Mfn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mfn2
Entrez Gene ID	64476
Full Name	mitofusin 2
Synonyms	HSG
General protein information	Preferred Names mitofusin 2 Names mitofusin-2 hypertension-related protein mitochondrial transmembrane GTPase FZO1A transmembrane GTPase MFN2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_017593611.1	XP_017449100.1	mitofusin-2 isoform X1
XM_006239402.3	XP_006239464.1	mitofusin-2 isoform X1
NM_130894.4	NP_570964.4	mitofusin-2
XM_006239403.2	XP_006239465.1	mitofusin-2 isoform X1
XM_008764288.2	XP_008762510.1	mitofusin-2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MFN2	XP_003307856.1
Canis lupus familiaris (dog)	MFN2	XP_005618048.1
Bos taurus (cattle)	MFN2	NP_001177198.1
Mus musculus (house mouse)	Mfn2	NP_001272849.1
Rattus norvegicus (Norway rat)	Mfn2	NP_570964.4
Danio rerio (zebrafish)	mfn2	NP_001121726.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001802	XP_321258.5
Caenorhabditis elegans (roundworm)	fzo-1	NP_495161.1
Rattus norvegicus (Norway rat)	LOC100911485	XP_003750134.1
Xenopus tropicalis (tropical clawed frog)	mfn2	NP_001016514.1
	MFN2	XP_002802232.1
Gallus gallus (chicken)	MFN2	XP_004947560.1
Homo sapiens (human)	MFN2	NP_001121132.1
Drosophila melanogaster (fruit fly)	Marf	NP_996357.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitofusin-2 mediated mitochondrial Ca
Liang RP, Jia JJ, Li JH, He N, Zhou YF, Jiang L, Bai T, Xie HY, Zhou L, Sun YL
World journal of gastroenterology23(38)6995-7008(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

that overexpression of Mfn2 can effectively inhibit the proliferation of reactive astrogliosis
Title: Overexpression of Mitofusin2 decreased the reactive astrocytes proliferation in vitro induced by oxygen-glucose deprivation/reoxygenation.

Mfn2 3' UTR was the target gene of miR-214. The miR-214 mimic had no significant effect on the mRNA expression of Mfn2.
Title: MicroRNA-214 Affects Fibroblast Differentiation of Adipose-Derived Mesenchymal Stem Cells by Targeting Mitofusin-2 during Pelvic Floor Dysfunction in SD Rats with Birth Trauma.

Study provides evidence that Mfn2 is negatively associated with cardiac remodeling.
Title: Candesartan cilexetil attenuated cardiac remodeling by improving expression and function of mitofusin 2 in SHR.

Downregulation of mitofusin 2 activates an apoptosis imbalance in vascular smooth muscle from hypoxic pulmonary hypertension animals.
Title: Mitofusin 2 Downregulation Triggers Pulmonary Artery Smooth Muscle Cell Proliferation and Apoptosis Imbalance in Rats With Hypoxic Pulmonary Hypertension Via the PI3K/Akt and Mitochondrial Apoptosis Pathways.

Rg1 through modulation of GDH and MFN2 maintained mitochondrial dynamics that resulted in protection against H/R-induced cardiomyocyte injury. All these results put forward a new protective mechanism of Rg1 on the therapeutic potential in cardiac I/R disorders.
Title: Rg1 prevents myocardial hypoxia/reoxygenation injury by regulating mitochondrial dynamics imbalance via modulation of glutamate dehydrogenase and mitofusin 2.

The following Mfn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mfn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12247	NM_130894.4 Latest version!	Rattus norvegicus mitofusin 2 (Mfn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa12247	XM_017593611.1 Latest version!	Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa12247	XM_006239403.2 Latest version!	Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa12247	XM_008764288.2 Latest version!	Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa12247	XM_006239402.3 Latest version!	Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mfn2 ( NM_130894.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_570964.4 Rattus norvegicus mitofusin 2 (Mfn2), mRNA.

Price & Availability↑

CloneID	ORa12247
Clone ID Related Accession (Same CDS sequence)	XM_017593611.1 , XM_006239402.3 , NM_130894.4 , XM_006239403.2 , XM_008764288.2
Accession Version	NM_130894.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	mitofusin-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC070880.1. On Aug 31, 2012 this sequence version replaced NM_130894.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070880.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCCCTGC TCTTTTCTCG ATGCAACTCC ATCGTCACCG TCAAGAAGGA TAAGCGACAC 
ATGGCTGAGG TGAATGCTTC CCCTCTCAAG CACTTTGTCA CTGCCAAGAA AAAGATCAAT 
GGCATCTTTG AGCAGCTGGG GGCCTACATC CAAGAGAGTG CCGGCTTCCT TGAAGACACC 
CACAGGAATA CAGAACTGGA CCCAGTTACT ACAGAAGAGC AGGTCCTGGA TGTCAAAGGG 
TACCTGTCCA AGGTCAGGGG AATCAGCGAG GTGCTGGCCA GGCGGCACAT GAAGGTGGCT 
TTTTTTGGCC GGACGAGCAA TGGGAAGAGC ACCGTGATCA ATGCCATGCT TTGGGACAAA 
GTTCTGCCAT CTGGGATTGG CCACACCACC AATTGCTTCC TGCGGGTTGG GGGCACGGAT 
GGCCACGAGG CCTTCCTCCT CACGGAGGGC TCAGAAGAGA AGAAGAGTGT CAAGACCGTG 
AACCAGCTAG CCCATGCCCT CCATCAGGAT GAGCAGCTGC ATGCAGGCAG CCTGGTGAGT 
GTGATGTGGC CCAACTCCAA GTGTCCACTT CTGAAGGATG ACCTCGTGCT GATGGACAGC 
CCTGGGATCG ATGTCACCAC GGAGCTGGAC AGCTGGATTG ATAAGTTTTG CCTGGATGCT 
GATGTGTTTG TACTGGTGGC CAACTCAGAG TCCACGCTGA TGCAGACGGA GAAGCAGTTC 
TTCCACAAAG TGAGTGAGCG TCTCTCCCGG CCCAACATCT TCATCCTGAA CAACCGCTGG 
GATGCGTCTG CCTCGGAGCC TGAGTACATG GAGGAGGTGC GGCGGCAGCA CATGGAACGC 
TGCACCAGCT TTCTGGTGGA TGAGCTAGGC GTGGTGGATC GAGCTCAGGC CGGGGACCGG 
ATCTTCTTTG TGTCTGCCAA GGAGGTGCTC AGTGCCAGGG TCCAGAAAGC CCAGGGCATG 
CCAGAAGGAG GTGGCGCTCT TGCAGAAGGT TTTCAAGTGA GGATGTTTGA GTTTCAGAAT 
TTCGAGAGGC GATTTGAGGA GTGCATTTCC CAGTCTGCAG TAAAGACCAA ATTTGAGCAG 
CACACAGTCC GGGCCAAGCA GATTGCAGAG GCCGTCCGTC TCATCATGGA TTCCCTGCAC 
ATTGCGGCTC AGGAGCAGCG GGTTTATTGT CTTGAAATGC GGGAAGAGCG GCAAGACCGG 
CTGAGATTCA TTGACAAGCA GCTGGAGCTC CTGGCTCAAG ACTATAAGCT ACGGATTAAG 
CAGATGACAG AGGAAGTGGA AAGGCAGGTG TCCACAGCCA TGGCTGAAGA GATCAGGCGC 
CTCTCTGTGC TGGTTGACGA GTACCAGATG GACTTTCACC CATCCCCAGT TGTCCTCAAG 
GTTTATAAGA ATGAACTGCA CCGCCATATA GAGGAAGGCC TGGGACGAAA CATGTCTGAC 
CGCTGCTCCA CAGCCATTGC CAGTTCACTG CAGACTATGC AGCAAGACAT GATAGACGGC 
TTGAAGCCCC TTCTTCCTGT GTCTGTGCGG AATCAGATAG ACATGCTGGT CCCTCGACAG 
TGTTTCTCCC TCAGCTATGA CCTGAACTGT GACAAGCTGT GCGCTGACTT TCAGGAGGAC 
ATCGAATTCC ATTTCTCCCT TGGATGGACT ATGCTAGTGA ATAGGTTCCT GGGCCCTAAG 
AATAGCCGGC GGGCCTTGCT GGGCTACAAT GATCAGGTTC AGCGTCCTCT CCCTCTGACA 
CCTGCCAACC CCAGCATGCC ACCCTTGCCA CAGGGCTCCC TCACCCAGGA GGAGCTCATG 
GTCTCCATGG TTACTGGCCT GGCCTCTCTG ACTTCCAGGA CCTCTATGGG CATTCTCGTG 
GTCGGAGGAG TGGTGTGGAA GGCAGTGGGC TGGAGACTCA TCGCCCTCTC CTTTGGACTG 
TATGGCCTCC TGTACGTGTA TGAGCGGCTG ACCTGGACCA CCAGAGCCAA GGAGAGGGCC 
TTCAAGCGCC AGTTTGTGGA GTATGCCAGT GAGAAGCTGC AGCTCATTAT CAGTTATACC 
GGCTCTAACT GCAGCCACCA AGTTCAGCAA GAATTGTCTG GGACATTTGC TCATCTGTGC 
CAGCAAGTTG ACATCACCCG GGATAATCTG GAGCAGGAAA TTGCTGCCAT GAACAAGAAA 
GTTGAGGCTC TGGATTCACT TCAGAGCAAA GCCAAATTGC TCAGGAATAA AGCTGGCTGG 
TTGGACAGCG AACTCAACAT GTTCATACAC CAGTACCTGC AGCCCAGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570964.4
CDS	148..2421
Translation	MSLLFSRCNSIVTVKKDKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESAGFLEDTHRNTELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVGGTDGHEAFLLTEGSEEKKSVKTVNQLAHALHQDEQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKQFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRAQAGDRIFFVSAKEVLSARVQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHIAAQEQRVYCLEMREERQDRLRFIDKQLELLAQDYKLRIKQMTEEVERQVSTAMAEEIRRLSVLVDEYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAIASSLQTMQQDMIDGLKPLLPVSVRNQIDMLVPRQCFSLSYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALLGYNDQVQRPLPLTPANPSMPPLPQGSLTQEELMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTRAKERAFKRQFVEYASEKLQLIISYTGSNCSHQVQQELSGTFAHLCQQVDITRDNLEQEIAAMNKKVEALDSLQSKAKLLRNKAGWLDSELNMFIHQYLQPSR

Target ORF information:

RefSeq Version	NM_130894.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitofusin 2 (Mfn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130894.4

ORF Insert Sequence:

ATGTCCCTGC TCTTTTCTCG ATGCAACTCC ATCGTCACCG TCAAGAAGGA TAAGCGACAC 
ATGGCTGAGG TGAATGCTTC CCCTCTCAAG CACTTTGTCA CTGCCAAGAA AAAGATCAAT 
GGCATCTTTG AGCAGCTGGG GGCCTACATC CAAGAGAGTG CCGGCTTCCT TGAAGACACC 
CACAGGAATA CAGAACTGGA CCCAGTTACT ACAGAAGAGC AGGTCCTGGA TGTCAAAGGG 
TACCTGTCCA AGGTCAGGGG AATCAGCGAG GTGCTGGCCA GGCGGCACAT GAAGGTGGCT 
TTTTTTGGCC GGACGAGCAA TGGGAAGAGC ACCGTGATCA ATGCCATGCT TTGGGACAAA 
GTTCTGCCAT CTGGGATTGG CCACACCACC AATTGCTTCC TGCGGGTTGG GGGCACGGAT 
GGCCACGAGG CCTTCCTCCT CACGGAGGGC TCAGAAGAGA AGAAGAGTGT CAAGACCGTG 
AACCAGCTAG CCCATGCCCT CCATCAGGAT GAGCAGCTGC ATGCAGGCAG CCTGGTGAGT 
GTGATGTGGC CCAACTCCAA GTGTCCACTT CTGAAGGATG ACCTCGTGCT GATGGACAGC 
CCTGGGATCG ATGTCACCAC GGAGCTGGAC AGCTGGATTG ATAAGTTTTG CCTGGATGCT 
GATGTGTTTG TACTGGTGGC CAACTCAGAG TCCACGCTGA TGCAGACGGA GAAGCAGTTC 
TTCCACAAAG TGAGTGAGCG TCTCTCCCGG CCCAACATCT TCATCCTGAA CAACCGCTGG 
GATGCGTCTG CCTCGGAGCC TGAGTACATG GAGGAGGTGC GGCGGCAGCA CATGGAACGC 
TGCACCAGCT TTCTGGTGGA TGAGCTAGGC GTGGTGGATC GAGCTCAGGC CGGGGACCGG 
ATCTTCTTTG TGTCTGCCAA GGAGGTGCTC AGTGCCAGGG TCCAGAAAGC CCAGGGCATG 
CCAGAAGGAG GTGGCGCTCT TGCAGAAGGT TTTCAAGTGA GGATGTTTGA GTTTCAGAAT 
TTCGAGAGGC GATTTGAGGA GTGCATTTCC CAGTCTGCAG TAAAGACCAA ATTTGAGCAG 
CACACAGTCC GGGCCAAGCA GATTGCAGAG GCCGTCCGTC TCATCATGGA TTCCCTGCAC 
ATTGCGGCTC AGGAGCAGCG GGTTTATTGT CTTGAAATGC GGGAAGAGCG GCAAGACCGG 
CTGAGATTCA TTGACAAGCA GCTGGAGCTC CTGGCTCAAG ACTATAAGCT ACGGATTAAG 
CAGATGACAG AGGAAGTGGA AAGGCAGGTG TCCACAGCCA TGGCTGAAGA GATCAGGCGC 
CTCTCTGTGC TGGTTGACGA GTACCAGATG GACTTTCACC CATCCCCAGT TGTCCTCAAG 
GTTTATAAGA ATGAACTGCA CCGCCATATA GAGGAAGGCC TGGGACGAAA CATGTCTGAC 
CGCTGCTCCA CAGCCATTGC CAGTTCACTG CAGACTATGC AGCAAGACAT GATAGACGGC 
TTGAAGCCCC TTCTTCCTGT GTCTGTGCGG AATCAGATAG ACATGCTGGT CCCTCGACAG 
TGTTTCTCCC TCAGCTATGA CCTGAACTGT GACAAGCTGT GCGCTGACTT TCAGGAGGAC 
ATCGAATTCC ATTTCTCCCT TGGATGGACT ATGCTAGTGA ATAGGTTCCT GGGCCCTAAG 
AATAGCCGGC GGGCCTTGCT GGGCTACAAT GATCAGGTTC AGCGTCCTCT CCCTCTGACA 
CCTGCCAACC CCAGCATGCC ACCCTTGCCA CAGGGCTCCC TCACCCAGGA GGAGCTCATG 
GTCTCCATGG TTACTGGCCT GGCCTCTCTG ACTTCCAGGA CCTCTATGGG CATTCTCGTG 
GTCGGAGGAG TGGTGTGGAA GGCAGTGGGC TGGAGACTCA TCGCCCTCTC CTTTGGACTG 
TATGGCCTCC TGTACGTGTA TGAGCGGCTG ACCTGGACCA CCAGAGCCAA GGAGAGGGCC 
TTCAAGCGCC AGTTTGTGGA GTATGCCAGT GAGAAGCTGC AGCTCATTAT CAGTTATACC 
GGCTCTAACT GCAGCCACCA AGTTCAGCAA GAATTGTCTG GGACATTTGC TCATCTGTGC 
CAGCAAGTTG ACATCACCCG GGATAATCTG GAGCAGGAAA TTGCTGCCAT GAACAAGAAA 
GTTGAGGCTC TGGATTCACT TCAGAGCAAA GCCAAATTGC TCAGGAATAA AGCTGGCTGG 
TTGGACAGCG AACTCAACAT GTTCATACAC CAGTACCTGC AGCCCAGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfn2 ( XM_017593611.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449100.1 Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa12247
Clone ID Related Accession (Same CDS sequence)	XM_017593611.1 , XM_006239402.3 , NM_130894.4 , XM_006239403.2 , XM_008764288.2
Accession Version	XM_017593611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitofusin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGC TCTTTTCTCG ATGCAACTCC ATCGTCACCG TCAAGAAGGA TAAGCGACAC 
ATGGCTGAGG TGAATGCTTC CCCTCTCAAG CACTTTGTCA CTGCCAAGAA AAAGATCAAT 
GGCATCTTTG AGCAGCTGGG GGCCTACATC CAAGAGAGTG CCGGCTTCCT TGAAGACACC 
CACAGGAATA CAGAACTGGA CCCAGTTACT ACAGAAGAGC AGGTCCTGGA TGTCAAAGGG 
TACCTGTCCA AGGTCAGGGG AATCAGCGAG GTGCTGGCCA GGCGGCACAT GAAGGTGGCT 
TTTTTTGGCC GGACGAGCAA TGGGAAGAGC ACCGTGATCA ATGCCATGCT TTGGGACAAA 
GTTCTGCCAT CTGGGATTGG CCACACCACC AATTGCTTCC TGCGGGTTGG GGGCACGGAT 
GGCCACGAGG CCTTCCTCCT CACGGAGGGC TCAGAAGAGA AGAAGAGTGT CAAGACCGTG 
AACCAGCTAG CCCATGCCCT CCATCAGGAT GAGCAGCTGC ATGCAGGCAG CCTGGTGAGT 
GTGATGTGGC CCAACTCCAA GTGTCCACTT CTGAAGGATG ACCTCGTGCT GATGGACAGC 
CCTGGGATCG ATGTCACCAC GGAGCTGGAC AGCTGGATTG ATAAGTTTTG CCTGGATGCT 
GATGTGTTTG TACTGGTGGC CAACTCAGAG TCCACGCTGA TGCAGACGGA GAAGCAGTTC 
TTCCACAAAG TGAGTGAGCG TCTCTCCCGG CCCAACATCT TCATCCTGAA CAACCGCTGG 
GATGCGTCTG CCTCGGAGCC TGAGTACATG GAGGAGGTGC GGCGGCAGCA CATGGAACGC 
TGCACCAGCT TTCTGGTGGA TGAGCTAGGC GTGGTGGATC GAGCTCAGGC CGGGGACCGG 
ATCTTCTTTG TGTCTGCCAA GGAGGTGCTC AGTGCCAGGG TCCAGAAAGC CCAGGGCATG 
CCAGAAGGAG GTGGCGCTCT TGCAGAAGGT TTTCAAGTGA GGATGTTTGA GTTTCAGAAT 
TTCGAGAGGC GATTTGAGGA GTGCATTTCC CAGTCTGCAG TAAAGACCAA ATTTGAGCAG 
CACACAGTCC GGGCCAAGCA GATTGCAGAG GCCGTCCGTC TCATCATGGA TTCCCTGCAC 
ATTGCGGCTC AGGAGCAGCG GGTTTATTGT CTTGAAATGC GGGAAGAGCG GCAAGACCGG 
CTGAGATTCA TTGACAAGCA GCTGGAGCTC CTGGCTCAAG ACTATAAGCT ACGGATTAAG 
CAGATGACAG AGGAAGTGGA AAGGCAGGTG TCCACAGCCA TGGCTGAAGA GATCAGGCGC 
CTCTCTGTGC TGGTTGACGA GTACCAGATG GACTTTCACC CATCCCCAGT TGTCCTCAAG 
GTTTATAAGA ATGAACTGCA CCGCCATATA GAGGAAGGCC TGGGACGAAA CATGTCTGAC 
CGCTGCTCCA CAGCCATTGC CAGTTCACTG CAGACTATGC AGCAAGACAT GATAGACGGC 
TTGAAGCCCC TTCTTCCTGT GTCTGTGCGG AATCAGATAG ACATGCTGGT CCCTCGACAG 
TGTTTCTCCC TCAGCTATGA CCTGAACTGT GACAAGCTGT GCGCTGACTT TCAGGAGGAC 
ATCGAATTCC ATTTCTCCCT TGGATGGACT ATGCTAGTGA ATAGGTTCCT GGGCCCTAAG 
AATAGCCGGC GGGCCTTGCT GGGCTACAAT GATCAGGTTC AGCGTCCTCT CCCTCTGACA 
CCTGCCAACC CCAGCATGCC ACCCTTGCCA CAGGGCTCCC TCACCCAGGA GGAGCTCATG 
GTCTCCATGG TTACTGGCCT GGCCTCTCTG ACTTCCAGGA CCTCTATGGG CATTCTCGTG 
GTCGGAGGAG TGGTGTGGAA GGCAGTGGGC TGGAGACTCA TCGCCCTCTC CTTTGGACTG 
TATGGCCTCC TGTACGTGTA TGAGCGGCTG ACCTGGACCA CCAGAGCCAA GGAGAGGGCC 
TTCAAGCGCC AGTTTGTGGA GTATGCCAGT GAGAAGCTGC AGCTCATTAT CAGTTATACC 
GGCTCTAACT GCAGCCACCA AGTTCAGCAA GAATTGTCTG GGACATTTGC TCATCTGTGC 
CAGCAAGTTG ACATCACCCG GGATAATCTG GAGCAGGAAA TTGCTGCCAT GAACAAGAAA 
GTTGAGGCTC TGGATTCACT TCAGAGCAAA GCCAAATTGC TCAGGAATAA AGCTGGCTGG 
TTGGACAGCG AACTCAACAT GTTCATACAC CAGTACCTGC AGCCCAGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449100.1
CDS	394..2667
Translation	MSLLFSRCNSIVTVKKDKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESAGFLEDTHRNTELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVGGTDGHEAFLLTEGSEEKKSVKTVNQLAHALHQDEQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKQFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRAQAGDRIFFVSAKEVLSARVQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHIAAQEQRVYCLEMREERQDRLRFIDKQLELLAQDYKLRIKQMTEEVERQVSTAMAEEIRRLSVLVDEYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAIASSLQTMQQDMIDGLKPLLPVSVRNQIDMLVPRQCFSLSYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALLGYNDQVQRPLPLTPANPSMPPLPQGSLTQEELMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTRAKERAFKRQFVEYASEKLQLIISYTGSNCSHQVQQELSGTFAHLCQQVDITRDNLEQEIAAMNKKVEALDSLQSKAKLLRNKAGWLDSELNMFIHQYLQPSR

Target ORF information:

RefSeq Version	XM_017593611.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593611.1

ORF Insert Sequence:

ATGTCCCTGC TCTTTTCTCG ATGCAACTCC ATCGTCACCG TCAAGAAGGA TAAGCGACAC 
ATGGCTGAGG TGAATGCTTC CCCTCTCAAG CACTTTGTCA CTGCCAAGAA AAAGATCAAT 
GGCATCTTTG AGCAGCTGGG GGCCTACATC CAAGAGAGTG CCGGCTTCCT TGAAGACACC 
CACAGGAATA CAGAACTGGA CCCAGTTACT ACAGAAGAGC AGGTCCTGGA TGTCAAAGGG 
TACCTGTCCA AGGTCAGGGG AATCAGCGAG GTGCTGGCCA GGCGGCACAT GAAGGTGGCT 
TTTTTTGGCC GGACGAGCAA TGGGAAGAGC ACCGTGATCA ATGCCATGCT TTGGGACAAA 
GTTCTGCCAT CTGGGATTGG CCACACCACC AATTGCTTCC TGCGGGTTGG GGGCACGGAT 
GGCCACGAGG CCTTCCTCCT CACGGAGGGC TCAGAAGAGA AGAAGAGTGT CAAGACCGTG 
AACCAGCTAG CCCATGCCCT CCATCAGGAT GAGCAGCTGC ATGCAGGCAG CCTGGTGAGT 
GTGATGTGGC CCAACTCCAA GTGTCCACTT CTGAAGGATG ACCTCGTGCT GATGGACAGC 
CCTGGGATCG ATGTCACCAC GGAGCTGGAC AGCTGGATTG ATAAGTTTTG CCTGGATGCT 
GATGTGTTTG TACTGGTGGC CAACTCAGAG TCCACGCTGA TGCAGACGGA GAAGCAGTTC 
TTCCACAAAG TGAGTGAGCG TCTCTCCCGG CCCAACATCT TCATCCTGAA CAACCGCTGG 
GATGCGTCTG CCTCGGAGCC TGAGTACATG GAGGAGGTGC GGCGGCAGCA CATGGAACGC 
TGCACCAGCT TTCTGGTGGA TGAGCTAGGC GTGGTGGATC GAGCTCAGGC CGGGGACCGG 
ATCTTCTTTG TGTCTGCCAA GGAGGTGCTC AGTGCCAGGG TCCAGAAAGC CCAGGGCATG 
CCAGAAGGAG GTGGCGCTCT TGCAGAAGGT TTTCAAGTGA GGATGTTTGA GTTTCAGAAT 
TTCGAGAGGC GATTTGAGGA GTGCATTTCC CAGTCTGCAG TAAAGACCAA ATTTGAGCAG 
CACACAGTCC GGGCCAAGCA GATTGCAGAG GCCGTCCGTC TCATCATGGA TTCCCTGCAC 
ATTGCGGCTC AGGAGCAGCG GGTTTATTGT CTTGAAATGC GGGAAGAGCG GCAAGACCGG 
CTGAGATTCA TTGACAAGCA GCTGGAGCTC CTGGCTCAAG ACTATAAGCT ACGGATTAAG 
CAGATGACAG AGGAAGTGGA AAGGCAGGTG TCCACAGCCA TGGCTGAAGA GATCAGGCGC 
CTCTCTGTGC TGGTTGACGA GTACCAGATG GACTTTCACC CATCCCCAGT TGTCCTCAAG 
GTTTATAAGA ATGAACTGCA CCGCCATATA GAGGAAGGCC TGGGACGAAA CATGTCTGAC 
CGCTGCTCCA CAGCCATTGC CAGTTCACTG CAGACTATGC AGCAAGACAT GATAGACGGC 
TTGAAGCCCC TTCTTCCTGT GTCTGTGCGG AATCAGATAG ACATGCTGGT CCCTCGACAG 
TGTTTCTCCC TCAGCTATGA CCTGAACTGT GACAAGCTGT GCGCTGACTT TCAGGAGGAC 
ATCGAATTCC ATTTCTCCCT TGGATGGACT ATGCTAGTGA ATAGGTTCCT GGGCCCTAAG 
AATAGCCGGC GGGCCTTGCT GGGCTACAAT GATCAGGTTC AGCGTCCTCT CCCTCTGACA 
CCTGCCAACC CCAGCATGCC ACCCTTGCCA CAGGGCTCCC TCACCCAGGA GGAGCTCATG 
GTCTCCATGG TTACTGGCCT GGCCTCTCTG ACTTCCAGGA CCTCTATGGG CATTCTCGTG 
GTCGGAGGAG TGGTGTGGAA GGCAGTGGGC TGGAGACTCA TCGCCCTCTC CTTTGGACTG 
TATGGCCTCC TGTACGTGTA TGAGCGGCTG ACCTGGACCA CCAGAGCCAA GGAGAGGGCC 
TTCAAGCGCC AGTTTGTGGA GTATGCCAGT GAGAAGCTGC AGCTCATTAT CAGTTATACC 
GGCTCTAACT GCAGCCACCA AGTTCAGCAA GAATTGTCTG GGACATTTGC TCATCTGTGC 
CAGCAAGTTG ACATCACCCG GGATAATCTG GAGCAGGAAA TTGCTGCCAT GAACAAGAAA 
GTTGAGGCTC TGGATTCACT TCAGAGCAAA GCCAAATTGC TCAGGAATAA AGCTGGCTGG 
TTGGACAGCG AACTCAACAT GTTCATACAC CAGTACCTGC AGCCCAGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfn2 ( XM_006239402.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239464.1 Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X1, mRNA.

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CloneID	ORa12247
Clone ID Related Accession (Same CDS sequence)	XM_017593611.1 , XM_006239402.3 , NM_130894.4 , XM_006239403.2 , XM_008764288.2
Accession Version	XM_006239402.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitofusin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239402.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGC TCTTTTCTCG ATGCAACTCC ATCGTCACCG TCAAGAAGGA TAAGCGACAC 
ATGGCTGAGG TGAATGCTTC CCCTCTCAAG CACTTTGTCA CTGCCAAGAA AAAGATCAAT 
GGCATCTTTG AGCAGCTGGG GGCCTACATC CAAGAGAGTG CCGGCTTCCT TGAAGACACC 
CACAGGAATA CAGAACTGGA CCCAGTTACT ACAGAAGAGC AGGTCCTGGA TGTCAAAGGG 
TACCTGTCCA AGGTCAGGGG AATCAGCGAG GTGCTGGCCA GGCGGCACAT GAAGGTGGCT 
TTTTTTGGCC GGACGAGCAA TGGGAAGAGC ACCGTGATCA ATGCCATGCT TTGGGACAAA 
GTTCTGCCAT CTGGGATTGG CCACACCACC AATTGCTTCC TGCGGGTTGG GGGCACGGAT 
GGCCACGAGG CCTTCCTCCT CACGGAGGGC TCAGAAGAGA AGAAGAGTGT CAAGACCGTG 
AACCAGCTAG CCCATGCCCT CCATCAGGAT GAGCAGCTGC ATGCAGGCAG CCTGGTGAGT 
GTGATGTGGC CCAACTCCAA GTGTCCACTT CTGAAGGATG ACCTCGTGCT GATGGACAGC 
CCTGGGATCG ATGTCACCAC GGAGCTGGAC AGCTGGATTG ATAAGTTTTG CCTGGATGCT 
GATGTGTTTG TACTGGTGGC CAACTCAGAG TCCACGCTGA TGCAGACGGA GAAGCAGTTC 
TTCCACAAAG TGAGTGAGCG TCTCTCCCGG CCCAACATCT TCATCCTGAA CAACCGCTGG 
GATGCGTCTG CCTCGGAGCC TGAGTACATG GAGGAGGTGC GGCGGCAGCA CATGGAACGC 
TGCACCAGCT TTCTGGTGGA TGAGCTAGGC GTGGTGGATC GAGCTCAGGC CGGGGACCGG 
ATCTTCTTTG TGTCTGCCAA GGAGGTGCTC AGTGCCAGGG TCCAGAAAGC CCAGGGCATG 
CCAGAAGGAG GTGGCGCTCT TGCAGAAGGT TTTCAAGTGA GGATGTTTGA GTTTCAGAAT 
TTCGAGAGGC GATTTGAGGA GTGCATTTCC CAGTCTGCAG TAAAGACCAA ATTTGAGCAG 
CACACAGTCC GGGCCAAGCA GATTGCAGAG GCCGTCCGTC TCATCATGGA TTCCCTGCAC 
ATTGCGGCTC AGGAGCAGCG GGTTTATTGT CTTGAAATGC GGGAAGAGCG GCAAGACCGG 
CTGAGATTCA TTGACAAGCA GCTGGAGCTC CTGGCTCAAG ACTATAAGCT ACGGATTAAG 
CAGATGACAG AGGAAGTGGA AAGGCAGGTG TCCACAGCCA TGGCTGAAGA GATCAGGCGC 
CTCTCTGTGC TGGTTGACGA GTACCAGATG GACTTTCACC CATCCCCAGT TGTCCTCAAG 
GTTTATAAGA ATGAACTGCA CCGCCATATA GAGGAAGGCC TGGGACGAAA CATGTCTGAC 
CGCTGCTCCA CAGCCATTGC CAGTTCACTG CAGACTATGC AGCAAGACAT GATAGACGGC 
TTGAAGCCCC TTCTTCCTGT GTCTGTGCGG AATCAGATAG ACATGCTGGT CCCTCGACAG 
TGTTTCTCCC TCAGCTATGA CCTGAACTGT GACAAGCTGT GCGCTGACTT TCAGGAGGAC 
ATCGAATTCC ATTTCTCCCT TGGATGGACT ATGCTAGTGA ATAGGTTCCT GGGCCCTAAG 
AATAGCCGGC GGGCCTTGCT GGGCTACAAT GATCAGGTTC AGCGTCCTCT CCCTCTGACA 
CCTGCCAACC CCAGCATGCC ACCCTTGCCA CAGGGCTCCC TCACCCAGGA GGAGCTCATG 
GTCTCCATGG TTACTGGCCT GGCCTCTCTG ACTTCCAGGA CCTCTATGGG CATTCTCGTG 
GTCGGAGGAG TGGTGTGGAA GGCAGTGGGC TGGAGACTCA TCGCCCTCTC CTTTGGACTG 
TATGGCCTCC TGTACGTGTA TGAGCGGCTG ACCTGGACCA CCAGAGCCAA GGAGAGGGCC 
TTCAAGCGCC AGTTTGTGGA GTATGCCAGT GAGAAGCTGC AGCTCATTAT CAGTTATACC 
GGCTCTAACT GCAGCCACCA AGTTCAGCAA GAATTGTCTG GGACATTTGC TCATCTGTGC 
CAGCAAGTTG ACATCACCCG GGATAATCTG GAGCAGGAAA TTGCTGCCAT GAACAAGAAA 
GTTGAGGCTC TGGATTCACT TCAGAGCAAA GCCAAATTGC TCAGGAATAA AGCTGGCTGG 
TTGGACAGCG AACTCAACAT GTTCATACAC CAGTACCTGC AGCCCAGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239464.1
CDS	303..2576
Translation	MSLLFSRCNSIVTVKKDKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESAGFLEDTHRNTELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVGGTDGHEAFLLTEGSEEKKSVKTVNQLAHALHQDEQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKQFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRAQAGDRIFFVSAKEVLSARVQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHIAAQEQRVYCLEMREERQDRLRFIDKQLELLAQDYKLRIKQMTEEVERQVSTAMAEEIRRLSVLVDEYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAIASSLQTMQQDMIDGLKPLLPVSVRNQIDMLVPRQCFSLSYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALLGYNDQVQRPLPLTPANPSMPPLPQGSLTQEELMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTRAKERAFKRQFVEYASEKLQLIISYTGSNCSHQVQQELSGTFAHLCQQVDITRDNLEQEIAAMNKKVEALDSLQSKAKLLRNKAGWLDSELNMFIHQYLQPSR

Target ORF information:

RefSeq Version	XM_006239402.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239402.3

ORF Insert Sequence:

ATGTCCCTGC TCTTTTCTCG ATGCAACTCC ATCGTCACCG TCAAGAAGGA TAAGCGACAC 
ATGGCTGAGG TGAATGCTTC CCCTCTCAAG CACTTTGTCA CTGCCAAGAA AAAGATCAAT 
GGCATCTTTG AGCAGCTGGG GGCCTACATC CAAGAGAGTG CCGGCTTCCT TGAAGACACC 
CACAGGAATA CAGAACTGGA CCCAGTTACT ACAGAAGAGC AGGTCCTGGA TGTCAAAGGG 
TACCTGTCCA AGGTCAGGGG AATCAGCGAG GTGCTGGCCA GGCGGCACAT GAAGGTGGCT 
TTTTTTGGCC GGACGAGCAA TGGGAAGAGC ACCGTGATCA ATGCCATGCT TTGGGACAAA 
GTTCTGCCAT CTGGGATTGG CCACACCACC AATTGCTTCC TGCGGGTTGG GGGCACGGAT 
GGCCACGAGG CCTTCCTCCT CACGGAGGGC TCAGAAGAGA AGAAGAGTGT CAAGACCGTG 
AACCAGCTAG CCCATGCCCT CCATCAGGAT GAGCAGCTGC ATGCAGGCAG CCTGGTGAGT 
GTGATGTGGC CCAACTCCAA GTGTCCACTT CTGAAGGATG ACCTCGTGCT GATGGACAGC 
CCTGGGATCG ATGTCACCAC GGAGCTGGAC AGCTGGATTG ATAAGTTTTG CCTGGATGCT 
GATGTGTTTG TACTGGTGGC CAACTCAGAG TCCACGCTGA TGCAGACGGA GAAGCAGTTC 
TTCCACAAAG TGAGTGAGCG TCTCTCCCGG CCCAACATCT TCATCCTGAA CAACCGCTGG 
GATGCGTCTG CCTCGGAGCC TGAGTACATG GAGGAGGTGC GGCGGCAGCA CATGGAACGC 
TGCACCAGCT TTCTGGTGGA TGAGCTAGGC GTGGTGGATC GAGCTCAGGC CGGGGACCGG 
ATCTTCTTTG TGTCTGCCAA GGAGGTGCTC AGTGCCAGGG TCCAGAAAGC CCAGGGCATG 
CCAGAAGGAG GTGGCGCTCT TGCAGAAGGT TTTCAAGTGA GGATGTTTGA GTTTCAGAAT 
TTCGAGAGGC GATTTGAGGA GTGCATTTCC CAGTCTGCAG TAAAGACCAA ATTTGAGCAG 
CACACAGTCC GGGCCAAGCA GATTGCAGAG GCCGTCCGTC TCATCATGGA TTCCCTGCAC 
ATTGCGGCTC AGGAGCAGCG GGTTTATTGT CTTGAAATGC GGGAAGAGCG GCAAGACCGG 
CTGAGATTCA TTGACAAGCA GCTGGAGCTC CTGGCTCAAG ACTATAAGCT ACGGATTAAG 
CAGATGACAG AGGAAGTGGA AAGGCAGGTG TCCACAGCCA TGGCTGAAGA GATCAGGCGC 
CTCTCTGTGC TGGTTGACGA GTACCAGATG GACTTTCACC CATCCCCAGT TGTCCTCAAG 
GTTTATAAGA ATGAACTGCA CCGCCATATA GAGGAAGGCC TGGGACGAAA CATGTCTGAC 
CGCTGCTCCA CAGCCATTGC CAGTTCACTG CAGACTATGC AGCAAGACAT GATAGACGGC 
TTGAAGCCCC TTCTTCCTGT GTCTGTGCGG AATCAGATAG ACATGCTGGT CCCTCGACAG 
TGTTTCTCCC TCAGCTATGA CCTGAACTGT GACAAGCTGT GCGCTGACTT TCAGGAGGAC 
ATCGAATTCC ATTTCTCCCT TGGATGGACT ATGCTAGTGA ATAGGTTCCT GGGCCCTAAG 
AATAGCCGGC GGGCCTTGCT GGGCTACAAT GATCAGGTTC AGCGTCCTCT CCCTCTGACA 
CCTGCCAACC CCAGCATGCC ACCCTTGCCA CAGGGCTCCC TCACCCAGGA GGAGCTCATG 
GTCTCCATGG TTACTGGCCT GGCCTCTCTG ACTTCCAGGA CCTCTATGGG CATTCTCGTG 
GTCGGAGGAG TGGTGTGGAA GGCAGTGGGC TGGAGACTCA TCGCCCTCTC CTTTGGACTG 
TATGGCCTCC TGTACGTGTA TGAGCGGCTG ACCTGGACCA CCAGAGCCAA GGAGAGGGCC 
TTCAAGCGCC AGTTTGTGGA GTATGCCAGT GAGAAGCTGC AGCTCATTAT CAGTTATACC 
GGCTCTAACT GCAGCCACCA AGTTCAGCAA GAATTGTCTG GGACATTTGC TCATCTGTGC 
CAGCAAGTTG ACATCACCCG GGATAATCTG GAGCAGGAAA TTGCTGCCAT GAACAAGAAA 
GTTGAGGCTC TGGATTCACT TCAGAGCAAA GCCAAATTGC TCAGGAATAA AGCTGGCTGG 
TTGGACAGCG AACTCAACAT GTTCATACAC CAGTACCTGC AGCCCAGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfn2 ( XM_006239403.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239465.1 Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa12247
Clone ID Related Accession (Same CDS sequence)	XM_017593611.1 , XM_006239402.3 , NM_130894.4 , XM_006239403.2 , XM_008764288.2
Accession Version	XM_006239403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitofusin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239403.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGC TCTTTTCTCG ATGCAACTCC ATCGTCACCG TCAAGAAGGA TAAGCGACAC 
ATGGCTGAGG TGAATGCTTC CCCTCTCAAG CACTTTGTCA CTGCCAAGAA AAAGATCAAT 
GGCATCTTTG AGCAGCTGGG GGCCTACATC CAAGAGAGTG CCGGCTTCCT TGAAGACACC 
CACAGGAATA CAGAACTGGA CCCAGTTACT ACAGAAGAGC AGGTCCTGGA TGTCAAAGGG 
TACCTGTCCA AGGTCAGGGG AATCAGCGAG GTGCTGGCCA GGCGGCACAT GAAGGTGGCT 
TTTTTTGGCC GGACGAGCAA TGGGAAGAGC ACCGTGATCA ATGCCATGCT TTGGGACAAA 
GTTCTGCCAT CTGGGATTGG CCACACCACC AATTGCTTCC TGCGGGTTGG GGGCACGGAT 
GGCCACGAGG CCTTCCTCCT CACGGAGGGC TCAGAAGAGA AGAAGAGTGT CAAGACCGTG 
AACCAGCTAG CCCATGCCCT CCATCAGGAT GAGCAGCTGC ATGCAGGCAG CCTGGTGAGT 
GTGATGTGGC CCAACTCCAA GTGTCCACTT CTGAAGGATG ACCTCGTGCT GATGGACAGC 
CCTGGGATCG ATGTCACCAC GGAGCTGGAC AGCTGGATTG ATAAGTTTTG CCTGGATGCT 
GATGTGTTTG TACTGGTGGC CAACTCAGAG TCCACGCTGA TGCAGACGGA GAAGCAGTTC 
TTCCACAAAG TGAGTGAGCG TCTCTCCCGG CCCAACATCT TCATCCTGAA CAACCGCTGG 
GATGCGTCTG CCTCGGAGCC TGAGTACATG GAGGAGGTGC GGCGGCAGCA CATGGAACGC 
TGCACCAGCT TTCTGGTGGA TGAGCTAGGC GTGGTGGATC GAGCTCAGGC CGGGGACCGG 
ATCTTCTTTG TGTCTGCCAA GGAGGTGCTC AGTGCCAGGG TCCAGAAAGC CCAGGGCATG 
CCAGAAGGAG GTGGCGCTCT TGCAGAAGGT TTTCAAGTGA GGATGTTTGA GTTTCAGAAT 
TTCGAGAGGC GATTTGAGGA GTGCATTTCC CAGTCTGCAG TAAAGACCAA ATTTGAGCAG 
CACACAGTCC GGGCCAAGCA GATTGCAGAG GCCGTCCGTC TCATCATGGA TTCCCTGCAC 
ATTGCGGCTC AGGAGCAGCG GGTTTATTGT CTTGAAATGC GGGAAGAGCG GCAAGACCGG 
CTGAGATTCA TTGACAAGCA GCTGGAGCTC CTGGCTCAAG ACTATAAGCT ACGGATTAAG 
CAGATGACAG AGGAAGTGGA AAGGCAGGTG TCCACAGCCA TGGCTGAAGA GATCAGGCGC 
CTCTCTGTGC TGGTTGACGA GTACCAGATG GACTTTCACC CATCCCCAGT TGTCCTCAAG 
GTTTATAAGA ATGAACTGCA CCGCCATATA GAGGAAGGCC TGGGACGAAA CATGTCTGAC 
CGCTGCTCCA CAGCCATTGC CAGTTCACTG CAGACTATGC AGCAAGACAT GATAGACGGC 
TTGAAGCCCC TTCTTCCTGT GTCTGTGCGG AATCAGATAG ACATGCTGGT CCCTCGACAG 
TGTTTCTCCC TCAGCTATGA CCTGAACTGT GACAAGCTGT GCGCTGACTT TCAGGAGGAC 
ATCGAATTCC ATTTCTCCCT TGGATGGACT ATGCTAGTGA ATAGGTTCCT GGGCCCTAAG 
AATAGCCGGC GGGCCTTGCT GGGCTACAAT GATCAGGTTC AGCGTCCTCT CCCTCTGACA 
CCTGCCAACC CCAGCATGCC ACCCTTGCCA CAGGGCTCCC TCACCCAGGA GGAGCTCATG 
GTCTCCATGG TTACTGGCCT GGCCTCTCTG ACTTCCAGGA CCTCTATGGG CATTCTCGTG 
GTCGGAGGAG TGGTGTGGAA GGCAGTGGGC TGGAGACTCA TCGCCCTCTC CTTTGGACTG 
TATGGCCTCC TGTACGTGTA TGAGCGGCTG ACCTGGACCA CCAGAGCCAA GGAGAGGGCC 
TTCAAGCGCC AGTTTGTGGA GTATGCCAGT GAGAAGCTGC AGCTCATTAT CAGTTATACC 
GGCTCTAACT GCAGCCACCA AGTTCAGCAA GAATTGTCTG GGACATTTGC TCATCTGTGC 
CAGCAAGTTG ACATCACCCG GGATAATCTG GAGCAGGAAA TTGCTGCCAT GAACAAGAAA 
GTTGAGGCTC TGGATTCACT TCAGAGCAAA GCCAAATTGC TCAGGAATAA AGCTGGCTGG 
TTGGACAGCG AACTCAACAT GTTCATACAC CAGTACCTGC AGCCCAGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239465.1
CDS	333..2606
Translation	MSLLFSRCNSIVTVKKDKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESAGFLEDTHRNTELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVGGTDGHEAFLLTEGSEEKKSVKTVNQLAHALHQDEQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKQFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRAQAGDRIFFVSAKEVLSARVQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHIAAQEQRVYCLEMREERQDRLRFIDKQLELLAQDYKLRIKQMTEEVERQVSTAMAEEIRRLSVLVDEYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAIASSLQTMQQDMIDGLKPLLPVSVRNQIDMLVPRQCFSLSYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALLGYNDQVQRPLPLTPANPSMPPLPQGSLTQEELMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTRAKERAFKRQFVEYASEKLQLIISYTGSNCSHQVQQELSGTFAHLCQQVDITRDNLEQEIAAMNKKVEALDSLQSKAKLLRNKAGWLDSELNMFIHQYLQPSR

Target ORF information:

RefSeq Version	XM_006239403.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239403.2

ORF Insert Sequence:

ATGTCCCTGC TCTTTTCTCG ATGCAACTCC ATCGTCACCG TCAAGAAGGA TAAGCGACAC 
ATGGCTGAGG TGAATGCTTC CCCTCTCAAG CACTTTGTCA CTGCCAAGAA AAAGATCAAT 
GGCATCTTTG AGCAGCTGGG GGCCTACATC CAAGAGAGTG CCGGCTTCCT TGAAGACACC 
CACAGGAATA CAGAACTGGA CCCAGTTACT ACAGAAGAGC AGGTCCTGGA TGTCAAAGGG 
TACCTGTCCA AGGTCAGGGG AATCAGCGAG GTGCTGGCCA GGCGGCACAT GAAGGTGGCT 
TTTTTTGGCC GGACGAGCAA TGGGAAGAGC ACCGTGATCA ATGCCATGCT TTGGGACAAA 
GTTCTGCCAT CTGGGATTGG CCACACCACC AATTGCTTCC TGCGGGTTGG GGGCACGGAT 
GGCCACGAGG CCTTCCTCCT CACGGAGGGC TCAGAAGAGA AGAAGAGTGT CAAGACCGTG 
AACCAGCTAG CCCATGCCCT CCATCAGGAT GAGCAGCTGC ATGCAGGCAG CCTGGTGAGT 
GTGATGTGGC CCAACTCCAA GTGTCCACTT CTGAAGGATG ACCTCGTGCT GATGGACAGC 
CCTGGGATCG ATGTCACCAC GGAGCTGGAC AGCTGGATTG ATAAGTTTTG CCTGGATGCT 
GATGTGTTTG TACTGGTGGC CAACTCAGAG TCCACGCTGA TGCAGACGGA GAAGCAGTTC 
TTCCACAAAG TGAGTGAGCG TCTCTCCCGG CCCAACATCT TCATCCTGAA CAACCGCTGG 
GATGCGTCTG CCTCGGAGCC TGAGTACATG GAGGAGGTGC GGCGGCAGCA CATGGAACGC 
TGCACCAGCT TTCTGGTGGA TGAGCTAGGC GTGGTGGATC GAGCTCAGGC CGGGGACCGG 
ATCTTCTTTG TGTCTGCCAA GGAGGTGCTC AGTGCCAGGG TCCAGAAAGC CCAGGGCATG 
CCAGAAGGAG GTGGCGCTCT TGCAGAAGGT TTTCAAGTGA GGATGTTTGA GTTTCAGAAT 
TTCGAGAGGC GATTTGAGGA GTGCATTTCC CAGTCTGCAG TAAAGACCAA ATTTGAGCAG 
CACACAGTCC GGGCCAAGCA GATTGCAGAG GCCGTCCGTC TCATCATGGA TTCCCTGCAC 
ATTGCGGCTC AGGAGCAGCG GGTTTATTGT CTTGAAATGC GGGAAGAGCG GCAAGACCGG 
CTGAGATTCA TTGACAAGCA GCTGGAGCTC CTGGCTCAAG ACTATAAGCT ACGGATTAAG 
CAGATGACAG AGGAAGTGGA AAGGCAGGTG TCCACAGCCA TGGCTGAAGA GATCAGGCGC 
CTCTCTGTGC TGGTTGACGA GTACCAGATG GACTTTCACC CATCCCCAGT TGTCCTCAAG 
GTTTATAAGA ATGAACTGCA CCGCCATATA GAGGAAGGCC TGGGACGAAA CATGTCTGAC 
CGCTGCTCCA CAGCCATTGC CAGTTCACTG CAGACTATGC AGCAAGACAT GATAGACGGC 
TTGAAGCCCC TTCTTCCTGT GTCTGTGCGG AATCAGATAG ACATGCTGGT CCCTCGACAG 
TGTTTCTCCC TCAGCTATGA CCTGAACTGT GACAAGCTGT GCGCTGACTT TCAGGAGGAC 
ATCGAATTCC ATTTCTCCCT TGGATGGACT ATGCTAGTGA ATAGGTTCCT GGGCCCTAAG 
AATAGCCGGC GGGCCTTGCT GGGCTACAAT GATCAGGTTC AGCGTCCTCT CCCTCTGACA 
CCTGCCAACC CCAGCATGCC ACCCTTGCCA CAGGGCTCCC TCACCCAGGA GGAGCTCATG 
GTCTCCATGG TTACTGGCCT GGCCTCTCTG ACTTCCAGGA CCTCTATGGG CATTCTCGTG 
GTCGGAGGAG TGGTGTGGAA GGCAGTGGGC TGGAGACTCA TCGCCCTCTC CTTTGGACTG 
TATGGCCTCC TGTACGTGTA TGAGCGGCTG ACCTGGACCA CCAGAGCCAA GGAGAGGGCC 
TTCAAGCGCC AGTTTGTGGA GTATGCCAGT GAGAAGCTGC AGCTCATTAT CAGTTATACC 
GGCTCTAACT GCAGCCACCA AGTTCAGCAA GAATTGTCTG GGACATTTGC TCATCTGTGC 
CAGCAAGTTG ACATCACCCG GGATAATCTG GAGCAGGAAA TTGCTGCCAT GAACAAGAAA 
GTTGAGGCTC TGGATTCACT TCAGAGCAAA GCCAAATTGC TCAGGAATAA AGCTGGCTGG 
TTGGACAGCG AACTCAACAT GTTCATACAC CAGTACCTGC AGCCCAGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfn2 ( XM_008764288.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762510.1 Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa12247
Clone ID Related Accession (Same CDS sequence)	XM_017593611.1 , XM_006239402.3 , NM_130894.4 , XM_006239403.2 , XM_008764288.2
Accession Version	XM_008764288.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitofusin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGC TCTTTTCTCG ATGCAACTCC ATCGTCACCG TCAAGAAGGA TAAGCGACAC 
ATGGCTGAGG TGAATGCTTC CCCTCTCAAG CACTTTGTCA CTGCCAAGAA AAAGATCAAT 
GGCATCTTTG AGCAGCTGGG GGCCTACATC CAAGAGAGTG CCGGCTTCCT TGAAGACACC 
CACAGGAATA CAGAACTGGA CCCAGTTACT ACAGAAGAGC AGGTCCTGGA TGTCAAAGGG 
TACCTGTCCA AGGTCAGGGG AATCAGCGAG GTGCTGGCCA GGCGGCACAT GAAGGTGGCT 
TTTTTTGGCC GGACGAGCAA TGGGAAGAGC ACCGTGATCA ATGCCATGCT TTGGGACAAA 
GTTCTGCCAT CTGGGATTGG CCACACCACC AATTGCTTCC TGCGGGTTGG GGGCACGGAT 
GGCCACGAGG CCTTCCTCCT CACGGAGGGC TCAGAAGAGA AGAAGAGTGT CAAGACCGTG 
AACCAGCTAG CCCATGCCCT CCATCAGGAT GAGCAGCTGC ATGCAGGCAG CCTGGTGAGT 
GTGATGTGGC CCAACTCCAA GTGTCCACTT CTGAAGGATG ACCTCGTGCT GATGGACAGC 
CCTGGGATCG ATGTCACCAC GGAGCTGGAC AGCTGGATTG ATAAGTTTTG CCTGGATGCT 
GATGTGTTTG TACTGGTGGC CAACTCAGAG TCCACGCTGA TGCAGACGGA GAAGCAGTTC 
TTCCACAAAG TGAGTGAGCG TCTCTCCCGG CCCAACATCT TCATCCTGAA CAACCGCTGG 
GATGCGTCTG CCTCGGAGCC TGAGTACATG GAGGAGGTGC GGCGGCAGCA CATGGAACGC 
TGCACCAGCT TTCTGGTGGA TGAGCTAGGC GTGGTGGATC GAGCTCAGGC CGGGGACCGG 
ATCTTCTTTG TGTCTGCCAA GGAGGTGCTC AGTGCCAGGG TCCAGAAAGC CCAGGGCATG 
CCAGAAGGAG GTGGCGCTCT TGCAGAAGGT TTTCAAGTGA GGATGTTTGA GTTTCAGAAT 
TTCGAGAGGC GATTTGAGGA GTGCATTTCC CAGTCTGCAG TAAAGACCAA ATTTGAGCAG 
CACACAGTCC GGGCCAAGCA GATTGCAGAG GCCGTCCGTC TCATCATGGA TTCCCTGCAC 
ATTGCGGCTC AGGAGCAGCG GGTTTATTGT CTTGAAATGC GGGAAGAGCG GCAAGACCGG 
CTGAGATTCA TTGACAAGCA GCTGGAGCTC CTGGCTCAAG ACTATAAGCT ACGGATTAAG 
CAGATGACAG AGGAAGTGGA AAGGCAGGTG TCCACAGCCA TGGCTGAAGA GATCAGGCGC 
CTCTCTGTGC TGGTTGACGA GTACCAGATG GACTTTCACC CATCCCCAGT TGTCCTCAAG 
GTTTATAAGA ATGAACTGCA CCGCCATATA GAGGAAGGCC TGGGACGAAA CATGTCTGAC 
CGCTGCTCCA CAGCCATTGC CAGTTCACTG CAGACTATGC AGCAAGACAT GATAGACGGC 
TTGAAGCCCC TTCTTCCTGT GTCTGTGCGG AATCAGATAG ACATGCTGGT CCCTCGACAG 
TGTTTCTCCC TCAGCTATGA CCTGAACTGT GACAAGCTGT GCGCTGACTT TCAGGAGGAC 
ATCGAATTCC ATTTCTCCCT TGGATGGACT ATGCTAGTGA ATAGGTTCCT GGGCCCTAAG 
AATAGCCGGC GGGCCTTGCT GGGCTACAAT GATCAGGTTC AGCGTCCTCT CCCTCTGACA 
CCTGCCAACC CCAGCATGCC ACCCTTGCCA CAGGGCTCCC TCACCCAGGA GGAGCTCATG 
GTCTCCATGG TTACTGGCCT GGCCTCTCTG ACTTCCAGGA CCTCTATGGG CATTCTCGTG 
GTCGGAGGAG TGGTGTGGAA GGCAGTGGGC TGGAGACTCA TCGCCCTCTC CTTTGGACTG 
TATGGCCTCC TGTACGTGTA TGAGCGGCTG ACCTGGACCA CCAGAGCCAA GGAGAGGGCC 
TTCAAGCGCC AGTTTGTGGA GTATGCCAGT GAGAAGCTGC AGCTCATTAT CAGTTATACC 
GGCTCTAACT GCAGCCACCA AGTTCAGCAA GAATTGTCTG GGACATTTGC TCATCTGTGC 
CAGCAAGTTG ACATCACCCG GGATAATCTG GAGCAGGAAA TTGCTGCCAT GAACAAGAAA 
GTTGAGGCTC TGGATTCACT TCAGAGCAAA GCCAAATTGC TCAGGAATAA AGCTGGCTGG 
TTGGACAGCG AACTCAACAT GTTCATACAC CAGTACCTGC AGCCCAGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762510.1
CDS	369..2642
Translation	MSLLFSRCNSIVTVKKDKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESAGFLEDTHRNTELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVGGTDGHEAFLLTEGSEEKKSVKTVNQLAHALHQDEQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKQFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRAQAGDRIFFVSAKEVLSARVQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHIAAQEQRVYCLEMREERQDRLRFIDKQLELLAQDYKLRIKQMTEEVERQVSTAMAEEIRRLSVLVDEYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAIASSLQTMQQDMIDGLKPLLPVSVRNQIDMLVPRQCFSLSYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALLGYNDQVQRPLPLTPANPSMPPLPQGSLTQEELMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTRAKERAFKRQFVEYASEKLQLIISYTGSNCSHQVQQELSGTFAHLCQQVDITRDNLEQEIAAMNKKVEALDSLQSKAKLLRNKAGWLDSELNMFIHQYLQPSR

Target ORF information:

RefSeq Version	XM_008764288.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitofusin 2 (Mfn2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764288.2

ORF Insert Sequence:

ATGTCCCTGC TCTTTTCTCG ATGCAACTCC ATCGTCACCG TCAAGAAGGA TAAGCGACAC 
ATGGCTGAGG TGAATGCTTC CCCTCTCAAG CACTTTGTCA CTGCCAAGAA AAAGATCAAT 
GGCATCTTTG AGCAGCTGGG GGCCTACATC CAAGAGAGTG CCGGCTTCCT TGAAGACACC 
CACAGGAATA CAGAACTGGA CCCAGTTACT ACAGAAGAGC AGGTCCTGGA TGTCAAAGGG 
TACCTGTCCA AGGTCAGGGG AATCAGCGAG GTGCTGGCCA GGCGGCACAT GAAGGTGGCT 
TTTTTTGGCC GGACGAGCAA TGGGAAGAGC ACCGTGATCA ATGCCATGCT TTGGGACAAA 
GTTCTGCCAT CTGGGATTGG CCACACCACC AATTGCTTCC TGCGGGTTGG GGGCACGGAT 
GGCCACGAGG CCTTCCTCCT CACGGAGGGC TCAGAAGAGA AGAAGAGTGT CAAGACCGTG 
AACCAGCTAG CCCATGCCCT CCATCAGGAT GAGCAGCTGC ATGCAGGCAG CCTGGTGAGT 
GTGATGTGGC CCAACTCCAA GTGTCCACTT CTGAAGGATG ACCTCGTGCT GATGGACAGC 
CCTGGGATCG ATGTCACCAC GGAGCTGGAC AGCTGGATTG ATAAGTTTTG CCTGGATGCT 
GATGTGTTTG TACTGGTGGC CAACTCAGAG TCCACGCTGA TGCAGACGGA GAAGCAGTTC 
TTCCACAAAG TGAGTGAGCG TCTCTCCCGG CCCAACATCT TCATCCTGAA CAACCGCTGG 
GATGCGTCTG CCTCGGAGCC TGAGTACATG GAGGAGGTGC GGCGGCAGCA CATGGAACGC 
TGCACCAGCT TTCTGGTGGA TGAGCTAGGC GTGGTGGATC GAGCTCAGGC CGGGGACCGG 
ATCTTCTTTG TGTCTGCCAA GGAGGTGCTC AGTGCCAGGG TCCAGAAAGC CCAGGGCATG 
CCAGAAGGAG GTGGCGCTCT TGCAGAAGGT TTTCAAGTGA GGATGTTTGA GTTTCAGAAT 
TTCGAGAGGC GATTTGAGGA GTGCATTTCC CAGTCTGCAG TAAAGACCAA ATTTGAGCAG 
CACACAGTCC GGGCCAAGCA GATTGCAGAG GCCGTCCGTC TCATCATGGA TTCCCTGCAC 
ATTGCGGCTC AGGAGCAGCG GGTTTATTGT CTTGAAATGC GGGAAGAGCG GCAAGACCGG 
CTGAGATTCA TTGACAAGCA GCTGGAGCTC CTGGCTCAAG ACTATAAGCT ACGGATTAAG 
CAGATGACAG AGGAAGTGGA AAGGCAGGTG TCCACAGCCA TGGCTGAAGA GATCAGGCGC 
CTCTCTGTGC TGGTTGACGA GTACCAGATG GACTTTCACC CATCCCCAGT TGTCCTCAAG 
GTTTATAAGA ATGAACTGCA CCGCCATATA GAGGAAGGCC TGGGACGAAA CATGTCTGAC 
CGCTGCTCCA CAGCCATTGC CAGTTCACTG CAGACTATGC AGCAAGACAT GATAGACGGC 
TTGAAGCCCC TTCTTCCTGT GTCTGTGCGG AATCAGATAG ACATGCTGGT CCCTCGACAG 
TGTTTCTCCC TCAGCTATGA CCTGAACTGT GACAAGCTGT GCGCTGACTT TCAGGAGGAC 
ATCGAATTCC ATTTCTCCCT TGGATGGACT ATGCTAGTGA ATAGGTTCCT GGGCCCTAAG 
AATAGCCGGC GGGCCTTGCT GGGCTACAAT GATCAGGTTC AGCGTCCTCT CCCTCTGACA 
CCTGCCAACC CCAGCATGCC ACCCTTGCCA CAGGGCTCCC TCACCCAGGA GGAGCTCATG 
GTCTCCATGG TTACTGGCCT GGCCTCTCTG ACTTCCAGGA CCTCTATGGG CATTCTCGTG 
GTCGGAGGAG TGGTGTGGAA GGCAGTGGGC TGGAGACTCA TCGCCCTCTC CTTTGGACTG 
TATGGCCTCC TGTACGTGTA TGAGCGGCTG ACCTGGACCA CCAGAGCCAA GGAGAGGGCC 
TTCAAGCGCC AGTTTGTGGA GTATGCCAGT GAGAAGCTGC AGCTCATTAT CAGTTATACC 
GGCTCTAACT GCAGCCACCA AGTTCAGCAA GAATTGTCTG GGACATTTGC TCATCTGTGC 
CAGCAAGTTG ACATCACCCG GGATAATCTG GAGCAGGAAA TTGCTGCCAT GAACAAGAAA 
GTTGAGGCTC TGGATTCACT TCAGAGCAAA GCCAAATTGC TCAGGAATAA AGCTGGCTGG 
TTGGACAGCG AACTCAACAT GTTCATACAC CAGTACCTGC AGCCCAGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitofusin-2 mediated mitochondrial Ca
World journal of gastroenterology23(38)6995-7008(2017 Oct)
Liang RP,Jia JJ,Li JH,He N,Zhou YF,Jiang L,Bai T,Xie HY,Zhou L,Sun YL

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Mfn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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