Vps45 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps45
Entrez Gene ID	64516
Full Name	vacuolar protein sorting 45 homolog
Synonyms	Vsp45a
General protein information	Preferred Names vacuolar protein sorting 45 homolog Names vacuolar protein sorting-associated protein 45 rvps45 vesicular transport protein rvps45
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	expressed in brain and testis; may play a role in intracellular vesicle mediated transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_172072.2	NP_742069.1	vacuolar protein sorting-associated protein 45

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS45	NP_009190.2
Canis lupus familiaris (dog)	VPS45	XP_533042.2
Bos taurus (cattle)	VPS45	NP_001076970.1
Mus musculus (house mouse)	Vps45	NP_038869.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003980	XP_318430.3
Danio rerio (zebrafish)	vps45	NP_001243585.1
Schizosaccharomyces pombe (fission yeast)	vps45	NP_593083.1
Neurospora crassa	NCU06192	XP_963004.1
Rattus norvegicus (Norway rat)	Vps45	NP_742069.1
Saccharomyces cerevisiae (baker's yeast)	VPS45	NP_011420.3
Eremothecium gossypii	AGOS_AFL053W	NP_985495.2
Magnaporthe oryzae (rice blast fungus)	MGG_14755	XP_003713874.1
Arabidopsis thaliana (thale cress)	VPS45	NP_565150.1
Pan troglodytes (chimpanzee)	VPS45	XP_001167625.1
	VPS45	XP_001098282.1
Drosophila melanogaster (fruit fly)	Vps45	NP_649909.1
Caenorhabditis elegans (roundworm)	vps-45	NP_741714.1
Kluyveromyces lactis	KLLA0E18833g	XP_454802.1
Oryza sativa (rice)	Os02g0437800	NP_001046739.1
Xenopus tropicalis (tropical clawed frog)	vps45	NP_001072504.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.
Boyken J, Gr?nborg M, Riedel D, Urlaub H, Jahn R, Chua JJ
Neuron78(2)285-97(2013 Apr)

Munc18a controls SNARE assembly through its interaction with the syntaxin N-peptide.
Burkhardt P, Hattendorf DA, Weis WI, Fasshauer D
The EMBO journal27(7)923-33(2008 Apr)

Mammalian late vacuole protein sorting orthologues participate in early endosomal fusion and interact with the cytoskeleton.
Richardson SC, Winistorfer SC, Poupon V, Luzio JP, Piper RC
Molecular biology of the cell15(3)1197-210(2004 Mar)

Syntaxin 6 functions in trans-Golgi network vesicle trafficking.
Bock JB, Klumperman J, Davanger S, Scheller RH
Molecular biology of the cell8(7)1261-71(1997 Jul)

Molecular cloning of a mammalian homologue of the yeast vesicular transport protein vps45.
El-Husseini AE, Guthrie H, Snutch TP, Vincent SR
Biochimica et biophysica acta1325(1)8-12(1997 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vps45 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps45 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06045	NM_172072.2 Latest version!	Rattus norvegicus vacuolar protein sorting 45 homolog (Vps45), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$335.30 $479.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps45 ( NM_172072.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742069.1 Rattus norvegicus vacuolar protein sorting 45 homolog (Vps45), mRNA.

Price & Availability↑

CloneID	ORa06045
Clone ID Related Accession (Same CDS sequence)	NM_172072.2
Accession Version	NM_172072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	vacuolar protein sorting-associated protein 45
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081705.1. On Sep 16, 2004 this sequence version replaced NM_172072.1. ##Evidence-Data-START## Transcript exon combination :: BC081705.1, U81160.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906353 [ECO:0000348] ##Evidence-Data-END##

ATGAACGTGG TCTTTGCTGT GAAGCAGTAT ATTTCCAAAA TGATAGAAGA CAGCGGACCC 
GGCATGAAGG TACTTCTCAT GGATAAAGAG ACGACTGGTA TAGTGAGCAT GGTCTACACA 
CAGTCAGAGA TTCTTCAGAA GGAAGTATAC CTCTTCGAAC GAATTGATTC TCAAAATCGA 
GAGATCATGA AACACCTAAA AGCAATTTGT TTCCTTCGAC CTACAAAGGA GAATGTGGAT 
TCTCTGATCC AGGAGCTCCG AAGACCCAAG TATAGCATAT ATTTTATTTA TTTCAGTAAT 
GTGATCAGCA AGAGTGACGT GAAGTCCTTG GCTGAAGCTG ACGAGCAGGA AGTTGTGGCT 
GAAGTTCAGG AATTTTATGG AGATTATATT GCTGTGAATC CACATTTGTT TTCCCTCAAT 
ATCTTGGGCT GCTGTCAGGG TCGAAATTGG GATCCAGCCC AGCTATCCAG AACCACTCAA 
GGGCTGACCG CTCTCCTTTT GTCTCTGAAG AAGTGTCCCA TGATTCGTTA TCAGCTTTCA 
TCAGAGGCTG CAAAGAGACT GGGAGAGTGT GTTAAGCAAG TGATAAGTAA AGAGTATGAA 
CTCTTTGAGT TCCGGCGGAC AGAGGTTCCT CCACTGCTTC TCATTCTGGA TCGCTGCGAT 
GACGCCATCA CCCCACTGCT CAACCAGTGG ACATATCAGG CCATGGTCCA TGAACTACTG 
GGCATAAACA ACAACCGGAT TGATCTTTCC AGAGTGCCAG GAATCAGCAA AGACTTAAGA 
GAGGTGGTCC TGTCCGCTGA AAATGATGAA TTCTATGCTA ATAACATGTA CCTGAACTTT 
GCCGAGATTG GTAGCAATAT AAAGAATCTC ATGGAAGATT TCCAGAAGAA GAGACCGAAA 
GAGCAGCAAA AGCTAGAGTC CATAGCGGAC ATGAAGGCCT TTGTTGAAAA TTATCCACAA 
TTCAAGAAGA TGTCTGGGAC TGTCTCAAAG CATGTGACAG TCGTTGGAGA ACTGTCTCGG 
TTGGTCAGTG AACGAAACCT GCTGGAGGTT TCAGAGGTTG AGCAAGAACT GGCCTGTCAG 
AATGACCATT CTAGTGCTCT TCAGAATGTG AAGAGACTCC TGCAGAATCC GAAAGTTACA 
GAATTTGATG CAGTTCGCCT AGTGATGCTT TATGCTCTAC ATTATGAGCG CCACAGCAGC 
AACAGCCTGC CAGGGCTCAT AGTGGACCTC AGGAGTAAAG GTGTCGCTGA GAAATATCGG 
AAGCTTGTGT CTGCAGTTGT TGAATATGGT GGTAAACGGG TTAGAGGAAG TGACCTCTTC 
AGCCCCAAAG ATGCTGTGGC TATTACCAAA CAGTTCCTCA AAGGCCTGAA GGGAGTGGAA 
AATGTGTACA CCCAGCACCA GCCTTTTCTG CATGAGACCC TGGACCATCT CATCAAAGGG 
AAGCTTAAGG AAAACCTCTA TCCGTATTTA GGCCCCAGCA CACTCAGAGA CAGGCCTCAG 
GACATCATCG TGTTCGTTAT TGGAGGAGCC ACCTATGAAG AGGCACTGAC AGTCTATAAC 
CTCAACCGTA CCACTCCTGG AGTGAGGATC GTTCTGGGAG GAACAACAAT ACACAACACA 
AAAAGTTTCC TAGAGGAAGT CCTGGCTTCT GGGCTGCACA GCCGCAGCAG AGAGAGCTCA 
CAGGCCACCT CAAGGTCAGC AAGCAGAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742069.1
CDS	52..1764
Translation	MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDSLIQELRRPKYSIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLGECVKQVISKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKRPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQNDHSSALQNVKRLLQNPKVTEFDAVRLVMLYALHYERHSSNSLPGLIVDLRSKGVAEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGKLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTIHNTKSFLEEVLASGLHSRSRESSQATSRSASRR

Target ORF information:

RefSeq Version	NM_172072.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus vacuolar protein sorting 45 homolog (Vps45), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172072.2

ORF Insert Sequence:

ATGAACGTGG TCTTTGCTGT GAAGCAGTAT ATTTCCAAAA TGATAGAAGA CAGCGGACCC 
GGCATGAAGG TACTTCTCAT GGATAAAGAG ACGACTGGTA TAGTGAGCAT GGTCTACACA 
CAGTCAGAGA TTCTTCAGAA GGAAGTATAC CTCTTCGAAC GAATTGATTC TCAAAATCGA 
GAGATCATGA AACACCTAAA AGCAATTTGT TTCCTTCGAC CTACAAAGGA GAATGTGGAT 
TCTCTGATCC AGGAGCTCCG AAGACCCAAG TATAGCATAT ATTTTATTTA TTTCAGTAAT 
GTGATCAGCA AGAGTGACGT GAAGTCCTTG GCTGAAGCTG ACGAGCAGGA AGTTGTGGCT 
GAAGTTCAGG AATTTTATGG AGATTATATT GCTGTGAATC CACATTTGTT TTCCCTCAAT 
ATCTTGGGCT GCTGTCAGGG TCGAAATTGG GATCCAGCCC AGCTATCCAG AACCACTCAA 
GGGCTGACCG CTCTCCTTTT GTCTCTGAAG AAGTGTCCCA TGATTCGTTA TCAGCTTTCA 
TCAGAGGCTG CAAAGAGACT GGGAGAGTGT GTTAAGCAAG TGATAAGTAA AGAGTATGAA 
CTCTTTGAGT TCCGGCGGAC AGAGGTTCCT CCACTGCTTC TCATTCTGGA TCGCTGCGAT 
GACGCCATCA CCCCACTGCT CAACCAGTGG ACATATCAGG CCATGGTCCA TGAACTACTG 
GGCATAAACA ACAACCGGAT TGATCTTTCC AGAGTGCCAG GAATCAGCAA AGACTTAAGA 
GAGGTGGTCC TGTCCGCTGA AAATGATGAA TTCTATGCTA ATAACATGTA CCTGAACTTT 
GCCGAGATTG GTAGCAATAT AAAGAATCTC ATGGAAGATT TCCAGAAGAA GAGACCGAAA 
GAGCAGCAAA AGCTAGAGTC CATAGCGGAC ATGAAGGCCT TTGTTGAAAA TTATCCACAA 
TTCAAGAAGA TGTCTGGGAC TGTCTCAAAG CATGTGACAG TCGTTGGAGA ACTGTCTCGG 
TTGGTCAGTG AACGAAACCT GCTGGAGGTT TCAGAGGTTG AGCAAGAACT GGCCTGTCAG 
AATGACCATT CTAGTGCTCT TCAGAATGTG AAGAGACTCC TGCAGAATCC GAAAGTTACA 
GAATTTGATG CAGTTCGCCT AGTGATGCTT TATGCTCTAC ATTATGAGCG CCACAGCAGC 
AACAGCCTGC CAGGGCTCAT AGTGGACCTC AGGAGTAAAG GTGTCGCTGA GAAATATCGG 
AAGCTTGTGT CTGCAGTTGT TGAATATGGT GGTAAACGGG TTAGAGGAAG TGACCTCTTC 
AGCCCCAAAG ATGCTGTGGC TATTACCAAA CAGTTCCTCA AAGGCCTGAA GGGAGTGGAA 
AATGTGTACA CCCAGCACCA GCCTTTTCTG CATGAGACCC TGGACCATCT CATCAAAGGG 
AAGCTTAAGG AAAACCTCTA TCCGTATTTA GGCCCCAGCA CACTCAGAGA CAGGCCTCAG 
GACATCATCG TGTTCGTTAT TGGAGGAGCC ACCTATGAAG AGGCACTGAC AGTCTATAAC 
CTCAACCGTA CCACTCCTGG AGTGAGGATC GTTCTGGGAG GAACAACAAT ACACAACACA 
AAAAGTTTCC TAGAGGAAGT CCTGGCTTCT GGGCTGCACA GCCGCAGCAG AGAGAGCTCA 
CAGGCCACCT CAAGGTCAGC AAGCAGAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.
Neuron78(2)285-97(2013 Apr)
Boyken J,Gr?nborg M,Riedel D,Urlaub H,Jahn R,Chua JJ

Munc18a controls SNARE assembly through its interaction with the syntaxin N-peptide.
The EMBO journal27(7)923-33(2008 Apr)
Burkhardt P,Hattendorf DA,Weis WI,Fasshauer D

Mammalian late vacuole protein sorting orthologues participate in early endosomal fusion and interact with the cytoskeleton.
Molecular biology of the cell15(3)1197-210(2004 Mar)
Richardson SC,Winistorfer SC,Poupon V,Luzio JP,Piper RC

Syntaxin 6 functions in trans-Golgi network vesicle trafficking.
Molecular biology of the cell8(7)1261-71(1997 Jul)
Bock JB,Klumperman J,Davanger S,Scheller RH

Molecular cloning of a mammalian homologue of the yeast vesicular transport protein vps45.
Biochimica et biophysica acta1325(1)8-12(1997 Apr)
El-Husseini AE,Guthrie H,Snutch TP,Vincent SR

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Vps45 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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