Pag1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pag1
Entrez Gene ID	64019
Full Name	phosphoprotein membrane anchor with glycosphingolipid microdomains 1
Synonyms	Cbp
General protein information	Preferred Names phosphoprotein membrane anchor with glycosphingolipid microdomains 1 Names phosphoprotein associated with glycosphingolipid-enriched microdomains 1 Csk binding protein csk-binding protein phosphoprotein associated with glycosphingolipid microdomains 1 transmembrane phosphoprotein Cbp
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q23
Summary	plays a role in negative regulation of FcepsilonRI signaling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022253.1	NP_071589.1	phosphoprotein associated with glycosphingolipid-enriched microdomains 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PAG1	XP_001168080.1
	PAG1	XP_001091544.1
Bos taurus (cattle)	PAG1	XP_002692759.1
Danio rerio (zebrafish)	pag1	XP_003200660.1
Homo sapiens (human)	PAG1	NP_060910.3
Gallus gallus (chicken)	PAG1	XP_004939990.1
Canis lupus familiaris (dog)	PAG1	XP_853555.1
Mus musculus (house mouse)	Pag1	NP_001181960.1
Rattus norvegicus (Norway rat)	Pag1	NP_071589.1
Xenopus tropicalis (tropical clawed frog)	pag1	XP_004915221.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a new interaction mode between the Src homology 2 domain of C-terminal Src kinase (Csk) and Csk-binding protein/phosphoprotein associated with glycosphingolipid microdomains.
Tanaka H, Akagi K, Oneyama C, Tanaka M, Sasaki Y, Kanou T, Lee YH, Yokogawa D, Dobenecker MW, Nakagawa A, Okada M, Ikegami T
The Journal of biological chemistry288(21)15240-54(2013 May)

CSK negatively regulates nerve growth factor induced neural differentiation and augments AKT kinase activity.
Dey N, Howell BW, De PK, Durden DL
Experimental cell research307(1)1-14(2005 Jul)

Mechanism of Csk-mediated down-regulation of Src family tyrosine kinases in epidermal growth factor signaling.
Matsuoka H, Nada S, Okada M
The Journal of biological chemistry279(7)5975-83(2004 Feb)

Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor.
Davidson D, Bakinowski M, Thomas ML, Horejsi V, Veillette A
Molecular and cellular biology23(6)2017-28(2003 Mar)

Cutting Edge: Transmembrane phosphoprotein Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomains as a negative feedback regulator of mast cell signaling through the FcepsilonRI.
Ohtake H, Ichikawa N, Okada M, Yamashita T
Journal of immunology (Baltimore, Md. : 1950)168(5)2087-90(2002 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

an allosteric connection between Cbp binding and Csk activation that arises from residues in the betaB/betaC loop of the SH2 domain.
Title: Identification of a new interaction mode between the Src homology 2 domain of C-terminal Src kinase (Csk) and Csk-binding protein/phosphoprotein associated with glycosphingolipid microdomains.

Csk negatively regulates growth factor induced neural differentiation and augments Akt kinase activity.
Title: CSK negatively regulates nerve growth factor induced neural differentiation and augments AKT kinase activity.

negative feedback regulator of mast cell signaling through the FcepsilonRI
Title: Cutting Edge: Transmembrane phosphoprotein Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomains as a negative feedback regulator of mast cell signaling through the FcepsilonRI.

The following Pag1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pag1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13769	NM_022253.1 Latest version!	Rattus norvegicus phosphoprotein membrane anchor with glycosphingolipid microdomains 1 (Pag1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pag1 ( NM_022253.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071589.1 Rattus norvegicus phosphoprotein membrane anchor with glycosphingolipid microdomains 1 (Pag1), mRNA.

Price & Availability↑

CloneID	ORa13769
Clone ID Related Accession (Same CDS sequence)	NM_022253.1
Accession Version	NM_022253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	phosphoprotein associated with glycosphingolipid-enriched microdomains 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB038038.1. ##Evidence-Data-START## Transcript exon combination :: AB038038.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGACCTG CAGGAAGCGC CCTGAGCAGC GGGCAGATGC AGATGCAGAT GGTGCTGTGG 
GGAAGTCTGG CTGCGGTGGC CATGTTCTTC CTCATCACCT TCCTCATCCT TCTGTGCTCC 
AGTTGTGACA GAGACAAGAA GCCACGGCAG CATAGTGGAG ACCATGAGAG CCTGATGAAT 
GTGCCTTCCG ACAAGGAGAT GTTCAGCCAT TCAGCGACCA GCCTGACCAC AGATGCACTG 
GCCAGCAGCG AACAGAATGG GGTGCTCACC AACGGCGACA TTCTTTCAGA AGACAGCACA 
ATGACCTGCA TGCAGCATTA TGAGGAAGTC CAGACCTCAG CTTCAGATCT GCTGGACTCC 
CAGGACAGCA CGGGAAAGGC CAAGTGCCAC CAGAGCCGGG AACTGCCCAG AATCCCGCCT 
GAGAACGCAG TGGACGCGAT GCTCACCGCC AGGGCCGCAG ATGGGGACTC CGGGCCCGGC 
GTGGAGGGGC CCTACGAGGT GCTCAAGGAT AGTTCCTCCC AGGAGAACAT GGTGGAGGAC 
TGCTTGTATG AGACAGTGAA GGAAATCAAG GAGGTGGCAG ACAAAAGCCA GGGCGGCAAG 
TCCAAGTCCA CTTCTGCCCT GAAGGAGCTT CAGGGGGCCC ACGCCGAGGG CAAGGCCGAC 
TTTGCTGAAT ACGCCTCTGT GGACAGAAAC AAAAAGTGCC GCCACAGCAC AAATGCAGAG 
AGCATTCTGG GAACGTCCAG TGACCTGGAT GAGGAAACCC CACCGCCTGT CCCAGTTAAA 
CTTCTGGATG AGAATGCCAA CCTTCCAGAG AAGGGAGAGC ACGGGGCAGA AGAGCAAGCT 
CCAGAAGCAC CCAGTGGACA CAGCAAGAGA TTTAGTTCCT TGTCATACAA GTCTCGAGAA 
GAAGACCCAA CTCTTACAGA AGAGGAGATC TCAGCAATGT ATTCTTCAGT GAATAAGCCT 
GGACAATCGG CACACAAACC TGGGCCGGAA TCTACCTGCC AGTGCCCGCA GGGTCCCCCT 
CAGAGGTCAT CATCTTCCTG TAACGACCTC TATGCCACTG TGAAAGACTT CGAGAAAACT 
CCCAACAGCA TCAGCATGCT GCCACCAGCA AGGAGGCCCG GTGAGGAACC AGAGCCTGAC 
TATGAAGCCA TACAGACTCT AAACAGAGAG GACGACAAGG TCCCTCTGGG GACCAATGGC 
CACCTTGTCC CCAAGGAGAA TGACTATGAG AGCATTGGGG ACTTGCAGCA AGGCAGGGAT 
GTCACCAGGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071589.1
CDS	75..1349
Translation	MGPAGSALSSGQMQMQMVLWGSLAAVAMFFLITFLILLCSSCDRDKKPRQHSGDHESLMNVPSDKEMFSHSATSLTTDALASSEQNGVLTNGDILSEDSTMTCMQHYEEVQTSASDLLDSQDSTGKAKCHQSRELPRIPPENAVDAMLTARAADGDSGPGVEGPYEVLKDSSSQENMVEDCLYETVKEIKEVADKSQGGKSKSTSALKELQGAHAEGKADFAEYASVDRNKKCRHSTNAESILGTSSDLDEETPPPVPVKLLDENANLPEKGEHGAEEQAPEAPSGHSKRFSSLSYKSREEDPTLTEEEISAMYSSVNKPGQSAHKPGPESTCQCPQGPPQRSSSSCNDLYATVKDFEKTPNSISMLPPARRPGEEPEPDYEAIQTLNREDDKVPLGTNGHLVPKENDYESIGDLQQGRDVTRL

Target ORF information:

RefSeq Version	NM_022253.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoprotein membrane anchor with glycosphingolipid microdomains 1 (Pag1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022253.1

ORF Insert Sequence:

ATGGGACCTG CAGGAAGCGC CCTGAGCAGC GGGCAGATGC AGATGCAGAT GGTGCTGTGG 
GGAAGTCTGG CTGCGGTGGC CATGTTCTTC CTCATCACCT TCCTCATCCT TCTGTGCTCC 
AGTTGTGACA GAGACAAGAA GCCACGGCAG CATAGTGGAG ACCATGAGAG CCTGATGAAT 
GTGCCTTCCG ACAAGGAGAT GTTCAGCCAT TCAGCGACCA GCCTGACCAC AGATGCACTG 
GCCAGCAGCG AACAGAATGG GGTGCTCACC AACGGCGACA TTCTTTCAGA AGACAGCACA 
ATGACCTGCA TGCAGCATTA TGAGGAAGTC CAGACCTCAG CTTCAGATCT GCTGGACTCC 
CAGGACAGCA CGGGAAAGGC CAAGTGCCAC CAGAGCCGGG AACTGCCCAG AATCCCGCCT 
GAGAACGCAG TGGACGCGAT GCTCACCGCC AGGGCCGCAG ATGGGGACTC CGGGCCCGGC 
GTGGAGGGGC CCTACGAGGT GCTCAAGGAT AGTTCCTCCC AGGAGAACAT GGTGGAGGAC 
TGCTTGTATG AGACAGTGAA GGAAATCAAG GAGGTGGCAG ACAAAAGCCA GGGCGGCAAG 
TCCAAGTCCA CTTCTGCCCT GAAGGAGCTT CAGGGGGCCC ACGCCGAGGG CAAGGCCGAC 
TTTGCTGAAT ACGCCTCTGT GGACAGAAAC AAAAAGTGCC GCCACAGCAC AAATGCAGAG 
AGCATTCTGG GAACGTCCAG TGACCTGGAT GAGGAAACCC CACCGCCTGT CCCAGTTAAA 
CTTCTGGATG AGAATGCCAA CCTTCCAGAG AAGGGAGAGC ACGGGGCAGA AGAGCAAGCT 
CCAGAAGCAC CCAGTGGACA CAGCAAGAGA TTTAGTTCCT TGTCATACAA GTCTCGAGAA 
GAAGACCCAA CTCTTACAGA AGAGGAGATC TCAGCAATGT ATTCTTCAGT GAATAAGCCT 
GGACAATCGG CACACAAACC TGGGCCGGAA TCTACCTGCC AGTGCCCGCA GGGTCCCCCT 
CAGAGGTCAT CATCTTCCTG TAACGACCTC TATGCCACTG TGAAAGACTT CGAGAAAACT 
CCCAACAGCA TCAGCATGCT GCCACCAGCA AGGAGGCCCG GTGAGGAACC AGAGCCTGAC 
TATGAAGCCA TACAGACTCT AAACAGAGAG GACGACAAGG TCCCTCTGGG GACCAATGGC 
CACCTTGTCC CCAAGGAGAA TGACTATGAG AGCATTGGGG ACTTGCAGCA AGGCAGGGAT 
GTCACCAGGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of a new interaction mode between the Src homology 2 domain of C-terminal Src kinase (Csk) and Csk-binding protein/phosphoprotein associated with glycosphingolipid microdomains.
The Journal of biological chemistry288(21)15240-54(2013 May)
Tanaka H,Akagi K,Oneyama C,Tanaka M,Sasaki Y,Kanou T,Lee YH,Yokogawa D,Dobenecker MW,Nakagawa A,Okada M,Ikegami T

CSK negatively regulates nerve growth factor induced neural differentiation and augments AKT kinase activity.
Experimental cell research307(1)1-14(2005 Jul)
Dey N,Howell BW,De PK,Durden DL

Mechanism of Csk-mediated down-regulation of Src family tyrosine kinases in epidermal growth factor signaling.
The Journal of biological chemistry279(7)5975-83(2004 Feb)
Matsuoka H,Nada S,Okada M

Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor.
Molecular and cellular biology23(6)2017-28(2003 Mar)
Davidson D,Bakinowski M,Thomas ML,Horejsi V,Veillette A

Cutting Edge: Transmembrane phosphoprotein Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomains as a negative feedback regulator of mast cell signaling through the FcepsilonRI.
Journal of immunology (Baltimore, Md. : 1950)168(5)2087-90(2002 Mar)
Ohtake H,Ichikawa N,Okada M,Yamashita T

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Pag1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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