Mpeg1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mpeg1
Entrez Gene ID	64552
Full Name	macrophage expressed 1
Synonyms	Mpg-1
General protein information	Preferred Names macrophage expressed 1 Names macrophage-expressed gene 1 protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	may be involved in the pathogenesis of disease related renal injury [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305460.1	NP_001292389.1	macrophage-expressed gene 1 protein precursor
XM_008760261.1	XP_008758483.1	macrophage-expressed gene 1 protein isoform X1
NM_022617.2	NP_072139.2	macrophage-expressed gene 1 protein precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MPEG1	XP_508450.1
Mus musculus (house mouse)	Mpeg1	NP_034951.1
Canis lupus familiaris (dog)	MPEG1	XP_533169.1
Homo sapiens (human)	MPEG1	NP_001034485.1
Rattus norvegicus (Norway rat)	Mpeg1	NP_072139.1
Danio rerio (zebrafish)	zgc:110354	NP_001018422.1
Danio rerio (zebrafish)	si:ch211-247l22.3	NP_001116782.1
Gallus gallus (chicken)	MPEG1	XP_003641422.1
Danio rerio (zebrafish)	mpeg1	NP_997902.1
Bos taurus (cattle)	MPEG1	NP_001039929.1

Gene Ontology
Bibliography

Related articles in PubMed

Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria.
McCormack RM, de Armas LR, Shiratsuchi M, Fiorentino DG, Olsson ML, Lichtenheld MG, Morales A, Lyapichev K, Gonzalez LE, Strbo N, Sukumar N, Stojadinovic O, Plano GV, Munson GP, Tomic-Canic M, Kirsner RS, Russell DG, Podack ER
eLife4(2015 Sep)

Perforin-2 restricts growth of Chlamydia trachomatis in macrophages.
Fields KA, McCormack R, de Armas LR, Podack ER
Infection and immunity81(8)3045-54(2013 Aug)

Inhibition of intracellular bacterial replication in fibroblasts is dependent on the perforin-like protein (perforin-2) encoded by macrophage-expressed gene 1.
McCormack R, de Armas LR, Shiratsuchi M, Ramos JE, Podack ER
Journal of innate immunity5(2)185-94(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mpeg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mpeg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa23941	NM_001305460.1 Latest version!	Rattus norvegicus macrophage expressed 1 (Mpeg1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa23941	NM_022617.2 Latest version!	Rattus norvegicus macrophage expressed 1 (Mpeg1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa23941	XM_008760261.1 Latest version!	Rattus norvegicus macrophage expressed 1 (Mpeg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mpeg1 ( NM_001305460.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001292389.1 Rattus norvegicus macrophage expressed 1 (Mpeg1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa23941
Clone ID Related Accession (Same CDS sequence)	NM_001305460.1 , XM_008760261.1 , NM_022617.2
Accession Version	NM_001305460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Rattus norvegicus(Norway rat)
Product	macrophage-expressed gene 1 protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07006310.1 and AI511268.1. On Mar 15, 2015 this sequence version replaced XM_006231115.2. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: FQ084648.1, FQ115031.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGAA ACGCCTGCAC CATGAACAGC TTCATGGCCA TTGCCCTCAT CTGGATGATG 
ATAGCATGTG CTGAAGCAGA CAAGCCTCTC AGAGACCCGG GTATGACTGG ATTTCAAACA 
TGCAAGGATA CCTTGAAGCT ACCTGTCTTG GAGGTCCTGC CAGGAGGAGG CTGGGATAAT 
CTGAGAAACA TTGACATGGG ACGGGTGATA GACTTGACCT ACACCAACTG TAAGACCACA 
GAAGATGGAC AGTACATTAT CCCTGATGAA GTCTATACTA TTCCTCAGAA GGAGAGCAAC 
CTGGAGATGA ATTCAGAAAT CCTGGATTCC TGGGTGAATT ACCAGAGTAC CACCTCATTT 
TCCATCAACA CGGAACTCTC CCTTTTCTCC AAAGTCAATG GCAAGTTTTC TACAGAGTTC 
CAAAGGATGA AGACCCTTCA AGTAAAGGAC CAAGCTGTAA CTACTAGAGT TCAGGTAAGA 
AACCGGATCT ACACAGTCAA AAACAGCCCA ACTTCAGAGC TCAGCTTCGG GTTTACGAAC 
GCACTTATGG ACATCTGTGA CCAATTAGAG AAAAACCAGA CGAAGATGGC CACCTACCTG 
GCAGAACTCT TGGTCCTCAA CTATGGCACA CATGTTATCA CCAGTGTGGA TGCTGGGGCT 
GCACTGGTTC AGGAGGATCA CATCAGGTCC TCCTTCCTCC TGGACAACCA GAACAGTGAG 
AATACTGTGA CTGCCTCTGC TGGGATTGCC TTCTTAAACA TTGTGAACTT TAAAGTTGAA 
ACAGACCACA CTTCTCAGAC CACTTTGACC AAGAGCTACC TGTCTAACAG AACCAACTCC 
AGGGTGCAGA GCTTTGGAGG GATTCCCTTC TATCCAGGAA TTACCTTAGA AACCTGGCAG 
AAGGGCATCA CTAACCACCT AGTGGCAATA GACCGAGCTG GCTTGCCTCT GCATTTCTTC 
ATTAAGCCTG ACAAACTGCC TGGTTTGCCG GGTCCCTTGG TGAAAAAGCT GTCGAAGACA 
GTGGAAACTG CTGTGAGACA TTATTACACA TTTAACACTC ACCCTGGCTG CACGAATGTC 
GACTCCCCCA ACTTCAATTT TCAAGCCAAT ATGGAAGATG ATTCGTGTGA TGCGAAAGTA 
ACCAACTTCA CCTTTGGTGG ACTTTATCAG GAATGCACTG AACTGTCAGG TGATGCTCTT 
TGCCAAAACC TGGAGCAGAA GAATCTGCTT ACTGGTGATT TCTCTTGTCC CTCTGGCTAC 
ACCCCTGTCC ATCTGCTGTC TCAGACCCAT GAGGAGGGTT ACAGTCGCCT GGAATGTAAA 
AAGAAATGCA CCCTCAAGAT TTTCTGTAAG ACAGTGTGTG AAGATGTGTT CCGAGTGGCC 
AAAGCTCAAT TCAGGGCTTA TTGGTGCGTA GCCACTGGCC AAGTACCTGA CAACTCAGGA 
CTTCTCTTTG GAGGACTCTT CACTGACAAG AGCATCAACC CAATGACAAA TGCACAGTCA 
TGCCCAGCTG GCTACATTCC ACTGAACCTG TTTGAAAGCC TTAAGGTATG TGTGTCCCTG 
GATTATGAGT TGGGGTACAA GTTTTCAGTT CCCTTTGGTG GGTTCTTCAG CTGTATAATG 
GGGAACCCCC TGGTTAATTC TGATACAGCC AAAGACATAG GAGCACCATC TCTGAAAAAA 
TGTCCTGGGG GCTTCAGCCA ACACCTAGCT GTTATCAGTG ATGGATGCCA AGTGTCCTAC 
TGTGTCAAGG CTGGAATCTT TACAGGAGGG TCCCTGCTCC CTGTCAGGCT TCCACCTTAC 
ACCAAGCCAC CCCTCATGAG CCAAGTTGCC ACCAACACTG TCATAGTGAC CAGTAGTGAG 
ACTGCCAGAT CCTGGATTAA GGATCCTCAG ACCAACCAGT GGAAGCTGGG AGAGCCTCTG 
GAGCTTCATA GAGCCATGAC TGTCATCCAT GGGGACGGTA ATGGAATGTC AGGAGGGGAA 
GCTGCTGGAG TCACGTTGGG AGTCATCATA GCACTAGGAA TTGTCATTAC CTTGGCCATC 
TACAGTACTC GGAAGTACAA GAAAGAGAAG GAATACCAGG AAATCGAGGA GCAGGAGAGT 
TTGGTTGGAA GCTTTGCAAC AGATGCATCA GCCCCTAATG GAGAACAGGA TCCATGTCCA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292389.1
CDS	149..2314
Translation	MAGNACTMNSFMAIALIWMMIACAEADKPLRDPGMTGFQTCKDTLKLPVLEVLPGGGWDNLRNIDMGRVIDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEILDSWVNYQSTTSFSINTELSLFSKVNGKFSTEFQRMKTLQVKDQAVTTRVQVRNRIYTVKNSPTSELSFGFTNALMDICDQLEKNQTKMATYLAELLVLNYGTHVITSVDAGAALVQEDHIRSSFLLDNQNSENTVTASAGIAFLNIVNFKVETDHTSQTTLTKSYLSNRTNSRVQSFGGIPFYPGITLETWQKGITNHLVAIDRAGLPLHFFIKPDKLPGLPGPLVKKLSKTVETAVRHYYTFNTHPGCTNVDSPNFNFQANMEDDSCDAKVTNFTFGGLYQECTELSGDALCQNLEQKNLLTGDFSCPSGYTPVHLLSQTHEEGYSRLECKKKCTLKIFCKTVCEDVFRVAKAQFRAYWCVATGQVPDNSGLLFGGLFTDKSINPMTNAQSCPAGYIPLNLFESLKVCVSLDYELGYKFSVPFGGFFSCIMGNPLVNSDTAKDIGAPSLKKCPGGFSQHLAVISDGCQVSYCVKAGIFTGGSLLPVRLPPYTKPPLMSQVATNTVIVTSSETARSWIKDPQTNQWKLGEPLELHRAMTVIHGDGNGMSGGEAAGVTLGVIIALGIVITLAIYSTRKYKKEKEYQEIEEQESLVGSFATDASAPNGEQDPCPA

Target ORF information:

RefSeq Version	NM_001305460.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus macrophage expressed 1 (Mpeg1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305460.1

ORF Insert Sequence:

ATGGCTGGAA ACGCCTGCAC CATGAACAGC TTCATGGCCA TTGCCCTCAT CTGGATGATG 
ATAGCATGTG CTGAAGCAGA CAAGCCTCTC AGAGACCCGG GTATGACTGG ATTTCAAACA 
TGCAAGGATA CCTTGAAGCT ACCTGTCTTG GAGGTCCTGC CAGGAGGAGG CTGGGATAAT 
CTGAGAAACA TTGACATGGG ACGGGTGATA GACTTGACCT ACACCAACTG TAAGACCACA 
GAAGATGGAC AGTACATTAT CCCTGATGAA GTCTATACTA TTCCTCAGAA GGAGAGCAAC 
CTGGAGATGA ATTCAGAAAT CCTGGATTCC TGGGTGAATT ACCAGAGTAC CACCTCATTT 
TCCATCAACA CGGAACTCTC CCTTTTCTCC AAAGTCAATG GCAAGTTTTC TACAGAGTTC 
CAAAGGATGA AGACCCTTCA AGTAAAGGAC CAAGCTGTAA CTACTAGAGT TCAGGTAAGA 
AACCGGATCT ACACAGTCAA AAACAGCCCA ACTTCAGAGC TCAGCTTCGG GTTTACGAAC 
GCACTTATGG ACATCTGTGA CCAATTAGAG AAAAACCAGA CGAAGATGGC CACCTACCTG 
GCAGAACTCT TGGTCCTCAA CTATGGCACA CATGTTATCA CCAGTGTGGA TGCTGGGGCT 
GCACTGGTTC AGGAGGATCA CATCAGGTCC TCCTTCCTCC TGGACAACCA GAACAGTGAG 
AATACTGTGA CTGCCTCTGC TGGGATTGCC TTCTTAAACA TTGTGAACTT TAAAGTTGAA 
ACAGACCACA CTTCTCAGAC CACTTTGACC AAGAGCTACC TGTCTAACAG AACCAACTCC 
AGGGTGCAGA GCTTTGGAGG GATTCCCTTC TATCCAGGAA TTACCTTAGA AACCTGGCAG 
AAGGGCATCA CTAACCACCT AGTGGCAATA GACCGAGCTG GCTTGCCTCT GCATTTCTTC 
ATTAAGCCTG ACAAACTGCC TGGTTTGCCG GGTCCCTTGG TGAAAAAGCT GTCGAAGACA 
GTGGAAACTG CTGTGAGACA TTATTACACA TTTAACACTC ACCCTGGCTG CACGAATGTC 
GACTCCCCCA ACTTCAATTT TCAAGCCAAT ATGGAAGATG ATTCGTGTGA TGCGAAAGTA 
ACCAACTTCA CCTTTGGTGG ACTTTATCAG GAATGCACTG AACTGTCAGG TGATGCTCTT 
TGCCAAAACC TGGAGCAGAA GAATCTGCTT ACTGGTGATT TCTCTTGTCC CTCTGGCTAC 
ACCCCTGTCC ATCTGCTGTC TCAGACCCAT GAGGAGGGTT ACAGTCGCCT GGAATGTAAA 
AAGAAATGCA CCCTCAAGAT TTTCTGTAAG ACAGTGTGTG AAGATGTGTT CCGAGTGGCC 
AAAGCTCAAT TCAGGGCTTA TTGGTGCGTA GCCACTGGCC AAGTACCTGA CAACTCAGGA 
CTTCTCTTTG GAGGACTCTT CACTGACAAG AGCATCAACC CAATGACAAA TGCACAGTCA 
TGCCCAGCTG GCTACATTCC ACTGAACCTG TTTGAAAGCC TTAAGGTATG TGTGTCCCTG 
GATTATGAGT TGGGGTACAA GTTTTCAGTT CCCTTTGGTG GGTTCTTCAG CTGTATAATG 
GGGAACCCCC TGGTTAATTC TGATACAGCC AAAGACATAG GAGCACCATC TCTGAAAAAA 
TGTCCTGGGG GCTTCAGCCA ACACCTAGCT GTTATCAGTG ATGGATGCCA AGTGTCCTAC 
TGTGTCAAGG CTGGAATCTT TACAGGAGGG TCCCTGCTCC CTGTCAGGCT TCCACCTTAC 
ACCAAGCCAC CCCTCATGAG CCAAGTTGCC ACCAACACTG TCATAGTGAC CAGTAGTGAG 
ACTGCCAGAT CCTGGATTAA GGATCCTCAG ACCAACCAGT GGAAGCTGGG AGAGCCTCTG 
GAGCTTCATA GAGCCATGAC TGTCATCCAT GGGGACGGTA ATGGAATGTC AGGAGGGGAA 
GCTGCTGGAG TCACGTTGGG AGTCATCATA GCACTAGGAA TTGTCATTAC CTTGGCCATC 
TACAGTACTC GGAAGTACAA GAAAGAGAAG GAATACCAGG AAATCGAGGA GCAGGAGAGT 
TTGGTTGGAA GCTTTGCAAC AGATGCATCA GCCCCTAATG GAGAACAGGA TCCATGTCCA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mpeg1 ( NM_022617.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_072139.2 Rattus norvegicus macrophage expressed 1 (Mpeg1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa23941
Clone ID Related Accession (Same CDS sequence)	NM_001305460.1 , XM_008760261.1 , NM_022617.2
Accession Version	NM_022617.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Rattus norvegicus(Norway rat)
Product	macrophage-expressed gene 1 protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07006310.1 and AI511268.1. On Mar 15, 2015 this sequence version replaced NM_022617.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. COMPLETENESS: complete on the 3' end.

ATGGCTGGAA ACGCCTGCAC CATGAACAGC TTCATGGCCA TTGCCCTCAT CTGGATGATG 
ATAGCATGTG CTGAAGCAGA CAAGCCTCTC AGAGACCCGG GTATGACTGG ATTTCAAACA 
TGCAAGGATA CCTTGAAGCT ACCTGTCTTG GAGGTCCTGC CAGGAGGAGG CTGGGATAAT 
CTGAGAAACA TTGACATGGG ACGGGTGATA GACTTGACCT ACACCAACTG TAAGACCACA 
GAAGATGGAC AGTACATTAT CCCTGATGAA GTCTATACTA TTCCTCAGAA GGAGAGCAAC 
CTGGAGATGA ATTCAGAAAT CCTGGATTCC TGGGTGAATT ACCAGAGTAC CACCTCATTT 
TCCATCAACA CGGAACTCTC CCTTTTCTCC AAAGTCAATG GCAAGTTTTC TACAGAGTTC 
CAAAGGATGA AGACCCTTCA AGTAAAGGAC CAAGCTGTAA CTACTAGAGT TCAGGTAAGA 
AACCGGATCT ACACAGTCAA AAACAGCCCA ACTTCAGAGC TCAGCTTCGG GTTTACGAAC 
GCACTTATGG ACATCTGTGA CCAATTAGAG AAAAACCAGA CGAAGATGGC CACCTACCTG 
GCAGAACTCT TGGTCCTCAA CTATGGCACA CATGTTATCA CCAGTGTGGA TGCTGGGGCT 
GCACTGGTTC AGGAGGATCA CATCAGGTCC TCCTTCCTCC TGGACAACCA GAACAGTGAG 
AATACTGTGA CTGCCTCTGC TGGGATTGCC TTCTTAAACA TTGTGAACTT TAAAGTTGAA 
ACAGACCACA CTTCTCAGAC CACTTTGACC AAGAGCTACC TGTCTAACAG AACCAACTCC 
AGGGTGCAGA GCTTTGGAGG GATTCCCTTC TATCCAGGAA TTACCTTAGA AACCTGGCAG 
AAGGGCATCA CTAACCACCT AGTGGCAATA GACCGAGCTG GCTTGCCTCT GCATTTCTTC 
ATTAAGCCTG ACAAACTGCC TGGTTTGCCG GGTCCCTTGG TGAAAAAGCT GTCGAAGACA 
GTGGAAACTG CTGTGAGACA TTATTACACA TTTAACACTC ACCCTGGCTG CACGAATGTC 
GACTCCCCCA ACTTCAATTT TCAAGCCAAT ATGGAAGATG ATTCGTGTGA TGCGAAAGTA 
ACCAACTTCA CCTTTGGTGG ACTTTATCAG GAATGCACTG AACTGTCAGG TGATGCTCTT 
TGCCAAAACC TGGAGCAGAA GAATCTGCTT ACTGGTGATT TCTCTTGTCC CTCTGGCTAC 
ACCCCTGTCC ATCTGCTGTC TCAGACCCAT GAGGAGGGTT ACAGTCGCCT GGAATGTAAA 
AAGAAATGCA CCCTCAAGAT TTTCTGTAAG ACAGTGTGTG AAGATGTGTT CCGAGTGGCC 
AAAGCTCAAT TCAGGGCTTA TTGGTGCGTA GCCACTGGCC AAGTACCTGA CAACTCAGGA 
CTTCTCTTTG GAGGACTCTT CACTGACAAG AGCATCAACC CAATGACAAA TGCACAGTCA 
TGCCCAGCTG GCTACATTCC ACTGAACCTG TTTGAAAGCC TTAAGGTATG TGTGTCCCTG 
GATTATGAGT TGGGGTACAA GTTTTCAGTT CCCTTTGGTG GGTTCTTCAG CTGTATAATG 
GGGAACCCCC TGGTTAATTC TGATACAGCC AAAGACATAG GAGCACCATC TCTGAAAAAA 
TGTCCTGGGG GCTTCAGCCA ACACCTAGCT GTTATCAGTG ATGGATGCCA AGTGTCCTAC 
TGTGTCAAGG CTGGAATCTT TACAGGAGGG TCCCTGCTCC CTGTCAGGCT TCCACCTTAC 
ACCAAGCCAC CCCTCATGAG CCAAGTTGCC ACCAACACTG TCATAGTGAC CAGTAGTGAG 
ACTGCCAGAT CCTGGATTAA GGATCCTCAG ACCAACCAGT GGAAGCTGGG AGAGCCTCTG 
GAGCTTCATA GAGCCATGAC TGTCATCCAT GGGGACGGTA ATGGAATGTC AGGAGGGGAA 
GCTGCTGGAG TCACGTTGGG AGTCATCATA GCACTAGGAA TTGTCATTAC CTTGGCCATC 
TACAGTACTC GGAAGTACAA GAAAGAGAAG GAATACCAGG AAATCGAGGA GCAGGAGAGT 
TTGGTTGGAA GCTTTGCAAC AGATGCATCA GCCCCTAATG GAGAACAGGA TCCATGTCCA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072139.2
CDS	115..2280
Translation	MAGNACTMNSFMAIALIWMMIACAEADKPLRDPGMTGFQTCKDTLKLPVLEVLPGGGWDNLRNIDMGRVIDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEILDSWVNYQSTTSFSINTELSLFSKVNGKFSTEFQRMKTLQVKDQAVTTRVQVRNRIYTVKNSPTSELSFGFTNALMDICDQLEKNQTKMATYLAELLVLNYGTHVITSVDAGAALVQEDHIRSSFLLDNQNSENTVTASAGIAFLNIVNFKVETDHTSQTTLTKSYLSNRTNSRVQSFGGIPFYPGITLETWQKGITNHLVAIDRAGLPLHFFIKPDKLPGLPGPLVKKLSKTVETAVRHYYTFNTHPGCTNVDSPNFNFQANMEDDSCDAKVTNFTFGGLYQECTELSGDALCQNLEQKNLLTGDFSCPSGYTPVHLLSQTHEEGYSRLECKKKCTLKIFCKTVCEDVFRVAKAQFRAYWCVATGQVPDNSGLLFGGLFTDKSINPMTNAQSCPAGYIPLNLFESLKVCVSLDYELGYKFSVPFGGFFSCIMGNPLVNSDTAKDIGAPSLKKCPGGFSQHLAVISDGCQVSYCVKAGIFTGGSLLPVRLPPYTKPPLMSQVATNTVIVTSSETARSWIKDPQTNQWKLGEPLELHRAMTVIHGDGNGMSGGEAAGVTLGVIIALGIVITLAIYSTRKYKKEKEYQEIEEQESLVGSFATDASAPNGEQDPCPA

Target ORF information:

RefSeq Version	NM_022617.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus macrophage expressed 1 (Mpeg1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022617.2

ORF Insert Sequence:

ATGGCTGGAA ACGCCTGCAC CATGAACAGC TTCATGGCCA TTGCCCTCAT CTGGATGATG 
ATAGCATGTG CTGAAGCAGA CAAGCCTCTC AGAGACCCGG GTATGACTGG ATTTCAAACA 
TGCAAGGATA CCTTGAAGCT ACCTGTCTTG GAGGTCCTGC CAGGAGGAGG CTGGGATAAT 
CTGAGAAACA TTGACATGGG ACGGGTGATA GACTTGACCT ACACCAACTG TAAGACCACA 
GAAGATGGAC AGTACATTAT CCCTGATGAA GTCTATACTA TTCCTCAGAA GGAGAGCAAC 
CTGGAGATGA ATTCAGAAAT CCTGGATTCC TGGGTGAATT ACCAGAGTAC CACCTCATTT 
TCCATCAACA CGGAACTCTC CCTTTTCTCC AAAGTCAATG GCAAGTTTTC TACAGAGTTC 
CAAAGGATGA AGACCCTTCA AGTAAAGGAC CAAGCTGTAA CTACTAGAGT TCAGGTAAGA 
AACCGGATCT ACACAGTCAA AAACAGCCCA ACTTCAGAGC TCAGCTTCGG GTTTACGAAC 
GCACTTATGG ACATCTGTGA CCAATTAGAG AAAAACCAGA CGAAGATGGC CACCTACCTG 
GCAGAACTCT TGGTCCTCAA CTATGGCACA CATGTTATCA CCAGTGTGGA TGCTGGGGCT 
GCACTGGTTC AGGAGGATCA CATCAGGTCC TCCTTCCTCC TGGACAACCA GAACAGTGAG 
AATACTGTGA CTGCCTCTGC TGGGATTGCC TTCTTAAACA TTGTGAACTT TAAAGTTGAA 
ACAGACCACA CTTCTCAGAC CACTTTGACC AAGAGCTACC TGTCTAACAG AACCAACTCC 
AGGGTGCAGA GCTTTGGAGG GATTCCCTTC TATCCAGGAA TTACCTTAGA AACCTGGCAG 
AAGGGCATCA CTAACCACCT AGTGGCAATA GACCGAGCTG GCTTGCCTCT GCATTTCTTC 
ATTAAGCCTG ACAAACTGCC TGGTTTGCCG GGTCCCTTGG TGAAAAAGCT GTCGAAGACA 
GTGGAAACTG CTGTGAGACA TTATTACACA TTTAACACTC ACCCTGGCTG CACGAATGTC 
GACTCCCCCA ACTTCAATTT TCAAGCCAAT ATGGAAGATG ATTCGTGTGA TGCGAAAGTA 
ACCAACTTCA CCTTTGGTGG ACTTTATCAG GAATGCACTG AACTGTCAGG TGATGCTCTT 
TGCCAAAACC TGGAGCAGAA GAATCTGCTT ACTGGTGATT TCTCTTGTCC CTCTGGCTAC 
ACCCCTGTCC ATCTGCTGTC TCAGACCCAT GAGGAGGGTT ACAGTCGCCT GGAATGTAAA 
AAGAAATGCA CCCTCAAGAT TTTCTGTAAG ACAGTGTGTG AAGATGTGTT CCGAGTGGCC 
AAAGCTCAAT TCAGGGCTTA TTGGTGCGTA GCCACTGGCC AAGTACCTGA CAACTCAGGA 
CTTCTCTTTG GAGGACTCTT CACTGACAAG AGCATCAACC CAATGACAAA TGCACAGTCA 
TGCCCAGCTG GCTACATTCC ACTGAACCTG TTTGAAAGCC TTAAGGTATG TGTGTCCCTG 
GATTATGAGT TGGGGTACAA GTTTTCAGTT CCCTTTGGTG GGTTCTTCAG CTGTATAATG 
GGGAACCCCC TGGTTAATTC TGATACAGCC AAAGACATAG GAGCACCATC TCTGAAAAAA 
TGTCCTGGGG GCTTCAGCCA ACACCTAGCT GTTATCAGTG ATGGATGCCA AGTGTCCTAC 
TGTGTCAAGG CTGGAATCTT TACAGGAGGG TCCCTGCTCC CTGTCAGGCT TCCACCTTAC 
ACCAAGCCAC CCCTCATGAG CCAAGTTGCC ACCAACACTG TCATAGTGAC CAGTAGTGAG 
ACTGCCAGAT CCTGGATTAA GGATCCTCAG ACCAACCAGT GGAAGCTGGG AGAGCCTCTG 
GAGCTTCATA GAGCCATGAC TGTCATCCAT GGGGACGGTA ATGGAATGTC AGGAGGGGAA 
GCTGCTGGAG TCACGTTGGG AGTCATCATA GCACTAGGAA TTGTCATTAC CTTGGCCATC 
TACAGTACTC GGAAGTACAA GAAAGAGAAG GAATACCAGG AAATCGAGGA GCAGGAGAGT 
TTGGTTGGAA GCTTTGCAAC AGATGCATCA GCCCCTAATG GAGAACAGGA TCCATGTCCA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mpeg1 ( XM_008760261.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758483.1 Rattus norvegicus macrophage expressed 1 (Mpeg1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23941
Clone ID Related Accession (Same CDS sequence)	NM_001305460.1 , XM_008760261.1 , NM_022617.2
Accession Version	XM_008760261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	macrophage-expressed gene 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA ACGCCTGCAC CATGAACAGC TTCATGGCCA TTGCCCTCAT CTGGATGATG 
ATAGCATGTG CTGAAGCAGA CAAGCCTCTC AGAGACCCGG GTATGACTGG ATTTCAAACA 
TGCAAGGATA CCTTGAAGCT ACCTGTCTTG GAGGTCCTGC CAGGAGGAGG CTGGGATAAT 
CTGAGAAACA TTGACATGGG ACGGGTGATA GACTTGACCT ACACCAACTG TAAGACCACA 
GAAGATGGAC AGTACATTAT CCCTGATGAA GTCTATACTA TTCCTCAGAA GGAGAGCAAC 
CTGGAGATGA ATTCAGAAAT CCTGGATTCC TGGGTGAATT ACCAGAGTAC CACCTCATTT 
TCCATCAACA CGGAACTCTC CCTTTTCTCC AAAGTCAATG GCAAGTTTTC TACAGAGTTC 
CAAAGGATGA AGACCCTTCA AGTAAAGGAC CAAGCTGTAA CTACTAGAGT TCAGGTAAGA 
AACCGGATCT ACACAGTCAA AAACAGCCCA ACTTCAGAGC TCAGCTTCGG GTTTACGAAC 
GCACTTATGG ACATCTGTGA CCAATTAGAG AAAAACCAGA CGAAGATGGC CACCTACCTG 
GCAGAACTCT TGGTCCTCAA CTATGGCACA CATGTTATCA CCAGTGTGGA TGCTGGGGCT 
GCACTGGTTC AGGAGGATCA CATCAGGTCC TCCTTCCTCC TGGACAACCA GAACAGTGAG 
AATACTGTGA CTGCCTCTGC TGGGATTGCC TTCTTAAACA TTGTGAACTT TAAAGTTGAA 
ACAGACCACA CTTCTCAGAC CACTTTGACC AAGAGCTACC TGTCTAACAG AACCAACTCC 
AGGGTGCAGA GCTTTGGAGG GATTCCCTTC TATCCAGGAA TTACCTTAGA AACCTGGCAG 
AAGGGCATCA CTAACCACCT AGTGGCAATA GACCGAGCTG GCTTGCCTCT GCATTTCTTC 
ATTAAGCCTG ACAAACTGCC TGGTTTGCCG GGTCCCTTGG TGAAAAAGCT GTCGAAGACA 
GTGGAAACTG CTGTGAGACA TTATTACACA TTTAACACTC ACCCTGGCTG CACGAATGTC 
GACTCCCCCA ACTTCAATTT TCAAGCCAAT ATGGAAGATG ATTCGTGTGA TGCGAAAGTA 
ACCAACTTCA CCTTTGGTGG ACTTTATCAG GAATGCACTG AACTGTCAGG TGATGCTCTT 
TGCCAAAACC TGGAGCAGAA GAATCTGCTT ACTGGTGATT TCTCTTGTCC CTCTGGCTAC 
ACCCCTGTCC ATCTGCTGTC TCAGACCCAT GAGGAGGGTT ACAGTCGCCT GGAATGTAAA 
AAGAAATGCA CCCTCAAGAT TTTCTGTAAG ACAGTGTGTG AAGATGTGTT CCGAGTGGCC 
AAAGCTCAAT TCAGGGCTTA TTGGTGCGTA GCCACTGGCC AAGTACCTGA CAACTCAGGA 
CTTCTCTTTG GAGGACTCTT CACTGACAAG AGCATCAACC CAATGACAAA TGCACAGTCA 
TGCCCAGCTG GCTACATTCC ACTGAACCTG TTTGAAAGCC TTAAGGTATG TGTGTCCCTG 
GATTATGAGT TGGGGTACAA GTTTTCAGTT CCCTTTGGTG GGTTCTTCAG CTGTATAATG 
GGGAACCCCC TGGTTAATTC TGATACAGCC AAAGACATAG GAGCACCATC TCTGAAAAAA 
TGTCCTGGGG GCTTCAGCCA ACACCTAGCT GTTATCAGTG ATGGATGCCA AGTGTCCTAC 
TGTGTCAAGG CTGGAATCTT TACAGGAGGG TCCCTGCTCC CTGTCAGGCT TCCACCTTAC 
ACCAAGCCAC CCCTCATGAG CCAAGTTGCC ACCAACACTG TCATAGTGAC CAGTAGTGAG 
ACTGCCAGAT CCTGGATTAA GGATCCTCAG ACCAACCAGT GGAAGCTGGG AGAGCCTCTG 
GAGCTTCATA GAGCCATGAC TGTCATCCAT GGGGACGGTA ATGGAATGTC AGGAGGGGAA 
GCTGCTGGAG TCACGTTGGG AGTCATCATA GCACTAGGAA TTGTCATTAC CTTGGCCATC 
TACAGTACTC GGAAGTACAA GAAAGAGAAG GAATACCAGG AAATCGAGGA GCAGGAGAGT 
TTGGTTGGAA GCTTTGCAAC AGATGCATCA GCCCCTAATG GAGAACAGGA TCCATGTCCA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758483.1
CDS	266..2431
Translation	MAGNACTMNSFMAIALIWMMIACAEADKPLRDPGMTGFQTCKDTLKLPVLEVLPGGGWDNLRNIDMGRVIDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEILDSWVNYQSTTSFSINTELSLFSKVNGKFSTEFQRMKTLQVKDQAVTTRVQVRNRIYTVKNSPTSELSFGFTNALMDICDQLEKNQTKMATYLAELLVLNYGTHVITSVDAGAALVQEDHIRSSFLLDNQNSENTVTASAGIAFLNIVNFKVETDHTSQTTLTKSYLSNRTNSRVQSFGGIPFYPGITLETWQKGITNHLVAIDRAGLPLHFFIKPDKLPGLPGPLVKKLSKTVETAVRHYYTFNTHPGCTNVDSPNFNFQANMEDDSCDAKVTNFTFGGLYQECTELSGDALCQNLEQKNLLTGDFSCPSGYTPVHLLSQTHEEGYSRLECKKKCTLKIFCKTVCEDVFRVAKAQFRAYWCVATGQVPDNSGLLFGGLFTDKSINPMTNAQSCPAGYIPLNLFESLKVCVSLDYELGYKFSVPFGGFFSCIMGNPLVNSDTAKDIGAPSLKKCPGGFSQHLAVISDGCQVSYCVKAGIFTGGSLLPVRLPPYTKPPLMSQVATNTVIVTSSETARSWIKDPQTNQWKLGEPLELHRAMTVIHGDGNGMSGGEAAGVTLGVIIALGIVITLAIYSTRKYKKEKEYQEIEEQESLVGSFATDASAPNGEQDPCPA

Target ORF information:

RefSeq Version	XM_008760261.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus macrophage expressed 1 (Mpeg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760261.1

ORF Insert Sequence:

ATGGCTGGAA ACGCCTGCAC CATGAACAGC TTCATGGCCA TTGCCCTCAT CTGGATGATG 
ATAGCATGTG CTGAAGCAGA CAAGCCTCTC AGAGACCCGG GTATGACTGG ATTTCAAACA 
TGCAAGGATA CCTTGAAGCT ACCTGTCTTG GAGGTCCTGC CAGGAGGAGG CTGGGATAAT 
CTGAGAAACA TTGACATGGG ACGGGTGATA GACTTGACCT ACACCAACTG TAAGACCACA 
GAAGATGGAC AGTACATTAT CCCTGATGAA GTCTATACTA TTCCTCAGAA GGAGAGCAAC 
CTGGAGATGA ATTCAGAAAT CCTGGATTCC TGGGTGAATT ACCAGAGTAC CACCTCATTT 
TCCATCAACA CGGAACTCTC CCTTTTCTCC AAAGTCAATG GCAAGTTTTC TACAGAGTTC 
CAAAGGATGA AGACCCTTCA AGTAAAGGAC CAAGCTGTAA CTACTAGAGT TCAGGTAAGA 
AACCGGATCT ACACAGTCAA AAACAGCCCA ACTTCAGAGC TCAGCTTCGG GTTTACGAAC 
GCACTTATGG ACATCTGTGA CCAATTAGAG AAAAACCAGA CGAAGATGGC CACCTACCTG 
GCAGAACTCT TGGTCCTCAA CTATGGCACA CATGTTATCA CCAGTGTGGA TGCTGGGGCT 
GCACTGGTTC AGGAGGATCA CATCAGGTCC TCCTTCCTCC TGGACAACCA GAACAGTGAG 
AATACTGTGA CTGCCTCTGC TGGGATTGCC TTCTTAAACA TTGTGAACTT TAAAGTTGAA 
ACAGACCACA CTTCTCAGAC CACTTTGACC AAGAGCTACC TGTCTAACAG AACCAACTCC 
AGGGTGCAGA GCTTTGGAGG GATTCCCTTC TATCCAGGAA TTACCTTAGA AACCTGGCAG 
AAGGGCATCA CTAACCACCT AGTGGCAATA GACCGAGCTG GCTTGCCTCT GCATTTCTTC 
ATTAAGCCTG ACAAACTGCC TGGTTTGCCG GGTCCCTTGG TGAAAAAGCT GTCGAAGACA 
GTGGAAACTG CTGTGAGACA TTATTACACA TTTAACACTC ACCCTGGCTG CACGAATGTC 
GACTCCCCCA ACTTCAATTT TCAAGCCAAT ATGGAAGATG ATTCGTGTGA TGCGAAAGTA 
ACCAACTTCA CCTTTGGTGG ACTTTATCAG GAATGCACTG AACTGTCAGG TGATGCTCTT 
TGCCAAAACC TGGAGCAGAA GAATCTGCTT ACTGGTGATT TCTCTTGTCC CTCTGGCTAC 
ACCCCTGTCC ATCTGCTGTC TCAGACCCAT GAGGAGGGTT ACAGTCGCCT GGAATGTAAA 
AAGAAATGCA CCCTCAAGAT TTTCTGTAAG ACAGTGTGTG AAGATGTGTT CCGAGTGGCC 
AAAGCTCAAT TCAGGGCTTA TTGGTGCGTA GCCACTGGCC AAGTACCTGA CAACTCAGGA 
CTTCTCTTTG GAGGACTCTT CACTGACAAG AGCATCAACC CAATGACAAA TGCACAGTCA 
TGCCCAGCTG GCTACATTCC ACTGAACCTG TTTGAAAGCC TTAAGGTATG TGTGTCCCTG 
GATTATGAGT TGGGGTACAA GTTTTCAGTT CCCTTTGGTG GGTTCTTCAG CTGTATAATG 
GGGAACCCCC TGGTTAATTC TGATACAGCC AAAGACATAG GAGCACCATC TCTGAAAAAA 
TGTCCTGGGG GCTTCAGCCA ACACCTAGCT GTTATCAGTG ATGGATGCCA AGTGTCCTAC 
TGTGTCAAGG CTGGAATCTT TACAGGAGGG TCCCTGCTCC CTGTCAGGCT TCCACCTTAC 
ACCAAGCCAC CCCTCATGAG CCAAGTTGCC ACCAACACTG TCATAGTGAC CAGTAGTGAG 
ACTGCCAGAT CCTGGATTAA GGATCCTCAG ACCAACCAGT GGAAGCTGGG AGAGCCTCTG 
GAGCTTCATA GAGCCATGAC TGTCATCCAT GGGGACGGTA ATGGAATGTC AGGAGGGGAA 
GCTGCTGGAG TCACGTTGGG AGTCATCATA GCACTAGGAA TTGTCATTAC CTTGGCCATC 
TACAGTACTC GGAAGTACAA GAAAGAGAAG GAATACCAGG AAATCGAGGA GCAGGAGAGT 
TTGGTTGGAA GCTTTGCAAC AGATGCATCA GCCCCTAATG GAGAACAGGA TCCATGTCCA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria.
eLife4(2015 Sep)
McCormack RM,de Armas LR,Shiratsuchi M,Fiorentino DG,Olsson ML,Lichtenheld MG,Morales A,Lyapichev K,Gonzalez LE,Strbo N,Sukumar N,Stojadinovic O,Plano GV,Munson GP,Tomic-Canic M,Kirsner RS,Russell DG,Podack ER

Perforin-2 restricts growth of Chlamydia trachomatis in macrophages.
Infection and immunity81(8)3045-54(2013 Aug)
Fields KA,McCormack R,de Armas LR,Podack ER

Inhibition of intracellular bacterial replication in fibroblasts is dependent on the perforin-like protein (perforin-2) encoded by macrophage-expressed gene 1.
Journal of innate immunity5(2)185-94(2013)
McCormack R,de Armas LR,Shiratsuchi M,Ramos JE,Podack ER

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Mpeg1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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