Spon1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spon1
Entrez Gene ID	64456
Full Name	spondin 1
Synonyms	Sponf
General protein information	Preferred Names spondin 1 Names spondin-1 f-spondin spondin 1, (f-spondin) extracellular matrix protein spondin 1, extracellular matrix protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q33
Summary	promotes cell attachment; may play a role in axon guidance in the spinal cord and peripheral nervous system [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_172067.1	NP_742064.1	spondin-1 precursor
XM_017589664.1	XP_017445153.1	spondin-1 isoform X1

Pathways from BioSystems

Homologies

	SPON1	XP_001093336.1
Mus musculus (house mouse)	Spon1	NP_663559.1
Xenopus tropicalis (tropical clawed frog)	spon1	NP_001090741.1
Homo sapiens (human)	SPON1	NP_006099.2
Rattus norvegicus (Norway rat)	Spon1	NP_742064.1
Gallus gallus (chicken)	SPON1	NP_990182.1
Danio rerio (zebrafish)	spon1a	NP_571590.1
Drosophila melanogaster (fruit fly)	CG17739	NP_610757.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012307	XP_320234.3
Pan troglodytes (chimpanzee)	SPON1	XP_508295.3
Canis lupus familiaris (dog)	SPON1	XP_534067.2
Bos taurus (cattle)	SPON1	NP_777168.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteomics characterization of extracellular space components in the human aorta.
Didangelos A, Yin X, Mandal K, Baumert M, Jahangiri M, Mayr M
Molecular & cellular proteomics : MCP9(9)2048-62(2010 Sep)

Genetic dissection of a blood pressure quantitative trait locus on rat chromosome 1 and gene expression analysis identifies SPON1 as a novel candidate hypertension gene.
Clemitson JR, Dixon RJ, Haines S, Bingham AJ, Patel BR, Hall L, Lo M, Sassard J, Charchar FJ, Samani NJ
Circulation research100(7)992-9(2007 Apr)

F-spondin interaction with the apolipoprotein E receptor ApoEr2 affects processing of amyloid precursor protein.
Hoe HS, Wessner D, Beffert U, Becker AG, Matsuoka Y, Rebeck GW
Molecular and cellular biology25(21)9259-68(2005 Nov)

Binding of F-spondin to amyloid-beta precursor protein: a candidate amyloid-beta precursor protein ligand that modulates amyloid-beta precursor protein cleavage.
Ho A, S?dhof TC
Proceedings of the National Academy of Sciences of the United States of America101(8)2548-53(2004 Feb)

F-spondin and mindin: two structurally and functionally related genes expressed in the hippocampus that promote outgrowth of embryonic hippocampal neurons.
Feinstein Y, Borrell V, Garcia C, Burstyn-Cohen T, Tzarfaty V, Frumkin A, Nose A, Okamoto H, Higashijima S, Soriano E, Klar A
Development (Cambridge, England)126(16)3637-48(1999 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings justify further investigation of this novel positional candidate gene in BP control in hypertensive rat models and humans.
Title: Genetic dissection of a blood pressure quantitative trait locus on rat chromosome 1 and gene expression analysis identifies SPON1 as a novel candidate hypertension gene.

F-spondin may be an endogenous regulator of APP cleavage.
Title: Binding of F-spondin to amyloid-beta precursor protein: a candidate amyloid-beta precursor protein ligand that modulates amyloid-beta precursor protein cleavage.

The following Spon1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spon1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05952	NM_172067.1 Latest version!	Rattus norvegicus spondin 1 (Spon1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa56825	XM_017589664.1 Latest version!	Rattus norvegicus spondin 1 (Spon1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spon1 ( NM_172067.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742064.1 Rattus norvegicus spondin 1 (Spon1), mRNA.

Price & Availability↑

CloneID	ORa05952
Clone ID Related Accession (Same CDS sequence)	NM_172067.1
Accession Version	NM_172067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	spondin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M88469.1. On Dec 17, 2003 this sequence version replaced XM_346550.1. ##Evidence-Data-START## Transcript exon combination :: M88469.1, BC107655.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCTAT CTCCCGCGCC CCTGAGGCTT AGCCGGGGTC CGGCGCTGCT GGCCCTGGCG 
CTGCCCCTGG CCGCAGCGCT CGCTTTCTCG GATGAGACCC TGGACAAAGT GGCCAAGTCG 
GAGGGCTACT GCAGCCGCAT CTTGCGCGCC CAGGGCACAC GGCGTGAGGG ATACACAGAG 
TTCAGCCTCC GCGTGGAAGG CGACCCTGAC TTCTATAAGC CAGGAAGCAG CTACCGAGTG 
ACACTCTCGG CTGCCCCTCC CTCCTACTTC AGAGGCTTCA CGTTAATTGC TCTCAAAGAG 
AACCGCGAAG GCGATAAGGA AGAAGACCAC GCGGGCACCT TCCAGATCAT AGATGAAGAA 
GAAACCCAGT TTATGAGTAA CTGTCCTGTG GCAGTCACTG AAAGCACCCC TCGGAGGAGG 
ACACGGATCC AGGTGTTTTG GATAGCGCCA CCCACAGGGA CAGGCTGTGT GATTCTGAAG 
GCCAGCATTG TACAGAAACG CATTATCTAT TTTCAAGACG AGGGCTCCCT GACCAAGAAG 
CTGTGTGAAC AGGATCCCAC ACTTGATGGA GTGACGGACA GACCGATCTT AGACTGCTGC 
GCCTGCGGAA CTGCCAAGTA CAGACTCACG TTTTATGGGA ACTGGTCGGA GAAGACTCAT 
CCAAAGGATT ACCCTCGTCG GGCTAATCAC TGGTCTGCCA TCATTGGCGG ATCCCACTCC 
AAGAACTACG TGCTGTGGGA GTACGGAGGG TATGCCAGTG AAGGGGTCAA GCAAGTTGCT 
GAACTTGGCT CACCAGTAAA AATGGAGGAA GAAATTCGAC AACAGAGTGA TGAAGTCCTC 
ACTGTCATCA AAGCCAAAGC CCAGTGGCCA TCCTGGCAGC CTGTCAATGT GAGAGCAGCA 
CCCTCAGCCG AATTCTCAGT GGACAGGACA CGCCACTTGA TGTCCTTCCT AACCATGATG 
GGCCCCAGTC CTGACTGGAA CGTGGGCCTA TCTGCAGAGG ATCTGTGCAC CAAGGAGTGT 
GGCTGGGTCC AGAAAGTGGT GCAGGACCTA ATTCCCTGGG ATGCTGGCAC GGACAGCGGG 
GTGACCTACG AGTCACCAAA CAAGCCCACA ATTCCTCAGG AAAAAATCCG ACCCCTGACT 
AGTCTGGACC ATCCTCAGAG TCCTTTCTAT GACCCGGAAG GTGGGTCCAT CACACAAGTG 
GCCAGAGTCG TCATCGAGAG AATTGCCCGG AAGGGAGAAC AATGCAACAT TGTACCTGAC 
AATGTGGATG ATATTGTAGC CGACCTGGCT CCAGAAGAGA AAGATGAAGA TGACACCCCT 
GAAACCTGCA TCTACTCCAA CTGGTCCCCA TGGTCGGCCT GCAGCTCTTC CACTTGTGAA 
AAGGGTAAGA GGATGCGGCA ACGCATGCTG AAGGCACAGC TGGACCTCAG TGTCCCCTGT 
CCTGACACCC AGGACTTCCA GCCCTGCATG GGCCCCGGCT GCAGCGATGA AGATGGCTCC 
ACCTGTACCA TGTCGGAGTG GATCACCTGG TCACCCTGCA GTGTCTCGTG TGGCATGGGT 
ATGAGGTCCC GGGAGAGGTA CGTGAAGCAG TTCCCGGAAG ACGGCTCGGT GTGCATGCTG 
CCCACGGAAG AGACAGAGAA GTGCACGGTC AACGAGGAGT GCTCTCCTAG CAGCTGCCTG 
GTGACTGAGT GGGGTGAGTG GGATGACTGC AGCGCCACCT GTGGAATGGG CATGAAGAAG 
CGGCACCGCA TGGTCAAGAT GAGCCCCGCG GACGGCTCCA TGTGCAAGGC GGAGACTTCG 
CAGGCGGAGA AATGCATGAT GCCTGAGTGC CATACCATCC CGTGCTTGCT GTCTCCTTGG 
TCCGAGTGGA GCGACTGTAG CGTGACCTGT GGGAAGGGCA TGCGGACGCG CCAGCGGATG 
CTCAAGTCTC TGGCAGAGCT GGGGGACTGT AATGAGGATC TGGAGCAGGC GGAGAAGTGT 
ATGCTGCCAG AGTGCCCCAT TGACTGCGAA CTCAGTGAGT GGTCCCAGTG GTCTGAATGT 
AACAAGTCCT GTGGGAAAGG TCACATGATT CGAACCCGGA CAATCCAAAT GGAACCTCAG 
TTTGGAGGTG CACCCTGCCC AGAGACTGTG CAACGCAAGA AGTGCCGTGC CCGGAAATGC 
CTTCGCAGCC CATCGATCCA GAAGCTGCGC TGGAGGGAGG CCCGAGAGAG CAGGAGGAGT 
GAGCAGCTGA GGGAAGAGTC AGATGGAGAG CAGTTCCCAG GCTGTCGGAT GCGCCCGTGG 
ACAGCCTGGT CAGAGTGCAC CAAACTGTGC GGAGGTGGGA TCCAAGAACG CTACATGACT 
GTGAAGAAGA GGTTCAAAAG CTCCCAGTTT ACCAGCTGCA AAGACAAGAA GGAGATCAGA 
GCGTGCAACG TGCACCCTTG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742064.1
CDS	226..2649
Translation	MRLSPAPLRLSRGPALLALALPLAAALAFSDETLDKVAKSEGYCSRILRAQGTRREGYTEFSLRVEGDPDFYKPGSSYRVTLSAAPPSYFRGFTLIALKENREGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDPTLDGVTDRPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPSWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSVSCGMGMRSRERYVKQFPEDGSVCMLPTEETEKCTVNEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQAEKCMLPECPIDCELSEWSQWSECNKSCGKGHMIRTRTIQMEPQFGGAPCPETVQRKKCRARKCLRSPSIQKLRWREARESRRSEQLREESDGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTVKKRFKSSQFTSCKDKKEIRACNVHPC

Target ORF information:

RefSeq Version	NM_172067.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spondin 1 (Spon1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172067.1

ORF Insert Sequence:

ATGAGGCTAT CTCCCGCGCC CCTGAGGCTT AGCCGGGGTC CGGCGCTGCT GGCCCTGGCG 
CTGCCCCTGG CCGCAGCGCT CGCTTTCTCG GATGAGACCC TGGACAAAGT GGCCAAGTCG 
GAGGGCTACT GCAGCCGCAT CTTGCGCGCC CAGGGCACAC GGCGTGAGGG ATACACAGAG 
TTCAGCCTCC GCGTGGAAGG CGACCCTGAC TTCTATAAGC CAGGAAGCAG CTACCGAGTG 
ACACTCTCGG CTGCCCCTCC CTCCTACTTC AGAGGCTTCA CGTTAATTGC TCTCAAAGAG 
AACCGCGAAG GCGATAAGGA AGAAGACCAC GCGGGCACCT TCCAGATCAT AGATGAAGAA 
GAAACCCAGT TTATGAGTAA CTGTCCTGTG GCAGTCACTG AAAGCACCCC TCGGAGGAGG 
ACACGGATCC AGGTGTTTTG GATAGCGCCA CCCACAGGGA CAGGCTGTGT GATTCTGAAG 
GCCAGCATTG TACAGAAACG CATTATCTAT TTTCAAGACG AGGGCTCCCT GACCAAGAAG 
CTGTGTGAAC AGGATCCCAC ACTTGATGGA GTGACGGACA GACCGATCTT AGACTGCTGC 
GCCTGCGGAA CTGCCAAGTA CAGACTCACG TTTTATGGGA ACTGGTCGGA GAAGACTCAT 
CCAAAGGATT ACCCTCGTCG GGCTAATCAC TGGTCTGCCA TCATTGGCGG ATCCCACTCC 
AAGAACTACG TGCTGTGGGA GTACGGAGGG TATGCCAGTG AAGGGGTCAA GCAAGTTGCT 
GAACTTGGCT CACCAGTAAA AATGGAGGAA GAAATTCGAC AACAGAGTGA TGAAGTCCTC 
ACTGTCATCA AAGCCAAAGC CCAGTGGCCA TCCTGGCAGC CTGTCAATGT GAGAGCAGCA 
CCCTCAGCCG AATTCTCAGT GGACAGGACA CGCCACTTGA TGTCCTTCCT AACCATGATG 
GGCCCCAGTC CTGACTGGAA CGTGGGCCTA TCTGCAGAGG ATCTGTGCAC CAAGGAGTGT 
GGCTGGGTCC AGAAAGTGGT GCAGGACCTA ATTCCCTGGG ATGCTGGCAC GGACAGCGGG 
GTGACCTACG AGTCACCAAA CAAGCCCACA ATTCCTCAGG AAAAAATCCG ACCCCTGACT 
AGTCTGGACC ATCCTCAGAG TCCTTTCTAT GACCCGGAAG GTGGGTCCAT CACACAAGTG 
GCCAGAGTCG TCATCGAGAG AATTGCCCGG AAGGGAGAAC AATGCAACAT TGTACCTGAC 
AATGTGGATG ATATTGTAGC CGACCTGGCT CCAGAAGAGA AAGATGAAGA TGACACCCCT 
GAAACCTGCA TCTACTCCAA CTGGTCCCCA TGGTCGGCCT GCAGCTCTTC CACTTGTGAA 
AAGGGTAAGA GGATGCGGCA ACGCATGCTG AAGGCACAGC TGGACCTCAG TGTCCCCTGT 
CCTGACACCC AGGACTTCCA GCCCTGCATG GGCCCCGGCT GCAGCGATGA AGATGGCTCC 
ACCTGTACCA TGTCGGAGTG GATCACCTGG TCACCCTGCA GTGTCTCGTG TGGCATGGGT 
ATGAGGTCCC GGGAGAGGTA CGTGAAGCAG TTCCCGGAAG ACGGCTCGGT GTGCATGCTG 
CCCACGGAAG AGACAGAGAA GTGCACGGTC AACGAGGAGT GCTCTCCTAG CAGCTGCCTG 
GTGACTGAGT GGGGTGAGTG GGATGACTGC AGCGCCACCT GTGGAATGGG CATGAAGAAG 
CGGCACCGCA TGGTCAAGAT GAGCCCCGCG GACGGCTCCA TGTGCAAGGC GGAGACTTCG 
CAGGCGGAGA AATGCATGAT GCCTGAGTGC CATACCATCC CGTGCTTGCT GTCTCCTTGG 
TCCGAGTGGA GCGACTGTAG CGTGACCTGT GGGAAGGGCA TGCGGACGCG CCAGCGGATG 
CTCAAGTCTC TGGCAGAGCT GGGGGACTGT AATGAGGATC TGGAGCAGGC GGAGAAGTGT 
ATGCTGCCAG AGTGCCCCAT TGACTGCGAA CTCAGTGAGT GGTCCCAGTG GTCTGAATGT 
AACAAGTCCT GTGGGAAAGG TCACATGATT CGAACCCGGA CAATCCAAAT GGAACCTCAG 
TTTGGAGGTG CACCCTGCCC AGAGACTGTG CAACGCAAGA AGTGCCGTGC CCGGAAATGC 
CTTCGCAGCC CATCGATCCA GAAGCTGCGC TGGAGGGAGG CCCGAGAGAG CAGGAGGAGT 
GAGCAGCTGA GGGAAGAGTC AGATGGAGAG CAGTTCCCAG GCTGTCGGAT GCGCCCGTGG 
ACAGCCTGGT CAGAGTGCAC CAAACTGTGC GGAGGTGGGA TCCAAGAACG CTACATGACT 
GTGAAGAAGA GGTTCAAAAG CTCCCAGTTT ACCAGCTGCA AAGACAAGAA GGAGATCAGA 
GCGTGCAACG TGCACCCTTG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spon1 ( XM_017589664.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445153.1 Rattus norvegicus spondin 1 (Spon1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56825
Clone ID Related Accession (Same CDS sequence)	XM_017589664.1
Accession Version	XM_017589664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spondin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGAT GGCCACCACA AAATCTGACA CTCTCGGCTG CCCCTCCCTC CTACTTCAGA 
GGCTTCACGT TAATTGCTCT CAAAGAGAAC CGCGAAGGCG ATAAGGAAGA AGACCACGCG 
GGCACCTTCC AGATCATAGA TGAAGAAGAA ACCCAGTTTA TGAGTAACTG TCCTGTGGCA 
GTCACTGAAA GCACCCCTCG GAGGAGGACA CGGATCCAGG TGTTTTGGAT AGCGCCACCC 
ACAGGGACAG GCTGTGTGAT TCTGAAGGCC AGCATTGTAC AGAAACGCAT TATCTATTTT 
CAAGACGAGG GCTCCCTGAC CAAGAAGCTG TGTGAACAGG ATCCCACACT TGATGGAGTG 
ACGGACAGAC CGATCTTAGA CTGCTGCGCC TGCGGAACTG CCAAGTACAG ACTCACGTTT 
TATGGGAACT GGTCGGAGAA GACTCATCCA AAGGATTACC CTCGTCGGGC TAATCACTGG 
TCTGCCATCA TTGGCGGATC CCACTCCAAG AACTACGTGC TGTGGGAGTA CGGAGGGTAT 
GCCAGTGAAG GGGTCAAGCA AGTTGCTGAA CTTGGCTCAC CAGTAAAAAT GGAGGAAGAA 
ATTCGACAAC AGAGTGATGA AGTCCTCACT GTCATCAAAG CCAAAGCCCA GTGGCCAGCC 
TGGCAGCCTG TCAATGTGAG AGCAGCACCC TCAGCCGAAT TCTCAGTGGA CAGGACACGC 
CACTTGATGT CCTTCCTAAC CATGATGGGC CCCAGTCCTG ACTGGAACGT GGGCCTATCT 
GCAGAGGATC TGTGCACCAA GGAGTGTGGC TGGGTCCAGA AAGTGGTGCA GGACCTAATT 
CCCTGGGATG CTGGCACGGA CAGCGGGGTG ACCTACGAGT CACCAAACAA GCCCACAATT 
CCTCAGGAAA AAATCCGACC CCTGACTAGT CTGGACCATC CTCAGAGTCC TTTCTATGAC 
CCGGAAGGTG GGTCCATCAC ACAAGTGGCC AGAGTCGTCA TCGAGAGAAT TGCCCGGAAG 
GGAGAACAAT GCAACATTGT ACCTGACAAT GTGGATGATA TTGTAGCCGA CCTGGCTCCA 
GAAGAGAAAG ATGAAGATGA CACCCCTGAA ACCTGCATCT ACTCCAACTG GTCCCCATGG 
TCGGCCTGCA GCTCTTCCAC TTGTGAAAAG GGTAAGAGGA TGCGGCAACG CATGCTGAAG 
GCACAGCTGG ACCTCAGTGT CCCCTGTCCT GACACCCAGG ACTTCCAGCC CTGCATGGGC 
CCCGGCTGCA GCGATGAAGA TGGCTCCACC TGTACCATGT CGGAGTGGAT CACCTGGTCA 
CCCTGCAGTG TCTCGTGTGG CATGGGTATG AGGTCCCGGG AGAGGTACGT GAAGCAGTTC 
CCGGAAGACG GCTCGGTGTG CATGCTGCCC ACGGAAGAGA CAGAGAAGTG CACGGTCAAC 
GAGGAGTGCT CTCCTAGCAG CTGCCTGGTG ACTGAGTGGG GTGAGTGGGA TGACTGCAGC 
GCCACCTGTG GAATGGGCAT GAAGAAGCGG CACCGCATGG TCAAGATGAG CCCCGCGGAC 
GGCTCCATGT GCAAGGCGGA GACTTCGCAG GCGGAGAAAT GCATGATGCC TGAGTGCCAT 
ACCATCCCGT GCTTGCTGTC TCCTTGGTCC GAGTGGAGCG ACTGTAGCGT GACCTGTGGG 
AAGGGCATGC GGACGCGCCA GCGGATGCTC AAGTCTCTGG CAGAGCTGGG GGACTGTAAT 
GAGGATCTGG AGCAGGCGGA GAAGTGTATG CTGCCAGAGT GCCCCATTGA CTGCGAACTC 
AGCGAGTGGT CCCAGTGGTC TGAATGTAAC AAGTCCTGTG GGAAAGGTCA CATGATTCGA 
ACCCGGACAA TCCAAATGGA ACCTCAGTTT GGAGGTGCAC CCTGCCCAGA GACTGTGCAA 
CGCAAGAAGT GCCGTGCCCG GAAATGCCTT CGCAGCCCAT CGATCCAGAA GCTGCGCTGG 
AGGGAGGCCC GAGAGAGCAG GAGGAGTGAG CAGCTGAGGG AAGAGTCAGA TGGAGAGCAG 
TTCCCAGGCT GTCGGATGCG CCCGTGGACA GCCTGGTCAG AGTGCACCAA ACTGTGCGGA 
GGTGGGATCC AAGAACGCTA CATGACTGTG AAGAAGAGGT TCAAAAGCTC CCAGTTTACC 
AGCTGCAAAG ACAAGAAGGA GATCAGAGCG TGCAACGTGC ACCCTTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445153.1
CDS	214..2424
Translation	MQRWPPQNLTLSAAPPSYFRGFTLIALKENREGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDPTLDGVTDRPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSVSCGMGMRSRERYVKQFPEDGSVCMLPTEETEKCTVNEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQAEKCMLPECPIDCELSEWSQWSECNKSCGKGHMIRTRTIQMEPQFGGAPCPETVQRKKCRARKCLRSPSIQKLRWREARESRRSEQLREESDGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTVKKRFKSSQFTSCKDKKEIRACNVHPC

Target ORF information:

RefSeq Version	XM_017589664.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spondin 1 (Spon1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589664.1

ORF Insert Sequence:

ATGCAAAGAT GGCCACCACA AAATCTGACA CTCTCGGCTG CCCCTCCCTC CTACTTCAGA 
GGCTTCACGT TAATTGCTCT CAAAGAGAAC CGCGAAGGCG ATAAGGAAGA AGACCACGCG 
GGCACCTTCC AGATCATAGA TGAAGAAGAA ACCCAGTTTA TGAGTAACTG TCCTGTGGCA 
GTCACTGAAA GCACCCCTCG GAGGAGGACA CGGATCCAGG TGTTTTGGAT AGCGCCACCC 
ACAGGGACAG GCTGTGTGAT TCTGAAGGCC AGCATTGTAC AGAAACGCAT TATCTATTTT 
CAAGACGAGG GCTCCCTGAC CAAGAAGCTG TGTGAACAGG ATCCCACACT TGATGGAGTG 
ACGGACAGAC CGATCTTAGA CTGCTGCGCC TGCGGAACTG CCAAGTACAG ACTCACGTTT 
TATGGGAACT GGTCGGAGAA GACTCATCCA AAGGATTACC CTCGTCGGGC TAATCACTGG 
TCTGCCATCA TTGGCGGATC CCACTCCAAG AACTACGTGC TGTGGGAGTA CGGAGGGTAT 
GCCAGTGAAG GGGTCAAGCA AGTTGCTGAA CTTGGCTCAC CAGTAAAAAT GGAGGAAGAA 
ATTCGACAAC AGAGTGATGA AGTCCTCACT GTCATCAAAG CCAAAGCCCA GTGGCCAGCC 
TGGCAGCCTG TCAATGTGAG AGCAGCACCC TCAGCCGAAT TCTCAGTGGA CAGGACACGC 
CACTTGATGT CCTTCCTAAC CATGATGGGC CCCAGTCCTG ACTGGAACGT GGGCCTATCT 
GCAGAGGATC TGTGCACCAA GGAGTGTGGC TGGGTCCAGA AAGTGGTGCA GGACCTAATT 
CCCTGGGATG CTGGCACGGA CAGCGGGGTG ACCTACGAGT CACCAAACAA GCCCACAATT 
CCTCAGGAAA AAATCCGACC CCTGACTAGT CTGGACCATC CTCAGAGTCC TTTCTATGAC 
CCGGAAGGTG GGTCCATCAC ACAAGTGGCC AGAGTCGTCA TCGAGAGAAT TGCCCGGAAG 
GGAGAACAAT GCAACATTGT ACCTGACAAT GTGGATGATA TTGTAGCCGA CCTGGCTCCA 
GAAGAGAAAG ATGAAGATGA CACCCCTGAA ACCTGCATCT ACTCCAACTG GTCCCCATGG 
TCGGCCTGCA GCTCTTCCAC TTGTGAAAAG GGTAAGAGGA TGCGGCAACG CATGCTGAAG 
GCACAGCTGG ACCTCAGTGT CCCCTGTCCT GACACCCAGG ACTTCCAGCC CTGCATGGGC 
CCCGGCTGCA GCGATGAAGA TGGCTCCACC TGTACCATGT CGGAGTGGAT CACCTGGTCA 
CCCTGCAGTG TCTCGTGTGG CATGGGTATG AGGTCCCGGG AGAGGTACGT GAAGCAGTTC 
CCGGAAGACG GCTCGGTGTG CATGCTGCCC ACGGAAGAGA CAGAGAAGTG CACGGTCAAC 
GAGGAGTGCT CTCCTAGCAG CTGCCTGGTG ACTGAGTGGG GTGAGTGGGA TGACTGCAGC 
GCCACCTGTG GAATGGGCAT GAAGAAGCGG CACCGCATGG TCAAGATGAG CCCCGCGGAC 
GGCTCCATGT GCAAGGCGGA GACTTCGCAG GCGGAGAAAT GCATGATGCC TGAGTGCCAT 
ACCATCCCGT GCTTGCTGTC TCCTTGGTCC GAGTGGAGCG ACTGTAGCGT GACCTGTGGG 
AAGGGCATGC GGACGCGCCA GCGGATGCTC AAGTCTCTGG CAGAGCTGGG GGACTGTAAT 
GAGGATCTGG AGCAGGCGGA GAAGTGTATG CTGCCAGAGT GCCCCATTGA CTGCGAACTC 
AGCGAGTGGT CCCAGTGGTC TGAATGTAAC AAGTCCTGTG GGAAAGGTCA CATGATTCGA 
ACCCGGACAA TCCAAATGGA ACCTCAGTTT GGAGGTGCAC CCTGCCCAGA GACTGTGCAA 
CGCAAGAAGT GCCGTGCCCG GAAATGCCTT CGCAGCCCAT CGATCCAGAA GCTGCGCTGG 
AGGGAGGCCC GAGAGAGCAG GAGGAGTGAG CAGCTGAGGG AAGAGTCAGA TGGAGAGCAG 
TTCCCAGGCT GTCGGATGCG CCCGTGGACA GCCTGGTCAG AGTGCACCAA ACTGTGCGGA 
GGTGGGATCC AAGAACGCTA CATGACTGTG AAGAAGAGGT TCAAAAGCTC CCAGTTTACC 
AGCTGCAAAG ACAAGAAGGA GATCAGAGCG TGCAACGTGC ACCCTTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomics characterization of extracellular space components in the human aorta.
Molecular & cellular proteomics : MCP9(9)2048-62(2010 Sep)
Didangelos A,Yin X,Mandal K,Baumert M,Jahangiri M,Mayr M

Genetic dissection of a blood pressure quantitative trait locus on rat chromosome 1 and gene expression analysis identifies SPON1 as a novel candidate hypertension gene.
Circulation research100(7)992-9(2007 Apr)
Clemitson JR,Dixon RJ,Haines S,Bingham AJ,Patel BR,Hall L,Lo M,Sassard J,Charchar FJ,Samani NJ

F-spondin interaction with the apolipoprotein E receptor ApoEr2 affects processing of amyloid precursor protein.
Molecular and cellular biology25(21)9259-68(2005 Nov)
Hoe HS,Wessner D,Beffert U,Becker AG,Matsuoka Y,Rebeck GW

Binding of F-spondin to amyloid-beta precursor protein: a candidate amyloid-beta precursor protein ligand that modulates amyloid-beta precursor protein cleavage.
Proceedings of the National Academy of Sciences of the United States of America101(8)2548-53(2004 Feb)
Ho A,S?dhof TC

F-spondin and mindin: two structurally and functionally related genes expressed in the hippocampus that promote outgrowth of embryonic hippocampal neurons.
Development (Cambridge, England)126(16)3637-48(1999 Aug)
Feinstein Y,Borrell V,Garcia C,Burstyn-Cohen T,Tzarfaty V,Frumkin A,Nose A,Okamoto H,Higashijima S,Soriano E,Klar A

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Spon1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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