Prkab2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkab2
Entrez Gene ID	64562
Full Name	protein kinase AMP-activated non-catalytic subunit beta 2
General protein information	Preferred Names protein kinase AMP-activated non-catalytic subunit beta 2 Names 5'-AMP-activated protein kinase subunit beta-2 AMP-activated protein kinase beta-2 regulatory subunit AMPK beta-2 chain AMPK subunit beta-2 protein kinase, AMP-activated, beta 2 non-catalytic subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	a component of the AMPK alpha2beta2gamma1 complex which phosphorylates glycogen synthase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022627.2	NP_072149.1	5'-AMP-activated protein kinase subunit beta-2
XM_006233011.2	XP_006233073.1	5'-AMP-activated protein kinase subunit beta-2 isoform X1
XM_006233012.2	XP_006233074.1	5'-AMP-activated protein kinase subunit beta-2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRKAB2	XP_513749.2
	PRKAB2	XP_001093423.2
Bos taurus (cattle)	PRKAB2	NP_001179257.1
Rattus norvegicus (Norway rat)	Prkab2	NP_072149.1
Arabidopsis thaliana (thale cress)	AKINBETA1	NP_197615.1
Homo sapiens (human)	PRKAB2	NP_005390.1
Gallus gallus (chicken)	PRKAB2	NP_001038127.1
Caenorhabditis elegans (roundworm)	aakb-1	NP_510298.1
Canis lupus familiaris (dog)	PRKAB2	XP_005630761.1
Mus musculus (house mouse)	Prkab2	NP_892042.2
Danio rerio (zebrafish)	prkab2	NP_001124105.1
Saccharomyces cerevisiae (baker's yeast)	SIP2	NP_011307.1
Oryza sativa (rice)	Os05g0491200	NP_001055908.1
Xenopus tropicalis (tropical clawed frog)	prkab2	NP_001106572.1
Caenorhabditis elegans (roundworm)	aakb-2	NP_499446.1

Gene Ontology
Bibliography

Related articles in PubMed

Lack of Adipocyte AMPK Exacerbates Insulin Resistance and Hepatic Steatosis through Brown and Beige Adipose Tissue Function.
Mottillo EP, Desjardins EM, Crane JD, Smith BK, Green AE, Ducommun S, Henriksen TI, Rebalka IA, Razi A, Sakamoto K, Scheele C, Kemp BE, Hawke TJ, Ortega J, Granneman JG, Steinberg GR
Cell metabolism24(1)118-29(2016 Jul)

When phosphorylated at Thr148, the ?2-subunit of AMP-activated kinase does not associate with glycogen in skeletal muscle.
Xu H, Frankenberg NT, Lamb GD, Gooley PR, Stapleton DI, Murphy RM
American journal of physiology. Cell physiology311(1)C35-42(2016 Jul)

Glutathione S-transferases interact with AMP-activated protein kinase: evidence for S-glutathionylation and activation in vitro.
Klaus A, Zorman S, Berthier A, Polge C, Ramirez S, Michelland S, S?ve M, Vertommen D, Rider M, Lentze N, Auerbach D, Schlattner U
PloS one8(5)e62497(2013)

Toward an understanding of the protein interaction network of the human liver.
Wang J, Huo K, Ma L, Tang L, Li D, Huang X, Yuan Y, Li C, Wang W, Guan W, Chen H, Jin C, Wei J, Zhang W, Yang Y, Liu Q, Zhou Y, Zhang C, Wu Z, Xu W, Zhang Y, Liu T, Yu D, Zhang Y, Chen L, Zhu D, Zhong X, Kang L, Gan X, Yu X, Ma Q, Yan J, Zhou L, Liu Z, Zhu Y, Zhou T, He F, Yang X
Molecular systems biology7536(2011 Oct)

Structure of mammalian AMPK and its regulation by ADP.
Xiao B, Sanders MJ, Underwood E, Heath R, Mayer FV, Carmena D, Jing C, Walker PA, Eccleston JF, Haire LF, Saiu P, Howell SA, Aasland R, Martin SR, Carling D, Gamblin SJ
Nature472(7342)230-3(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In rat skeletal muscle, beta2-AMPK is not associated with glycogen and that activation of AMPK by muscle contraction does not dephosphorylate beta2-AMPK.
Title: When phosphorylated at Thr148, the ?2-subunit of AMP-activated kinase does not associate with glycogen in skeletal muscle.

Results suggest that p38 MAPK is not a downstream component of AMPK-mediated signaling in skeletal muscle.
Title: Dissociation of AMP-activated protein kinase and p38 mitogen-activated protein kinase signaling in skeletal muscle.

The following Prkab2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkab2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06168	NM_022627.2 Latest version!	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 2 (Prkab2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa25096	XM_006233011.2 Latest version!	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 2 (Prkab2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa25096	XM_006233012.2 Latest version!	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 2 (Prkab2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkab2 ( NM_022627.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_072149.1 Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 2 (Prkab2), mRNA.

Price & Availability↑

CloneID	ORa06168
Clone ID Related Accession (Same CDS sequence)	NM_022627.2
Accession Version	NM_022627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	5'-AMP-activated protein kinase subunit beta-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF182717.1. On Aug 30, 2012 this sequence version replaced NM_022627.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF182717.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAACA CCACCAGTGA GCGGGTGTCC GGGGAGCGCC ACGGTGCCAA GGCTGCTCGA 
GCTGAGGGTG GCGGCCATGG CCCCGGCAAG GAGCACAAGA TCATGGTGGG AAGCACCGAC 
GACCCCAGCG TCTTCAGCCT GCCGGACTCC AAGCTCCCTG GGGACAAAGA GTTTGTACCC 
TGGCAGCAGG ATTTGGATGA TTCTGTGAAG CCCACCCAGC AGGCCCGGCC TACTGTTATC 
CGCTGGTCTG AAGGGGGCAA GGAGGTCTTC ATCTCTGGGT CTTTCAACAA TTGGAGCACC 
AAGATCCCTC TGATTAAGAG TCATAATGAC TTCGTTGCCA TCCTGGATCT TCCCGAGGGA 
GAGCACCAGT ACAAGTTCTT TGTGGATGGA CAGTGGGTTC ATGATCCATC AGAGCCTGTG 
GTTACCAGTC AGCTTGGCAC AATTAATAAT TTGATCCATG TCAAGAAATC TGATTTTGAA 
GTATTTGATG CTTTAAAGTT AGATTCTATG GAAAGCTCTG AGACATCATG TCGAGACCTG 
TCCAGCTCAC CCCCAGGGCC TTATGGTCAA GAAATGTATG TGTTTCGATC TGAGGAGAGA 
TTCAAATCCC CACCCATCCT GCCCCCTCAC CTCCTCCAAG TTATTCTTAA CAAGGACACT 
AATATTTCAT GTGACCCAGC CTTACTTCCT GAGCCCAATC ATGTTATGCT GAACCATCTC 
TATGCGCTGT CCACTAAGGA CAGTGTGATG GTCCTTAGCG CAACCCATCG CTACAAGAAG 
AAGTACGTCA CCACGCTGCT GTATAAGCCC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072149.1
CDS	8..823
Translation	MGNTTSERVSGERHGAKAARAEGGGHGPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVPWQQDLDDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYVFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSTKDSVMVLSATHRYKKKYVTTLLYKPI

Target ORF information:

RefSeq Version	NM_022627.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 2 (Prkab2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022627.2

ORF Insert Sequence:

ATGGGAAACA CCACCAGTGA GCGGGTGTCC GGGGAGCGCC ACGGTGCCAA GGCTGCTCGA 
GCTGAGGGTG GCGGCCATGG CCCCGGCAAG GAGCACAAGA TCATGGTGGG AAGCACCGAC 
GACCCCAGCG TCTTCAGCCT GCCGGACTCC AAGCTCCCTG GGGACAAAGA GTTTGTACCC 
TGGCAGCAGG ATTTGGATGA TTCTGTGAAG CCCACCCAGC AGGCCCGGCC TACTGTTATC 
CGCTGGTCTG AAGGGGGCAA GGAGGTCTTC ATCTCTGGGT CTTTCAACAA TTGGAGCACC 
AAGATCCCTC TGATTAAGAG TCATAATGAC TTCGTTGCCA TCCTGGATCT TCCCGAGGGA 
GAGCACCAGT ACAAGTTCTT TGTGGATGGA CAGTGGGTTC ATGATCCATC AGAGCCTGTG 
GTTACCAGTC AGCTTGGCAC AATTAATAAT TTGATCCATG TCAAGAAATC TGATTTTGAA 
GTATTTGATG CTTTAAAGTT AGATTCTATG GAAAGCTCTG AGACATCATG TCGAGACCTG 
TCCAGCTCAC CCCCAGGGCC TTATGGTCAA GAAATGTATG TGTTTCGATC TGAGGAGAGA 
TTCAAATCCC CACCCATCCT GCCCCCTCAC CTCCTCCAAG TTATTCTTAA CAAGGACACT 
AATATTTCAT GTGACCCAGC CTTACTTCCT GAGCCCAATC ATGTTATGCT GAACCATCTC 
TATGCGCTGT CCACTAAGGA CAGTGTGATG GTCCTTAGCG CAACCCATCG CTACAAGAAG 
AAGTACGTCA CCACGCTGCT GTATAAGCCC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkab2 ( XM_006233011.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233073.1 Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 2 (Prkab2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25096
Clone ID Related Accession (Same CDS sequence)	XM_006233011.2 , XM_006233012.2
Accession Version	XM_006233011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	5'-AMP-activated protein kinase subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006233011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACA CCACCAGTGA GCGGGTGTCC GGGGAGCGCC ACGGTGCCAA GGCTGCTCGA 
GCTGAGGGTG GCGGCCATGG CCCCGGCAAG GAGCACAAGA TCATGGTGGG AAGCACCGAC 
GACCCCAGCG TCTTCAGCCT GCCGGACTCC AAGCTCCCTG GGGACAAAGA GTTTGTACCC 
TGGCAGCAGG ATTTGGATGA TTCTGTGAAG CCCACCCAGC AGGCCCGGCC TACTGTTATC 
CGCTGGTCTG AAGGGGGCAA GGAGGTCTTC ATCTCTGGGT CTTTCAACAA TTGGAGCACC 
AAGATCCCTC TGATTAAGAG TCATAATGAC TTTGTTGCCA TCCTGGATCT TCCCGAGGGA 
GAGCACCAGT ACAAGTTCTT TGTGGATGGA CAGTGGGTTC ATGATCCATC AGAGCCTGTG 
GTTACCAGTC AGCTTGGCAC AATTAATAAT TTGATCCATG TCAAGAAATC TGATTTTGAA 
GTATTTGATG CTTTAAAGTT AGATTCTATG GAAAGCTCCG AGACATCATG TCGAGACCTG 
TCCAGCTCAC CCCCAGGGCC TTATGGTCAA GAAATGTATG TGTTTCGATC TGAGGAGAGA 
TTCAAATCCC CACCCATCCT GCCCCCTCAC CTCCTCCAAG TTATTCTTAA CAAGGACACT 
AATATTTCAT GTGACCCAGC CTTACTTCCT GAGCCCAATC ATGTTATGCT GAACCATCTC 
TATGCGCTGT CCATTAAGGA CAGTGTGATG GTCCTTAGCG CAACCCATCG CTACAAGAAG 
AAGTACGTCA CCACGCTGCT GTATAAGCCC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233073.1
CDS	209..1024
Translation	MGNTTSERVSGERHGAKAARAEGGGHGPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVPWQQDLDDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYVFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPI

Target ORF information:

RefSeq Version	XM_006233011.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 2 (Prkab2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233011.2

ORF Insert Sequence:

ATGGGAAACA CCACCAGTGA GCGGGTGTCC GGGGAGCGCC ACGGTGCCAA GGCTGCTCGA 
GCTGAGGGTG GCGGCCATGG CCCCGGCAAG GAGCACAAGA TCATGGTGGG AAGCACCGAC 
GACCCCAGCG TCTTCAGCCT GCCGGACTCC AAGCTCCCTG GGGACAAAGA GTTTGTACCC 
TGGCAGCAGG ATTTGGATGA TTCTGTGAAG CCCACCCAGC AGGCCCGGCC TACTGTTATC 
CGCTGGTCTG AAGGGGGCAA GGAGGTCTTC ATCTCTGGGT CTTTCAACAA TTGGAGCACC 
AAGATCCCTC TGATTAAGAG TCATAATGAC TTTGTTGCCA TCCTGGATCT TCCCGAGGGA 
GAGCACCAGT ACAAGTTCTT TGTGGATGGA CAGTGGGTTC ATGATCCATC AGAGCCTGTG 
GTTACCAGTC AGCTTGGCAC AATTAATAAT TTGATCCATG TCAAGAAATC TGATTTTGAA 
GTATTTGATG CTTTAAAGTT AGATTCTATG GAAAGCTCCG AGACATCATG TCGAGACCTG 
TCCAGCTCAC CCCCAGGGCC TTATGGTCAA GAAATGTATG TGTTTCGATC TGAGGAGAGA 
TTCAAATCCC CACCCATCCT GCCCCCTCAC CTCCTCCAAG TTATTCTTAA CAAGGACACT 
AATATTTCAT GTGACCCAGC CTTACTTCCT GAGCCCAATC ATGTTATGCT GAACCATCTC 
TATGCGCTGT CCATTAAGGA CAGTGTGATG GTCCTTAGCG CAACCCATCG CTACAAGAAG 
AAGTACGTCA CCACGCTGCT GTATAAGCCC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkab2 ( XM_006233012.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233074.1 Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 2 (Prkab2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa25096
Clone ID Related Accession (Same CDS sequence)	XM_006233011.2 , XM_006233012.2
Accession Version	XM_006233012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	5'-AMP-activated protein kinase subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006233012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACA CCACCAGTGA GCGGGTGTCC GGGGAGCGCC ACGGTGCCAA GGCTGCTCGA 
GCTGAGGGTG GCGGCCATGG CCCCGGCAAG GAGCACAAGA TCATGGTGGG AAGCACCGAC 
GACCCCAGCG TCTTCAGCCT GCCGGACTCC AAGCTCCCTG GGGACAAAGA GTTTGTACCC 
TGGCAGCAGG ATTTGGATGA TTCTGTGAAG CCCACCCAGC AGGCCCGGCC TACTGTTATC 
CGCTGGTCTG AAGGGGGCAA GGAGGTCTTC ATCTCTGGGT CTTTCAACAA TTGGAGCACC 
AAGATCCCTC TGATTAAGAG TCATAATGAC TTTGTTGCCA TCCTGGATCT TCCCGAGGGA 
GAGCACCAGT ACAAGTTCTT TGTGGATGGA CAGTGGGTTC ATGATCCATC AGAGCCTGTG 
GTTACCAGTC AGCTTGGCAC AATTAATAAT TTGATCCATG TCAAGAAATC TGATTTTGAA 
GTATTTGATG CTTTAAAGTT AGATTCTATG GAAAGCTCCG AGACATCATG TCGAGACCTG 
TCCAGCTCAC CCCCAGGGCC TTATGGTCAA GAAATGTATG TGTTTCGATC TGAGGAGAGA 
TTCAAATCCC CACCCATCCT GCCCCCTCAC CTCCTCCAAG TTATTCTTAA CAAGGACACT 
AATATTTCAT GTGACCCAGC CTTACTTCCT GAGCCCAATC ATGTTATGCT GAACCATCTC 
TATGCGCTGT CCATTAAGGA CAGTGTGATG GTCCTTAGCG CAACCCATCG CTACAAGAAG 
AAGTACGTCA CCACGCTGCT GTATAAGCCC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233074.1
CDS	109..924
Translation	MGNTTSERVSGERHGAKAARAEGGGHGPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVPWQQDLDDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYVFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPI

Target ORF information:

RefSeq Version	XM_006233012.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit beta 2 (Prkab2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233012.2

ORF Insert Sequence:

ATGGGAAACA CCACCAGTGA GCGGGTGTCC GGGGAGCGCC ACGGTGCCAA GGCTGCTCGA 
GCTGAGGGTG GCGGCCATGG CCCCGGCAAG GAGCACAAGA TCATGGTGGG AAGCACCGAC 
GACCCCAGCG TCTTCAGCCT GCCGGACTCC AAGCTCCCTG GGGACAAAGA GTTTGTACCC 
TGGCAGCAGG ATTTGGATGA TTCTGTGAAG CCCACCCAGC AGGCCCGGCC TACTGTTATC 
CGCTGGTCTG AAGGGGGCAA GGAGGTCTTC ATCTCTGGGT CTTTCAACAA TTGGAGCACC 
AAGATCCCTC TGATTAAGAG TCATAATGAC TTTGTTGCCA TCCTGGATCT TCCCGAGGGA 
GAGCACCAGT ACAAGTTCTT TGTGGATGGA CAGTGGGTTC ATGATCCATC AGAGCCTGTG 
GTTACCAGTC AGCTTGGCAC AATTAATAAT TTGATCCATG TCAAGAAATC TGATTTTGAA 
GTATTTGATG CTTTAAAGTT AGATTCTATG GAAAGCTCCG AGACATCATG TCGAGACCTG 
TCCAGCTCAC CCCCAGGGCC TTATGGTCAA GAAATGTATG TGTTTCGATC TGAGGAGAGA 
TTCAAATCCC CACCCATCCT GCCCCCTCAC CTCCTCCAAG TTATTCTTAA CAAGGACACT 
AATATTTCAT GTGACCCAGC CTTACTTCCT GAGCCCAATC ATGTTATGCT GAACCATCTC 
TATGCGCTGT CCATTAAGGA CAGTGTGATG GTCCTTAGCG CAACCCATCG CTACAAGAAG 
AAGTACGTCA CCACGCTGCT GTATAAGCCC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lack of Adipocyte AMPK Exacerbates Insulin Resistance and Hepatic Steatosis through Brown and Beige Adipose Tissue Function.
Cell metabolism24(1)118-29(2016 Jul)
Mottillo EP,Desjardins EM,Crane JD,Smith BK,Green AE,Ducommun S,Henriksen TI,Rebalka IA,Razi A,Sakamoto K,Scheele C,Kemp BE,Hawke TJ,Ortega J,Granneman JG,Steinberg GR

When phosphorylated at Thr148, the ?2-subunit of AMP-activated kinase does not associate with glycogen in skeletal muscle.
American journal of physiology. Cell physiology311(1)C35-42(2016 Jul)
Xu H,Frankenberg NT,Lamb GD,Gooley PR,Stapleton DI,Murphy RM

Glutathione S-transferases interact with AMP-activated protein kinase: evidence for S-glutathionylation and activation in vitro.
PloS one8(5)e62497(2013)
Klaus A,Zorman S,Berthier A,Polge C,Ramirez S,Michelland S,S?ve M,Vertommen D,Rider M,Lentze N,Auerbach D,Schlattner U

Toward an understanding of the protein interaction network of the human liver.
Molecular systems biology7536(2011 Oct)
Wang J,Huo K,Ma L,Tang L,Li D,Huang X,Yuan Y,Li C,Wang W,Guan W,Chen H,Jin C,Wei J,Zhang W,Yang Y,Liu Q,Zhou Y,Zhang C,Wu Z,Xu W,Zhang Y,Liu T,Yu D,Zhang Y,Chen L,Zhu D,Zhong X,Kang L,Gan X,Yu X,Ma Q,Yan J,Zhou L,Liu Z,Zhu Y,Zhou T,He F,Yang X

Structure of mammalian AMPK and its regulation by ADP.
Nature472(7342)230-3(2011 Apr)
Xiao B,Sanders MJ,Underwood E,Heath R,Mayer FV,Carmena D,Jing C,Walker PA,Eccleston JF,Haire LF,Saiu P,Howell SA,Aasland R,Martin SR,Carling D,Gamblin SJ

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Prkab2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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