Rab5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rab5a
Entrez Gene ID	64633
Full Name	RAB5A, member RAS oncogene family
General protein information	Preferred Names RAB5A, member RAS oncogene family Names ras-related protein Rab-5A small GTP-binding protein rab5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q11
Summary	low molecular weight GTP binding protein; may play a role in regulation of vesicular transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022692.1	NP_073183.1	ras-related protein Rab-5A

Pathways from BioSystems

Homologies

	RAB5A	XP_001088608.1
Bos taurus (cattle)	RAB5A	NP_001069654.1
Danio rerio (zebrafish)	rab5aa	NP_958893.1
Homo sapiens (human)	RAB5A	NP_004153.2
Mus musculus (house mouse)	Rab5a	NP_080163.1
Gallus gallus (chicken)	RAB5A	NP_001006363.1
Danio rerio (zebrafish)	rab5ab	NP_957264.1
Arabidopsis thaliana (thale cress)	ARA7	NP_193699.1
Xenopus tropicalis (tropical clawed frog)	rab5a	NP_001008068.1
Canis lupus familiaris (dog)	RAB5A	NP_001003317.1
Pan troglodytes (chimpanzee)	RAB5A	XP_516319.2
Caenorhabditis elegans (roundworm)	rab-5	NP_492481.1
Oryza sativa (rice)	Os03g0666500	NP_001050846.1
Rattus norvegicus (Norway rat)	Rab5a	NP_073183.1
Arabidopsis thaliana (thale cress)	RHA1	NP_199326.1
Oryza sativa (rice)	Os12g0631100	NP_001067340.1

Gene Ontology
Bibliography

Related articles in PubMed

Rab5 and its effector FHF contribute to neuronal polarity through dynein-dependent retrieval of somatodendritic proteins from the axon.
Guo X, Far?as GG, Mattera R, Bonifacino JS
Proceedings of the National Academy of Sciences of the United States of America113(36)E5318-27(2016 09)

Lifeguard Inhibits Fas Ligand-mediated Endoplasmic Reticulum-Calcium Release Mandatory for Apoptosis in Type II Apoptotic Cells.
Urresti J, Ruiz-Meana M, Coccia E, Ar?valo JC, Castellano J, Fern?ndez-Sanz C, Galenkamp KM, Planells-Ferrer L, Moubarak RS, Llecha-Cano N, Reix S, Garc?a-Dorado D, Barneda-Zahonero B, Comella JX
The Journal of biological chemistry291(3)1221-34(2016 Jan)

Central role for PICALM in amyloid-? blood-brain barrier transcytosis and clearance.
Zhao Z, Sagare AP, Ma Q, Halliday MR, Kong P, Kisler K, Winkler EA, Ramanathan A, Kanekiyo T, Bu G, Owens NC, Rege SV, Si G, Ahuja A, Zhu D, Miller CA, Schneider JA, Maeda M, Maeda T, Sugawara T, Ichida JK, Zlokovic BV
Nature neuroscience18(7)978-87(2015 Jul)

EARP is a multisubunit tethering complex involved in endocytic recycling.
Schindler C, Chen Y, Pu J, Guo X, Bonifacino JS
Nature cell biology17(5)639-50(2015 May)

Sac2/INPP5F is an inositol 4-phosphatase that functions in the endocytic pathway.
Nakatsu F, Messa M, N?ndez R, Czapla H, Zou Y, Strittmatter SM, De Camilli P
The Journal of cell biology209(1)85-95(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.
Title: Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.

In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes.
Title: In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes.

The reduction of vesicles exhibiting directional mobility induced by Rab5 mutants indicates that GTP-GDP switching is required for maintaining directional mobility.
Title: Rab4 and Rab5 GTPase are required for directional mobility of endocytic vesicles in astrocytes.

These findings imply that caveolin-1 controls the Rab5 activity during the caveolae-mediated endocytosis.
Title: Caveolin-1 activates Rab5 and enhances endocytosis through direct interaction.

Nerve growth factor signaling down-regulates Rab5 activity to facilitate neurite outgrowth and differentiation.
Title: Nerve growth factor-mediated neurite outgrowth via regulation of Rab5.

The following Rab5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rab5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42314	NM_022692.1 Latest version!	Rattus norvegicus RAB5A, member RAS oncogene family (Rab5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rab5a ( NM_022692.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_073183.1 Rattus norvegicus RAB5A, member RAS oncogene family (Rab5a), mRNA.

Price & Availability↑

CloneID	ORa42314
Clone ID Related Accession (Same CDS sequence)	NM_022692.1
Accession Version	NM_022692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	ras-related protein Rab-5A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF072935.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF072935.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTAATC GAGGAGCAAC AAGACCCAAC GGGCCAAATA CTGGAAATAA AATATGCCAG 
TTCAAACTGG TTCTTCTAGG AGAGTCTGCT GTTGGCAAAT CAAGTCTGGT GCTTCGCTTT 
GTGAAAGGCC AATTTCATGA GTTTCAAGAG AGTACCATTG GGGCTGCCTT TCTAACCCAA 
ACTGTGTGTC TTGATGACAC AACAGTAAAA TTTGAAATAT GGGATACAGC TGGCCAAGAA 
CGGTATCATA GCCTAGCACC AATGTACTAC CGAGGAGCAC AAGCCGCCAT AGTTGTGTAT 
GATATTACAA ATGAGGAGTC CTTTTCGAGA GCAAAAAACT GGGTTAAAGA ACTTCAAAGG 
CAAGCAAGTC CTAATATTGT GATAGCTTTG TCAGGAAACA AGGCTGACTT AGCAAATAAA 
AGAGCTGTTG ACTTCCAGGA AGCACAGTCC TATGCAGATG ACAACAGCTT ATTATTTATG 
GAGACATCAG CTAAGACATC AATGAATGTA AATGAAATAT TTATGGCAAT AGCTAAAAAG 
CTGCCAAAGA ACGAACCACA AAATCCAGGC GCAAACTCAG CCAGAGGAAG AGGAGTAGAC 
CTTACTGAGC CTGCACAGCC AGCCAGGAGT CAGTGTTGTA GTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073183.1
CDS	2..649
Translation	MANRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFSRAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPAQPARSQCCSN

Target ORF information:

RefSeq Version	NM_022692.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAB5A, member RAS oncogene family (Rab5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022692.1

ORF Insert Sequence:

ATGGCTAATC GAGGAGCAAC AAGACCCAAC GGGCCAAATA CTGGAAATAA AATATGCCAG 
TTCAAACTGG TTCTTCTAGG AGAGTCTGCT GTTGGCAAAT CAAGTCTGGT GCTTCGCTTT 
GTGAAAGGCC AATTTCATGA GTTTCAAGAG AGTACCATTG GGGCTGCCTT TCTAACCCAA 
ACTGTGTGTC TTGATGACAC AACAGTAAAA TTTGAAATAT GGGATACAGC TGGCCAAGAA 
CGGTATCATA GCCTAGCACC AATGTACTAC CGAGGAGCAC AAGCCGCCAT AGTTGTGTAT 
GATATTACAA ATGAGGAGTC CTTTTCGAGA GCAAAAAACT GGGTTAAAGA ACTTCAAAGG 
CAAGCAAGTC CTAATATTGT GATAGCTTTG TCAGGAAACA AGGCTGACTT AGCAAATAAA 
AGAGCTGTTG ACTTCCAGGA AGCACAGTCC TATGCAGATG ACAACAGCTT ATTATTTATG 
GAGACATCAG CTAAGACATC AATGAATGTA AATGAAATAT TTATGGCAAT AGCTAAAAAG 
CTGCCAAAGA ACGAACCACA AAATCCAGGC GCAAACTCAG CCAGAGGAAG AGGAGTAGAC 
CTTACTGAGC CTGCACAGCC AGCCAGGAGT CAGTGTTGTA GTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rab5 and its effector FHF contribute to neuronal polarity through dynein-dependent retrieval of somatodendritic proteins from the axon.
Proceedings of the National Academy of Sciences of the United States of America113(36)E5318-27(2016 09)
Guo X,Far?as GG,Mattera R,Bonifacino JS

Lifeguard Inhibits Fas Ligand-mediated Endoplasmic Reticulum-Calcium Release Mandatory for Apoptosis in Type II Apoptotic Cells.
The Journal of biological chemistry291(3)1221-34(2016 Jan)
Urresti J,Ruiz-Meana M,Coccia E,Ar?valo JC,Castellano J,Fern?ndez-Sanz C,Galenkamp KM,Planells-Ferrer L,Moubarak RS,Llecha-Cano N,Reix S,Garc?a-Dorado D,Barneda-Zahonero B,Comella JX

Central role for PICALM in amyloid-? blood-brain barrier transcytosis and clearance.
Nature neuroscience18(7)978-87(2015 Jul)
Zhao Z,Sagare AP,Ma Q,Halliday MR,Kong P,Kisler K,Winkler EA,Ramanathan A,Kanekiyo T,Bu G,Owens NC,Rege SV,Si G,Ahuja A,Zhu D,Miller CA,Schneider JA,Maeda M,Maeda T,Sugawara T,Ichida JK,Zlokovic BV

EARP is a multisubunit tethering complex involved in endocytic recycling.
Nature cell biology17(5)639-50(2015 May)
Schindler C,Chen Y,Pu J,Guo X,Bonifacino JS

Sac2/INPP5F is an inositol 4-phosphatase that functions in the endocytic pathway.
The Journal of cell biology209(1)85-95(2015 Apr)
Nakatsu F,Messa M,N?ndez R,Czapla H,Zou Y,Strittmatter SM,De Camilli P

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Rab5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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