Calr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Calr
Entrez Gene ID	64202
Full Name	calreticulin
General protein information	Preferred Names calreticulin Names calreticulin CABP3 CALBP CRP55 ERp60 HACBP calcium-binding protein 3 calregulin endoplasmic reticulum resident protein 60
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11
Summary	Ca(2+)-binding molecular chaperone that promotes folding of proteins carrying N-linked glycans [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022399.2	NP_071794.1	calreticulin precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CALR	NP_776425.1
Danio rerio (zebrafish)	calrl2	NP_956007.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004212	XP_313116.1
Arabidopsis thaliana (thale cress)	CRT1b	NP_172392.1
Mus musculus (house mouse)	Calr	NP_031617.1
Rattus norvegicus (Norway rat)	Calr	NP_071794.1
Gallus gallus (chicken)	CALR3	XP_418262.3
Arabidopsis thaliana (thale cress)	CRT1a	NP_176030.1
Homo sapiens (human)	CALR	NP_004334.1
Oryza sativa (rice)	Os03g0832200	NP_001051799.1
Pan troglodytes (chimpanzee)	CALR	XP_003316194.1
Canis lupus familiaris (dog)	CALR	XP_867310.2
Caenorhabditis elegans (roundworm)	crt-1	NP_504575.1
Oryza sativa (rice)	Os07g0246200	NP_001059283.1
Xenopus tropicalis (tropical clawed frog)	calr	NP_001001253.1
	CALR	XP_001110217.1
Drosophila melanogaster (fruit fly)	Crc	NP_001262430.1

Gene Ontology
Bibliography

Related articles in PubMed

Stratified analysis of lectin-like chaperones in the folding disease-related metabolic syndrome rat model.
Hirano M, Imagawa A, Totani K
Biochemical and biophysical research communications478(1)247-253(2016 09)

Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.
Banushi B, Forneris F, Straatman-Iwanowska A, Strange A, Lyne AM, Rogerson C, Burden JJ, Heywood WE, Hanley J, Doykov I, Straatman KR, Smith H, Bem D, Kriston-Vizi J, Ariceta G, Risteli M, Wang C, Ardill RE, Zaniew M, Latka-Grot J, Waddington SN, Howe SJ, Ferraro F, Gjinovci A, Lawrence S, Marsh M, Girolami M, Bozec L, Mills K, Gissen P
Nature communications712111(2016 Jul)

Homozygous calreticulin mutations in patients with myelofibrosis lead to acquired myeloperoxidase deficiency.
Theocharides AP, Lundberg P, Lakkaraju AK, Lysenko V, Myburgh R, Aguzzi A, Skoda RC, Manz MG
Blood127(25)3253-9(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Therefore, these results indicate that obesity down-regulates CNX and CRT expressions and their activities and diabetes up-regulates the expressions and activities of CNX and CRT. Our findings clearly suggest that metabolic syndrome affects the lectin-like chaperones in the CNX/CRT cycle at both the activity and expression levels.
Title: Stratified analysis of lectin-like chaperones in the folding disease-related metabolic syndrome rat model.

Correlation analysis revealed that integrin-alpha expression or FAK phosphorylation was positively associated with cytosolic CRT expression.
Title: Cytosolic calreticulin inhibits microwave radiation-induced microvascular endothelial cell injury through the integrin-focal adhesion kinase pathway.

Exogenous CRT attenuates MR-induced ER stress-related apoptosis by suppressing CHOP-mediated apoptotic signaling pathways in MMECs.
Title: Calreticulin protects rat microvascular endothelial cells against microwave radiation-induced injury by attenuating endoplasmic reticulum stress.

These data demonstrate that CRT is localized at cardiomyocyte mitochondria and its mitochondrial content is increased in DCM hearts.
Title: Novel distribution of calreticulin to cardiomyocyte mitochondria and its increase in a rat model of dilated cardiomyopathy.

Data highlights the potential importance of CRT and other endoplasmic reticulum stress proteins in inflammatory demyelination
Title: Calreticulin and other components of endoplasmic reticulum stress in rat and human inflammatory demyelination.

The following Calr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Calr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12902	NM_022399.2 Latest version!	Rattus norvegicus calreticulin (Calr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Calr ( NM_022399.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071794.1 Rattus norvegicus calreticulin (Calr), mRNA.

Price & Availability↑

CloneID	ORa12902
Clone ID Related Accession (Same CDS sequence)	NM_022399.2
Accession Version	NM_022399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	calreticulin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU671467.1, BC062395.1 and AW672583.1. On Jan 24, 2008 this sequence version replaced NM_022399.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D78308.1, FQ210587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCTCCTTT CGGTGCCGCT CCTGCTTGGC CTCCTCGGCC TGGCTGCCGC AGACCCTGCC 
ATCTATTTCA AAGAGCAGTT CTTGGACGGA GATGCCTGGA CCAACCGCTG GGTCGAATCC 
AAACATAAGT CTGATTTTGG CAAATTCGTC CTCAGTTCTG GCAAATTCTA CGGGGACCAG 
GAGAAGGATA AAGGGTTGCA GACAAGCCAA GATGCCCGAT TTTACGCGCT GTCCGCCAGA 
TTCGAACCCT TCAGCAACAA GGGCCAGACA CTGGTGGTAC AGTTCACCGT GAAGCATGAG 
CAGAATATCG ACTGTGGGGG CGGCTACGTG AAGCTGTTTC CGGGTGGCTT GGACCAGAAG 
GACATGCATG GAGACTCAGA ATATAACATC ATGTTTGGTC CGGACATCTG CGGTCCTGGC 
ACCAAGAAGG TTCATGTCAT CTTTAACTAC AAGGGCAAGA ACGTGCTGAT CAACAAGGAT 
ATCCGGTGTA AGGATGATGA ATTCACACAT CTATACACGC TGATTGTGCG GCCAGACAAC 
ACCTACGAGG TGAAAATTGA CAACAGCCAG GTGGAGTCGG GCTCCTTGGA GGATGATTGG 
GACTTTCTGC CGCCCAAGAA GATTAAGGAT CCTGACGCTG CCAAGCCAGA AGACTGGGAT 
GAACGAGCCA AGATTGATGA CCCCACAGAT TCCAAGCCTG AGGACTGGGA CAAGCCAGAG 
CACATCCCTG ACCCTGATGC TAAGAAGCCT GAGGACTGGG ACGAAGAGAT GGATGGAGAG 
TGGGAACCAC CAGTGATTCA AAATCCTGAA TACAAGGGCG AATGGAAGCC ACGTCAAATT 
GACAACCCAG ATTACAAGGG TACCTGGATA CACCCAGAGA TTGACAATCC TGAATACTCC 
CCCGATGCGA ATATCTATGC CTATGATAGT TTTGCTGTAC TGGGCTTAGA CCTCTGGCAG 
GTCAAGTCTG GCACAATTTT TGACAACTTC CTCATCACCA ATGATGAGGC CTATGCAGAG 
GAGTTTGGCA ATGAGACCTG GGGTGTCACC AAGGCTGCAG AGAAGCAGAT GAAGGACAAG 
CAGGATGAGG AGCAGAGGCT TAAGGAAGAA GAAGAAGACA AGAAGCGTAA AGAGGAAGAG 
GAGGCCGAGG ATAAAGAGGA TGAGGATGAC AGAGATGAAG ATGAAGATGA AGAGGATGAG 
AAGGAAGAAG ATGAGGAGGA TGCCACTGGC CAAGCCAAGG ATGAGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071794.1
CDS	64..1314
Translation	MLLSVPLLLGLLGLAAADPAIYFKEQFLDGDAWTNRWVESKHKSDFGKFVLSSGKFYGDQEKDKGLQTSQDARFYALSARFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPGGLDQKDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKDPDAAKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDANIYAYDSFAVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDEDDRDEDEDEEDEKEEDEEDATGQAKDEL

Target ORF information:

RefSeq Version	NM_022399.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calreticulin (Calr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022399.2

ORF Insert Sequence:

ATGCTCCTTT CGGTGCCGCT CCTGCTTGGC CTCCTCGGCC TGGCTGCCGC AGACCCTGCC 
ATCTATTTCA AAGAGCAGTT CTTGGACGGA GATGCCTGGA CCAACCGCTG GGTCGAATCC 
AAACATAAGT CTGATTTTGG CAAATTCGTC CTCAGTTCTG GCAAATTCTA CGGGGACCAG 
GAGAAGGATA AAGGGTTGCA GACAAGCCAA GATGCCCGAT TTTACGCGCT GTCCGCCAGA 
TTCGAACCCT TCAGCAACAA GGGCCAGACA CTGGTGGTAC AGTTCACCGT GAAGCATGAG 
CAGAATATCG ACTGTGGGGG CGGCTACGTG AAGCTGTTTC CGGGTGGCTT GGACCAGAAG 
GACATGCATG GAGACTCAGA ATATAACATC ATGTTTGGTC CGGACATCTG CGGTCCTGGC 
ACCAAGAAGG TTCATGTCAT CTTTAACTAC AAGGGCAAGA ACGTGCTGAT CAACAAGGAT 
ATCCGGTGTA AGGATGATGA ATTCACACAT CTATACACGC TGATTGTGCG GCCAGACAAC 
ACCTACGAGG TGAAAATTGA CAACAGCCAG GTGGAGTCGG GCTCCTTGGA GGATGATTGG 
GACTTTCTGC CGCCCAAGAA GATTAAGGAT CCTGACGCTG CCAAGCCAGA AGACTGGGAT 
GAACGAGCCA AGATTGATGA CCCCACAGAT TCCAAGCCTG AGGACTGGGA CAAGCCAGAG 
CACATCCCTG ACCCTGATGC TAAGAAGCCT GAGGACTGGG ACGAAGAGAT GGATGGAGAG 
TGGGAACCAC CAGTGATTCA AAATCCTGAA TACAAGGGCG AATGGAAGCC ACGTCAAATT 
GACAACCCAG ATTACAAGGG TACCTGGATA CACCCAGAGA TTGACAATCC TGAATACTCC 
CCCGATGCGA ATATCTATGC CTATGATAGT TTTGCTGTAC TGGGCTTAGA CCTCTGGCAG 
GTCAAGTCTG GCACAATTTT TGACAACTTC CTCATCACCA ATGATGAGGC CTATGCAGAG 
GAGTTTGGCA ATGAGACCTG GGGTGTCACC AAGGCTGCAG AGAAGCAGAT GAAGGACAAG 
CAGGATGAGG AGCAGAGGCT TAAGGAAGAA GAAGAAGACA AGAAGCGTAA AGAGGAAGAG 
GAGGCCGAGG ATAAAGAGGA TGAGGATGAC AGAGATGAAG ATGAAGATGA AGAGGATGAG 
AAGGAAGAAG ATGAGGAGGA TGCCACTGGC CAAGCCAAGG ATGAGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stratified analysis of lectin-like chaperones in the folding disease-related metabolic syndrome rat model.
Biochemical and biophysical research communications478(1)247-253(2016 09)
Hirano M,Imagawa A,Totani K

Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.
Nature communications712111(2016 Jul)
Banushi B,Forneris F,Straatman-Iwanowska A,Strange A,Lyne AM,Rogerson C,Burden JJ,Heywood WE,Hanley J,Doykov I,Straatman KR,Smith H,Bem D,Kriston-Vizi J,Ariceta G,Risteli M,Wang C,Ardill RE,Zaniew M,Latka-Grot J,Waddington SN,Howe SJ,Ferraro F,Gjinovci A,Lawrence S,Marsh M,Girolami M,Bozec L,Mills K,Gissen P

Homozygous calreticulin mutations in patients with myelofibrosis lead to acquired myeloperoxidase deficiency.
Blood127(25)3253-9(2016 06)
Theocharides AP,Lundberg P,Lakkaraju AK,Lysenko V,Myburgh R,Aguzzi A,Skoda RC,Manz MG

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Calr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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