P4ha1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	P4ha1
Entrez Gene ID	64475
Full Name	prolyl 4-hydroxylase subunit alpha 1
Synonyms	PHalphaI
General protein information	Preferred Names prolyl 4-hydroxylase subunit alpha 1 Names prolyl 4-hydroxylase subunit alpha-1 4-PH alpha-1 procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1 prolyl 4-hydroxylase alpha subunit prolyl 4-hydroxylase, alpha polypeptide I
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11
Summary	catalyzes the hydroxylation of -X-Pro-Gly- triplets to form hydroxyproline [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256425.2	XP_006256487.1	prolyl 4-hydroxylase subunit alpha-1 isoform X1
XM_006256424.3	XP_006256486.1	prolyl 4-hydroxylase subunit alpha-1 isoform X2
NM_172062.2	NP_742059.2	prolyl 4-hydroxylase subunit alpha-1 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	P4HA1	XP_005619064.1
Bos taurus (cattle)	P4HA1	NP_001069238.1
Danio rerio (zebrafish)	p4ha1a	NP_001038463.1
Drosophila melanogaster (fruit fly)	PH4alphaEFB	NP_733371.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000971	XP_309164.4
Rattus norvegicus (Norway rat)	P4ha1	NP_742059.2
Caenorhabditis elegans (roundworm)	dpy-18	NP_499464.1
Xenopus tropicalis (tropical clawed frog)	LOC100490484	XP_002936004.1
	P4HA1	XP_001103971.2
Xenopus tropicalis (tropical clawed frog)	p4ha1	XP_004915844.1
Homo sapiens (human)	P4HA1	NP_001136067.1
Mus musculus (house mouse)	P4ha1	NP_035160.1
Danio rerio (zebrafish)	LOC556879	XP_005156147.1
Caenorhabditis elegans (roundworm)	phy-2	NP_502317.1
Pan troglodytes (chimpanzee)	P4HA1	XP_001140652.1
Gallus gallus (chicken)	P4HA1	NP_001185667.1

Gene Ontology
Bibliography

Related articles in PubMed

The structural motifs for substrate binding and dimerization of the ? subunit of collagen prolyl 4-hydroxylase.
Anantharajan J, Koski MK, Kursula P, Hieta R, Bergmann U, Myllyharju J, Wierenga RK
Structure (London, England : 1993)21(12)2107-18(2013 Dec)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Loss of assembly of the main basement membrane collagen, type IV, but not fibril-forming collagens and embryonic death in collagen prolyl 4-hydroxylase I null mice.
Holster T, Pakkanen O, Soininen R, Sormunen R, Nokelainen M, Kivirikko KI, Myllyharju J
The Journal of biological chemistry282(4)2512-9(2007 Jan)

Cloning and characterization of the 5'-flanking region of the rat P4Halpha gene encoding the prolyl 4-hydroxylase alpha(I) subunit.
Takahashi S, Dohi N, Takahashi Y, Miura T
Biochimica et biophysica acta1574(3)354-8(2002 Apr)

Hypoxic induction of prolyl 4-hydroxylase alpha (I) in cultured cells.
Takahashi Y, Takahashi S, Shiga Y, Yoshimi T, Miura T
The Journal of biological chemistry275(19)14139-46(2000 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following P4ha1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P4ha1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06015	NM_172062.2 Latest version!	Rattus norvegicus prolyl 4-hydroxylase subunit alpha 1 (P4ha1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa06015	XM_006256424.3 Latest version!	Rattus norvegicus prolyl 4-hydroxylase subunit alpha 1 (P4ha1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa39449	XM_006256425.2 Latest version!	Rattus norvegicus prolyl 4-hydroxylase subunit alpha 1 (P4ha1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P4ha1 ( NM_172062.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742059.2 Rattus norvegicus prolyl 4-hydroxylase subunit alpha 1 (P4ha1), mRNA.

Price & Availability↑

CloneID	ORa06015
Clone ID Related Accession (Same CDS sequence)	XM_006256424.3 , NM_172062.2
Accession Version	NM_172062.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	prolyl 4-hydroxylase subunit alpha-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078703.1. On Aug 8, 2004 this sequence version replaced NM_172062.1. ##Evidence-Data-START## Transcript exon combination :: BC078703.1, X78949.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGATCTGGG GTGTATTAAT GATGGGGATT CTACTTCCTC AGTGTTCAGC CCATCCAGGC 
TTTTTTACTT CAATTGGTCA GATGACTGAC TTGATCCATA ATGAGAAAGA CCTGGTGACG 
TCACTAAAAG ATTACATTAA AGCAGAAGAG GACAAGTTAG AGCAAATCAA AAAATGGGCA 
GAGAAGTTAG ACCGGCTAAC AAGTACAGCA ACAAAAGATC CAGAAGGGTT TGTCGGACAC 
CCTGTAAATG CATTCAAGTT AATGAAACGT CTGAACACCG AGTGGAGTGA GTTGGAGAAT 
CTGATCCTCA AGGATATGTC AGATGGCTTC ATCTCTAACC TGACCATTCA GAGGCAGTAC 
TTCCCTAACG ACGAAGACCA GGTTGGGGCT GCAAAAGCTT TGTTTCGTCT GCAAGACACC 
TACAACCTAG ACACGAATAC CATCTCGAAG GGCAATCTTC CAGGAGTGAA ACACAAGTCT 
TTTCTAACAG CTGAGGACTG CTTTGAGTTG GGCAAAGTGG CCTATACAGA AGCAGATTAT 
TACCACACAG AACTCTGGAT GGAGCAGGCT CTGATGCAGC TGGAGGAGGG AGAGATGTCT 
ACTGTAGACA AAGTCTCGGT TCTAGATTAT TTGAGCTATG CAGTGTACCA GCAGGGTGAC 
CTGGATAAGG CACTTCTGCT TACAAAGAAA CTTCTTGAAC TAGATCCTGA ACACCAGAGA 
GCCAATGGTA ACTTAGTATA TTTTGAGTAT ATAATGAGTA AAGAAAAAGA TGCCAATAAG 
TCTGCTTCGG GTGACCAGTC TGATCAGAAA ACTACACCAA AGAAAAAGGG GATTGCTGTG 
GACTACCTGC CAGAGAGACA GAAGTACGAA ATGCTGTGCC GTGGGGAGGG TATCAAAATG 
ACTCCTCGGA GACAAAAAAG GCTGTTCTGC CGCTACCATG ATGGAAACCG GAATCCTAAA 
TTTATCCTGG CCCCAGCCAA GCAGGAGGAT GAGTGGGACA AGCCTCGCAT CATTCGTTTC 
CATGACATCA TCTCAGATGC CGAGATTGAG ATCGTCAAAG ATTTAGCAAA GCCCAGGCTG 
AGCCGAGCTA CAGTACATGA CCCTGAGACT GGGAAATTGA CCACAGCACA GTACAGAGTA 
TCTAAGAGTG CTTGGCTGTC TGGCTATGAA GATCCTGTGG TGTCTCGAAT TAATATGAGA 
ATACAAGATC TCACAGGACT GGATGTTTCC ACGGCAGAGG AATTACAGGT AGCAAATTAT 
GGAGTTGGAG GACAGTATGA ACCCCATTTT GACTTTGCCA GGAAAGACGA GCCGGATGCT 
TTTAGAGAGC TTGGGACAGG AAATAGGATT GCCACGTGGC TCTTCTACAT GAGTGATGTG 
TCTGCTGGAG GCGCTACTGT TTTTCCTGAA GTGGGAGCCA GTGTTTGGCC CAAAAAAGGC 
ACTGCTGTCT TCTGGTACAA TCTGTTTGCC AGTGGAGAAG GAGATTACAG TACACGGCAC 
GCAGCCTGTC CTGTGCTAGT GGGAAACAAA TGGGTATCCA ACAAATGGCT CCATGAACGT 
GGACAGGAAT TTCGAAGGCC GTGTACCCTG TCAGAATTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742059.2
CDS	236..1840
Translation	MIWGVLMMGILLPQCSAHPGFFTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALMQLEEGEMSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTTPKKKGIAVDYLPERQKYEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLSRATVHDPETGKLTTAQYRVSKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Target ORF information:

RefSeq Version	NM_172062.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prolyl 4-hydroxylase subunit alpha 1 (P4ha1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172062.2

ORF Insert Sequence:

ATGATCTGGG GTGTATTAAT GATGGGGATT CTACTTCCTC AGTGTTCAGC CCATCCAGGC 
TTTTTTACTT CAATTGGTCA GATGACTGAC TTGATCCATA ATGAGAAAGA CCTGGTGACG 
TCACTAAAAG ATTACATTAA AGCAGAAGAG GACAAGTTAG AGCAAATCAA AAAATGGGCA 
GAGAAGTTAG ACCGGCTAAC AAGTACAGCA ACAAAAGATC CAGAAGGGTT TGTCGGACAC 
CCTGTAAATG CATTCAAGTT AATGAAACGT CTGAACACCG AGTGGAGTGA GTTGGAGAAT 
CTGATCCTCA AGGATATGTC AGATGGCTTC ATCTCTAACC TGACCATTCA GAGGCAGTAC 
TTCCCTAACG ACGAAGACCA GGTTGGGGCT GCAAAAGCTT TGTTTCGTCT GCAAGACACC 
TACAACCTAG ACACGAATAC CATCTCGAAG GGCAATCTTC CAGGAGTGAA ACACAAGTCT 
TTTCTAACAG CTGAGGACTG CTTTGAGTTG GGCAAAGTGG CCTATACAGA AGCAGATTAT 
TACCACACAG AACTCTGGAT GGAGCAGGCT CTGATGCAGC TGGAGGAGGG AGAGATGTCT 
ACTGTAGACA AAGTCTCGGT TCTAGATTAT TTGAGCTATG CAGTGTACCA GCAGGGTGAC 
CTGGATAAGG CACTTCTGCT TACAAAGAAA CTTCTTGAAC TAGATCCTGA ACACCAGAGA 
GCCAATGGTA ACTTAGTATA TTTTGAGTAT ATAATGAGTA AAGAAAAAGA TGCCAATAAG 
TCTGCTTCGG GTGACCAGTC TGATCAGAAA ACTACACCAA AGAAAAAGGG GATTGCTGTG 
GACTACCTGC CAGAGAGACA GAAGTACGAA ATGCTGTGCC GTGGGGAGGG TATCAAAATG 
ACTCCTCGGA GACAAAAAAG GCTGTTCTGC CGCTACCATG ATGGAAACCG GAATCCTAAA 
TTTATCCTGG CCCCAGCCAA GCAGGAGGAT GAGTGGGACA AGCCTCGCAT CATTCGTTTC 
CATGACATCA TCTCAGATGC CGAGATTGAG ATCGTCAAAG ATTTAGCAAA GCCCAGGCTG 
AGCCGAGCTA CAGTACATGA CCCTGAGACT GGGAAATTGA CCACAGCACA GTACAGAGTA 
TCTAAGAGTG CTTGGCTGTC TGGCTATGAA GATCCTGTGG TGTCTCGAAT TAATATGAGA 
ATACAAGATC TCACAGGACT GGATGTTTCC ACGGCAGAGG AATTACAGGT AGCAAATTAT 
GGAGTTGGAG GACAGTATGA ACCCCATTTT GACTTTGCCA GGAAAGACGA GCCGGATGCT 
TTTAGAGAGC TTGGGACAGG AAATAGGATT GCCACGTGGC TCTTCTACAT GAGTGATGTG 
TCTGCTGGAG GCGCTACTGT TTTTCCTGAA GTGGGAGCCA GTGTTTGGCC CAAAAAAGGC 
ACTGCTGTCT TCTGGTACAA TCTGTTTGCC AGTGGAGAAG GAGATTACAG TACACGGCAC 
GCAGCCTGTC CTGTGCTAGT GGGAAACAAA TGGGTATCCA ACAAATGGCT CCATGAACGT 
GGACAGGAAT TTCGAAGGCC GTGTACCCTG TCAGAATTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4ha1 ( XM_006256424.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256486.1 Rattus norvegicus prolyl 4-hydroxylase subunit alpha 1 (P4ha1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa06015
Clone ID Related Accession (Same CDS sequence)	XM_006256424.3 , NM_172062.2
Accession Version	XM_006256424.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256424.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGGG GTGTATTAAT GATGGGGATT CTACTTCCTC AGTGTTCAGC CCATCCAGGC 
TTTTTTACTT CAATTGGTCA GATGACTGAC TTGATCCATA ATGAGAAAGA CCTGGTGACG 
TCACTAAAAG ATTACATTAA AGCAGAAGAG GACAAGTTAG AGCAAATCAA AAAATGGGCA 
GAGAAGTTAG ACCGGCTAAC AAGTACAGCA ACAAAAGATC CAGAAGGGTT TGTCGGACAC 
CCTGTAAATG CATTCAAGTT AATGAAACGT CTGAACACCG AGTGGAGTGA GTTGGAGAAT 
CTGATCCTCA AGGATATGTC AGATGGCTTC ATCTCTAACC TGACCATTCA GAGGCAGTAC 
TTCCCTAACG ACGAAGACCA GGTTGGGGCT GCAAAAGCTT TGTTTCGTCT GCAAGACACC 
TACAACCTAG ACACGAATAC CATCTCGAAG GGCAATCTTC CAGGAGTGAA ACACAAGTCT 
TTTCTAACAG CTGAGGACTG CTTTGAGTTG GGCAAAGTGG CCTATACAGA AGCAGATTAT 
TACCACACAG AACTCTGGAT GGAGCAGGCT CTGATGCAGC TGGAGGAGGG AGAGATGTCT 
ACTGTAGACA AAGTCTCGGT TCTAGATTAT TTGAGCTATG CAGTGTACCA GCAGGGTGAC 
CTGGATAAGG CACTTCTGCT TACAAAGAAA CTTCTTGAAC TAGATCCTGA ACACCAGAGA 
GCCAATGGTA ACTTAGTATA TTTTGAGTAT ATAATGAGTA AAGAAAAAGA TGCCAATAAG 
TCTGCTTCGG GTGACCAGTC TGATCAGAAA ACTACACCAA AGAAAAAGGG GATTGCTGTG 
GACTACCTGC CAGAGAGACA GAAGTACGAA ATGCTGTGCC GTGGGGAGGG TATCAAAATG 
ACTCCTCGGA GACAAAAAAG GCTGTTCTGC CGCTACCATG ATGGAAACCG GAATCCTAAA 
TTTATCCTGG CCCCAGCCAA GCAGGAGGAT GAGTGGGACA AGCCTCGCAT CATTCGTTTC 
CATGACATCA TCTCAGATGC CGAGATTGAG ATCGTCAAAG ATTTAGCAAA GCCCAGGCTG 
AGCCGAGCTA CAGTACATGA CCCTGAGACT GGGAAATTGA CCACAGCACA GTACAGAGTA 
TCTAAGAGTG CTTGGCTGTC TGGCTATGAA GATCCTGTGG TGTCTCGAAT TAATATGAGA 
ATACAAGATC TCACAGGACT GGATGTTTCC ACGGCAGAGG AATTACAGGT AGCAAATTAT 
GGAGTTGGAG GACAGTATGA ACCCCATTTT GACTTTGCCA GGAAAGACGA GCCGGATGCT 
TTTAGAGAGC TTGGGACAGG AAATAGGATT GCCACGTGGC TCTTCTACAT GAGTGATGTG 
TCTGCTGGAG GCGCTACTGT TTTTCCTGAA GTGGGAGCCA GTGTTTGGCC CAAAAAAGGC 
ACTGCTGTCT TCTGGTACAA TCTGTTTGCC AGTGGAGAAG GAGATTACAG TACACGGCAC 
GCAGCCTGTC CTGTGCTAGT GGGAAACAAA TGGGTATCCA ACAAATGGCT CCATGAACGT 
GGACAGGAAT TTCGAAGGCC GTGTACCCTG TCAGAATTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256486.1
CDS	324..1928
Translation	MIWGVLMMGILLPQCSAHPGFFTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALMQLEEGEMSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTTPKKKGIAVDYLPERQKYEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLSRATVHDPETGKLTTAQYRVSKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Target ORF information:

RefSeq Version	XM_006256424.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prolyl 4-hydroxylase subunit alpha 1 (P4ha1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256424.3

ORF Insert Sequence:

ATGATCTGGG GTGTATTAAT GATGGGGATT CTACTTCCTC AGTGTTCAGC CCATCCAGGC 
TTTTTTACTT CAATTGGTCA GATGACTGAC TTGATCCATA ATGAGAAAGA CCTGGTGACG 
TCACTAAAAG ATTACATTAA AGCAGAAGAG GACAAGTTAG AGCAAATCAA AAAATGGGCA 
GAGAAGTTAG ACCGGCTAAC AAGTACAGCA ACAAAAGATC CAGAAGGGTT TGTCGGACAC 
CCTGTAAATG CATTCAAGTT AATGAAACGT CTGAACACCG AGTGGAGTGA GTTGGAGAAT 
CTGATCCTCA AGGATATGTC AGATGGCTTC ATCTCTAACC TGACCATTCA GAGGCAGTAC 
TTCCCTAACG ACGAAGACCA GGTTGGGGCT GCAAAAGCTT TGTTTCGTCT GCAAGACACC 
TACAACCTAG ACACGAATAC CATCTCGAAG GGCAATCTTC CAGGAGTGAA ACACAAGTCT 
TTTCTAACAG CTGAGGACTG CTTTGAGTTG GGCAAAGTGG CCTATACAGA AGCAGATTAT 
TACCACACAG AACTCTGGAT GGAGCAGGCT CTGATGCAGC TGGAGGAGGG AGAGATGTCT 
ACTGTAGACA AAGTCTCGGT TCTAGATTAT TTGAGCTATG CAGTGTACCA GCAGGGTGAC 
CTGGATAAGG CACTTCTGCT TACAAAGAAA CTTCTTGAAC TAGATCCTGA ACACCAGAGA 
GCCAATGGTA ACTTAGTATA TTTTGAGTAT ATAATGAGTA AAGAAAAAGA TGCCAATAAG 
TCTGCTTCGG GTGACCAGTC TGATCAGAAA ACTACACCAA AGAAAAAGGG GATTGCTGTG 
GACTACCTGC CAGAGAGACA GAAGTACGAA ATGCTGTGCC GTGGGGAGGG TATCAAAATG 
ACTCCTCGGA GACAAAAAAG GCTGTTCTGC CGCTACCATG ATGGAAACCG GAATCCTAAA 
TTTATCCTGG CCCCAGCCAA GCAGGAGGAT GAGTGGGACA AGCCTCGCAT CATTCGTTTC 
CATGACATCA TCTCAGATGC CGAGATTGAG ATCGTCAAAG ATTTAGCAAA GCCCAGGCTG 
AGCCGAGCTA CAGTACATGA CCCTGAGACT GGGAAATTGA CCACAGCACA GTACAGAGTA 
TCTAAGAGTG CTTGGCTGTC TGGCTATGAA GATCCTGTGG TGTCTCGAAT TAATATGAGA 
ATACAAGATC TCACAGGACT GGATGTTTCC ACGGCAGAGG AATTACAGGT AGCAAATTAT 
GGAGTTGGAG GACAGTATGA ACCCCATTTT GACTTTGCCA GGAAAGACGA GCCGGATGCT 
TTTAGAGAGC TTGGGACAGG AAATAGGATT GCCACGTGGC TCTTCTACAT GAGTGATGTG 
TCTGCTGGAG GCGCTACTGT TTTTCCTGAA GTGGGAGCCA GTGTTTGGCC CAAAAAAGGC 
ACTGCTGTCT TCTGGTACAA TCTGTTTGCC AGTGGAGAAG GAGATTACAG TACACGGCAC 
GCAGCCTGTC CTGTGCTAGT GGGAAACAAA TGGGTATCCA ACAAATGGCT CCATGAACGT 
GGACAGGAAT TTCGAAGGCC GTGTACCCTG TCAGAATTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4ha1 ( XM_006256425.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256487.1 Rattus norvegicus prolyl 4-hydroxylase subunit alpha 1 (P4ha1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39449
Clone ID Related Accession (Same CDS sequence)	XM_006256425.2
Accession Version	XM_006256425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006256425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGGG GTGTATTAAT GATGGGGATT CTACTTCCTC AGTGTTCAGC CCATCCAGGC 
TTTTTTACTT CAATTGGTCA GATGACTGAC TTGATCCATA ATGAGAAAGA CCTGGTGACG 
TCACTAAAAG ATTACATTAA AGCAGAAGAG GACAAGTTAG AGCAAATCAA AAAATGGGCA 
GAGAAGTTAG ACCGGCTAAC AAGTACAGCA ACAAAAGATC CAGAAGGGTT TGTCGGACAC 
CCTGTAAATG CATTCAAGTT AATGAAACGT CTGAACACCG AGTGGAGTGA GTTGGAGAAT 
CTGATCCTCA AGGATATGTC AGATGGCTTC ATCTCTAACC TGACCATTCA GAGGCAGTAC 
TTCCCTAACG ACGAAGACCA GGTTGGGGCT GCAAAAGCTT TGTTTCGTCT GCAAGACACC 
TACAACCTAG ACACGAATAC CATCTCGAAG GGCAATCTTC CAGGAGTGAA ACACAAGTCT 
TTTCTAACAG CTGAGGACTG CTTTGAGTTG GGCAAAGTGG CCTATACAGA AGCAGATTAT 
TACCACACAG AACTCTGGAT GGAGCAGGCT CTGATGCAGC TGGAGGAGGG AGAGATGTCT 
ACTGTAGACA AAGTCTCGGT TCTAGATTAT TTGAGCTATG CAGTGTACCA GCAGGGTGAC 
CTGGATAAGG CACTTCTGCT TACAAAGAAA CTTCTTGAAC TAGATCCTGA ACACCAGAGA 
GCCAATGGTA ACTTAGTATA TTTTGAGTAT ATAATGAGTA AAGAAAAAGA TGCCAATAAG 
TCTGCTTCGG GTGACCAGTC TGATCAGAAA ACTACACCAA AGAAAAAGGG GATTGCTGTG 
GACTACCTGC CAGAGAGACA GAAGTACGAA ATGCTGTGCC GTGGGGAGGG TATCAAAATG 
ACTCCTCGGA GACAAAAAAG GCTGTTCTGC CGCTACCATG ATGGAAACCG GAATCCTAAA 
TTTATCCTGG CCCCAGCCAA GCAGGAGGAT GAGTGGGACA AGCCTCGCAT CATTCGTTTC 
CATGACATCA TCTCAGATGC CGAGATTGAG ATCGTCAAAG ATTTAGCAAA GCCCAGGCTG 
AGGCGAGCCA CCATTTCAAA CCCAGTAACA GGAGCCTTGG AGACGGTACA TTACAGAATT 
AGCAAAAGTG CTTGGCTGTC TGGCTATGAA GATCCTGTGG TGTCTCGAAT TAATATGAGA 
ATACAAGATC TCACAGGACT GGATGTTTCC ACGGCAGAGG AATTACAGGT AGCAAATTAT 
GGAGTTGGAG GACAGTATGA ACCCCATTTT GACTTTGCCA GGAAAGACGA GCCGGATGCT 
TTTAGAGAGC TTGGGACAGG AAATAGGATT GCCACGTGGC TCTTCTACAT GAGTGATGTG 
TCTGCTGGAG GCGCTACTGT TTTTCCTGAA GTGGGAGCCA GTGTTTGGCC CAAAAAAGGC 
ACTGCTGTCT TCTGGTACAA TCTGTTTGCC AGTGGAGAAG GAGATTACAG TACACGGCAC 
GCAGCCTGTC CTGTGCTAGT GGGAAACAAA TGGGTATCCA ACAAATGGCT CCATGAACGT 
GGACAGGAAT TTCGAAGGCC GTGTACCCTG TCAGAATTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256487.1
CDS	230..1834
Translation	MIWGVLMMGILLPQCSAHPGFFTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALMQLEEGEMSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTTPKKKGIAVDYLPERQKYEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPVTGALETVHYRISKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Target ORF information:

RefSeq Version	XM_006256425.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prolyl 4-hydroxylase subunit alpha 1 (P4ha1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256425.2

ORF Insert Sequence:

ATGATCTGGG GTGTATTAAT GATGGGGATT CTACTTCCTC AGTGTTCAGC CCATCCAGGC 
TTTTTTACTT CAATTGGTCA GATGACTGAC TTGATCCATA ATGAGAAAGA CCTGGTGACG 
TCACTAAAAG ATTACATTAA AGCAGAAGAG GACAAGTTAG AGCAAATCAA AAAATGGGCA 
GAGAAGTTAG ACCGGCTAAC AAGTACAGCA ACAAAAGATC CAGAAGGGTT TGTCGGACAC 
CCTGTAAATG CATTCAAGTT AATGAAACGT CTGAACACCG AGTGGAGTGA GTTGGAGAAT 
CTGATCCTCA AGGATATGTC AGATGGCTTC ATCTCTAACC TGACCATTCA GAGGCAGTAC 
TTCCCTAACG ACGAAGACCA GGTTGGGGCT GCAAAAGCTT TGTTTCGTCT GCAAGACACC 
TACAACCTAG ACACGAATAC CATCTCGAAG GGCAATCTTC CAGGAGTGAA ACACAAGTCT 
TTTCTAACAG CTGAGGACTG CTTTGAGTTG GGCAAAGTGG CCTATACAGA AGCAGATTAT 
TACCACACAG AACTCTGGAT GGAGCAGGCT CTGATGCAGC TGGAGGAGGG AGAGATGTCT 
ACTGTAGACA AAGTCTCGGT TCTAGATTAT TTGAGCTATG CAGTGTACCA GCAGGGTGAC 
CTGGATAAGG CACTTCTGCT TACAAAGAAA CTTCTTGAAC TAGATCCTGA ACACCAGAGA 
GCCAATGGTA ACTTAGTATA TTTTGAGTAT ATAATGAGTA AAGAAAAAGA TGCCAATAAG 
TCTGCTTCGG GTGACCAGTC TGATCAGAAA ACTACACCAA AGAAAAAGGG GATTGCTGTG 
GACTACCTGC CAGAGAGACA GAAGTACGAA ATGCTGTGCC GTGGGGAGGG TATCAAAATG 
ACTCCTCGGA GACAAAAAAG GCTGTTCTGC CGCTACCATG ATGGAAACCG GAATCCTAAA 
TTTATCCTGG CCCCAGCCAA GCAGGAGGAT GAGTGGGACA AGCCTCGCAT CATTCGTTTC 
CATGACATCA TCTCAGATGC CGAGATTGAG ATCGTCAAAG ATTTAGCAAA GCCCAGGCTG 
AGGCGAGCCA CCATTTCAAA CCCAGTAACA GGAGCCTTGG AGACGGTACA TTACAGAATT 
AGCAAAAGTG CTTGGCTGTC TGGCTATGAA GATCCTGTGG TGTCTCGAAT TAATATGAGA 
ATACAAGATC TCACAGGACT GGATGTTTCC ACGGCAGAGG AATTACAGGT AGCAAATTAT 
GGAGTTGGAG GACAGTATGA ACCCCATTTT GACTTTGCCA GGAAAGACGA GCCGGATGCT 
TTTAGAGAGC TTGGGACAGG AAATAGGATT GCCACGTGGC TCTTCTACAT GAGTGATGTG 
TCTGCTGGAG GCGCTACTGT TTTTCCTGAA GTGGGAGCCA GTGTTTGGCC CAAAAAAGGC 
ACTGCTGTCT TCTGGTACAA TCTGTTTGCC AGTGGAGAAG GAGATTACAG TACACGGCAC 
GCAGCCTGTC CTGTGCTAGT GGGAAACAAA TGGGTATCCA ACAAATGGCT CCATGAACGT 
GGACAGGAAT TTCGAAGGCC GTGTACCCTG TCAGAATTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The structural motifs for substrate binding and dimerization of the ? subunit of collagen prolyl 4-hydroxylase.
Structure (London, England : 1993)21(12)2107-18(2013 Dec)
Anantharajan J,Koski MK,Kursula P,Hieta R,Bergmann U,Myllyharju J,Wierenga RK

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Loss of assembly of the main basement membrane collagen, type IV, but not fibril-forming collagens and embryonic death in collagen prolyl 4-hydroxylase I null mice.
The Journal of biological chemistry282(4)2512-9(2007 Jan)
Holster T,Pakkanen O,Soininen R,Sormunen R,Nokelainen M,Kivirikko KI,Myllyharju J

Cloning and characterization of the 5'-flanking region of the rat P4Halpha gene encoding the prolyl 4-hydroxylase alpha(I) subunit.
Biochimica et biophysica acta1574(3)354-8(2002 Apr)
Takahashi S,Dohi N,Takahashi Y,Miura T

Hypoxic induction of prolyl 4-hydroxylase alpha (I) in cultured cells.
The Journal of biological chemistry275(19)14139-46(2000 May)
Takahashi Y,Takahashi S,Shiga Y,Yoshimi T,Miura T

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P4ha1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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