Thop1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Thop1
Entrez Gene ID	64517
Full Name	thimet oligopeptidase 1
Synonyms	EP24.15
General protein information	Preferred Names thimet oligopeptidase 1 Names thimet oligopeptidase PZ-peptidase endo-oligopeptidase A endopeptidase 24.15 metalloendopeptidase soluble metallo-endopeptidase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	metallopeptidase that catalyzes the thiol-dependent cleavage of many neuropeptides [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_172075.2	NP_742072.2	thimet oligopeptidase
XM_006240995.3	XP_006241057.1	thimet oligopeptidase isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	THOP1	NP_001029163.2
Eremothecium gossypii	AGOS_AGR405C	NP_987071.2
Mus musculus (house mouse)	Thop1	NP_073144.3
Gallus gallus (chicken)	LOC426398	XP_004948943.1
Xenopus tropicalis (tropical clawed frog)	thop1	NP_001072409.1
Homo sapiens (human)	THOP1	NP_003240.1
Canis lupus familiaris (dog)	THOP1	XP_533954.3
Eremothecium gossypii	AGOS_AGR406C	NP_987072.1
Oryza sativa (rice)	Os01g0902200	NP_001045116.1
Rattus norvegicus (Norway rat)	Thop1	NP_742072.2
Danio rerio (zebrafish)	zgc:92139	NP_001002355.1
Arabidopsis thaliana (thale cress)	AT1G67690	NP_564902.4
Pan troglodytes (chimpanzee)	THOP1	XP_003953368.1
	THOP1	XP_001117760.2

Gene Ontology
Bibliography

Related articles in PubMed

EP24.15 as a Potential Regulator of Kisspeptin Within the Neuroendocrine Hypothalamus.
Woitowich NC, Philibert KD, Leitermann RJ, Wungjiranirun M, Urban JH, Glucksman MJ
Endocrinology157(2)820-30(2016 Feb)

Angiotensin II-independent angiotensin-(1-7) formation in rat hippocampus: involvement of thimet oligopeptidase.
Pereira MG, Souza LL, Becari C, Duarte DA, Camacho FR, Oliveira JA, Gomes MD, Oliveira EB, Salgado MC, Garcia-Cairasco N, Costa-Neto CM
Hypertension (Dallas, Tex. : 1979)62(5)879-85(2013 Nov)

Natural intracellular peptides can modulate the interactions of mouse brain proteins and thimet oligopeptidase with 14-3-3? and calmodulin.
Russo LC, Asega AF, Castro LM, Negraes PD, Cruz L, Gozzo FC, Ulrich H, Camargo AC, Rioli V, Ferro ES
Proteomics12(17)2641-55(2012 Aug)

Acute cocaine treatment increases thimet oligopeptidase in the striatum of rat brain.
Dalio FM, Visniauskas B, Bicocchi ES, Perry JC, Freua R, Gesteira TF, Nader HB, Machado MF, Tufik S, Ferro ES, Andersen ML, Toledo CA, Chagas JR, Oliveira V
Biochemical and biophysical research communications419(4)724-7(2012 Mar)

Increased expression of the oligopeptidase THOP1 is a neuroprotective response to Abeta toxicity.
Pollio G, Hoozemans JJ, Andersen CA, Roncarati R, Rosi MC, van Haastert ES, Seredenina T, Diamanti D, Gotta S, Fiorentini A, Magnoni L, Raggiaschi R, Rozemuller AJ, Casamenti F, Caricasole A, Terstappen GC
Neurobiology of disease31(1)145-58(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Coincubation of Kiss1 and EP24.15 demonstrated multiple cleavages of the peptide in vitro. immunohistochemistry indicated coexpression of EP24.15 and Kiss1, implicating that the regulation of Kiss1 by EP24.15 could occur in vivo.
Title: EP24.15 as a Potential Regulator of Kisspeptin Within the Neuroendocrine Hypothalamus.

we show that Ang-(1-7) is the main metabolite of angiotensin I in rat hippocampi, and, strikingly, that thimet oligopeptidase is the main enzyme involved in the generation of Ang-(1-7).
Title: Angiotensin II-independent angiotensin-(1-7) formation in rat hippocampus: involvement of thimet oligopeptidase.

TOP changes in the brain could play important role in the balance of neuropeptide level correlated with cocaine effects.
Title: Acute cocaine treatment increases thimet oligopeptidase in the striatum of rat brain.

increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load
Title: Increased expression of the oligopeptidase THOP1 is a neuroprotective response to Abeta toxicity.

together, these data demonstrate that EP24.15/16 modulate intracellular, peptidergic signaling cascades through B2R in a physiologically relevant nociceptive system.
Title: Modulation of bradykinin signaling by EP24.15 and EP24.16 in cultured trigeminal ganglia.

The following Thop1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Thop1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06048	NM_172075.2 Latest version!	Rattus norvegicus thimet oligopeptidase 1 (Thop1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa29906	XM_006240995.3 Latest version!	Rattus norvegicus thimet oligopeptidase 1 (Thop1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Thop1 ( NM_172075.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742072.2 Rattus norvegicus thimet oligopeptidase 1 (Thop1), mRNA.

Price & Availability↑

CloneID	ORa06048
Clone ID Related Accession (Same CDS sequence)	NM_172075.2
Accession Version	NM_172075.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	thimet oligopeptidase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB780843.1 and BC081706.1. On Mar 8, 2007 this sequence version replaced NM_172075.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081706.1, M61142.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCCCC CCGCAGCCTG TGCTGGGGAC GTGGTGGATA CAGTGTCACC ATGCTCCACC 
GTGAATCATC TGCGCTGGGA CCTGAGCGCA CAGCAGATCA GAGCTCTAAC CACACAGCTC 
ATCGAGCAGA CCAAGTGTGT GTACGACCGT GTGGGCGCCC AGGACTTCGA GGACGTGTCC 
TATGAGAGCA CACTGAAGGC ACTGGCTGAC GTGGAGGTCA CCTACACAGT GCAGAGGAAC 
ATTCTCGACT TCCCCCAGCA CGTGTCTCCG AACAAGGACA TCCGCGCAGC CAGCACAGAG 
GCTGACAAGA AGCTCTCAGA GTTTGATGTG GAGATGAGCA TGAGGCAGGA CGTGTACCAG 
AGGGTCGTGT GGCTGCAGGA GAAAATCCCG AAAGACTCAC TGAAGCCTGA GGCAGCTCGC 
TACCTGGAGC GGCTCATCAA GCTGGGCCGG AGAAACGGGC TCCACTTACC TCAGGACACA 
CAGGAGAAGA TCAAGAACAT CAAGAAGAGG CTGAGCCTGC TGTGCATCGA CTTCAACAAG 
AACCTGAATG AGGACACCAC CTTCCTGCCC TTCACGAGAG AGGAGCTGGG CGGGCTCCCC 
GAGGACTTCC TGAACTCCCT GGAGAAGACA GAGGACGGCA AACTGAAGGT CACCCTCAAG 
TATCCACACT ATTTCCCACT GCTGAAGAAG TGCCACGTGC CCGAGACACG ACGCCTGTTG 
GAGGAGGCCT TCAACTGTCG CTGCAAGGAG GAGAACTGTG CCATCCTGAA GGAGCTAGTG 
TCCCTGCGGG CGCAGAAGTC CAACCTGCTG GGATTCCGCA CACACGCAGA CTACGTCCTG 
GAGATGAACA TGGCCAAGAC CAGTCAGACA GTAGCCACCT TCCTAGATGA ACTGGCCCGG 
AAGCTGAAGC CGCTGGGTGA GCAGGAGCGT GCGGTGATCC TGGAGCTGAA GGAGGCAGAG 
TGCGCCAAGC GCGGGCTCCC ATTCGATGGG CGCATCCACG CATGGGACAT GCGGTACTAC 
ATGAACCAGG TGGAGGAGAC ACGCTACCGC GTAGACCAGA ACCTGCTGAA GGAGTACTTC 
CCCATGCAGG TGGTCACGCG TGGGCTGCTG GCCATCTACC AAGAGCTGCT GGGGCTGACC 
TTCACGCTGG AGGAAGGTGC CGCCGCCTGG CACGAAGACG TGCGGCTGTA CTCTGTGCGT 
GACGCCGCCT CTGGAGAGGA GATTGGCAAG TTCTACCTCG ACCTGTACCC CAGGGAAGGG 
AAGTATGGCC ACGCGGCCTG CTTCGGCCTG CAGCCTGGCT GCCTACGGCA GGATGGCAGC 
CGACAGCTGG CCATCGCAGC CATGGTGGCC AATTTCACCA AGCCCACACC TGACGTGCCT 
TCCCTGCTGC AGCACGATGA GGTGGAGACC TACTTCCACG AGTTCGGGCA CGTCATGCAC 
CAGCTCTGCT CACAGGCAGA GTTTGCTATG TTCAGTGGGA CCCACGTGGA GCGGGACTTT 
GTGGAGGCAC CGTCACAGAT GCTGGAGAAC TGGGTGTGGG AAAAGGAGCC ACTGATGCGC 
ATGTCCCAGC ATTACCGCAC AGGAGGCGAG GCCCCTGAGG ACCTCCTGGA GAAGCTCATC 
AAGTCTCGCC AGGCCAATGC AGGTCTCTTC AACCTTCGGC AGATTGTCCT TGCCAAGGTA 
GATCAGGTCC TGCACACACA GACAGATGTA GACCCAGCTG AGGAATATGC CCGGCTCTGC 
CAGGAGATCC TTGGGGTGCC AGCCACACCA GGTACCAACA TGCCAGCCAC TTTTGGCCAC 
CTCGCTGGTG GCTACGACGC TCAGTACTAT GGCTACTTGT GGAGTGAGGT GTACTCCATG 
GACATGTTCC ACACACGCTT CAAGCAGGAG GGTGTGCTTA GCCCCAAGGT TGGCATGGAT 
TACCGGACCA GCATCCTGAG GCCGGGGGGC TCTGAGGATG CCAGCACCAT GCTGAAGCAG 
TTCCTGGGCC GTGACCCCAA GCAAGACGCC TTCCTCCTGA GCAAGGGTCT GCAGGTAGAG 
GGCTGCGAGC CACCTGCGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742072.2
CDS	135..2198
Translation	MKPPAACAGDVVDTVSPCSTVNHLRWDLSAQQIRALTTQLIEQTKCVYDRVGAQDFEDVSYESTLKALADVEVTYTVQRNILDFPQHVSPNKDIRAASTEADKKLSEFDVEMSMRQDVYQRVVWLQEKIPKDSLKPEAARYLERLIKLGRRNGLHLPQDTQEKIKNIKKRLSLLCIDFNKNLNEDTTFLPFTREELGGLPEDFLNSLEKTEDGKLKVTLKYPHYFPLLKKCHVPETRRLLEEAFNCRCKEENCAILKELVSLRAQKSNLLGFRTHADYVLEMNMAKTSQTVATFLDELARKLKPLGEQERAVILELKEAECAKRGLPFDGRIHAWDMRYYMNQVEETRYRVDQNLLKEYFPMQVVTRGLLAIYQELLGLTFTLEEGAAAWHEDVRLYSVRDAASGEEIGKFYLDLYPREGKYGHAACFGLQPGCLRQDGSRQLAIAAMVANFTKPTPDVPSLLQHDEVETYFHEFGHVMHQLCSQAEFAMFSGTHVERDFVEAPSQMLENWVWEKEPLMRMSQHYRTGGEAPEDLLEKLIKSRQANAGLFNLRQIVLAKVDQVLHTQTDVDPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFHTRFKQEGVLSPKVGMDYRTSILRPGGSEDASTMLKQFLGRDPKQDAFLLSKGLQVEGCEPPAC

Target ORF information:

RefSeq Version	NM_172075.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thimet oligopeptidase 1 (Thop1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172075.2

ORF Insert Sequence:

ATGAAGCCCC CCGCAGCCTG TGCTGGGGAC GTGGTGGATA CAGTGTCACC ATGCTCCACC 
GTGAATCATC TGCGCTGGGA CCTGAGCGCA CAGCAGATCA GAGCTCTAAC CACACAGCTC 
ATCGAGCAGA CCAAGTGTGT GTACGACCGT GTGGGCGCCC AGGACTTCGA GGACGTGTCC 
TATGAGAGCA CACTGAAGGC ACTGGCTGAC GTGGAGGTCA CCTACACAGT GCAGAGGAAC 
ATTCTCGACT TCCCCCAGCA CGTGTCTCCG AACAAGGACA TCCGCGCAGC CAGCACAGAG 
GCTGACAAGA AGCTCTCAGA GTTTGATGTG GAGATGAGCA TGAGGCAGGA CGTGTACCAG 
AGGGTCGTGT GGCTGCAGGA GAAAATCCCG AAAGACTCAC TGAAGCCTGA GGCAGCTCGC 
TACCTGGAGC GGCTCATCAA GCTGGGCCGG AGAAACGGGC TCCACTTACC TCAGGACACA 
CAGGAGAAGA TCAAGAACAT CAAGAAGAGG CTGAGCCTGC TGTGCATCGA CTTCAACAAG 
AACCTGAATG AGGACACCAC CTTCCTGCCC TTCACGAGAG AGGAGCTGGG CGGGCTCCCC 
GAGGACTTCC TGAACTCCCT GGAGAAGACA GAGGACGGCA AACTGAAGGT CACCCTCAAG 
TATCCACACT ATTTCCCACT GCTGAAGAAG TGCCACGTGC CCGAGACACG ACGCCTGTTG 
GAGGAGGCCT TCAACTGTCG CTGCAAGGAG GAGAACTGTG CCATCCTGAA GGAGCTAGTG 
TCCCTGCGGG CGCAGAAGTC CAACCTGCTG GGATTCCGCA CACACGCAGA CTACGTCCTG 
GAGATGAACA TGGCCAAGAC CAGTCAGACA GTAGCCACCT TCCTAGATGA ACTGGCCCGG 
AAGCTGAAGC CGCTGGGTGA GCAGGAGCGT GCGGTGATCC TGGAGCTGAA GGAGGCAGAG 
TGCGCCAAGC GCGGGCTCCC ATTCGATGGG CGCATCCACG CATGGGACAT GCGGTACTAC 
ATGAACCAGG TGGAGGAGAC ACGCTACCGC GTAGACCAGA ACCTGCTGAA GGAGTACTTC 
CCCATGCAGG TGGTCACGCG TGGGCTGCTG GCCATCTACC AAGAGCTGCT GGGGCTGACC 
TTCACGCTGG AGGAAGGTGC CGCCGCCTGG CACGAAGACG TGCGGCTGTA CTCTGTGCGT 
GACGCCGCCT CTGGAGAGGA GATTGGCAAG TTCTACCTCG ACCTGTACCC CAGGGAAGGG 
AAGTATGGCC ACGCGGCCTG CTTCGGCCTG CAGCCTGGCT GCCTACGGCA GGATGGCAGC 
CGACAGCTGG CCATCGCAGC CATGGTGGCC AATTTCACCA AGCCCACACC TGACGTGCCT 
TCCCTGCTGC AGCACGATGA GGTGGAGACC TACTTCCACG AGTTCGGGCA CGTCATGCAC 
CAGCTCTGCT CACAGGCAGA GTTTGCTATG TTCAGTGGGA CCCACGTGGA GCGGGACTTT 
GTGGAGGCAC CGTCACAGAT GCTGGAGAAC TGGGTGTGGG AAAAGGAGCC ACTGATGCGC 
ATGTCCCAGC ATTACCGCAC AGGAGGCGAG GCCCCTGAGG ACCTCCTGGA GAAGCTCATC 
AAGTCTCGCC AGGCCAATGC AGGTCTCTTC AACCTTCGGC AGATTGTCCT TGCCAAGGTA 
GATCAGGTCC TGCACACACA GACAGATGTA GACCCAGCTG AGGAATATGC CCGGCTCTGC 
CAGGAGATCC TTGGGGTGCC AGCCACACCA GGTACCAACA TGCCAGCCAC TTTTGGCCAC 
CTCGCTGGTG GCTACGACGC TCAGTACTAT GGCTACTTGT GGAGTGAGGT GTACTCCATG 
GACATGTTCC ACACACGCTT CAAGCAGGAG GGTGTGCTTA GCCCCAAGGT TGGCATGGAT 
TACCGGACCA GCATCCTGAG GCCGGGGGGC TCTGAGGATG CCAGCACCAT GCTGAAGCAG 
TTCCTGGGCC GTGACCCCAA GCAAGACGCC TTCCTCCTGA GCAAGGGTCT GCAGGTAGAG 
GGCTGCGAGC CACCTGCGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thop1 ( XM_006240995.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241057.1 Rattus norvegicus thimet oligopeptidase 1 (Thop1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29906
Clone ID Related Accession (Same CDS sequence)	XM_006240995.3
Accession Version	XM_006240995.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	thimet oligopeptidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240995.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATGA GGCAGGACGT GTACCAGAGG GTCGTGTGGC TGCAGGAGAA AATCCCGAAA 
GACTCACTGA AGCCTGAGGC AGCTCGCTAC CTGGAGCGGC TCATCAAGCT GGGCCGGAGA 
AACGGGCTCC ACTTACCTCA GGACACACAG GAGAAGATCA AGAACATCAA GAAGAGGCTG 
AGCCTGCTGT GCATCGACTT CAACAAGAAC CTGAATGAGG ACACCACCTT CCTGCCCTTC 
ACGAGAGAGG AGCTGGGCGG GCTCCCCGAG GACTTCCTGA ACTCCCTGGA GAAGACAGAG 
GACGGCAAAC TGAAGGTCAC CCTCAAGTAT CCACACTATT TCCCACTGCT GAAGAAGTGC 
CACGTGCCCG AGACACGACG CCTGTTGGAG GAGGCCTTCA ACTGTCGCTG CAAGGAGGAG 
AACTGTGCCA TCCTGAAGGA GCTAGTGTCC CTGCGGGCGC AGAAGTCCAA CCTGCTGGGA 
TTCCGCACAC ACGCAGACTA CGTCCTGGAG ATGAACATGG CCAAGACCAG TCAGACAGTA 
GCCACCTTCC TAGATGAACT GGCCCGGAAG CTGAAGCCGC TGGGTGAGCA GGAGCGTGCG 
GTGATCCTGG AGCTGAAGGA GGCAGAGTGC GCCAAGCGCG GGCTCCCATT CGATGGGCGC 
ATCCACGCAT GGGACATGCG GTACTACATG AACCAGGTGG AGGAGACACG CTACCGCGTA 
GACCAGAACC TGCTGAAGGA GTACTTCCCC ATGCAGGTGG TCACGCGTGG GCTGCTGGCC 
ATCTACCAAG AGCTGCTGGG GCTGACCTTC ACGCTGGAGG AAGGTGCCGC CGCCTGGCAC 
GAAGACGTGC GGCTGTACTC TGTGCGTGAC GCCGCCTCTG GAGAGGAGAT TGGCAAGTTC 
TACCTCGACC TGTACCCCAG GGAAGGGAAG TATGGCCACG CGGCCTGCTT CGGCCTGCAG 
CCTGGCTGCC TACGGCAGGA TGGCAGCCGA CAGCTGGCCA TCGCAGCCAT GGTGGCCAAT 
TTCACCAAGC CCACACCTGA CGTGCCTTCC CTGCTGCAGC ACGATGAGGT GGAGACCTAC 
TTCCACGAGT TCGGGCACGT CATGCACCAG CTCTGCTCAC AGGCAGAGTT TGCTATGTTC 
AGTGGGACCC ACGTGGAGCG GGACTTTGTG GAGGCACCGT CACAGATGCT GGAGAACTGG 
GTGTGGGAAA AGGAGCCACT GATGCGCATG TCCCAGCATT ACCGCACAGG AGGCGAGGCC 
CCTGAGGACC TCCTGGAGAA GCTCATCAAG TCTCGCCAGG CCAATGCAGG TCTCTTCAAC 
CTTCGGCAGA TTGTCCTTGC CAAGGTAGAT CAGGTCCTGC ACACACAGAC AGATGTAGAC 
CCAGCTGAGG AATATGCCCG GCTCTGCCAG GAGATCCTTG GGGTGCCAGC CACACCAGGT 
ACCAACATGC CAGCCACTTT TGGCCACCTC GCTGGTGGCT ACGACGCTCA GTACTATGGC 
TACTTGTGGA GTGAGGTGTA CTCCATGGAC ATGTTCCACA CACGCTTCAA GCAGGAGGGT 
GTGCTTAGCC CCAAGGTTGG CATGGATTAC CGGACCAGCA TCCTGAGGCC GGGGGGCTCT 
GAGGATGCCA GCACCATGCT GAAGCAGTTC CTGGGCCGTG ACCCCAAGCA AGACGCCTTC 
CTCCTGAGCA AGGGTCTGCA GGTAGAGGGC TGCGAGCCAC CTGCGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241057.1
CDS	691..2421
Translation	MSMRQDVYQRVVWLQEKIPKDSLKPEAARYLERLIKLGRRNGLHLPQDTQEKIKNIKKRLSLLCIDFNKNLNEDTTFLPFTREELGGLPEDFLNSLEKTEDGKLKVTLKYPHYFPLLKKCHVPETRRLLEEAFNCRCKEENCAILKELVSLRAQKSNLLGFRTHADYVLEMNMAKTSQTVATFLDELARKLKPLGEQERAVILELKEAECAKRGLPFDGRIHAWDMRYYMNQVEETRYRVDQNLLKEYFPMQVVTRGLLAIYQELLGLTFTLEEGAAAWHEDVRLYSVRDAASGEEIGKFYLDLYPREGKYGHAACFGLQPGCLRQDGSRQLAIAAMVANFTKPTPDVPSLLQHDEVETYFHEFGHVMHQLCSQAEFAMFSGTHVERDFVEAPSQMLENWVWEKEPLMRMSQHYRTGGEAPEDLLEKLIKSRQANAGLFNLRQIVLAKVDQVLHTQTDVDPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFHTRFKQEGVLSPKVGMDYRTSILRPGGSEDASTMLKQFLGRDPKQDAFLLSKGLQVEGCEPPAC

Target ORF information:

RefSeq Version	XM_006240995.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thimet oligopeptidase 1 (Thop1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240995.3

ORF Insert Sequence:

ATGAGCATGA GGCAGGACGT GTACCAGAGG GTCGTGTGGC TGCAGGAGAA AATCCCGAAA 
GACTCACTGA AGCCTGAGGC AGCTCGCTAC CTGGAGCGGC TCATCAAGCT GGGCCGGAGA 
AACGGGCTCC ACTTACCTCA GGACACACAG GAGAAGATCA AGAACATCAA GAAGAGGCTG 
AGCCTGCTGT GCATCGACTT CAACAAGAAC CTGAATGAGG ACACCACCTT CCTGCCCTTC 
ACGAGAGAGG AGCTGGGCGG GCTCCCCGAG GACTTCCTGA ACTCCCTGGA GAAGACAGAG 
GACGGCAAAC TGAAGGTCAC CCTCAAGTAT CCACACTATT TCCCACTGCT GAAGAAGTGC 
CACGTGCCCG AGACACGACG CCTGTTGGAG GAGGCCTTCA ACTGTCGCTG CAAGGAGGAG 
AACTGTGCCA TCCTGAAGGA GCTAGTGTCC CTGCGGGCGC AGAAGTCCAA CCTGCTGGGA 
TTCCGCACAC ACGCAGACTA CGTCCTGGAG ATGAACATGG CCAAGACCAG TCAGACAGTA 
GCCACCTTCC TAGATGAACT GGCCCGGAAG CTGAAGCCGC TGGGTGAGCA GGAGCGTGCG 
GTGATCCTGG AGCTGAAGGA GGCAGAGTGC GCCAAGCGCG GGCTCCCATT CGATGGGCGC 
ATCCACGCAT GGGACATGCG GTACTACATG AACCAGGTGG AGGAGACACG CTACCGCGTA 
GACCAGAACC TGCTGAAGGA GTACTTCCCC ATGCAGGTGG TCACGCGTGG GCTGCTGGCC 
ATCTACCAAG AGCTGCTGGG GCTGACCTTC ACGCTGGAGG AAGGTGCCGC CGCCTGGCAC 
GAAGACGTGC GGCTGTACTC TGTGCGTGAC GCCGCCTCTG GAGAGGAGAT TGGCAAGTTC 
TACCTCGACC TGTACCCCAG GGAAGGGAAG TATGGCCACG CGGCCTGCTT CGGCCTGCAG 
CCTGGCTGCC TACGGCAGGA TGGCAGCCGA CAGCTGGCCA TCGCAGCCAT GGTGGCCAAT 
TTCACCAAGC CCACACCTGA CGTGCCTTCC CTGCTGCAGC ACGATGAGGT GGAGACCTAC 
TTCCACGAGT TCGGGCACGT CATGCACCAG CTCTGCTCAC AGGCAGAGTT TGCTATGTTC 
AGTGGGACCC ACGTGGAGCG GGACTTTGTG GAGGCACCGT CACAGATGCT GGAGAACTGG 
GTGTGGGAAA AGGAGCCACT GATGCGCATG TCCCAGCATT ACCGCACAGG AGGCGAGGCC 
CCTGAGGACC TCCTGGAGAA GCTCATCAAG TCTCGCCAGG CCAATGCAGG TCTCTTCAAC 
CTTCGGCAGA TTGTCCTTGC CAAGGTAGAT CAGGTCCTGC ACACACAGAC AGATGTAGAC 
CCAGCTGAGG AATATGCCCG GCTCTGCCAG GAGATCCTTG GGGTGCCAGC CACACCAGGT 
ACCAACATGC CAGCCACTTT TGGCCACCTC GCTGGTGGCT ACGACGCTCA GTACTATGGC 
TACTTGTGGA GTGAGGTGTA CTCCATGGAC ATGTTCCACA CACGCTTCAA GCAGGAGGGT 
GTGCTTAGCC CCAAGGTTGG CATGGATTAC CGGACCAGCA TCCTGAGGCC GGGGGGCTCT 
GAGGATGCCA GCACCATGCT GAAGCAGTTC CTGGGCCGTG ACCCCAAGCA AGACGCCTTC 
CTCCTGAGCA AGGGTCTGCA GGTAGAGGGC TGCGAGCCAC CTGCGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EP24.15 as a Potential Regulator of Kisspeptin Within the Neuroendocrine Hypothalamus.
Endocrinology157(2)820-30(2016 Feb)
Woitowich NC,Philibert KD,Leitermann RJ,Wungjiranirun M,Urban JH,Glucksman MJ

Angiotensin II-independent angiotensin-(1-7) formation in rat hippocampus: involvement of thimet oligopeptidase.
Hypertension (Dallas, Tex. : 1979)62(5)879-85(2013 Nov)
Pereira MG,Souza LL,Becari C,Duarte DA,Camacho FR,Oliveira JA,Gomes MD,Oliveira EB,Salgado MC,Garcia-Cairasco N,Costa-Neto CM

Natural intracellular peptides can modulate the interactions of mouse brain proteins and thimet oligopeptidase with 14-3-3? and calmodulin.
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Acute cocaine treatment increases thimet oligopeptidase in the striatum of rat brain.
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Increased expression of the oligopeptidase THOP1 is a neuroprotective response to Abeta toxicity.
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Thop1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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