Defb4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Defb4
Entrez Gene ID	64389
Full Name	defensin beta 4
Synonyms	Defb2,Defb3
General protein information	Preferred Names defensin beta 4 Names beta-defensin 4 BD-2 BD-4 RBD-2 RBD-4 beta defensin-2 beta-defensin 3 defensin beta 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.5
Summary	cationic peptides with broad-spectrum antimicrobial activity; may mediate mucosal defenses of several organs [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022544.2	NP_071989.1	beta-defensin 4 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Protective role of murine ?-defensins 3 and 4 and cathelin-related antimicrobial peptide in Fusarium solani keratitis.
Kolar SS, Baidouri H, Hanlon S, McDermott AM
Infection and immunity81(8)2669-77(2013 Aug)

The chromosomal protein HMGN2 mediates lipopolysaccharide-induced expression of ?-defensins in A549 cells.
Deng LX, Wu GX, Cao Y, Fan B, Gao X, Luo L, Huang N
The FEBS journal278(12)2152-66(2011 Jun)

Role of defensins in corneal epithelial barrier function against Pseudomonas aeruginosa traversal.
Augustin DK, Heimer SR, Tam C, Li WY, Le Due JM, Evans DJ, Fleiszig SM
Infection and immunity79(2)595-605(2011 Feb)

Cigarette smoke enhances {beta}-defensin 2 expression in rat airways via nuclear factor-{kappa}B activation.
Chen L, Sun BB, Wang T, Wang X, Li JQ, Wang HX, Zhang SF, Liu DS, Liu L, Xu D, Ou XM, Chen YJ, Yang T, Zhou H, Wen FQ
The European respiratory journal36(3)638-45(2010 Sep)

Specific binding and chemotactic activity of mBD4 and its functional orthologue hBD2 to CCR6-expressing cells.
R?hrl J, Yang D, Oppenheim JJ, Hehlgans T
The Journal of biological chemistry285(10)7028-34(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HMGN2 acts as a positive modulator of nuclear factor kappaB signalling to promote lipopolysaccharide-induced beta-defensin-2 expression.
Title: The chromosomal protein HMGN2 mediates lipopolysaccharide-induced expression of ?-defensins in A549 cells.

Cigarette smoke enhances {beta}-defensin 2 expression in rat airways via nuclear factor-{kappa}B activation
Title: Cigarette smoke enhances {beta}-defensin 2 expression in rat airways via nuclear factor-{kappa}B activation.

Expression of beta-defensin-2 mRNA was primarily restricted to the penis.
Title: Messenger RNA (mRNA) expression for the antimicrobial peptides beta-defensin-1 and beta-defensin-2 in the male rat reproductive tract: beta-defensin-1 mRNA in initial segment and caput epididymidis is regulated by androgens and not bacterial lipopolysaccharides.

The following Defb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Defb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05894	NM_022544.2 Latest version!	Rattus norvegicus defensin beta 4 (Defb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Defb4 ( NM_022544.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071989.1 Rattus norvegicus defensin beta 4 (Defb4), mRNA.

Price & Availability↑

CloneID	ORa05894
Clone ID Related Accession (Same CDS sequence)	NM_022544.2
Accession Version	NM_022544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	beta-defensin 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY621374.1. On Nov 27, 2005 this sequence version replaced NM_022544.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY621374.1, AF068861.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689605 [ECO:0000348] ##Evidence-Data-END##

ATGAGGATCC ATTACCTCCT CTTCTCATTT CTCCTGGTGC TGCTGTCGCC TCTTTCAGCC 
TTTACTCAAA GTATCAACAA TCCAATCACA TGCCTGACCA AAGGAGGCGT ATGCTGGGGT 
CCATGCACTG GCGGTTTTCG ACAGATTGGC ACTTGTGGAC TGCCTAGAGT GAGATGCTGC 
AAGAAAAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071989.1
CDS	139..330
Translation	MRIHYLLFSFLLVLLSPLSAFTQSINNPITCLTKGGVCWGPCTGGFRQIGTCGLPRVRCCKKK

Target ORF information:

RefSeq Version	NM_022544.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus defensin beta 4 (Defb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022544.2

ORF Insert Sequence:

ATGAGGATCC ATTACCTCCT CTTCTCATTT CTCCTGGTGC TGCTGTCGCC TCTTTCAGCC 
TTTACTCAAA GTATCAACAA TCCAATCACA TGCCTGACCA AAGGAGGCGT ATGCTGGGGT 
CCATGCACTG GCGGTTTTCG ACAGATTGGC ACTTGTGGAC TGCCTAGAGT GAGATGCTGC 
AAGAAAAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protective role of murine ?-defensins 3 and 4 and cathelin-related antimicrobial peptide in Fusarium solani keratitis.
Infection and immunity81(8)2669-77(2013 Aug)
Kolar SS,Baidouri H,Hanlon S,McDermott AM

The chromosomal protein HMGN2 mediates lipopolysaccharide-induced expression of ?-defensins in A549 cells.
The FEBS journal278(12)2152-66(2011 Jun)
Deng LX,Wu GX,Cao Y,Fan B,Gao X,Luo L,Huang N

Role of defensins in corneal epithelial barrier function against Pseudomonas aeruginosa traversal.
Infection and immunity79(2)595-605(2011 Feb)
Augustin DK,Heimer SR,Tam C,Li WY,Le Due JM,Evans DJ,Fleiszig SM

Cigarette smoke enhances {beta}-defensin 2 expression in rat airways via nuclear factor-{kappa}B activation.
The European respiratory journal36(3)638-45(2010 Sep)
Chen L,Sun BB,Wang T,Wang X,Li JQ,Wang HX,Zhang SF,Liu DS,Liu L,Xu D,Ou XM,Chen YJ,Yang T,Zhou H,Wen FQ

Specific binding and chemotactic activity of mBD4 and its functional orthologue hBD2 to CCR6-expressing cells.
The Journal of biological chemistry285(10)7028-34(2010 Mar)
R?hrl J,Yang D,Oppenheim JJ,Hehlgans T