplx1 ( XM_006250571.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250633.1 Rattus norvegicus complexin 1 (Cplx1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa06397
Clone ID Related Accession (Same CDS sequence)	NM_022864.3 , XM_006250571.3
Accession Version	XM_006250571.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	complexin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250571.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTCG TGATGAAACA AGCCCTGGGA GGGGCCACCA AGGATATGGG GAAGATGCTT 
GGGGGTGATG AGGAGAAGGA CCCCGATGCC GCCAAGAAGG AGGAGGAGCG GCAGGAGGCA 
CTGCGGCAGG CAGAGGAGGA GCGCAAAGCA AAGTATGCCA AGATGGAGGC AGAACGTGAG 
GTCATGCGGC AGGGCATACG AGATAAGTAT GGCATCAAGA AGAAGGAGGA GCGTGAGGCT 
GAGGCTCAGG CAGCCATGGA GGCCAACTCA GAAGGCAGCC TGACTCGACC CAAGAAGGCT 
ATCCCACCAG GCTGTGGGGA CGAGCCCGAG GAGGAAGACG AGAGCATCCT GGACACTGTC 
ATCAAGTACC TGCCTGGGCC ACTGCAGGAC ATGTTCAAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250633.1
CDS	197..601
Translation	MEFVMKQALGGATKDMGKMLGGDEEKDPDAAKKEEERQEALRQAEEERKAKYAKMEAEREVMRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEPEEEDESILDTVIKYLPGPLQDMFKK

Target ORF information:

RefSeq Version	XM_006250571.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus complexin 1 (Cplx1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250571.3

ORF Insert Sequence:

ATGGAGTTCG TGATGAAACA AGCCCTGGGA GGGGCCACCA AGGATATGGG GAAGATGCTT 
GGGGGTGATG AGGAGAAGGA CCCCGATGCC GCCAAGAAGG AGGAGGAGCG GCAGGAGGCA 
CTGCGGCAGG CAGAGGAGGA GCGCAAAGCA AAGTATGCCA AGATGGAGGC AGAACGTGAG 
GTCATGCGGC AGGGCATACG AGATAAGTAT GGCATCAAGA AGAAGGAGGA GCGTGAGGCT 
GAGGCTCAGG CAGCCATGGA GGCCAACTCA GAAGGCAGCC TGACTCGACC CAAGAAGGCT 
ATCCCACCAG GCTGTGGGGA CGAGCCCGAG GAGGAAGACG AGAGCATCCT GGACACTGTC 
ATCAAGTACC TGCCTGGGCC ACTGCAGGAC ATGTTCAAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

C-terminal domain of mammalian complexin-1 localizes to highly curved membranes.
Proceedings of the National Academy of Sciences of the United States of America113(47)E7590-E7599(2016 Nov)
Gong J,Lai Y,Li X,Wang M,Leitz J,Hu Y,Zhang Y,Choi UB,Cipriano D,Pfuetzner RA,S?dhof TC,Yang X,Brunger AT,Diao J

Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse.
The Journal of neuroscience : the official journal of the Society for Neuroscience35(21)8272-90(2015 May)
Chang S,Reim K,Pedersen M,Neher E,Brose N,Taschenberger H

Munc18a does not alter fusion rates mediated by neuronal SNAREs, synaptotagmin, and complexin.
The Journal of biological chemistry290(16)10518-34(2015 Apr)
Zhang Y,Diao J,Colbert KN,Lai Y,Pfuetzner RA,Padolina MS,Vivona S,Ressl S,Cipriano DJ,Choi UB,Shah N,Weis WI,Brunger AT

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Cplx1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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