Bard1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bard1
Entrez Gene ID	64557
Full Name	BRCA1 associated RING domain 1
General protein information	Preferred Names BRCA1 associated RING domain 1 Names BRCA1-associated RING domain protein 1 BARD-1 RING-type E3 ubiquitin transferase BARD1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q33

mRNA and Protein(s)

mRNA	Protein	Name
NM_022622.1	NP_072144.1	BRCA1-associated RING domain protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	BARD1	NP_001179630.1
Xenopus tropicalis (tropical clawed frog)	bard1	XP_002932040.2
Homo sapiens (human)	BARD1	NP_000456.2
Pan troglodytes (chimpanzee)	BARD1	XP_526019.2
Rattus norvegicus (Norway rat)	Bard1	NP_072144.1
Gallus gallus (chicken)	BARD1	NP_001026417.1
	BARD1	XP_001084740.1
Canis lupus familiaris (dog)	BARD1	XP_005640797.1
Mus musculus (house mouse)	Bard1	NP_031551.1
Danio rerio (zebrafish)	LOC100147913	XP_001921099.3

Gene Ontology
Bibliography

Related articles in PubMed

The UBXN1 protein associates with autoubiquitinated forms of the BRCA1 tumor suppressor and inhibits its enzymatic function.
Wu-Baer F, Ludwig T, Baer R
Molecular and cellular biology30(11)2787-98(2010 Jun)

NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control.
Wang B, Hurov K, Hofmann K, Elledge SJ
Genes & development23(6)729-39(2009 Mar)

MERIT40 facilitates BRCA1 localization and DNA damage repair.
Feng L, Huang J, Chen J
Genes & development23(6)719-28(2009 Mar)

BRCA1-associated protein 1 interferes with BRCA1/BARD1 RING heterodimer activity.
Nishikawa H, Wu W, Koike A, Kojima R, Gomi H, Fukuda M, Ohta T
Cancer research69(1)111-9(2009 Jan)

The basal-like mammary carcinomas induced by Brca1 or Bard1 inactivation implicate the BRCA1/BARD1 heterodimer in tumor suppression.
Shakya R, Szabolcs M, McCarthy E, Ospina E, Basso K, Nandula S, Murty V, Baer R, Ludwig T
Proceedings of the National Academy of Sciences of the United States of America105(19)7040-5(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Full-length BARD1 might be involved in apoptotic control in spermatogonia and primary spermatocytes. Switch to BRCA1-independent BARD1beta might be necessary to induce apoptosis in BRCA1-expressing meiotic spermatocytes and early round spermatids.
Title: BARD1 expression during spermatogenesis is associated with apoptosis and hormonally regulated.

Assignment of the BRCA1-associated RING domain gene (Bard1) to rat chromosome 9q34 by in situ hybridization and radiation hybrid mapping
Title: Assignment of the BRCA1-associated RING domain gene (Bard1) to rat chromosome 9q34 by in situ hybridization and radiation hybrid mapping.

The following Bard1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bard1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06155	NM_022622.1 Latest version!	Rattus norvegicus BRCA1 associated RING domain 1 (Bard1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bard1 ( NM_022622.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_072144.1 Rattus norvegicus BRCA1 associated RING domain 1 (Bard1), mRNA.

Price & Availability↑

CloneID	ORa06155
Clone ID Related Accession (Same CDS sequence)	NM_022622.1
Accession Version	NM_022622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	BRCA1-associated RING domain protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF182946.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF182946.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCACGCC GGCCGCCGAG GGTCTGCTCC GGGAACAAGC CTCCTCCCGT GCCCGCCATG 
GAACCAGCTA CCGACGGGCT TTGGGCCCAC AGCCGTGCGG CGCTTGCCCG TCTGGAGAAG 
TTGTTGCGCT GCTCCCGCTG TGCTAATATT CTGAGGGAGC CCGTGTGCCT AGGAGGATGC 
GAGCACATCT TCTGTAGTGG TTGTATAAGC GACTGTGTTG GATCAGGATG CCCAGTGTGT 
CATACCCCAG CCTGGATCCT AGACCTCAAG ATAAACAGAC AGTTGGACAG CATGATCCAG 
CTTTATAGTA AGCTTCAAAA TTTGCTACAT GACAATAAAG GTTCAGATTC AAAAGACGAC 
ACATCTAGGG CAAGTTTATT TGGTGATGCA GAAAGGAAGA AGAATTCAGT AAAAATGTGG 
TTTAGTCCTC GAAGTAAGAA AATTAGATGT GTTGTGAATA AAGTTTCAGT ACAAACCCAG 
CCTCAAAAGG CAAAGGATGA CAAAGCCCAG GAAGCCTCAG TGTTTGAATT TGTTTCCGCA 
ACTCCCCCTG TAGTTGTTTC TACGAGGGCT AAAACAGCTT CAAGAACATC TGCAAAAAAG 
CATCCCAAGA AATCTGTAGC TAAGATCAAC CGGGAGGGAA ATTTCAGGCC AGAAACAAGG 
GATAGTAGAT TTGATTCCAA AGAAAAGCTG AAGGAAGAGA AGGTTGTCTC CTTTAGCCAA 
ACACTAGTTA TGGAGAATTC ACGGGTAAAT GGCGAAATAG ACTTATTAGC GAGTGGCTCT 
GTGGTAGAAT CCGTCTTCTC TGGCAGCTTT GCTGAAGTCT CTTTACCATT GGCTGAGCAT 
ATAGTGTCTC CAGATACTGT GAGCAAGAGT GAAGAGGCTC CTGAGAAGAA GGTCTGTGTA 
GAAGATCGTT GTCCAGTAGG GAGTGATGGA AATCCCAAAG GCTGCCACAG GCCTCCCACT 
TCTACTTCTA AGAAATGCGG GAGCAACGTT CCAAGCGCCA GCGGAGAAAT CCGTGAGCCA 
ACATTGCTTG CAGAAAATGT AGTGTTGGTT GACTGTTCTT CACTGCCTTC AGGCCGACTT 
CAGGTTGATG TCACCCTCAG GAGACAGAGT AACGCATCAG ATGACTCTCT TAGCCTTTCA 
CCAGGCACAC CCCCATCTCT GCTGAACAAT TCCACTCACA GACAAATGAT GTCAAAGCCC 
TCCACAGTGA AGCTGTCTTC TGGTATTCCA GCCAGGAAAA GAAATCACAG AGGAGAGACG 
TTACTGCACA TTGCCTCTAT AAAGGGTGAT ATATCTTCTG TTGAATACCT CTTGCAAAAT 
GGAAACGACC CAAATGTTAA GGACCATGCT GGATGGACAC CGTTGCATGA AGCCTGCAGT 
CATGGGCACC TGAAGATAGT GGAGCTGCTG CTCCAGCACA ATGCCTTGGT GAACACCACC 
GGCTATCACA ATGACTCGCC ACTGCACGAT GCCGCCAAGA ATGGCCACAT CGATATAGTC 
AAGGTGTTAC TGTCCCACGG AGCTTCCAGG AACGCTGTTA ACATATTTGG TGAGCGGCCA 
GTGGATTACA CAGACGCTGA GAATATAAGG TCATTATTGC TGCTGCCAGA GAAGACGGAT 
TCATTCTCAA CTAGCCAGTG CTCTGTCCAG GTGAACACCG GGCAGCGGAA GAGTGGGCCG 
CTGGTACTAA TAGGCAGTGG GCTTTCTTCA CAGCAGCAGA AACTGCTCAG CAAACTTGAG 
ACAGTGCTAA AGGCTAAGAA GTGTGCTGAG TTTGACAACA CAGTAACTCA TGTCATTGTT 
CCTGATGAGG AAGCTCAGAG TACCTTGAAG TGTATGCTTG GGATTCTCAA TGGATGCTGG 
GTCCTGAAGT TTGATTGGGT GAAAGCCTGT TTGGACAGCC AAGAACGTGA GCAGGAAGAA 
AAGTATGAAG TTCCTGGAGG TCCGCAGAGG AGCAGGCTCA ACAGAGAGCA GCTGCTGCCC 
AAACTGTTCG ATGGATGCTA CTTCTTTCTG GGGGGGAACT TCAAACATCA TCCAAAAGAA 
GACCTCCTGA AGCTCATTGC TGCAGCAGGA GGCAGAATCC TCAGCAGAAA GCCCAAGCCA 
GACAGTGACG TGACTCAGAC CATCAACACG GTTGCATACC ATGCCAAGCC TGACTCTGAT 
CAGCGCTTCT GTACGCAGTA CATTGTCTAT GAGGATCTGT TTAACTGTCA CCCAGAGAGG 
GTTCGGCAGG GCAAAGTCTG GATGGCTCCT TCCACCTGGC TAATCAGCTG TGTAATGGCC 
TTTGAGTTGC TTCCTCTTGA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072144.1
CDS	17..2323
Translation	MPRRPPRVCSGNKPPPVPAMEPATDGLWAHSRAALARLEKLLRCSRCANILREPVCLGGCEHIFCSGCISDCVGSGCPVCHTPAWILDLKINRQLDSMIQLYSKLQNLLHDNKGSDSKDDTSRASLFGDAERKKNSVKMWFSPRSKKIRCVVNKVSVQTQPQKAKDDKAQEASVFEFVSATPPVVVSTRAKTASRTSAKKHPKKSVAKINREGNFRPETRDSRFDSKEKLKEEKVVSFSQTLVMENSRVNGEIDLLASGSVVESVFSGSFAEVSLPLAEHIVSPDTVSKSEEAPEKKVCVEDRCPVGSDGNPKGCHRPPTSTSKKCGSNVPSASGEIREPTLLAENVVLVDCSSLPSGRLQVDVTLRRQSNASDDSLSLSPGTPPSLLNNSTHRQMMSKPSTVKLSSGIPARKRNHRGETLLHIASIKGDISSVEYLLQNGNDPNVKDHAGWTPLHEACSHGHLKIVELLLQHNALVNTTGYHNDSPLHDAAKNGHIDIVKVLLSHGASRNAVNIFGERPVDYTDAENIRSLLLLPEKTDSFSTSQCSVQVNTGQRKSGPLVLIGSGLSSQQQKLLSKLETVLKAKKCAEFDNTVTHVIVPDEEAQSTLKCMLGILNGCWVLKFDWVKACLDSQEREQEEKYEVPGGPQRSRLNREQLLPKLFDGCYFFLGGNFKHHPKEDLLKLIAAAGGRILSRKPKPDSDVTQTINTVAYHAKPDSDQRFCTQYIVYEDLFNCHPERVRQGKVWMAPSTWLISCVMAFELLPLDS

Target ORF information:

RefSeq Version	NM_022622.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BRCA1 associated RING domain 1 (Bard1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022622.1

ORF Insert Sequence:

ATGCCACGCC GGCCGCCGAG GGTCTGCTCC GGGAACAAGC CTCCTCCCGT GCCCGCCATG 
GAACCAGCTA CCGACGGGCT TTGGGCCCAC AGCCGTGCGG CGCTTGCCCG TCTGGAGAAG 
TTGTTGCGCT GCTCCCGCTG TGCTAATATT CTGAGGGAGC CCGTGTGCCT AGGAGGATGC 
GAGCACATCT TCTGTAGTGG TTGTATAAGC GACTGTGTTG GATCAGGATG CCCAGTGTGT 
CATACCCCAG CCTGGATCCT AGACCTCAAG ATAAACAGAC AGTTGGACAG CATGATCCAG 
CTTTATAGTA AGCTTCAAAA TTTGCTACAT GACAATAAAG GTTCAGATTC AAAAGACGAC 
ACATCTAGGG CAAGTTTATT TGGTGATGCA GAAAGGAAGA AGAATTCAGT AAAAATGTGG 
TTTAGTCCTC GAAGTAAGAA AATTAGATGT GTTGTGAATA AAGTTTCAGT ACAAACCCAG 
CCTCAAAAGG CAAAGGATGA CAAAGCCCAG GAAGCCTCAG TGTTTGAATT TGTTTCCGCA 
ACTCCCCCTG TAGTTGTTTC TACGAGGGCT AAAACAGCTT CAAGAACATC TGCAAAAAAG 
CATCCCAAGA AATCTGTAGC TAAGATCAAC CGGGAGGGAA ATTTCAGGCC AGAAACAAGG 
GATAGTAGAT TTGATTCCAA AGAAAAGCTG AAGGAAGAGA AGGTTGTCTC CTTTAGCCAA 
ACACTAGTTA TGGAGAATTC ACGGGTAAAT GGCGAAATAG ACTTATTAGC GAGTGGCTCT 
GTGGTAGAAT CCGTCTTCTC TGGCAGCTTT GCTGAAGTCT CTTTACCATT GGCTGAGCAT 
ATAGTGTCTC CAGATACTGT GAGCAAGAGT GAAGAGGCTC CTGAGAAGAA GGTCTGTGTA 
GAAGATCGTT GTCCAGTAGG GAGTGATGGA AATCCCAAAG GCTGCCACAG GCCTCCCACT 
TCTACTTCTA AGAAATGCGG GAGCAACGTT CCAAGCGCCA GCGGAGAAAT CCGTGAGCCA 
ACATTGCTTG CAGAAAATGT AGTGTTGGTT GACTGTTCTT CACTGCCTTC AGGCCGACTT 
CAGGTTGATG TCACCCTCAG GAGACAGAGT AACGCATCAG ATGACTCTCT TAGCCTTTCA 
CCAGGCACAC CCCCATCTCT GCTGAACAAT TCCACTCACA GACAAATGAT GTCAAAGCCC 
TCCACAGTGA AGCTGTCTTC TGGTATTCCA GCCAGGAAAA GAAATCACAG AGGAGAGACG 
TTACTGCACA TTGCCTCTAT AAAGGGTGAT ATATCTTCTG TTGAATACCT CTTGCAAAAT 
GGAAACGACC CAAATGTTAA GGACCATGCT GGATGGACAC CGTTGCATGA AGCCTGCAGT 
CATGGGCACC TGAAGATAGT GGAGCTGCTG CTCCAGCACA ATGCCTTGGT GAACACCACC 
GGCTATCACA ATGACTCGCC ACTGCACGAT GCCGCCAAGA ATGGCCACAT CGATATAGTC 
AAGGTGTTAC TGTCCCACGG AGCTTCCAGG AACGCTGTTA ACATATTTGG TGAGCGGCCA 
GTGGATTACA CAGACGCTGA GAATATAAGG TCATTATTGC TGCTGCCAGA GAAGACGGAT 
TCATTCTCAA CTAGCCAGTG CTCTGTCCAG GTGAACACCG GGCAGCGGAA GAGTGGGCCG 
CTGGTACTAA TAGGCAGTGG GCTTTCTTCA CAGCAGCAGA AACTGCTCAG CAAACTTGAG 
ACAGTGCTAA AGGCTAAGAA GTGTGCTGAG TTTGACAACA CAGTAACTCA TGTCATTGTT 
CCTGATGAGG AAGCTCAGAG TACCTTGAAG TGTATGCTTG GGATTCTCAA TGGATGCTGG 
GTCCTGAAGT TTGATTGGGT GAAAGCCTGT TTGGACAGCC AAGAACGTGA GCAGGAAGAA 
AAGTATGAAG TTCCTGGAGG TCCGCAGAGG AGCAGGCTCA ACAGAGAGCA GCTGCTGCCC 
AAACTGTTCG ATGGATGCTA CTTCTTTCTG GGGGGGAACT TCAAACATCA TCCAAAAGAA 
GACCTCCTGA AGCTCATTGC TGCAGCAGGA GGCAGAATCC TCAGCAGAAA GCCCAAGCCA 
GACAGTGACG TGACTCAGAC CATCAACACG GTTGCATACC ATGCCAAGCC TGACTCTGAT 
CAGCGCTTCT GTACGCAGTA CATTGTCTAT GAGGATCTGT TTAACTGTCA CCCAGAGAGG 
GTTCGGCAGG GCAAAGTCTG GATGGCTCCT TCCACCTGGC TAATCAGCTG TGTAATGGCC 
TTTGAGTTGC TTCCTCTTGA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The UBXN1 protein associates with autoubiquitinated forms of the BRCA1 tumor suppressor and inhibits its enzymatic function.
Molecular and cellular biology30(11)2787-98(2010 Jun)
Wu-Baer F,Ludwig T,Baer R

NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control.
Genes & development23(6)729-39(2009 Mar)
Wang B,Hurov K,Hofmann K,Elledge SJ

MERIT40 facilitates BRCA1 localization and DNA damage repair.
Genes & development23(6)719-28(2009 Mar)
Feng L,Huang J,Chen J

BRCA1-associated protein 1 interferes with BRCA1/BARD1 RING heterodimer activity.
Cancer research69(1)111-9(2009 Jan)
Nishikawa H,Wu W,Koike A,Kojima R,Gomi H,Fukuda M,Ohta T

The basal-like mammary carcinomas induced by Brca1 or Bard1 inactivation implicate the BRCA1/BARD1 heterodimer in tumor suppression.
Proceedings of the National Academy of Sciences of the United States of America105(19)7040-5(2008 May)
Shakya R,Szabolcs M,McCarthy E,Ospina E,Basso K,Nandula S,Murty V,Baer R,Ludwig T

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Bard1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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